CN1666589A - Bacterial strain isolation and cultivation technique for Chinese wild species big fat mushroom - Google Patents
Bacterial strain isolation and cultivation technique for Chinese wild species big fat mushroom Download PDFInfo
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Abstract
The invention related to technique of edible mushroom provides a cultivate technique and a method for separating bacterial strains Chinese wild mast mushroom. Wild source of mast mushroom in said invention is scarce for meeting the demand of market. Said invention performs artificial cultivation of mast mushroom by selection from different culture mediums and conditions. The technique of the invention is easy to master and its material for cultivating has broad source, as a result, can be extended generally in countryside.
Description
One, technical field:
The present invention relates to the edible mushroom technical field, is a kind of Chinese wild species big fat mushroom bacterial strain separation and Culture technology and cultivation method specifically.
Two, background technology:
The existing abroad cultivation of big fat mushroom, but it belongs to the high temperature modification bacterial strain.At present, do not see the cultivation report of low form bacterial strain as yet.In Hebei of China, there is wild low form bacterial strain on ground such as Qinghai, Xinjiang.Because of big fat mushroom has greatly, meat is thick, outward appearance U.S., and fine and tender taste is nutritious, and delicious flavour is loved by the people.Only depend on wild collection for a long time.But wild resource is few, is difficult to meet the need of market.
Three, summary of the invention:
The present invention wants to provide a kind of big fat mushroom (Agaricus bitorguis (Quel.) Sacc) Chinese wild strain separation and Culture technology and culture technique.Big fat mushroom bacterial classification of the present invention derives from Kangbao County, Hebei province wild mushroom, CGMCC preservation No.1255.
The big fat mushroom that the present invention relates to detects the both macro and micro morphological feature of identifying through Institute of Microorganism, Academia Sinica: sample fruit body hypertrophy, and white when fresh, dirty white to light eggshell look.The cap dipped beam is sliding or decorative pattern sample crackle arranged, edge-smoothing and to curls inward, diameter 8～10cm can reach 21cm when ripe, and the top is flat or recessed slightly.Bacterial context is thick, 1.5～2.5cm, and delicacy, solid, white, the injury variable color is not obvious, tool mushroom tone smell.Stem is sturdy, and is cylindrical, white, long 2～6cm, thick 4～5cm, the thickest 9cm that reaches.Lamella is from life, and is closeer, initial stage digested tankage look, and back browning look is to brown-black, and is not isometric.The collarium bilayer, thick, white is given birth among the handle.The lamella small pieces are put into 2% KOH liquid and are observed spore, and oval contains oil droplet to subsphaeroidal, smooth, 6～8 * 5.5～6.2 μ m, and variable color is not obvious in melzen liquid.The wide bar-shaped or wide ellipse of pleat edge utricule is grown thickly, colourless or be with light yellow, 24～33 * 6.1～10 μ m.
Features such as sample form show, belong to basidiomycetes, Agaricales Agaricales, and Agaricus edibilis Agaricaceae, Agaricus Agaricus, big fat mushroom Agaricus bitorguis (Quel.) Sace finds that the ground people claim fragrant fertile mushroom again, the meat dried mushroom.Its bacterial context plumpness, delicious.Natural distribution in Hebei, Qinghai, Xinjiang etc.
Bacterial strain separation and Culture technology of the present invention is by the following method:
One, female separation and Culture of planting:
1, media components prescription:
Potato 200g, glucose 20g, agar powder 18g, water 1000ml,
PH7-7.5; Or potato 185g, wheat bran 15g, glucose 20g, agar powder 18g, water 1000ml.Or in above-mentioned two prescriptions, add potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, Cobastab more respectively
110mg.
2, medium is made:
With peeling potatoes, shave, add water boil 7-10 minute earlier, cross leaching juice.Add glucose, agar powder and batching potassium dihydrogen phosphate, magnesium sulfate then, boil molten mixing all, and supply the water consumption in the original formulation.
The solution of boiling is sub-packed in vitro while hot, and every 10ml is with cotton balls stoppered test tube mouth.By per five arms is a bundle, wraps an end that is plugged with cotton balls with brown paper, puts into high-pressure sterilizing pot, heats 121-126 ℃.Pressure 1.1-1.5kg/cm
2, sterilized 30 minutes.Take out test tube pendulum inclined-plane, solidify the back and be mother culture media.
