KR102635347B1 - Breeding Artificial Diet Composition for breeding Gampsocleis ussuriensis And Artificial Breeding Method of Gampsocleis ussuriensis - Google Patents

Abstract

The present invention relates to an artificial feed composition for rearing long-winged katydids and an artificial rearing method for long-winged katydids using the same.
The artificial feed composition for raising long-winged katydids according to the present invention is easier to feed compared to conventional vegetable or animal natural feeds, and there is no difference in feeding amount and growth characteristics, so it can be usefully used for easily breeding katydids for home and exhibition purposes. .

Description

Artificial feed composition for breeding long-winged katydid and artificial breeding method of long-winged katydid using the same {Breeding Artificial Diet Composition for breeding Gampsocleis ussuriensis And Artificial Breeding Method of Gampsocleis ussuriensis}

The present invention relates to an artificial feed composition for rearing long-winged katydids and an artificial rearing method for long-winged katydids using the same.

Emotional insects are insects used in theme parks, experience farms, etc., including pets and insects for experiential learning, and are insects used in the 6th industry by activating human cognitive, emotional, and physical functions. Recently, as the size of the companion animal market has expanded due to population aging, expansion of single-person households, and increased leisure activities, and the emotional healing effects of insects have been discovered, interest in emotional insects and healing agriculture is increasing.

However, although there is a high possibility of continued development of the emotional insect market, only about 50 types of insects have been developed and mass production technology is lacking. There are 293 domestic insect sales stores nationwide, but the insects sold are rhinoceros beetles, stag beetles, There are only about 10 species, including flower radish, so growth is limited due to lack of product diversity.

Meanwhile, the katydid is a representative vocal insect, and since ancient times in Korea, there has been a custom of raising it in houses made of barley and appreciating its cries during the summer. It is an insect that is helpful in cultivating human emotions. In China, katydids are also used for food, and katydid fighting is an important folk game of the Chinese people, and groups such as the katydid association and the katydid club have been established for the purpose of research, development, use, observation, and entertainment of katydids. Insects such as katydids are becoming established as products for relaxation/leisure.

However, despite this increasing demand for katydids, there are no reports on the detailed ecology of katydid insects and mass-rearing techniques for year-round production, and there has been no research on commercialization other than katydid houses for sound appreciation. The status of domestic research includes a taxonomic review of Korean katydids and studies on the life history of katydids, but research on the ecological characteristics and mass-rearing techniques of katydids is lacking.

Therefore, it is necessary to develop breeding technology for the katydid insects desired by farmers and consumers, and it is necessary to establish a support system for emotional insect education farms and expand ways to incorporate insects in connection with departments related to psychological healing.

Under this background, the present inventors made extensive research efforts to develop artificial feed for mass breeding long-winged katydid, one of the katydid family insects, and as a result, raised long-winged katydid using artificial feed containing animal and vegetable food sources. The present invention was completed by confirming that it is easy to feed and has good growth compared to live feed.

Republic of Korea Patent Publication No. 10-2020-0072129

The present invention aims to solve the above-described problems and other problems associated therewith.

An exemplary object of the present invention is to breed long-winged katydid (Gampsocleis ussuriensis), including plant food sources, animal food sources, pyridoxine, calcium pantothenate, and crotonic acid. The purpose is to provide an artificial feed composition for use.

Another exemplary object of the present invention is to provide a method for manufacturing long-winged katydid artificial feed comprising the following steps.

(a) mixing vegetable food sources, animal food sources, pyridoxine, calcium pantothenate, and crotonic acid;

(b) sterilizing the mixture; and

(c) removing air bubbles from the sterilized mixture and then refrigerating it to prepare an artificial feed composition for raising long-winged katydids.

Another exemplary object of the present invention is to provide an artificial breeding method of long-winged katydid, characterized in that the artificial feed composition is fed to the long-winged katydid for rearing.

The technical problem to be achieved according to the technical idea of the invention disclosed in this specification is not limited to the problem to solve the problems mentioned above, and other problems not mentioned can be clearly understood by those skilled in the art from the description below. There will be.

This is explained in detail as follows. Meanwhile, each description and embodiment disclosed in the present application may also be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in this application fall within the scope of this application. Additionally, the scope of the present application cannot be considered limited by the specific description described below.

As an aspect for achieving the above object, the present invention provides a long-winged katydid containing plant food sources, animal food sources, pyridoxine, calcium pantothenate, and crotonic acid. Provides an artificial feed composition for breeding.

