CN106034734B - A kind of cultural method improving Uricularia polytricha yield - Google Patents

A kind of cultural method improving Uricularia polytricha yield Download PDF

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CN106034734B
CN106034734B CN201610372734.1A CN201610372734A CN106034734B CN 106034734 B CN106034734 B CN 106034734B CN 201610372734 A CN201610372734 A CN 201610372734A CN 106034734 B CN106034734 B CN 106034734B
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canopy
uricularia polytricha
parts
negative
fermentation
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CN106034734A (en
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苏兴东
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Dongning Shanzhen Food Co ltd North Domain Lover
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Xie Yingge
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • C05D3/02Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to field of planting, in particular to a kind of cultural method for improving Uricularia polytricha yield, it is characterised in that: comprising the following specific steps (1) builds canopy;(2) cultivation matrix inoculation and stacking: the inoculation for being inoculated with Uricularia polytricha strain is followed closely and is inoculated in the bacterium bag for being already equipped with cultivation matrix according to triangle disposition, then bacterium bag is stacked layer by layer, reuses fixed frame and the bacterium bag after stacking is fixed in canopy body and under film layer of shading;(3) training orientation.This method can pass through the unique sun-cloudiness shed of design structure, sunlight is allowed to inject from positive shed face, greenhouse roof air temperature is set to be higher than bottom temp, formation temperature is poor in greenhouse, it will be flowed to top according to physical principle greenhouse bottom air, the warm air for forming flowing can promote the growth of fructification, and can be according to the structure in the growing state adjustment greenhouse of fructification, the more conducively growth of fructification improves yield.

Description

A kind of cultural method improving Uricularia polytricha yield
Technical field
The present invention relates to field of planting, in particular to a kind of cultural method for improving Uricularia polytricha yield.
Background technique
Uricularia polytricha is also known as Yellow-back fungus, and color is dark brown, soft, and delicious flavour is full of nutrition, can element can meat or fish, agaric The content of middle iron is extremely abundant, therefore often eats agaric energy blood-nourishing and preserve youthful looks, and it is ruddy to make us skin, radiant, and can prevent and treat iron-deficient Anaemia;The vitamin K that agaric contains can maintain the normal level of internal coagulation factor, prevent bleeding;Colloid in agaric can be The dust that remains in digestion, impurity absorption put together and excrete, so that playing the role of clearing stomach washs intestines, together When also have more significant neutralizing function to the endogenous such as gall stone, kidney stone foreign matter;Also help digest fiber substance function Can, there are dissolution and molten to act on the foreign matters such as the hair for being difficult to digest, husk, wooden slag, sand, the metal fillings eaten unintentionally, It therefore, is mine, chemical industry and the indispensable health food of textile worker.Agaric contains anti-tumor active substance, can enhance machine Body immunity, it is often edible to can prevent cancer.Uricularia polytricha is tender and crisp palatable, like jellyfish, can it is cold and dressed with sauce, fry, quick-fried soup, it is deep to be disappeared Expense person's likes;Its wild Uricularia polytricha is distributed mainly on Heilungkiang, Jilin, Fujian, Taiwan, Hubei, Guangdong, Guangxi, Sichuan, expensive The ground such as state, Yunnan are grown in the rotten wood of more than the 120 kinds of broad leaf trees such as oak, poplar, banyan, Chinese scholartree, Dan Sheng or all living creatures.
And market is constantly increasing the demand of Uricularia polytricha, is cultivateeed at present with Duan Mu's and based on Bag Material, It is able to carry out artificial culture throughout the country, those skilled in the art are also to influence Uricularia polytricha nutrition, quality and yield Factor is studied, and finds different plantation formula material and cultural method to Uricularia polytricha nutrition, quality and yield It influences very big.
Therefore, for a person skilled in the art, need to develop a kind of implantation methods that suitable Uricularia polytricha is grown.
Summary of the invention
In view of above content, it is necessary to provide a kind of cultural method for improving Uricularia polytricha yield, this method can pass through design The sun-cloudiness shed of unique structure allows sunlight to inject from positive shed face, and greenhouse roof air temperature is made to be higher than bottom temp, shape in greenhouse At temperature difference, will be flowed to top according to physical principle greenhouse bottom air, the warm air for forming flowing can promote fructification Growth, and can according to fructification growing state adjustment greenhouse in structure, the more conducively growth of fructification, improve yield.
In order to achieve the above objectives, the technical scheme adopted by the invention is that:
A kind of cultural method improving Uricularia polytricha yield, comprising the following specific steps
(1) build canopy: using the canopy body of several long 8-10m × wide 5-6m × high 6-7m in brackets besieged city, canopy body surrounding is first covered Then lid shading film covers black film, reuse surrounding of the locking bar in canopy body middle and upper part and successively join end to end and consolidate the black film of covering It is fixed, and several support brackets, and support bracket and canopy body side length parallel arrangement are installed in canopy body medium position;The load-bearing Support height is higher than bracket;Yang Peng and negative canopy are formed between support bracket and bracket, Yang Peng and negative canopy are arranged back-to-back, and mutually Connection, the roofing inclination of Yang Peng towards being arranged in the east, and the roofing inclination of negative canopy is arranged towards west, and the ridge of Yang Peng is higher than negative canopy Ridge, be equipped with vent passages between Yang Peng and negative canopy, vent passages are covered with black film, cover on the roofing of Yang Peng and negative canopy There is black film, light-transmissive film layer, foam sheet and shading film layer is from top to bottom disposed in the sun canopy, and light-transmissive film layer is close to The roofing of Yang Peng is from top to bottom disposed with light-transmissive film layer, foam sheet and shading film layer in negative canopy, and light-transmissive film layer is close to The roofing of negative canopy;
(2) cultivation matrix inoculation and stacking: the inoculation for being inoculated with Uricularia polytricha strain is followed closely and is inoculated with according to triangle disposition In in the bacterium bag for being already equipped with cultivation matrix, then bacterium bag is stacked layer by layer, reuses fixed frame and fix the bacterium bag after stacking In in canopy body and under film layer of shading;
(3) it training orientation: is covered with the shading film of canopy body surrounding in Uricularia polytricha culture medium bacterium bag after mycelia daily in morning It is rolled during upper 10 points to 12 points, remaining time puts down covering, until stopping after Uricularia polytricha fruiting;At Uricularia polytricha fruiting 3-8 days The black film covered on the roofing of Yang Peng is rolled afterwards;The foam sheet in positive canopy is dismantled after Uricularia polytricha fruiting 9-16 days;When Uricularia polytricha fruiting is after 10-14 days by the black film of canopy body surrounding covering during 3 points to 5 points of 10 points to 12 points of every morning and afternoon It rolls, remaining time puts down covering, and so circulation is until Uricularia polytricha harvesting finishes.
