CN102754624B - Method for delaying hatching of F1 hybrids of silkworm eggs in hatching - Google Patents
Method for delaying hatching of F1 hybrids of silkworm eggs in hatching Download PDFInfo
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Abstract
The invention discloses a method for delaying the hatching of F1 hybrids of incubated silkworm eggs in hatching. The F1 hybrids of silkworm eggs in hatching are subjected to three-section type low-temperature preservation, and graine embryos of the F1 hybrids of silkworm eggs of mulberry silkworms are grown through the hatching processing to a body pigmentation stage, and then are continuously preserved at the certain environment temperature; 10 silkworm eggs boxes are bundled by a plastic rope, and put in a double-layered black cloth bag wrung after being soaked in water, and the silkworm eggs in the 10 silkworm eggs boxes are uniformly shaken and horizontally placed; and the black cloth bag wrapping the silkworm eggs boxes is put in a certain temperature environment for preservation, and then is transferred in a refrigerator for refrigeration. Under the condition of not influencing the hatching rate and the growth of the F1 hybrids of hatched silkworm eggs, the gathering data of newly hatched silkworms is delayed by 1 to 25 days so as to be favorable for adjusting the production data of silkworm cocoons, and to reduce the influence of the natural disaster and the temperature anomaly on the growth of mulberry leaves and the influence of the silkworm poisoning and the silkworm disease and other disasters on the growth of the silkworm cocoons, and the method disclosed by the invention is a safe emergency disposal technology for sericulture, and has the hatching rate of more than 95%.
Description
Technical field
The invention belongs to the store method of Eggs of Silkworm, specifically a kind of middle first cross silkworm egg that is suitable for hastening the hatching of silkworms postpones the method for hatching.
Background technology
Silk is the greatness invention of ancient Chinese, and becomes the cultural Symbol that China has world's impact.Planting mulberry and breeding silkworm have history more than 5000 in China, still are at present to use new fresh mulberry leaf to breed silkworms, and this is natural, a precious important symbol of silk product.Based on this technical characterictic; in the now domestic and international silk cocoon large-scale production process; in order to take full advantage of mulberry leaf; also in order effectively to prevent flacherie; sericulture production in various climatic regions all relies on tight plan Silkworm incubation time of cover, and its main points are to make the growth of the growth of silkworm and mulberry tree synchronous; be unlikely to because sericulture causes very much afoliate morning, the reason sericulture is not too late yet causes mulberry leaf to wear out and sericulture later stage and other crops production generation recruitment and control of insect medication contradiction.Yet in recent years, aggravation along with Global climate change, anomalous weather occurs frequent, often because of Low Temperature in Spring or high temperature, and the Changes in weather such as emergent late frost and freeze injury, cause in the Silkworm incubation process, occurring the ANOMALOUS VARIATIONS of mulberry leaf growth, or even mulberry leaf growths destroyed, and has to change the time started of breeding silkworms, if can not in time adjust the time started of breeding silkworms, then cause the waste of serious afoliate or silkworm egg, affect the sericulture income.
At present, cold storage of silkworm egg suppresses technology, is after utilizing the silkworm seed termination of diapause, and body early embryo (before the third embryo of silkworms) has stronger tolerance to low temperature, regulates the production time.But silkworm egg is in case from freezer out, after (be the word of sericulture specialty hasten the hatching of silkworms processing) processed in the hatching that begins to heat, because developmental embryo is poor to the resistance of low temperature environment, even run into anomalous weather, need to postpone and collect ants the time, existing technical measures also can only be regulated 3-5 days time, and cold preservation time is long dead germ to be occurred easily and hatch bad.Go back more effective, the safer method of neither one in the silk cocoon production and suppress the silkworm seed embryonic development, postpone brooding time more than 10 days, this has greatly affected the safety of breeding silkworms and producing.
On the other hand, between the silkworm feeding period, the large and small silkworm death by accident because of pesticide poisoning, flacherie or other reasons all can occur in various places, and will cause the silkworm raiser that go wrong lose receipts as providing without the silkworm egg that hastened the hatching of silkworms timely this moment.Simultaneously, because can't regulating brooding time, raising the rich and influential family can not be according to the actual conditions of self, and reasonable arrangement is raised quantity, reach should take full advantage of favourable season, mulberry leaf are bred silkworms more, avoid again the purpose of labour, apparatus contradiction.Some areas will be carried out the silkworm rearing season layout and be adjusted as increasing by two spring raising etc., often because not providing suitable silkworm egg supply to implement.
For this reason, invent a kind of do not affect the silkworm incubation rate with grow, the hasten the hatching of silkworms method of middle egg-incubation time of flexible modulation, for effective reduction natural calamity and thermic anomaly on the mulberry leaf affects on the growth and silkworm poisons and the disaster such as flacherie on the impact of silk cocoon production, the balance, raising sericulture productivity effect and the sericulture safety that improve sericulture quantity and mulberry leaf output have important value, also provide an effective way for availability, the flexible modulation silkworm rearing season layout that improves the first cross silkworm egg simultaneously.
