CN102613169A - Method for prolonging preservation period of rice leafroller eggs - Google Patents
Method for prolonging preservation period of rice leafroller eggs Download PDFInfo
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- CN102613169A CN102613169A CN2012101250950A CN201210125095A CN102613169A CN 102613169 A CN102613169 A CN 102613169A CN 2012101250950 A CN2012101250950 A CN 2012101250950A CN 201210125095 A CN201210125095 A CN 201210125095A CN 102613169 A CN102613169 A CN 102613169A
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Abstract
The invention provides a method for prolonging the preservation period of rice leafroller eggs. The invention provides an egg preservation method which can firstly implement progressive cooling training on the rice leafroller eggs, then keep for a long time at the temperature of 15 DEG C and further perform progressive heating activation against the problem that rice leafrollers can not be kept with high efficiency for the long time during indoor rearing. The method is characterized in that the method comprises the following steps of: keeping the eggs laid at the temperature of 25 DEG C at 23 DEG C, 21 DEG C, 19 DEG C and 17 DEG C for 2 hours, 4 hours, 8 hours and 10 hours respectively, then placing at 15 DEG C, preserving for a certain number of days, further sequentially keeping the eggs at 17 DEG C, 19 DEG C, 21 DEG C and 23 DEG C for 2 hours, 4 hours, 8 hours and 10 hours respectively, and then hatching the eggs at 25 DEG C. According to the method disclosed by the invention, the eggs can be effectively preserved for 22 days, and the hatching rate is 75% of that at the normal temperature of 25 DEG C. According to the method disclosed by the invention, the flexibility in rearing of the rice leafrollers can be effectively improved, the cost can be reduced, and powerful guarantee can be provided for smooth development of test research of the rice leafrollers.
Description
Technical field
The invention belongs to insect and raise the field, be specifically related to a kind of method that prolongs rice leaf roller ovum storage life.
Background technology
Rice leaf roller is a kind of important pests on the paddy rice; Take place comparatively serious in recent years; Bring loss to paddy rice production; Therefore study of adaptations, development of drug resistance and the population genetic variation etc. of this worm, disclose the population regularity of occurrence and development, thereby provide the guidance etc. of theoretical and method to seem quite important for the control management each envirment factor.Above-mentioned research is mostly carried out indoor, and rice leaf roller indoor successive raising efficiently for a long time is the important leverage that conducts a research smoothly.Indoor feeding about rice leaf roller; The existing existing method of utilizing paddy rice, maize seedling and artificial feed to raise; But all do not solve the long-time efficient problem of preserving of rice leaf roller ovum in these raising method, institute's fresh ovum that produces normally can only preserved 3-5 days under the raising temperature.And if can preserve the rice leaf roller ovum for a long time, this will improve the availability of raising insect greatly, strengthen the flexibility of feeding time regulation and control, thereby reduce feeding cost, accelerate the research progress.Temperature is to influence the key factor that insect grows, and low temperature can slow down the development progress of insect usually, and still the survival rate of insect or the incubation rate of ovum also can fall sharply at low temperatures.Therefore, the needs invention is a kind of can to prolong the rice leaf roller ovum holding time, can guarantee the method than high hatchability again.
Summary of the invention
The purpose of this invention is to provide a kind of method that prolongs rice leaf roller ovum storage life.Through the rice leaf roller ovum is carried out alternating temperature processing step by step before and after low temperature is preserved, with storage life that prolongs ovum and the high hatchability that guarantees ovum.
The objective of the invention is to reach through following method:
The following rice leaf roller ovum that produces of normal raising temperature is carried out the training of low temperature step by step of short-term earlier; Then preserve the long period at low temperatures; And then the cascade raising temperature that carries out short-term activates, under normal temperature, hatch again at last, thereby significant prolongation the storage life of ovum.
Effect of the present invention:
Method of the present invention is compared with existing rice leaf roller ovum normal temperature store method, produces following beneficial effect: (1) is through alternating temperature step by step and preserve at low temperatures, significant prolongation ovum storage life and do not influence the hatching of ovum.(2) can improve the availability that rice leaf roller is raised the examination worm, for the experimental study of this worm provides sufficient worm source.(3) can regulate the examination worm easily and flexibly and raise progress, guarantee the economy and the continuity of raising.
Embodiment
The inventive method can realize by following mode, but be not limited to this mode.