3, separation and Culture:
Gather new fresh sporophore, carry out the epidermis sterilization.Under aseptic condition, get a fritter meat bacteria organization and move on the medium slant, place 18-20 ℃ to cultivate 15-20 days down.Mycelia is covered with 2/3 of medium slant, then is female the kind.
Two, original seed or cultivated species production:
1, composts or fertilisers of cultivating prescription:
Wheat berry or grain or cotton seed hull 97%, white sugar, calcium carbonate, land plaster each 1%, add potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, PH7-7.5 by above-mentioned batching total amount again.
2, spice:
With wheat or grain water maceration, admix white sugar, land plaster, calcium carbonate, potassium dihydrogen phosphate, magnesium sulfate equably after the reusable heat poach is softening thoroughly earlier; When making major ingredient with cotton seed hull, water is fully dark and damp, and heap fermentation 1-2 days, water content 60% is admixed white sugar, land plaster, calcium carbonate, potassium dihydrogen phosphate, magnesium sulfate again;
3, bottling or pack:
Produce original seed: selection standard seed bottle or glucose infusion liquid bottle or the Cans composts or fertilisers of cultivating of packing into, to eighty per cant full, seal with old newspaper and polypropylene screen; Produce to carry and to cultivate: because of consumption greatly except that with seed bottle, glucose infusion liquid bottle or the old Cans, the 1-2 strata propylene bag of the opening of available long 18-20cm, wide 12-15cm, every packed composts or fertilisers of cultivating height 10-12cm seals or seals with supporting seal mouth ring with cotton rope tying or running-on neck ring plug cotton balls method.
4, sterilization:
Adopt autoclaving, heat 121-126 ℃, pressure 1.1-1.5kg/cm
2, kept 1.5-2 hour with the need of seed bottle charging; Need keep 2 hours with the Polypropylene Bag charging.Adopt the sterilization of normal pressure equipment, 100 ℃ of temperature were kept 8-10 hour.
5, cooling:
Sterilization back seed bottle or strain bag, put under the transfer room natural temperature, be cooled to 20-23 ℃, prepare inoculation.
6, inoculation:
Produce original seed: under aseptic condition, get female charge level central authorities in the access bottle of planting; Produce cultivated species: get original seed 2-3g and insert in bottle or the bag on the charge level under aseptic condition, it is even to scatter.Method during with composts or fertilisers of cultivating bottling or pack is sealed.
7, cultivate:
Bottle that inserts bacterial classification or bag, to put on the culturing rack of culturing room through sterilizing, placement is neat, at temperature 18-20 ℃, under the condition of relative moisture 70%, cultivates 30-35 days, and mycelia is covered with bottle or bag.It is pure white, dense to reach mycelia, and caking capacity is strong, the standard of no living contaminants.
Bacterial strain cultivation method of the present invention:
Cultivation method of the present invention is applicable to a formula cultivation, trench cultivation, the cultivation of furrow formula.
1, cultivate material configuration proportion:
Argol with wheat class or the dried straw grass of crops such as paddy rice or corn or beans or tame hay and domestic animal, poultry is just made major ingredient, allocates phosphate fertilizer into, gypsum is made auxiliary material.Configuration proportion calculates dried straw grass of crop or tame hay 16-20kg with major ingredient 40kg with every square metre.Argol is 24-20kg just, auxiliary material phosphate fertilizer, each 400g of land plaster.
2, cultivate material fermentation process:
Water is fully cloudy saturating respectively with major ingredient earlier, builds the heap fermentation.Nature temperature bottom fermentation when piling interior temperature 60-70 ℃, carried out the turning first time about 7 days, and added auxiliary material.The interior temperature of heap is kept more than 80 ℃ and was carried out the turning second time in one day after 5-7 days.Turned over once, and turned over 3-5 time continuously in later every 6-7 days.The fermentation of cultivate material deepens brown to reach the straw grass, and contains a large amount of actinomyces albuses, has poor flavor, and water content 55-60% is a standard.
3, cultivate material is gone to bed and is inoculated vaccine:
Expect to go to bed thickness 20-24cm, take the method for broadcasting sowing, every square metre is inserted 1.5 standard jar bacterial classifications, and at bacterial classification loam cake cultivate material 2cm.
4, mycelial growth period management:
Behind the sowing bacterial classification, cultivating chamber should be closed ventilating opening tight and preserve moisture, and after the mycelial growth degree of depth reached 5-10cm, ventilated once every day, each 30-40 minute; After the mycelial growth degree of depth reaches 15-18cm, ventilate every day 2 times, each 1-2 hour, the ground water-sprinkling kept relative air humidity about 75%, and temperature maintenance is at 18-21 ℃.