Summary of the invention
Main task of the present invention is to provide a kind of middle first cross silkworm egg that hastens the hatching of silkworms to postpone the method for hatching; a kind of first cross silkworm egg to having hastened the hatching of silkworms specifically; in low temperature preservation process; by adopting three sections different temperatures protections; the control silkworm seed is grown; prolong the silkworm egg holding time, the technology of flexible modulation egg-incubation time.
In order to solve above technical problem, a kind of middle first cross silkworm egg that hastens the hatching of silkworms of the present invention postpones the method for hatching, its innovative point is as follows: the middle first cross silkworm egg that will hasten the hatching of silkworms carries out the three-sectional low temperature protection, step is as follows: the first step: the first cross silkworm egg in hastening the hatching of silkworms, the silkworm seed embryonic development is behind body pigmentation stage, with 25 ℃, humidity 85 % protect 6-8 hour at former environment in continuation;
Second step: with the above-mentioned first cross silkworm egg that has placed in the silkworm seed box, per 10 silkworm seed boxes are a unit, and external application ash newspaper is wrapped the well-balanced tiling of silkworm egg in the silkworm seed box of each unit; Per two unit are a unit, put into the layer Black cloth bag of wringing out after the immersion, and changing bag put into cover that the sheet lead case closely covers, the dark preservation, put at most 2 of changing bags in each case;
The 3rd step: the sheet lead case is put into 13-15 ℃ of environment insulation 3-4 hour;
The 4th step: above-mentioned sheet lead case changed in 0.0 ℃ ± 0.5 ℃ the freezer, dark surrounds protection 1---5 days, rear adjusting storehouse temperature to 2.5 ± 0.5 ℃, continue dark surrounds protection 6---after 15 days, again temperature is transferred to 5 ℃ ± 0.5 ℃, dark surrounds protection 16---25 days;
The 5th step: with the first cross silkworm egg in the step 4, take out in the section at any time, and through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, the answer later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and processed 1-2 days, and rear sensitization (illumination is higher than 30LUX) impels egg-incubation.
Further, in described the 5th step, the 1-5 that preserves is in day, in the sheet lead case, take out at any time, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and processed 2 days, and rear sensitization (illumination is higher than 30LUX) impels egg-incubation.
Further, in described the 5th step, the 6-15 that preserves is in day, in the sheet lead case, take out at any time, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and processed 1 day, and rear sensitization (illumination is higher than 30LUX) impels egg-incubation.
Further; in described the 5th step; at the 16-25 that preserves in day; the first generation cross-fertilize seed of taking out in the freezer at dusk, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag; reply 25 ℃; the dark surrounds protection of humidity 80-90%, sensitization the next morning (illumination is higher than 30LUX) impels egg-incubation.
The invention has the advantages that: 1, use the inventive method can be in the situation that does not affect the practical incubation rate of first cross silkworm egg silkworm and grow, postponement was collected ants date 1-25 days, be conducive to regulate the silk cocoon date of manufacture, reduce the impact that natural calamity and thermic anomaly are produced silk cocoon disasters such as mulberry leaf affects on the growth and silkworm poisoning and flacheries, improving the balance of sericulture quantity and mulberry leaf output, is the emergency disposal technology of a safety of sericulture production.
2, its innovative point is to have changed in the industry and generally believes that the first cross silkworm egg after beginning to hasten the hatching of silkworms can only be postponed the viewpoint of 3-5 days Silkworm incubation time by supplementary means.Hastening the hatching of silkworms middle silkworm seed embryonic development to body pigmentation stage; by adopting 0.0 ± 0.5 ℃ of deepfreeze; and at the 6th day, the 16th day that refrigerates; improve respectively the three-sectional low temperature guard method of 2.5 ℃ of protection temperature; prolong effective cold preservation time of first cross silkworm egg; the collect ants arrangement of time of control silk cocoon production realizes silkworm rearing season layout and the flexible modulation of silk cocoon date of manufacture.
3, be conducive to the regulation and control sericulture and collect ants the time, regulate kind of a leaf balance; Be conducive to the safety that silk cocoon is produced; Be conducive to reduce the silkworm raiser because of the calamity loss; Be conducive to taking full advantage of of first cross silkworm egg; The emergency disposal that is conducive to the silkworm and mulberry accident.
4, the incubation rate of above-mentioned silkworm first cross silkworm egg reaches more than 95%.