The term "long-winged katydid" in the present invention is an insect of the order Orthoptera and the genus Katydid. It is similar to the katydid of the same genus, but has a smaller body shape and mainly inhabits bushes, grasslands, agricultural lands, riversides, embankments, and mountain valleys. It is distributed throughout the Korean Peninsula and is also known to be distributed in Hokkaido, Japan (Hokkaido), northeastern China, and the Russian Far East.

In the present invention, the artificial feed composition for rearing long-winged katydid refers to a feed composition that is artificially manufactured and mixes various necessary nutrients of long-winged katydid, and can exhibit growth characteristics better than natural feed when fed.

In the present invention, the animal food source may be mealworms or crickets, and may specifically be mealworms, but is not limited thereto. The animal food source of the present invention may be dried mealworms or crickets or dried powder, but is not limited thereto.

In the present invention, the vegetable food source may be any one selected from cabbage, Chinese cabbage, corn, and kale, and may specifically be cabbage, but is not limited thereto.

In the present invention, the vegetable food source may be seedlings grown for 20 to 30 days by pot-sown seeds of the plants.

In the present invention, the vegetable food source and the animal food source may be mixed at a weight ratio of 9:1 to 1:9, for example, may be mixed at a weight ratio of 2:8 to 4:6, for example It may be mixed at a weight ratio of 3:7, but is not limited thereto.

In the present invention, the pyridoxine serves to strengthen amino acid metabolism by providing vitamin B6, the calcium pantothenate is a composition for strengthening calcium salts to grow tissues, and the crotonic acid is an unsaturated fatty acid.

In the present invention, the pyridoxine and calcium pantothenate may be mixed at a weight ratio of 30:1 to 5:1, and the calcium pantothenate and crotonic acid may be mixed at a weight ratio of 1:50 to 1:15. The pyridoxine and crotonic acid may be mixed at a weight ratio of 1:5 to 1:1.

In the present invention, the composition includes an artificial feed composition for long-winged katydid, including sorbic acid, gallic acid, corn starch, ascorbic acid, citric acid, carrageenan, cellulose, It may further include soybean meal, agar, and water.

In the present invention, the sorbic acid acts as a nutrient and can be added to artificial feed to suppress microbial growth and prevent spoilage. The gallic acid is a reducing agent for improving cholesterol, the corn starch is used as a type of starch that provides viscosity or adhesiveness to artificial feed, and the ascorbic acid is vitamin C, which acts as an antioxidant and supplements the vitamins of long-winged katydid. For this purpose, the citric acid is a composition that acts as a preservative for artificial feed and promotes calcium absorption. The carrageenan is a composition that acts as a stabilizer added to artificial feed to give it adhesiveness and gelling, and the cellulose acts as a digestive agent when the long-winged katydid consumes artificial feed. The soybean meal is a protein source, and the agar serves as a gelling agent for artificial feed.

In the present invention, the sorbic acid and gallic acid may be mixed at a weight ratio of 1:3 to 1:2, and the ascorbic acid and citric acid may be mixed at a weight ratio of 5:12 to 7:10. Carrageenan and cellulose may be mixed at a weight ratio of 2:1 to 4:1, and the soybean meal and agar may be mixed at a weight ratio of 20:1 to 25:1.

In the present invention, the artificial feed composition for raising long-winged katydids may be divided into individual groups according to the purpose, manufactured, and then mixed. For example, the first composition containing sorbic acid, pyridoxine, calcium pantothenate, and gallic acid as nutrients. group, the second group containing crotonic acid, ascorbic acid and citric acid as a preservative, the third group containing carrageenan and cellulose as an adhesive, and the fourth group containing soybean meal as a protein source and then mixed. may be

In the present invention, the artificial feed composition for rearing long-winged katydid may further include one or more of fishmeal, mulberry meal, bokbunja, and blueberry meal as an additive. These additives are intended to increase the nutritional and functional components of artificial feed, and the addition of additives can improve the growth characteristics of long-winged katydid.

The additive and artificial feed composition may be mixed at a weight ratio of 3:5 to 9, but is not limited thereto.

The additives may be mixed into the artificial feed composition or supplied separately from the artificial feed composition.

Specifically, in an experimental example of the present invention, when both vegetable and animal food sources were included in the long-winged katydid artificial feed, the growth period was shorter and growth was smooth compared to the case where it was not, and the mixing ratio of vegetable and animal food sources was lower. It was confirmed that the total feeding amount was highest and the development period was short at 3:7, and when additives were added to the artificial feed, the development period was confirmed to be significantly reduced compared to the control group.