In the present invention, it further illustrates, further includes the preparation of cultivation matrix in step (2), specifically comprise the following steps:
A feedstock processing: the broken wood shavings of level-one, the broken wood shavings classification of second level are exposed to the sun 2-3 days under the sun;By Shell of Water Chestnut and sesame Stalk distinguishes flour to 1-2cm partial size after being also exposed to the sun under the sun 3-4 days;Value of Spartina Anglica and durian shell are dried to holding nothing Tide sense, is then respectively crushed into 1-2cm partial size;It is 1-2cm that garlic stalk, apple pomace are crushed to partial size respectively;By rape After chopped straw stalk high-temperature sterilization is dry after granular mill stirs, the solid particle that particle diameter is 0.3-0.7cm is obtained;It will Soy sauce waste residue is handled to salt content by the desalinization of soil by flooding or leaching less than 1%;By Klinit dregs through peracid treatment, pH=5-6 is adjusted;
B fermentation processing: according to weight part ratio, by 5-15 parts of the sesame stalk after processing of step A, Shell of Water Chestnut 5-15 Part, 3-10 parts of Value of Spartina Anglica, 3-10 parts of durian shell, 3-10 parts of garlic stalk, 3-10 parts of apple pomace, 5-8 parts of Klinit dregs, level-one are broken 5-8 parts of wood shavings, 5-8 parts of rape flower stalk and 5-8 parts of soy sauce waste residue, with 3-5 parts of wheat bran, 5-10 parts of precipitated calcium carbonate and soybean powder 5-15 parts of mixings, add milk of lime and water, adjust pH to 5-7, control moisture content 60-75%, obtain fermentation material, will Fermentation material and the anti-microbial inoculum of 5-15 parts of trichodermas mix, and build heap fermentation 20-30h, 35-45 DEG C of fermentation temperature, can be obtained;
C charging processing: moisture content is adjusted as 55- using distilled water for broken wood shavings 5-8 parts of second level for treated in step A 70%, it is then charged into culture bag, adjustment second level wood shavings form hollow form and are tightly attached on the outside of the internal layer of culture bag, then again will step Fermentation material after the completion of rapid B fermentation is filled in hollow position, is wrapped in fermentation material in the broken wood shavings of second level, and then sealing obtains bag Fill culture material;
D sterilization treatment: bag cultivating material is sterilized 30-45min at 121 DEG C, is obtained the Uricularia polytricha with diseases prevention and is planted Train matrix.
In the present invention, it further illustrates, the anti-microbial inoculum of trichoderma is that Lightyellow Sophora Root and trichoderma are consolidated microbial inoculum according to quality It is mixed than 1:1, builds heap and ferment 15-20 days;It is to be prepared into hook-shaped trichoderma after inclined-plane culture that the trichoderma, which consolidates microbial inoculum, Its spore concentration is 3 × 108The seed liquor of a spore/mL, then be inoculated with and cultivated as in solid-state fermentation culture medium, it is described solid State fermentation medium is that 0.1g ammonium sulfate, 0.05g potassium dihydrogen phosphate, 0.04g dipotassium hydrogen phosphate is added in 15.0g sucrose waste liquid With agar 15.0g, adjusting water content is 65%, keeps pH naturally, in 131 DEG C of sterilizing 35min.
In the present invention, it further illustrates, length, thickness, the wide size of the broken wood shavings of level-one are 30-60 × 0.4-1.0 ×30-80mm;Length, thickness, the wide size of the broken wood shavings of second level are 3-8 × 0.1-0.15 × 0.15-0.25mm.
In the present invention, it further illustrates, the ridge of the ridge of the Yang Peng and negative canopy is on the same vertical plane, institute The difference in height stated between the ridge of Yang Peng and the ridge of negative canopy is 0.3-0.6m.
In the present invention, it further illustrates, in step (1), pit digging is also carried out in canopy body, and is external lower than canopy after pit digging Ground level 0.2-0.3m.
In the present invention, it further illustrates, the periphery of the canopy body is additionally provided with dewatering ditch.
In the present invention, it further illustrates, the number of plies stacked layer by layer is 8-12 layers.
