KR20200072129A - Breeding Artificial Diet composition for Growth Enhancing of Protaetia brevitarsis larva - Google Patents
Breeding Artificial Diet composition for Growth Enhancing of Protaetia brevitarsis larva Download PDFInfo
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Abstract
Description
본 발명은 흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물에 관한 것으로서, 보다 상세하게는 기존 흰점박이꽃무지 사료에 비해 현저하게 흰점박이꽃무지 유충의 생장을 촉진시켜 발육기간을 단축시킴과 동시에 생존율도 70% 이상인 흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물에 관한 것이다.The present invention relates to an artificial feed composition for promoting growth of white-spotted larvae larvae, more specifically, compared to the existing white-spotted larvae feed, to promote the growth of white-spotted larvae larvae to shorten the development period and at the same time It relates to an artificial feed composition for promoting growth of white spotted larvae larvae having a survival rate of 70% or more.
최근 국제식량농업기구(FAO)에서는 질병이나 환경오염으로 인한 식량부족 문제를 해결하기 위한 정책으로 미래식량자원으로서의 식용곤충의 활성화방안을 발표한 바 있고, 이에 대한 관심이 전 세계적으로 증대되고 있다.Recently, the International Food and Agriculture Organization (FAO) has announced a plan to remedy food insects as a future food resource as a policy to solve the food shortage caused by disease or environmental pollution, and interest in it has increased worldwide.
예로부터 곤충은 약용으로 사용해 왔으며, 그 중 누에, 메뚜기, 흰점박이꽃무지 유충(굼벵이), 지네 등은 당뇨, 염증성 질환, 간질환 및 동맥경화 등의 만성 질환 치료에 효과적이라는 연구결과가 보고되었다.Since ancient times, insects have been used for medicinal purposes, among which silkworms, grasshoppers, white spotted larvae (slugs), centipedes, etc., have been reported to be effective in treating chronic diseases such as diabetes, inflammatory diseases, liver disease, and arteriosclerosis. .
상기 식용곤충의 하나인 흰점박이꽃무지(Protaetia brevitarsis)는 민간 및 동의보감 등의 전통 한방의서에서 "제조" 또는 "굼벵이" 라는 속명으로 불리고 있는 딱정벌레목, 풍뎅이과, 꽃무지아과에 속한 초식성 곤충이다. 흰점박이꽃무지의 유충은 크기에 따라 1~3령으로 분류하는데, 3령 유충은 평균 머리폭이 4.3~4.5 ㎜ 전후가 되며, 몸길이는 25~37 ㎜로써, 이 시기의 유충의 몸은 유백색이며 전체에 황색의 짧은 털들이 촘촘하게 나있으며, 몸의 크기에 비해 머리 크기가 작고 다리가 발달되지 않아 이동시 등을 이용하여 이동한다.One of the edible insects, white spotted radish ( Protaetia brevitarsis ), is a herbivorous insect belonging to the Coleoptera, Chafer, and Asteraceae , which are called "manufacturing" or "slugs" in traditional Chinese medicines such as folk and Donguibogam. . Caterpillars of white-spotted ignorant are classified into 1 to 3 ages according to their size, and the 3rd-large larvae have an average head width of about 4.3 to 4.5 mm and a body length of 25 to 37 mm. The yellow short hairs are densely packed in the whole, and the size of the head is small compared to the size of the body, and the legs are not developed.
흰점박이꽃무지 외의 꽃무지류(Protaetia spp.)로는 잔꽃무지, 참꽃무지, 검정꽃무지, 홀쭉꽃무지, 풀색꽃무지, 넓적꽃무지, 범꽃무지, 참넓적꽃무지, 점박이꽃무지, 애초록꽃무지, 만주점박이꽃무지, 큰자색호랑꽃무지 등이 있으나 각각 별개의 곤충으로 인정된다. The flower radishes ( Protaetia spp.) other than white-spotted flowers, such as small-flowered flowers, true-flowered flowers, black-flowered flowers, blue-flowered flowers, full-colored flowers, broad-flowered flowers, wild-flowered flowers, truly broad-flowered flowers, spotted flowers, There are flower ignorance, Manju spotted flower ignorance, and large purple tiger flower ignorance, but each is recognized as a separate insect.
