CN108094055A - A kind of cultural method of Termitomyces albuminosus with black skin solid layer frame - Google Patents
A kind of cultural method of Termitomyces albuminosus with black skin solid layer frame Download PDFInfo
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- CN108094055A CN108094055A CN201711227197.2A CN201711227197A CN108094055A CN 108094055 A CN108094055 A CN 108094055A CN 201711227197 A CN201711227197 A CN 201711227197A CN 108094055 A CN108094055 A CN 108094055A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
A kind of cultural method of Termitomyces albuminosus with black skin solid layer frame, including step：1）：Prepare bacterium bag, determine culture substrate raw material components and mass fraction proportioning, 40 50 parts of sawdust, 40 parts of DDS30,30 40 parts of cotton seed hulls, 24 parts of dregs of beans, 56 parts of corn flour, 23 parts of wheat bran, 34 parts of lime, 5 10 parts of termitarium soil, it is mixed with water, water content is 40 60%, and bacterium bag is made；2）：Cultural hypha, after sterilizing by the mycelia parent species block stripping and slicing containing termite secretion be 1.5 2cm3, it is put into culture medium, the constant temperature incubation under 21 ± 1 DEG C and dark conditions, culture shows physiological maturity in 15 20 days to mycelia annesl, you can into the cultivation stage；3）：Cultivation；4）：Harvesting treats that mushroom is grown to 5 8cm, fruiting harvesting.The incubation of the present invention is simple, success rate is high, at low cost and suitable for large-scale culture.
Description
Technical field
The invention belongs to edible mushroom culture technique fields, and in particular to a kind of culture side of Termitomyces albuminosus with black skin solid layer frame
Method.
Background technology
Termitomyces albuminosus with black skin is a kind of edible mushroom full of nutrition, it is rich in a variety of amino acid, is the edible mushroom of medicine-food two-purpose
One of, the effect of existing enriching the blood to restore normal menstruation, strengthening the spleen and stomach, and the symptoms such as loss of appetite, endless diarrhea can be treated, and have inhibition human body
The effect of growth of cancer cells.Although the nutritive value of Termitomyces albuminosus with black skin is very high, artificial culture is not easy, because casting skin chicken fir
Requirement of the bacterium to growing environment is extremely harsh, and collybia albuminosa itself will not be bred, and entire breeding and growth cycle will dwell by soil
Property termite, in long-term evolutionary process gradually and termite is into turning to the symbiosis ecosystem, the basidiospore of termite propagation collybia albuminosa
And mycelium, cultured mycelia, the collybia albuminosa mycelium for turning out also is taken as food, on the other hand, collybia albuminosa mycelium
Growth and development also be unable to do without termite secretion.For these reasons, the prior art not yet realizes the big rule of Termitomyces albuminosus with black skin
Mould is manually cultivated.
The content of the invention
Based on problem existing in the prior art, it is an object of the invention to provide a kind of incubation is simple, success rate is high,
The cultural method of Termitomyces albuminosus with black skin solid layer frame at low cost and suitable for large-scale culture, to solve in the prior art to casting skin chicken
The vacancy of fir bacterium artificial culture technique on a large scale meets the eating requirements of people.
In order to achieve the goal above, the technical solution adopted by the present invention is：
A kind of cultural method of Termitomyces albuminosus with black skin solid layer frame, the cultural method include the following steps：
Step 1）：Bacterium bag is prepared, determines culture substrate raw material components and mass fraction proportioning, 40-50 parts of sawdust, DDS30-40
Part, 30-40 parts of cotton seed hulls, 2-4 parts of dregs of beans, 5-6 parts of corn flour, 2-3 parts of wheat bran, 3-4 parts of lime, 5-10 parts of termitarium soil, add
Water is uniformly mixed, and bacterium bag is made in water content 40-60%；
Step 2）：Cultural hypha, after sterilizing by the mycelia parent species block stripping and slicing containing termite secretion be 1.5-2cm3, it is put into culture
In base, the constant temperature incubation under 21 ± 1 DEG C and dark conditions, culture shows physiological maturity in 15-20 days to mycelia annesl, you can into
Enter the cultivation stage；
Step 3）：Cultivation, by step 2）Cultured bacterium bag is uniformly distributed on stereoscopic multi-layer field planting frame disposed within, indoor screening
Light completes earthing 3-5cm after field planting, and growing temperatures are 15-20 DEG C, and day and night temperature is controlled in 8-13 DEG C, appropriate to water, and is kept
Native humidity is 65-70% above mycelia, is cultivated 30-35 days；
Step 4）：Harvesting treats that mushroom is grown to 5-8cm, fruiting harvesting.