Based on these test examples, it was confirmed that the artificial feed composition for raising long-winged katydids of the present invention can be usefully used for raising long-winged katydids.

In another aspect of the present invention, the present invention provides a method for producing a long-winged katydid artificial feed composition comprising the following steps.

(a) mixing vegetable food sources, animal food sources, pyridoxine, calcium pantothenate, and crotonic acid;

(b) sterilizing the mixture; and

(c) removing air bubbles from the sterilized mixture and then refrigerating it to prepare an artificial feed composition for raising long-winged katydids.

In the present invention, the production method includes sorbic acid, gallic acid, corn starch, ascorbic acid, citric acid, carrageenan, cellulose, and soybean meal ( It may be mixed by further including soybean meal, agar, and water.

In another aspect of the present invention, the present invention provides an artificial breeding method of long-winged katydid, characterized in that the artificial feed composition is fed to the long-winged katydid for rearing.

The artificial feed composition for raising long-winged katydids according to the present invention is easier to feed compared to conventional vegetable or animal natural feeds, and there is no difference in feeding amount and growth characteristics, so it can be usefully used for easily breeding katydids for home and exhibition purposes. .

Figure 1 shows a long-winged katydid artificial feed composition prepared according to the present invention.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1: Preparation of artificial feed composition according to the present invention

1-1. Food source selection

To select the food source of artificial feed for raising long-winged katydids, the development characteristics were compared when vegetable food sources were provided alone and when vegetable food sources and animal food sources were mixed. Arrowroot, cucumber, radish, cabbage, Chinese cabbage, kale, and corn were used as vegetable food sources, and mealworms were used as animal food sources. Vegetable food sources were provided by pot-sown each plant seed and 20 to 30-day-old seedlings were placed in pots. Mealworms, which were animal food sources, were supplied in groups of 5 to 6 in each container and resupplied when all were consumed. Long-winged katydids were placed in a 20 x 20 x 20 cm breeding box and supplied with each food source, and their developmental characteristics were compared.

As a result, as shown in Table 1, when only plant food sources were supplied, the development period was longer and the survival rate was lower than when both plant and animal food sources were supplied. Cabbage and Chinese cabbage were found to be lower compared to arrowroot and cucumber. The survival rate was found to be relatively high. In the case of radish, the survival rate was the same as cabbage and Chinese cabbage, but the growth period was found to be very long.

food source Nymph development period (day) Viability rate (%) vegetable animal nature 1st instar 2nd instar 3rd spirit 4th instar 5th instar total Kudzu - 10 17.6 9.8 8 - 45.4 0 cucumber - 12 20 12.8 19 - 63.8 20 cabbage - 11 16 12 13.8 12 64.8 60 radish - 11.5 20.6 11.6 18.2 12.3 74.2 60 napa cabbage - 12 18 10.5 13.3 12.7 66.5 60 napa cabbage Mealworm 12 14 10.5 13.3 12.7 49.8 100 cabbage Mealworm 11 16 12 11.8 12 50.8 80 Kale Mealworm 11.2 13.2 12.5 11 12.5 47.9 80 corner mealworm 11.5 13.5 11.2 10.5 12.3 46.7 80

In addition, the weight of long-winged katydid nymphs when vegetable or animal food sources are provided alone (Table 2), and the weight of long-winged katydid nymphs and adults when a mixture of animal and vegetable food sources is provided ( Table 3) was compared.

As a result, as shown in Tables 2 and 3, when only vegetable food sources were supplied, the long-winged katydid of the 5th instar could not be identified in the case of arrowroot and cucumber due to poor growth, and in the case of cabbage, radish, and Chinese cabbage, they were also supplied together with mealworms. It was confirmed that the weight decreased compared to when the child was born and that the growth was stunted.

It was confirmed that when only mealworms, an animal food source, were supplied, the weight of the nymphs decreased compared to when they were supplied with a plant food source, resulting in poor growth. In addition, when animal food sources and vegetable food sources were supplied together, it was found that good growth was possible in all cabbage, cabbage, kale, and corn when compared depending on the type of vegetable food source. As a result, it was confirmed that the development of long-winged katydids was superior when vegetable or animal food sources were supplied together rather than provided alone.

