CN108293592A - A method of cultivating needle mushroom using sorghum flour mixture - Google Patents
A method of cultivating needle mushroom using sorghum flour mixture Download PDFInfo
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- CN108293592A CN108293592A CN201710964022.3A CN201710964022A CN108293592A CN 108293592 A CN108293592 A CN 108293592A CN 201710964022 A CN201710964022 A CN 201710964022A CN 108293592 A CN108293592 A CN 108293592A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
A method of cultivating needle mushroom using sorghum flour mixture, by raw material corncob, cotton seed hulls, wheat bran, brewex's grains, soybean skin, megasse, rice bran, maize flour, sorghum flour, shellfish fossil and calcium carbonate, it is added in agitated kettle after elder generation's dry mixing after wet-mixing, obtain mixture, it is packed into flammulina velatipes culture bottle to shoulder, after being punched by puncher, it is put into autoclave and sterilizes；It is inoculated with after sterilizing, it is transferred to culturing room, mycelia covers with culture bottle, mycelia is carried out mechanical mycelium stimulation, the culture bottle after mycelium stimulation enters fertility room, mycelia restores kink, after forming former base, sleeve when growing to 17 days, using growth in 10 days 8 days, when mushroom body is grown to a little higher than sleeve piece, harvested.Advantage is：The raw material of Enoki mushroom cultivation material is easy to get, of low cost, can meet the nutritional need in Growth of Flammulina Velutipes each stage, while ensureing to stablize fruiting, improves the quality of needle mushroom.
Description
Technical field
The present invention relates to a kind of methods for cultivating needle mushroom using sorghum flour mixture.
Background technology
Needle mushroom [Flammuluina velutipes (Fr.V.) Sing.] is a kind of famous edible and medicinal fungi, because of it
Mushroom body colour pool is in golden yellow, mushroom handle is elongated like day lily, thus needle mushroom of gaining the name.Needle mushroom collection " natural, nutrition, health care " is in one
Body, the moisture about 82%-89% of fresh needle mushroom, rich in a large amount of protein, carbohydrate, mineral element, vitamin
And crude fibre, and fat content is relatively low, is a kind of rare high nutrition good merchantable brand.Contain 18 kinds of amino acid in needle mushroom, often
20.9g containing amino acid in the dry mushrooms of 100g, wherein accounting for the 42.29%- of total amino acid content containing 8 kinds of amino acid needed by human
51.17%, lysine and arginine content are extremely abundant, reach 1.024g and 1.231g respectively, all higher than general edible mushroom, do
1.3-6.8ug/g containing zinc in needle mushroom, and zinc is exactly the essential mineral matter element of upgrowth and development of children, can promote children
Healthy growth and intelligence development, therefore Japan be referred to as increase intelligence mushroom.Now, needle mushroom has become the master of China's artificial cultivation
One of edible mushroom is wanted, 70% or more of edible mushroom total output has been accounted for.
Currently, in the factorial praluction of needle mushroom, it is real that Enoki mushroom cultivation material selection can consider production cost, benefit etc.
Border situation is typically chosen industrial and agricultural production leftover bits and pieces weed tree sawdust, Cereal rich in cellulose, lignin, hemicellulose, carbohydrate
In rice straw, cotton seed hulls, bagasse, rapeseed slag, megasse, banana stem leaves, soybean rice straw, vinasse, vinegar grain, paper pulp slag, part
Dregs of a decoction etc. carry out the cultivation work of needle mushroom as carbon source.Since 2010, weed tree sawdust, cotton seed hulls that Edible Fungi uses etc.
Main raw material(s) price sharp rises, and the source of goods is in short supply, and the difficult steep increasing of Edible Fungi enterprise production, cost of material is caused to increase,
Seriously constrain the mushroom industrialized production of acupuncture needle.
Invention content
The technical problem to be solved in the present invention is to provide a kind of method for cultivating needle mushroom using sorghum flour mixture, acupuncture needles
The raw material of mushroom culture material is easy to get, of low cost, can meet the nutritional need in Growth of Flammulina Velutipes each stage, is ensureing to stablize fruiting
Meanwhile improving the quality of needle mushroom.
