KR20220159766A - Breeding Artificial Diet Composition for breeding Gampsocleis ussuriensis And Artificial Breeding Method of Gampsocleis ussuriensis - Google Patents

Abstract

The present invention relates to an artificial feed composition for breeding Gampsocleis ussuriensis and an artificial breeding method of Gampsocleis ussuriensis using the same. The artificial feed composition for breeding Gampsocleis ussuriensis according to the present invention is easier to be supplied than conventional vegetable or animal natural feeds, and can be usefully used for easily breeding Gampsocleis ussuriensis for home and exhibition use since there is no difference in eating amount and growth characteristics.

Description

Artificial feed composition for breeding katydids and artificial breeding method of katydids using the same {Breeding Artificial Diet Composition for breeding Gampsocleis ussuriensis And Artificial Breeding Method of Gampsocleis ussuriensis}

The present invention relates to an artificial feed composition for breeding long-winged katydids and an artificial breeding method for long-winged katydids using the same.

Emotional insects are insects used in theme parks, experience farms, etc., including pets and insects for experiential learning, and are used in the 6th industry by activating human cognition, emotions, and physical functions. Recently, interest in emotional insects and healing agriculture is increasing as the size of the companion animal market has expanded due to population aging, expansion of single-person households, and increased leisure activities, and the emotional healing effect using insects has been identified.

However, despite the high possibility of continuous development of the emotional insect market, only about 50 types of insects have been developed, and mass production technology is lacking. There are only about 10 species, including flower radishes, and there is a limit to growth due to lack of product diversity.

On the other hand, katydid is a representative sound insect, and it is an insect that helps to cultivate human emotions to the extent that there has been a custom of listening to the sound of crying in the summer by raising it in a house made of barley stalks in Korea since ancient times. In China, katydids are also used for food, and fighting katydids are an important folk game in China, and groups such as the katydid association and the katydid club have been established for the purpose of research, development, use, admiration, and entertainment of katydids. Back gynecological insects are positioned as products for relaxation/leisure.

However, despite the increase in demand for katydids, there is no report on the detailed ecology of katydids insects and mass breeding techniques for year-round production, and there is no research on commercialization other than katydids for listening to sounds. As for the current status of domestic research, taxonomic reviews of Korean katydids and studies on the life history of katydids have been conducted, but studies on the ecological characteristics and mass breeding techniques of katydids are lacking.

Therefore, it is necessary to develop breeding techniques for gynecological insects that farmers and consumers want, and to expand the method of grafting insects by establishing a support system for emotional insect education farms and in connection with departments related to psychological healing.

Under this background, the present inventors have made intensive research efforts to develop an artificial feed for mass-breeding long-winged katydids, one of the katydid insects, and as a result, breeding katydids using artificial feeds containing animal and vegetable food sources. The present invention was completed by confirming that the feeding was easy and the development was good compared to biological feed.

Republic of Korea Patent Publication No. 10-2020-0072129

The present invention aims to solve the above problems and other problems related thereto.

One exemplary object of the present invention is to breed a long-winged katydid (Gampsocleis ussuriensis) containing a vegetable food source, an animal food source, pyridoxine, calcium pantotenate and crotonic acid. It is to provide an artificial feed composition for use.

Another exemplary object of the present invention is to provide a method for producing an artificial feed for long-winged katydids comprising the following steps.

(a) mixing a vegetable food source, an animal food source, pyridoxine, calcium pantotenate, and crotonic acid;

(b) sterilizing the mixture; and

(c) removing air bubbles from the sterilized mixture and then refrigerating to prepare an artificial feed composition for breeding katydid.

Another exemplary object of the present invention is to provide an artificial breeding method for long-winged katydids, characterized in that the artificial feed composition is fed to the long-winged katydids for breeding.

The technical problem to be achieved according to the technical idea of the invention disclosed in this specification is not limited to the problem to solve the problems mentioned above, and another problem not mentioned can be clearly understood by those skilled in the art from the following description. There will be.

A detailed description of this is as follows. Meanwhile, each description and embodiment disclosed in this application may also be applied to each other description and embodiment. That is, all combinations of various elements disclosed in this application fall within the scope of this application. In addition, the scope of the present application is not to be construed as being limited by the specific descriptions described below.