흰점박이꽃무지는 성충이 알을 낳아 부화해서 1령에서 3령을 거쳐 종령을 지나 번데기가 되며 번데기 후에 성충이 되는 완전탈바꿈을 하는 곤충에 하는 것으로, 1령에서 3령까지의 발육기간은 약 4~5개월이 걸리며, 어린 유충의 성장 속도에 가장 중요한 영향을 미치는 것은 사육환경으로서 온도, 먹이, 습도 등의 요인들이 있다. 특히, 흰점박이꽃무지는 3령의 성숙유충 상태로 월동하며, 성충은 주간에 활동하고 복숭아, 배 등의 성숙한 과일이나 옥수수, 상수리 나무 등의 즙액을 먹이로 하며, 이들의 유충은 퇴비나 건초더미 등의 유기물이 풍부한 부식성 토양속에서 서식한다. The white-spotted ignorance is done by insects that transform into complete insects that become adults after the pupae and become pupae after breeding from 1 to 3 years after the adult eggs lay and lay eggs. The period of development from 1 to 3 years is about It takes 4 to 5 months, and the most important factor affecting the growth rate of young larvae is the breeding environment, which includes factors such as temperature, food, and humidity. In particular, white-spotted ignorance is overwhelmed in the state of mature larvae of the third age. Adults are active during the daytime and feed on mature fruits such as peaches, pears, or juices such as corn and oak trees, and their larvae are compost or hay. Inhabits in corrosive soils rich in organic matter such as piles.
최근에는 이러한 흰점박이꽃무지의 약용성에 관심이 집중되면서 많은 사람들이 흰점박이꽃무지를 대량으로 채집하려고 하나 자연산의 채집에는 어려움이 많다.In recent years, as attention is focused on the medicinal properties of these white spotted ignorance, many people try to collect white spotted ignorance in large quantities, but it is difficult to collect natural products.
흰점박이꽃무지 사육농가가 곤충사육 농가 중 3/4으로 가장 많은 비율을 차지하고 있지만, 아직 사료에 대한 과학적 근거를 제공하고 있지는 않다. 이에 흰점박이꽃무지의 인공사육방법에 대한 관심이 점차 늘고 있지만, 아직까지는 흰점박이꽃무지를 대량생산하기에는 체계적인 연구가 부족한 실정이다.White spotted flower farms account for 3/4 of insect farms, but they do not yet provide scientific evidence for feed. Accordingly, although interest in artificial breeding methods of white spotted ignorance is gradually increasing, systematic studies have not yet been conducted to mass-produce white spotted ignorance.
본 발명의 목적은 기존 흰점박이꽃무지 사료에 비해 현저하게 흰점박이꽃무지 유충의 생장을 촉진하여 발육기간을 단축함과 동시에 생존율도 70% 이상인 흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물을 제공하는 데에 있다.The object of the present invention is to promote the growth of the white spotted flower larvae significantly compared to the existing white spotted flower plain feed, while shortening the development period and at the same time, the artificial feed composition for promoting the growth of the white spotted flower plain larvae more than 70%. It is in providing.
상기 목적을 달성하기 위한 본 발명의 흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물은, 톱밥, 카제인, 옥수수전분, 밀기울로 구성된 혼합물을 발효한 발효물을 포함하는 것이 특징이며, 보다 바람직하게 상기 혼합물은 상기 톱밥 60~80 중량%, 상기 카제인 10~25 중량%, 상기 옥수수전분 5~15 중량%, 상기 밀기울 1~5 중량%로 구성되는 것이 특징이다. Artificial feed composition for promoting the growth of white spotted flower larvae of the present invention for achieving the above object is characterized in that it comprises a fermentation product fermented with a mixture consisting of sawdust, casein, corn starch, wheat bran, more preferably the The mixture is characterized by consisting of 60 to 80% by weight of the sawdust, 10 to 25% by weight of the casein, 5 to 15% by weight of the corn starch, and 1 to 5% by weight of the wheat bran.
또 다른 본 발명인 흰점박이꽃무지 유충의 생장 촉진용 인공사료의 제조방법은, 톱밥, 카제인, 옥수수전분, 밀기울로 혼합된 혼합물을 제조하는 제1단계 및, 상기 혼합물에 물을 넣은 후 발효하여 흰점박이꽃무지 유충의 생장 촉진용 인공사료를 제조하는 제2단계를 포함하는 것이 특징이며, 상기 제1단계의 혼합물은 상기 톱밥 60~80 중량%, 상기 카제인 10~25 중량%, 상기 옥수수전분 5~15 중량%, 상기 밀기울 1~5 중량%로 구성되는 것이 특징이다. Another method of manufacturing artificial feed for promoting growth of white spotted flower larvae according to the present invention is a first step of preparing a mixture of sawdust, casein, corn starch and wheat bran, and fermenting the mixture after adding water to the white It characterized in that it comprises a second step of preparing an artificial feed for promoting growth of spotted flower larvae, the mixture of the first step is 60 to 80% by weight of the sawdust, 10 to 25% by weight of the casein, the corn starch 5 It is characterized by being composed of ˜15% by weight, and 1 to 5% by weight of the bran.