Technical solution of the invention is：
A method of needle mushroom being cultivated using sorghum flour mixture, is as follows:
(1) Enoki mushroom cultivation material dispensing
Percentage composition by weight, by raw material corncob 37%, cotton seed hulls 5%, wheat bran 5%, brewex's grains 5%, soybean skin
5%, megasse 4%, rice bran 26.5%, maize flour 5%, sorghum flour 5%, shellfish fossil 1.5% and calcium carbonate 1% are added to and stir
It mixes in pot, first time stirring is carried out in agitated kettle, then dry mixing 20min is carried out second with ensureing that each component is uniformly mixed
Stirring, wet-mixing 45min add water into raw material during wet-mixing, ensure that feed moisture content is 66.9%, obtain mixture；It will mix
It closes material to be sent to bottling machine by conveyer belt, mixture is packed into flammulina velatipes culture bottle to shoulder, it will be in culture bottle by puncher
Mixture is made a call to five holes after flattening and is covered, and is put into autoclave and sterilizes, sterilized by the way of high temperature and pressure, was entirely sterilized
Sterilization pressure >=0.15MPa in journey, sterilizing program is 105 DEG C and keeps the temperature 60 minutes, and 122 DEG C sterilize 60 minutes, keep the temperature 10 minutes, complete
At sterilizing, automatic exhaust 1 hour is cooling；
(2) it is inoculated with
The culture bottle equipped with mixture is inoculated with after sterilizing, it is desirable that temperature is 16 DEG C -18 DEG C in bottle, and inoculum concentration is
38mL-42mL enters via conveyer belt by culture bottle culturing room；
(3) culturing room cultivates
Culturing room requires indoor clean, heat preservation, well-ventilated, need not illuminate, it is desirable that the shoulder temperature of flammulina velatipes culture bottle
Degree is 17 DEG C -19.5 DEG C；It was cultivated to the 6th day just after inoculation, culture humidity is 70%, CO2Concentration is less than
2000ppm；It is cultivated in rear training within the 7th day to 23 days after inoculation, culture humidity is 75%-80%, CO2Concentration should control
2000ppm-3500ppm, through -23 days 22 days, mycelia covered with culture bottle；
(4) mycelium stimulation
Mycelia is subjected to mechanical mycelium stimulation；
(5) fertility room growth
Culture bottle after mycelium stimulation enters fertility room, and mycelia restores kink, after forming former base, sleeve when growing to 17 days, then
It is harvested when mushroom body is grown to a little higher than sleeve piece by growth in -10 days 8 days.
Further, the specification of the golden mushroom plantation bottle is
Further, mixture bottling amount is 1000g-1020g.
Further, when punching, it is desirable that the smooth nothing of charge level collapses, five hole of culture bottle bottom can light transmission, convenient for inoculation when
Strain can reach culture bottle bottom.
Further, sleeve piece height 15.5cm, mushroom body is higher by sleeve piece 1cm when harvesting.
The beneficial effects of the invention are as follows：
Using the leftover bits and pieces of the agricultural and sideline products such as corncob, cotton seed hulls as raw material, while being added to sorghum flour, maize flour mixing cultivation
Needle mushroom is trained, mycelia growth rate is fast, and fruit-body formation is early, and biological transformation ratio is high.Full of nutrition, the suitable acupuncture needle of sorghum
Mushroom grows, and sorghum is universal in north, and yield is high, and raw material is easy to get, and price is relatively reasonable, cultivates the biologicak efficiency of needle mushroom
100% can be reached, the waste material after fruiting can be used as the high-quality feed of livestock and poultry, increase additional income.
Cultural method is simple, scientific and reasonable, while ensure that stable fruiting, also improves the quality of needle mushroom.22 days-
The culture for completing mycelia in 22 days harvests for -27 days 25 days, and the entire production cycle only needs 60 days or so, and each culture bottle averagely produces mushroom
About 500 grams, economic benefit is largely improved by original per unit area yield 470g, every bottle of output increased 30g.
Description of the drawings
Fig. 1 is Growth of Flammulina Velutipes state diagram when needle mushroom harvests.
