CN102812854A - Manual wild-imitation cultivation technology for pure strains of grifola - Google Patents
Manual wild-imitation cultivation technology for pure strains of grifola Download PDFInfo
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Abstract
The invention relates to a manual wild-imitation cultivation technology for pure strains of grifola. The manual wild-imitation cultivation technology comprises the steps of land selection and land preparation, cultivation, seeding methods, management after seeding, management in a coming year, management in a third year, excavation and drying processing. In a biological angle, wild sclerotium tubers are polluted in the natural environment, and purity of the pure strains is achieved. Besides, the pure strain only have fungus age of half a year, wild grifola fungus tubers have the longest fungus age of five years, and the reproduction rate of the pure strains is one time more than that of the wild grifola fungus tubers.
Description
Technical field
The present invention relates to a kind of culture technique, be specifically related to the artificial imitative wild culture technique of a kind of umbellate pore furgus pure culture.
Background technology
Umbellate pore furgus, another name wild boar Siberian cocklebur, polyporus umbellatus, chicken droppings Siberian cocklebur.Belong to Basidiomycotina, Hymenomycetes, Holobasidiomycetidae, Aphyllophorales, Polyporaceae, Polyporus.Umbellate pore furgus contains grifola polysaccharide and ergosterol etc.Be used as medicine with sclerotium.Effects such as favourable water, excreting dampness find that in recent years it has certain curative effect to hepatitis B.Umbellate pore furgus is all by excavating wild hyoscine since ancient times, because natural resources is few, the survival dose increase is so disparities between supply and demand are outstanding day by day.
Increase along with the Chinese medicine market demand; The minimizing of wild resource, the gaps in market is increasing, and price is constantly soaring; Though the popularization that has wild Siberian cocklebur artificial graft and armillaria mellea to mix in recent years to plant report; But because excessive cycle or output shakiness can not be met the need of market all the time, the author selected the umbellate pore furgus pure culture so far first from 1993, culture technique is constantly studied updated; The main cause of finding restriction umbellate pore furgus output and excessive cycle is because due to the cell age of armillaria mellea, so taked the existing culture technique of pure armillaria mellea of artificial culture and the open-air natural propagation of pure umbellate pore furgus bacterial classification.
Summary of the invention
The object of the present invention is to provide the artificial imitative wild culture technique of umbellate pore furgus pure culture that a kind of output is high, growth cycle is short.
The present invention's technical scheme of being taked of dealing with problems is: the artificial imitative wild culture technique of a kind of umbellate pore furgus pure culture, and it may further comprise the steps:
(1), choosing ground and whole ground: generally take Schattenseite according to different regions, the Northeast selects the steeper hillside fields of tailo to be advisable, gradient 15-45 degree, promptly except that covering summer the shade, there is drainage condition preferably in the forest land of ground temperature in summer (0.2M is dark) below 25 ℃;
(2), cultivation: make the three-class strain of (umbellate pore furgus is special-purpose) armillaria mellea cultivation bag and umbellate pore furgus prior to seeding about 3 months, to guarantee the high and stable yields of umbellate pore furgus, sowing should be adopted spring sowing, goes back up to 14 ℃ with local ground temperature (0.2M is dark) and is advisable;
(3), the making of armillaria mellea cultivation bag:
A, prescription and medium preparation:
B, pack are sterilized, and select the 15*30*0.05cm bag, and sack filling machine (or hand) pack must be adorned reality, and sack is tightened with a mouthful rope folding mouth, and the outer bag of overcoat 17*33*0.04cm carries out normal-pressure sterilization, and (in 500 bags) clocked and kept 24 hours when temperature reached 100 ℃;
C, inoculated and cultured: adopt inoculating hood, semi-solid original seed, the cave inoculation of punching; The good outer bag of cover gets into unglazed training chamber, in the cultivation temperature 15D; Room temperature is controlled at 19～21 ℃, is controlled at 16～19 ℃ to the purseful cultivation temperature behind the 15D and is advisable, and it is on probation that the mycelia purseful promptly can be used for cultivation;
