CN110583587A - Armyworm rejuvenation method - Google Patents

Armyworm rejuvenation method Download PDF

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Publication number
CN110583587A
CN110583587A CN201911060915.0A CN201911060915A CN110583587A CN 110583587 A CN110583587 A CN 110583587A CN 201911060915 A CN201911060915 A CN 201911060915A CN 110583587 A CN110583587 A CN 110583587A
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China
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larvae
armyworm
corn leaves
providing
honey water
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CN201911060915.0A
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Chinese (zh)
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李鸿波
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GUIZHOU PLANT PROTECTION INSTITUTE
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GUIZHOU PLANT PROTECTION INSTITUTE
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The application provides a rejuvenation method of armyworms, comprising: corn leaves are used to feed mythimna larvae. By inoculating the armyworm back to the natural host corn leaves, the growth and development rate, the pupa weight and the egg laying amount of the armyworm are obviously improved, and further the method is expected to provide enough preys for the large-scale feeding of the arma chinensis.

Description

Armyworm rejuvenation method
Technical Field
The application relates to the field of arma bug propagation, in particular to a rejuvenation method for armyworm.
Background
The arma chinensis is a predatory natural enemy, can kill agricultural pests of various traditional Chinese medicines, and has great industrial production and application prospects. At present, the arma chinensis is mainly bred by utilizing armyworm, and the armyworm is mainly bred by artificial feed. However, the armyworm population shows degeneration phenomena of different degrees due to long-term utilization of artificial feed, and the degeneration phenomena are represented by prolonged development period, reduced pupa weight, reduced egg laying amount and the like. The cultivation efficiency is seriously influenced, the cultivation cost is increased, and even whether artificial propagation and industrialized production capacity are continuously developed is threatened, so that the arma chinensis becomes a bottleneck factor in the industrialized development of arma chinensis. Currently, insect rejuvenation methods are mainly performed by capturing wild species and crossing with indoor species. However, the armyworm is intermittent and seasonal, outdoor population cannot be obtained within a specific time, and the purification and rejuvenation effects are difficult to achieve.
Disclosure of Invention
In view of the above, the present application provides a rejuvenation method for armyworms. One or more of the problems due to the limitations and disadvantages of the related art are overcome at least in part.
The application provides a myxomatosis renaturation method, which comprises the following steps: corn leaves are used to feed mythimna larvae to rejuvenate the mythimna.
In some embodiments, armyworm rejuvenation includes at least one of:
growth and development rate, pupal weight and egg laying amount.
In some embodiments, said rearing of myxoplasma larvae with corn leaves to rejuvenate said myxoplasma comprises
And (3) putting the larvae of the myxoid into an insect breeding box to be bred by using corn leaves, wherein the ratio of the volume of the breeding box to the number of the larvae is 1L: 50 pieces.
The corn leaves are replaced regularly.
In some embodiments, further comprising:
and when the larva pupates, putting wet paper into the insect breeding box for pupation.
In some embodiments, the method further comprises:
when pupae emerge into adults, pairing the emerged adults;
inoculating the paired adults into an egg laying cage of about 700 ml;
providing a spawning medium, and providing honey water as a nutrient solution.
In some embodiments, the spawning medium is a plastic rope, the honey water has a mass fraction of 10%, and correspondingly, the providing the spawning medium and the honey water as the nutrient solution include:
providing a plastic rope as a spawning medium, and providing honey water with the mass fraction of 10% as a nutrient solution.
In some embodiments, the method further comprises:
replacing a spawning medium within a first preset time period;
and replacing the honey water within a second preset time.
The application has the advantages that:
according to the rejuvenation method for armyworms, the natural host corn leaves are inoculated in a reinjection mode, so that the growth and development rate, the pupa weight and the egg laying amount of the armyworms are remarkably improved, and further the method is expected to provide enough preys for large-scale feeding of the arma chinensis.