As one aspect for achieving the above object, the present invention provides a vegetable food source, an animal food source, pyridoxine, calcium pantothenate (Ca-pantotenate) and crotonic acid (Crotonic acid), including a long-winged katydid It provides an artificial feed composition for breeding.

The term "long-winged katydid" of the present invention is an insect of the genus Cactus katydid, which is similar to the katydid of the same genus but has a smaller body shape and lives mainly in bushes, grasslands, farmlands, riversides, embankments, and mountain valleys. It is distributed throughout the Korean Peninsula, and is known to be distributed in Hokkaido, Japan, Northeast China, and Far East Russia.

In the present invention, the artificial feed composition for breeding long-winged katydid refers to a feed composition that can exhibit growth characteristics beyond natural feed when fed by mixing various necessary nutrients of long-winged katydid as artificially prepared food.

In the present invention, the animal food source may be mealworms or crickets, specifically mealworms, but is not limited thereto. The animal food source of the present invention may be dried mealworms or crickets or dried powdered powder, but is not limited thereto.

In the present invention, the vegetable food source may be any one selected from cabbage, Chinese cabbage, corn, and kale, and may be specifically cabbage, but is not limited thereto.

In the present invention, the plant food source may be a seedling grown for 20 to 30 days by pot-sowing the seeds of the plants.

In the present invention, the vegetable food source and the animal food source may be mixed in a weight ratio of 9:1 to 1:9, for example, may be mixed in a weight ratio of 2:8 to 4:6, for example It may be mixed in a weight ratio of 3:7, but is not limited thereto.

In the present invention, the pyridoxine serves to enhance amino acid metabolism by providing vitamin B6, the calcium pantothenate is a composition for tissue growth by fortifying calcium salts, and the crotonic acid is an unsaturated fatty acid.

In the present invention, the pyridoxine and calcium pantothenate may be mixed in a weight ratio of 30: 1 to 5: 1, and the calcium pantothenate and crotonic acid are mixed in a weight ratio of 1: 50 to 1: 15 The pyridoxine and crotonic acid may be mixed in a weight ratio of 1:5 to 1:1.

In the present invention, the composition is an artificial feed composition for long-winged katydid, sorbic acid, gallic acid, corn starch, ascorbic acid, citric acid, carrageenan, cellulose, It may further include Soybean meal, Agar and water.

In the present invention, the sorbic acid serves as a nutrient, and may be added to inhibit microbial growth and prevent spoilage in artificial feed. The gallic acid is a reducing agent for improving cholesterol, the corn starch is used as a kind of starch that imparts viscosity or adhesiveness to artificial feed, and the ascorbic acid acts as an antioxidant as vitamin C and replenishes vitamins of the long-winged katydid. For this, the citric acid is a composition that promotes calcium absorption by acting as a preservative for artificial feed. The carrageenan is a composition that serves as a stabilizer added to give adhesiveness and gelation to the artificial feed, and the cellulose serves as a digestive agent when the long-winged katydid consumes the artificial feed. The soybean meal is a protein source, and the agar serves as a gelling agent for artificial feed.

In the present invention, the sorbic acid and gallic acid may be mixed in a weight ratio of 1: 3 to 1: 2, and the ascorbic acid and citric acid may be mixed in a weight ratio of 5: 12 to 7: 10, and the Carrageenan and cellulose may be mixed in a weight ratio of 2:1 to 4:1, and the soybean meal and agar may be mixed in a weight ratio of 20:1 to 25:1.

In the present invention, the artificial feed composition for breeding katydid may be divided into individual groups according to the purpose and mixed after preparation. group, a second group containing crotonic acid, ascorbic acid, and citric acid as preservatives, a third group containing carrageenan and cellulose as an adhesive, and a fourth group containing soybean meal as a protein source. can be

In the present invention, the artificial feed composition for breeding katydid may further include at least one of fish meal, mulberry meal, raspberry meal, and blueberry meal as an additive. These additives are intended to increase the nutritional and functional components of artificial feed, and the growth characteristics of long-winged katydids can be improved due to the addition of additives.

The additive and the artificial feed composition may be mixed in a weight ratio of 3:5 to 9, but is not limited thereto.

The additive may be mixed with the artificial feed composition or supplied separately from the artificial feed composition.