The invention has the following advantages:
1, method of the invention can be kept in canopy body by the unique sun-cloudiness shed of design structure before Uricularia polytricha fruiting Darkness and temperature, be conducive to inoculation mycelia rapid growth;After Uricularia polytricha fruiting, the black film in positive shed face can be raised, Positive shed face is enabled to inject sunlight, on during the sun rises, and injects, is irradiated at negative canopy first from inclined positive shed face Portion, so that vapor of a whole night transpiration in middle and upper part in canopy body warms, so that the atmospheric density of middle and upper part becomes smaller in negative canopy, so The intracorporal air themperature of canopy lower (density is larger) will flow middle and upper part into negative canopy afterwards, when the air of Yang Peng is to negative canopy middle and upper part When flowing, a part of cold air will be swapped from the vent passages air external with canopy, and according to this, the intracorporal air of canopy will be by Gradual change is warm, can adjust the intracorporal air environment of canopy, humidity and temperature, so that Uricularia polytricha is grown poor round the clock, Neng Gouchang Thick and solid fructification out is quickly grown it is also possible that canopy body possesses ozone and enough oxygen by Uricularia polytricha, with The time postpone, sunlight enters the lower part that negative canopy is successively injected from the middle and upper part of negative canopy and direct projection in positive canopy, so that positive canopy yin canopy Sunlight irradiation is all obtained, prevents from growing bacterium in canopy body, good growing environment is provided to Uricularia polytricha, guarantees the fruiting of fructification Rate;After fructification is to a certain size, the cystosepiment in positive canopy is dismantled, so that the sunlight light transmittance in positive canopy increases, it is full Amount of sunlight needed for sufficient sporophore growth promotes the Uricularia polytricha planted in positive canopy than planting the Uricularia polytricha growth in negative canopy faster, Be staggered the collecting time of Yang Peng and negative canopy Uricularia polytricha, guarantees that the Uricularia polytricha of harvesting can be sold in the case where rising trend is preferably most delicious Out, prevent disposable a large amount of harvesting, supply exceed demand, the Uricularia polytricha of waste can only peasant household voluntarily dry and sell again, the time is long, cost Height, low-cost problem occur.
2, present invention employs inoculation nail triangle disposition inoculation, inoculation nail joint kind is simple to operate, and triangle disposition connects Kind can will grow fructification is staggered growth, opposite by harvesting from both ends fruiting in the way of, is utilizing identical space of the invention Inoculation method yield it is higher, be not only to increase income from space utilization, also from sunlight absorb top grade word be staggered and fructification can be made to inhale Receipts sunlight is identical, and rising trend is uniform, and " one " word is stacked, and enables to the bacterium bag fruiting time on upper layer fast, when the son on harvesting upper layer is real After body, lower layer, which starts largely to absorb sunlight, then to be harvested, and staggering time harvesting can be harvested for the market demand, will be met Market is closed, the loss of marketing failure is avoided.
3, the cultivation matrix that the present invention uses, using wheat bran, soybean powder, broken wood shavings, Value of Spartina Anglica, precipitated calcium carbonate as base-material master Carbon and nitrogen sources are provided, wherein with addition of adventitious plant Value of Spartina Anglica, it be rich in a large amount of crude fat, crude protein, crude fibre, nitrogen-free leaching The nutriments such as object out are capable of providing nutriment required for Uricularia polytricha growth period, additionally provide to the excessive numerous of denizen Grow the solution new way for causing zonal environmental problem;Wheat bran provides cellulose and vitamine B group, and being rich in soybean powder has greatly Protein, fat and the carbohydrate of amount, also having compounded in culture medium in rape flower stalk containing rape element sterol is sterol Parahormone can induce the quick merisis of strain of inoculation, differentiation, so as to shorten the period of fruiting;Sesame stalk, garlic stalk In rich in there is a variety of nutriments such as cellulose, hemicellulose, sugarcane skin contains the substances such as sugar, lignin, cellulose, can provide Carbon nitrogen source needed for edible fungi growth, and sesame stalk, garlic stalk have good gas permeability, are conducive to mycelia growth, water suction Performance is good, can regulate and control the humidity of whole cultivation matrix, the humidity of cultivation matrix is kept to play the growth of mycelia and fructification Critical effect, this also provides a new processing method to agricultural wastes, economic and environment-friendly, and garlic stalk also contains greatly Allicin, can play inhibiting effect to the growth of certain miscellaneous bacterias, and edible mushroom can reduce miscellaneous bacteria infection, improve yield;Durian shell It with addition of the microelements such as a large amount of Ca, K, Fe and a variety of amino acid can be provided, is absorbed and used in sporophore growth, improves son Amino acid content in entity;It is real in mycelia, son not only to provide a large amount of organic nutrient substance also with addition of apple pomace for cultivation matrix Body growth, wherein the acidic materials contained can also neutralize the taste of garlic stalk, durian shell, so that mixed cultivation matrix does not have There is especially pungent taste;Tissue and metabolite, fermentation raw material in soy sauce waste material containing microorganism also contain moisture, thick The ingredients such as albumen, crude fat, reduced sugar, salinity, calcium, phosphorus, ash content, the nutriment not only containing basis, moreover it is possible to make full use of Containing nutriments such as sugar, alcohol needed for edible fungi growth, organic acids in the tissue of microorganism, desalination is carried out to soy sauce waste material Processing guarantees that mixed whole raw material salt content is maintained in the salt content environment for the growth for being conducive to edible mycelia, The period on culture medium is covered with so as to shorten mycelia after inoculation;With addition of Shell of Water Chestnut provide carbon nitrogen source, adjust overall broth Carbon nitrogen source ratio, moreover it is possible to which the growth and growth for inhibiting staphylococcus aureus and Escherichia coli in culture medium provide good food With the growing environment of bacterium, the disease of Uricularia polytricha is reduced, in conclusion the raw material that the present invention uses, complements each other, complement auxotrophic, Can also pre- disease prevention, since Ji Yuan prevent pathogeny, guarantee Uricularia polytricha quality, improve output capacity.
4, cultivation matrix of the invention is also by raw material xylose alcohol slag through peracid treated Klinit dregs its celluloses, half fiber The nutritions Partial digestions such as element, lignin are tieed up, is conducive to mycelia and absorbs, the pH value of mixed entire culture medium is adjusted Effect, is more suitable for Uricularia polytricha fruiting;When packing processing by the way of " second level wood shavings wrap up fermentation material ", first, as hair The effect of ferment material insulating layer after inoculation Uricularia polytricha inoculation bacterium, second, when covering with mycelia and starting fruiting, play certain obstruction Effect, can be improved the quality of the Uricularia polytricha of fruiting, because of one obstruction of setting in fruiting, i.e., by the Uricularia polytricha of fruiting used Property active force under, burst out stronger vigor, from research and test result in known to suitably increase certain obstruction to fruiting The biologos of Uricularia polytricha can be improved.