5, earthing and management:
After the mycelial growth degree of depth reaches 20cm, should in time cultivate earthing on the charge level.Require with fertile loam, water content 18-20%, thickness of earth covering 2-3cm..Ventilate every day round the clock, and spray thin water once on earthing, and the earthing water content is not less than 18%, and indoor maintenance 16-18 ℃, relative air humidity is more than 80%.
6, management of producing mushroom:
Ventilate every day round the clock, the ground water-sprinkling once, spray thin water once every day on the earthing, relative air humidity 85-90%, indoor temperature 10-18 ℃.Prevent above 18 ℃.
7, gather:
When fruit body was medium well, the edge of cap curls inward still was adjacent on stem, began to gather.
That big fat mushroom bacterial strain of the present invention has is big, meat is thick, outward appearance is beautiful, fine and tender taste is nutritious, the characteristics of delicious flavour.The amino acid content of it and straw mushroom, mushroom, Hericium erinaceus is compared as follows:
Straw mushroom mushroom Hericium erinaceus Agaricus bitorqui bacterial strain
Amino acid kind 16 16 16 18
Total amino acid content 19.64 13.67 19.69 27.49
(mg/100g)
Human body must amino 7778
Acid kind (kind)
Must total amino acid content 7.41 4.88 7.26 9.42
(mg/100g)
Big fat mushroom bacterial strain separation and Culture of the present invention and cultivation method are through selecting different medium, different cultural methods.Wild big fat mushroom bacterial strain domestication, artificial cultivation have been realized.And the cultivate material source is wide, and the cultivation cost is low, and the unit are gross yield reaches 8.8-9.5Kg/m
2
China's wild species big fat mushroom bacterial strain compares with external Agaricus bitorqui strain growth temperature:
The external Agaricus bitorqui bacterial strain of China's wild species big fat mushroom bacterial strain
Mycelial growth temperature
5～25 ℃ 7～36 ℃ of scopes
18～20 ℃ 24～31 ℃ of suitable growth temperatures
Sporophore growth is sent out
Educate 5～20 ℃ 20～29 ℃ of temperature
Optimum growth temperature
1～18 ℃ 25～27 ℃ of scopes
Still continue to be difficult to below 26 ℃ differentiation under 5 ℃ of the particular points
Fruiting and growth fruit body
Four, embodiment:
Separate the cultivation technology:
Embodiment 1: take by weighing peeling potato 200g, shave.Add water 1000ml, boiled 7-10 minute, cross leaching juice.Add glucose 20g, agar powder 18g and batching potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g then, Cobastab
110mg boils molten mixing all, and supplies the water consumption in the original formulation.Control PH7-7.5
The solution of boiling is sub-packed in vitro while hot, and every 10ml is with cotton balls stoppered test tube mouth.By per five arms is a bundle, wraps an end that is plugged with cotton balls with brown paper, puts into high-pressure sterilizing pot, heats 121-126 ℃.Manometer 1.1-1.5kg/cm
2, sterilized 30 minutes.Take out test tube pendulum inclined-plane, solidify the back and be mother culture media.
Gather new fresh sporophore, carry out the epidermis sterilization.Under aseptic condition, get a fritter meat bacteria organization and move on the medium slant, place 18-20 ℃ to cultivate 10-15 days down.Mycelia is covered with 2/3 of medium slant, then is female the kind.
Embodiment 2: take by weighing peeling potato 200g, shave.Add water 1000ml, boiled 7-10 minute, cross leaching juice.Add glucose 20g, agar powder 18g then and boil molten mixing all, and supply the water consumption in the original formulation.Control PH7-7.5.Following method is with embodiment 1.
Embodiment 3:
Remove skin potato 185g, shave, claim wheat bran 15g, add water 1000ml, boiled 7-10 minute, cross leaching juice, add glucose 20g, agar powder 18g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, Cobastab then
110mg boils molten mixing thoroughly, supplies the water consumption in the original formulation, and following method is with embodiment 1.
The original seed culture technique:
Embodiment 4: take by weighing wheat berry 9.7Kg,, white sugar, calcium carbonate, each 0.1Kg of land plaster, potassium dihydrogen phosphate 10g, magnesium sulfate 5g.With wheat water maceration, admix white sugar, land plaster, calcium carbonate, potassium dihydrogen phosphate, magnesium sulfate equably after the reusable heat poach is softening thoroughly earlier; Control PH7-7.5.
