CN103333011A - Compatibility of culture material of coprinus comatus cultivation seeds and preparation method of culture material - Google Patents
Compatibility of culture material of coprinus comatus cultivation seeds and preparation method of culture material Download PDFInfo
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- CN103333011A CN103333011A CN2013102875408A CN201310287540A CN103333011A CN 103333011 A CN103333011 A CN 103333011A CN 2013102875408 A CN2013102875408 A CN 2013102875408A CN 201310287540 A CN201310287540 A CN 201310287540A CN 103333011 A CN103333011 A CN 103333011A
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- culture material
- silkworm excrement
- coprinus comatus
- cultivation seeds
- chicken leg
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a culture material of coprinus comatus cultivation seeds. The coprinus comatus culture material is characterized by taking excrement of 5th instar bombyx mori as a raw material, and adding mushroom residues of coprinus comatus as a bridging material used among particles so as to prepare the coprinus comatus cultivation seeds. The water content of the excrement of 5th instar bombyx mori is 45%, and the water content of the bridging material is 65%. The invention also discloses a preparation method of the culture material. Compared with an existing wheat culture material, advantages of the culture material disclosed in the invention are that: (1) the nutritive value of silkworm excrement is comprehensive, so that the growth of mycelia is vigorous, the vitality of mycelia is strong, and the aging of mycelia is difficult to happen; (2) the cultivation seeds possess the same shape of wheat cultivation seeds, so that the cultivation seeds are easy to disperse during seeding; (3) pathogens of silkworm excrement are killed in a disinfection process of the cultivation seeds preparation, so that the environment of bombyx mori culture areas is improved; and (4) wheat is replaced by silkworm excrement, so that grain and forage are saved, the sources of raw materials are increased, and the cost of cultivation seeds is reduced.
Description
Technical field
Content of the present invention comprises cultivating chicken leg mushroom kind culture material compatibility, and the making method of this culture material, belongs to edible fungus species and makes the field.
Background technology
Coprinus comatus is a kind of universal rare edible mushrooms, the likeness in form chicken leg, and the crisp cunning of meat, delicious flavour, nutritious.According to surveying and determination, contain crude protein 25.4g in the dried mushroom of every 100g, fatty 3.3g, total reducing sugar 58.8g also contains multiple amino acids in the Coprinus comatus, comprise the amino acid of 8 kinds of needed by human.Coprinus comatus is again a kind of medicinal fungi, has functions such as aid digestion, strengthening the spleen and stomach, raising body immunity, is subjected to domestic and international human consumer's favor deeply.
Along with the continuous expansion of cultivating chicken leg mushroom area, the wheat consumption that the production of hybrid seeds is used also increases thereupon, for reducing grain consumption, must seek the reasonable substitute of the production of hybrid seeds.
There is long sericulture history in China, also is maximum in the world sericulture state, and silk cocoon production occupies world's monopoly position.Silkworm excrement is the ight soil of silkworm, is the by product of sericulture, and the annual above dry of 400kt of can collecting approximately is a rich in natural resources.
The silkworm excrement comprehensive nutrition, dried silkworm excrement contains crude protein 13.4%-14.5%, crude fat 2.2%-2.3%, robust fibre 15.8%-16.2%, nitrogen-free extract 56.9%-57.4%, trace elements such as cupric, iron and zinc also, and contain plant growth hormoness such as a small amount of alkaloid, class adrenocortical hormone, Va, Vb, Vc, Vd, Ve, nicotinic acid, indolylacetic acid and triacontanol price quote.
Coprinus comatus can utilize organic carbon source and the organic nitrogen compound in the silkworm excrement, mainly is Mierocrystalline cellulose, protein etc., and this is the most basic nutritive substance of Coprinus comatus growth.The promoter action that nitrogen in the silkworm excrement, phosphorus, potassium, trace element and growth stimulant are certain to growing of shaggy mane mycelium body.
Five ages, dried silkworm excrement was as the mung bean size, and particle diameter is between 2mm-4mm, and diameter is between 5mm-6mm after the water-swelling.If with silkworm excrement making in five ages cultivating chicken leg mushroom kind, not only the bacterial classification cost descends, and has the particle shape of kernel culture, the advantage that easily scatter sowing time, makes silkworm excrement obtain innoxious use simultaneously.
Summary of the invention
The present invention has following two aspect contents:
1, a kind of cultivating chicken leg mushroom kind culture material compatibility
2, the making method of culture material
The technical solution adopted in the present invention is:
A kind of cultivating chicken leg mushroom kind culture material compatibility has following component and quality proportioning: silkworm excrement grain 84%-88%, and Coprinus comatus bacterium chaff 8%-12%, terra alba 3%, sucrose 1% all is the quality of dry-matter, the proportioning sum is 100%.