In addition to the objects, features and advantages described above, other objects, features and advantages will be apparent from the present application. The present application will be described in further detail below with reference to the drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the application and, together with the description, serve to explain the application and are not intended to limit the application.
Fig. 1 is a schematic flow chart illustrating a method for rejuvenating armyworms according to an embodiment of the present disclosure;
fig. 2 is a schematic flow chart illustrating a method for rejuvenating armyworms according to an embodiment of the present disclosure;
fig. 3 is a schematic flow chart illustrating a method for rejuvenating armyworms according to an embodiment of the present disclosure;
FIG. 4 is a schematic diagram of the comparison of the yield and longevity of armyworm of the tieback corn leaves and artificial feed provided in the examples of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
The application provides a rejuvenation method of armyworms, comprising: corn leaves are used to feed mythimna larvae to rejuvenate the mythimna.
In the embodiment of the present application, the armyworm is also called oriental armyworm, marching's worm, five-color caterpillar, and night bug. Belongs to Lepidoptera and Noctuidae, and is a migratory and fulminant pest. When the larvae bite the leaves of the plants to cause the plants to be incised into harmful plants, the leaves are usually eaten to form light stalks when the harmful plants are serious.
In an embodiment of the present application, the rejuvenation includes at least one of: growth and development rate, pupal weight and egg laying amount.
By inoculating the armyworm back to the natural host corn leaves, the growth and development rate, the pupa weight and the egg laying amount of the armyworm are obviously improved, and further the method is expected to provide enough preys for the large-scale feeding of the arma chinensis.
Fig. 1 is a schematic flow chart of a rejuvenation method for mythimna separata provided in the present application, and as shown in fig. 1, the method includes:
step S101, putting the larvae of the myxoplasma into an insect breeding box for breeding by using corn leaves, wherein the ratio of the volume of the breeding box to the number of the larvae is 1L: 50 pieces.
And step S102, periodically replacing the corn leaves.
By inoculating the armyworm back to the natural host corn leaves, the growth and development rate, the pupa weight and the egg laying amount of the armyworm are obviously improved, and further the method is expected to provide enough preys for the large-scale feeding of the arma chinensis.
Fig. 2 is a schematic flow chart illustrating an implementation of the rejuvenation method for mythimna separata according to an embodiment of the present application, and as shown in fig. 2, the method includes:
step S201, putting the larvae of the myxoplasma into an insect breeding box for breeding by using corn leaves, wherein the ratio of the volume of the breeding box to the number of the larvae is 1L: 50 pieces.
Step S202, periodically replacing the corn leaves.
And S203, when the larva pupates, putting wet paper into the insect breeding box for pupation.
According to the method provided by the embodiment of the application, the corn leaves are used for feeding the armyworms, and the wet paper is put into the armyworms for pupating, so that the pupa weight can be increased.
An embodiment of the present application provides a method for rejuvenating armyworms, and fig. 3 is a schematic flow chart of an implementation of the method for rejuvenating armyworms provided in the embodiment of the present application, as shown in fig. 3, the method includes:
step S301, putting the larvae of the myxoplasma into an insect breeding box for breeding by using corn leaves, wherein the ratio of the volume of the breeding box to the number of the larvae is 1L: 50 pieces.
And step S302, periodically replacing the corn leaves.
Step S303, when the larva pupates, moist paper is put into the larva breeding box for pupation.
And S304, when the pupae emerge into imagoes, pairing the emerging imagoes.
And S305, inoculating the paired adults into an egg laying cage of about 700 ml.
Step S306, providing a spawning medium and providing honey water as a nutrient solution.
In the embodiment of the application, the oviposition medium can be a plastic rope, and the mass percentage of the honey water is 10%.
In the embodiment of the application, the larva is fed through the pupa corn leaves, the pupa eclosion is carried out to form the imago, the imago is paired and is placed into the 700mL oviposition cage, oviposition media and honey water are provided, and the oviposition amount of the imago can be improved.
