CN102613169B

CN102613169B - Method for prolonging preservation period of rice leafroller eggs

Info

Publication number: CN102613169B
Application number: CN201210125095.0A
Authority: CN
Inventors: 刘向东; 廖怀建; 黄建荣; 方源松
Original assignee: Nanjing Agricultural University
Current assignee: Nanjing Agricultural University
Priority date: 2012-04-26
Filing date: 2012-04-26
Publication date: 2014-06-11
Anticipated expiration: 2032-04-26
Also published as: CN102613169A

Abstract

The invention provides a method for prolonging the preservation period of rice leafroller eggs. The invention provides an egg preservation method which can firstly implement progressive cooling training on the rice leafroller eggs, then keep for a long time at the temperature of 15 DEG C and further perform progressive heating activation against the problem that rice leafrollers can not be kept with high efficiency for the long time during indoor rearing. The method is characterized in that the method comprises the following steps of: keeping the eggs laid at the temperature of 25 DEG C at 23 DEG C, 21 DEG C, 19 DEG C and 17 DEG C for 2 hours, 4 hours, 8 hours and 10 hours respectively, then placing at 15 DEG C, preserving for a certain number of days, further sequentially keeping the eggs at 17 DEG C, 19 DEG C, 21 DEG C and 23 DEG C for 2 hours, 4 hours, 8 hours and 10 hours respectively, and then hatching the eggs at 25 DEG C. According to the method disclosed by the invention, the eggs can be effectively preserved for 22 days, and the hatching rate is 75% of that at the normal temperature of 25 DEG C. According to the method disclosed by the invention, the flexibility in rearing of the rice leafrollers can be effectively improved, the cost can be reduced, and powerful guarantee can be provided for smooth development of test research of the rice leafrollers.

Description

A kind of method that extends Eggs of The Rice Leaf Roller storage life

Technical field

The invention belongs to insect and raise field, be specifically related to a kind of method that extends Eggs of The Rice Leaf Roller storage life.

Background technology

Rice leaf roller is a kind of important pests on paddy rice, occur in recent years comparatively serious, bring loss to Rice Production, therefore study the genetic variation of adaptations, development of drug resistance and the population of this worm to each envirment factor etc., disclose population regularity of occurrence and development, thereby seem quite important for control management provides the guidance etc. of theoretical and method.Above-mentioned research is mostly carried out indoor, and it is the important leverage conducting a research smoothly that the long-term efficient indoor subculture of rice leaf roller is raised.About the indoor feeding of rice leaf roller, the now existing method of utilizing paddy rice, maize seedling and artificial feed to raise, but the long-time efficient problem of preserving that does not all solve Eggs of The Rice Leaf Roller in these raising method, institute's fresh ovum that produces normally can only preserved 3-5 days under raising temperature.And if can preserve for a long time Eggs of The Rice Leaf Roller, this will greatly improve to raise insect availability, strengthen the flexibility of feeding time regulation and control, thereby reduce feeding cost, accelerate research progress.Temperature is to affect the key factor that insect grows, and low temperature can slow down the development progress of insect conventionally, and still the survival rate of insect or the incubation rate of ovum also can fall sharply at low temperatures.Therefore, need to invention one the Eggs of The Rice Leaf Roller holding time can be extended, the method compared with high hatchability can be guaranteed again.

Summary of the invention

The object of this invention is to provide a kind of method that extends Eggs of The Rice Leaf Roller storage life.By Eggs of The Rice Leaf Roller is carried out to alternating temperature processing step by step before and after low temperature is preserved, to extend the storage life of ovum and the high hatchability of assurance ovum.

The object of the invention is to reach by the following method:

Lower normal raising temperature the Eggs of The Rice Leaf Roller that produces is first carried out to the training of low temperature step by step of short-term, then preserve the long period at low temperatures, and then carry out short-term cascade raising temperature activate, finally under normal temperature, hatch again, thus significant prolongation the storage life of ovum.

Effect of the present invention:

Method of the present invention is compared with existing Eggs of The Rice Leaf Roller normal temperature store method, produces following beneficial effect: (1) is by alternating temperature step by step and preserve at low temperatures, significant prolongation ovum storage life and do not affect the hatching of ovum.(2) can improve rice leaf roller and raise the availability of trying worm, for the experimental study of this worm provides sufficient worm source.(3) can regulate easily and flexibly examination worm to raise progress, guarantee economy and the continuity of raising.

Embodiment

The inventive method can realize in the following manner, but is not limited to this mode.