Specific implementation mode
Embodiment 1
(1) Enoki mushroom cultivation material dispensing
By raw material corncob 37kg, cotton seed hulls 5kg, wheat bran 5kg, brewex's grains 5kg, soybean skin 5kg, megasse 4kg, rice bran
26.5kg, maize flour 5kg, sorghum flour 5kg, shellfish fossil 1.5kg and calcium carbonate 1kg, are added in agitated kettle, in agitated kettle into
Row stirs for the first time, dry mixing 20min, to ensure that each component is uniformly mixed, then carries out stirring for second, wet-mixing 45min, wet-mixing
It is 66.9wt% to add water, guarantee feed moisture content into raw material in the process, obtains mixture；Mixture is sent by conveyer belt to dress
Bottle machine, mixture is packed intoFlammulina velatipes culture bottle in, charge 1000g；By puncher by culture bottle
Interior mixture is made a call to five holes after flattening and is covered, it is desirable that the smooth nothing of charge level collapses, five hole of culture bottle bottom can light transmission, convenient for connecing
Strain can reach culture bottle bottom when kind；It is put into autoclave to sterilize, be sterilized by the way of high temperature and pressure, entirely sterilized
Sterilization pressure >=0.15MPa in journey, sterilizing program is 105 DEG C and keeps the temperature 60 minutes, and 122 DEG C sterilize 60 minutes, keep the temperature 10 minutes, complete
At sterilizing, automatic exhaust 1 hour is cooling；
(2) it is inoculated with
The culture bottle equipped with mixture is inoculated with after sterilizing, it is desirable that and temperature is 16 DEG C, inoculum concentration 42mL in bottle, via
Conveyer belt enters culture bottle culturing room；
(3) culturing room cultivates
Culturing room requires indoor clean, heat preservation, well-ventilated, need not illuminate, it is desirable that the shoulder temperature of flammulina velatipes culture bottle
Degree is 17 DEG C；It was cultivated to the 6th day just after inoculation, culture humidity is 70%, CO2Concentration is less than 2000ppm；After inoculation
It is cultivated in rear training within 7th day to 23 days, culture humidity is 80%, CO2Concentration should be controlled in 2000ppm, and through 23 days, mycelia was long
Full culture bottle；
(4) mycelium stimulation
Mycelia is subjected to mechanical mycelium stimulation；
(5) fertility room growth
Culture bottle after mycelium stimulation enters fertility room, and mycelia restores kink, after forming former base, sleeve when growing to 17 days, and set
Cylinder piece height 15.5cm, using growth in 8 days, mushroom body was higher by sleeve piece 1cm, is harvested.Single bottle yield is 502g ± 3g.
Embodiment 2
(1) Enoki mushroom cultivation material dispensing
By raw material corncob 37kg, cotton seed hulls 5kg, wheat bran 5kg, brewex's grains 5kg, soybean skin 5kg, megasse 4kg, rice bran
26.5kg, maize flour 5kg, sorghum flour 5kg, shellfish fossil 1.5kg and calcium carbonate 1kg, are added in agitated kettle, in agitated kettle into
Row stirs for the first time, dry mixing 20min, to ensure that each component is uniformly mixed, then carries out stirring for second, wet-mixing 45min, wet-mixing
Add water into raw material in the process, ensures that feed moisture content is 66.9%, obtain mixture；Mixture is sent by conveyer belt to bottling
Machine, mixture, which is packed into specification, isFlammulina velatipes culture bottle in shoulder, mixture bottling amount is 1020g；
Five holes are made a call to after being flattened the mixture in culture bottle by puncher and are covered, and are put into autoclave and are sterilized, using high temperature and pressure
Mode sterilize, sterilization pressure >=0.15MPa in entire sterilization process, sterilizing program be 105 DEG C keep the temperature 60 minutes, 122 DEG C go out
Bacterium 60 minutes keeps the temperature 10 minutes, completes sterilizing, and automatic exhaust 1 hour is cooling；
(2) it is inoculated with
The culture bottle equipped with mixture is inoculated with after sterilizing, it is desirable that and temperature is 18 DEG C, inoculum concentration 38mL in bottle, via
Conveyer belt enters culture bottle culturing room；
(3) culturing room cultivates
Culturing room requires indoor clean, heat preservation, well-ventilated, need not illuminate, it is desirable that the shoulder temperature of flammulina velatipes culture bottle