(4), Polyporus strains Production: specific practices: 90% of corn grain, high-rot fine sawdust 10%, through the heart of the corn boil, remove and fine sawdust mix, then add the weight and fine sawdust, etc. water, and then continue Hun mix, marinated that? 750ml bottle or 500ml standard strains of sugar water bottle, sealed immediately after sterilization, sterilization up to 100 ℃ 24h atmospheric pressure above, autoclave reached 0.14 ~ 0.15mPa (depending on container ) 2.5 ~ 3.5h, cooled, and the access Polyporus two kinds, in the light culture room, to 18 ~ 22 ℃ temperature of cultivation to the finished product;
(5), type of seeding:
A, be as the criterion, need 25 bags of armillaria mellea, 2 bottles in umbellate pore furgus three-class strain 750ml bottle (3 bottles in 500ml syrup bottle), fresh thin brush wood filler (diameter 0.02～0.05m, long 0.2m) 20kg, detritus soil (or sandy soil) 0.2m3 with every cave (1 ㎡);
B, go back up to 14 ℃ according to local microclimate ground temperature after, choosing is fine, along sloping gesture excavation 1m, the sowing hole of dark 0.2m; 25 bags of armillaria mellea are sloughed packing and evenly are lying in the hole, are the 0.8m*0.8m area, learn and stay the 0.1m space week, fill up peripheral 0.1m space and bury armillaria mellea bag 1/2 with detritus soil; Umbellate pore furgus bacterial classification (as far as possible being block) is put on the armillaria mellea limit, every bag of both sides are put one to two, fill out with detritus soil not have the umbellate pore furgus bacterial classification again again; Again the fresh inserts of 20kg is evenly put on it, filled out detritus soil while putting, until burying filler; Upward dig above the cave epidermis overlies with former, be turtleback in order to draining, succeeding planting will stay the drain ditch in the ranks in flakes;
(6), management after planting: thermophilic is after planting made regular check on temperature in the cave, must on the cave, add a cover the thing that shades as surpassing 26 ℃, as is lower than 18 ℃ and should adds intense light irradiation; The draining in time when rainy in summer needs on the cave, suitably water low amounts of water (being advisable with enough moisture 0.1-0.15) especially during arid, in addition generally all being main naturally;
(7), management in the coming year: on source points, add a cover the detritus soil of 0.1m, have the crack on the cave or have grey Siberian cocklebur piece to occur as finding behind the rain summer, should continue to add the 0.1m detritus soil again, and other are identical with first year;
(8), management in the 3rd year: along with the conversion of filler nutrition, the increase of Siberian cocklebur piece breeding rate is behind the beginning of spring temperature recovery; Inspection Siberian cocklebur piece is apart from surface distance; It is thick generally once to cover source points ground surface soil 0.15m, and the broad-leaved fallen leaves of 0.1-0.15m are covered in summer has set in again, until gather after autumn;
(9), when ground temperature is reduced to below 14 ℃ after autumn, can excavate, can the superiors' epidermis be removed when excavating, can see the Siberian cocklebur piece; Gradually Siberian cocklebur piece and filler are taken out from one side, the most advanced and sophisticated linen Siberian cocklebur piece of will growing, 1-1.5kg is reserved in every cave; Filling branch material with not rotting fully is embedded in the source points, and other Siberian cocklebur pieces are as the oven dry of commercially available medicine Siberian cocklebur, and most advanced and sophisticated young Siberian cocklebur is cooked kind in the source points plantation; Plant and excavated in back 2 years, the old cave after the plantation can not be planted continuously again, needs to plant after 2 years at interval again;
(10), drying processing; The bright Siberian cocklebur that digs is back cleaned one day warm air drier of packing into of airing with clear water, and temperature is in oven dry below 60 ℃, and the baking back packs with bag film.
There has been pollution in the present invention from the wild sclerotium stem tuber of biological angle umbellate pore furgus natural environment, the determined problem of purity of purebred fungi degradation.Moreover purebred bacterium has only the cell age of half a year, and wild umbellate pore furgus bacterium stem tuber the longest reach 5 years, reproduction rate of the present invention is Duoed one times than it.