The low temperature training condition: to the rice leaf roller ovum that following the same night produced at 25 ℃, be put in the bottom and be lined with in the translucent plastic beaker of wet water absorbent paper, cover the beaker mouth with gauze then, 7:30 changes in 23 ℃ of illumination boxs and kept 2 hours the morning; Change over to then in 21 ℃ of illumination boxs and kept 4 hours; Then change in 19 ℃ of illumination boxs and kept 8 hours; Change 17 ℃ in 21:30 at last and kept 10 hours down, 7:30 forwards under 15 ℃ the next morning.
The condition that low temperature is preserved: the ovum after the low temperature training is put in and keeps 5 days, 10 days, 15 days, 20 days, 25 days and 30 days in the illumination box under 15 ℃ respectively.
Heat up and activate the condition of ovum hatching: after keeping certain fate under 15 ℃; The ovum of 7:30 after with low temperature treatment forwards 17 ℃ to and kept 2 hours down in the morning; Place 19 ℃ to keep 4 hours, 21 ℃ to keep 8 hours, 23 ℃ to keep 10 hours down down down then successively, place at last under 25 ℃ and hatch.
By the above-mentioned low temperature preservation condition method that relents step by step respectively to the processing of lowering the temperature step by step earlier of 300 above ovum; Under 15 ℃, carry out 5-30 days preservation then; Then carrying out cascade raising temperature handles; Measure its incubation rate under 25 ℃ at last, with normal raising temperature promptly 25 ℃ of ovum of preserving down also promptly directly put into 15 ℃ of ovum of preserving 17 days, 22 days and 24 days simultaneously and contrast as over against photograph for negative by 25 ℃ with alternating temperature suddenly.
The illumination condition of above-mentioned processing is illumination every day 14 hours, and incubator 6:00 every day turns on light, and 20:00 turns off the light.
Implementation result:
The hatching of rice leaf roller ovum research shows (table 1) under the different preservation conditions, through low temperature training step by step, low temperature preserve and step by step activated at can effectively preserve worm's ovum.Under 14 hours every days illumination conditions, alternating temperature is handled and is preserved back ovum storage life with low temperature and be 17 days step by step, and this moment, the incubation rate of ovum was compared with the incubation rates of 25 ℃ of normal down raising ovum, no significant difference, and 25 ℃ of following ovum can be preserved 3 days.When but low temperature was preserved 17 days behind the alternating temperature suddenly, egg hatching rate significantly was lower than the contrast of normal raising.Alternating temperature processing and low temperature were preserved after 22 days step by step; Though compared significant reduction under the hatching of ovum and the normal raising; But it still is 75% under normal the raising, and alternating temperature is preserved after 22 days down suddenly, and the incubation rate of ovum is extremely low; Be merely about 2% under the normal raising, two kinds of processing mode significant differences (seeing table 1 for details).
This method shows that the holding time of rice leaf roller ovum under illumination in 14 hours can extend to 22 days after implementing.
Incubation rate and the ratio that contrasts 25 ℃ of following incubation rates after the table 1 ovum preservation different number of days
| Processing mode | The preservation fate (my god) | Incubation rate and the ratio of contrast under 25 ℃ |
| CK | 3 | 1.0a |
| Alternating temperature step by step | 7 | 1.06±0.20a |
| 12 | 0.95±0.21ab | |
| 17 | 0.89±0.20ab | |
| 22 | 0.75±0.26b | |
| 27 | 0.01±0.01c | |
| 32 | 0.03±0.04c | |
| Suddenly alternating temperature | 17 | 0.76±0.10b |
| 22 | 0.02±0.02c | |
| 24 | 0.0c |
Notes: alternating temperature preservation fate is to preserve fate under 15 ℃ to add cooling processing step by step 1 day (promptly 24 hours) and 1 day (promptly 24 hours) of cascade raising temperature processing step by step; It is remarkable that different lowercases are represented to preserve between fate the incubation rate ratio difference.
Claims (4)
1. a method that prolongs rice leaf roller ovum storage life is characterized in that the rice leaf roller ovum of 25 ℃ of raisings is carried out preserving at low temperatures after the low temperature treatment step by step, and then hatches under cascade raising temperature to the normal temperature, has prolonged the ovum storage life.
