KR20190051322A - Method for artificial incubation of Zophobas atratus Fabricius - Google Patents

Abstract

The present invention relates to a method for artificial hatching of Zophobas morio. The method for artificial hatching of Zophobas morio according to the present invention comprises conditions of: (a) a hatching temperature of 29-30°C; (b) long-day conditions of 12L : 12D to 15L : 9D; and (c) illuminance of more than 600 to 1,800 lux or less. According to the present invention, as the optimum hatching condition of Zophobas morio is identified, mass-production thereof becomes possible. The present invention also allows stable production of a plan, enabling activation of the insect industry and bringing an increase in income while being used in a farmhouse efficiently, and thus the labor efficiency can be expected to increase.

Description

Method for Artificial Incubation of Zophobas atratus Fabricius

More particularly, the present invention relates to a method for artificially hatching an American king's goat by controlling a specific temperature, light period, and illuminance in order to increase the hatching rate in a short time of the American king's goat .

Insects are one of the important roles in natural ecosystems because they are used as food for animals because many insect species are nourishing abundantly. Domestic animals have been used as feed for pets for more than 10 years. They have begun breeding in insect lovers and zoos. Recently, as a feed insect, there are many farmers who are proliferating for industrialization.

Especially, the most commonly used animal feed insects are Tenebrionidae insects. Several studies have been conducted on the growth characteristics, artificial breeding methods and artificial feeds of these goats and insects.

Related prior arts include Korean Patent No. 10-1660379 (a method for shortening the development period of brown larvae), Korean Patent Laid-Open No. 10-2017-0052128 (feed composition for rearing of larvae and larvae), Korean Patent No. 10-1508742 (A jelly-like composition for supplying water to the goats and larvae and a method for producing the same) and the like are known.

Although many studies on goose and insects have been carried out, most have been mentioned about the developmental characteristics of larvae or adults of brown ducks, and some of the normal hatching temperatures for American goats have been partially known, The optimal hatching condition for increasing the hatching rate has not yet been studied accurately.

It is an object of the present invention to provide a method for artificial hatching of an American king's goat which can be mass-produced while easily exploiting the optimal hatching condition of the American king's goat.

(B) a long-day condition 12L: 12D to 15L: 9D; (C) a degree of roughness exceeding 600 or more; More preferably, the egg of the American kingpin is hatched at a hatched temperature of 30 DEG C, hatched under long-day conditions of 12L: 12D, or hatched at 1,800 Lux Lt; / RTI >

When the above-mentioned hatching condition is applied, the egg of the American king duck shows a hatching rate of 80 to 90% for 5 to 7 days, and it shows a high hatching rate in a short period of time.

The technical objects to be achieved by the present invention are not limited to the above-mentioned technical problems.

According to the present invention, as the optimal hatching condition of the American king crab is clarified, it is possible to mass-produce it, and it is possible to stably plan production, thereby enabling activation of the insect industry and increase in income, and at the same time, , It is expected to increase labor efficiency.

FIG. 1 is a graph showing hatching hatchlings of American kingfishers by incubation temperature.
FIG. 2 is a graph showing the daily hatching rate of the American kingpin depending on the incubation temperature.
FIG. 3 is a graph showing the hatching rate of the American kingpins by the light weeks.
FIG. 4 is a graph showing the daily hatching rate of the American kingpin according to the photoperiod.
FIG. 5 is a graph showing hatching rate of the American kingpins by illuminance.
FIG. 6 is a graph showing daily hatching rates of the American Royal Ducks according to the illuminance.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail, and a detailed description of known functions and configurations that may unnecessarily obscure the gist of the present invention will be omitted.

(A) a hatching temperature of 29 ° C to 30 ° C, (b) a long-day condition of 12L, and a long-term hatching condition of 12-25 liters per day. In order to achieve the above object, : 12D to 15L: 9D, (C) Roughness of more than 600 to less than 1,800. In the environment of Lux, it was confirmed that the American hatchling egg showed a high hatching rate in a short time.

Hereinafter, a method for artificially hatching the American King's gourd according to the present invention will be described in detail.

All insects are significantly affected by development, hatching, and other conditions depending on the conditions such as temperature, light period, and illumination applied during artificial breeding.

Thus, in the present invention, the hatching temperature at hatching temperature of 29 ° C to 30 ° C at the time of hatching of the eggs of the American king crab, (b) long day conditions 12L: 12D to 15L: 9D, (C) As a specific example, it has been confirmed that the egg of the American King's egg hatch has a high hatching rate in a short period of time at a hatching temperature of 30 ° C or a long-day condition of 12L: 12D, or under a condition of 1,800 Lux, When the above conditions are exceeded, the hatching rate is rather low, and it is confirmed that the hatching condition is an essential condition to be applied when hatching eggs of the American king.