Further, the culture substrate raw material components and mass fraction proportioning, 50 parts of sawdust, DDS35 parts, cotton seed hulls 35
Part, 8 parts of 3 parts of dregs of beans, 5 parts of corn flour, 3 parts of wheat bran, 3 parts of lime, termitarium soil, are mixed with water, water content 50%.
Further, the step 2）Middle sterilization process selects pressure as when high pressure sterilization 2.5-3 is small under 1.5kg/cm2.
Further, the stereoscopic multi-layer field planting frame is the supporting rack with multilayer nylon wire or wire netting.
The DDS (soluble alcohol grain) added in the incubation of the Termitomyces albuminosus with black skin of the present invention in culture substrate raw material
With termitarium soil, due to being rich in crude protein, crude fibre, available phosphorus, potassium, sodium and abundant amino acid in DDS, can effectively add
The speed of growth of fast mycelia, and DDS is generally acidity, is particularly conducive to the growth of collybia albuminosa mycelia.And contain in termitarium soil
40% or so termite secretion and excreta, therefore the effect of gas concentration lwevel in culture medium can be improved, and high concentration
Carbon dioxide be conducive to improve collybia albuminosa dense degree of mycelia, promote the growth of collybia albuminosa；Whole pedigree seed culture medium PH
Be worth for 4 .5 or so and gas concentration lwevel it is high, it is similar with the growing environment of wild collybia albuminosa, artificial cultivation collybia albuminosa can be improved
Yield and quality；And compare and be easier to gather with termite secretion, termitarium soil, suitable large-scale culture.
Compared with prior art, the invention has the advantages that：
DDS and the termitarium soil added in the incubation of the Termitomyces albuminosus with black skin of the present invention in culture substrate raw material, due to DDS
The speed of growth of mycelia can effectively be accelerated, and DDS is generally acidity, is particularly conducive to the growth of collybia albuminosa mycelia.It is and white
Containing 40% or so termite secretion and excreta in ant nest soil, it can also promote the growth of collybia albuminosa；And whole original seed
Culture medium pH value is 4.5 or so, similar with the growing environment of wild collybia albuminosa, can improve the yield and product of artificial cultivation collybia albuminosa
Matter.The present invention, convenient for scientific management and manual adjustment planting environment temperature and humidity etc., can be promoted by the way of three-dimensional layer frame
Make the stay in grade of Termitomyces albuminosus with black skin and uniformly, realize the extensive artificial cultivation of Termitomyces albuminosus with black skin, can both reduce field
The influence brought to wild environment is picked, and great market demand of the people to Termitomyces albuminosus with black skin can be met.
Specific embodiment
The cultural method of the Termitomyces albuminosus with black skin of the present invention is explained in detail with reference to specific examples below, but not as
Limitation of the invention.