food source Nymph weight (g) vegetable animal nature 1st instar 2nd instar 3rd spirit 4th instar 5th instar Kudzu - 0.17 0.33 0.37 0.42 - cucumber - 0.17 0.23 0.3 0.46 - cabbage - 0.18 0.31 0.55 0.8 1.62 radish - 0.18 0.28 0.4 0.56 1.56 napa cabbage - 0.16 0.3 0.45 0.79 1.55 - mealworm 0.16 0.31 0.65 0.89 1.86

food source Nymph weight (g) Adult weight (g) vegetable animal nature 1st instar 2nd instar 3rd spirit 4th instar 5th instar cancer number napa cabbage mealworm Weight (g) 0.6 0.6 0.8 2.1 2.2 2.8 3 cabbage mealworm Weight (g) 0.6 0.6 1.5 1.8 2.5 3 2.4 Kale mealworm Weight (g) 0.9 1.3 1.7 2.4 2.1 2.7 2.5 corner mealworm Weight (g) 0.4 0.7 2.1 2.2 2.5 2.4 2

1-2. Artificial feed manufacturing

In order to manufacture artificial feed for raising long-winged katydids, the feed composition shown in Table 4 below was prepared, including vegetable food sources and animal food sources, referring to the results in 1-1 above. Vegetable food sources and animal food sources were prepared at ratios (weight ratios) of 9:1, 7:3, 5:5, 3:7, and 1:9, respectively.

The first group, including sorbic acid, pyridoxine, calcium pantothenate, and gallic acid, serves as a nutritional supplement for long-winged katydid, and the second group, including crotonic acid, ascorbic acid, and citric acid, serves as a preservative for artificial feed. The third group, including carrageenan and cellulose, acts as an adhesive to ensure that the compositions mix well, and the fourth group, including soybean meal, is a protein source. Corn starch and agar were added to maintain viscosity during kneading.

group Feed composition Weight (g) One Sorbic acid One pyridoxine 0.6 Calcium pentothenate (Ca-pantotenate) 0.05 Gallic acid 2.3 corn starch 30 2 Crotonic acid 2 ascorbic acid 7.5 citric acid 9.1 corn starch 50 3 Carrageenine 30 cellulose 10 4 soybean meal 235 Vegetable-to-animal ratio 9:1 7:3 5:5 3:7 1:9 Plant-based food sources (cabbage, Chinese cabbage, corn or kale) 144 112 80 48 16 Animal food source (mealworms) 16 48 80 112 144 Agar 10 5 water 700mL

After preparing groups 1, 2, 3, and 4, respectively, they were mixed with water, kneaded in a blender, and sterilized at 120°C for 20 minutes. Afterwards, air bubbles were removed, cooled on a clean bench, and stored in the refrigerator. As a result, long-winged katydid artificial feed was prepared as shown in Figure 1.

Experimental Example 1: Confirmation of long-winged katydid development characteristics by mixing ratio

1-1. Comparison of nymph feeding amounts

Long-winged katydids were fed artificial feed with different mixing ratios and their growth characteristics were confirmed.

Specifically, the 1st to 3rd instars of long-winged katydids were fed 3 to 5g of artificial feed, and the 4th to 5th instars were fed 5 to 10g of artificial feed, and the feeding amount was checked by replacing it every 2 to 3 days.

As a result of analyzing the nymph feeding amount by varying the mixing ratio of the vegetable food source and the animal food source of the artificial feed, as shown in Table 5, the feeding amount when the ratio of cabbage, a vegetable food source, to mealworm, an animal food source, at the 5th instar was 3:7. This was 12.5g, and the average daily intake was highest at 1.8g, and the total intake was also highest at 23.7g when the ratio was 3:7.

mixing ratio
(Plant:Animal) Feeding amount (g) average
1 day
eating amount
(g) 1st instar 2nd instar 3rd spirit 4th instar 5th instar total T M T M T M T M T M T M 9:1 2.0 0.7 2.6 0.7 2.9 0.7 4.1 0.8 11.6 1.7 23.1 1.0 1.0 7:3 1.8 0.6 2.7 0.7 2.9 0.7 4.0 0.8 10.7 1.5 22.1 0.9 0.9 5:5 2.1 0.7 2.5 0.6 2.7 0.7 3.8 0.8 9.4 1.6 22.3 0.9 0.9 3:7 2.1 0.7 2.3 0.6 2.5 0.6 4.2 0.8 12.5 1.8 23.7 1.0 1.0 1:9 2.2 0.7 2.1 0.5 2.3 0.6 3.1 0.6 6.1 1.5 21.6 0.8 0.8

T: total amount consumed, M: average daily amount consumed

1-2. Comparison of nymph development period and survival rate

The nymph development period and survival rate were analyzed by varying the mixing ratio of vegetable and animal food sources in artificial feed. As a control group, cabbage and mealworms, which are living organisms of animals and plants, were fed and compared. As a result, as shown in Table 6, when artificial feed was fed with a ratio of plant food source: animal food source of 3:7, the live birth rate was 100% and the nymph development period was 36.7 days, which was the longest compared to other mixing ratios. It was confirmed that the long-winged katydid appeared briefly and grew quickly.