Low temperature training condition: to the Eggs of The Rice Leaf Roller that the same night produces at 25 ℃, be put in bottom and be lined with in the translucent plastic beaker of wet water absorbent paper, then cover beaker mouth with gauze, 7:30 proceeds in 23 ℃ of illumination boxs and keeps 2 hours the morning; Then proceed in 21 ℃ of illumination boxs and keep 4 hours; Then proceed in 19 ℃ of illumination boxs and keep 8 hours; Finally proceed at 17 ℃ and keep 10 hours in 21:30, the next morning, 7:30 forwards at 15 ℃.

The condition that low temperature is preserved: the ovum after low temperature training is put in the illumination box at 15 ℃ and keeps respectively 5 days, 10 days, 15 days, 20 days, 25 days and 30 days.

Heat up and activate the condition of ovum hatching: at 15 ℃, keep after certain number of days, 7:30 forwards the ovum after low temperature treatment at 17 ℃ to and keeps 2 hours in the morning, then be placed in successively at 19 ℃ and keep keeping keeping 10 hours at 8 hours, 23 ℃ at 4 hours, 21 ℃, be finally placed at 25 ℃ and hatch.

By the above-mentioned low temperature preservation condition method that relents step by step respectively to the processing of first lowering the temperature step by step of more than 300 ovum, then at 15 ℃, carry out the preservation of 5-30 days, then carry out cascade raising temperature processing, finally measure its incubation rate at 25 ℃, the ovum of preserving at 25 ℃ using normal raising temperature is as positive control, also directly puts into 15 ℃ of ovum of preserving 17 days, 22 days and 24 days by 25 ℃ take alternating temperature suddenly simultaneously and contrasts for negative.

The illumination condition of above-mentioned processing is illumination every day 14 hours, and incubator 6:00 every day turns on light, and 20:00 turns off the light.

Implementation result:

Under different preservation conditions, the hatching of Eggs of The Rice Leaf Roller research shows (table 1), by low temperature training step by step, low temperature preserve and step by step activated at can effectively preserve worm's ovum.Under 14 hours every days illumination conditions, step by step alternating temperature process and low temperature preserve after ovum storage life be 17 days, now, compared with the incubation rate of the incubation rate of ovum and normal raising ovum at 25 ℃, without significant difference, and at 25 ℃, ovum can be preserved 3 days.When but after alternating temperature, low temperature is preserved 17 days suddenly, egg hatching rate is significantly lower than normal contrast of raising.Alternating temperature processing and low temperature were preserved after 22 days step by step, though the hatching of ovum has significant reduction compared with under normal raising, but it is still 75% under normal raising, and suddenly under alternating temperature, preserve after 22 days, the incubation rate of ovum is extremely low, be only 2% left and right under normal raising, two kinds of processing mode significant differences (referring to table 1).

After the method enforcement, show, the holding time of Eggs of The Rice Leaf Roller under illumination in 14 hours can extend to 22 days.

Incubation rate and the ratio that contrasts incubation rate at 25 ℃ after table 1 ovum preservation different number of days

Processing mode	Preservation number of days (my god)	Incubation rate and the ratio contrasting at 25 ℃
			CK	3	1.0a
Alternating temperature step by step	7	1.06±0.20a
				12	0.95±0.21ab
	17	0.89±0.20ab
				22	0.75±0.26b
	27	0.01±0.01c
				32	0.03±0.04c
Suddenly alternating temperature	17	0.76±0.10b
				22	0.02±0.02c
	24	0.0c

Note: alternating temperature preservation number of days is to preserve number of days at 15 ℃ to add cooling processing step by step 1 day (24 hours) and 1 day (24 hours) of cascade raising temperature processing step by step; It is remarkable that different lowercases represent to preserve between number of days incubation rate ratio difference.

Claims

1. one kind extends the method for Eggs of The Rice Leaf Roller storage life, it is characterized in that the Eggs of The Rice Leaf Roller of 25 ℃ of raisings to carry out successively after 23 ℃, 21 ℃, 19 ℃ and the 17 ℃ low temperature treatment step by step that keep respectively 2 hours, 4 hours, 8 hours and 10 hours, at 15 ℃ of low temperature, preserve 15 days, and then keep respectively the cascade raising temperature of 2 hours, 4 hours, 8 hours and 10 hours through 17 ℃, 19 ℃, 21 and 23 ℃ successively, at 25 ℃, hatch, extended ovum storage life.