5, the anti-microbial inoculum of the trichoderma of the application is consolidated microbial inoculum with trichoderma and is used cooperatively using Lightyellow Sophora Root as substrate, and it is anti-to play collaboration The effect of disease, and reduce the usage amount of chemical insecticide, and Lightyellow Sophora Root does not easily lead to nuisance as substrate and generates anti-medicine Property, it can be used for a long time.
Detailed description of the invention
Fig. 1 is the structural schematic diagram inside canopy body of the present invention;
Fig. 2 is the structural schematic diagram of Uricularia polytricha planting greenhouse of the present invention;
Fig. 3 is the structural schematic diagram that Uricularia polytricha bacterium bag of the present invention stacks mode, inoculation nail triangle disposition;
In figure, 1 vent passages, 2 negative canopies, 3 light-transmissive film layers, 4 brackets, 5 foam sheets, 6 shading film layers, 7 maos of wood Ear culture medium bacterium bag, 8 support brackets, 9 Yang Peng, 10 black films, 11 locking bars, 12 gutters, 13 fixed frames, 14 inoculations Nail.
The present invention that the following detailed description will be further explained with reference to the above drawings.
Specific embodiment
The anti-microbial inoculum of trichoderma in following embodiment is that Lightyellow Sophora Root is consolidated microbial inoculum with trichoderma to mix according to mass ratio 1:1, Heap is built to ferment 15-20 days;Wherein, it is that hook-shaped trichoderma is prepared into its spore concentration after inclined-plane culture that trichoderma, which consolidates microbial inoculum, It is 3 × 108The seed liquor of a spore/mL, then be inoculated with and cultivated as in solid-state fermentation culture medium, the solid state fermentation culture Base is that 0.1g ammonium sulfate, 0.05g potassium dihydrogen phosphate, 0.04g dipotassium hydrogen phosphate and agar is added in 15.0g sucrose waste liquid 15.0g, adjustment water content are 65%, keep pH naturally, in 131 DEG C of sterilizing 35min.
Embodiment 1:
A kind of cultural method improving Uricularia polytricha yield, comprising the following specific steps
(1) build canopy: if Fig. 1,2 use the canopy body of several long 8m × wide 6m × high 7m in brackets besieged city, canopy body surrounding is first covered Then lid shading film covers black film, reuse surrounding of the locking bar in canopy body middle and upper part and successively join end to end and consolidate the black film of covering It is fixed, and several support brackets, and support bracket and canopy body side length parallel arrangement are installed in canopy body medium position;Support bracket Height is higher than bracket;Yang Peng and negative canopy are formed between support bracket and bracket, Yang Peng and negative canopy are arranged back-to-back, and mutually interconnect Logical, the roofing inclination of Yang Peng towards being arranged in the east, and the roofing inclination of negative canopy is arranged towards west, and the ridge of Yang Peng is higher than negative canopy The ridge of ridge, the ridge of Yang Peng and negative canopy is on the same vertical plane, the height between the ridge of Yang Peng and the ridge of negative canopy Spending difference is 0.6m, and the difference in height between Yang Peng and negative canopy forms vent passages, and vent passages are covered with black film, Yang Peng and negative canopy It is all covered with black film on roofing, light-transmissive film layer, foam sheet and shading film layer are from top to bottom disposed in the positive canopy of institute, and thoroughly Light film layer is close to the roofing of Yang Peng, light-transmissive film layer, foam sheet and shading film layer is from top to bottom disposed in negative canopy, and thoroughly Light film layer is close to the roofing of negative canopy, is additionally provided with dewatering ditch in the periphery of canopy body, pit digging is also carried out in canopy body, and is low after pit digging In the external ground level 0.3m of canopy;
(2) preparation of cultivation matrix: specifically comprise the following steps:
A feedstock processing: being the broken wood shavings of level-one of 60 × 1.0 × 80mm, length, thickness, wide size by length, thickness, wide size It is exposed to the sun under the sun 3 days for the broken wood shavings classification of second level of 8 × 0.15 × 0.25mm;By Shell of Water Chestnut and sesame stalk also under the sun Flour is distinguished after being exposed to the sun 4 days to 2cm partial size;Value of Spartina Anglica and durian shell are dried to and hold amphidromic sense, is then crushed respectively At to 2cm partial size;It is 2cm that garlic stalk, apple pomace are crushed to partial size respectively;It will be passed through after rape flower straw under high temperature sterilizing-drying After crossing granular mill stirring, the solid particle that particle diameter is 0.7cm is obtained;Soy sauce waste residue is handled by the desalinization of soil by flooding or leaching to saliferous Amount is less than 1%;By Klinit dregs through peracid treatment, pH=6 is adjusted;
B fermentation processing: according to weight part ratio, by 15 parts of the sesame stalk after processing of step A, 15 parts of Shell of Water Chestnut, big 10 parts of spartina, 10 parts of durian shell, 10 parts of garlic stalk, 10 parts of apple pomace, 8 parts of Klinit dregs, 8 parts of the broken wood shavings of level-one, rape flower 8 parts and 8 parts of soy sauce waste residue of stalk, with 5 parts of wheat bran, 10 parts of precipitated calcium carbonate and 15 parts of soybean powder mixings, add lime Cream and water adjust pH to 7, control moisture content 75%, obtain fermentation material, and fermentation material and the anti-microbial inoculum of 15 parts of trichodermas are mixed, built Heap fermentation 30h, can be obtained by 45 DEG C of fermentation temperature;
C charging processing: by treated in step A, 8 parts of the broken wood shavings of second level use distilled water adjusting moisture contents are 70%, It is then charged into culture bag, adjustment second level wood shavings form hollow form and are tightly attached on the outside of the internal layer of culture bag, then again send out step B Fermentation material after the completion of ferment is filled in hollow position, is wrapped in fermentation material in the broken wood shavings of second level, and then sealing obtains packed cultivation Training material;
D sterilization treatment: bag cultivating material is sterilized 45min at 121 DEG C, obtains the Uricularia polytricha cultivation base with diseases prevention Matter;
(3) cultivation matrix inoculation and stacking:: as Fig. 3 inoculation that will be inoculated with Uricularia polytricha strain is followed closely according to " product " word Shape is inoculated in the bacterium bag for being already equipped with cultivation matrix, then bacterium bag is stacked to 12 layers layer by layer, and reusing fixed frame will be after stacking Bacterium bag be fixed in canopy body and under film layer of shading;
(4) it training orientation: is covered with the shading film of canopy body surrounding in Uricularia polytricha culture medium bacterium bag after mycelia daily in morning It is rolled during upper 10 points to 12 points, remaining time puts down covering, until stopping after Uricularia polytricha fruiting;After Uricularia polytricha fruiting 8 days The black film covered on the roofing of Yang Peng is rolled;The foam sheet in positive canopy is dismantled after Uricularia polytricha fruiting 16 days;When hair wood The black film that canopy body surrounding covers is rolled during 3 points to 5 points of 10 points to 12 points of every morning and afternoon after ear fruiting 14 days, The remaining time puts down covering, and so circulation is until Uricularia polytricha harvesting finishes.
