KR20210016842A - Artificial Diet Composition for Zophobas atratus - Google Patents

Artificial Diet Composition for Zophobas atratus Download PDF

Info

Publication number
KR20210016842A
KR20210016842A KR1020190095115A KR20190095115A KR20210016842A KR 20210016842 A KR20210016842 A KR 20210016842A KR 1020190095115 A KR1020190095115 A KR 1020190095115A KR 20190095115 A KR20190095115 A KR 20190095115A KR 20210016842 A KR20210016842 A KR 20210016842A
Authority
KR
South Korea
Prior art keywords
artificial feed
bran
agarose
breeding
present
Prior art date
Application number
KR1020190095115A
Other languages
Korean (ko)
Other versions
KR102292714B1 (en
Inventor
윤형주
이경용
박해철
김성현
고현진
Original Assignee
대한민국(농촌진흥청장)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 대한민국(농촌진흥청장) filed Critical 대한민국(농촌진흥청장)
Priority to KR1020190095115A priority Critical patent/KR102292714B1/en
Publication of KR20210016842A publication Critical patent/KR20210016842A/en
Application granted granted Critical
Publication of KR102292714B1 publication Critical patent/KR102292714B1/en

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/33Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The present invention relates to an artificial feed composition for Zophobas atratus, and a method for preparing the same. More particularly, the present invention relates to an artificial feed composition for breeding, which is inexpensive to manufacture and can be provided in a form suitable for Zophobas atratus, while being able to breed Zophobas atratus with good developmental state and high breeding efficiency. Due to the development of the artificial feed composition according to the present invention, it is possible to obtain an effect of reducing production costs, to establish a mass production system with high quality artificial feed, and to secure the source technology for mass breeding of Zophobas atratus, a major insect resource, and thus an increase in the income of farmers aiming for commercialization is expected.

Description

아메리카왕거저리의 인공 사료 조성물{Artificial Diet Composition for Zophobas atratus}Artificial Diet Composition for Zophobas atratus}

본 발명은 아메리카왕거저리의 인공 사료 조성물 및 이의 제조방법에 관한 것으로, 보다 상세하게는 제조비용이 저렴하고 아메리카왕거저리에게 적합한 형태로 제공할 수 있으면서도 좋은 발육상태 및 높은 사육효율로 아메리카왕거저리를 사육할 수 있도록 하는 사육용 인공 사료 조성물에 관한 것이다.The present invention relates to an artificial feed composition and a method for producing the same, and more specifically, the production cost is inexpensive and can be provided in a form suitable for the American king geese, while providing a good development state and high breeding efficiency. It relates to an artificial feed composition for breeding to enable breeding.

아메리카왕거저리(Zophobas atratus)는 딱정벌레목에 속하는 곤충으로, 원래는 열대 중미와 남미 지역에서 서식하던 종이나 현재는 전세계에 도입되어 있다. 아메리카왕거저리는 대형 거저리로, 갈색거저리(Tenebrio molitor)보다 몸집이 매우 커 영양학적 가치가 높고, 유충 자체를 설치류, 파충류, 조류, 애완동물 및 양식어류 사료용으로도 사용할 수 있다. Zophobas atratus is an insect belonging to the order Beetle. Originally a species that lived in tropical Central and South America, but is now introduced around the world. The American king goose is a large goose. It has a higher nutritional value than the brown goose down ( Tenebrio molitor ), and the larva itself can be used as feed for rodents, reptiles, birds, pets and farmed fish.

아메리카왕거저리를 식용 또는 약용으로 활용하기 위해서는 인공증식법이 필요하다. 그러나, 아메리카왕거저리를 인공사육할 경우, 주먹이인 밀기울로 인하여 분진이 발생하여 사육자가 사육하는데 어려움이 발생할 뿐만 아니라 노동력이 많이 소모된다. 한편, 거저리과(Tenebrionidae)의 사육에 소요되는 인공 사료 등에 대한 개발 등은 미흡하며, 최근에는 곡물가격 상승으로 사료비용이 많이 소요되어 실질적으로 대량생산에 어려움을 겪고 있는 실정이다. 특히, 거저리과 중에서도 갈색거저리를 위한 인공 사료는 대한민국 공개실용신안 20-2015-0001806, 대한민국 공개특허 10-2017-0055885 등에서 보고된 바 있으나, 아메리카왕거저리를 위한 인공 사료는 지금까지 개발된 바가 없다.Artificial breeding method is required to use the American king gooseberry for edible or medicinal purposes. However, in the case of artificial breeding of American king gooseberries, dust is generated due to the bran which is a fist, which causes difficulty in breeding the breeder and consumes a lot of labor. On the other hand, the development of artificial feed, etc. for the breeding of Tenebrionidae is insufficient, and in recent years, the cost of feed is high due to the increase in grain prices, making it difficult to mass-produce. In particular, artificial feed for brown gooseberries among the gooseberries has been reported in Korean Utility Model Publication 20-2015-0001806, Korean Patent Application Publication No. 10-2017-0055885, etc., but no artificial feed for American gooseberries has been developed so far.

아메리카왕거저리는 갈색거저리와 달리 크기가 최대 5cm까지 자라 유충은 슈퍼밀웜(super mealworm)이라 불리며, 지방과 인의 함량이 높아 농가의 새로운 수익원이 될 것으로 전망되고 있다. 이에, 아메리카왕거저리에게 최적화된 형태로 제공되면서도 우수한 사육효율을 달성할 수 있는 인공 사료에 대한 연구개발이 필요한 실정이다. Unlike brown gooseberries, American king gooseberries grow up to 5cm in size, and the larvae are called super mealworms, and their high content of fat and phosphorus is expected to become a new source of profit for farmers. Accordingly, there is a need for research and development on artificial feed that can achieve excellent breeding efficiency while being provided in an optimized form to American king geese.

대한민국 공개실용신안 20-2015-0001806Republic of Korea public utility model 20-2015-0001806 대한민국 공개특허 10-2017-0055885Republic of Korea Patent Publication 10-2017-0055885

이에, 본 발명자들은 아메리카왕거저리를 위한 인공 사료 조성물을 개발하고자 노력한 결과, 밀기울(wheat bran), 밀기울의 대체 먹이원으로 동물성 단백질인 어분(fishmeal) 및 아가로스(agarose)를 혼합하여 생산비 단가를 줄이면서도 아메리카왕거저리에게 최적화된 형태의 인공 사료 조성물을 제조하고, 상기 인공 사료 조성물 급이 시 좋은 발육상태 및 높은 사육효율로 아메리카왕거저리를 사육할 수 있음을 확인하여 본 발명을 완성하였다. Accordingly, as a result of the present inventors trying to develop an artificial feed composition for the American king gourd, wheat bran, fishmeal and agarose, which are animal proteins as an alternative food source for bran, are mixed to reduce the production cost. The present invention was completed by confirming that the artificial feed composition in a form optimized for the American king goose down was prepared, and when the artificial feed composition was fed, it was possible to breed the American king goose down with good growth state and high breeding efficiency.

따라서 본 발명의 목적은 밀기울, 어분 및 아가로스를 포함하는 아메리카거저리 사육용 인공 사료 조성물을 제공하는 것이다.Accordingly, it is an object of the present invention to provide an artificial feed composition for rearing American mealworms comprising bran, fish meal and agarose.

본 발명의 또 다른 목적은 밀기울, 어분 및 아가로스를 포함하는 아메리카왕거저리 사육용 인공 사료 조성물의 제조 방법을 제공하는 것이다.Another object of the present invention is to provide a method for producing an artificial feed composition for breeding red snappers comprising bran, fish meal and agarose.

본 발명의 또 다른 목적은 상기 아메리카왕거저리 사육용 인공 사료 조성물을 아메리카왕거저리에 급이하여 아메리카왕거저리를 사육하는 방법을 제공하는 것이다.Another object of the present invention is to provide a method for breeding American king geese by feeding the artificial feed composition for rearing American king geese.

상기 목적을 달성하기 위하여, 본 발명은 밀기울, 어분 및 아가로스를 포함하는 아메리카왕거저리 사육용 인공 사료 조성물을 제공한다.In order to achieve the above object, the present invention provides an artificial feed composition for breeding American king geese comprising bran, fish meal and agarose.

또한, 본 발명은 밀기울, 어분 및 아가로스를 포함하는 아메리카왕거저리 사육용 인공 사료 조성물의 제조 방법을 제공한다.In addition, the present invention provides a method for producing an artificial feed composition for breeding red snapper, including bran, fish meal and agarose.

