CN103570422A - Edible fungi particle strain formula and production technology - Google Patents
Edible fungi particle strain formula and production technology Download PDFInfo
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- CN103570422A CN103570422A CN201310549967.0A CN201310549967A CN103570422A CN 103570422 A CN103570422 A CN 103570422A CN 201310549967 A CN201310549967 A CN 201310549967A CN 103570422 A CN103570422 A CN 103570422A
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Abstract
The invention discloses an edible fungi particle strain formula and production technology. According to the formula, a strain culture medium comprises the following components: 97.5% of sorghum grains, 1.5% of light calcium carbonate and 1% of glucose. The production technology comprises the steps of selecting materials, rinsing, soaking, cooking, removing free water, stirring, bottling, plugging, packaging, tying, carrying out high-pressure sterilization, discharging, inoculating, culturing mycelium, shaking a bottle for the first time, shaking the bottle for the second time to obtain a finished product of the strain. According to the production technology, a container bottle with a small bottle mouth is used as a packing material of the strain, and a tampon used for sealing is covered by a thin film, so that the demand of mycelium which grows in the container bottle on the oxygen can be met, and the pollution of sundry fungus to the strain can be reduced; a technology of shaking the bottle for two times is adopted, so that the bottle filling speed of the mycelium is increased by more than double, and the fungus ages at the upper part and the lower part are consistent with each other when the mycelium is fully filled into the bottle. The strain produced by using the production technology is high in purity, high in inoculation speed, fast in germination and good in feeding, is deeply popularized by vast mushroom farmers.
Description
Technical field
The present invention relates to a kind of formula and production technique thereof of edible fungus granules bacterial classification.
Background technology
The formula of tradition edible mushrooms relates generally to more composition and production technique is conventionally used pack tying or used large mouthful Cans, the method for upper cover kraft paper sealing.Facts have proved, adopt the mouth-sealing method of these two kinds of techniques all to have drawback, if tying is too tight, easily cause a bottle interior mycelia anoxic, mycelial growth rate is slow and sparse; If tying is loose, be easy to cause living contaminants.Provide a kind of simple edible mushrooms formula with conventional formulation and our plan of technique comparison, its production technique not only can meet the interior mycelial growth of container bottle to the demand of oxygen and can reduce living contaminants.
Summary of the invention
The object of the invention is for a kind of edible fungus granules bacterial classification formula and production technique are provided, the finished product bacterial classification that this invention is produced is pure culture flowable particulates bacterial classification, its production technique not only can meet the demand of mycelial growth to oxygen in container bottle, can also reduce living contaminants.
The present invention is achieved through the following technical solutions:
A kind of edible fungus granules bacterial classification formula, is characterized in that its formula is composed of the following components: sorghum grain 97.5%, light calcium carbonate 1.5%, glucose 1%.
Its technical process is: select materials → rinsing → immersion → boiling → removal free-water → spice → bottling → plug mouth → bag mouth, tying → autoclaving → taking the dish out of the pot, inoculate → mycelium culture → mono-time shaking flask → secondary shaking flask → finished product bacterial classification.
The production technique of described edible fungus granules bacterial classification formula, is characterized in that its concrete technology step is as follows:
(1) select materials: strictly select and be neither with shell, again unabroken sorghum grain;
(2) rinsing: by sorghum grain clear water rinsing, remove the shell skin, dust and other impurity that are mingled with;
(3) soak: the clean sorghum grain of rinsing soaks 5 ~ 6 hours with clear water, makes it suction moisture;
(4) boiling: the in-built tap water of pot, burn greatly while just having emitted bubble to water, soaked sorghum grain to be poured in pot interior water, the water yield will be soaked sorghum grain 4cm; Large baked wheaten cake to boiling in pot 18 ~ 20 minutes, treats that sorghum grain boils to hand, to pinch flatly, but must not break grain, drags for into bamboo basket, drains moisture;
(5) remove free-water: with gas blower, the free-water on above-mentioned sorghum grain surface is dried up;
(6) spice: press formula rate, admix light calcium carbonate and glucose, make each sorghum grain evenly be stained with light calcium carbonate, and between particle, mutual adhesion is good;
(7) bottling: the sorghum grain of mixing is packed in 500ml saline bottle, loading amount account for bottle long-pending 3/5ths;
(8) plug mouthful: the bottleneck that installs Chinese sorghum is made into tampon with old cotton, blocking bottleneck after wiping clean with clean-cloth;
(9) bag mouthful, tying: bottleneck tampon is wrapped with single-layer polypropylene film, and by plastic ties, bottleneck is wrapped up solidly, to prevent that tampon from being drenched by steam in sterilization process, can farthest reduce and drench the living contaminants causing because of tampon;
(10) autoclaving: above-mentioned bottle packs pressure kettle into, drains after freezing air, with the pressure of 0.0147Mpa, keeps 2 hours;
(11) take the dish out of the pot, inoculate: treat that gauge hand returns to " zero ", above-mentioned bottle is taken out in pot, put into aseptic inoculation box or Bechtop, aseptic technique inoculation;
(12) mycelium culture: postvaccinal seed bottle is put on the bacterial classification frame of culturing room and carried out mycelium culture;
(13) shaking flasks: after inoculation, cultivate under normal temperature 5 days, when mycelia grows to the agglomerate of egg size, carry out shaking flask for the first time; Method: the hypha body that grows to egg size is clapped and fallen apart, and in bottle, a culture material shakes up up and down;
(14) secondary shaking flask: after shaking flask 5 days for the first time, mycelia is substantially full bottle up and down, then carries out shaking flask for the second time; Method: all sorghum grains are clapped and fallen apart, mix up and down;
(15) finished product bacterial classification: after secondary shaking flask 3 ~ 4 days, dense, sturdy, pure white mycelia was covered with bottle, when aerial hyphae approximately has 2 ~ 3mm long, can be used.
