CN106172251A - A kind of method for breeding of Mythimna Lorgi - Google Patents
A kind of method for breeding of Mythimna Lorgi Download PDFInfo
- Publication number
- CN106172251A CN106172251A CN201610603046.1A CN201610603046A CN106172251A CN 106172251 A CN106172251 A CN 106172251A CN 201610603046 A CN201610603046 A CN 201610603046A CN 106172251 A CN106172251 A CN 106172251A
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- China
- Prior art keywords
- breeding
- mythimna
- lorgi
- adult
- larva
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 241001477928 Mythimna Species 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000009395 breeding Methods 0.000 title claims abstract description 17
- 230000001488 breeding effect Effects 0.000 title claims abstract description 17
- 239000002689 soil Substances 0.000 claims abstract description 16
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 12
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 12
- 240000008042 Zea mays Species 0.000 claims abstract description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 12
- 235000005822 corn Nutrition 0.000 claims abstract description 12
- 210000004681 ovum Anatomy 0.000 claims abstract description 12
- 230000012447 hatching Effects 0.000 claims abstract description 10
- 241000382353 Pupa Species 0.000 claims abstract description 9
- 230000003203 everyday effect Effects 0.000 claims abstract description 8
- 230000008859 change Effects 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 235000013601 eggs Nutrition 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 7
- 230000003020 moisturizing effect Effects 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 5
- 230000000384 rearing effect Effects 0.000 claims description 5
- 238000007906 compression Methods 0.000 claims description 2
- 210000003608 fece Anatomy 0.000 claims description 2
- 230000036541 health Effects 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 210000000582 semen Anatomy 0.000 abstract description 9
- 230000017448 oviposition Effects 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 5
- 239000013589 supplement Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract description 2
- 241000409991 Mythimna separata Species 0.000 description 10
- 241000238631 Hexapoda Species 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000618568 Mythimna loreyi Species 0.000 description 1
- 241000256259 Noctuidae Species 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000007230 Sorghum bicolor Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to the method for breeding of a kind of Mythimna Lorgi.The method mainly includes following step: the preservation of (1) ovum and hatching: ovum can be hatched under the conditions of 4 DEG C as required；(2) 13 instar larvaes: the larva of hatching needs to add new fresh and tender little leaf of Semen Maydis；(3) 46 instar larvaes: 3 latter stage in age larva start subpackage, 10/750ml vial, change fresh corn leaf every day；(4) pupa: mature larva pupates to need in the sterile soil of aqueous 10%；(5) adult is collected: the adult of emergence needs to supplement the nutrients.Use the breeding technology of a kind of Mythimna Lorgi of the present invention, temperature 24 DEG C, photophase: under the conditions of dark phase=14:10h, humidity 70%, there is survival rate height, egg laying amount height, raise the advantages such as simple and convenient, can be used for scientific research and raise on a large scale.
Description
Technical field
The present invention relates to a kind of insecticide breeding technology, be specifically related to the raising side of a kind of Agriculture Products Mythimna Lorgi
Method.
Background technology
Mythimna Lorgi Leucania loreyi belongs to Lepidoptera Noctuidae, is a kind of worldwide insect, Africa, Asia, in
East and Australia have occurred, and many generations, desirable drinking water rice, Semen Tritici aestivi, Fructus Hordei Vulgaris, Sorghum vulgare Pers., Semen Maydis and Caulis Sacchari sinensis can occur every year.
In China's main harm Semen Tritici aestivi, Semen Maydis and Oryza sativa L., there is presently no with primary pest report, in south China area usually with east
Side's mythimna separata Mythimna separata mixing occurs.Oriental armyworm is a kind of main cereal crops insect of China, has typical case
Habit of migrating, in China north and south come and go migrate.The form of Mythimna Lorgi larva and pupa and oriental armyworm be quite similar be difficult to by
Distinguishing, and their development duration is different, the development duration of oriental armyworm each worm state is all than Lloyd's at different temperatures
Mythimna separata is fast.These cause interference all to the prediction of oriental armyworm, grass-roots work personnel knowledge incorrect to Mythimna Lorgi
Not also possible is that it is become primary pest from minor pests.