또한, 본 발명은 상기 아메리카왕거저리 사육용 인공 사료 조성물을 아메리카왕거저리에 급이하여 아메리카 왕거저리를 사육하는 방법을 제공한다.In addition, the present invention provides a method for breeding American king geese by feeding the artificial feed composition for rearing American king geese.

본 발명에 의한 인공 사료 조성물의 개발로 인해 생산비 절감효과를 얻게됨과 동시에 양질의 인공 사료로 대량생산 시스템 확립 및 주요 곤충자원인 아메리카왕거저리의 대량사육 원천기술 확보가 가능하므로, 상업화를 목적으로 하는 농가의 소득향상이 기대된다.Due to the development of the artificial feed composition according to the present invention, the production cost reduction effect is obtained, and at the same time, it is possible to establish a mass production system with high-quality artificial feed and to secure the original technology for mass breeding of the main insect resource. Farmers' income is expected to improve.

도 1은 인공사료 종류에 따른 아메리카왕거저리 유충의 단계별(1-10령) 발육기간을 나타낸 도이다.
도 2는 인공사료 종류에 따른 아메리카왕거저리 유충의 령별 체중을 나타낸 도이다.
도 3은 인공사료 종류에 따른 아메리카왕거저리의 폐사율을 나타낸 도이다.
도 4는 인공사료(밀기울, 어분) 비율에 따른 아메리카왕거저리 유충의 단계별(1-10령) 발육기간을 나타낸 도이다.
도 5는 인공사료(밀기울, 어분) 비율에 따른 아메리카왕거저리 유충의 령별 체중을 나타낸 도이다.
도 6은 인공사료(밀기울, 어분) 비율에 따른 아메리카왕거저리의 폐사율을 나타낸 도이다.
도 7은 인공사료(밀기울, 어분, 아가로스)의 아가로스 비율에 따른 아메리카왕거저리 유충의 단계별(1-10령) 발육기간을 나타낸 도이다.
도 8은 인공사료(밀기울, 어분, 아가로스)의 아가로스 비율에 따른 아메리카왕거저리 유충의 령별 체중을 나타낸 도이다.
도 9는 인공사료(밀기울, 어분, 아가로스)의 아가로스 비율에 따른 아메리카왕거저리의 폐사율을 나타낸 도이다.
도 10은 본원 발명의 인공 사료 조성물 급이에 따른 아메리카왕거저리 유충의 단계별 발육기간을 나타낸 도이다.
도 11은 본원 발명의 인공 사료 조성물 급이에 따른 아메리카왕거저리 유충의 령별 체중을 나타낸 도이다.
도 12는 본원 발명의 인공 사료 조성물 급이에 따른 아메리카왕거저리의 폐사율을 나타낸 도이다.
도 13은 본원 발명의 인공 사료 조성물 급이에 따른 아메리카왕거저리 발육단계별 전용시기를 나타낸 도이다.
도 14는 본원 발명의 인공 사료 조성물 급이에 따른 아메리카왕거저리 발육단계별 전용기간을 나타낸 도이다.
도 15는 본원 발명의 인공 사료 조성물 급이에 따른 아메리카왕거저리의 성충 우화율 및 1령~성충우화까지의 기간을 나타낸 도이다.
도 16은 본원 발명의 인공 사료 조성물 급이에 따른 아메리카왕거저리의 1마리당 체중(mg) 및 먹이 소모량(mg)을 나타낸 도이다.
도 17은 본원 발명의 인공 사료 조성물 급이에 따른 사료효율(ECD)을 나타낸 도이다.
1 is a diagram showing the staged (1-10 in.) growth period of the American larva according to the type of artificial feed.
Figure 2 is a diagram showing the weight by age of the larvae of American king geeseol according to the type of artificial feed.
Figure 3 is a diagram showing the mortality rate of American king geeseori according to the type of artificial feed.
Figure 4 is a diagram showing the stage (1-10 years old) development period of the larvae of the American king geese according to the ratio of artificial feed (wheat bran, fish meal).
Figure 5 is a diagram showing the weight of the larvae by age according to the ratio of artificial feed (wheat bran, fish meal).
Figure 6 is a diagram showing the mortality rate of American king gooseberries according to the ratio of artificial feed (bran, fish meal).
Figure 7 is a diagram showing the stage (1-10 years old) growth period of the larvae of the American kingfish according to the agarose ratio of artificial feed (bran, fish meal, agarose).
Figure 8 is a diagram showing the weight of the larvae by age according to the agarose ratio of artificial feed (bran, fish meal, agarose).
9 is a diagram showing the mortality rate of American king gooseberries according to the agarose ratio of artificial feed (bran, fish meal, agarose).
Figure 10 is a diagram showing the step-by-step growth period of the larvae of American king geese according to the artificial feed composition feeding of the present invention.
Figure 11 is a view showing the weight of the larvae of the American king geeseori according to the artificial feed composition feeding of the present invention.
Figure 12 is a diagram showing the mortality of the American king geeseori according to the artificial feed composition feeding of the present invention.
13 is a diagram showing a dedicated time for each stage of development of the American king geese according to the artificial feed composition feeding of the present invention.
14 is a diagram showing the exclusive period for each stage of development of the American king geese according to the artificial feed composition feeding of the present invention.
FIG. 15 is a diagram showing the adult allegory rate and the period from 1 in. to adult allegory according to the feeding of the artificial feed composition of the present invention.
Figure 16 is a diagram showing the body weight (mg) and food consumption (mg) per animal according to the artificial feed composition feeding of the present invention.
17 is a diagram showing the feed efficiency (ECD) according to the artificial feed composition feeding of the present invention.

본 발명은 밀기울, 어분 및 아가로스를 포함하는 아메리카왕거저리 사육용 인공 사료 조성물을 제공한다. The present invention provides an artificial feed composition for breeding red snapper, comprising bran, fish meal and agarose.

이하, 본 발명을 상세히 설명한다. Hereinafter, the present invention will be described in detail.

본 명세서에서 달리 정의되지 않은 용어들은 본 발명이 속하는 기술분야에서 통상적으로 사용되는 의미를 갖는 것이다.Terms not otherwise defined in the specification have the meanings commonly used in the art to which the present invention belongs.

본 발명에서 용어, “밀기울(wheat bran)”은 소맥피라고도 하며 밀을 빻아 체로 쳐서 남은 찌꺼기로 일반적으로 사료로 많이 사용된다.In the present invention, the term “wheat bran” is also referred to as wheat bran, and is a residue left after grinding wheat and sifting, and is generally used as a feed.

본 발명에서 용어, “어분(fish meal)”은 배합사료의 주요 단백질 공급원으로 외해의 소형 어류를 갈아 건조 분말 형태로 만든 것으로 단백질 함량이 높아 본 발명에서 밀기울 대체먹이원으로 사용하였다.In the present invention, the term “fish meal” is a major protein source of mixed feed, which is made from small fish in the open sea into a dry powder form, and has a high protein content, so it was used as an alternative feeding source for bran in the present invention.

본 발명에서 용어, “아가로스(agarose)”는 해조류 유래 다당류인 한천(agar)의 유효성분으로, 갈락토스(D-galactose)와 3,6-안하이드로-L-갈락토스(3,6-anhydro-L-galactose)가 β-1,4 형태로 결합한 단위체인 아가로비오스(agarobiose)가 반복되어 α-1,3 결합으로 연결된 직쇄구조로 되어있고 겔(gel)화력이 강한 물질이다. 한편, 본 발명에 있어서, 아가로스(agarose)는 아가로스 대신 한천을 사용할 수도 있다.In the present invention, the term "agarose" is an active ingredient of agar, a polysaccharide derived from algae, and galactose (D-galactose) and 3,6-anhydro-L-galactose (3,6-anhydro- L-galactose) has a linear structure connected by α-1,3 bonds by repeating agarobiose, a unit in which β-1,4 forms are bonded, and has strong gel-forming power. On the other hand, in the present invention, agarose may use agar instead of agarose.

본 발명에 따른 아메리카왕거저리 사육용 인공 사료 조성물은 밀기울, 어분과 함께 아가로스를 혼합함에 따라 사료 급이 시 분진이 발생하지 않으므로 사육자의 분진에 대한 노출을 최소화하고, 유용곤충 사육과정에서 문제되는 분진을 효율적으로 제거할 수 있다.The artificial feed composition for rearing red snapper according to the present invention does not generate dust when feeding the feed by mixing agarose with bran and fish meal, thus minimizing the exposure of the breeder to dust and causing problems in the process of breeding useful insects. Dust can be removed efficiently.