With respect to prior art, the invention has the beneficial effects as follows:
1) with saline bottle, make production of hybrid seeds container, its bottleneck is little and upright degree good, easy-to-use tampon sealing, and as the female kind of making pure culture, tampon can play and both filter external miscellaneous bacteria, meets again the demand of the interior mycelial growth of bottle to oxygen.
2) bottleneck tampon is wrapped with single-layer polypropylene film, and by plastic ties, bottleneck is wrapped up solidly, to prevent that tampon from being drenched by steam in sterilization process, can farthest reduce and drench the living contaminants causing because of tampon.
3) light calcium carbonate in formula can play and both supplement the required calcium nutrition of mycelial growth, does not make again effect inter-adhesive between sorghum grain.Even strain aging, the sorghum grain in bacterial classification also can stir into particulate state, and can not affect the sprouting of the inner mycelia of sorghum grain, take and guarantees that the bacterial classification of producing is pure culture granular bacteria strain.
4) glucose in formula can be mycelia direct nutrient is provided, and makes that mycelium germination is fast, field planting is fast.
5) this technique has increased by twice shaking flask in making processes, can make the full bottle of mycelia speed add fast again more than, and upper and lower cell age is consistent during the full bottle of mycelia.
6) through production practice for many years, prove, this bacterial classification purity is high, inoculation speed fast, it is fast to sprout, material feeding is good, welcome by vast mushroom agriculture.
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated.
embodiment:
1, a kind of edible fungus granules bacterial classification formula: sorghum grain 97.5%, light calcium carbonate 1.5%, glucose 1%.
2, its technical process is:
Select materials → rinsing → immersion → boiling → removal free-water → spice → bottling → plug mouth → bag mouth, tying → autoclaving → taking the dish out of the pot, inoculate → mycelium culture → mono-time shaking flask → secondary shaking flask → finished product bacterial classification.
3, main production comprises:
(1) select materials: strictly select and be neither with shell, again unabroken sorghum grain.
(2) rinsing: by sorghum grain clear water rinsing, remove the shell skin, dust and other impurity that are mingled with.
(3) soak: the clean sorghum grain of rinsing soaks 5 ~ 6 hours with clear water, makes it suction moisture.
(4) boiling: the in-built tap water of pot, burn greatly while just having emitted bubble to water, soaked sorghum grain to be poured in pot interior water, the water yield will be soaked sorghum grain 3cm left and right.Large baked wheaten cake to boiling in pot 18 ~ 20 minutes, treats that sorghum grain boils to hand, to pinch flatly, but must not break grain (i.e. cracking), drags for into bamboo basket, drains moisture.
(5) remove free-water: with gas blower, the free-water on above-mentioned sorghum grain surface is dried up.
(6) spice: by specified mix in patent, admix light calcium carbonate and glucose: make each sorghum grain evenly be stained with light calcium carbonate, and between particle, mutual adhesion is good.
(7) bottling: the sorghum grain of mixing is packed in 500ml saline bottle, and loading amount accounts for 3/5ths long-pending (quantitative with autocratic measuring device) of bottle.Can't pack too much an inhomogeneous and bacterium anoxic while avoiding shaking flask into.
(8) plug mouthful: the bottleneck that installs Chinese sorghum is made into tampon with old cotton, blocking bottleneck after wiping clean with clean-cloth.(tampon can play and both filter miscellaneous bacteria, the effect of supplying again oxygen, its mycelia purity is to female standard of planting pure culture).
