CN104823927A - Method for preserving plusia agnata staudinger eggs - Google Patents
Method for preserving plusia agnata staudinger eggs Download PDFInfo
- Publication number
- CN104823927A CN104823927A CN201510299416.2A CN201510299416A CN104823927A CN 104823927 A CN104823927 A CN 104823927A CN 201510299416 A CN201510299416 A CN 201510299416A CN 104823927 A CN104823927 A CN 104823927A
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- CN
- China
- Prior art keywords
- container
- eggs
- plusia
- temperature
- ovum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241001367013 Ctenoplusia agnata Species 0.000 title claims abstract description 16
- 235000013601 eggs Nutrition 0.000 title abstract 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000007789 gas Substances 0.000 claims abstract description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 23
- 108010000912 Egg Proteins Proteins 0.000 claims description 23
- 210000004681 ovum Anatomy 0.000 claims description 23
- 239000006004 Quartz sand Substances 0.000 claims description 15
- 238000004321 preservation Methods 0.000 claims description 11
- 238000005057 refrigeration Methods 0.000 claims description 10
- 238000010792 warming Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 6
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims description 2
- 241000238631 Hexapoda Species 0.000 abstract description 5
- 230000003716 rejuvenation Effects 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract 2
- 230000003247 decreasing effect Effects 0.000 abstract 2
- 239000004576 sand Substances 0.000 abstract 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 abstract 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract 1
- 239000001569 carbon dioxide Substances 0.000 abstract 1
- 230000001276 controlling effect Effects 0.000 abstract 1
- 230000012447 hatching Effects 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 229910052710 silicon Inorganic materials 0.000 abstract 1
- 239000010703 silicon Substances 0.000 abstract 1
- 239000000377 silicon dioxide Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 2
- 241000219193 Brassicaceae Species 0.000 description 1
- 241001046947 Ectropis obliqua Species 0.000 description 1
- 241000256259 Noctuidae Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a method for preserving plusia agnata staudinger eggs, and belongs to the technical field of insect breeding. Produced eggs within 24 hours are taken, placed into a closed container and are buried by silica sand, the container is filled with mixed gas of nitrogen, oxygen and carbon dioxide, and the container is closed. The temperature of the container is decreased to range from 2 DEGC to 4 DEG C at the speed ranging from 4 DEG C/h to 5 DEG C/h, and refrigerating is carried out. The temperature of the container is increased to range from 6 DEG C to 8 DEG C at the speed ranging from 8 DEG C/h to 1.2 DEG C/h, and preserving gas is removed and placed in the air. The temperature of the container is continuously increased to range from 10 DEG C to 12 DEG C at the speed ranging from 8 DEG C/h to 1.2 DEG C/h, and the silicon sand is removed. The temperature of the container is continuously increased to the normal temperature at the speed ranging from 8 DEG C/h to 1.2 DEG C/h, and the humidity is maintained to range from 70% to 80%, and normal hatching can be carried out. According to the method for preserving the plusia agnata staudinger eggs, under the low temperature ranging from 2 DEG C to 4 DEG C, the refrigerating time of the plusia agnata staudinger eggs can be increased to 120 days, the rejuvenation probability in the artificial breeding process is greatly decreased, the refrigerated eggs can be hatched according to needs, and the producing, regulating and controlling capacity is greatly improved.
Description
Technical field
The invention belongs to cultured insect technical field.