Embodiment:
Plant the artificial imitative wild culture technique of umbellate pore furgus pure culture, it may further comprise the steps:
(1), choosing ground and whole ground: generally take Schattenseite according to different regions, the Northeast selects the steeper hillside fields of tailo to be advisable, gradient 15-45 degree, promptly except that covering summer the shade, there is drainage condition preferably in the forest land of ground temperature in summer (0.2M is dark) below 25 ℃;
(2), cultivation: make the three-class strain of (umbellate pore furgus is special-purpose) armillaria mellea cultivation bag and umbellate pore furgus prior to seeding about 3 months, to guarantee the high and stable yields of umbellate pore furgus, sowing should be adopted spring sowing, goes back up to 14 ℃ with local ground temperature (0.2M is dark) and is advisable;
(3), the making of armillaria mellea cultivation bag:
A, prescription and medium preparation:
B, pack are sterilized, and select the 15*30*0.05cm bag, and sack filling machine (or hand) pack must be adorned reality, and sack is tightened with a mouthful rope folding mouth, and the outer bag of overcoat 17*33*0.04cm carries out normal-pressure sterilization, and (in 500 bags) clocked and kept 24 hours when temperature reached 100 ℃;
C, inoculated and cultured: adopt inoculating hood, semi-solid original seed, the cave inoculation of punching; The good outer bag of cover gets into unglazed training chamber, in the cultivation temperature 15D; Room temperature is controlled at 19～21 ℃, is controlled at 16～19 ℃ to the purseful cultivation temperature behind the 15D and is advisable, and it is on probation that the mycelia purseful promptly can be used for cultivation;
(4), Polyporus strains Production: specific practices: 90% of corn grain, high-rot fine sawdust 10%, through the heart of the corn boil, remove and fine sawdust mix, then add the weight and fine sawdust, etc. water, and then continue Hun mix, marinated that? 750ml bottle or 500ml standard strains of sugar water bottle, sealed immediately after sterilization, sterilization up to 100 ℃ 24h atmospheric pressure above, autoclave reached 0.14 ~ 0.15mPa (depending on container ) 2.5 ~ 3.5h, cooled, and the access Polyporus two kinds, in the light culture room, to 18 ~ 22 ℃ temperature of cultivation to the finished product;
(5), type of seeding:
A, be as the criterion, need 25 bags of armillaria mellea, 2 bottles in umbellate pore furgus three-class strain 750ml bottle (3 bottles in 500ml syrup bottle), fresh thin brush wood filler (diameter 0.02～0.05m, long 0.2m) 20kg, detritus soil (or sandy soil) 0.2m3 with every cave (1 ㎡);
B, go back up to 14 ℃ according to local microclimate ground temperature after, choosing is fine, along sloping gesture excavation 1m, the sowing hole of dark 0.2m; 25 bags of armillaria mellea are sloughed packing and evenly are lying in the hole, are the 0.8m*0.8m area, learn and stay the 0.1m space week, fill up peripheral 0.1m space and bury armillaria mellea bag 1/2 with detritus soil; Umbellate pore furgus bacterial classification (as far as possible being block) is put on the armillaria mellea limit, every bag of both sides are put one to two, fill out with detritus soil not have the umbellate pore furgus bacterial classification again again; Again the fresh inserts of 20kg is evenly put on it, filled out detritus soil while putting, until burying filler; Upward dig above the cave epidermis overlies with former, be turtleback in order to draining, succeeding planting will stay the drain ditch in the ranks in flakes;
(6), management after planting: thermophilic is after planting made regular check on temperature in the cave, must on the cave, add a cover the thing that shades as surpassing 26 ℃, as is lower than 18 ℃ and should adds intense light irradiation; The draining in time when rainy in summer needs on the cave, suitably water low amounts of water (being advisable with enough moisture 0.1-0.15) especially during arid, in addition generally all being main naturally;
(7), management in the coming year: on source points, add a cover the detritus soil of 0.1m, have the crack on the cave or have grey Siberian cocklebur piece to occur as finding behind the rain summer, should continue to add the 0.1m detritus soil again, and other are identical with first year;
(8), management in the 3rd year: along with the conversion of filler nutrition, the increase of Siberian cocklebur piece breeding rate is behind the beginning of spring temperature recovery; Inspection Siberian cocklebur piece is apart from surface distance; It is thick generally once to cover source points ground surface soil 0.15m, and the broad-leaved fallen leaves of 0.1-0.15m are covered in summer has set in again, until gather after autumn;
(9), when ground temperature is reduced to below 14 ℃ after autumn, can excavate, can the superiors' epidermis be removed when excavating, can see the Siberian cocklebur piece; Gradually Siberian cocklebur piece and filler are taken out from one side, the most advanced and sophisticated linen Siberian cocklebur piece of will growing, 1-1.5kg is reserved in every cave; Filling branch material with not rotting fully is embedded in the source points, and other Siberian cocklebur pieces are as the oven dry of commercially available medicine Siberian cocklebur, and most advanced and sophisticated young Siberian cocklebur is cooked kind in the source points plantation; Plant and excavated in back 2 years, the old cave after the plantation can not be planted continuously again, needs to plant after 2 years at interval again;
(10), drying processing; The bright Siberian cocklebur that digs is back cleaned one day warm air drier of packing into of airing with clear water, and temperature is in oven dry below 60 ℃, and the baking back packs with bag film.