Specifically, in the experimental example of the present invention, when both vegetable food sources and animal food sources were included in the artificial feed for long-winged katydids, the development period was shorter and the growth was smoother than in other cases, and the mixing ratio of vegetable and animal food sources was high. At 3:7, it was confirmed that the total intake was the highest and the development period was short, and when additives were added to the artificial feed, it was confirmed that the development period was greatly reduced compared to the control group.

Based on these test examples, it was confirmed that the artificial feed composition for breeding katydids of the present invention can be usefully used for breeding katydids.

As another aspect of the present invention, the present invention provides a method for producing an artificial feed composition for longwing katydids comprising the following steps.

(a) mixing a vegetable food source, an animal food source, pyridoxine, calcium pantotenate, and crotonic acid;

(b) sterilizing the mixture; and

(c) removing air bubbles from the sterilized mixture and then refrigerating to prepare an artificial feed composition for breeding katydid.

In the present invention, the manufacturing method includes sorbic acid, gallic acid, corn starch, ascorbic acid, citric acid, carrageenan, cellulose, soybean meal ( Soybean meal), agar and water may be further included and mixed.

As another aspect of the present invention, the present invention provides an artificial breeding method for long-winged katydids, characterized in that the artificial feed composition is fed to and reared.

The artificial feed composition for breeding long-winged katydids according to the present invention is easy to feed compared to conventional vegetable or animal natural feeds, and there is no difference in eating amount and growth characteristics, so it can be easily used for breeding katydids for home and exhibition use. .

1 shows an artificial feed composition for long-winged katydid prepared according to the present invention.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1: Preparation of artificial feed composition according to the present invention

1-1. food source selection

For the selection of food sources for artificial feed for breeding katydid, growth characteristics were compared between the case of providing a vegetable food source alone and the case of providing a mixture of a vegetable food source and an animal food source. Arrowroot, cucumber, radish, cabbage, Chinese cabbage, kale, and corn were used as plant food sources, and mealworms were used as animal food sources. The plant food source was provided by sowing each plant seed in a pot and putting the seedlings for about 20 to 30 days in a pot breeding container. Long-winged katydids were placed in a 20 x 20 x 20 cm breeding box and each food source was supplied to compare growth characteristics.

As a result, as shown in Table 1, when only vegetable food sources were supplied, the development period was longer and the parasite rate was lower than when both vegetable and animal food sources were supplied. It was found that the parasite rate was relatively high. In the case of radish, the growth rate was the same as that of cabbage and Chinese cabbage, but the development period was very long.

food source Nymph development period (day) Insecticide rate (%) vegetable animal nature 1st 2nd 3rd 4th 5th total arrowroot - 10 17.6 9.8 8 - 45.4 0 cucumber - 12 20 12.8 19 - 63.8 20 cabbage - 11 16 12 13.8 12 64.8 60 radish - 11.5 20.6 11.6 18.2 12.3 74.2 60 napa cabbage - 12 18 10.5 13.3 12.7 66.5 60 napa cabbage Mealworm 12 14 10.5 13.3 12.7 49.8 100 cabbage Mealworm 11 16 12 11.8 12 50.8 80 Kale Mealworm 11.2 13.2 12.5 11 12.5 47.9 80 corn Mealworm 11.5 13.5 11.2 10.5 12.3 46.7 80

In addition, the weight of katydid nymphs when a vegetable food source or animal food source is provided alone (Table 2), and the weight of katydid nymphs and adults when a mixture of animal and vegetable food sources is provided ( Table 3) was compared.

As a result, as shown in Tables 2 and 3, when only vegetable food sources were supplied, in the case of arrowroot and cucumber, long-winged katydids in the 5th instar could not be identified due to stunted growth, and cabbage, radish and Chinese cabbage were also supplied with mealworms. It was confirmed that the weight decreased compared to the time when the growth was sluggish.

When only mealworms, which are animal food sources, were supplied, the weight of nymphs decreased compared to when they were supplied together with vegetable food sources, and it was confirmed that their growth was sluggish. In addition, when animal food sources and plant food sources are supplied together, it was found that good growth is possible in all of cabbage, cabbage, kale and corn when compared according to the type of vegetable food source. As a result, it was confirmed that the development of long-winged katydids was excellent when the vegetable food source or the animal food source was supplied together rather than provided alone.