(9) bag mouthful, tying: bottleneck tampon is wrapped with single-layer polypropylene film, and by plastic ties, bottleneck is wrapped up solidly, to prevent that tampon from being drenched by steam in sterilization process, can farthest reduce and drench the living contaminants causing because of tampon.
(10) autoclaving: above-mentioned bottle packs pressure kettle into, drains after freezing air, with the pressure of 0.0147Mpa, keeps 2 hours.
(11) take the dish out of the pot, inoculate: treat that gauge hand returns to " zero ", above-mentioned bottle is taken out in pot, put into aseptic inoculation box or Bechtop, aseptic technique inoculation.
(12) mycelium culture: postvaccinal seed bottle is put on the bacterial classification frame of culturing room and carried out mycelium culture.
(13) shaking flasks: after inoculation, under normal temperature, (general 15 ℃ ~ 25 ℃ all can) be cultivated 5 days, when mycelia grows to the agglomerate of egg size, carries out shaking flask for the first time.Method: the hypha body that grows to egg size is clapped and fallen apart, and in bottle, a culture material shakes up up and down.
(14) secondary shaking flask: after shaking flask 5 days for the first time, mycelia is substantially full bottle up and down, then carries out shaking flask for the second time.Method: all sorghum grains are clapped and fallen apart, mix up and down.
(15) finished product bacterial classification: after secondary shaking flask 3 ~ 4 days, dense, sturdy, pure white mycelia was covered with bottle, when aerial hyphae approximately has 2 ~ 3mm long, can be used.
Using method of the present invention:
(1) sorghum grain that covers with mycelia in bottle is clapped and fallen apart, make it into mutual NA particulate state, now mycelia sees not obvious (mycelia of sorghum grain inside is still dense intact) in appearance.The bacterium bag being vaccinated is opened, and jowar particle directly rolls and flows out from bottleneck, about 25 left and right, and make it to be evenly distributed on sack, finally prick upper bag mouth and inoculate complete.Postvaccinal sorghum grain bacterial classification can sprout the pure white mycelia as cotton mass after 24 hours, and material feeding is respond well.
(2) if in bottle the mycelia time slightly long, the sorghum grain that covers with mycelia should not directly be clapped loose, the inoculation hook after available flame disinfection stirs sorghum grain, makes it to be shattered into particulate state.The bacterium bag being vaccinated is opened, and jowar particle directly rolls and flows out from bottleneck, about 25 left and right, and make it to be evenly distributed on sack, finally prick upper bag mouth and inoculate complete.Postvaccinal sorghum grain bacterial classification can sprout the pure white mycelia as cotton mass after 24 hours, and material feeding is respond well.
(3) one bottles of bacterial classifications can connect bacterium bag: 60～65 bags of one end inoculations; If two ends inoculation, can connect 30～35 bags.
(4) easily and fast, success ratio of inoculation can reach more than 98% in this bacterial classification inoculation.
Claims (3)
1. an edible fungus granules bacterial classification formula, is characterized in that its formula is composed of the following components: sorghum grain 97.5%, light calcium carbonate 1.5%, glucose 1%.
2. the production technique of edible fungus granules bacterial classification formula according to claim 1, is characterized in that its technical process is: select materials → rinsing → immersion → boiling → removal free-water → spice → bottling → plug mouth → bag mouth, tying → autoclaving → taking the dish out of the pot, inoculate → mycelium culture → mono-time shaking flask → secondary shaking flask → finished product bacterial classification.