또한, 본 발명에 따른 아메리카왕거저리 사육용 인공 사료 조성물은 아가로스를 포함함에 따라 인공 사료를 주고 먹이기에 쉬운 고체형으로 제조함으로써, 거저리과(Tenebrionidae) 중 대형 거저리에 속하는 아메리카왕거저리 급이에 최적화된 것에 특징이 있다. In addition, the American king geojeori artificial feed composition for breeding according to the invention are agarose by preparing an easy Solid form the feed to give the artificial diet as including Ross, geojeorigwa (Tenebrionidae) of the optimization in the American king geojeori class belong to the large geojeori There is a characteristic of what has been done.

나아가, 본 발명에 따른 아메리카왕거저리 사육용 인공 사료 조성물은 상기 밀기울, 어분 및 아가로스를 최적 비율로 포함하여 아메리카왕거저리의 발육효율 및 사육효율을 최대화 한 것에 발명의 특징이 있다.Further, the artificial feed composition for rearing American king geese according to the present invention includes the bran, fish meal, and agarose in an optimal ratio to maximize the growth efficiency and breeding efficiency of American king geese.

본 발명에 있어서, 밀기울, 어분 및 아가로스는 밀기울:어분:아가로스=9:1:0.5 내지 2(w/w)의 비율로 혼합될 수 있으며, 바람직하게는 밀기울:어분:아가로스=9:1:1 내지 2(w/w)의 비율로 혼합될 수 있고, 보다 바람직하게는 밀기울:어분:아가로스=9:1:1 내지 1.5(w/w)의 비율로 혼합될 수 있고, 보다 더 바람직하게는 밀기울:어분:아가로스=9:1:1 내지 1.25(w/w)의 비율로 혼합될 수 있고, 가장 바람직하게는 밀기울:어분:아가로스=9:1:1(w/w)의 비율로 혼합될 수 있다. 본 발명의 일 실시예에서는, 밀기울:어분:아가로스=9:1:1 내지 2(w/w) 비율로 혼합하는 경우 밀기울로만 사육할 때보다 유충발육기간이 단축되고, 밀기울:어분:아가로스=9:1:1 내지 1.5(w/w) 비율로 혼합하는 경우 유충 체중이 현저히 증가하고, 나아가 밀기울:어분:아가로스=9:1:1(w/w) 비율로 혼합하는 경우 폐사율이 현저히 감소함을 확인하였다.In the present invention, bran, fish meal and agarose may be mixed in a ratio of bran: fish meal: agarose = 9: 1: 0.5 to 2 (w / w), preferably bran: fish meal: agarose = 9 It may be mixed in a ratio of :1:1 to 2 (w/w), more preferably bran:fishmeal:agarose=9:1:1 to 1.5 (w/w), Even more preferably, it may be mixed in a ratio of bran: fish meal: agarose=9:1:1 to 1.25 (w/w), and most preferably bran:fishmeal:agarose=9:1:1 (w /w) can be mixed. In an embodiment of the present invention, when mixing in a ratio of bran: fish meal: agarose = 9: 1: 1 to 2 (w/w), the larva development period is shorter than when rearing only bran, and bran: fish meal: baby When mixed in a ratio of ross = 9: 1 to 1 to 1.5 (w / w), the weight of larvae significantly increases, and furthermore, mortality when mixed in a ratio of bran: fish meal: agarose = 9: 1: 1 (w/w) It was confirmed that this significantly decreased.

본 발명에 있어서, 상기 인공 사료 조성물은 첨가제로서 소르빈산(sorbic acid), 메틸파라벤(Methyl-p-hyroxybenzoate)을 더 포함할 수 있으며, 나아가 아메리카왕거저리 사육에 필요한 식용유, 종합비타민 및 증류수(DW)를 더 포함할 수 있다.In the present invention, the artificial feed composition may further include sorbic acid and methyl-p-hyroxybenzoate as additives, and further, edible oil, multivitamin, and distilled water (DW) required for breeding red snappers It may further include.

또한, 본 발명은 밀기울, 어분 및 아가로스를 9:1:0.5 내지 2(w/w)로 혼합하는 단계;를 포함하는 아메리카왕거저리 사육용 인공 사료 조성물의 제조 방법을 제공한다.In addition, the present invention provides a method for producing an artificial feed composition for breeding red snapper including; mixing bran, fish meal and agarose in 9:1:0.5 to 2 (w/w).

본 발명에 있어서, 상기 제조 방법은 소르빈산, 메틸파라벤, 식용유, 종합비타민 및 증류수를 더 포함하여 혼합하는 단계;를 더 포함할 수 있다.In the present invention, the manufacturing method may further include a step of mixing sorbic acid, methyl paraben, cooking oil, multivitamin, and distilled water further.

본 발명에 있어서, 상기 혼합은 혼합기를 사용하여 혼합하는 것일 수 있으며, 당 분야에 공지된 방법을 제한 없이 사용하여 유효성분을 혼합해 본 발명에 따른 인공 사료 조성물을 제조할 수 있다.In the present invention, the mixing may be mixing using a mixer, and the active ingredient may be mixed using a method known in the art without limitation to prepare the artificial feed composition according to the present invention.

또한, 본 발명은 본 발명에 따른 아메리카왕거저리 사육용 인공 사료 조성물을 아메리카왕거저리에 급이하여 아메리카왕거저리를 사육하는 방법을 제공한다.In addition, the present invention provides a method for breeding American king geese by feeding the artificial feed composition for breeding American king geese according to the present invention.

본 발명에 있어서, 아메리카왕거저리는 일반적인 곤충사육조건에서 사육할 수 있으며, 예를 들어 사육실 환경을 25 내지 33℃, 습도 65±5%, 광주기 9L/15D, 조도 1800lux로 설정하여 사육할 수 있고, 바람직하게는 30℃, 습도 65±5%, 광주기 9L/15D, 조도 1800lux로 설정하여 사육할 수 있다.In the present invention, the red snapper can be reared under general insect breeding conditions, for example, it can be reared by setting the breeding room environment to 25 to 33°C, humidity 65±5%, photoperiod 9L/15D, illuminance 1800lux. And, preferably, it can be reared by setting at 30°C, humidity 65±5%, photoperiod 9L/15D, and illumination 1800lux.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

실시예 1. 인공 사료 종류에 따른 발육특성Example 1. Development characteristics according to artificial feed type

1-1. 인공 사료 종류 선별1-1. Selection of artificial feed types

아메리카왕거저리 인공 사료에 들어가는 구체적인 구성성분을 선별하기 위해, 밀기울(wheat bran), 동물성 단백질인 어분(fish meal) 및 인삼잎(ginseng leaf)을 하기 표 1에 기재된 조성비대로 혼합하여 인공 사료 조성물을 제조하였다.In order to select specific constituents that enter the artificial feed, wheat bran, animal protein fish meal, and ginseng leaf are mixed according to the composition ratio shown in Table 1 below to prepare an artificial feed composition. Was prepared.

WF2WF2 WF6WF6 WFG1WFG1 WFG2WFG2 대조군Control 밀기울(g)Bran (g) 9090 7070 7070 7070 100100 어분(g)Fish meal (g) 1010 30 30 25 25 20 20 -- 인삼잎(g)Ginseng leaves (g) -- -- 55 1010 --

모든 실험군(WF2, WF6, WFG1, WFG2)은 밀기울(우리밀 가공공장; 전남 구례), 어분(동아사료원) 및 인삼잎(충남농업기술원 인삼약초연구소)을 혼합한 후 소르빈산(sorbic acid), 메틸파라벤(Methyl-p-hyroxybenzoate) 등의 첨가물과 식용유, 종합비타민(네오비타, neo-vita; 삼우메디안) 및 증류수를 하기 표 2에 기재된 조성비대로 더 포함하여 제조하였다. 상기 종합비타민은 비타민-A, 비타민-D3(콜레칼시페롤), 비타민-E(토코페롤 아세테이트), 비타민-K3(메나디온아황산수소나트륨), 비타민-B1(티아민염산염), 비타민-B2(리보플라빈), 비타민-B6(피리독신염산염), 비타민-B12(시아노코발라민), 비타민-C(아스코르브산), 폴산, 니코틴산, 판토텐산칼슘, DL-메티오닌 및 부형제(포도당[KP])를 포함하는 것으로 시판되는 것을 사용하였다.All experimental groups (WF2, WF6, WFG1, WFG2) were mixed with bran (woori wheat processing plant; Gurye, Jeollanam-do), fish meal (Donga Feed Center), and ginseng leaf (Ginseng Herb Research Institute of Chungnam Agricultural Technology Institute), and then sorbic acid, methyl Additives such as paraben (Methyl-p-hyroxybenzoate), cooking oil, multivitamin (neo-vita, neo-vita; Samwoo median), and distilled water were further included in the composition ratios shown in Table 2 below. The multivitamins are vitamin-A, vitamin-D3 (cholecalciferol), vitamin-E (tocopherol acetate), vitamin-K3 (sodium menadione sulfite), vitamin-B1 (thiamine hydrochloride), vitamin-B2 (riboflavin) ), vitamin-B6 (pyridoxine hydrochloride), vitamin-B12 (cyanocobalamin), vitamin-C (ascorbic acid), folic acid, nicotinic acid, calcium pantothenate, DL-methionine and excipients (glucose [KP]). Was used.

