CN104823927B - Method for preserving plusia agnata staudinger eggs - Google Patents
Method for preserving plusia agnata staudinger eggs Download PDFInfo
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- CN104823927B CN104823927B CN201510299416.2A CN201510299416A CN104823927B CN 104823927 B CN104823927 B CN 104823927B CN 201510299416 A CN201510299416 A CN 201510299416A CN 104823927 B CN104823927 B CN 104823927B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention provides a method for preserving plusia agnata staudinger eggs, and belongs to the technical field of insect breeding. Produced eggs within 24 hours are taken, placed into a closed container and are buried by silica sand, the container is filled with mixed gas of nitrogen, oxygen and carbon dioxide, and the container is closed. The temperature of the container is decreased to range from 2 DEGC to 4 DEG C at the speed ranging from 4 DEG C/h to 5 DEG C/h, and refrigerating is carried out. The temperature of the container is increased to range from 6 DEG C to 8 DEG C at the speed ranging from 8 DEG C/h to 1.2 DEG C/h, and preserving gas is removed and placed in the air. The temperature of the container is continuously increased to range from 10 DEG C to 12 DEG C at the speed ranging from 8 DEG C/h to 1.2 DEG C/h, and the silicon sand is removed. The temperature of the container is continuously increased to the normal temperature at the speed ranging from 8 DEG C/h to 1.2 DEG C/h, and the humidity is maintained to range from 70% to 80%, and normal hatching can be carried out. According to the method for preserving the plusia agnata staudinger eggs, under the low temperature ranging from 2 DEG C to 4 DEG C, the refrigerating time of the plusia agnata staudinger eggs can be increased to 120 days, the rejuvenation probability in the artificial breeding process is greatly decreased, the refrigerated eggs can be hatched according to needs, and the producing, regulating and controlling capacity is greatly improved.
Description
Technical field
The invention belongs to cultured insect technical field.
Background technology
Three-spotted plusia is a kind of extensive harm Cruciferae, Compositae, the Lepidoptera noctuid of legume, silver simultaneously
Autographa spp is also a kind of economical insecticide with certain cultivation value, but after excessively for artificial propagation, three-spotted plusia can go out
The trend of existing Quality Down, is accomplished by introduction wild stockss when there is this situation and carries out rejuvenation work.Due to crazing
The noctuid breeding cycle is short, and this rejuvenation process becomes the undesirable element that restriction large area produces, and due to its potential warp
Ji be worth and feeding process particularity, conservation and hatch on demand and just become a technical barrier.Send out early in 1994 Nian Liu armies etc.
When " the three-spotted plusia artificial diet technique research " of table just elaborates the hatching that the cold preservation of three-spotted plusia ovum, pupa can be postponed with ovum
Between it is believed that the cold preservation optimum temperature of ovum is in 8 DEG C, the longest about 16 days about the cold preservation phase.Hereafter this technology have no for many years deeper into
Research.The Chinese patent document of Application No. 201310514825.0 discloses a kind of collection of ovum in tea geometrid mass rearing
With store method, wherein also mention cold preservation looper ovum, but its method is closer to the naturally degeneration-resistant effect using insecticide ovum, it is cold
The Tibetan time is also only 20 days.Though other looper and three-spotted plusia belong to lepidopteran insects together, they are in distribution with life habit
Still there is certain difference.Most importantly, the worm's ovum cold preservation time of above-mentioned technology is too short produces needs it is impossible to meet.
Content of the invention
It is an object of the invention to overcoming the shortcoming of prior art, provide a kind of method of preservation three-spotted plusia ovum, the party
Method can greatly prolong the cold preservation time of three-spotted plusia ovum.
The inventive method is according to the following steps:
1st, cold preservation
It is not later than the ovum of 24 hours, being placed in can be in hermetic container, with quartz sand landfill, and quartz sand fills thickness after taking output
For not having worm's ovum 0.5～2mm, quartz sand contains the moisture that mass ratio is 1%～5%；It is filled with nitrogen, oxygen, titanium dioxide toward in container
Carbon mixed gas, three's volumetric mixture ratio is 80:14～16:4～6, hermetic container；With the speed of 4～5 DEG C/h, container is lowered the temperature
To 2～4 DEG C, cold preservation；
2nd, recover
With the speed of 0.8～1.2 DEG C/h, said vesse is warming up to 6～8 DEG C, discards preservation gas, be positioned in the air,
It is continuously heating to 10～12 DEG C with 0.8～1.2 DEG C/h speed, discards quartz sand, continue to be warming up to 0.8～1.2 DEG C/h speed
Room temperature, humidity remains 70%～80%, you can normal incubation.
The inventive method worm's ovum can cold preservation 30～120 days.
The positive effect of the present invention: the cold preservation time of three-spotted plusia ovum can be increased to 120 days in a low temperature of 2～4 DEG C,
Greatly reduce the probability of rejuvenation during artificial propagation；The ovum of cold preservation can be hatched on demand, substantially increases production regulation and control energy
Power.
Specific embodiment
Example 1: see following series contrast experiment.