Background technology
Three-spotted plusia is the Lepidoptera noctuid of a kind of extensive harm Cruciferae, composite family, legume, three-spotted plusia is also a kind of economical insect having certain cultivation and be worth simultaneously, but through too much for after artificial propagation, three-spotted plusia there will be the trend of Quality Down, just needs to introduce wild stocks and carry out rejuvenation work in time there is this situation.Because the three-spotted plusia breeding cycle is short, this rejuvenation process becomes the undesirable element that restriction large area is produced, and due to the particularity of its potential age deduction and feeding process, conservation and hatch as required and just become a technical barrier." the three-spotted plusia artificial diet technique research " delivered as far back as 1994 Nian Liu armies etc. has just set forth the brooding time that can postpone ovum to the refrigeration of three-spotted plusia ovum, pupa, thinks that the refrigeration optimum temperature of ovum is in 8 DEG C, about about 16 days the longest refrigeration phase.After this this technology has no more deep research for many years.Application number be 201310514825.0 Chinese patent document disclose collection and the store method of ovum in a kind of tea geometrid mass rearing, wherein also mention refrigeration looper ovum, but its method is closer to the naturally degeneration-resistant effect utilizing insect ovum, and its cold preservation time is also only 20 days.Though looper and three-spotted plusia belong to lepidopterous insects together in addition, they still have certain difference in distribution with life habit.Most importantly, the worm's ovum cold preservation time of above-mentioned technology is too short, can not meet need of production.
Summary of the invention
The object of the invention is to the shortcoming overcoming prior art, provide a kind of method of preservation three-spotted plusia ovum, the method can extend the cold preservation time of three-spotted plusia ovum greatly.
The inventive method is according to the following steps:
1, refrigerate
Be not later than the ovum of 24 hours after getting output, being placed in can closed container, and use quartz sand landfill, quartz sand landfill thickness was not for have worm's ovum 0.5 ~ 2mm, and quartz sand contains the moisture that mass ratio is 1% ~ 5%; In container, be filled with nitrogen, oxygen, carbon dioxide gas mixture, three's volumetric mixture ratio is 80:14 ~ 16:4 ~ 6, closed container; With the speed of 4 ~ 5 DEG C/h, container is cooled to 2 ~ 4 DEG C, refrigeration;
2, recover
With the speed of 0.8 ~ 1.2 DEG C/h, said vesse is warming up to 6 ~ 8 DEG C, discard preservation gas, be positioned in air, continue to be warming up to 10 ~ 12 DEG C with 0.8 ~ 1.2 DEG C/h speed, discard quartz sand, continue to be warming up to normal temperature with 0.8 ~ 1.2 DEG C/h speed, humidity remains 70% ~ 80%, gets final product normal incubation.
The inventive method worm's ovum can refrigerate 30 ~ 120 days.
Good effect of the present invention: the cold preservation time of three-spotted plusia ovum can be increased to 120 days under the low temperature of 2 ~ 4 DEG C, greatly reduce the probability of rejuvenation in artificial propagation process; The ovum of refrigeration can be hatched as required, substantially increases production ability of regulation and control.
Embodiment
Example 1: see following serial contrast experiment.
In contrast experiment, before refrigeration, process is: get the ovum within 20 hours after output, and being placed in can closed container, and use quartz sand landfill, quartz sand landfill thickness was not for have worm's ovum 1.2mm, and quartz sand contains the moisture that mass ratio is 3%; Nitrogen is filled with, oxygen, carbon dioxide gas mixture, three's volumetric mixture ratio 80:15:5, closed container in container.
This technology in described refrigeration is: with the speed of 4 ~ 5 DEG C/h, container is cooled to 2 DEG C or 4 DEG C of refrigerations.
In contrast experiment, this technical finesse in described recovery mode is: with the speed of 1.0 DEG C/h, said vesse is warming up to 7 DEG C, discard preservation gas, be positioned in air, continue to be warming up to 11 DEG C with 1 DEG C/h speed, discard quartz sand, continue to be warming up to normal temperature with 1 DEG C/h speed, humidity remains 74% ~ 78%.
Following table shows cold storage effect and difference
Example 2: see following experiment, for insufflation gas under the same terms is to the Contrast on effect of ovum preservation.
Get the ovum within 24 hours after output, being placed in can closed container, and use quartz sand landfill, quartz sand landfill thickness was not for have worm's ovum 0.7mm, and quartz sand contains the moisture that mass ratio is 2%; In container, be filled with gas with various (specifically seeing the following form), closed container, with the speed of 4 ~ 5 DEG C/h, container be cooled to 4 DEG C, refrigerate 60 days.
