CN112106734A

CN112106734A - Lugworm refrigeration diapause method

Info

Publication number: CN112106734A
Application number: CN202011042502.2A
Authority: CN
Inventors: 蔡翼杨; 谭燕; 肖慈平; 姜庭权; 郭子平; 杨颜
Original assignee: Qiandongnan Co of Guizhou Tobacco Co
Current assignee: Qiandongnan Co of Guizhou Tobacco Co
Priority date: 2020-09-28
Filing date: 2020-09-28
Publication date: 2020-12-22
Anticipated expiration: 2040-09-28
Also published as: CN112106734B

Abstract

The invention discloses a arma chinensis refrigeration diapause method, wherein the method comprises the following steps: (1) selecting Arcolinia chinensis armyworm eggs as cold-storage diapause objects; (2) and (2) placing the Armoracia chinensis armyworm eggs selected in the step (1) in a refrigeration diapause environment, wherein the refrigeration diapause temperature is 2-6 ℃, and the refrigeration diapause time is 8-12 days. Aiming at the excellent predatory natural enemy insect of the lugworm bugs, the invention develops control research for regulating and controlling the incubation time of lugworm bug armyworm eggs by using factors such as refrigeration temperature, refrigeration time and the like, and through research, the incubation rate of the lugworm bug armyworm eggs still reaches more than 50% after the lugworm bug armyworm eggs are kept still for 6-14 days in a refrigeration diapause environment with the temperature of 0-8 ℃, and the large-scale cultivation requirements can be basically met.

Description

Lugworm refrigeration diapause method

Technical Field

The invention relates to a lugworm refrigeration diapause method, and belongs to the technical field of lugworm cultivation.

Background

Arma chinensis is a natural enemy insect with a wide predation range, can predate more than forty common agricultural and forestry pests such as pine moth, inchworm, phylloxera, moth, scale insect, aphid, cabbage caterpillar and the like, and has a remarkable effect on controlling the population quantity of the pests. The application of the arma chinensis in biological control can greatly reduce the using amount of field and greenhouse pesticides, protect the ecological environment and ensure the safety of grains and foods.

The biological characteristics of arma chinensis are as follows: adults like crawling and are not good at flying, and move in the dark-shady part of the forest in summer to prey on various insects. When the temperature is 25-30 ℃ and the relative humidity is about 70%, the adult eclosion begins to copulate 7-14 days later. The life is finished about 24 times, and the maximum time is 31 times. Spawning begins 3-8 days after mating. Each female can lay 20 times or so in the first time, 20-30 eggs are laid in each time, the average egg laying amount is 300 eggs, and the maximum egg laying amount can reach 570 eggs. The oviposition period and the oviposition quantity are greatly influenced by the temperature, and the oviposition does not occur below 18 ℃ and above 40 ℃. The egg laying period can reach 100 days at 20-27 ℃, and the average egg laying amount is 465 grains. The average life of the adult (from overwintering) at 20-27 ℃ is 110 days; the service life is obviously shortened at high temperature, and the service life is only about 20 days at 35 ℃. The eggs are incubated for 7 days at the temperature of 25-30 ℃ and the relative humidity of 70 percent, and the hatchability is about 90 percent. Nymphs were 5 years old altogether. At 25 ℃ the nymph period is around 30 days, whereas at 30 ℃ it is only around 21 days. Nymphs at 1 st have a colonizing character and start to move dispersedly after 2 th. When the food is sufficient, the food is not like to spread, and when the food is not sufficient, the food can spread for about 10m at most after 7 days. Nymphs were essentially identical to adults.

At present, the applicant researches on the application of arma chinensis to the control of tobacco plant diseases and insect pests, but in the control, large number of arma chinensis needs to be cultivated. However, since the time from the spawn to the larva hatching of the lugworm armyworm ova is only 7 days, the propagation period is short, and the armyworm ova cannot be stored for a long time and applied in a timely manner, the lugworm ova becomes a technical bottleneck restricting the large-scale production and application of the lugworm.

Disclosure of Invention

Based on the above, the invention provides a arma chinensis refrigeration diapause method, which delays the development time of arma chinensis in a refrigeration mode and increases the propagation cycle of arma chinensis.

The technical scheme of the invention is as follows: an arma chinensis refrigeration diapause method, wherein the method comprises:

(1) selecting Arcolinia chinensis armyworm eggs as cold-storage diapause objects;

(2) and (2) placing the Armoracia chinensis armyworm eggs selected in the step (1) in a refrigeration diapause environment, wherein the refrigeration diapause temperature is 2-6 ℃, and the refrigeration diapause time is 8-12 days.

Optionally, the temperature of the cold storage diapause is 4 ℃, and the time of the cold storage diapause is 10 days.

Optionally, selecting armyworm eggs just produced by the armadilia chinensis in the step (1) as cold-stored diapause objects.

Optionally, the relative humidity of the refrigerated diapause is 60 ± 10%.

Optionally, the method further comprises a cold storage diapause pretreatment method, wherein the cold storage diapause pretreatment method comprises the following steps: before entering the refrigeration and diapause, the lugworm armyworm eggs are placed in an environment with the temperature of 10-15 ℃ for 1-2 days.

The invention has the beneficial effects that: aiming at the excellent predatory natural enemy insect of the lugworm bugs, the invention develops control research for regulating and controlling the incubation time of lugworm bug armyworm eggs by using factors such as refrigeration temperature, refrigeration time and the like, and through research, the incubation rate of the lugworm bug armyworm eggs still reaches more than 50% after the lugworm bug armyworm eggs are kept still for 6-14 days in a refrigeration diapause environment with the temperature of 0-8 ℃, and the large-scale cultivation requirements can be basically met.