또한, 상기 제2단계의 인공사료 제조시, 상기 혼합물에 혼합물 100 중량부를 기준으로 40~50 중량부의 물을 넣는 것이 특징이며, 상기 발효는 20~30℃에서 2~3개월동안 이루어지는 것이 특징이다. In addition, when preparing the artificial feed of the second step, it is characterized in that 40 to 50 parts by weight of water is added to the mixture based on 100 parts by weight of the mixture, and the fermentation is characterized by being performed at 20 to 30°C for 2-3 months. .
또 다른 본 발명인 흰점박이꽃무지 유충의 생장 촉진용 인공사료는, 상기 제조방법에 의해 제조되는 것이 특징이며, 이러한 상기 인공사료를 흰점박이꽃무지 유충에게 급이하여 흰점박이꽃무지 유충을 사육하는 것이 특징이다.Another present invention artificial feed for promoting growth of white spotted flower larvae is characterized by being manufactured by the above manufacturing method, and feeding the artificial feed to white spotted flower larvae to breed white spotted flower larvae. It is characteristic.
본 발명이 이루고자 하는 기술적 과제들은 이상에서 언급한 기술적 과제들로 제한되지 않으며, 언급되지 않은 다른 기술적 과제들은 아래의 기재로부터 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 명확하게 이해될 수 있을 것이다.The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems that are not mentioned can be clearly understood by a person having ordinary knowledge in the technical field to which the present invention belongs from the following description. There will be.
본 발명에 의해, 흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물가 제공됨에 따라, 기존 흰점박이꽃무지 사료에 비해 현저하게 흰점박이꽃무지 유충의 생장을 촉진하여 발육기간을 단축함과 동시에 생존율도 70% 이상을 나타냄에 따라 앞으로 흰점박이꽃무지의 산업적 이용을 위해서 속성으로 사육이 가능해져 식용곤충 사육농가에 도움을 줄 것으로 기대된다.According to the present invention, as the artificial feed composition for promoting the growth of white spotted flower larvae is provided, the growth rate of the white spotted flower larvae is significantly accelerated compared to the existing white spotted flower larvae feed, thereby shortening the development period and survival rate. As it shows more than 70%, it is expected that it will be able to grow as an attribute for industrial use of white-spotted flower plains in the future, which is expected to help edible insect breeding farms.
도 1은 각 사료들의 발효기일에 따른 온도변화를 나타낸 그래프이다.
도 2는 각 사료에 따른 흰점박이꽃무지 유충의 생존율을 나타낸 그래프이다.1 is a graph showing the temperature change according to the fermentation date of each feed.
2 is a graph showing the survival rate of white spotted flower larvae according to each feed.
이하, 본 발명을 상세하게 설명하며, 본 발명의 요지를 불필요하게 흐릴 수 있는 공지 기능 및 구성에 대한 상세한 설명은 생략한다.Hereinafter, the present invention will be described in detail, and detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.
본 발명은 흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물에 관한 것으로써, 보다 상세하게는 기존 흰점박이꽃무지 사료에 비해 현저하게 흰점박이꽃무지 유충의 생장을 촉진시켜 발육기간을 단축시킴과 동시에 생존율도 70% 이상을 나타낼 수 있도록 톱밥, 카제인, 옥수수전분, 밀기울로 구성된 혼합물을 발효한 발효물을 포함하는 흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물을 제공하는 것이 특징이다.The present invention relates to an artificial feed composition for promoting growth of white-spotted larvae larvae, and more specifically, to shorten the development period by significantly promoting the growth of white-spotted larvae larvae compared to the existing white-spotted larvae feed. At the same time, it is characterized by providing an artificial feed composition for promoting growth of white-spotted flower larvae containing fermented fermented mixture of sawdust, casein, corn starch and bran so that the survival rate can be more than 70%.
상기 톱밥은 상기 인공사료 조성물의 주재료가 되는 성분으로, 참나무 톱밥을 사용하는 것이 바람직한데, 상기의 참나무로 이루어진 톱밥은 흰점박이 꽃무지 유충이 섭취하기 좋은 특성을 나타내며, 흰점박이 꽃무지 유충의 발육속도를 증진시키는 역할을 한다. 이러한 톱밥은 250메쉬 내지 350메쉬 범위의 크기인 것이 바람직한 것으로써, 상기 톱밥의 크기가 250메쉬 미만인 경우에는 유충이 먹이 섭식이 불량하고, 350메쉬를 초과하는 경우에는 입자가 작아 수분유지에 바람직하지 않다.The sawdust is a component that is a main ingredient of the artificial feed composition, and it is preferable to use oak sawdust. The sawdust made of oak exhibits good properties for white spotted flower larvae to ingest, and white spotted flower larvae develop. It serves to increase speed. Such sawdust is preferably in the range of 250 mesh to 350 mesh. When the size of the sawdust is less than 250 mesh, the caterpillars have poor feeding, and if it exceeds 350 mesh, the particles are small, which is undesirable for maintaining moisture. not.