Claims (1)
1. a method for preservation three-spotted plusia ovum, is characterized in that according to the following steps:
Be not later than the ovum of 24 hours after getting output, being placed in can closed container, and use quartz sand landfill, quartz sand landfill thickness was not for have worm's ovum 0.5 ~ 2mm, and quartz sand contains the moisture that mass ratio is 1% ~ 5%; In container, be filled with nitrogen, oxygen, carbon dioxide gas mixture, three's volumetric mixture ratio is 80:14 ~ 16:4 ~ 6, closed container; With the speed of 4 ~ 5 DEG C/h, container is cooled to 2 ~ 4 DEG C, refrigeration;
With the speed of 0.8 ~ 1.2 DEG C/h, said vesse is warming up to 6 ~ 8 DEG C, discard preservation gas, be positioned in air, continue to be warming up to 10 ~ 12 DEG C with 0.8 ~ 1.2 DEG C/h speed, discard quartz sand, continue to be warming up to normal temperature with 0.8 ~ 1.2 DEG C/h speed, humidity remains 70% ~ 80%, gets final product normal incubation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510299416.2A CN104823927B (en) | 2015-06-04 | 2015-06-04 | Method for preserving plusia agnata staudinger eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510299416.2A CN104823927B (en) | 2015-06-04 | 2015-06-04 | Method for preserving plusia agnata staudinger eggs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104823927A true CN104823927A (en) | 2015-08-12 |
| CN104823927B CN104823927B (en) | 2017-01-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510299416.2A Expired - Fee Related CN104823927B (en) | 2015-06-04 | 2015-06-04 | Method for preserving plusia agnata staudinger eggs |
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| CN (1) | CN104823927B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379685A (en) * | 2015-11-25 | 2016-03-09 | 云南农业大学 | Method for preserving exorista japonica townsend pupae |
| CN105638580A (en) * | 2016-02-26 | 2016-06-08 | 珠海友木友科技有限公司 | Refrigeration preservation method for hermetia illucens prepupa |
| CN105724365A (en) * | 2016-02-26 | 2016-07-06 | 珠海友木友科技有限公司 | Black soldier fly egg refrigeration preservation method |
| CN105794723A (en) * | 2016-03-10 | 2016-07-27 | 珠海友木友科技有限公司 | Pupation method for black soldier fly larvae |
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| KR20110005356A (en) * | 2009-07-10 | 2011-01-18 | 안동대학교 산학협력단 | A short-term storage technique of an egg parasitoid, trichogramma sp. using developmental quiescence induced by low temperature |
| CN103960201A (en) * | 2014-05-12 | 2014-08-06 | 山西省农业科学院农产品质量安全与检测研究所 | Plutella xylostella indoor reproduction method |
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| CN104396889A (en) * | 2014-10-27 | 2015-03-11 | 四川农业大学 | Neoseiulus californicus low-temperature storage method |
-
2015
- 2015-06-04 CN CN201510299416.2A patent/CN104823927B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20110005356A (en) * | 2009-07-10 | 2011-01-18 | 안동대학교 산학협력단 | A short-term storage technique of an egg parasitoid, trichogramma sp. using developmental quiescence induced by low temperature |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379685A (en) * | 2015-11-25 | 2016-03-09 | 云南农业大学 | Method for preserving exorista japonica townsend pupae |
| CN105379685B (en) * | 2015-11-25 | 2017-10-10 | 云南农业大学 | A kind of method of the Japanese Exorista civilis pupa of preservation |
| CN105638580A (en) * | 2016-02-26 | 2016-06-08 | 珠海友木友科技有限公司 | Refrigeration preservation method for hermetia illucens prepupa |
| CN105724365A (en) * | 2016-02-26 | 2016-07-06 | 珠海友木友科技有限公司 | Black soldier fly egg refrigeration preservation method |
| CN105794723A (en) * | 2016-03-10 | 2016-07-27 | 珠海友木友科技有限公司 | Pupation method for black soldier fly larvae |
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| CN104823927B (en) | 2017-01-25 |
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Granted publication date: 20170125 Termination date: 20170604 |