2. rice leaf roller ovum according to claim 1 carries out low temperature treatment step by step, and warp kept respectively 2 hours, 4 hours, 8 hours and 10 hours for 23 ℃, 21 ℃, 19 ℃ and 17 ℃ to it is characterized in that laying eggs successively at 25 ℃ following the rice leaf roller adult.
3. rice leaf roller ovum according to claim 1 is preserved at low temperatures, it is characterized in that the rice leaf roller ovum is preserved down at 15 ℃.
4. rice leaf roller ovum cascade raising temperature according to claim 1 is characterized in that the rice leaf roller ovum was kept respectively 2 hours, 4 hours, 8 hours and 10 hours through 17 ℃, 19 ℃, 21 ℃ and 23 ℃ successively.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210125095.0A CN102613169B (en) | 2012-04-26 | 2012-04-26 | Method for prolonging preservation period of rice leafroller eggs |
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| CN201210125095.0A CN102613169B (en) | 2012-04-26 | 2012-04-26 | Method for prolonging preservation period of rice leafroller eggs |
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| CN102613169A true CN102613169A (en) | 2012-08-01 |
| CN102613169B CN102613169B (en) | 2014-06-11 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638580A (en) * | 2016-02-26 | 2016-06-08 | 珠海友木友科技有限公司 | Refrigeration preservation method for hermetia illucens prepupa |
| CN105724365A (en) * | 2016-02-26 | 2016-07-06 | 珠海友木友科技有限公司 | Black soldier fly egg refrigeration preservation method |
| CN112997972A (en) * | 2021-03-30 | 2021-06-22 | 河南省农业科学院烟草研究所 | Ornitus avidus egg preservation method suitable for large-scale breeding |
| WO2021145760A1 (en) * | 2020-01-17 | 2021-07-22 | Protix B.V. | Method for controlling hatching of black soldier fly eggs |
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| JPH01233266A (en) * | 1988-03-11 | 1989-09-19 | Sumitomo Chem Co Ltd | Benzoylurea derivative, production thereof and insecticide containing said derivative as active ingredient |
| WO1995018116A1 (en) * | 1993-12-29 | 1995-07-06 | E.I. Du Pont De Nemours And Company | Arthropodicidal oxadiazine-, thiadiazine- or triazine-carboxanilides |
| CN101796939A (en) * | 2010-02-25 | 2010-08-11 | 浙江省农业科学院 | Egg laying device of cnaphalocrocis medinalis guenee and use method thereof |
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2012
- 2012-04-26 CN CN201210125095.0A patent/CN102613169B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01233266A (en) * | 1988-03-11 | 1989-09-19 | Sumitomo Chem Co Ltd | Benzoylurea derivative, production thereof and insecticide containing said derivative as active ingredient |
| WO1995018116A1 (en) * | 1993-12-29 | 1995-07-06 | E.I. Du Pont De Nemours And Company | Arthropodicidal oxadiazine-, thiadiazine- or triazine-carboxanilides |
| CN101796939A (en) * | 2010-02-25 | 2010-08-11 | 浙江省农业科学院 | Egg laying device of cnaphalocrocis medinalis guenee and use method thereof |
Non-Patent Citations (2)
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| 张士昶 等: "南方小花蝽对寄主植物的产卵选择性及其卵的保存条件", 《昆虫知识》, vol. 45, no. 4, 30 April 2008 (2008-04-30), pages 600 - 603 * |
| 李道生: "一种延长大劣按蚊虫卵保存时间的方法", 《中国寄生虫学与寄生虫病杂志》, vol. 21, no. 5, 31 October 2003 (2003-10-31), pages 278 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638580A (en) * | 2016-02-26 | 2016-06-08 | 珠海友木友科技有限公司 | Refrigeration preservation method for hermetia illucens prepupa |
| CN105724365A (en) * | 2016-02-26 | 2016-07-06 | 珠海友木友科技有限公司 | Black soldier fly egg refrigeration preservation method |
| WO2021145760A1 (en) * | 2020-01-17 | 2021-07-22 | Protix B.V. | Method for controlling hatching of black soldier fly eggs |
| CN112997972A (en) * | 2021-03-30 | 2021-06-22 | 河南省农业科学院烟草研究所 | Ornitus avidus egg preservation method suitable for large-scale breeding |
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| CN102613169B (en) | 2014-06-11 |
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