The silkworm excrement grain: fresh nothing go mouldy five age silkworm excrement dry and sieve, it is standby to remove the residual leaf of mulberry and other foreign material.Nitrogenous (N) 2.97% of dried silkworm excrement, phosphorus (P
2O
5) 1.03%, potassium (K
2O) 3.52%.
Coprinus comatus bacterium chaff: be the leftover bits and pieces bacterium material behind the cultivating chicken leg mushroom, with its particle that is ground into particle diameter 1mm-2mm, dry standby.
Terra alba: be common commercially available product.Can play and stablize the culture material potential of hydrogen, increase the effect of calcium, sulphur nutritive ingredient.
Sucrose: be that Coprinus comatus is cultivated replenishing of initial stage carbon source, can induce the generation of extracellular enzyme simultaneously, accelerate coarse-fibred utilization.
The making method of culture material comprises the steps:
1. soak: dried silkworm excrement soaks 15min-20min with clear water, and soft slightly and cannot not pinch mashedly degree of being with silkworm excrement, water content was 45% (being puffy);
2. spice: bacterium chaff, terra alba siccative are mixed thoroughly, and the sucrose water spice with 1% makes the water content of bacterium chaff reach 65%, then stirs with the silkworm excrement that had soaked;
3. bottling: with the silkworm excrement culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
4. sterilize, cool off: culturing bottle is carried out autoclaving, make 100 ℃ of sterilization 1h earlier, make 121 ℃ of autoclaving 1.5h then; The type of cooling is: naturally cool to below 25 ℃.
Culture material is after inserting original seed, and culturing room's temperature control is at 22 ℃-24 ℃, and relative air humidity is controlled at 60%-75%, and through the cultivation of 22d-23d, mycelia is covered with bottle, and 3d is cultivated in after-ripening then, just can be used for producing.
The present invention is raw material with silkworm silkworm excrement in five ages, admixes Coprinus comatus bacterium chaff as intergranular bridging material, makes the cultivating chicken leg mushroom kind.Compare with the wheat kind, its advantage is: the one, and the silkworm excrement comprehensive nutrition, mycelial growth is vigorous, and spawn activity is strong, is difficult for aging; The 2nd, the bacterial classification of preparation has the particle shape of kernel culture, and easily scatter sowing time; The 3rd, the sterilization link of the production of hybrid seeds has been killed pathogenic bacteria in the silkworm excrement, has purified the hygienic condition in silkworm district; The 4th, replace wheat with silkworm excrement, saved grain and feed, widened the raw material channel, reduced the bacterial classification cost.
Embodiment
Mode 1: cultivating chicken leg mushroom kind culture material comprises following component and quality proportioning in the present embodiment: silkworm excrement grain 84%, and Coprinus comatus bacterium chaff 12%, terra alba 3%, sucrose 1% all is the quality of dry-matter, the proportioning sum is 100%.
Making method:
1. soak: dried silkworm excrement soaks 17min with clear water, and soft slightly and cannot not pinch mashedly degree of being with silkworm excrement, water content was 45% (being puffy);
2. spice: bacterium chaff, terra alba siccative are mixed thoroughly, and the sucrose water spice with 1% makes the water content of bacterium chaff reach 65%, then stirs with the silkworm excrement that had soaked;
3. bottling: with the silkworm excrement culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
4. sterilize, cool off: culturing bottle is carried out autoclaving, make 100 ℃ of sterilization 1h earlier, make 121 ℃ of autoclaving 1.5h then; The type of cooling is: naturally cool to below 25 ℃.
Culture material is after inserting original seed, and culturing room's temperature control is at 22 ℃, and relative air humidity is controlled 65%, and through the cultivation of 22d, mycelia is covered with bottle, and 3d is cultivated in after-ripening then, just can be used for producing.
Mode 2: cultivating chicken leg mushroom kind culture material comprises following component and quality proportioning in the present embodiment: silkworm excrement grain 88%, and Coprinus comatus bacterium chaff 8%, terra alba 3%, sucrose 1% all is the quality of dry-matter, the proportioning sum is 100%.Making method is with reference to mode 1.
Mode 3 (control group): Coprinus comatus wheat kind culture material: wheat 97%, terra alba 3%.
By following table as can be known, the sprout time of three kinds of mode Coprinus comatus mattress silks is 16h, and mycelial growth is dense, and the full bottle of silkworm excrement culture material time ratio control group shortens 5d-6d; Observe the aging index (mycelia color, yellow water amount) of bacterial classification, the silkworm excrement culture material of all modes is when sending out bacterium 60d, and mycelia is still pure white, no yellow water, and the mycelia of kernel culture is pale yellow, and a small amount of yellow water is arranged.