Because not enough to the understanding of Mythimna Lorgi larva and pupa, bring the biggest puzzlement to observing and predicting work.Therefore, add
The strong scientific research to Mythimna Lorgi, especially its biological habit aspect.In laboratory, carry out research must first have to build
Erecting laboratory population, this most just requires that its method for breeding is had certain understanding.But, due to Mythimna Lorgi at present or one
Planting minor pests, its research is not the most caused enough attention, method for breeding does not has relevant report yet.
Summary of the invention
It is an object of the invention to provide a kind of Mythimna Lorgi laboratory rearing method, have that survival rate is high, egg laying amount is high,
Raise the advantages such as simple and convenient, can be used for scientific research and raise on a large scale.
A kind of method for breeding of Mythimna Lorgi, including order below step:
1) egg hatching is to 1-3 instar larvae: ovum uses fresh green corn leaf to protect in vial under the conditions of 18-30 DEG C
Wet, and change hatching every day；Add fresh green corn during worm's ovum blackening, wait for hatching quietly to 1-3 instar larvae；
2) 4-5 instar larvae: by larva subpackage to vial, every day changes fresh green corn leaf, and cleans vial
The feces of interior insect；
3) pupa: the mature larva that compression process health of experience being hung shortens is chosen into water content in the sterile soil of 8-10%, and
Add appropriate fresh green corn leaf；
4) adult: catch out by the adult of emergence, male and female pairing is put in glass enclosure, has absorption in described bell glass
The cotton mass having concentration to be 10% hydromel；Put into paper folding two days later, lay eggs for adult.
Described step (2) is packed as 10 larvas/750ml vial.
The preparation method of described sterile soil: by soil oven for drying 24h of 100 DEG C, add appropriate water and stir
To water content at 8-10%.
Described step 3) in sterile soil add 750ml bottle bottom, soil thickness is at 10-12cm, and every bottle adds 10
Pupa.
Described step 4) in cotton mass be placed in surface plate, and every day change.
Described bell glass size is 1500ml.
Described ovum preserves under the conditions of 4 DEG C.
Described step 1), 2), 3), 4) rearing conditions: temperature 24 DEG C, photophase: dark phase=14:10h, humidity 70%.
Owing to Mythimna Lorgi and oriental armyworm are sibling specieses, therefore the method for breeding of this experimental evidence oriental armyworm (Jiang Shanjun,
Luo Li intelligence .2010) improve and explore.First, specify that preservation condition and the time of ovum, can select to incubate the most in good time
Changing, the moisturizing to ovum uses fresh corn leaf nature moisturizing.Find that picking newly hatched larvae easily causes the dead of larva through overtesting
Die, and the Mythimna Lorgi larval feeding of front 3 ages is less.Therefore, before selecting not remove subpackage on the premise of moisturizing, 3 ages are young
Worm.Required soil layer of pupating is thickeied, and soil has carried out high temperature sterilize, the most both ensure that pupate required wet
Degree prevents again soil microorganism to infect pupa.Needed for Adult supplement nutrient, the concentration of hydromel is higher than oriental armyworm, Mel
The concentration level of water directly affects the egg laying amount of Mythimna Lorgi.
A kind of Mythimna Lorgi laboratory rearing method of the present invention, has that survival rate is high, egg laying amount high, it is simple and convenient to raise
Etc. advantage, can be used for scientific research and raise on a large scale.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment (rearing conditions: temperature 24 DEG C, photophase: dark phase=14:10h, humidity 70%, and be all constant)
1) hatch: take out and be saved in the Mythimna Lorgi ovum under the conditions of 4 DEG C and put in vial, add enough new in bottle
Fresh and tender Corn leaf moisturizing, changes a leaf of Semen Maydis in two days, finds soon to hatch during worm's ovum blackening, now cannot the most more
Change leaf of Semen Maydis, prevent from losing the larva of hatching, can only be the most again with leaf of Semen Maydis.
2) 1-3 instar larvae: the larva in this period does not takes food, if the leaf of Semen Maydis ensured in bottle be fresh i.e.