상기 카제인(Casein)은 인공사료의 단백질원으로서 가장 널리 사용되고 있는 것으로써 시판되고 있는 다른 단백질원과 비교했을 때 순도가 높고, 곤충이 필수로 하는 아미노산 균형이 비교적 좋은 것으로 알려져 있다.The casein (Casein) is the most widely used as a protein source of artificial feed, it is known to have a high purity compared to other commercially available protein sources, and relatively good amino acid balance required by insects.
상기 옥수수전분은 흰점박이꽃무지 유충에 대한 소화흡수율이 높아 에너지원으로 쉽게 이용되며, 생육을 촉진시키는 역할을 한다. 또한, 다른 성분들과의 결착력을 증가시키고, 각 성분들의 비산을 방지하여 사료의 손실을 방지하는 효과가 있다.The corn starch has a high digestion and absorption rate against white spotted flower larvae and is easily used as an energy source, and serves to promote growth. In addition, it has the effect of increasing the binding force with other components, and preventing the loss of feed by preventing the scattering of each component.
상기 밀기울은 소맥피라고도 하며 밀을 빻아 체로 쳐서 남은 찌꺼기로 발효는 촉진하는데 일반적으로 사료용 소재로 많이 사용되고 있다.The wheat bran is also referred to as wheat flour, and is generally used as a feed material to promote fermentation by crushing wheat and sieving it.
이와 같이 본 발명은 상기와 같은 재료들을 사용하여 흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물을 제조하되, 영양소가 충분히 함유되어 있고 영양소간의 균형이 맞도록 하여 흰점박이꽃무지 유충의 생장을 촉진시킬 수 있도록 하기 위해, 더욱 바람직하게는 상기 흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물이 톱밥 60~80 중량%, 카제인 10~25 중량%, 옥수수전분 5~15 중량%, 밀기울 1~5 중량%로 구성된 혼합물을 발효한 발효물을 포함하는 것이 가장 좋다.As described above, the present invention prepares an artificial feed composition for promoting the growth of white spotted flower larvae using the above-mentioned materials, but promotes the growth of white spotted flower larvae by sufficiently containing nutrients and balancing the nutrients. In order to make it possible, more preferably, the artificial feed composition for promoting the growth of the white spotted flower larvae is 60-80% by weight of sawdust, 10-25% by weight of casein, 5-15% by weight of corn starch, 1-5 bran It is best to include a fermentation product that has fermented a mixture of weight percent.
설명하면, 상기 톱밥과 상기 밀기울은 곤충 사료용 소재로 통상적으로 사용되었던 소재들로써, 상기 톱밥의 함량이 60 중량% 미만일 경우에는 상대적으로 밀기울의 함량을 높여야 하는 바 경제적 부담이 발생되며, 80 중량%를 초과할 경우에는 상대적으로 상기 옥수수전분 및 카제인의 함량이 낮게 함유되는 바, 본 발명이 목적하는 흰점박이꽃무지 유충이 생장촉진효능을 얻지 못하게 된다. In other words, the sawdust and the bran are materials that were commonly used as a material for insect feed. When the content of the sawdust is less than 60% by weight, an economic burden arises to increase the content of the bran, and 80% by weight When exceeded, the corn starch and casein contents are relatively low, so that the white spotted flower radish larvae desired by the present invention cannot obtain the growth promoting effect.
또한, 상기 밀기울의 함량이 1 중량% 미만일 경우에는 영양소 간의 균형이 맞지 않아 흰점박이꽃무지 유충이 발육이 더디게 될 우려가 있게 되며, 5 중량%를 초과할 경우에는 5 중량%이하 함유되는 경우 대비 보다 상승된 효과는 얻지 못하면서, 경제적 부담만 발생될 우려가 있게 된다. In addition, when the content of the bran is less than 1% by weight, the balance between nutrients is not balanced, and there is a fear that the white spotted flower larva larvae may develop slowly, and when it exceeds 5% by weight, compared to the case where the content is less than 5% by weight There is a possibility that only an economic burden may be generated, while not obtaining a more elevated effect.