Embodiment 2:
A kind of cultural method improving Uricularia polytricha yield, comprising the following specific steps
(1) canopy is built:: if Fig. 1,2 use the canopy body of several long 8m × wide 5m × high 6m in brackets besieged city, canopy body surrounding is first covered Then lid shading film covers black film, reuse surrounding of the locking bar in canopy body middle and upper part and successively join end to end and consolidate the black film of covering It is fixed, and several support brackets, and support bracket and canopy body side length parallel arrangement are installed in canopy body medium position;Support bracket Height is higher than bracket;Yang Peng and negative canopy are formed between support bracket and bracket, Yang Peng and negative canopy are arranged back-to-back, and mutually interconnect Logical, the roofing inclination of Yang Peng towards being arranged in the east, and the roofing inclination of negative canopy is arranged towards west, and the ridge of Yang Peng is higher than negative canopy The ridge of ridge, the ridge of Yang Peng and negative canopy is on the same vertical plane, the height between the ridge of Yang Peng and the ridge of negative canopy Spending difference is 0.3m, and the difference in height between Yang Peng and negative canopy forms vent passages, and vent passages are covered with black film, Yang Peng and negative canopy It is all covered with black film on roofing, light-transmissive film layer, foam sheet and shading film layer, and light transmission are from top to bottom disposed in positive canopy Film layer is close to the roofing of Yang Peng, and light-transmissive film layer, foam sheet and shading film layer, and light transmission are from top to bottom disposed in negative canopy Film layer is close to the roofing of negative canopy, is additionally provided with dewatering ditch in the periphery of canopy body, pit digging is also carried out in canopy body, and be lower than after pit digging The external ground level 0.2m of canopy;
(2) preparation of cultivation matrix: specifically comprise the following steps:
A feedstock processing: being the broken wood shavings of level-one of 60 × 1.0 × 80mm, length, thickness, wide size by length, thickness, wide size It is exposed to the sun under the sun 3 days for the broken wood shavings classification of second level of 8 × 0.15 × 0.25mm;By Shell of Water Chestnut and sesame stalk also under the sun Flour is distinguished after being exposed to the sun 4 days to 2cm partial size;Value of Spartina Anglica and durian shell are dried to and hold amphidromic sense, is then crushed respectively At to 2cm partial size;It is 2cm that garlic stalk, apple pomace are crushed to partial size respectively;It will be passed through after rape flower straw under high temperature sterilizing-drying After crossing granular mill stirring, the solid particle that particle diameter is 0.7cm is obtained;Soy sauce waste residue is handled by the desalinization of soil by flooding or leaching to saliferous Amount is less than 1%;By Klinit dregs through peracid treatment, pH=6 is adjusted;
B fermentation processing: according to weight part ratio, by 5 parts of the sesame stalk after processing of step A, 5 parts of Shell of Water Chestnut, rice Careless 3 parts, 3 parts of durian shell, 3 parts of garlic stalk, 3 parts of apple pomace, 5 parts of Klinit dregs, 5 parts, 5 parts of rape flower stalk of the broken wood shavings of level-one With 5 parts of soy sauce waste residue, with 3 parts of wheat bran, 5 parts of precipitated calcium carbonate and 5 parts of soybean powder mixing, add milk of lime and water, adjust PH to 5 is saved, moisture content 60% is controlled, obtains fermentation material, fermentation material and the anti-microbial inoculum of 5 parts of trichodermas are mixed, builds heap fermentation 20h, It 35 DEG C of fermentation temperature, can be obtained;
C charging processing: by treated in step A, 5 parts of the broken wood shavings of second level use distilled water adjusting moisture contents are 55%, It is then charged into culture bag, adjustment second level wood shavings form hollow form and are tightly attached on the outside of the internal layer of culture bag, then again send out step B Fermentation material after the completion of ferment is filled in hollow position, is wrapped in fermentation material in the broken wood shavings of second level, and then sealing obtains packed cultivation Training material;
D sterilization treatment: bag cultivating material is sterilized 30min at 121 DEG C, obtains the Uricularia polytricha cultivation base with diseases prevention Matter;
(3) cultivation matrix inoculation and stacking:: as Fig. 2 inoculation that will be inoculated with Uricularia polytricha strain is followed closely according to " product " word Shape is inoculated in the bacterium bag for being already equipped with cultivation matrix, then bacterium bag is stacked to 8 layers layer by layer, and reusing fixed frame will be after stacking Bacterium bag is fixed in canopy body and under film layer of shading;
(4) it training orientation: is covered with the shading film of canopy body surrounding in Uricularia polytricha culture medium bacterium bag after mycelia daily in morning It is rolled during upper 10 points to 12 points, remaining time puts down covering, until stopping after Uricularia polytricha fruiting;After Uricularia polytricha fruiting 3 days The black film covered on the roofing of Yang Peng is rolled;The foam sheet in positive canopy is dismantled after Uricularia polytricha fruiting 9 days;Work as Uricularia polytricha The black film that canopy body surrounding covers is rolled during 3 points to 5 points of 10 points to 12 points of every morning and afternoon after fruiting 10 days, remaining Time puts down covering, and so circulation is until Uricularia polytricha harvesting finishes.