The hatching period and the hatching rate of the American hatchlings hatching showed that the hatching rate was 80 to 90% for 5 to 7 days. The hatching rate was high in a short time. Recently, Considering the point of view, it is possible to supply the material smoothly according to the increase in demand, and it can be easily used at the farmhouse, and the labor efficiency can be expected to increase.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Example 1 Identification of Temperature Characteristics Suitable for Improving the Hatching Rate of the American King Goose

1. Experimental Method

1) Experimental insects and numbers: 13G eggs (eggs incubated at 30 ℃, 65% R.H for 1 day), 100 eggs per test day, 2 repeats

2) Experiment temperature: 25 ℃, 27 ℃, 30 ℃, 33 ℃

3) Survey method and period: once a day, 10 days from the beginning

4) Breeding conditions: humidity: 65 ± 5%, light intensity: 1800lux, light period: 9L / 15 (general insect rearing condition)

5) Statistical analysis: Chi-square test, X ² = 51.417, df = 3, p = 0.0001

                    Oneway ANOVA test, F (3,8) = 8.020, p = 0.009

2. Experimental results

The results of the experiment are shown in Figs.

<The number of daily hatchings of the American Royal Ducks by temperature> Temperature
(° C) Hatching days (days) Total volume
(Mari) 4 days 5 days 6 days 7 days 8 days 9th 10 days 25 - - - 13 88 20 8 129/200 27 - - 26 116 20 - - 162/200 30 - 54 119 9 - - - 182/200 33 8 115 9 One - - - 133/200

<The daily hatching rate of the American Royal Ducks according to temperature> Temperature
(° C) Hatching days (days) Total hatching rate
(%) 4 days 5 days 6 days 7 days 8 days 9th 10 days 25 - - - 6.5 44.0 10.0 4.0 64.5 27 - - 13.0 58.0 10.0 - - 81.0 30 - 27.0 59.5 4.5 - - - 91.0 33 4.0 57.5 4.5 0.5 - - - 66.5

As shown in FIGS. 1 and 2 and Tables 1 and 2, when the hatching rate of the American king crab at four temperatures including 25 ° C., 27 ° C., 30 ° C. and 33 ° C. was examined, 30 ° C. was the highest at 91.0% It was confirmed that the hatching rate was significantly influenced by temperature in the order of 27 ℃ 81.0%, 33 ℃ 66.5% and 25 ℃ 64.5%.

In addition, the daily hatching rate by temperature was investigated. As the temperature decreased, the number of hatching days tended to be longer. That is, incubation was carried out at 25 ° C for 7-10 days, at 27 ° C for 6-8 days, at 30 ° C for 5-7 days, and at 33 ° C for 4-7 days, and at 30 ° C, the hatching rate was 86.5% between 5-6 days Respectively. In conclusion, in order to hatch the hatchlings of the Americans at once and raise the hatching rate, it is appropriate to store the American gill kernels at 29 to 30 ° C, and more preferably to store at 30 ° C.

Example 2: Identification of photoperiod characteristics suitable for improving the hatching rate of the American king crab

1. Experimental Method

1) Experimental insects and numbers: 14G eggs (eggs incubated at 30 ℃, 65% R.H for 1 day), 100 eggs per test day, 3 repeats

2) Experimental photoperiod: 9L / 15D, 12L / 12D, 15L / 9D

3) Survey method and period: once a day, 7 days from the beginning

4) Breeding conditions: temperature: 30 ° C, humidity: 65 ± 5%, illuminance: 1,800lux

5) Statistical analysis: Chi-square test, X ² (2) = 1.655, p = 0.437

                    Oneway ANOVA test, F (2,6) = 0.535, p = 0.611

2. Experimental results

The results of the experiment are shown in FIGS.

<The number of daily hatchings of the American King Gojari according to Gwangju period> Guangzhou Hatching days (days) Total volume
(Mari) 4 days 5 days 6 days 7 days 9L / 15D 3 171 63 - 237/300 12L / 12D 20 210 22 - 252/300 15L / 9D - 135 110 - 245/300

<The Daily Hatching Rate of the American King Gojari According to Gwangju Period> Guangzhou Hatching days (days) Total hatching rate
(%) 4 days 5 days 6 days 7 days 9L / 15D 1.0 57.0 21.0 - 79.0 12L / 12D 6.7 70.0 7.3 - 84.0 15L / 9D - 45.0 36.7 - 81.7

As shown in FIGS. 3 and 4 and in Tables 3 and 4, when the temperature was fixed at 30 ° C. and hatching rates of the American kingpins were examined for three light periods such as 9L / 15D, 12L / 12D and 15L / 9D, 12D was the highest at 84.0%, followed by 15L / 9D, 81.7%, 9L / 15D and 79.0%, respectively.