또한, 상기 카제인의 함량이 10 중량% 미만으로 함유되거나, 상기 옥수수 전분의 함량이 5 중량% 미만으로 함유될 경우에는 영양적 균형이 맞지 않아 본 발명이 목적하는 흰점박이꽃무지 유충이 생장촉진효능을 얻지 못하게 되며, 상기 카제인의 함량이 25 중량%를 초과하거나, 상기 옥수수 전분의 함량이 15 중량%를 초과할 경우에는 나머지 다른 재료들의 조성 비율이 맞지 않아 이 역시 흰점박이꽃무지 유충의 생장촉진능을 얻지 못하게 된다. 이는 하기 실시예를 통해 입증된다.In addition, when the content of the casein is less than 10% by weight, or the content of the corn starch is less than 5% by weight, the nutritional balance is not correct, so that the white spotted flower larva larvae desired by the present invention can promote growth. If the content of the casein exceeds 25% by weight, or when the content of the corn starch exceeds 15% by weight, the composition ratio of the other ingredients does not match, which also promotes the growth of white spotted flower larvae. You will not be able to gain abilities. This is demonstrated through the following examples.
이하, 본 발명인 흰점박이꽃무지 유충의 생장촉진용 인공사료의 제조방법에 대하여 구체적으로 설명하면 다음과 같다. Hereinafter, a method of manufacturing artificial feed for promoting growth of white spotted flower larvae of the present invention will be described in detail as follows.
1단계) 혼합물 제조Step 1) Preparation of the mixture
본 단계는 톱밥, 카제인, 옥수수전분, 밀기울로 혼합된 혼합물을 제조하는 것을 특징으로, 보다 바람직하게는 상기 톱밥 60~80 중량%, 상기 카제인 10~25 중량%, 상기 옥수수전분 5~15 중량%, 상기 밀기울 1~5 중량%로 구성되도록 혼합물을 제조하는 것이 흰점박이꽃무지 유충이 성장하기에 적합한 영양소가 충분히 함유되어 있고 영양소간의 균형이 맞아, 흰점박이꽃무지 유충의 발육을 좋게 하여 성장을 촉진시키게 된다.This step is characterized in that to prepare a mixture of sawdust, casein, corn starch, wheat bran, more preferably 60 to 80% by weight of the sawdust, 10 to 25% by weight of the casein, 5 to 15% by weight of the corn starch , To prepare the mixture to be composed of 1 to 5% by weight of the bran contains enough nutrients suitable for the growth of white-spotted flower larvae and the balance between nutrients, improves the growth of white-spotted flower-plain larvae. To promote.
2단계) 최종 흰점박이꽃무지 유충의 생장 촉진용 인공사료 제조Step 2) Manufacture of artificial feed for promoting growth of the final white spotted flower larvae
본 단계에서는 상기 혼합물에 물을 넣은 후 발효하여 최종 흰점박이꽃무지 유충의 생장 촉진용 인공사료를 제조하는 것이 특징이다.In this step, the mixture is fermented after adding water to produce an artificial feed for promoting growth of the final white spotted flower larvae.
이때, 상기 혼합물에는 혼합물 100 중량부를 기준으로 40~50 중량부의 물을 넣는 것이며, 상기 발효는 20~30℃에서 2~3개월동안 이루어지는 것이 특징이다. At this time, 40 to 50 parts by weight of water is added to the mixture based on 100 parts by weight of the mixture, and the fermentation is characterized by being performed at 20 to 30°C for 2-3 months.
설명하면, 상기 물은 상기 혼합물 100 중량부 기준으로 40 중량부 미만으로 함유될 경우에는 상기 재료들의 발효가 제대로 이루어지지 않아 본 발명이 목적하는 효능을 얻기 어려우며, 50 중량부를 초과할 경우에는 과도한 물함량으로 인해 오히려 유충이 폐사될 우려가 있게 된다. In other words, when the water is contained in an amount of less than 40 parts by weight based on 100 parts by weight of the mixture, fermentation of the materials is not properly performed, and thus it is difficult to obtain the desired efficacy of the present invention, and when it exceeds 50 parts by weight, excess water Rather, there is a possibility that the larvae die.
또한, 상기 발효는 2~3개월 동안 이루어지는 것을 특징으로, 2개월 미만으로 발효될 경우에는 재료들의 충분이 발효되지 않아 발효과정에서 생성되는 가스와 온도상승으로 인해 유충이 폐사되게 되며, 3개월 초과하여 발효할 경우에는 초과기간에 따른 상승된 효과를 얻지 못하는 바, 상기 발효기간은 2~3개월이 가장 적합하다.In addition, the fermentation is characterized in that it takes place for 2 to 3 months, and if it is fermented for less than 2 months, enough of the materials are not fermented, causing larvae to die due to gas and temperature generated during the fermentation process, and exceeding 3 months In the case of fermentation, it is impossible to obtain an elevated effect according to the excess period, and the fermentation period is 2 to 3 months.