Embodiment 3:
A kind of cultural method improving Uricularia polytricha yield, comprising the following specific steps
(1) canopy is built:: as Fig. 1,2 use the canopy body of several long 9m × wide 5.5m × high 6.5m in brackets besieged city, canopy body surrounding Then first covering shading film covers black film, reuse surrounding of the locking bar in canopy body middle and upper part and successively join end to end the black of covering Film is fixed, and is equipped with several support brackets, and support bracket and canopy body side length parallel arrangement in canopy body medium position;Load-bearing Support height is higher than bracket;Yang Peng and negative canopy are formed between support bracket and bracket, Yang Peng and negative canopy are arranged back-to-back, and mutually Connection, the roofing inclination of Yang Peng towards being arranged in the east, and the roofing inclination of negative canopy is arranged towards west, and the ridge of Yang Peng is higher than negative canopy Ridge, the ridge of the ridge of Yang Peng and negative canopy be on the same vertical plane, between the ridge of Yang Peng and the ridge of yin canopy Difference in height is 0.45m, and the difference in height between Yang Peng and negative canopy forms vent passages, and vent passages are covered with black film, Yang Peng and yin It is all covered with black film on the roofing of canopy, light-transmissive film layer, foam sheet and shading film layer are from top to bottom disposed in positive canopy, and Light-transmissive film layer is close to the roofing of Yang Peng, and light-transmissive film layer, foam sheet and shading film layer are from top to bottom disposed in negative canopy, and Light-transmissive film layer is close to the roofing of negative canopy, is additionally provided with dewatering ditch in the periphery of canopy body, pit digging is also carried out in canopy body, and after pit digging The ground level 0.25m external lower than canopy;
(2) preparation of cultivation matrix: specifically comprise the following steps:
A feedstock processing: being the broken wood shavings of level-one of 50 × 0.6 × 55mm, length, thickness, wide size by length, thickness, wide size It is exposed to the sun under the sun 3 days for the broken wood shavings classification of second level of 5 × 0.12 × 0.20mm;By Shell of Water Chestnut and sesame stalk also under the sun Flour is distinguished after being exposed to the sun 3 days to 2cm partial size;Value of Spartina Anglica and durian shell are dried to and hold amphidromic sense, is then crushed respectively At to 1cm partial size;It is 2cm that garlic stalk, apple pomace are crushed to partial size respectively;It will be passed through after rape flower straw under high temperature sterilizing-drying After crossing granular mill stirring, the solid particle that particle diameter is 0.5cm is obtained;Soy sauce waste residue is handled by the desalinization of soil by flooding or leaching to saliferous Amount is less than 1%;By Klinit dregs through peracid treatment, pH=5.5 is adjusted;
B fermentation processing: according to weight part ratio, by 8 parts of the sesame stalk after processing of step A, 10 parts of Shell of Water Chestnut, rice Careless 6 parts, 5 parts of durian shell, 9 parts of garlic stalk, 8 parts of apple pomace, 7 parts of Klinit dregs, 6 parts, 7 parts of rape flower stalk of the broken wood shavings of level-one With 7 parts of soy sauce waste residue, with 4 parts of wheat bran, 8 parts of precipitated calcium carbonate and 12 parts of soybean powder mixing, add milk of lime and water, adjust PH to 6 is saved, moisture content 65% is controlled, obtains fermentation material, fermentation material and the anti-microbial inoculum of 10 parts of trichodermas are mixed, builds heap fermentation 25h, It 40 DEG C of fermentation temperature, can be obtained;
C charging processing: by treated in step A, 7 parts of the broken wood shavings of second level use distilled water adjusting moisture contents are 65%, It is then charged into culture bag, adjustment second level wood shavings form hollow form and are tightly attached on the outside of the internal layer of culture bag, then again send out step B Fermentation material after the completion of ferment is filled in hollow position, is wrapped in fermentation material in the broken wood shavings of second level, and then sealing obtains packed cultivation Training material;
D sterilization treatment: bag cultivating material is sterilized 38min at 121 DEG C, obtains the Uricularia polytricha cultivation base with diseases prevention Matter;
(3) cultivation matrix inoculation and stacking: the inoculation for being inoculated with Uricularia polytricha strain is followed closely and is inoculated with according to triangle disposition In in the bacterium bag for being already equipped with cultivation matrix, then bacterium bag is stacked to 10 layers layer by layer, reuses fixed frame for the bacterium bag after stacking It is fixed in canopy body and under film layer of shading;
(4) it training orientation: is covered with the shading film of canopy body surrounding in Uricularia polytricha culture medium bacterium bag after mycelia daily in morning It is rolled during upper 10 points to 12 points, remaining time puts down covering, until stopping after Uricularia polytricha fruiting;After Uricularia polytricha fruiting 5 days The black film covered on the roofing of Yang Peng is rolled;The foam sheet in positive canopy is dismantled after Uricularia polytricha fruiting 12 days;When hair wood The black film that canopy body surrounding covers is rolled during 3 points to 5 points of 10 points to 12 points of every morning and afternoon after ear fruiting 11 days, The remaining time puts down covering, and so circulation is until Uricularia polytricha harvesting finishes.