Embodiment
Embodiment 1
Perfectly sound village, evergreen town, Rugao City, Jiangsu in 2010, north latitude 32.23 east longitudes 120.33 have a low-lying mulberry field, 56 mu of areas, because of 4.29 days late frost impact, the mulberry tree young sprout suffered freeze injury, the growing point blackout, blade is dried-up, and growing of mulberry is stagnated, and ordered 65 silkworm eggs of 38 family silkworm raisers this moment outbound begin to hasten the hatching of silkworms, in order to reduce silkworm raiser's loss, prevent from causing the silkworm egg waste, we adopt following methods refrigeration silkworm egg, prolong and hasten the hatching of silkworms the time:
1. the first cross silkworm egg (Su Zhen * spring scenery) in will hastening the hatching of silkworms, silkworm seed embryonic development are behind body pigmentation stage, and with 25 ℃, humidity 85 % protect 6-8 hour at former environment in continuation;
Above-mentioned first cross silkworm egg is good with per 10 the silkworm seed box bundles ofs of plastic ties 2., silkworm egg shakes up tiling in the silkworm seed box, external application ash newspaper is wrapped, put into the layer Black cloth bag of wringing out after the immersion, keep flat silkworm seed box 2 in each bag and prick, and the changing bag that will wrap up silkworm seed box put into the 40cm * 50cm of industry universal, high 40cm cover the sheet lead case;
3. the sheet lead case is put into 13-15 ℃ of environment protection 3-4 hour;
4. above-mentioned sheet lead case is changed in 0.0 ℃ ± 0.5 ℃ the freezer, dark surrounds protection 5 days, rear adjusting storehouse temperature to 2.5 ± 0.5 ℃ continues dark surrounds protection 5 days;
5. the first cross silkworm egg of in freezer, preserving, specific as follows: as in the sheet lead case, to take out silkworm egg, through 13-15 ℃ medium temperature 3-4 hour, the answer later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, humidity 80-90% dark surrounds, remove changing bag, spread out kind, mend to hasten the hatching of silkworms and processed 1 day, rear sensitization (illumination is higher than 30LUX) impels egg-incubation;
6. adopt above method protection, the practical incubation rate of first cross silkworm egg is more than 95%;
Embodiment 2
Between autumn silkworm rearing season, Yang Zhuan village, Dong Chen town, Rugao, Jiangsu, minister village cause 545 silkworm eggs of two villages, 286 family silkworm raisers silkworm in 3 age to be poisoned to death because of paddy rice large tracts of land misuse chlopyrifos in 2009.Adopt the method for embodiment 1 in time low-temperature protection to be suppressed 545 first cross silkworm eggs of 15 days this moment, reissue to the silkworm raiser and raise, and avoided more than 80 ten thousand yuan of silkworm raisers' loss.
。
Embodiment 3
In recent years; the impact of silkworm and mulberry being produced for reducing various factors; each silkworm rearing season; Rugao City, Jiangsu Province is all when the silkworm egg outbound; go out to account in advance silkworm egg and provide the first cross silkworm egg of total amount 10%; adopt the method to make low-temperature protection in the later stage of hastening the hatching of silkworms, postpone Silkworm incubation, the sericulture loss of effectively having avoided a variety of causes to cause.In the time of at most, a silkworm rearing season, 1508 of the silkworm eggs of altogether this method protection of granting employing.
Silkworm egg in the above table is taken out the rear processing of hastening the hatching of silkworms of doing from refrigerated room, according to the different time periods, have nothing in common with each other, specific as follows:
The 1-5 that preserves takes out in the sheet lead case in day at any time, through 13-15 ℃ medium temperature 3-4 hour, the answer later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, and humidity 80-90% dark surrounds removes changing bag, spread out kind, mend to hasten the hatching of silkworms and processed 2 days, rear sensitization (illumination is higher than 30LUX) impels egg-incubation.
The 6-15 that preserves takes out in the sheet lead case in day at any time, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms to process after 1 day and collect ants.
The 16-25 that preserves is in day, and the first generation cross-fertilize seed of taking out in the freezer is at dusk removed changing bag, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, reply 25 ℃, the dark surrounds of humidity 80-90% spreads out kind, and sensitization the next morning (illumination is higher than 30LUX) impels egg-incubation.