또한, 상기 인공사료에는 곤충사료에 통상적으로 사용되는 방부제, 보존제, 항생제, 비타민 등의 미량 영양성분 등이 더 포함될 수 있다. 상기 방부제, 보존 제, 항생제, 미량 영양성분 등은 해당 기술 분야에서 통상의 지식을 가진 당업자가 통상적으로 사료에 첨가하는 함량으로 적의 선택하여 사용할 수 있다.In addition, the artificial feed may further include micronutrients such as preservatives, preservatives, antibiotics, and vitamins commonly used in insect feed. The preservatives, preservatives, antibiotics, micronutrients, etc. can be used by selecting the enemy in a content that is normally added to the feed by those skilled in the art.
이렇게 제조된 상기 본 발명의 흰점박이꽃무지 유충의 생장촉진용 인공사료는 흰점박이꽃무지 유충에 급이하도록 함으로써 생존율도 70%이상으로 높이고, 발육속도도 다른 사료 대비 현저하게 단축시킴에 따라, 이는 본 발명의 흰점박이꽃무지 유충의 생장촉진용 인공사료가 흰점박이꽃무지의 연중 인공사육에 적합함을 알 수 있다. As the artificial feed for growth promotion of the white spotted flower larvae of the present invention prepared as described above is fed to the white spotted flower larvae, the survival rate is increased to 70% or more, and the growth rate is significantly reduced compared to other feeds. It can be seen that the artificial feed for promoting growth of the white spotted flower larvae of the present invention is suitable for artificial breeding of the white spotted flower plain.
이하에서는 실시예를 들어 본 발명에 관하여 더욱 상세하게 설명할 것이나, 이들 실시예는 단지 설명의 목적을 위한 것으로 본 발명의 보호 범위를 제한하고자 하는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to examples, but these examples are for illustrative purposes only and are not intended to limit the protection scope of the present invention.
<실시예 1,2 및 비교예 1,2,3> 흰점박이꽃무지 유충의 생장촉진용 인공사료 제조<Example 1,2 and Comparative Examples 1,2,3> Preparation of artificial feed for promoting growth of white spotted flower larvae
각각의 인공사료를 하기 표 1의 조성비율에 맞춰 혼합하여 제조하였다. Each artificial feed was prepared by mixing according to the composition ratio of Table 1 below.
이때, 인공사료의 재료로 톱밥(신우임산에서 구입), 카제인(대정화금에서 구입), 옥수수전분(제일제당에서 구입), 밀기울(우리밀 가공공장에서 구입)을 구입하여 적용하였다.At this time, sawdust (purchased from Shinwoo Imsan), casein (purchased from Daejung Geum), corn starch (purchased from CheilJedang), and wheat bran (purchased from Woori Wheat Processing Plant) were used as materials for artificial feed.
이렇게 제조된 실시예 1,2 및 비교예 1,2,3의 각 혼합물에 혼합물 100 중량부를 기준으로 40 중량부의 물을 넣어서 각각의 물질 들이 잘 섞일 수 있도록 각각 제조한 후, 온도 25±3℃ 저장고에 3개월동안 저장하여 최종 실시예 1,2 및 비교예 1,2,3의 흰점박이꽃무지 유충의 생장촉진용 인공사료들을 제조하였다.To each mixture of Examples 1, 2 and Comparative Examples 1, 2, and 3 prepared by adding 40 parts by weight of water based on 100 parts by weight of the mixture, each was prepared to be well mixed, and the temperature was 25±3°C. Stored for 3 months in the storage, artificial feeds for promoting growth of white spotted flower larvae of the final examples 1,2 and comparative examples 1,2 and 3 were prepared.
<실험예 1> 사료 발효기간에 따른 온도변화 측정<Experimental Example 1> Measurement of temperature change according to the fermentation period of feed
1. 실험방법1. Experimental method
상기 실시예 1,2 및 비교예 1 내지 3의 흰점박이꽃무지 유충의 생장촉진용 인공사료들을 대상으로 1주일 간격으로 사료의 온도변화를 측정하였다. The temperature changes of the feed were measured at weekly intervals for the artificial feeds for growth of white spotted flower larvae of Examples 1 and 2 and Comparative Examples 1 to 3.
2. 실험결과. 2. Experimental results.
상기 실험결과, 도 1에 나타나 있듯이, 사료발효시 최대 51℃까지 증가함을 확인할 수 있는 바, 상기 발효가 충분히 이루어지지 않은 상태에서 유충을 넣을 경우 유충이 폐사될 확률이 높음을 알 수 있었다. As shown in the experiment results, as shown in FIG. 1, it can be confirmed that the fermentation increases up to 51°C. When the fermentation is performed in a state in which the fermentation is not sufficiently performed, it is found that the probability of larvae death is high.
이에, 본 발명의 사료들은 2~3개월 동안 발효과정을 충분히 거친 후, 흰점박이 꽃무지 유충에 급이를 해야 생존율에 악영향을 미치지 않음을 알 수 있었다.Thus, it was found that the feeds of the present invention did not adversely affect the survival rate when the white spotted flower larvae were fed after a sufficient fermentation process for 2-3 months.