The invention solves the problem of the technical solutions adopted are: The present invention provides a multi Fringe coca leaf instant tea, is a multi Fringe coca leaf extract was concentrated and dried produce multi-Sui Ke instant tea powder.
Produced according to the invention multi-Sui Ke instant tea, the method comprising the following steps:
a. processing of coca leaf multi Fringe: Fringe will be more coca leaf fixing, second fixing, drying, grinding;
Described completing, 110 ℃-130 ℃ of pot temperature are 8-10 minute from advancing pot to taking the dish out of the pot;
Said secondary completes, and uses drying baker, 110 ℃ of temperature, and leaf-spreading thickness 3-5cm completed 10 minutes;
Said oven dry, 70 ℃ of temperature with the oven dry of Pasania cuspidata leaf, are 5-6 up to water content
%Till;
The crush is the dried crushed leaves of multi-Sui Ke;
b, multi-Sui coca leaf extract: the processed coca leaf multi Fringe ethanol immersion, ultrasonic assisted extraction, filtration, concentration, spray drying and forming;
The extract was weighed and dried coca leaf in accordance with the multi Fringe 1:20 adding 70% ethanol solution soak for 24 hours, at a temperature of ultrasonic assisted 45 ℃, power is 540W extracted under 45 minutes, filtration extraction liquid;
Described concentrate utilizes concentrating under reduced pressure to reclaim ethanol, concentrates 10-20 and carries out ultrafiltration after doubly, carries out continuous centrifugal and filters concentrate with crossing;
Described atomized drying is carried out atomized drying to concentrate, and condition is that feed concentration is about 10%, and EAT is set at 130-140 ℃, and inlet amount is set at 30%;
That described moulding, spray-dired product can be processed into is Powdered, graininess, also can be pressed into various ways such as sheet.
The invention will be more coca leaf by Mizuho fixation, rolling and fried dried, and then the ethanol immersion, ultrasonic assisted extraction, filtration, concentration, spray drying and forming multi-spike coca leaf production of instant tea powder, has the following characteristics: (1) instant Tea is a multi Fringe raw coca leaf, the raw material base for the wild or semi-wild semi-artificial tending from, wide range of sources of raw materials, natural.(2) Multi-Sui Ke instant tea itself contains a lot of flavonoids sweetener, which is 300 times as sweet as sucrose and sweetener itself has high medicinal and health function, which has obvious phlorizin hypoglycemic effect, and high levels of flavonoids has good antioxidant effect, so suitable for different consumer needs.(3) Multi-Sui Ke instant tea production of the mother liquor to extract ethanol for use ultrasonic assisted extraction, can greatly improve the extraction efficiency, thereby possible to ensure active ingredients and nutrients are extracted.This product contains a large amount of flavonoidss, and wherein main component is flavonoidss such as phloridzin, trifloroside and dihydrochalcone, and these three kinds of flavonoidss itself just belong to natural sweetener.This product can be directly and the water allotment drink; Perhaps drink with the allotment of auxiliary material such as other fruit juice, thereby can satisfy different consumers' needs, it meets the requirement of food sanitation safe; Raw material sources are in free of contamination snowy peak mountain range; Belonging to wild resource fully, is a kind of pure drink, belongs to organic food; The product value preserving phase is long, drinks conveniently, meets the demand of modern life rhythm.
Embodiment
Embodiment one,
Fringe Ke picking more leaves.
The preparation method step is following:
1 Fixing: Fixing to promptly picked leaves, so you can ensure maximum content of biologically active substances, when fixing temperature is high, the time as short as possible, for multi-Sui Ke leaves crank up the temperature optimum of 120 ℃, time of 5 minutes.
2 secondary fixation: the use of the oven first temperature to 110 ℃, stalls leaf thickness 3-5cm, crank out more than 10 minutes after the Fringe Ke leaves until the oven temperature dropped to 70 ℃ Fringe Ke leaves baked into the multi- Fringe coca leaf dry up the moisture reaches 5-6% so far.