Degree is 19.5 DEG C；It was cultivated to the 6th day just after inoculation, culture humidity is 70%, CO2Concentration is less than 2000ppm；Inoculation
It is cultivated afterwards in rear training within the 7th day to 23 days, culture humidity is 75%, CO2Concentration should be controlled in 3500ppm, through 22 days, mycelia
Cover with culture bottle；
(4) mycelium stimulation
Mycelia is subjected to mechanical mycelium stimulation；
(5) fertility room growth
Culture bottle after mycelium stimulation enters fertility room, and mycelia restores kink, after forming former base, sleeve when growing to 17 days, and set
Cylinder piece height 15.5cm, using growth in 10 days, mushroom body was higher by sleeve piece 1cm, is harvested.Single bottle yield be 505g ±
5g。
Embodiment 3
(1) Enoki mushroom cultivation material dispensing
By raw material corncob 37kg, cotton seed hulls 5kg, wheat bran 5kg, brewex's grains 5kg, soybean skin 5kg, megasse 4kg, rice bran
26.5kg, maize flour 5kg, sorghum flour 5kg, shellfish fossil 1.5kg and calcium carbonate 1kg, are added in agitated kettle, in agitated kettle into
Row stirs for the first time, dry mixing 20min, to ensure that each component is uniformly mixed, then carries out stirring for second, wet-mixing 45min, wet-mixing
Add water into raw material in the process, ensures that feed moisture content is 66.9%, obtain mixture；Mixture is sent by conveyer belt to bottling
Mixture is packed into flammulina velatipes culture bottle to shoulder by machine, and mixture bottling amount is 1010g, will be in culture bottle by puncher
Mixture is made a call to five holes and is covered after flattening, it is desirable that the smooth nothing of charge level collapses, five hole of culture bottle bottom can light transmission, when convenient for being inoculated with
Strain can reach culture bottle bottom；It is put into autoclave to sterilize, be sterilized by the way of high temperature and pressure, in entire sterilization process
Sterilization pressure >=0.15MPa, sterilizing program is 105 DEG C and keeps the temperature 60 minutes, and 122 DEG C sterilize 60 minutes, keep the temperature 10 minutes, complete to go out
Bacterium, automatic exhaust 1 hour are cooling；
(2) it is inoculated with
The culture bottle equipped with mixture is inoculated with after sterilizing, it is desirable that and temperature is 17 DEG C, inoculum concentration 40mL in bottle, via
Conveyer belt enters culture bottle culturing room；
(3) culturing room cultivates
Culturing room requires indoor clean, heat preservation, well-ventilated, need not illuminate, it is desirable that the shoulder temperature of flammulina velatipes culture bottle
Degree is 18 DEG C；It was cultivated to the 6th day just after inoculation, culture humidity is 70%, CO2Concentration is less than 2000ppm；After inoculation
It is cultivated in rear training within 7th day to 23 days, culture humidity is 78%, CO2Concentration should be controlled in 3000ppm, and through 22 days, mycelia was long
Full culture bottle；
(4) mycelium stimulation
Mycelia is subjected to mechanical mycelium stimulation；
(5) fertility room growth
Culture bottle after mycelium stimulation enters fertility room, and mycelia restores kink, after forming former base, sleeve when growing to 17 days, and set
Cylinder piece height 15.5cm, using growth in 9 days, mushroom body was grown to a little higher than sleeve piece, is harvested.Single bottle yield be 504g ±
2g。
It these are only specific embodiments of the present invention, be not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.Any modification made by all within the spirits and principles of the present invention,
Equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (5)
1. a kind of method for cultivating needle mushroom using sorghum flour mixture, it is characterized in that：
It is as follows：
(1) Enoki mushroom cultivation material dispensing
Percentage composition by weight, by raw material corncob 37%, cotton seed hulls 5%, wheat bran 5%, brewex's grains 5%, soybean skin 5%, sweet tea
Dish slag 4%, rice bran 26.5%, maize flour 5%, sorghum flour 5%, shellfish fossil 1.5% and calcium carbonate 1%, are added in agitated kettle,