The selection standard seed bottle composts or fertilisers of cultivating of packing into to eighty per cant full, seals with old newspaper and polypropylene screen.Adopt autoclaving, heat 121-126 ℃, pressure 1.1-1.5kg/cm
2, kept 1.5-2 hour; The seed bottle after the sterilization, put under the transfer room natural temperature, be cooled to 20-23 ℃, prepare inoculation.Under aseptic condition, get female charge level central authorities in the access bottle of planting; Method when bottling with composts or fertilisers of cultivating is sealed.The bottle that inserts bacterial classification, put on the culturing rack of culturing room through sterilizing, place neatly, at temperature 18-20 ℃, under the condition of relative air humidity 70%, to cultivate 30-35 days, mycelia is covered with bottle.It is pure white, dense to reach mycelia, and caking capacity is strong, the standard of no living contaminants.
Embodiment 5: take by weighing cotton seed skin 9.7Kg, white sugar, calcium carbonate, each 0.1Kg of land plaster, potassium dihydrogen phosphate 10g, magnesium sulfate 5g.Earlier that cotton seed skin water is fully dark and damp, and heap fermentation 1-2 days, water content is admixed white sugar, land plaster, calcium carbonate, potassium dihydrogen phosphate, magnesium sulfate at 60-65%; Control PH7-7.5.Select the old Cans composts or fertilisers of cultivating of packing into for use,, seal with old newspaper and polypropylene screen to eighty per cant full.Following method is with embodiment 4.
Cultivation method:
Embodiment 6: calculate with major ingredient 40Kg with every square metre, take by weighing Wheat Straw grass 20Kg, dried poultrymanure 20Kg, auxiliary material phosphate fertilizer, gypsum, each 400g.Water is abundant cloudy saturating respectively with major ingredient earlier, is piled into the long vertical cuboid fermentation heap of not limitting all around of tolerance 1.5m, high 1.5m by one deck straw one deck chicken manure.Nature temperature bottom fermentation when piling interior temperature 60-70 ℃, carried out the turning first time about 7 days, and added auxiliary material.The interior temperature of heap is kept more than 80 ℃ and was carried out the turning second time in one day after 5-7 days.Turned over once, and turned over 3-5 time continuously in later every 6-7 days.The fermentation of cultivate material reaches the straw grass and deepens brown, and contains a large amount of actinomyces albuses, has poor flavor, and water content 55-60% is a standard.
Select the cultivation of bed formula, expect to go to bed thickness 20-24cm, take the method for broadcasting sowing, every square metre is inserted 1.5 standard jar bacterial classifications, and at bacterial classification loam cake cultivate material 2cm.Behind the sowing bacterial classification, cultivating chamber should be closed ventilating opening tight and preserve moisture, and after the mycelial growth degree of depth reached 5-10cm, ventilated once every day, each 30-40 minute; After the mycelial growth degree of depth reaches 15-18cm, ventilate every day 2 times, each 1-2 hour, the ground water-sprinkling kept relative air humidity about 75%, and temperature maintenance is at 18-20 ℃.After the mycelial growth degree of depth reaches 20cm, should in time cultivate earthing on the charge level.Require with fertile loam, water content 18-20%, thickness of earth covering 2-3cm..Ventilate every day round the clock, and spray thin water once on earthing, and the earthing water content is not less than 18%, and indoor maintenance 16-18 ℃, relative air humidity is more than 80%.After occurring original hase or little young mushroom in the earthing.Ventilate every day round the clock, the ground water-sprinkling once, spray thin water once every day on the earthing, relative air humidity 85-90%, indoor temperature 10-18 ℃.Prevent above 18 ℃.When fruit body was medium well, the edge of cap curls inward still was adjacent on stem, began to gather.
Embodiment 7: take by weighing corn stalk 16Kg, do cow dung 24Kg auxiliary material phosphate fertilizer, each 400g of land plaster.Fermentation process is with embodiment 6.Wide 1.5m, the long trench of not limitting of dark 0.5m are dug in selectively ditch cultivation, ground.With the mound high 0.3m around ditch that digs out, very useful wooden rafter of ditch or thick bamboo bar are barricaded as frame, cover polyethylene film on the frame again, add a cover straw grass or straw screen or mat on the film and shelter from heat or light, preserve moisture.Cultivation and management method are with embodiment 6.