3. the production technique that edible fungus granules bacterial classification according to claim 2 is filled a prescription, is characterized in that its concrete technology step is as follows:
(1) select materials: strictly select and be neither with shell, again unabroken sorghum grain;
(2) rinsing: by sorghum grain clear water rinsing, remove the shell skin, dust and other impurity that are mingled with;
(3) soak: the clean sorghum grain of rinsing soaks 5 ~ 6 hours with clear water, makes it suction moisture;
(4) boiling: the in-built tap water of pot, burn greatly while just having emitted bubble to water, soaked sorghum grain to be poured in pot interior water, the water yield will be soaked sorghum grain 4cm; Large baked wheaten cake to boiling in pot 18 ~ 20 minutes, treats that sorghum grain boils to hand, to pinch flatly, but must not break grain, drags for into bamboo basket, drains moisture;
(5) remove free-water: with gas blower, the free-water on above-mentioned sorghum grain surface is dried up;
(6) spice: press formula rate, admix light calcium carbonate and glucose, make each sorghum grain evenly be stained with light calcium carbonate, and between particle, mutual adhesion is good;
(7) bottling: the sorghum grain of mixing is packed in 500ml saline bottle, loading amount account for bottle long-pending 3/5ths;
(8) plug mouthful: the bottleneck that installs Chinese sorghum is made into tampon with old cotton, blocking bottleneck after wiping clean with clean-cloth;
(9) bag mouthful, tying: bottleneck tampon is wrapped with single-layer polypropylene film, and by plastic ties, bottleneck is wrapped up solidly, to prevent that tampon from being drenched by steam in sterilization process, can farthest reduce and drench the living contaminants causing because of tampon;
(10) autoclaving: above-mentioned bottle packs pressure kettle into, drains after freezing air, with the pressure of 0.0147Mpa, keeps 2 hours;
(11) take the dish out of the pot, inoculate: treat that gauge hand returns to " zero ", above-mentioned bottle is taken out in pot, put into aseptic inoculation box or Bechtop, aseptic technique inoculation;
(12) mycelium culture: postvaccinal seed bottle is put on the bacterial classification frame of culturing room and carried out mycelium culture;
(13) shaking flasks: after inoculation, cultivate under normal temperature 5 days, when mycelia grows to the agglomerate of egg size, carry out shaking flask for the first time; Method: the hypha body that grows to egg size is clapped and fallen apart, and in bottle, a culture material shakes up up and down;
(14) secondary shaking flask: after shaking flask 5 days for the first time, mycelia is substantially full bottle up and down, then carries out shaking flask for the second time; Method: all sorghum grains are clapped and fallen apart, mix up and down;
(15) finished product bacterial classification: after secondary shaking flask 3 ~ 4 days, dense, sturdy, pure white mycelia was covered with bottle, when aerial hyphae approximately has 2 ~ 3mm long, can be used.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1043610A (en) * | 1989-03-03 | 1990-07-11 | 福建农学院 | Make the technology of nutritional solution with the JINZHENGU solid culture |
| EP0564857A1 (en) * | 1992-04-03 | 1993-10-13 | MAYKO ENTSORGUNGSBETRIEBE GmbH | Plant growth substrate with fertilising effect, in particulate free flowing form and process for the manufacture thereof |
| CN1666589A (en) * | 2004-12-15 | 2005-09-14 | 田绍义 | Bacterial strain isolation and cultivation technique for Chinese wild species big fat mushroom |
| CN101637101A (en) * | 2009-08-19 | 2010-02-03 | 蛟河市黑土白云食用菌有限公司 | Industrial cultivation method of selenium-enriched agaricus bisporus |
| CN101849478A (en) * | 2010-02-22 | 2010-10-06 | 北京农学院 | Coprinus atramentarius and cultivation method thereof |
-
2013
- 2013-11-08 CN CN201310549967.0A patent/CN103570422B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1043610A (en) * | 1989-03-03 | 1990-07-11 | 福建农学院 | Make the technology of nutritional solution with the JINZHENGU solid culture |
| EP0564857A1 (en) * | 1992-04-03 | 1993-10-13 | MAYKO ENTSORGUNGSBETRIEBE GmbH | Plant growth substrate with fertilising effect, in particulate free flowing form and process for the manufacture thereof |
| CN1666589A (en) * | 2004-12-15 | 2005-09-14 | 田绍义 | Bacterial strain isolation and cultivation technique for Chinese wild species big fat mushroom |
| CN101637101A (en) * | 2009-08-19 | 2010-02-03 | 蛟河市黑土白云食用菌有限公司 | Industrial cultivation method of selenium-enriched agaricus bisporus |
| CN101849478A (en) * | 2010-02-22 | 2010-10-06 | 北京农学院 | Coprinus atramentarius and cultivation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王尚荣: "白灵菇富硒栽培技术研究", 《食用菌学报》 * |
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Inventor after: Wang Shangrong Inventor after: Zhou Tianhua Inventor after: Yu Zhangyu Inventor after: Zhang Yuanhao Inventor after: Wang Juan Inventor after: Tian Geilin Inventor after: Liu Gaofeng Inventor after: Sun Lei Inventor before: Wang Shangrong Inventor before: Yu Zhangyu Inventor before: Zhou Tianhua Inventor before: Zhang Yuanhao Inventor before: Wang Juan Inventor before: Tian Geilin Inventor before: Liu Gaofeng Inventor before: Sun Lei |
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Effective date of registration: 20151216 Address after: 274015 School of life science, 2269 College Road, Mudan District, Heze, Shandong Applicant after: Heze University Address before: 274015, Heze University, 2269, University Road, Shandong, Heze Applicant before: Wang Shangrong |
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Granted publication date: 20160203 Termination date: 20191108 |