In contrast experiment, before cold preservation, process is: takes the ovum within 20 hours after output, is placed in and can use in hermetic container
Quartz sand fills, and quartz sand landfill thickness is not have worm's ovum 1.2mm, and quartz sand contains the moisture that mass ratio is 3%；Toward in container
It is filled with nitrogen, oxygen, carbon dioxide gas mixture, three volumetric mixture ratio 80:15:5, hermetic container.
This technology in described cold preservation is: with the speed of 4～5 DEG C/h, container is cooled to 2 DEG C or 4 DEG C of cold preservations.
In contrast experiment, this technology in described recovery mode is processed as: with the speed of 1.0 DEG C/h, said vesse is heated up
To 7 DEG C, discard preservation gas, be positioned in the air, be continuously heating to 11 DEG C with 1 DEG C/h speed, discard quartz sand, continue with 1
DEG C/h speed is warming up to room temperature, humidity remains 74%～78%.
Following table shows cold storage effect and difference
Example 2: see following experiment, be the Contrast on effect to ovum preservation for the insufflation gas under the same terms.
Take the ovum within 24 hours after output, being placed in can be in hermetic container, with quartz sand landfill, and quartz sand landfill thickness is
Do not had worm's ovum 0.7mm, quartz sand contains the moisture that mass ratio is 2%；It is filled with gas with various (specifically see table) toward in container, close
Close container, with the speed of 4～5 DEG C/h, container is cooled to 4 DEG C, cold preservation 60 days.
Claims (1)
1. a kind of method of preservation three-spotted plusia ovum is it is characterised in that according to the following steps:
It is not later than the ovum of 24 hours, being placed in can be in hermetic container, and with quartz sand landfill, quartz sand landfill thickness is not after taking output
Cross worm's ovum 0.5～2mm, quartz sand contains the moisture that mass ratio is 1%～5%；It is filled with nitrogen, oxygen toward in container, carbon dioxide mixes
Close gas, three's volumetric mixture ratio is 80:14～16:4～6, hermetic container；With the speed of 4～5 DEG C/h, container is cooled to 2
～4 DEG C, cold preservation；
With the speed of 0.8～1.2 DEG C/h, said vesse is warming up to 6～8 DEG C, discards preservation gas, be positioned in the air, with
0.8～1.2 DEG C/h speed is continuously heating to 10～12 DEG C, discards quartz sand, continues to be warming up to often with 0.8～1.2 DEG C/h speed
Temperature, humidity remains 70%～80%, you can normal incubation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510299416.2A CN104823927B (en) | 2015-06-04 | 2015-06-04 | Method for preserving plusia agnata staudinger eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510299416.2A CN104823927B (en) | 2015-06-04 | 2015-06-04 | Method for preserving plusia agnata staudinger eggs |
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| CN104823927A CN104823927A (en) | 2015-08-12 |
| CN104823927B true CN104823927B (en) | 2017-01-25 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379685B (en) * | 2015-11-25 | 2017-10-10 | 云南农业大学 | A kind of method of the Japanese Exorista civilis pupa of preservation |
| CN105724365A (en) * | 2016-02-26 | 2016-07-06 | 珠海友木友科技有限公司 | Black soldier fly egg refrigeration preservation method |
| CN105638580A (en) * | 2016-02-26 | 2016-06-08 | 珠海友木友科技有限公司 | Refrigeration preservation method for hermetia illucens prepupa |
| CN105794723A (en) * | 2016-03-10 | 2016-07-27 | 珠海友木友科技有限公司 | Pupation method for black soldier fly larvae |
Citations (5)
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| KR20110005356A (en) * | 2009-07-10 | 2011-01-18 | 안동대학교 산학협력단 | A short-term storage technique of an egg parasitoid, trichogramma sp. using developmental quiescence induced by low temperature |
| CN103960201A (en) * | 2014-05-12 | 2014-08-06 | 山西省农业科学院农产品质量安全与检测研究所 | Plutella xylostella indoor reproduction method |
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| CN104145887A (en) * | 2014-08-30 | 2014-11-19 | 潍坊科技学院 | Low-temperature storage method for artificial propagation of harmonia axyridis |
| CN104396889A (en) * | 2014-10-27 | 2015-03-11 | 四川农业大学 | Neoseiulus californicus low-temperature storage method |
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2015
- 2015-06-04 CN CN201510299416.2A patent/CN104823927B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110005356A (en) * | 2009-07-10 | 2011-01-18 | 안동대학교 산학협력단 | A short-term storage technique of an egg parasitoid, trichogramma sp. using developmental quiescence induced by low temperature |
| CN103960201A (en) * | 2014-05-12 | 2014-08-06 | 山西省农业科学院农产品质量安全与检测研究所 | Plutella xylostella indoor reproduction method |
| CN103975903A (en) * | 2014-06-03 | 2014-08-13 | 周利平 | Method for breeding lycaenidae butterflies |
| CN104145887A (en) * | 2014-08-30 | 2014-11-19 | 潍坊科技学院 | Low-temperature storage method for artificial propagation of harmonia axyridis |
| CN104396889A (en) * | 2014-10-27 | 2015-03-11 | 四川农业大学 | Neoseiulus californicus low-temperature storage method |
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| 小菜蛾室内继代饲养技术及冷藏条件优化;朱九生 等;《山西农业科学》;20120720;第40卷(第7期);第759-762页 * |
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| CN104823927A (en) | 2015-08-12 |
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