In the 12L / 12D group, hatching started from the fourth day and 70% hatch at the fifth day. 9L / 15D showed a similar tendency, but it was confirmed that the hatching rate was about 5% lower. In conclusion, it is concluded that 12L / 12D ~ 15L / 9D is suitable for the photoperiod suitable for raising hatching rate of over 80% of the American Royal Duck, more preferably 12L / 12D is most suitable.

<Example 3> Characterization of roughness suitable for improvement of hatching rate of the American king crab

1. Experimental Method

1) Experimental insects and numbers: 14G egg (egg scattered at 65 ° C, 65% R.H for 1 day), 2 days after egg laying, 70, 100, 50, 3 repeat

2) Experimental illumination: 600 Lux, 1,000 Lux, 1,800 Lux, 3,000 Lux

3) Survey method and period: once a day, 7 days from the beginning

4) Breeding conditions: temperature: 30 ° C, humidity: 65 ± 5%, light period: 9L / 15D

2. Experimental results

The results of the experiment are shown in Figs. 5 and 6 and Tables 5 and 6 below.

<Number of daily hatchings of the King of America in accordance with illumination> Illuminance
(Lux) Hatching days (days) Total volume
(Mari) 4 days 5 days 6 days 7 days 600 19 104 52 - 175/220 1,000 17 103 59 - 179/220 1,800 16 103 62 - 181/220 3,000 - 47 105 - 152/220

<The Daily Hatching Rate of the American King Gojari According to the Illumination> Illuminance
(Lux) Hatching days (days) Total hatching rate
(%) 4 days 5 days 6 days 7 days 600 8.6 47.3 23.6 - 79.5 1,800 7.3 46.8 28.2 - 82.3 3,600 - 21.4 47.7 - 69.1

As shown in FIGS. 5 and 6 and Tables 5 and 6, when the temperature was fixed at 30 ° C., and the total irradiated water of the American Royal Duck was measured for four illuminations of 600 Lux, 1,800 Lux, 1,000 Lux and 3,000 Lux, The highest, then 1,000 Lux, then 600 Lux, then 3,000 Lux.

In addition, there was no significant difference between 1,800 Lux and 1,000 Lux in the total wastewater, and the daily hatching rates of 600 Lux, 1,800 Lux and 3,000 Lux were examined. As a result, in case of 600 Lux and 1,800 Lux, And began to hatch within 3 days. On the other hand, 3,000 Lux hatches from 2 days after hatching from late 5th day, but hatching rate is 13.2% lower than 1,800 Lux.

In conclusion, it is considered that Lux suitable from above 600 to 1,800 Lux is suitable for raising the hatching rate of American Royal Duck, more preferably 1,800 Lux.

According to the development characteristics of the above-mentioned American king crab, the eggs of the American king crab had a hatching rate of 80 to 90% for 5 to 7 days through the above-mentioned specific hatching condition, I could.

Based on the above results, it is possible to mass produce in a short time as the optimal hatching condition of the American King's gill is clarified, and it is possible to stably plan production, thereby enabling the activation of the insect industry and the increase in income, And it is expected that labor efficiency will be increased.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined by the appended claims. It will be possible. The scope of the present invention is defined by the appended claims, and all differences within the scope of the claims are to be construed as being included in the present invention.

Claims (5)

(a) a incubation temperature of 29 ° C to 30 ° C
(b) Long-day condition 12L: 12D to 15L: 9D
(C) Rust of more than 600 to under 1,800 Under the condition of Lux,
A method of artificial hatching of a drowned American king.
The method according to claim 1,
Characterized in that the eggs of the American kingfisher are hatched at a hatched temperature of &lt; RTI ID = 0.0 &gt; 30 C. &lt;
A method of artificial hatching of a drowned American king.
The method according to claim 1,
Wherein the egg of the American kingpin is hatched under long-day conditions of 12L: 12D,
A method of artificial hatching of a drowned American king.
The method according to claim 1,
Characterized in that the eggs of the American Royal Ducks hatch under an illuminance condition of 1,800 Lux,
A method of artificial hatching of a drowned American king.
5. The method according to any one of claims 1 to 4,
Wherein the eggs of the American Royal Ducks exhibit hatching rates of 80-90% for 5-7 days.
A method of artificial hatching of a drowned American king.

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