Comparative example:
Culture medium prescription are as follows: sawdust 78%, rice bran (or wheat bran) 19%, sugar 1%, gypsum 1%, urea 0.5%, peroxophosphoric acid Calcium 0.5%;It is in 65% according to the conventional control moisture content that mixes, pH value 6.5, sterilize 40min at 131 DEG C, obtains cultivation Train matrix.Cultivation greenhouse is common greenhouse, carries out " well " font and stacks cultivation, regulating environmental humidity 50-70%, temperature 25 Degree Celsius hereinafter, with rear venting 2 hours, keep dark condition until fruiting 4 points of every afternoon, light-exposed rate is kept daily after fruiting It is 3-5 hours daily for 50-60%.
Embodiment 1-3, comparative example are prepared cultivation matrix (the wherein specification of culture bag are as follows: 17cm × 33cm × 0.005cm, every 30 bags of formula, if 3 repetitions, every sacked material closes dry weight 400g) bacteria-producing room is moved into time after inoculation, it is seen after inoculation Examine mycelium growth vigor, record mycelia purseful time, single bag of average product (the yield summation of first three tide is calculated as yield), calculation biology Rate (biological efficiency=every bag of fresh mushroom production/every bag of siccative weight × 100%), disease incidence is shown in Table 1;It is real to the son cultivated Body carries out the constituent contents such as phosphorus potassium and content of beary metal measurement, is shown in Table 2;To the Uricularia polytricha of picking carry out auricle color and its other The case where influence of character, is recorded, and is shown in Table 3.
Table 1 is to Uricularia polytricha upgrowth situation table
From the foregoing, it will be observed that comparing comparative example using culture medium prepared by the present invention, mycelium growth vigor is more vigorous, when mycelia purseful Between mention the last fortnight or more, single bag of average product is compared to increasing 118g or more, and biology rate is compared to improving at least 30% or more;Hair Sick number significantly reduces.
Influence of 2 culture material of table to element different in Uricularia polytricha fructification
Project Embodiment 1 Embodiment 2 Embodiment 3 Comparative example
P (%) 0.38 0.40 0.53 0.2
K (%) 1.12 1.28 1.29 0.8
Na(mg/kg) 1312 1321 1334 598
Ca(mg/kg) 1138 1142 1235 984
Mg(mg/kg) 2005 2021 2220 1140
Fe(mg/kg) 118 121 129 102
Zn(mg/kg) 15.0 15.0 16.1 10.1
As(mg/kg) 0.06 0.06 0.06 0.05
Hg(mg/kg) 0.03 0.02 0.02 0.03
Pb(mg/kg) < 0.5 < 0.5 < 0.5 < 0.5
Cd(mg/kg) < 0.1 < 0.1 < 0.1 < 0.1
As seen from the above table, the fructification picked using culture medium prepared by the present invention, phosphorus, potassium, sodium, calcium, magnesium, zinc and manganese contain It measures higher than the content of control group;According to " People's Republic of China's agricultural industry criteria " (green food edible mushroom NY/T749- 2012), fruit body of edible fungi dry product heavy metal standard: Pb < 2.0mg/kg, Cd < 1.0mg/kg, As < 1.0mg/kg, Hg < 0.2mg/kg, as shown in Table 2, four heavy metal species contents all meet green food standard in Uricularia polytricha fructification.
The case where each embodiment of table 3 is to auricle color and its other characters table
As shown in Table 3, using method of the invention, the color of fructification auricle is scarlet;On auricle side in the application Edge form, villus quantity, outside of belly rib ridge back are better than comparative example.
Test 2: the Uricularia polytricha cultivation matrix with diseases prevention prepared by embodiment 3 is experimental group, control CK1 group and implementation 3 difference of example is not added to the anti-microbial inoculum of trichoderma, other are same as Example 3;It compares CK2 group and 3 difference of embodiment is wood It is 3 × 10 that hook-shaped trichoderma is as prepared into its spore concentration by mould anti-microbial inoculum after inclined-plane culture8The seed of a spore/mL Liquid, then be inoculated with and cultivated as in solid-state fermentation culture medium, the solid-state fermentation culture medium is to add 15.0g sucrose waste liquid Enter 0.1g ammonium sulfate, 0.05g potassium dihydrogen phosphate, 0.04g dipotassium hydrogen phosphate and agar 15.0g, adjustment water content is 65%, is kept For pH naturally, in 131 DEG C of sterilizing 35min, other are same as Example 3;Embodiment 3, CK1, CK2 are also using test 1 Method cultivates Uricularia polytricha, and comparing embodiment 3, CK1, CK2 are shown in Table 4 to the pathological situation of Uricularia polytricha:
Table 4
Project Number of incidences Disease incidence reduces (%)
Embodiment 3 2 66.7
CK1 6 0
CK2 4 33.3
As shown in Table 4, the addition anti-microbial inoculum of trichoderma (CK2) is that the ingredient of Uricularia polytricha cultivation matrix can reduce time that disease occurs Number, and the ingredient that the anti-microbial inoculum of trichoderma (embodiment 3) is Uricularia polytricha cultivation matrix is made using Lightyellow Sophora Root, occur to during cultivation The slip of the number of disease is more preferably.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to In the covered the scope of the patents of the present invention.