food source nymph weight (g) vegetable animal nature 1st 2nd 3rd 4th 5th arrowroot - 0.17 0.33 0.37 0.42 - cucumber - 0.17 0.23 0.3 0.46 - cabbage - 0.18 0.31 0.55 0.8 1.62 radish - 0.18 0.28 0.4 0.56 1.56 napa cabbage - 0.16 0.3 0.45 0.79 1.55 - Mealworm 0.16 0.31 0.65 0.89 1.86

food source nymph weight (g) Adult weight (g) vegetable animal nature 1st 2nd 3rd 4th 5th cancer number napa cabbage Mealworm weight (g) 0.6 0.6 0.8 2.1 2.2 2.8 3 cabbage Mealworm weight (g) 0.6 0.6 1.5 1.8 2.5 3 2.4 Kale Mealworm weight (g) 0.9 1.3 1.7 2.4 2.1 2.7 2.5 corn Mealworm weight (g) 0.4 0.7 2.1 2.2 2.5 2.4 2

1-2. artificial feed manufacturing

In order to prepare an artificial feed for breeding katydid, the feed composition shown in Table 4 below, including a vegetable food source and an animal food source, was prepared with reference to the results of 1-1 above. Plant food sources and animal food sources were prepared in a ratio (weight ratio) of 9:1, 7:3, 5:5, 3:7, and 1:9, respectively.

The first group, which includes sorbic acid, pyridoxine, calcium pantothenate, and gallic acid, serves as a nutrient for long-winged katydids, and the second group, which includes crotonic acid, ascorbic acid, and citric acid, serves as a preservative for artificial feed. The third group, including carrageenan and cellulose, serves as an adhesive so that the composition can be well mixed with each other, and the fourth group, including soybean meal, is a protein source. Corn starch and agar were added to maintain viscosity during kneading.

group feed composition weight (g) One sorbic acid One pyridoxine 0.6 Calcium pentotenate (Ca-pantotenate) 0.05 Gallic acid 2.3 corn starch 30 2 crotonic acid 2 ascorbic acid 7.5 citric acid 9.1 corn starch 50 3 carrageenan 30 cellulose 10 4 soybean meal 235 Plant-to-animal ratio 9:1 7:3 5:5 3:7 1:9 Plant food sources (cabbage, Chinese cabbage, corn, or kale) 144 112 80 48 16 Animal food sources (mealworms) 16 48 80 112 144 Agar 10 5 water 700mL

Groups 1, 2, 3, and 4 were each prepared, mixed with water, kneaded in a blender, and then sterilized at 120° C. for 20 minutes. Thereafter, air bubbles were removed, cooled on a clean bench, and stored in a refrigerator. As a result, as shown in FIG. 1, an artificial feed for long-winged katydid was prepared.

Experimental Example 1: Confirmation of developmental characteristics of long-winged katydid by mixing ratio

1-1. Comparison of nymph feeding

Growth characteristics were confirmed by feeding artificial feeds with different mixing ratios to long-winged katydids.

Specifically, after feeding artificial feed in a container at 3-5 g for 1 to 3 instars and 5 to 10 g for 4 to 5 instars of long-winged katydid, the intake amount was checked while replacing it at 2-3 day intervals.

As a result of analyzing the intake of nymphs by varying the mixing ratio of plant food source and animal food source of artificial feed, as shown in Table 5, cabbage as a vegetable food source at the 5th instar: Feeding amount when the ratio of mealworm as an animal food source is 3:7 The average daily intake was 12.5 g, the highest at 1.8 g, and the total intake was the highest at 3:7, at 23.7 g.

mixing ratio
(Plants: Animals) Amount eaten (g) Average
1 day
food intake
(g) 1st 2nd 3rd 4th 5th total T M T M T M T M T M T M 9:1 2.0 0.7 2.6 0.7 2.9 0.7 4.1 0.8 11.6 1.7 23.1 1.0 1.0 7:3 1.8 0.6 2.7 0.7 2.9 0.7 4.0 0.8 10.7 1.5 22.1 0.9 0.9 5:5 2.1 0.7 2.5 0.6 2.7 0.7 3.8 0.8 9.4 1.6 22.3 0.9 0.9 3:7 2.1 0.7 2.3 0.6 2.5 0.6 4.2 0.8 12.5 1.8 23.7 1.0 1.0 1:9 2.2 0.7 2.1 0.5 2.3 0.6 3.1 0.6 6.1 1.5 21.6 0.8 0.8