<실험예 2> 흰점박이꽃무지 유충의 섭식량 비교<Experimental Example 2> Comparison of feeding amount of white spotted flower larvae
1. 실험방법1. Experimental method
- 온도: 25℃ -Temperature: 25℃
- 대상: 흰점박이꽃무지 3령 유충 -Target: 3 spotted caterpillars
- 방법: 갓 탈피한 3령 유충의 무게를 측정하고, 각각의 인공사료를 넣어 11.4×8.2 cm 크기의 페트리디쉬에서 1마리씩 사육하며, 사료를 섭식한지 1주일이 지나 시기의 개체별 무게를 측정하고, 섭식하고 남은 똥의 무게를 측정하였다. -Method: Measure the weight of the freshly peeled three-star larvae, feed each artificial feed into 11.4×8.2 cm petri dishes, and measure the weight of each individual over a week after feeding. Then, the weight of the remaining shit after feeding was measured.
2. 실험결과2. Experimental results
상기 실험결과, 하기 표 2와 같이 나타났다. As a result of the experiment, it was shown in Table 2 below.
상기 표 2에 나타나 있듯이, 10일간 체증 증가를 보면 최소 0.157g에서 최대 1.044g으로 확인되었으며, T2 사료에서 가장 높은 체중 증가량을 보였다.As shown in Table 2, when the increase in congestion over 10 days was confirmed, the minimum was 0.157g and the maximum was 1.044g, and the highest weight gain was observed in the T2 feed.
또한, 사료의 섭식량은 최소 0.923g에서 최대 5.546g임을 확인하였으며, 사료 효율은 T2사료가 5.3 feed/gain으로 가장 효율적인 것을 확인하였다. In addition, it was confirmed that the feeding amount of the feed was from 0.923g to 5.546g, and the feed efficiency was confirmed to be the most efficient with T2 feed at 5.3 feed/gain.
<실험예 3> 흰점박이꽃무지의 사료에 따른 발육특성 분석<Experimental Example 3> Analysis of developmental characteristics of white spotted flower ignorance according to feed
1. 실험방법1. Experimental method
- 온도: 25℃ -Temperature: 25℃
- 대상: 흰점박이꽃무지 갓 부화한 1령 유충 -Target: 1-star larvae hatched freshly
- 방법: 각각의 인공사료를 넣어 11.4×8.2 cm 크기의 페트리디쉬에서 1마리씩 사육하며, 매일 생존유무와 탈피각 등을 확인하여 발육특성을 조사하였다. -Method: Each artificial feed was put in a 11.4×8.2 cm Petri dish, and each was bred, and the growth characteristics were examined by checking the existence of survival and peeling angle.
2. 실험결과2. Experimental results
상기 실험결과, 하기 표 3과 같이 나타났다. As a result of the experiment, it was shown in Table 3 below.
상기 표 3에 나타나 있듯이, T2, T3 사료에서 유충의 발육기간이 34.8일과 38.6일로 가장 짧게 나타나는 경향을 보였으며, 번데기 기간은 T4사료가 가장 낮은 27.3일로 나타나고, control구에서는 100일 이상이 지났음에도 불구하고, 번데기가 형성되지 않음을 확인하였다.As shown in Table 3, the larval development period of T2 and T3 feed was the shortest at 34.8 days and 38.6 days, and the pupal period was the lowest at T7.3 feed at 27.3 days, and even more than 100 days in the control group. Despite this, it was confirmed that the pupa was not formed.
<실험예 4> 흰점박이꽃무지의 사료에 따른 생존율 확인<Experimental Example 4> Confirmation of survival rate according to the feed of white spotted flower
1. 실험방법1. Experimental method
- 온도: 25℃ -Temperature: 25℃
- 대상: 흰점박이꽃무지 갓 부화한 1령 유충 -Target: 1-star larvae hatched freshly
- 방법: 각각의 인공사료를 넣어 11.4×8.2 cm크기의 페트리디쉬에서 1마리씩 사육하며, 매일 생존유무와 탈피각 등을 확인하여 발육특성을 조사하였다. -Method: Each artificial feed was put in a petri dish of 11.4×8.2 cm in size, and each of them was bred, and the growth characteristics were investigated by checking the existence of survival and peeling angle.
2. 실험결과2. Experimental results
상기 실험결과, 도 2에 나타나 있듯이, 성장이 빠른 T2, T3사료에서는 생존율이 70% 이상으로 나타났음을 확인하였다.As shown in the above experimental results, as shown in FIG. 2, it was confirmed that the survival rate was higher than 70% in the fast growing T2 and T3 feeds.