In some embodiments, the method further comprises:
and replacing the oviposition medium within a first preset time.
In this embodiment of the application, the first preset time period may be 2 days, or may be 3 days.
And replacing the honey water within a second preset time.
In this embodiment of the application, the second preset time period may be 1 day, or may be 2 days.
Experimental example:
the experimental method comprises the following steps:
egg masses laid by indoor degeneration populations are collected, placed in a 1050 ml cylindrical box, and placed in a wet cotton ball for moisturizing, and the hatching condition of the eggs is observed every day. After the egg mass is hatched, 100 larvae of 1 year old are placed into a box containing fresh corn leaf insect-breeding boxes for breeding (the volume is 2L), corn leaves are replaced periodically, and the survival condition of armyworm larvae is observed every day. After the larvae are mature, putting wet tissue paper into the larva breeding box for pupation. And counting pupation rate after pupation, and counting pupation weight of each treatment armyworm. And counting the eclosion rate after the pupae eclosion becomes imagoes. Armyworm fed directly was used as a control. Repeat 4 times.
Comparison of reproduction and Life
Selecting the eclosion-treated adults for pairing, inoculating the eclosion-treated adults into an egg laying cage of about 700ml, providing a plastic rope as an egg laying medium, and providing 10% honey water as nutrition. And observing the oviposition condition of the armyworms every day from the third day, taking out the armyworms after the oviposition condition of the armyworms is found to exist on the plastic ropes, and providing new plastic ropes for the armyworms to continuously oviposit eggs. The honey water needs to be changed every day to ensure that the quality is not deteriorated until the adult is dead. Finally, the service life and the egg laying amount of the adults under different treatments are counted, and each treatment is repeated for 20 times.
A comparison of growth development was first made, table 1 influence of artificial feed and tieback corn leaves on armyworm growth development, pupal weight and survival rate. As shown in Table 1, the larval stage of armyworm after the host corn leaves are inoculated back is shortened by 3.23 days compared with the larval stage of the armyworm fed by artificial feed, but the larval stage of the armyworm and the larval stage of the armyworm have no significant influence on the larval stage of the armyworm. The inoculation of the host had a significant effect on the weight of armyworm pupae. For example, the pupae weight of armyworm raised with artificial feed was 323.56 mg, which is significantly lower than that of the back-inoculated host-raised group (369 mg). In terms of survival, the survival rates of the larval stage and the pupal stage of the group fed with artificial feed were 81.25% and 90.77%, respectively, which were lower than those of the back-inoculated host group (86.25% and 91.30%, respectively). The total survival rate of the artificial feed breeding is 73.75 percent, and the total survival rate of the artificial feed breeding inoculated with the host is 78.75 percent.
And then, reproduction and life comparison are carried out, fig. 4 is a schematic diagram for comparing the yield and life of the armyworm by the tieback corn leaves and the artificial feed provided in the embodiment of the application, as shown in fig. 4, the analysis of the egg laying amount of the tieback corn leaves and the artificial feed shows that the egg laying amount of each female of the adult feed composition insects is 532.73 eggs, and the tieback corn leaf feeding group is 770.88 eggs, which are obviously different. The service lives of the artificial feed group and the corn leaf adult are not obviously different, and in the corn feeding group, the service life of male worms is obviously longer than that of female worms; within the maize leaf feeding group, there was no significant difference in the life span of males and females.
By grafting back natural host corn leaves, the growth and development rate, the pupa weight and the egg laying amount of armyworms are obviously improved. Therefore, the inoculation of the natural host corn leaves can have the effect of purifying and rejuvenating the armyworm population.
In the description herein, reference to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.
The above description is only for the specific embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily conceive various changes or substitutions within the technical scope of the present application, and these should be covered by the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