Embodiment 1
A kind of cultural method of Termitomyces albuminosus with black skin solid layer frame, the cultural method include the following steps：
Step 1）：Bacterium bag is prepared, determines culture substrate raw material components and mass fraction proportioning, 50 parts of sawdust, DDS45 parts, cottonseed
35 parts of shell, 3 parts of dregs of beans, 5 parts of corn flour, 3 parts of wheat bran, 3 parts of lime, 8 parts of termitarium soil, are mixed with water, water content is
50%, bacterium bag is made；
Step 2）：Cultural hypha, after sterilizing by the mycelia parent species block stripping and slicing containing termite secretion be 1.5cm3, it is put into culture medium
In, the constant temperature incubation under 21 ± 1 DEG C and dark conditions cultivates 18 days to mycelia annesl physiological maturity, into the cultivation stage；
Step 3）：Cultivation, by step 2）Cultured bacterium bag is uniformly distributed on stereoscopic multi-layer field planting frame disposed within, indoor screening
Light completes earthing 4cm after field planting, and growing temperatures are 18 DEG C, and day and night temperature is kept in 8-10 DEG C, to water in right amount above mycelia
The humidity of soil is 65-68%, is cultivated 32 days；
Step 4）：Harvesting forms former base in bacterium bag after cultivating 32 days and grows very long rhizoid, and former base top expands to form mushroom
Flower bud emits unearthed face, and passes through the growth of 7 days or so, is harvested when mushroom is grown to 8-10cm, receives 4 batches of mushrooms, biological efficiency altogether
80%。
Embodiment 2
A kind of cultural method of Termitomyces albuminosus with black skin solid layer frame, the cultural method include the following steps：
Step 1）：Bacterium bag is prepared, determines culture substrate raw material components and mass fraction proportioning, 48 parts of sawdust, DDS36 parts, cottonseed
40 parts of shell, 4 parts of dregs of beans, 6 parts of corn flour, 3 parts of wheat bran, 3 parts of lime, 10 parts of termitarium soil, are mixed with water, water content is
50%, bacterium bag is made；
Step 2）：Cultural hypha, after sterilizing by the mycelia parent species block stripping and slicing containing termite secretion be 2cm3, it is put into culture medium
In, the constant temperature incubation under 21 ± 1 DEG C and dark conditions cultivates 20 days to mycelia annesl physiological maturity, into the cultivation stage；
Step 3）：Cultivation, by step 2）Cultured bacterium bag is uniformly distributed on stereoscopic multi-layer field planting frame disposed within, indoor screening
Light completes earthing 5cm after field planting, and growing temperatures are 20 DEG C, and day and night temperature is kept in 8-10 DEG C, to water in right amount above mycelia
The humidity of soil is 65-68%, is cultivated 30 days；
Step 4）：Harvesting forms former base in bacterium bag after cultivating 30 days and grows very long rhizoid, and former base top expands to form mushroom
Flower bud emits unearthed face, and passes through the growth of 7 days or so, is harvested when mushroom is grown to 8-10cm, receives 4 batches of mushrooms, biological efficiency altogether
70%。
Embodiment 3
A kind of cultural method of Termitomyces albuminosus with black skin solid layer frame, the cultural method include the following steps：
Step 1）：Bacterium bag is prepared, determines culture substrate raw material components and mass fraction proportioning, 45 parts of sawdust, DDS32 parts, cottonseed
38 parts of shell, 4 parts of dregs of beans, 6 parts of corn flour, 3 parts of wheat bran, 4 parts of lime, 8 parts of termitarium soil, are mixed with water, water content is
55%, bacterium bag is made；
Step 2）：Cultural hypha, after sterilizing by the mycelia parent species block stripping and slicing containing termite secretion be 2cm3, it is put into culture medium
In, the constant temperature incubation under 21 ± 1 DEG C and dark conditions cultivates 20 days to mycelia annesl physiological maturity, into the cultivation stage；
Step 3）：Cultivation, by step 2）Cultured bacterium bag is uniformly distributed on stereoscopic multi-layer field planting frame disposed within, indoor screening
Light completes earthing 4cm after field planting, and growing temperatures are 20 DEG C, and day and night temperature is kept in 8-10 DEG C, to water in right amount above mycelia
The humidity of soil is 68-70%, is cultivated 35 days；
Step 4）：Harvesting forms former base in bacterium bag after cultivating 35 days and grows very long rhizoid, and former base top expands to form mushroom
Flower bud emits unearthed face, and passes through the growth of 7 days or so, is harvested when mushroom is grown to 8-10cm, receives 4 batches of mushrooms, biological efficiency altogether
75%。
By the implantation methods of Termitomyces albuminosus with black skin in above-described embodiment, the biological efficiency of the Termitomyces albuminosus with black skin harvested is equal
Higher than 70%, it was demonstrated that by implantation methods provided by the invention, the yield of Termitomyces albuminosus with black skin can be improved, and given birth to suitable for extensive
Production.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, still may be used
To modify to the technical solution recorded in foregoing embodiments or carry out equivalent substitution to which part technical characteristic.
Within the spirit and principles of the invention, any modifications, equivalent replacements and improvements are made should be included in the present invention's
Within protection domain.