함량content 소르빈산(g)Sorbic acid (g) 0.20.2 메틸파라벤(g)Methylparaben (g) 0.20.2 식용유(ml)Cooking oil (ml) 22 종합비타민(g)Multivitamin (g) 1010 DW(ml)DW(ml) 400400

구체적인 인공 사료의 제조방법은 하기와 같다:The specific method for producing artificial feed is as follows:

(대량으로 제조하는 경우) 종합비타민을 제외한 구성성분을 잘 혼합하여 철제도시락 등의 용기에 넣는 단계(제1 단계); 파라필름이나 랩으로 덮은 후 고압멸균기(autoclave)에서 100 내지 110℃ 조건으로 10 내지 20분 동안 찌는 단계(제2 단계); 상기 증자된 인공 사료에 70% 에탄올에 녹인 종합비타민을 넣고 골고루 섞어 인공사료를 눌러주는 단계(제3 단계); 및 실온에서 물 등으로 냉각시키는 단계(제4 단계). 상기 제조된 인공 사료 조성물은 사용하기 전까지 5℃의 냉장고에 넣어 보관하였다.(In the case of manufacturing in large quantities) the step of mixing the components excluding multivitamins well and putting them in a container such as an iron lunch box (first step); After covering with parafilm or wrap, steaming for 10 to 20 minutes at 100 to 110°C in an autoclave (second step); Adding multivitamin dissolved in 70% ethanol to the increased artificial feed, mixing evenly, and pressing the artificial feed (third step); And cooling with water or the like at room temperature (step 4). The prepared artificial feed composition was stored in a refrigerator at 5°C until use.

(소량으로 제조하는 경우) 삼각플라스크 등에 증류수를 붓고 아가(Agar)를 넣은 뒤 전자레인지에서 3분간 가열하는 단계(제1 단계); 상기 가열한 아가에 종합비타민을 제외한 구성성분을 모두 넣고 핫플레이트(200℃에서 녹이는 단계(제2 단계); 상기 핫플레이트에서 3분 이상 저어가며 끓이다가 핫플레이드를 끈 후 잠시 냉각하는 단계(제3 단계); 상기 인공 사료에 70% 에탄올에 녹인 종합비타민을 넣고 골고루 섞어 용기에 붓는 단계(제4 단계). 상기 제조된 인공 사료 조성물은 사용하기 전까지 5℃의 냉장고에 넣어 보관하였다.(In the case of manufacturing in small amounts) pouring distilled water into an Erlenmeyer flask, adding agar, and heating in a microwave for 3 minutes (first step); Putting all the ingredients except for the multivitamin in the heated agar, and dissolving it at 200°C (second step); stirring the hot plate for at least 3 minutes while boiling, turning off the hot plate, and cooling it for a while ( Step 3): Add multivitamin dissolved in 70% ethanol to the artificial feed, mix evenly and pour it into a container (Step 4) The prepared artificial feed composition was stored in a refrigerator at 5°C until use.

1-2. 발육특성 확인1-2. Confirmation of development characteristics

상기 실시예 1-1에서 제조한 인공 사료 조성물을 아메리카왕거저리 유충에 급이하여 령별 발육기간, 령별 체중 및 폐사율을 측정하였다. 대조군으로는 동량의 밀기울(밀기울 100%)을 사용하였다. 사육실 환경조건은 30℃, 습도 65%, 광주기 9L/15D, 조도 1800lux로 설정하였으며, 아메리카왕거저리 유충을 40마리씩 5구를 설정(총 200마리)하여 3반복으로 실험을 수행하였다. 그 결과를 도 1 내지 3에 나타내었다.The artificial feed composition prepared in Example 1-1 was fed to the larvae of larvae to measure the growth period by age, body weight and mortality by age. The same amount of bran (100% bran) was used as a control. The environmental conditions of the breeding room were set at 30°C, humidity 65%, photoperiod 9L/15D, and illumination 1800lux, and the experiment was performed in 3 repetitions by setting 5 heads of 40 larvae each (total 200). The results are shown in FIGS. 1 to 3.

도 1에 나타낸 바와 같이, 4종 인공 사료의 1-10령까지의 발육기간은 WF6이 53.4±7.5일, WF2가 53.9±7.4일로 대조군인 밀기울(53.1±5.0일)보다 0.3-0.8일이 긴 것을 확인하였다. 반면, 인삼잎 가루가 첨가된 WFG1(58.3±7.0일), WFG2(55.9±6.2일)는 대조군보다 2.8-5.2일 발육기간이 길었다.As shown in Fig. 1, the growth period from 1 to 10 years of age of 4 types of artificial feed was 53.4±7.5 days for WF6 and 53.9±7.4 days for WF2, which was 0.3-0.8 days longer than that of the control bran (53.1±5.0 days). Confirmed. On the other hand, WFG1 (58.3±7.0 days) and WFG2 (55.9±6.2 days) with ginseng leaf powder added 2.8-5.2 days longer than the control group.

체중의 경우, 도 2에 나타낸 바와 같이, 10령 기준으로 4종의 인공 사료 WF2(58.7±43.4mg), WF6(74.9±57.1mg), WFG1(72.9±65.4mg) 및 WFG2(46.6±20.8mg)가 대조군(26.1±12.3mg)보다 1.8-2.9배 증가함을 확인하였다(P=0.0001). 또한, 4종의 인공 사료 간의 비교 결과, WF6(대조군 대비 2.9배 증가), WFG1(2.8배 증가), WF2(2.3배 증가), WFG2(1.8배 증가) 순으로 체중이 증가함을 확인하였다. In the case of body weight, as shown in Fig.2, based on 10 years of age, four kinds of artificial feeds WF2 (58.7±43.4mg), WF6 (74.9±57.1mg), WFG1 (72.9±65.4mg) and WFG2 (46.6±20.8mg) ) Was found to be 1.8-2.9 times higher than that of the control group (26.1±12.3mg) (P=0.0001). In addition, as a result of comparison between the four artificial feeds, it was confirmed that the weight increased in the order of WF6 (2.9 times increase compared to the control group), WFG1 (2.8 times increase), WF2 (2.3 times increase), and WFG2 (1.8 times increase).

한편, 12령까지 폐사율을 비교 조사한 결과, 도 3에 나타낸 바와 같이 대조군과 WF2는 폐사율이 10%에 그치는데 반해 WF6은 38%, WFG1은 55%, WFG2는 폐사율이 53%로 대조군에 비하여 28-45%나 높은 폐사율을 나타냄을 확인하였다.On the other hand, as a result of comparing and examining the mortality rate up to 12 years old, as shown in FIG. 3, the mortality rate for the control group and WF2 was only 10%, whereas 38% for WF6, 55% for WFG1, and 53% for WFG2 were 28 compared to the control group. It was confirmed that the mortality rate was as high as -45%.

상기 결과를 통해, 인삼잎이 첨가된 WFG1, WFG2는 대조군보다 체중은 무거우나, 발육기간이 길고, 폐사율이 높아 아메리카왕거저리의 인공사료로서는 적합하지 않음을 확인하였다. 이에, 이하 실험에서는 WF2 및 WF6을 기반으로 인공 사료 조성물을 제작하여 실험에 사용하였다.Through the above results, it was confirmed that WFG1 and WFG2 to which ginseng leaves were added were heavier than the control group, but had a longer development period and high mortality rate, so that they were not suitable as artificial feeds for American king gooseberries. Thus, in the following experiment, an artificial feed composition was prepared based on WF2 and WF6 and used in the experiment.