Claims (7)

1. a kind of cultural method for improving Uricularia polytricha yield, it is characterised in that: comprising the following specific steps
(1) it builds canopy: surrounding long 8-10m × wide 5-6m × high 6-7m canopy body using several brackets, canopy body surrounding first covers screening Then cavity block covers black film, reuse surrounding of the locking bar in canopy body middle and upper part and successively join end to end and fix the black film of covering, And several support brackets, and support bracket and canopy body side length parallel arrangement are installed in canopy body medium position;The load-bearing branch Frame height is higher than bracket;Yang Peng and negative canopy are formed between support bracket and bracket, Yang Peng and negative canopy are arranged back-to-back, and mutually interconnect Logical, the roofing inclination of Yang Peng towards being arranged in the east, and the roofing inclination of negative canopy is arranged towards west, and the ridge of Yang Peng is higher than negative canopy Ridge, is equipped with vent passages between Yang Peng and negative canopy, vent passages are covered with black film, are all covered on Yang Peng and the roofing of negative canopy Black film, it is described sun canopy in be from top to bottom disposed with light-transmissive film layer, foam sheet and shading film layer, and light-transmissive film layer be close to sun The roofing of canopy is from top to bottom disposed with light-transmissive film layer, foam sheet and shading film layer in negative canopy, and light-transmissive film layer is close to yin The roofing of canopy;
(2) cultivation matrix inoculation and stacking: the inoculation for being inoculated with Uricularia polytricha strain is followed closely and is inoculated according to triangle disposition Through bacterium bag being stacked layer by layer then, fixed frame is reused by the bacterium bag after stacking and is fixed on canopy in the bacterium bag equipped with cultivation matrix In vivo and under film layer of shading;
The cultivation matrix specifically comprises the following steps:
A feedstock processing: the broken wood shavings of level-one, the broken wood shavings classification of second level are exposed to the sun 2-3 days under the sun;By Shell of Water Chestnut and sesame stalk Also 1-2cm partial size is crushed to after being exposed to the sun under the sun 3-4 days respectively;Value of Spartina Anglica and durian shell are dried to that holding is amphidromic Sense, is then crushed to 1-2cm partial size respectively;It is 1-2cm that garlic stalk, apple pomace are crushed to partial size respectively;By rape chopped straw After stalk high-temperature sterilization is dry after granular mill stirs, the solid particle that particle diameter is 0.3-0.7cm is obtained;By sauce Oily waste residue is handled to salt content by the desalinization of soil by flooding or leaching less than 1%;By Klinit dregs through peracid treatment, pH=5-6 is adjusted;
B fermentation: according to weight part ratio, by 5-15 parts of the sesame stalk after processing of step A, 5-15 parts of Shell of Water Chestnut, Value of Spartina Anglica 3-10 parts, 3-10 parts of durian shell, 3-10 parts of garlic stalk, 3-10 parts of apple pomace, 5-8 parts of Klinit dregs, the broken wood shavings 5-8 of level-one Part, 5-8 parts of rape flower stalk and 5-8 parts of soy sauce waste residue, with 3-5 parts of wheat bran, 5-10 parts of precipitated calcium carbonate and 5-15 parts of soybean powder It is mixed, adds milk of lime and water, adjust pH to 5-7, control moisture content 60-75%, obtain fermentation raw material, will ferment Raw material and the anti-microbial inoculum of 5-15 parts of trichodermas mix, and build heap fermentation 20-30h, 35-45 DEG C of fermentation temperature, fermentation material can be obtained;
C charging: moisture content is adjusted for 55-70 %, so using distilled water for broken wood shavings 5-8 parts of second level for treated in step A It is packed into culture bag afterwards, the broken wood shavings of adjustment second level form hollow form and are tightly attached on the outside of the internal layer of culture bag, then again by step B's Fermentation material is filled in hollow position, is wrapped in fermentation material in the broken wood shavings of second level, and then sealing obtains bag cultivating material;
D sterilizing: bag cultivating material is sterilized 30-45min at 121 DEG C, obtains the Uricularia polytricha cultivation matrix with diseases prevention;
(3) it training orientation: is covered with the shading film of canopy body surrounding in Uricularia polytricha culture medium bacterium bag after mycelia daily morning 10 O'clock to rolling during 12 points, remaining time puts down covering, until stopping after Uricularia polytricha fruiting;It will after Uricularia polytricha fruiting 3-8 days The black film covered on the roofing of Yang Peng is rolled;The foam sheet in positive canopy is dismantled after Uricularia polytricha fruiting 9-16 days;When hair wood Ear fruiting rolled up the black film that canopy body surrounding covers after 10-14 days during 3 points to 5 points of 10 points to 12 points of every morning and afternoon It rises, remaining time puts down covering, and so circulation is until Uricularia polytricha harvesting finishes.
2. a kind of cultural method for improving Uricularia polytricha yield according to claim 1, it is characterised in that: the trichoderma is fungi-proofing Agent is that Lightyellow Sophora Root is consolidated microbial inoculum with trichoderma to mix according to mass ratio 1:1, builds heap and ferments 15-20 days;The trichoderma consolidates bacterium Agent be hook-shaped trichoderma is prepared into after inclined-plane culture its spore concentration be 3 × 108The seed liquor of a spore/mL, then by its It is inoculated in solid-state fermentation culture medium and cultivates, the solid-state fermentation culture medium is that 0.1g sulfuric acid is added in 15.0g sucrose waste liquid Ammonium, 0.05g potassium dihydrogen phosphate, 0.04g dipotassium hydrogen phosphate and agar 15.0g, adjustment water content be 65%, keep pH naturally, in 131 DEG C of sterilizing 35min.
3. a kind of cultural method for improving Uricularia polytricha yield according to claim 1, it is characterised in that: the broken plane of level-one Colored length, thickness, wide size are 30-60 × 0.4-1.0 × 30-80mm;Length, thickness, the wide size of the broken wood shavings of second level be 3-8×0.1-0.15×0.15-0.25mm。
4. a kind of cultural method for improving Uricularia polytricha yield according to claim 1, it is characterised in that: the room of the Yang Peng The ridge of ridge and negative canopy is on the same vertical plane, and the difference in height between the ridge of the Yang Peng and the ridge of negative canopy is 0.3- 0.6m。
5. a kind of cultural method for improving Uricularia polytricha yield according to claim 1, it is characterised in that: in step (1), Pit digging is also carried out in canopy body, and ground level 0.2-0.3m external lower than canopy after pit digging.
6. a kind of cultural method for improving Uricularia polytricha yield according to claim 1, it is characterised in that: outside the canopy body It encloses and is additionally provided with dewatering ditch.
7. a kind of cultural method for improving Uricularia polytricha yield according to claim 1, it is characterised in that: described to stack layer by layer The number of plies be 8-12 layers.
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