First time stirring is carried out in agitated kettle, then dry mixing 20min carries out second and stirs, wet-mixing 45min, during wet-mixing to
In raw material plus water, guarantee feed moisture content are 66.9%, obtain mixture；Mixture is sent by conveyer belt to bottling machine, will be mixed
It closes material to be packed into flammulina velatipes culture bottle to shoulder, makes a call to five holes after being flattened the mixture in culture bottle by puncher and cover, put
Enter autoclave to sterilize, be sterilized by the way of high temperature and pressure, sterilization pressure >=0.15MPa in entire sterilization process, sterilizing
Program is 105 DEG C and keeps the temperature 60 minutes, and 122 DEG C sterilize 60 minutes, keep the temperature 10 minutes, completes sterilizing, and automatic exhaust 1 hour is cooling；
(2) it is inoculated with
The culture bottle equipped with mixture is inoculated with after sterilizing, it is desirable that temperature is 16 DEG C -18 DEG C in bottle, inoculum concentration 38mL-
42mL enters via conveyer belt by culture bottle culturing room；
(3) culturing room cultivates
Culturing room requires indoor clean, heat preservation, well-ventilated, need not illuminate, it is desirable that the shoulder temperature of flammulina velatipes culture bottle is
17℃-19.5℃；It was cultivated to the 6th day just after inoculation, culture humidity is 70%, CO2Concentration is less than 2000ppm；It connects
It is cultivated in rear training within the 7th day to 23 days after kind, culture humidity is 75%-80%, CO2Concentration should be controlled in 2000ppm-
3500ppm, through -23 days 22 days, mycelia covered with culture bottle；
(4) mycelium stimulation
Mycelia is subjected to mechanical mycelium stimulation；
(5) fertility room growth
Culture bottle after mycelium stimulation enters fertility room, and mycelia restores kink, after forming former base, sleeve when growing to 17 days, using 8
Its -10 days growth is harvested when mushroom body is grown to a little higher than sleeve piece.
2. the method according to claim 1 for cultivating needle mushroom using sorghum flour mixture, it is characterized in that：The needle mushroom
The specification of culture bottle is
3. the method according to claim 1 for cultivating needle mushroom using sorghum flour mixture, it is characterized in that：Mixture is bottled
Amount is 1000g-1020g.
4. the method according to claim 1 for cultivating needle mushroom using sorghum flour mixture, it is characterized in that：When punching,
Ask the smooth nothing of charge level to collapse, five hole of culture bottle bottom can light transmission, convenient for inoculation when strain can reach culture bottle bottom.
5. the method according to claim 1 for cultivating needle mushroom using sorghum flour mixture, it is characterized in that：Sleeve piece height
15.5cm, mushroom body is higher by sleeve piece 1cm when harvesting.
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Cited By (5)
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| CN109076879A (en) * | 2018-11-03 | 2018-12-25 | 上海品柔文化发展有限公司 | A kind of selenium-rich gold needle mushroom and its production method |
| CN110192496A (en) * | 2019-07-17 | 2019-09-03 | 江苏友康生态科技有限公司 | A kind of Grifola frondosa culture material formula |
| CN110663451A (en) * | 2019-09-16 | 2020-01-10 | 江苏华绿生物科技股份有限公司 | Application of novel palm fiber culture medium in industrial cultivation of white needle mushrooms |
| CN111296174A (en) * | 2020-03-28 | 2020-06-19 | 江苏华绿生物科技股份有限公司 | High-pressure sterilization method for needle mushroom cultivation bottles |
| CN113575277A (en) * | 2021-08-11 | 2021-11-02 | 江苏华绿生物科技股份有限公司 | Sorghum bran culture medium and application thereof in industrial cultivation of white flammulina velutipes |
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| CN110663451A (en) * | 2019-09-16 | 2020-01-10 | 江苏华绿生物科技股份有限公司 | Application of novel palm fiber culture medium in industrial cultivation of white needle mushrooms |
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| CN113575277A (en) * | 2021-08-11 | 2021-11-02 | 江苏华绿生物科技股份有限公司 | Sorghum bran culture medium and application thereof in industrial cultivation of white flammulina velutipes |
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|---|---|
| CN108293592B (en) | 2022-09-23 |
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