Claims (4)
1. a kind of cultural method of Termitomyces albuminosus with black skin solid layer frame, which is characterized in that the cultural method includes the following steps：
Step 1）：Bacterium bag is prepared, determines culture substrate raw material components and mass fraction proportioning, 40-50 parts of sawdust, DDS30-40
Part, 30-40 parts of cotton seed hulls, 2-4 parts of dregs of beans, 5-6 parts of corn flour, 2-3 parts of wheat bran, 3-4 parts of lime, 5-10 parts of termitarium soil, add
Water is uniformly mixed, and bacterium bag is made in water content 40-60%；
Step 2）：Cultural hypha, after sterilizing by the mycelia parent species block stripping and slicing containing termite secretion be 1.5-2cm3, it is put into culture
In base, the constant temperature incubation under 21 ± 1 DEG C and dark conditions, culture shows physiological maturity in 15-20 days to mycelia annesl, you can into
Enter the cultivation stage；
Step 3）：Cultivation, by step 2）Cultured bacterium bag is uniformly distributed on stereoscopic multi-layer field planting frame disposed within, indoor screening
Light completes earthing 3-5cm after field planting, and growing temperatures are 15-20 DEG C, and day and night temperature is controlled in 8-13 DEG C, appropriate to water, and is kept
Native humidity is 65-70% above mycelia, is cultivated 30-35 days；
Step 4）：Harvesting treats that mushroom is grown to 5-8cm, fruiting harvesting.
2. the cultural method of Termitomyces albuminosus with black skin solid layer frame according to claim 1, which is characterized in that the culture substrate
Raw material components and mass fraction proportioning, 50 parts of sawdust, DDS35 parts, 35 parts of cotton seed hulls, 3 parts of dregs of beans, 5 parts of corn flour, 3 parts of wheat bran,
3 parts of lime, 8 parts of termitarium soil, are mixed with water, water content 50%.
3. the cultural method of Termitomyces albuminosus with black skin solid layer frame according to claim 1, which is characterized in that the step 2）In
Sterilization process selects pressure as 1.5kg/cm2When lower high pressure sterilization 2.5-3 is small.
4. the cultural method of Termitomyces albuminosus with black skin solid layer frame according to claim 1, which is characterized in that the stereoscopic multi-layer
Field planting frame is the supporting rack with multilayer nylon wire or wire netting.
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| CN201711227197.2A CN108094055A (en) | 2017-11-29 | 2017-11-29 | A kind of cultural method of Termitomyces albuminosus with black skin solid layer frame |
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| CN201711227197.2A CN108094055A (en) | 2017-11-29 | 2017-11-29 | A kind of cultural method of Termitomyces albuminosus with black skin solid layer frame |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111213547A (en) * | 2018-11-26 | 2020-06-02 | 深圳市鼎宸生物科技有限公司 | Cultivation method of new black-skin termitomyces albuminosus |
| CN112042463A (en) * | 2020-08-06 | 2020-12-08 | 贵州丰源现代农业有限公司 | Black termitomyces albuminosus culture method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
| CN105474995A (en) * | 2015-12-21 | 2016-04-13 | 镇江盛弘景观植物有限公司 | Cultivation and domestication method of wild collybia albuminosa |
| CN106892749A (en) * | 2017-02-27 | 2017-06-27 | 金乡县联盛菌业科技有限公司 | A kind of method of Termitomyces albuminosus with black skin solid layer frame plantation |
-
2017
- 2017-11-29 CN CN201711227197.2A patent/CN108094055A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
| CN105474995A (en) * | 2015-12-21 | 2016-04-13 | 镇江盛弘景观植物有限公司 | Cultivation and domestication method of wild collybia albuminosa |
| CN106892749A (en) * | 2017-02-27 | 2017-06-27 | 金乡县联盛菌业科技有限公司 | A kind of method of Termitomyces albuminosus with black skin solid layer frame plantation |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111213547A (en) * | 2018-11-26 | 2020-06-02 | 深圳市鼎宸生物科技有限公司 | Cultivation method of new black-skin termitomyces albuminosus |
| CN112042463A (en) * | 2020-08-06 | 2020-12-08 | 贵州丰源现代农业有限公司 | Black termitomyces albuminosus culture method and application thereof |
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