Embodiment 8: take by weighing tame hay 18Kg, dried sheep excrement 22Kg auxiliary material phosphate fertilizer, each 400g of land plaster.Fermentation process is with embodiment 6.Select the cultivation of furrow formula, the ground bedding, wide 1.5m, dark 0.2m, length is not limit, all around bedding ridge height 0.2m.The furrow ridge waddy of putting on the shelf, lid polypropylene screen and straw screen or mat shelter from heat or light, preserve moisture.Cultivation and management method are with embodiment 6.
Embodiment 9: take by weighing beans straw 18Kg, dried pig manure 22Kg auxiliary material phosphate fertilizer, each 400g of land plaster.Fermentation process is with embodiment 6.Select the cultivation of bed formula, cultivation and management method are with embodiment 6.
Claims (6)
1, a kind of Chinese wild species big fat mushroom bacterial strain separation and Culture technology is characterized by, by the following method step:
One, female separation and Culture of planting:
(! ), the media components prescription:
Potato 200g, glucose 20g, agar powder 18g, water 1000ml, PH7-7.5; Or potato 185g, wheat bran 15g, glucose 20g, agar powder 18g, water 1000ml.Or in above-mentioned two prescriptions, add potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, Cobastab more respectively
110mg;
(2), medium is made:
First with peeling potatoes, shave, or plus wheat bran adds water boil 7-10 minute, mistake leaching juice; Add glucose, agar powder then or add potassium dihydrogen phosphate, magnesium sulfate, Cobastab
1, boil molten mixing all, and supply the water consumption in the original formulation;
The solution of boiling is sub-packed in vitro while hot, and every 10ml with cotton balls stoppered test tube mouth, is an a bundle by per five arms, wraps an end that is plugged with cotton balls with brown paper, puts into high-pressure sterilizing pot, heats 121-126 ℃, pressure 1.1-1.5kg/cm
2, sterilized 30 minutes, take out test tube pendulum inclined-plane, solidify the back and be mother culture media;
(3), separation and Culture:
Gather new fresh sporophore, carry out the epidermis sterilization; Get a fritter meat bacteria organization and move on the medium slant under aseptic condition, place 18-20 ℃ to cultivate 15-20 days down, mycelia is covered with 2/3 of medium slant, then is female the kind;
Two, original seed or cultivated species production:
(1), composts or fertilisers of cultivating prescription:
Wheat berry or grain or cotton seed hull 97%, white sugar, calcium carbonate, land plaster each 1%, add potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, PH7-7.5 by above-mentioned batching total amount again;
(2), spice:
With wheat or grain water maceration, admix white sugar, land plaster, calcium carbonate, potassium dihydrogen phosphate, magnesium sulfate equably after the reusable heat poach is softening thoroughly earlier; When making major ingredient with cotton seed hull, water is fully dark and damp, and heap fermentation 1-2 days, water content 60% is admixed white sugar, land plaster, calcium carbonate, potassium dihydrogen phosphate, magnesium sulfate again;
(3), bottling or pack:
Produce original seed: selection standard seed bottle or glucose infusion liquid bottle or the Cans composts or fertilisers of cultivating of packing into, to eighty per cant full, seal with old newspaper and polypropylene screen; Produce cultivated species: because of consumption greatly except that with seed bottle, glucose infusion liquid bottle or the old Cans, the 1-2 strata propylene bag of the opening of available long 18-20cm, wide 12-15cm, every packed composts or fertilisers of cultivating height 10-12cm seals or seals with supporting seal mouth ring with cotton rope tying or running-on neck ring plug cotton balls method;
(4), sterilization:
Adopt autoclaving, heat 121-126 ℃, pressure 1.1-1.5kg/cm
2, kept 1.5-2 hour with the need of seed bottle charging; Need keep 2 hours with the Polypropylene Bag charging; Adopt the sterilization of normal pressure equipment, 100 ℃ of temperature were kept 8-10 hour;
(5), cooling:
Sterilization back seed bottle or strain bag, put under the transfer room natural temperature, be cooled to 20-23 ℃, prepare inoculation;
(6), inoculation:
Produce original seed: under aseptic condition, get female charge level central authorities in the access bottle of planting; Produce cultivated species: get under aseptic condition that original seed 2-3g connects that bottle is gone into or bag on the charge level, scatter evenly, with the composts or fertilisers of cultivating bottling or the method when packing seal;
(7) cultivate:
Bottle that inserts bacterial classification or bag, to put on the culturing rack of culturing room through sterilizing, placement is neat, at temperature 18-20 ℃, under the condition of relative moisture 70%, cultivated 30-35 days, mycelia is covered with bottle or bag, it is pure white, dense to reach mycelia, and caking capacity is strong, the standard of no living contaminants.