Embodiment 4
Xue An town, Rugao, Jiangsu snow bank 30 groups of Xu Ruifeng of neighbourhood committee have 14 mu of mulberry fields, husband and wife's sericultures of being in, and owing to the restriction of the labour of family, apparatus and cocoonery area etc., each issue silkworm can only be raised at most 12 silkworm eggs.Generally speaking, in order to take full advantage of mulberry leaf, silkworm in spring is raised 16-18 and opens, and raises 14 the mid-autumn, raises 12 late fall, the annual sericulture about 42.Because the disposable number of animals raised is large, husband and wife are too busy to do it, and silkworm need to be asked 2 workman's mulberry leaf harvest leaves 5 length of times, add the deficiency of sericulture house, apparatus, open kind of an area during 5 age and put deficiency, cause and nibble the mulberry deficiency, the silkworm body is little, paper cocooning frame is inadequate when being placed on small straw bundles to spin cocoons again, use careless imperial a small bundle of straw, etc. for silkworms to spin cocoons on, cocoon quality is reduced, etc., the silk cocoon per unit area yield of selling is low, and unit price is low again.From spring silkworm rearing season in 2010, by adopting method of the present invention, to collect ants in batches, second batch is postponed than first and being collected ants in 16 days, the large busy period of both having staggered silkworm raising in 5 ages and the harvesting of cocoon of being placed on small straw bundles to spin cocoons, stop the disinfecting time of large silkworm room, tool, prolong again mulberry leaf vegetative period, improve mulberry yield, improve sericulture quantity, increased cocoon yield and benefit, reduced and asked worker's cost, achieved many things at one stroke.Details see Table:
As seen from the above table, the casual household many to the sericulture amount can pass through the method, rationally adjusts feeding time, and the cultivation of staggering reduces labour intensity.Directly reduce aquaculture cost.
Claims (1)
1. the middle first cross silkworm egg of hastening the hatching of silkworms postpones the method for hatching, and it is characterized in that: the middle first cross silkworm egg that will hasten the hatching of silkworms carries out the three-sectional low temperature protection, and step is as follows:
The first step: the first cross silkworm egg in hastening the hatching of silkworms, silkworm seed embryonic development are behind body pigmentation stage, and with 25 ℃, humidity 85 % protect 6-8 hour at former environment in continuation;
Second step: be a unit with per 10 silkworm seed boxes of above-mentioned first cross silkworm egg, external application ash newspaper is wrapped the well-balanced tiling of silkworm egg in the silkworm seed box of each unit; Per two unit are a unit, put into the layer Black cloth bag of wringing out after the immersion, and changing bag put into cover that the sheet lead case closely covers, the dark preservation, put at most 2 of changing bags in each case;
The 3rd step: the sheet lead case is put into 13-15 ℃ of environment insulation 3-4 hour;
The 4th step: above-mentioned sheet lead case changed in 0.0 ℃ ± 0.5 ℃ the freezer, dark surrounds protection 1---5 days, rear adjusting storehouse temperature to 2.5 ± 0.5 ℃, continue dark surrounds protection 6---after 15 days, again temperature is transferred to 5 ℃ ± 0.5 ℃, dark surrounds protection 16---25 days;
The 5th step: with the first cross silkworm egg in the step 4, the 1-5 that preserves at silkworm egg is in day, in the sheet lead case, take out at any time, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, replying the later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and processed 2 days, rear sensitization, illumination is higher than 30LUX, impels egg-incubation; The 6-15 that preserves at silkworm egg is in day, in the sheet lead case, take out at any time, through 13-15 ℃ medium temperature 3-4 hour, remove changing bag, the answer later stage environment that hastens the hatching of silkworms is 25 ℃ of temperature, and humidity 80-90% dark surrounds spreads out kind, mends to hasten the hatching of silkworms and process after 1 day sensitization, illumination is higher than 30LUX, impels egg-incubation; In day, from freezer in take out first generation cross-fertilize seed at dusk at the 16-25 that preserves, through 13-15 ℃ medium temperature 3-4 hour; remove changing bag, reply 25 ℃, the dark surrounds protection of humidity 80-90%; sensitization the next morning, illumination is higher than 30LUX, impels egg-incubation.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104509496B (en) * | 2014-11-28 | 2016-09-28 | 太和县金秋农民养殖专业合作社 | A kind of cultural method of silkworm |
| CN105941347B (en) * | 2016-05-16 | 2018-12-04 | 南京电狐网络科技有限公司 | A method of it is bred silkworms and is cocoond using a rectangular small bundle of straw |
| CN110754442B (en) * | 2019-08-06 | 2021-10-22 | 德清县莫干天竺蚕种有限责任公司 | Silkworm embryo low-temperature protection and seed-delay technology |
| CN112106738A (en) * | 2020-09-11 | 2020-12-22 | 河南省蚕业科学研究院 | Method for nursing one or two-release autumn tussah to relieve diapause |
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| CN102232372A (en) * | 2011-04-28 | 2011-11-09 | 梁文荣 | Equipment and method for supplementary incubation of spring silkworm eggs |
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| JPS5163281A (en) * | 1974-11-22 | 1976-06-01 | Nihon Nosan Kogyo | Sanshunosaiseiho oyobi kaikonoshiikuhoho |
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| CN102232372A (en) * | 2011-04-28 | 2011-11-09 | 梁文荣 | Equipment and method for supplementary incubation of spring silkworm eggs |
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