mixing ratio
(Plant:Animal) Nymph development period (day) survival rate
(%) 1st instar 2nd instar 3rd spirit 4th instar 5th instar total 9:1 6.5 9.2 7.0 11.4 15.3 49.4 100 7:3 5.0 8.8 10.8 11.3 14.5 50.4 100 5:5 4.0 6.8 9.0 7.0 13.0 39.8 80 3:7 5.7 6.6 7.2 7.2 10.0 36.7 100 1:9 4.0 7.0 7.4 9.8 8.7 36.9 80 control group 7.0 9.7 10.6 10.8 12.5 50.6 80

Experimental Example 2: Confirmation of additive effect

Additives were added to the artificial feed prepared in Example 1 to determine whether they were effective in the development characteristics of long-winged katydid.

Specifically, for 1st to 3rd instars, 3g of artificial feed plus 1g each of fish meal, mulberry dross, Korean black raspberry dross, and blueberry dross, for 4th to 5th instars. In the case of , 3g was supplied to 5g of artificial feed, replaced at intervals of 2 to 3 days, and the growth characteristics were compared using artificial feed without added additives as a control.

As a result, As shown in Table 7, when artificial feed containing fish meal, mulberry meal, bokbunja meal, and blueberry meal was fed, the long-winged katydid nymph development period was shorter by about 4 to 8 days compared to the control group without additives. It was confirmed to be growing rapidly. By additive, it was confirmed that the growth period was short in the following order: mulberry meal > blueberry meal > bokbunja meal > fishmeal. These results are presumed to be due to the additive stimulating feeding and accelerating the growth rate.

additive Nymph development period survival rate
(%) 1st instar 2nd instar 3rd spirit 4th instar 5th instar Total fishmeal 4.0 7.0 7.4 8.6 11.8 38.8 100 mulberry 4.3 5.8 5.2 9.2 11.0 35.5 100 Bokbunja Park 3.8 6.2 7.6 9.2 11.3 38.1 100 Blueberry Park 3.6 5.8 7.8 9.0 11.8 36.4 100 control group 5.8 7.4 8.2 8.6 12.2 43.2 100

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. In this regard, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed as including the meaning and scope of the patent claims described below rather than the detailed description above, and all changes or modified forms derived from the equivalent concept thereof are included in the scope of the present invention.

Claims (10)

An artificial feed composition for raising long-winged katydid (Gampsocleis ussuriensis), comprising a vegetable food source, an animal food source, pyridoxine, calcium pantothenate, and crotonic acid,
The vegetable food source is cabbage or Chinese cabbage,
An artificial feed composition wherein the vegetable food source and the animal food source are mixed at a weight ratio of 3:7 to 4:6.
According to paragraph 1,
An artificial feed composition wherein the animal food source is mealworms or crickets.
delete delete According to paragraph 1,
Sorbic acid, gallic acid, corn starch, ascorbic acid, citric acid, carrageenan, cellulose, soybean meal, agar and water are added to the composition. An artificial feed composition comprising:
According to any one of paragraphs 1, 2, and 5,
An artificial feed composition, characterized in that the composition further includes one or more of fish meal, mulberry meal, bokbunja meal, and blueberry meal.
According to clause 6,
The artificial feed composition is one or more of the fish meal, mulberry meal, bokbunja meal, and blueberry meal and the artificial feed composition is mixed at a weight ratio of 3:5 to 9.
A method for producing a long-winged katydid artificial feed composition,
(a) mixing vegetable food sources, animal food sources, pyridoxine, calcium pantothenate, and crotonic acid;
(b) sterilizing the mixture; and
(c) preparing an artificial feed composition for raising long-winged katydids by removing air bubbles from the sterilized mixture and storing it in refrigeration,
The vegetable food source is cabbage or Chinese cabbage,
A manufacturing method wherein the vegetable food source and the animal food source are mixed at a weight ratio of 3:7 to 4:6.
According to clause 8,
In step (a), sorbic acid, gallic acid, corn starch, ascorbic acid, citric acid, carrageenan, cellulose, soybean meal, and agar. And a manufacturing method characterized in that the mixture further includes water.
An artificial breeding method of long-winged katydid, characterized in that the artificial feed composition of paragraph 1 is fed to long-winged katydid for rearing.