Table three group culture material is to the influence of cultivating chicken leg mushroom kind mycelial growth
Annotate: mycelial density with ++, +++and ++ ++ expression ,+number more multilist shows that mycelial density is more high.
Claims (4)
1. cultivating chicken leg mushroom kind culture material compatibility is characterized in that containing following component and quality proportioning: silkworm excrement grain 84%-88%, and Coprinus comatus bacterium chaff 8%-12%, terra alba 3%, sucrose 1% all is the quality of dry-matter, the proportioning sum is 100%.
2. cultivating chicken leg mushroom kind culture material according to claim 1, it is characterized in that described silkworm excrement grain be fresh nothing go mouldy five age silkworm excrement, dry and sieve, remove the residual leaf of mulberry and other foreign material.
3. cultivating chicken leg mushroom kind culture material according to claim 1 is characterized in that described Coprinus comatus bacterium chaff, is the leftover bits and pieces bacterium material behind the cultivating chicken leg mushroom, and it is ground into the particle of 1mm-2mm.
4. the making method of the cultivating chicken leg mushroom kind culture material described in the claim 1-3 is characterized in that:
1. soak: dried silkworm excrement soaks 15min-20min with clear water, and soft slightly and cannot not pinch mashedly degree of being with silkworm excrement, water content was 45% (being puffy);
2. spice: bacterium chaff, terra alba siccative are mixed thoroughly, and the sucrose water spice with 1% makes the water content of bacterium chaff reach 65%, and then the silkworm excrement that soaked with oneself stirs;
3. bottling: with the silkworm excrement culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
4. sterilize, cool off: culturing bottle is carried out autoclaving, make 100 ℃ of sterilization 1h earlier, make 121 ℃ of autoclaving 1.5h then; The type of cooling is: naturally cool to below 25 ℃.
Coprinus comatus culture material described in the 5 usefulness claim 1-4 is made cultivar, after it is characterized in that inserting original seed, culturing room's temperature control is at 22 ℃-24 ℃, relative air humidity control is at 60%-75%, cultivation through 22d-23d, mycelia is covered with bottle, and 3d is cultivated in after-ripening then, just can be used for producing.
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| CN201310287540.8A CN103333011B (en) | 2013-06-29 | 2013-06-29 | Compatibility of culture material of coprinus comatus cultivation seeds and preparation method of culture material |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104016735A (en) * | 2014-05-23 | 2014-09-03 | 广西地源之本肥业有限公司 | Fertilizer additive used for culturing coprinus comatus and preparation method thereof |
| CN104649746A (en) * | 2014-06-26 | 2015-05-27 | 黄小莲 | Fertilizer additive for culture of coprinus comatus and preparation method thereof |
| CN104987161A (en) * | 2015-08-11 | 2015-10-21 | 芜湖野树林生物科技有限公司 | Coprinus comatus culture medium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102613004A (en) * | 2012-04-14 | 2012-08-01 | 河北荣珍食用菌股份有限公司 | Method using pleurotus nebrodensis waste to cultivate flammulina velutipes |
| CN102807441A (en) * | 2012-08-06 | 2012-12-05 | 宁波市天禾农业发展有限公司 | Flammulina velutipes culture medium and preparation method and application thereof |
| CN103073351A (en) * | 2013-01-24 | 2013-05-01 | 詹天际 | Cultivation substrate of edible mushroom, preparation method of cultivation substrate and cultivation method of edible mushroom |
-
2013
- 2013-06-29 CN CN201310287540.8A patent/CN103333011B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613004A (en) * | 2012-04-14 | 2012-08-01 | 河北荣珍食用菌股份有限公司 | Method using pleurotus nebrodensis waste to cultivate flammulina velutipes |
| CN102807441A (en) * | 2012-08-06 | 2012-12-05 | 宁波市天禾农业发展有限公司 | Flammulina velutipes culture medium and preparation method and application thereof |
| CN103073351A (en) * | 2013-01-24 | 2013-05-01 | 詹天际 | Cultivation substrate of edible mushroom, preparation method of cultivation substrate and cultivation method of edible mushroom |
Non-Patent Citations (1)
| Title |
|---|
| 俞小华等: "利用桑枝蚕粪栽培食用菌(续一)", 《蚕桑通报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104016735A (en) * | 2014-05-23 | 2014-09-03 | 广西地源之本肥业有限公司 | Fertilizer additive used for culturing coprinus comatus and preparation method thereof |
| CN104649746A (en) * | 2014-06-26 | 2015-05-27 | 黄小莲 | Fertilizer additive for culture of coprinus comatus and preparation method thereof |
| CN104987161A (en) * | 2015-08-11 | 2015-10-21 | 芜湖野树林生物科技有限公司 | Coprinus comatus culture medium |
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| CN103333011B (en) | 2015-03-25 |
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