Claims (7)

1. A rejuvenation method for armyworms, comprising:
corn leaves are used to feed mythimna larvae to rejuvenate the mythimna.
2. The method of claim 1, wherein rejuvenation comprises at least one of:
growth and development rate, pupal weight and egg laying amount.
3. The method of claim 1 or 2, wherein said rearing larvae of myxoplasma with corn leaves to rejuvenate said myxoplasma comprises:
and (3) putting the larvae of the myxoid into an insect breeding box to be bred by using corn leaves, wherein the ratio of the volume of the breeding box to the number of the larvae is 1L: 50 pieces of the Chinese herbal medicine are added;
the corn leaves are replaced regularly.
4. The method of claim 3, further comprising:
when the larva pupates, moist paper is put into the insect breeding box for pupation.
5. The method of claim 4, further comprising:
when pupae emerge into adults, pairing the emerged adults;
inoculating the paired adults into an egg laying cage of about 700 ml;
providing a spawning medium, and providing honey water as a nutrient solution.
6. The method according to claim 5, wherein the spawning medium is a plastic rope, the honey water is 10% by mass, and correspondingly, the providing the spawning medium and the honey water as a nutrient solution comprises:
providing a plastic rope as a spawning medium, and providing honey water with the mass fraction of 10% as a nutrient solution.
7. The method of claim 6, further comprising:
replacing a spawning medium within a first preset time period;
and replacing the honey water within a second preset time.
CN201911060915.0A 2019-11-01 2019-11-01 Armyworm rejuvenation method Pending CN110583587A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115380876A (en) * 2022-10-09 2022-11-25 北京蓝狐天敌技术有限公司 Armoracia chinensis population rejuvenation method

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JPS58189101A (en) * 1983-03-28 1983-11-04 Otsuka Pharmaceut Co Ltd Communication disturber against male adults of harmful insects
US20120034288A1 (en) * 2010-08-04 2012-02-09 Mckibben Gerald H Compositions and methods for enhanced attraction of noctuid moths
CN103004704A (en) * 2013-01-05 2013-04-03 北京市西山试验林场 Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts
CN105475239A (en) * 2015-12-18 2016-04-13 中国农业科学院草原研究所 Exorista civilis rondani rejuvenation method
CN106172251A (en) * 2016-07-27 2016-12-07 中国农业科学院植物保护研究所 A kind of method for breeding of Mythimna Lorgi
CN107432371A (en) * 2017-09-30 2017-12-05 贵州省烟草公司遵义市公司绥阳县分公司 A kind of man-made feeds of suitable mythimna separata large-scale breeding and preparation method thereof
CN107581160A (en) * 2017-09-30 2018-01-16 贵州省烟草公司遵义市公司绥阳县分公司 A kind of artificial scale breeding method of mythimna separata
CN109169531A (en) * 2018-10-11 2019-01-11 吉林农业大学 Armyworm trichogramma domestication mating system and control and application suitable for eating-core bean worm prevention and treatment

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58189101A (en) * 1983-03-28 1983-11-04 Otsuka Pharmaceut Co Ltd Communication disturber against male adults of harmful insects
US20120034288A1 (en) * 2010-08-04 2012-02-09 Mckibben Gerald H Compositions and methods for enhanced attraction of noctuid moths
CN103004704A (en) * 2013-01-05 2013-04-03 北京市西山试验林场 Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts
CN105475239A (en) * 2015-12-18 2016-04-13 中国农业科学院草原研究所 Exorista civilis rondani rejuvenation method
CN106172251A (en) * 2016-07-27 2016-12-07 中国农业科学院植物保护研究所 A kind of method for breeding of Mythimna Lorgi
CN107432371A (en) * 2017-09-30 2017-12-05 贵州省烟草公司遵义市公司绥阳县分公司 A kind of man-made feeds of suitable mythimna separata large-scale breeding and preparation method thereof
CN107581160A (en) * 2017-09-30 2018-01-16 贵州省烟草公司遵义市公司绥阳县分公司 A kind of artificial scale breeding method of mythimna separata
CN109169531A (en) * 2018-10-11 2019-01-11 吉林农业大学 Armyworm trichogramma domestication mating system and control and application suitable for eating-core bean worm prevention and treatment

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115380876A (en) * 2022-10-09 2022-11-25 北京蓝狐天敌技术有限公司 Armoracia chinensis population rejuvenation method

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