T: total intake, M: daily average intake

1-2. Comparison of nymph development period and reproductive rate

The growth period of nymphs and the rate of larvae were analyzed by varying the mixing ratio of plant food source and animal food source of artificial feed. As a control, cabbage and mealworms, which are living organisms of animals and plants, were fed and compared. As a result, as shown in Table 6, when artificial feed with a vegetable food source: animal food source ratio of 3:7 was fed, the parasite rate was 100%, and the development period of nymphs was 36.7 days, the highest compared to other mixing ratios. It was confirmed that the long-winged katydid rapidly grew by appearing short.

mixing ratio
(Plants: Animals) Nymph development period (day) reproductive rate
(%) 1st 2nd 3rd 4th 5th total 9:1 6.5 9.2 7.0 11.4 15.3 49.4 100 7:3 5.0 8.8 10.8 11.3 14.5 50.4 100 5:5 4.0 6.8 9.0 7.0 13.0 39.8 80 3:7 5.7 6.6 7.2 7.2 10.0 36.7 100 1:9 4.0 7.0 7.4 9.8 8.7 36.9 80 control group 7.0 9.7 10.6 10.8 12.5 50.6 80

Experimental Example 2: Confirmation of Additive Effect

By adding additives to the artificial feed prepared in Example 1, it was confirmed whether there was an effect on the growth characteristics of long-winged katydid.

Specifically, in the case of 1 to 3 years old, 1 g of fish meal, mulberry dross, Korean black raspberry dross, and blueberry dross were added to 3 g of artificial feed, respectively, and 4 to 5 years old. In the case of , 3g was supplied to 5g of artificial feed, and the growth characteristics were compared using artificial feed without additives as a control while replacing it every 2 to 3 days.

As a result, As shown in Table 7, when artificial feeds containing fish meal, mulberry meal, raspberry meal, and blueberry meal were added, respectively, the development period of nymphal larvae was shorter by about 4 to 8 days compared to the control group without additives. found to grow rapidly. By additives, it was confirmed that the development period was short in the order of mulberry peel > blueberry peel > raspberry peel > fishmeal. These results are presumed to be due to the fact that the growth rate is accelerated by the additives stimulating feeding.

additive nymph development period survival rate
(%) 1st 2nd 3rd 4th 5th Total fishmeal 4.0 7.0 7.4 8.6 11.8 38.8 100 Audi Park 4.3 5.8 5.2 9.2 11.0 35.5 100 raspberry pumpkin 3.8 6.2 7.6 9.2 11.3 38.1 100 blueberry peel 3.6 5.8 7.8 9.0 11.8 36.4 100 control group 5.8 7.4 8.2 8.6 12.2 43.2 100

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (10)

An artificial feed composition for breeding Gampsocleis ussuriensis, comprising a vegetable food source, an animal food source, pyridoxine, calcium pantothenate and crotonic acid.
According to claim 1,
The animal food source is mealworm or cricket, artificial feed composition.
According to claim 1,
The vegetable food source is any one or more selected from cabbage, Chinese cabbage, corn and kale, artificial feed composition.
According to claim 1,
The vegetable food source and the animal food source are mixed in a weight ratio of 2:8 to 4:6, artificial feed composition.
According to claim 1,
Sorbic acid, gallic acid, corn starch, ascorbic acid, citric acid, carrageenan, cellulose, soybean meal, agar and water are further added to the composition Characterized in that it comprises, artificial feed composition.
According to any one of claims 1 to 5,
Characterized in that the composition further comprises at least one of fish meal, mulberry meal, raspberry meal and blueberry meal, artificial feed composition.
According to claim 6,
The artificial feed composition, wherein at least one of the fish meal, mulberry meal, raspberry meal and blueberry meal and the artificial feed composition are mixed in a weight ratio of 3: 5 to 9.
As a method for producing a long-winged katydid artificial feed composition,
(a) mixing a vegetable food source, an animal food source, pyridoxine, calcium pantotenate, and crotonic acid;
(b) sterilizing the mixture; and
(c) removing air bubbles from the sterilized mixture and then refrigerating to prepare an artificial feed composition for breeding katydid.
According to claim 8,
In step (a), sorbic acid, gallic acid, corn starch, ascorbic acid, citric acid, carrageenan, cellulose, soybean meal, agar And characterized in that the mixture further comprises water, the manufacturing method.
An artificial breeding method for long-winged katydids, characterized in that the artificial feed composition of claim 1 is fed to and reared.

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