Can, multiple moisturizing can be carried out to adding a cover wet towel on bottle.The larva tried not this period of subpackage, it is to avoid artificial
Mechanical damage.The age of larva is generally 2-3 days, and often one age of increase all can once cast off a skin.
3) 4-6 instar larvae: the larva of 3 age Mos carries out subpackage, with little brush pen gently choose in vial, 10/bottle.This
The larva in individual period has entered into the phase of taking food, and 5-6 is the gluttony phase of Mythimna Lorgi age, removes excrement except changing feedstuff every morning
Just outside, also needing to evening observe once, should supplement in time if lacking food, because the food ingestion of insect in evening is than daytime more more
Greatly.
4) in pupa time: be first ready for sterilizing, by oven for drying 24h of 100 DEG C, add appropriate water and stir guarantor
Card water content is about 10%.The soil being stirred is joined vial and has 10cm soil layer.Finally by the aging of contracting of hanging
Larva is chosen in bottle inlet, and it is a little that 10/bottle add fresh corn leaf.
5) adult: catch out by the adult of emergence, male and female pairing is put in 1500ml glass enclosure, places into and joins before
The hydromel of good concentration 10%, hydromel is contained in be placed with in the little culture dish of cotton mass and can not have open fire, and every day enters
Row supplements and changes.Need two days later to put into the paper folding that folding fan is the same, lay eggs for adult.Collect ovum bar after laying eggs to protect
Deposit.
Five, result of the test
The development duration of Mythimna Lorgi each worm state, survival rate and female worm reproductive capacity under the different raising temperature of table 1
In table, data are average ± standard error, multiple with representing Tukey ' HSD containing same letter after data line
Comparing difference is not notable (P < 0.05)
Mythimna Lorgi life table parameters under the different raising temperature of table 2
In table, data are average ± standard error, multiple with representing Tukey ' HSD containing same letter after data line
Comparing difference is not notable (P < 0.05)
Mythimna Lorgi 3 generation growing state at 3 24 DEG C of table
In table, data are average ± standard error, multiple with representing Tukey ' HSD containing same letter after data line
Comparing difference is not notable (P < 0.05)
Result of the test shows, at 24 DEG C, Mythimna Lorgi net added value rate, reproductive capacity are significantly higher than other temperature, have again higher
Intrinsic rate of increase and finite rate of increase, mean time is the shortest for the cycle, is the temperature being best suitable for Mythimna Lorgi indoor growing.24
The test data raising Mythimna Lorgi three generations at DEG C shows, Mythimna Lorgi mythimna separata for many generations can be raised in indoor.The first generation by
May adapt to indoor environment in it, survival rate of larvae, egg laying amount, oviposition duration, Life of Adult etc. are all significantly less than the second filial generation
With the third generation, the second filial generation and third generation indices all tend towards stability without significant difference.Result above shows, temperature 24 DEG C,
Under conditions of humidity 70% ± 5, photoperiod 14L:10D, the indoor feeding of Mythimna Lorgi can be carried out according to step 1-5.
Claims (8)
1. a method for breeding for Mythimna Lorgi, including order below step:
1) egg hatching is to 1-3 instar larvae: ovum uses fresh green corn leaf moisturizing in vial under the conditions of 18-30 DEG C, and
Every day changes hatching；Add fresh green corn during worm's ovum blackening, wait for hatching quietly to 1-3 instar larvae；
2) 4-5 instar larvae: by larva subpackage to vial, every day changes fresh green corn leaf, and cleans worm in vial
The feces of son；
3) pupa: the mature larva that compression process health of experience being hung shortens is chosen into water content in the sterile soil of 8-10%, and adds
Appropriate fresh green corn leaf；
4) adult: catch out by the adult of emergence, male and female pairing is put in glass enclosure, is adsorbed with dense in described bell glass
Degree is the cotton mass of 10% hydromel；Put into paper folding two days later, lay eggs for adult.
Method for breeding the most according to claim 1, is packed as 10 larvas/750ml vial in described step (2).
Method for breeding the most according to claim 1, the preparation method of described sterile soil: soil is dried at the baking oven of 100 DEG C
Dry 24h, adds appropriate water and is evenly stirred until water content at 8-10%.