이상, 종합적 실험결과로 흰점박이꽃무지의 사료 개발을 위해서 카제인과, 옥수수 전분의 비율을 달리한 결과 카제인의 비율이 높은 T1 사료에서는 생존이 불가능하였고, T2와 T3 사료에서는 발육속도가 다른 사료에 비해 현저하게 빨라지는 것을 확인할 수 있었다. 또한 생존율도 70% 이상으로 높게 나타나고 있어, 앞으로 흰점박이꽃무지의 산업적 이용을 위해서 속성으로 사육이 가능함을 알 수 있다. As a result, as a result of the comprehensive experiments, as a result of varying the ratio of casein and corn starch for the development of feed for white-spotted flowers, it was impossible to survive in T1 feed with a high ratio of casein, and in T2 and T3 feed, the feed rate was different. It was confirmed that it was significantly faster than that. In addition, the survival rate is also high, which is 70% or higher, so it can be seen that it can be reared as an attribute for the industrial use of white spotted ignorance.
상기의 본 발명은 바람직한 실시예 및 실험예를 중심으로 살펴보았으며, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자는 본 발명의 본질적 기술 범위 내에서 상기 본 발명의 상세한 설명과 다른 형태의 실시예들을 구현할 수 있을 것이다. 여기서 본 발명의 본질적 기술범위는 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다. The present invention has been described with reference to preferred embodiments and experimental examples, and those of ordinary skill in the art to which the present invention pertains have a different form from the detailed description of the present invention within the essential technical scope of the present invention. You will be able to implement examples. Here, the essential technical scope of the present invention is indicated in the claims, and all differences within the equivalent range should be interpreted as being included in the present invention.
Claims (8)
흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물.
Containing a fermented product of a mixture consisting of sawdust, casein, corn starch, bran,
Artificial feed composition for promoting growth of white spotted flower larvae.
상기 혼합물은 상기 톱밥 60~80 중량%, 상기 카제인 10~25 중량%, 상기 옥수수전분 5~15 중량%, 상기 밀기울 1~5 중량%로 구성되는 것이 특징인,
흰점박이꽃무지 유충의 생장 촉진용 인공사료 조성물.
According to claim 1,
Characterized in that the mixture is composed of 60 to 80% by weight of the sawdust, 10 to 25% by weight of casein, 5 to 15% by weight of corn starch, and 1 to 5% by weight of bran,
Artificial feed composition for promoting growth of white spotted flower larvae.
상기 혼합물에 물을 넣은 후 발효하여 흰점박이꽃무지 유충의 생장 촉진용 인공사료를 제조하는 제2단계;를 포함하는,
흰점박이꽃무지 유충의 생장 촉진용 인공사료의 제조방법.
The first step of preparing a mixture of sawdust, casein, corn starch, wheat bran, and
A second step of preparing an artificial feed for promoting growth of white spotted flower larvae by adding water to the mixture and fermenting;
Method for manufacturing artificial feed for promoting growth of white spotted flower larvae.
상기 제1단계의 혼합물은 상기 톱밥 60~80 중량%, 상기 카제인 10~25 중량%, 상기 옥수수전분 5~15 중량%, 상기 밀기울 1~5 중량%로 구성되는 것이 특징인,
흰점박이꽃무지 유충의 생장 촉진용 인공사료의 제조방법.
According to claim 3,
The mixture of the first step is characterized in that it consists of 60 to 80% by weight of the sawdust, 10 to 25% by weight of casein, 5 to 15% by weight of cornstarch, and 1 to 5% by weight of bran,
Method for manufacturing artificial feed for promoting growth of white spotted flower larvae.
상기 제2단계의 인공사료 제조시, 상기 혼합물에 혼합물 100 중량부를 기준으로 40~50 중량부의 물을 넣는 것을 특징으로 하는,
흰점박이꽃무지 유충의 생장 촉진용 인공사료의 제조방법.
According to claim 3,
When preparing the artificial feed of the second step, characterized in that to add 40 to 50 parts by weight of water based on 100 parts by weight of the mixture,
Method for manufacturing artificial feed for promoting growth of white spotted flower larvae.
상기 제2단계의 인공사료 제조시, 상기 발효는 20~30℃에서 2~3개월동안 이루어지는 것을 특징으로 하는,
흰점박이꽃무지 유충의 생장 촉진용 인공사료의 제조방법.
According to claim 3,
When manufacturing the artificial feed of the second step, the fermentation is characterized in that made for 2 to 3 months at 20 ~ 30 ℃,
Method for manufacturing artificial feed for promoting growth of white spotted flower larvae.
흰점박이꽃무지 유충의 생장 촉진용 인공사료.
It is manufactured by the manufacturing method of any one of claims 3 to 6,
Artificial feed for promoting growth of white spotted flower larvae.
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