3 multi Minoru Ko crushed leaves: the use of a dry powder machine Fringe Ke leaves much to smash, smash the degree of 100 mesh.
4. soak: according to solid-to-liquid ratio is that 1:20 added 70% alcohol solution dipping 24 hours.
5. extract: utilizing circulating ultrasonic-assisted extraction is that 45 ℃, power filter after 45 minutes for the condition ultrasonic Extraction of 540W in temperature.
6. concentrate: concentrating under reduced pressure, concentrating degree are 15-20 times, and the continuation continuous centrifugal is crossed and filtered concentrated mother liquor.
7. atomized drying: spray-dired condition, feed concentration are about 10%, and EAT is set at 130-140 ℃, and inlet amount is set at 30%, recovery rate 30%.
8. moulding: process Powdered, graininess, also can be pressed into sheet according to demands of different.
9. pack.
Embodiment two,
Fringe coca leaf picking more.
1? Multiple Fringe coca leaf for the first time fixing: pot temperature 110 ℃ -130 ℃, into the pan to pan from 8-10 minutes;
2 secondary fixation: after the first crank up the multi-Sui Ke leaf drying oven, the temperature at 110 ℃, stalls leaf thickness 3-5cm, fixing 10 minutes;
3 multi Fringe coca leaf crushing: the use of a dry powder machine for grinding many Fringe coca leaf, crushed extent of 100 projects.
4. soak: according to solid-to-liquid ratio is that 1:20 added 70% alcohol solution dipping 24 hours.
5. extract: utilizing circulating ultrasonic-assisted extraction is that 45 ℃, power filter after 45 minutes for the condition ultrasonic Extraction of 540W in temperature.
6. concentrate: concentrating under reduced pressure, concentrating degree are 15-20 times, and the continuation continuous centrifugal is crossed and filtered concentrated mother liquor.
7. atomized drying: spray-dired condition, feed concentration are about 10%, and EAT is set at 130-140 ℃, and inlet amount is set at 30%, recovery rate 30%.
8. moulding: process Powdered, graininess, also can be pressed into sheet according to demands of different.
9. pack.
Claims (1)
1. wild culture technique is imitated in a umbellate pore furgus pure culture manual work, and it is characterized in that: it may further comprise the steps:
(1), choosing ground and whole ground: generally take Schattenseite according to different regions, the Northeast selects the steeper hillside fields of tailo to be advisable, gradient 15-45 degree, promptly except that covering summer the shade, there is drainage condition preferably in the forest land of ground temperature in summer (0.2M is dark) below 25 ℃;
(2), cultivation: make the three-class strain of (umbellate pore furgus is special-purpose) armillaria mellea cultivation bag and umbellate pore furgus prior to seeding about 3 months, to guarantee the high and stable yields of umbellate pore furgus, sowing should be adopted spring sowing, goes back up to 14 ℃ with local ground temperature (0.2M is dark) and is advisable;
(3), the making of armillaria mellea cultivation bag:
A, prescription and medium preparation:
thin wood chip 90%; Wheat bran 10% makes water content reach 55%～56% two kinds of raw material blendings;
B, pack are sterilized, and select the 15*30*0.05cm bag, and sack filling machine (or hand) pack must be adorned reality, and sack is tightened with a mouthful rope folding mouth, and the outer bag of overcoat 17*33*0.04cm carries out normal-pressure sterilization, and (in 500 bags) clocked and kept 24 hours when temperature reached 100 ℃;
C, inoculated and cultured: adopt inoculating hood, semi-solid original seed, the cave inoculation of punching; The good outer bag of cover gets into unglazed training chamber, in the cultivation temperature 15D; Room temperature is controlled at 19～21 ℃, is controlled at 16～19 ℃ to the purseful cultivation temperature behind the 15D and is advisable, and it is on probation that the mycelia purseful promptly can be used for cultivation;
(4), Polyporus strains Production: specific practices: 90% of corn grain, high-rot fine sawdust 10%, through the heart of the corn boil, remove and fine sawdust mix, then add the weight and fine sawdust, etc. water, and then continue Hun mix, marinated that? 750ml bottle or 500ml standard strains of sugar water bottle, sealed immediately after sterilization, sterilization up to 100 ℃ 24h atmospheric pressure above, autoclave reached 0.14 ~ 0.15mPa (depending on container ) 2.5 ~ 3.5h, cooled, and the access Polyporus two kinds, in the light culture room, to 18 ~ 22 ℃ temperature of cultivation to the finished product;