실시예 2. 밀기울, 어분의 최적 혼합 비율 탐색Example 2. Exploration of optimal mixing ratio of bran and fish meal

2-1. 인공 사료 조성물 제조2-1. Manufacturing of artificial feed composition

양질의 아메리카왕거저리 사육을 위해 밀기울 및 어분의 최적 혼합 비율을 탐색하였다. 구체적으로, 밀기울 및 어분을 하기 표 3에 기재된 조성비대로 혼합하여 인공 사료 조성물을 제조하였다. 모든 실험군은 밀기울 및 어분을 혼합한 후 소르빈산, 메틸파라벤 등의 첨가물과 식용유, 종합비타민(네오비타, neo-vita) 및 증류수를 상기 표 2에 기재된 조성비대로 더 포함하여 제조하였다.The optimal mixing ratio of bran and fishmeal was searched for breeding of good quality king gooseberries. Specifically, an artificial feed composition was prepared by mixing bran and fish meal according to the composition ratio shown in Table 3 below. All experimental groups were prepared by mixing bran and fish meal, and further comprising additives such as sorbic acid and methylparaben, cooking oil, multivitamins (neovita, neo-vita), and distilled water according to the composition ratios shown in Table 2.

WF2WF2 WF6WF6 대조군Control 밀기울(g)Bran (g) 9090 7070 100100 어분(g)Fish meal (g) 1010 3030 --

2-2. 발육특성 확인2-2. Confirmation of development characteristics

상기 실시예 2-1에서 제조한 인공 사료 조성물을 아메리카왕거저리 유충에 급이하여 령별 발육기간, 령별 체중 및 폐사율을 측정하였다. 대조군으로는 동량의 밀기울(밀기울 100%)을 사용하였다. 사육실 환경조건은 30℃, 습도 65%, 광주기 9L/15D, 조도 1800lux로 설정하였으며, 아메리카왕거저리 유충을 40마리씩 3구를 설정(총 120마리)하여 3반복으로 실험을 수행하였다. 그 결과를 도 4 내지 6에 나타내었다.The artificial feed composition prepared in Example 2-1 was fed to the larvae of the larva to measure the growth period by age, body weight by age, and mortality. As a control, the same amount of wheat bran (100% wheat bran) was used. The environmental conditions of the breeding room were set at 30°C, humidity 65%, photoperiod 9L/15D, and illumination 1800lux, and the experiment was performed in 3 repetitions by setting 3 bulbs of 40 larvae each (a total of 120). The results are shown in FIGS. 4 to 6.

도 4에 나타낸 바와 같이, 2종 인공 사료의 1-10령까지 발육기간은 WF6이 53.4±7.5일, WF2는 53.9±7.4일로 대조군인 밀기울(53.1±5.0일)보다 0.3-0.8일이 긴 것을 확인하였다(P=0.854).As shown in Fig. 4, the growth period from 1 to 10 years of age of 2 types of artificial feed was 53.4±7.5 days for WF6 and 53.9±7.4 days for WF2, which was 0.3-0.8 days longer than that of the control bran (53.1±5.0 days). Confirmed (P=0.854).

체중의 경우, 도 5에 나타낸 바와 같이, 10령 기준으로 인공 사료 WF6(74.9±57.1mg)가 대조군(26.1±12.3mg)보다 2.9배 증가하고, WF2(58.7±43.4mg)도 2.3배 증가하였음을 확인하였다(P=0.0001). In the case of body weight, as shown in Fig. 5, the artificial feed WF6 (74.9±57.1mg) increased 2.9 times than that of the control group (26.1±12.3mg) and WF2 (58.7±43.4mg) increased 2.3 times at the age of 10, as shown in FIG. Was confirmed (P=0.0001).

한편, 12령까지 폐사율을 비교 조사한 결과, 도 6에 나타낸 바와 같이 대조군과 WF2는 10%에 그치는데 반해 WF6은 38%로 대조군에 비하여 28%나 높게 나타남을 확인하였다.On the other hand, as a result of a comparative investigation of mortality up to age 12, it was confirmed that the control and WF2 were only 10%, whereas the WF6 was 38%, 28% higher than that of the control, as shown in FIG.

상기 결과를 통해, WF6은 WF2보다 체중은 0.6배 무거우나, 폐사율이 현저히 높은 단점이 있어, 아메리카왕거저리의 인공 사료로서는 WF2가 WF6보다 적합함을 확인하였다. 이에, 이하 실험에서는 WF2를 기반으로 인공 사료 조성물을 제작하여 실험에 사용하였다.Through the above results, it was confirmed that WF6 is 0.6 times heavier than WF2, but has a disadvantage in that the mortality rate is remarkably high, so that WF2 is more suitable than WF6 as an artificial feed for American king geese. Thus, in the following experiment, an artificial feed composition was prepared based on WF2 and used in the experiment.

실시예 3. 밀기울, 어분, 아가로스의 최적 혼합 비율 탐색Example 3. Exploring the optimal mixing ratio of bran, fish meal, and agarose

3-1. 인공 사료 조성물 제조3-1. Manufacturing of artificial feed composition

아메리카왕거저리의 인공 사료 급이에 따른 폐사율을 최소화하기 위하여, 인공 사료 조성물에 아가로스(AGAR, NF/ECC, 100 Mesh; Bio-Serv)를 첨가하여 아가로스 농도별 발육특성을 알아보고자 하였다. 구체적으로, 밀기울, 어분 및 아가로스를 하기 표 4에 기재된 조성비대로 혼합하여 인공 사료 조성물을 제조하였다. 모든 실험군은 밀기울, 어분 및 아가로스를 혼합한 후 소르빈산, 메틸파라벤 등의 첨가물과 식용유, 종합비타민(네오비타, neo-vita) 및 증류수를 상기 표 2에 기재된 조성비대로 더 포함하여 제조하였다.In order to minimize the mortality rate due to the artificial feed feeding of the red snapper, agarose (AGAR, NF/ECC, 100 Mesh; Bio-Serv) was added to the artificial feed composition to investigate the growth characteristics of each agarose concentration. Specifically, an artificial feed composition was prepared by mixing bran, fish meal and agarose in the composition ratio shown in Table 4 below. All experimental groups were prepared by mixing bran, fish meal and agarose, and further comprising additives such as sorbic acid and methylparaben, cooking oil, multivitamin (neo-vita, neo-vita), and distilled water at the composition ratios shown in Table 2.

WFA1WFA1 WFA2WFA2 WFA3WFA3 WFA4WFA4 대조군Control 밀기울(g)Bran (g) 9090 9090 9090 9090 100100 어분(g)Fish meal (g) 1010 10 10 1010 1010 -- 아가로스(g)Agarose (g) 55 1010 1515 2020 --

3-2. 발육특성 확인3-2. Confirmation of development characteristics

상기 실시예 3-1에서 제조한 인공 사료 조성물을 아메리카왕거저리 유충에 급이하여 령별 발육기간, 령별 체중 및 폐사율을 측정하였다. 대조군으로는 동량의 밀기울(밀기울 100%)을 사용하였다. 사육실 환경조건은 30℃, 습도 65%, 광주기 9L/15D, 조도 1800lux로 설정하였으며, 아메리카왕거저리 유충을 30마리씩 5구를 설정(총 150마리)하여 3반복으로 실험을 수행하였다. 그 결과를 도 7 내지 9에 나타내었다.The artificial feed composition prepared in Example 3-1 was fed to the larvae of larvae to measure the growth period by age, body weight and mortality by age. As a control, the same amount of wheat bran (100% wheat bran) was used. The environmental conditions of the breeding room were set at 30°C, humidity 65%, photoperiod 9L/15D, and illumination 1800lux, and the experiment was carried out in 3 repetitions by setting 5 bulbs of 30 larvae each (total of 150). The results are shown in FIGS. 7 to 9.