2, a kind of Chinese wild species big fat mushroom bacterial strain separation and Culture technology as claimed in claim 1, it is characterized by: described mother culture media is potato 200g, glucose 20g, agar powder 18g, water 1000ml.
3, a kind of Chinese wild species big fat mushroom bacterial strain separation and Culture technology as claimed in claim 1, it is characterized by: described mother culture media is potato 185g, wheat bran 15g, glucose 20g, agar powder 18g, water 1000ml.
4, a kind of Chinese wild species big fat mushroom bacterial strain separation and Culture technology as claimed in claim 1, it is characterized by: described mother culture media is potato 1858, wheat bran 15g, glucose 20g, agar powder 18g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, Cobastab
110mg, water 1000ml.
5, a kind of Chinese wild species big fat mushroom bacterial strain separation and Culture technology as claimed in claim 1, it is characterized by: described mother culture media is potato 200g, glucose 20g, agar powder 18g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, Cobastab
110mg, water 1000ml.
6, a kind of cultivation method of Chinese wild species big fat mushroom bacterial strain is characterized by:
(1), cultivate material configuration proportion:
Argol with wheat class or paddy rice or the dried straw grass of other crop such as corn or beans or tame hay and domestic animal, poultry is just made major ingredient, allocate phosphate fertilizer into, gypsum is made auxiliary material, configuration proportion, calculate with major ingredient 40kg with every square metre, dried straw grass of crop or tame hay 16-20kg, argol is 24-20kg just, auxiliary material phosphate fertilizer, each 400g of land plaster;
(2), cultivate material fermentation process:
Water is fully cloudy saturating respectively with major ingredient earlier, stacks fermentation, and natural temperature bottom fermentation is about 7 days, when piling interior temperature 60-70 ℃, carry out the turning first time, and add auxiliary material, the interior temperature of heap is kept more than 80 ℃ and was carried out the turning second time in one day after 5-7 days, turned over once in later every 6-7 days, turn over 4-5 time continuously, the fermentation of cultivate material reaches the straw grass, and to deepen brown be standard, and contain a large amount of actinomyces albuses, has poor flavor, water content 55-60%;
(3), cultivate material is gone to bed and is inoculated vaccine:
Expect to go to bed thickness 20-24cm, take the method for broadcasting sowing, every square metre is inserted 1.5 standard jar bacterial classifications, and at bacterial classification loam cake cultivate material 2cm;
(4), mycelial growth period management:
Behind the sowing bacterial classification, cultivating chamber should be closed ventilating opening tight and preserve moisture, and after the mycelial growth degree of depth reached 5-10cm, ventilated once every day, each 30-40 minute; After the mycelial growth degree of depth reaches 15-18cm, ventilate every day 2 times, each 1-2 hour, the ground water-sprinkling kept relative air humidity about 75%, and temperature maintenance is at 18-21 ℃;
(5), earthing and management:
After the mycelial growth degree of depth reaches 20cm, should in time cultivate earthing on the charge level; Require with fertile loam, water content 18-20%, thickness of earth covering 2-3cm, ventilate every day round the clock, and spray thin water once on earthing, and the earthing water content is not less than 18%, and indoor maintenance 16-18 ℃, relative air humidity is more than 80%;
(6), management of producing mushroom:
Ventilate every day round the clock, the ground water-sprinkling once, spray thin water once every day on the earthing, relative air humidity 85-90%, prevents above 18 ℃ by indoor temperature 10-18 ℃;
(7), gather:
When fruit body was medium well, the edge of cap curls inward still was adjacent on stem, began to gather.
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| CN1189288A (en) * | 1998-03-02 | 1998-08-05 | 宫银慧 | Method for culture of double-spore mushroom and Jitui mushroom |
| CN1084138C (en) * | 1998-04-07 | 2002-05-08 | 杭州常青保健食品有限公司 | High temperature resistant mushroom and its breeding and cultivating method |
| CN1457631A (en) * | 2003-03-07 | 2003-11-26 | 关弦敏 | Cultivating technology for brown mushroom |
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