(5), type of seeding:
A, be as the criterion, need 25 bags of armillaria mellea, 2 bottles in umbellate pore furgus three-class strain 750ml bottle (3 bottles in 500ml syrup bottle), fresh thin brush wood filler (diameter 0.02～0.05m, long 0.2m) 20kg, detritus soil (or sandy soil) 0.2m3 with every cave (1 ㎡);
B, go back up to 14 ℃ according to local microclimate ground temperature after, choosing is fine, along sloping gesture excavation 1m, the sowing hole of dark 0.2m; 25 bags of armillaria mellea are sloughed packing and evenly are lying in the hole, are the 0.8m*0.8m area, learn and stay the 0.1m space week, fill up peripheral 0.1m space and bury armillaria mellea bag 1/2 with detritus soil; Umbellate pore furgus bacterial classification (as far as possible being block) is put on the armillaria mellea limit, every bag of both sides are put one to two, fill out with detritus soil not have the umbellate pore furgus bacterial classification again again; Again the fresh inserts of 20kg is evenly put on it, filled out detritus soil while putting, until burying filler; Upward dig above the cave epidermis overlies with former, be turtleback in order to draining, succeeding planting will stay the drain ditch in the ranks in flakes;
(6), management after planting: thermophilic is after planting made regular check on temperature in the cave, must on the cave, add a cover the thing that shades as surpassing 26 ℃, as is lower than 18 ℃ and should adds intense light irradiation; The draining in time when rainy in summer needs on the cave, suitably water low amounts of water (being advisable with enough moisture 0.1-0.15) especially during arid, in addition generally all being main naturally;
(7), management in the coming year: on source points, add a cover the detritus soil of 0.1m, have the crack on the cave or have grey Siberian cocklebur piece to occur as finding behind the rain summer, should continue to add the 0.1m detritus soil again, and other are identical with first year;
(8), management in the 3rd year: along with the conversion of filler nutrition, the increase of Siberian cocklebur piece breeding rate is behind the beginning of spring temperature recovery; Inspection Siberian cocklebur piece is apart from surface distance; It is thick generally once to cover source points ground surface soil 0.15m, and the broad-leaved fallen leaves of 0.1-0.15m are covered in summer has set in again, until gather after autumn;
(9), when ground temperature is reduced to below 14 ℃ after autumn, can excavate, can the superiors' epidermis be removed when excavating, can see the Siberian cocklebur piece; Gradually Siberian cocklebur piece and filler are taken out from one side, the most advanced and sophisticated linen Siberian cocklebur piece of will growing, 1-1.5kg is reserved in every cave; Filling branch material with not rotting fully is embedded in the source points, and other Siberian cocklebur pieces are as the oven dry of commercially available medicine Siberian cocklebur, and most advanced and sophisticated young Siberian cocklebur is cooked kind in the source points plantation; Plant and excavated in back 2 years, the old cave after the plantation can not be planted continuously again, needs to plant after 2 years at interval again;
(10), drying processing: the bright Siberian cocklebur that will dig is back cleaned one day warm air drier of packing into of airing with clear water, and temperature is in oven dry below 60 ℃, and the baking back packs with bag film.
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| CN104322280A (en) * | 2014-10-23 | 2015-02-04 | 赵俊瑞 | Culture method for tuber edible fungi |
| CN104350943A (en) * | 2014-10-23 | 2015-02-18 | 赵俊瑞 | Method for cultivating edible fungi |
| CN105075660A (en) * | 2015-01-29 | 2015-11-25 | 张世定 | Method of wild-growth-imitation planting of polyporus umbellatus in Pu-er mountainous area |
| CN105230340A (en) * | 2015-10-08 | 2016-01-13 | 贵州省习水县药材公司 | Three-dimensional composite planting method for gastrodia elata and polyporus under wood |
| CN104170652B (en) * | 2014-08-21 | 2016-06-01 | 两当县云屏乡裕通猪苓产销专业合作社 | A kind of utilize the method filling segment wood cultivated umbellate pore furgus |
| CN108575548A (en) * | 2018-01-31 | 2018-09-28 | 云南省农业科学院高山经济植物研究所 | A kind of implantation methods of NW YUNNAN umbellate pore furgus stable high yield |
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