도 7에 나타낸 바와 같이, 4종 인공 사료의 1-10령까지 발육기간은 밀기울:어분:아가로스=9:1:2(w/w) 비율로 혼합한 WFA4가 48.8±5.8일로 가장 짧았고, WFA3(50.2±4.9일), WFA2(51.0±4.9일), WFA3(52.6±5.0일) 순으로 짧게 나타남을 확인하였다. 아가로스 포함 인공 사료는 대조군인 밀기울(53.2±5.0일)보다 발육기간이 평균 0.6-4.4일이 짧음을 확인하였다(5령, P=0.036; 10령, P=0.055). As shown in Fig. 7, the growth period of 4 kinds of artificial feeds up to 1-10 years old was the shortest at 48.8±5.8 days with WFA4 mixed in a ratio of bran:fishmeal:agarose=9:1:2(w/w), It was confirmed that WFA3 (50.2±4.9 days), WFA2 (51.0±4.9 days), and WFA3 (52.6±5.0 days) appeared briefly in this order. The artificial feed containing agarose was found to have a shorter development period of 0.6-4.4 days on average than the control bran (53.2±5.0 days) (5 years old, P=0.036; 10 years old, P=0.055).

체중의 경우, 도 8에 나타낸 바와 같이, 아가로스 포함 인공 사료가 대조군인 밀기울보다 높음을 확인하였다. 구체적으로, 15령 기준으로 대조군(272.9±142.4mg)보다 1.2-1.6배 증가하였고(P=0.037), 4종 인공 사료의 비교결과, 밀기울:어분:아가로스=9:1:1.5(w/w) 비율로 혼합한 WFA3이 448.1±216.4mg으로 가장 무거웠고(대조군 대비 1.64배 증가), WFA2(1.30배 증가), WFA4(1.28배 증가), WFA1(1.23배 증가) 순으로 체중이 증가함을 확인하였다.In the case of body weight, as shown in Fig. 8, it was confirmed that the artificial feed containing agarose was higher than that of the control bran. Specifically, it increased 1.2-1.6 times compared to the control group (272.9±142.4mg) at the age of 15 (P=0.037), and as a result of comparison of 4 types of artificial feed, bran:fishmeal:agarose=9:1:1.5(w/ w) WFA3 mixed at the ratio was the heaviest at 448.1±216.4mg (1.64 times increase compared to the control group), WFA2 (1.30 times increase), WFA4 (1.28 times increase), and WFA1 (1.23 times increase) in order of weight gain. Was confirmed.

한편, 14령까지 폐사율을 비교 조사한 결과, 도 9에 나타낸 바와 같이 대조군과 밀기울:어분:아가로스=9:1:1(w/w) 비율로 혼합한 WFA2는 각각 20.0% 및 23.3%인데 반하여 WFA1은 폐사율이 46.7%로 가장 높고, WFA3(43.3%), WFA4(33.3%) 순으로 대조군에 비해서 3.3-26.7%나 높은 폐사율을 보임을 확인하였다.On the other hand, as a result of comparing and examining mortality up to 14 years old, as shown in FIG. 9, WFA2 mixed in a ratio of control and bran:fishmeal:agarose=9:1:1 (w/w) was 20.0% and 23.3%, respectively, whereas It was confirmed that WFA1 had the highest mortality rate at 46.7%, followed by WFA3 (43.3%) and WFA4 (33.3%), which was 3.3-26.7% higher than that of the control group.

상기 결과를 통해, 아메리카왕거저리의 인공 사료로서는 밀기울:어분:아가로스=9:1:1(w/w) 비율로 혼합한 WFA2가 가장 적합함을 확인하고, 하기 표 5와 같이 본원 발명에 따른 아메리카왕거저리의 인공 사료 조성물(WFA2)의 구체적인 조성비를 확립하였다.Through the above results, it was confirmed that WFA2 mixed in a ratio of bran:fishmeal:agarose=9:1:1 (w/w) is most suitable as an artificial feed for the American king gooseberry, and according to the present invention as shown in Table 5 below. The specific composition ratio of the artificial feed composition (WFA2) of American king geese was established.

WFA2(본원 발명에 따른 아메리카왕거저리의 인공 사료 조성물)WFA2 (Artificial feed composition of American king geese according to the present invention) 밀기울(g)Bran (g) 9090 어분(g)Fish meal (g) 1010 아가로스(g)Agarose (g) 1010 소르빈산(g)Sorbic acid (g) 0.20.2 메틸파라벤(g)Methylparaben (g) 0.20.2 식용유(ml)Cooking oil (ml) 22 종합비타민(g)Multivitamin (g) 1010 DW(ml)DW(ml) 400400

실시예 4. 본원 발명에 따른 아메리카왕거저리의 인공 사료 조성물의 발육특성 및 사육효율 상세 비교Example 4. Detailed comparison of growth characteristics and breeding efficiency of the artificial feed composition of American king geese according to the present invention

4-1. 발육특성 비교4-1. Comparison of development characteristics

상기 실시예 3에서 제조한 본원 발명에 따른 아메리카왕거저리의 인공 사료 조성물(밀기울:어분:아가로스=9:1:1(w/w), WFA2)과 밀기울만을 포함하는 대조군의 발육특성을 확인하였다. 구체적으로, 상기 실시예 3에서 제조한 인공 사료 조성물과 동량의 밀기울(밀기울 100%)을 아메리카왕거저리 유충에 급이하여 령별 발육기간, 령별 체중 및 폐사율을 측정하였다. 사육실 환경조건은 30℃, 습도 65%, 광주기 9L/15D, 조도 1800lux로 설정하였으며, 아메리카왕거저리 유충을 40마리씩 2구를 설정(총 80마리)하여 3반복으로 실험을 수행하였다. 그 결과를 도 10 내지 12에 나타내었다.The artificial feed composition (wheat bran: fish meal: agarose = 9: 1: 1 (w/w), WFA2) prepared in Example 3 and the control group containing only bran was confirmed. I did. Specifically, the same amount of bran (wheat bran 100%) of the artificial feed composition prepared in Example 3 was fed to the larvae to measure the growth period by age, body weight by age, and mortality. The environmental conditions of the breeding room were set at 30°C, humidity 65%, photoperiod 9L/15D, and illumination 1800lux, and the experiment was performed in 3 repetitions by setting 2 heads of 40 larvae each (total 80). The results are shown in FIGS. 10 to 12.

도 10에 나타낸 바와 같이, 2종 인공 사료의 1-15령까지 발육기간은 본원 발명에 따른 인공 사료가 86.6±6.5일로 대조군인 밀기울(92.1±6.4일)보다 5.5일이나 단축됨을 확인하였다(P=0.109).As shown in FIG. 10, it was confirmed that the growth period of the two types of artificial feeds up to 1-15 years of age was shortened by 5.5 days from the control group bran (92.1±6.4 days) to 86.6±6.5 days for the artificial feed according to the present invention (P). =0.109).

체중의 경우, 도 11에 나타낸 바와 같이, 5령까지는 거의 차이가 없다가 6령 이후부터 인공 사료 급이군에서 체중이 증가함을 확인하였다. 특히, 10령부터는 2배 정도 증가하고 전용시기가 가까워질수록 체중이 평균 1.2-1.7배 증가함을 확인하였다.In the case of body weight, as shown in FIG. 11, there was almost no difference until age 5, but it was confirmed that the body weight increased in the artificial feed-fed group after age 6. In particular, it was confirmed that the weight increased by about 2 times from the age of 10, and the average weight increased by 1.2-1.7 times as the transfer period became closer.

한편, 12령까지 아메리카왕거저리 유충의 폐사율을 비교 조사한 결과, 도12에 나타낸 바와 같이, 본 발명에 따른 인공 사료와 대조군의 폐사율은 18.75%로 유의한 차이가 없음을 확인하였다.On the other hand, as a result of comparing and investigating the mortality rate of larvae of the American king geese up to age 12, it was confirmed that there was no significant difference between the mortality rate of the artificial feed according to the present invention and the control group as 18.75%, as shown in FIG.

4-2. 전용시기, 우화율 비교4-2. Exclusive period, fable rate comparison

본원 발명에 따른 인공 사료 조성물 급이 시의 아메리카왕거저리의 발육단계별 전용시기를 확인하고, 그 결과를 도 13 및 도 14에 나타내었다. When feeding the artificial feed composition according to the present invention, the timing of exclusive use for each stage of development of the American king geeseoli was confirmed, and the results are shown in FIGS. 13 and 14.

도 13에 나타낸 바와 같이, 인공 사료의 경우 12~13령부터 전용시기인데 반해 대조군인 밀기울은 14령부터 전용시기가 시작되어 인공사료보다 발육이 늦어지는 경향을 보임을 확인하였다. 사육시작 105일을 기점으로, 인공 사료 급이한 실험군 중 전용시기에 있는 유충은 83.8%로, 이는 대조군인 밀기울(59.5%)보다 24.3%나 많은 수치임을 확인하였다(P=0.020). As shown in FIG. 13, in the case of artificial feed, it was confirmed that the transfer period was started from the age of 12 to 13, whereas the transfer period of bran, which was the control group, started from the age of 14, so that the growth was more delayed than the artificial feed. Starting from the 105th day of breeding, the number of larvae in the dedicated period among the experimental groups fed with artificial feed was 83.8%, which was 24.3% higher than that of the control bran (59.5%) (P=0.020).