Method for breeding the most according to claim 1, described step 3) in sterile soil add 750ml bottle bottom, soil layer is thick
Spending at 10-12cm, every bottle adds 10 pupas.
Method for breeding the most according to claim 1, described step 4) in cotton mass be placed in surface plate, and every day is more
Change.
Method for breeding the most according to claim 1, described bell glass size is 1500ml.
Method for breeding the most according to claim 1, preserves before described egg hatching under the conditions of 4 DEG C.
Method for breeding the most according to claim 1, described step 1), 2), 3), 4) rearing conditions: temperature 24 DEG C, light
Phase: dark phase=14:10h, humidity 70%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610603046.1A CN106172251A (en) | 2016-07-27 | 2016-07-27 | A kind of method for breeding of Mythimna Lorgi |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610603046.1A CN106172251A (en) | 2016-07-27 | 2016-07-27 | A kind of method for breeding of Mythimna Lorgi |
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| CN106172251A true CN106172251A (en) | 2016-12-07 |
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| CN201610603046.1A Pending CN106172251A (en) | 2016-07-27 | 2016-07-27 | A kind of method for breeding of Mythimna Lorgi |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108112544A (en) * | 2018-01-30 | 2018-06-05 | 中国农业科学院草原研究所 | Accurate method for breeding in a kind of age-group phase room of armyworm larvae |
| CN110583587A (en) * | 2019-11-01 | 2019-12-20 | 贵州省植物保护研究所 | Armyworm rejuvenation method |
| CN112493211A (en) * | 2020-11-16 | 2021-03-16 | 河南省农业科学院植物保护研究所 | Clavicia laurensis colony breeding device and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007111003A (en) * | 2005-10-21 | 2007-05-10 | Matsushita Electric Works Ltd | Method and system for controlling insect pest |
| CN102578432A (en) * | 2012-03-27 | 2012-07-18 | 董志平 | Artificial feed preparation and simplified feeding method for Athelis lepigone larva |
| FR3007940A1 (en) * | 2013-07-05 | 2015-01-09 | Mesange Verte | DEVICE FOR COLLECTING AND DESTRUCTING PINE PROCESSIONAL TRACKS |
| CN104872387A (en) * | 2015-05-26 | 2015-09-02 | 桐城市杨清土元养殖有限公司 | Sculellaria barbata containing ground beeltle larva fodder |
-
2016
- 2016-07-27 CN CN201610603046.1A patent/CN106172251A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007111003A (en) * | 2005-10-21 | 2007-05-10 | Matsushita Electric Works Ltd | Method and system for controlling insect pest |
| CN102578432A (en) * | 2012-03-27 | 2012-07-18 | 董志平 | Artificial feed preparation and simplified feeding method for Athelis lepigone larva |
| FR3007940A1 (en) * | 2013-07-05 | 2015-01-09 | Mesange Verte | DEVICE FOR COLLECTING AND DESTRUCTING PINE PROCESSIONAL TRACKS |
| CN104872387A (en) * | 2015-05-26 | 2015-09-02 | 桐城市杨清土元养殖有限公司 | Sculellaria barbata containing ground beeltle larva fodder |
Non-Patent Citations (3)
| Title |
|---|
| 张蕾等: "一日龄饥饿对粘虫成虫卵巢发育和飞行能力的影响", 《昆虫学报》 * |
| 江幸福等: "饲养温度对粘虫飞行和生殖能力的影响", 《生态学报》 * |
| 路子云等: "粘虫及其寄生性天敌―管侧沟茧蜂饲养方法", 《环境昆虫学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108112544A (en) * | 2018-01-30 | 2018-06-05 | 中国农业科学院草原研究所 | Accurate method for breeding in a kind of age-group phase room of armyworm larvae |
| CN110583587A (en) * | 2019-11-01 | 2019-12-20 | 贵州省植物保护研究所 | Armyworm rejuvenation method |
| CN112493211A (en) * | 2020-11-16 | 2021-03-16 | 河南省农业科学院植物保护研究所 | Clavicia laurensis colony breeding device and method |
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Application publication date: 20161207 |
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