Detailed Description

The invention relates to a arma chinensis refrigeration diapause method, which comprises the following steps:

(1) selecting Armoracia chinensis armyworm eggs as cold-storage diapause objects.

Preferably, the temperature for cold storage diapause is 4 ℃ and the time for cold storage diapause is 10 days.

Preferably, armyworm eggs laid by the lugworm bugs just now are selected as cold-stored diapause objects, and compared with armyworm eggs laid for a certain time, the cold-stored diapause effect of the armyworm eggs laid just now is better.

Preferably, the relative humidity of the refrigeration diapause is 60 +/-10%, the preservation temperature is suitable for Armoracia chinensis eggs, and the higher or lower temperature is not favorable for the refrigeration diapause of armyworm eggs.

Preferably, the method further comprises a cold storage diapause pretreatment method, wherein the cold storage diapause pretreatment method comprises the following steps: before entering the refrigeration and diapause, the lugworm armyworm eggs are placed in an environment with the temperature of 10-15 ℃ for 1-2 days. The method can improve hatching rate of the eggs of the armyworm.

Several experimental verifications were performed as described above:

test on temperature and time of cold storage and diapause

1. Cold storage diapause temperature

Taking armyworm eggs newly produced by Armoracia chinensis as test materials, dividing the armyworm eggs into 9 groups with 100 armyworm eggs per group, dividing the 9 groups into experimental groups 1 to 9, placing the experimental groups 1 to 9 in a refrigeration diapause environment (which can be an incubator) according to the temperature and time in the table 1, and setting the relative humidity of the refrigeration diapause to be 66%.

After the test is finished, each group of armyworm eggs are placed under the environment conditions of 26 ℃ and 66% of humidity, and the incubation time and the incubation amount of each group are counted.

TABLE 1

Experiment of	Temperature of	Time of day	Incubation time	Hatching amount	Hatching rate
						Experimental group 1	0℃	10 days	10 days	35	35％
Experimental group 2	1℃	10 days	10 days	43	43％
						Experimental group 3	2℃	10 days	9 days	45	45％
Experimental group 4	3℃	10 days	9 days	48	48％
						Experimental group 5	4℃	10 days	9 days	52	52％
Experimental group 6	5℃	10 days	9 days	50	50％
						Experimental group 7	6℃	10 days	9 days	46	46％
Experimental group 8	7℃	10 days	9 days	45	45％
						Experimental group 9	8℃	10 days	8 days	42	42％

The above tests show that when the refrigeration time is 10 days, the refrigeration diapause effect is the best at 4 ℃, which is obviously better than that of other experimental groups.

2. Cold storage and diapause time

Taking armyworm eggs newly produced by Armoracia chinensis as test materials, dividing the armyworm eggs into 9 groups with 100 armyworm eggs per group, dividing the 9 groups into experimental groups 1 to 9, placing the experimental groups 1 to 9 in a refrigeration diapause environment (which can be an incubator) according to the time and temperature in the table 2, and setting the relative humidity of the refrigeration diapause to be 66%.

TABLE 2

The above tests show that the incubation rate is higher the less the time is at 4 ℃ in the cold storage.

Second, the cold storage diapause pretreatment test verification

Taking armyworm eggs newly produced by Armoracia chinensis as test materials, dividing the armyworm eggs into 7 groups with 100 armyworm eggs in each group, taking the 6 groups as experiment groups 1 to 7, placing the armyworm eggs in each group in a refrigeration diapause environment (which can be an incubator) according to the temperature and time in the table 3 after the pretreatment method in the table 3, wherein the relative humidity of the refrigeration diapause is 66%.

After the test is finished, each group of armyworm eggs are placed under the environment conditions of 26 ℃ and 66% of humidity, and the hatching amount of each group is counted.

TABLE 3

Experiment of	Pretreatment	Temperature of	Time of day	Hatching amount	Hatching rate
						Experimental group 1	Without pretreatment	4℃	10 days	52	52％
Experimental group 2	10 ℃ for 1 day	4℃	10 days	56	56％
						Experimental group 3	10 ℃ for 2 days	4℃	10 days	55	55％
Experimental group 4	15 ℃ for 1 day	4℃	10 days	59	59％
						Experimental group 5	15 ℃ for 2 days	4℃	10 days	58	58％
Experimental group 6	20 ℃ for 1 day	4℃	10 days	54	54％
						Experimental group 7	20 ℃ for 2 days	4℃	10 days	53	53％

The above tests show that the hatchability can be improved by performing appropriate pretreatment before cold storage and diapause.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. An arma chinensis refrigeration diapause method, wherein the method comprises:

2. The armadilla bug cold storage diapause method of claim 2, wherein the temperature of cold storage diapause is 4 ℃ and the time of cold storage diapause is 10 days.

3. The armadilla chinensis refrigerated diapause method of claim 1, wherein armyworm eggs just produced by armadilla chinensis are selected as refrigerated diapause subjects in step (1).

4. The armadilla bug cold storage diapause method of claim 1, wherein the relative humidity of the cold storage diapause is 60 ± 10%.

5. The armadilla bug cold storage diapause method of claim 1, wherein the method further comprises a pre-cold diapause treatment method, wherein the pre-cold diapause treatment method is as follows: before entering the refrigeration and diapause, the lugworm armyworm eggs are placed in an environment with the temperature of 10-15 ℃ for 1-2 days.