또한, 도 14에 나타낸 바와 같이, 인공 사료의 전용기간(17.2±4.0일)과 1령에서 성충까지의 기간(17.2±4.0일)은 밀기울의 전용기간보다는 1.2일 길고, 성충기간보다는 약 4일 단축됨을 확인하였다. In addition, as shown in Fig. 14, the conversion period of artificial feed (17.2±4.0 days) and the period from 1 year old to adult (17.2±4.0 days) are 1.2 days longer than the conversion period of bran, and about 4 days than the adult insect period. It was confirmed that it was shortened.

아울러, 도 15에 나타낸 바와 같이, 성충이 되는 우화율 역시 인공 사료가 30%로 대조군인 밀기울보다 20%나 증가함을 확인하였다(P=0.0001).In addition, as shown in FIG. 15, it was confirmed that the rate of emergence of becoming an adult was also increased by 20% in the artificial feed to 30%, compared to the control bran (P=0.0001).

4-3. 사육효율 비교4-3. Comparison of breeding efficiency

본원 발명에 따른 인공 사료 조성물의 먹이 효율성을 확인하였다. 구체적으로, 상기 실시예 3에서 제조한 인공 사료 조성물과 동량의 밀기울(밀기울 100%)을 아메리카왕거저리 유충에 급이하여 령별 체중, 령별 먹이 소모량(인공 사료의 경우 수분 증발량 적용) 및 발육단계에 따른 사료 효율(ECD, 체중 증가량/사료 섭취량)을 측정하였다. 사육실 환경조건은 30℃, 습도 65%, 광주기 9L/15D, 조도 1800lux로 설정하였으며, 아메리카왕거저리 유충을 10마리씩 2구를 설정(총 20마리)하여 3반복으로 실험을 수행하였다. 그 결과를 도 16에 나타내었다. It was confirmed the feeding efficiency of the artificial feed composition according to the present invention. Specifically, the same amount of bran (wheat bran 100%) of the artificial feed composition prepared in Example 3 was fed to the larvae of larvae, and the weight by age, the amount of food consumed by age (in the case of artificial feed, water evaporation amount was applied) and the development stage. According to the feed efficiency (ECD, weight gain / feed intake) was measured. The environmental conditions of the breeding room were set at 30°C, humidity 65%, photoperiod 9L/15D, and illumination 1800lux, and the experiment was performed in 3 repetitions by setting 2 heads (total 20) of 10 larvae. The results are shown in FIG. 16.

도 16에 나타낸 바와 같이, 1마리당 체중을 조사한 결과, 인공 사료 급이군이 7령 이후부터 밀기울보다 거의 2배 이상 체중이 증가함을 확인하였다. 또한, 1마리당 먹이소모량은 인공 사료 급이군이 8령까지 많다가 9령 이후부터는 대조군에서 증가함을 확인하였다.As shown in Fig. 16, as a result of examining the body weight per animal, it was confirmed that the artificial feed-fed group increased the body weight by almost two times or more than the bran from 7 years old. In addition, it was confirmed that the amount of food consumed per animal increased in the control group after the age of 9 after the artificial feed-fed group increased to 8 years old.

사료 효율의 경우, 도 17에 나타낸 바와 같이, 8령 이후부터 인공 사료 급이군의 사료 효율이 밀기울보다 현저히 증가함을 확인하였다.In the case of feed efficiency, as shown in FIG. 17, it was confirmed that the feed efficiency of the artificial feed feeding group increased significantly from the age of 8 to that of bran.

상기 결과를 통해, 아메리카왕거저리 사육용 인공 사료 조성물은 밀기울:어분:아가로스=9:1:0.5 내지 2(w/w)의 비율로 혼합하는 경우, 바람직하게는 밀기울:어분:아가로스=9:1:1 내지 2(w/w)의 비율로 혼합하는 경우, 보다 바람직하게는 밀기울:어분:아가로스=9:1:1 내지 1.5(w/w)의 비율로 혼합하는 경우, 보다 더 바람직하게는 밀기울:어분:아가로스=9:1:1 내지 1.25(w/w)의 비율로 혼합하는 경우, 가장 바람직하게는 밀기울:어분:아가로스=9:1:1(w/w)의 비율로 혼합하는 경우 아메리카왕거저리 유충의 발육효율이 가장 우수하고 사육효율도 가장 높음을 확인하였다.Through the above results, when mixing the artificial feed composition for breeding bran: fish meal: agarose = 9: 1: 0.5 to 2 (w / w), preferably bran: fish meal: agarose = When mixing in a ratio of 9:1:1 to 2 (w/w), more preferably bran:fishmeal:agarose=9:1:1 to 1.5 (w/w), more More preferably, when mixing in a ratio of bran:fishmeal:agarose=9:1:1 to 1.25 (w/w), most preferably bran:fishmeal:agarose=9:1:1 (w/w) When mixed at the ratio of ), it was confirmed that the growth efficiency of the larva was the best and the breeding efficiency was the highest.

본 발명은 밀기울 대체먹이원으로 어분을 사용하여 생산비 절감효과를 얻을 수 있을 뿐만 아니라, 아가로스를 혼합함에 따라 유용곤충 사육과정에서 문제되는 분진을 효율적으로 제거할 수 있고, 아메리카왕거저리에게 급이가 용이한 제형의 인공 사료 조성물을 제공한다는 특징이 있다. 나아가, 사육환경이 개선됨과 동시에 높은 발육효율 및 낮은 폐사율을 달성할 수 있어, 주요 곤충자원인 아메리카왕거저리의 대량사육 원천기술 확보 및 그에 따른 농가의 소득향상이 기대된다.The present invention not only can obtain the effect of reducing production cost by using fishmeal as an alternative feeding source for bran, but also can efficiently remove the dust problem in the process of breeding useful insects by mixing agarose, and feeding It is characterized in that it provides an artificial feed composition in an easy formulation. Furthermore, it is possible to achieve high growth efficiency and low mortality rate while improving the breeding environment, so it is expected to secure source technology for mass rearing of the major insect resource, the American king geese, and to increase the income of farm households accordingly.

이상으로 본 발명의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현 예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항과 그의 등가물에 의하여 정의된다고 할 것이다.As the specific parts of the present invention have been described in detail above, it is obvious that these specific techniques are only preferred embodiments for those of ordinary skill in the art, and thus the scope of the present invention is not limited thereto. Therefore, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (7)

밀기울, 어분 및 아가로스를 포함하는 아메리카왕거저리 사육용 인공 사료 조성물.
An artificial feed composition for rearing red snapper, comprising bran, fish meal and agarose.
제1항에 있어서, 상기 밀기울, 어분 및 아가로스는 9:1:0.5 내지 2(w/w) 비율로 혼합되는 것을 특징으로 하는, 조성물.
The composition of claim 1, wherein the bran, fish meal and agarose are mixed in a ratio of 9:1:0.5 to 2 (w/w).
제2항에 있어서, 상기 밀기울, 어분 및 아가로스는 9:1:1 내지 1.5(w/w) 비율로 혼합되는 것을 특징으로 하는, 조성물.
The composition of claim 2, wherein the bran, fish meal and agarose are mixed in a ratio of 9:1:1 to 1.5 (w/w).
제1항에 있어서, 상기 조성물은 소르빈산, 메틸파라벤, 식용유, 종합비타민 및 증류수로 이루어진 군에서 선택된 하나 이상을 더 포함하는 것을 특징으로 하는, 조성물.
The composition of claim 1, wherein the composition further comprises at least one selected from the group consisting of sorbic acid, methylparaben, cooking oil, multivitamin, and distilled water.
밀기울, 어분 및 아가로스를 9:1:0.5 내지 2(w/w)로 혼합하는 단계;를 포함하는 아메리카왕거저리 사육용 인공 사료 조성물의 제조 방법.
Mixing bran, fish meal, and agarose in a ratio of 9:1:0.5 to 2 (w/w); method for producing an artificial feed composition for breeding red snapper.
제1항 내지 제3항 중 어느 하나의 조성물을 아메리카왕거저리에 급이하여 아메리카왕거저리를 사육하는 방법.
A method for rearing American king geese by feeding the composition of any one of claims 1 to 3.
제6항에 있어서, 상기 사육은 온도 25 내지 33℃, 습도 60 내지 70%, 광주기 9L/15D 및 조도 1800lux의 사육조건 하에서 이루어지는 것을 특징으로 하는, 방법. The method of claim 6, wherein the breeding is carried out under breeding conditions of a temperature of 25 to 33°C, a humidity of 60 to 70%, a photoperiod of 9L/15D, and an illuminance of 1800 lux.
KR1020190095115A 2019-08-05 2019-08-05 Artificial Diet Composition for Zophobas atratus KR102292714B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020190095115A KR102292714B1 (en) 2019-08-05 2019-08-05 Artificial Diet Composition for Zophobas atratus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020190095115A KR102292714B1 (en) 2019-08-05 2019-08-05 Artificial Diet Composition for Zophobas atratus

Publications (2)

Publication Number Publication Date
KR20210016842A true KR20210016842A (en) 2021-02-17
KR102292714B1 KR102292714B1 (en) 2021-08-23

Family

ID=74732446

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020190095115A KR102292714B1 (en) 2019-08-05 2019-08-05 Artificial Diet Composition for Zophobas atratus

Country Status (1)

Country Link
KR (1) KR102292714B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220159766A (en) * 2021-05-26 2022-12-05 전라북도(농업기술원) Breeding Artificial Diet Composition for breeding Gampsocleis ussuriensis And Artificial Breeding Method of Gampsocleis ussuriensis

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002518031A (en) * 1998-06-23 2002-06-25 ユニバーシティ オブ メリーランド Use of oils with high lauric acid content as animal feed
US8647686B1 (en) * 2007-08-30 2014-02-11 The United States Of America, As Represented By The Secretary Of Agriculture Insect diet formulations and methods for rearing insects
KR20140122839A (en) * 2013-04-11 2014-10-21 대한민국(농촌진흥청장) Jelly type composition of supply water for Tenebrionidae larvae and manufacturing method thereof
CN104256141A (en) * 2014-09-18 2015-01-07 合肥市好旺养殖科技有限公司 Feed for yellow meal worms and preparation method thereof
KR20150001806U (en) 2013-11-01 2015-05-12 농업회사법인 (주)한국유용곤충연구소 Breeding Kit for Education and artificial diet of mealworm
KR20170052128A (en) * 2015-11-04 2017-05-12 대한민국(농촌진흥청장) Diet composition for Tenebrionidae larvae
KR20170055885A (en) 2015-11-12 2017-05-22 최영숙 The feeding composition for imago of Tenebrio molitor and manufacturiing method of it
KR20170057594A (en) * 2015-11-17 2017-05-25 주식회사 에스티에스연구소 Artificial feed for luciola laterlais
KR20170131913A (en) * 2016-05-23 2017-12-01 대한민국(농촌진흥청장) Feed composition comprising Tenebrio molitor for breeding Scolopendra subspinipes or artificial breeding method for mass production of Scolopendra subspinipes using thereof
KR20190051322A (en) * 2017-11-06 2019-05-15 대한민국(농촌진흥청장) Method for artificial incubation of Zophobas atratus Fabricius
KR20190052251A (en) * 2017-11-08 2019-05-16 대한민국(농촌진흥청장) Breeding Artificial Diet composition for breeding Locusta migratoria or Artificial breeding method of Locusta migratoria

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002518031A (en) * 1998-06-23 2002-06-25 ユニバーシティ オブ メリーランド Use of oils with high lauric acid content as animal feed
US8647686B1 (en) * 2007-08-30 2014-02-11 The United States Of America, As Represented By The Secretary Of Agriculture Insect diet formulations and methods for rearing insects
KR20140122839A (en) * 2013-04-11 2014-10-21 대한민국(농촌진흥청장) Jelly type composition of supply water for Tenebrionidae larvae and manufacturing method thereof
KR20150001806U (en) 2013-11-01 2015-05-12 농업회사법인 (주)한국유용곤충연구소 Breeding Kit for Education and artificial diet of mealworm
CN104256141A (en) * 2014-09-18 2015-01-07 合肥市好旺养殖科技有限公司 Feed for yellow meal worms and preparation method thereof
KR20170052128A (en) * 2015-11-04 2017-05-12 대한민국(농촌진흥청장) Diet composition for Tenebrionidae larvae
KR20170055885A (en) 2015-11-12 2017-05-22 최영숙 The feeding composition for imago of Tenebrio molitor and manufacturiing method of it
KR20170057594A (en) * 2015-11-17 2017-05-25 주식회사 에스티에스연구소 Artificial feed for luciola laterlais
KR20170131913A (en) * 2016-05-23 2017-12-01 대한민국(농촌진흥청장) Feed composition comprising Tenebrio molitor for breeding Scolopendra subspinipes or artificial breeding method for mass production of Scolopendra subspinipes using thereof
KR20190051322A (en) * 2017-11-06 2019-05-15 대한민국(농촌진흥청장) Method for artificial incubation of Zophobas atratus Fabricius
KR20190052251A (en) * 2017-11-08 2019-05-16 대한민국(농촌진흥청장) Breeding Artificial Diet composition for breeding Locusta migratoria or Artificial breeding method of Locusta migratoria

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
농사로 영농활용기술 2017* *
한국곤충학회 추계 학술발표 2017* *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220159766A (en) * 2021-05-26 2022-12-05 전라북도(농업기술원) Breeding Artificial Diet Composition for breeding Gampsocleis ussuriensis And Artificial Breeding Method of Gampsocleis ussuriensis

Also Published As

Publication number Publication date
KR102292714B1 (en) 2021-08-23

Similar Documents

Publication Publication Date Title
CN107136300A (en) A kind of jerusalem artichoke animal and fowl fodder and preparation method thereof
CN104012792A (en) Silkworm excrement-containing feed for chicken and preparation method thereof
Mukhopadhyay et al. Applied nutrition in freshwater prawn, Macrobrachium rosenbergii, culture
CN103494019A (en) Fermented feed special for broiler chickens
KR20190052251A (en) Breeding Artificial Diet composition for breeding Locusta migratoria or Artificial breeding method of Locusta migratoria
CN109497275A (en) A kind of feed improving laying hen quality of egg
Adama et al. Feed intake, growth Performance and nutrient digestibility of broiler chicks fed Diets containing varying levels of sorghum dried brewers Grains
KR102292714B1 (en) Artificial Diet Composition for Zophobas atratus
CN107114324B (en) Method for breeding quasipaa spinosa by using vitamin-rich yellow mealworms
CN107348214A (en) A kind of feed for promoting goose growth containing edible fungus bran
Byers Systems of beef cattle feeding and management to regulate composition of growth to produce beef carcasses of desired composition
Atapattu et al. Effects of gradual feed dilution with inert or less nutritive materials on growth performance, feed cost and meat organoleptic properties of broiler chicken
Hassan et al. Studies on growth, organs weight and haematological parameters of broiler chicken fed graded level of sun dried cassava root meal
CN108567080A (en) A kind of Gairino maschato feed and its application method
Minh et al. Effect of scavenging and protein supplement on the feed intake and performance of improved pullets and laying hens in northern Vietnam
Duyet et al. Effect of high dietary levels of sweet potato leaves on the reproductive performance of pure and crossbred Mong Cai sows
Oluwatosin et al. Growth performance of the African Catfish (Clarias Gariepinus) fed soaked ripe plantain peel meal (Musa Paradisiaca)
Ladokun et al. Sweet potato as a feed resource for layer production in Nigeria.
CN113397063A (en) Compound feed for young laying hens and preparation method thereof
Shaahu et al. Effect of replacing maize with graded levels of sweet potato vine–cassava composite meal on growth performance, nutrient digestibility, carcass characteristics and economics of production of weaned rabbits
Oyetunji et al. Growth efficiency and profitability indices of African catfish (Clarias gariepinus) fingerlings fed with different levels of Adansonia digitata (Baobab) seed meal
Babalola et al. Effects of dietary wilted water leaf meal on the growth performance of Oreochromis niloticus fingerlings.
Shaahu et al. Effect of replacing maize with composite cassava meal (CCM) on growth and economics performance of rabbit
CN108308465A (en) A kind of bionical feed and preparation method thereof of cultivation spider
Oyewole et al. Productive and haematological performance of growing pullets fed fermented sweet orange (Citrus sinensis) fruit peel meal based diets.

Legal Events

Date Code Title Description
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant