CN111296174A - High-pressure sterilization method for needle mushroom cultivation bottles - Google Patents
High-pressure sterilization method for needle mushroom cultivation bottles Download PDFInfo
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- CN111296174A CN111296174A CN202010232746.0A CN202010232746A CN111296174A CN 111296174 A CN111296174 A CN 111296174A CN 202010232746 A CN202010232746 A CN 202010232746A CN 111296174 A CN111296174 A CN 111296174A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/30—Accessories for use before inoculation of spawn, e.g. sterilisers
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Abstract
The invention discloses a method for sterilizing needle mushroom cultivation bottles under high pressure, which comprises the following steps: step one, starting an automatic sterilization program, vacuumizing for three times, and confirming the temperature rise condition in a sterilization pot after the completion; step two, confirming the temperature in the sterilization pot of 105-; and step three, automatically operating a sterilization program, raising the temperature to 123 ℃, requiring the pressure to be 0.120-0.140 MPa, sterilizing, keeping the temperature for 90-100 minutes, and stewing for 20 minutes. Can achieve effective sterilization time and improve the stability of industrial cultivation.
Description
Technical Field
The invention relates to a high-pressure sterilization method for needle mushroom cultivation bottles, and particularly relates to the technical field of industrial cultivation of edible mushrooms.
Background
The flammulina velutipes is the edible fungus variety with the highest industrial degree, and the industrial cultivation of the flammulina velutipes has strong competition due to early start, advanced technology and mature management. In recent years, the capacity of industrial needle mushroom cultivation bottles has been expanding to 1100cc, 1200cc, and the capacity has been increased to 1500 cc. The small-capacity needle mushroom cultivation bottles are easy to sterilize thoroughly under the high-pressure sterilization program, but the large-capacity needle mushroom cultivation bottles are easy to have the problem of insufficient effective sterilization time. Therefore, the improvement of the sterilization procedure to achieve the effective sterilization time is an important link in the production of the flammulina velutipes.
Disclosure of Invention
The invention aims to provide a method for autoclaving a needle mushroom cultivation bottle.
In order to achieve the purpose, the invention provides the following technical scheme: an autoclave sterilization method for needle mushroom cultivation bottles comprises the following steps: step one, starting an automatic sterilization program, vacuumizing for three times, and confirming the temperature rise condition in a sterilization pot after the completion;
step two, confirming the temperature in the sterilization pot of 105-;
and step three, automatically operating a sterilization program, raising the temperature to 123 ℃, requiring the pressure to be 0.120-0.140 MPa, sterilizing, keeping the temperature for 90-100 minutes, and stewing for 20 minutes.
Preferably, in the first step, after the vacuum pumping, the temperature of the inner pressure of the sterilization pot is increased to-0.055 MPa, and the vacuum pumping is started when the temperature is increased to-0.025 MPa.
Compared with the prior art, the invention has the following beneficial effects: under the condition of autoclaving, the effective sterilization time of the needle mushroom cultivation bottle is required to reach 120 ℃, and the material temperature is maintained for more than 60 minutes, so that the sterilization effect can be achieved. Therefore, the sterilization temperature is raised to 123 ℃ from the original 121 ℃, and the heat preservation time is raised to 90-100 minutes from 70 minutes, thereby achieving effective sterilization. Can achieve effective sterilization time and improve the stability of industrial cultivation.
Drawings
FIG. 1 is a graph showing the temperature change of a specific material in example 1 of the present invention;
FIG. 2 is a graph showing the temperature change of a concrete material in a comparative example of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely in the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
An autoclave sterilization method for needle mushroom cultivation bottles comprises the following steps: step one, randomly selecting one of 1500cc cultivation bottles entering a sterilization pot, putting a temperature recorder in the bottle, and tracking the temperature change condition of the culture material;
step two, starting an automatic sterilization program, vacuumizing for three times, starting heating up when the pressure in the sterilization pot is-0.055 MPa after vacuumizing, starting vacuumizing when the pressure is raised to-0.025 MPa, and confirming the temperature rise condition in the sterilization pot after the heating up is finished;
step three, confirming the temperature in the sterilization pot to be 105 ℃ and keeping the temperature for 30 minutes, and keeping the temperature at 116 ℃ and keeping the temperature for 45 minutes;
and step four, automatically operating a sterilization program, raising the temperature to 123 ℃, requiring the pressure to be 0.120MPa, sterilizing, keeping the temperature for 90 minutes, and stewing for 20 minutes.
And fifthly, discharging, taking out the temperature recorder, and analyzing the temperature change condition of the material.
The specific material temperature change is shown in figure 1, and the analysis of figure 1 shows that the effective sterilization time for keeping the temperature in the culture medium above 120 ℃ is 71 minutes, so that the effective sterilization can be achieved.
Example 2
An autoclave sterilization method for needle mushroom cultivation bottles comprises the following steps: step one, randomly selecting one of 1500cc cultivation bottles entering a sterilization pot, putting a temperature recorder in the bottle, and tracking the temperature change condition of the culture material;
step two, starting an automatic sterilization program, vacuumizing for three times, starting heating up when the pressure in the sterilization pot is-0.055 MPa after vacuumizing, starting vacuumizing when the pressure is raised to-0.025 MPa, and confirming the temperature rise condition in the sterilization pot after the heating up is finished;
step three, confirming the temperature in the sterilizer to be 107 ℃ and keeping the temperature for 35 minutes, and keeping the temperature at 117 ℃ for 50 minutes;
and step four, automatically operating a sterilization program, raising the temperature to 123 ℃, requiring the pressure to be 0.140MPa, sterilizing, keeping the temperature for 100 minutes, and stewing for 20 minutes.
And fifthly, discharging, taking out the temperature recorder, and analyzing the temperature change condition of the material.
Comparative example
A1500 CC needle mushroom cultivation bottle sterilization program comprises the following steps:
step one, starting an automatic sterilization program, vacuumizing for three times, controlling the pressure in the sterilization pot to be-0.055 MPa after vacuumizing, and confirming the temperature rise condition in the sterilization pot after the vacuumizing is finished;
step two, confirming the temperature in the sterilization pot to be 100 ℃ and keeping the temperature for 60 minutes;
step three, automatically operating a sterilization program, raising the temperature to 121 ℃, requiring the pressure to be 0.120-0.140 MPa, preserving the heat for 70 minutes, and stewing for 20 minutes; the specific material temperature change is shown in figure 2, and the analysis of figure 2 shows that the effective sterilization time for maintaining the temperature of above 120 ℃ in the culture medium is 53 minutes, and the effective sterilization time is insufficient.
Therefore, the sterilization method can achieve effective sterilization time and improve the stability of industrial cultivation.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (2)
1. An autoclave sterilization method for needle mushroom cultivation bottles is characterized in that: the specific autoclaving method comprises the following steps: step one, starting an automatic sterilization program, vacuumizing for three times, and confirming the temperature rise condition in a sterilization pot after the completion;
step two, confirming the temperature in the sterilization pot of 105-;
and step three, automatically operating a sterilization program, raising the temperature to 123 ℃, requiring the pressure to be 0.120-0.140 MPa, sterilizing, keeping the temperature for 90-100 minutes, and stewing for 20 minutes.
2. The autoclaving method of the needle mushroom cultivation bottle according to claim 1, wherein: in the first step, after vacuum pumping, the temperature of the sterilization pot is raised to-0.055 MPa, and the vacuum pumping is started when the temperature is raised to-0.025 MPa.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010232746.0A CN111296174A (en) | 2020-03-28 | 2020-03-28 | High-pressure sterilization method for needle mushroom cultivation bottles |
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| CN202010232746.0A CN111296174A (en) | 2020-03-28 | 2020-03-28 | High-pressure sterilization method for needle mushroom cultivation bottles |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112136604A (en) * | 2020-07-02 | 2020-12-29 | 江苏华骏生物科技有限公司 | Sterilization process for industrial cultivation of grifola frondosa |
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| CN1593086A (en) * | 2003-09-10 | 2005-03-16 | 上海浦东天厨菇业有限公司 | High temperature high pressure sterilization process for edible mushroom cultivation medium |
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| CN103004473A (en) * | 2012-12-29 | 2013-04-03 | 沈阳恒生农业发展有限公司 | Cooling technique for increasing inoculating yield in industrial production of bottled golden needle mushrooms |
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| CN103053325A (en) * | 2012-12-06 | 2013-04-24 | 青岛丰科生物科技有限公司 | Edible fungus industrialized cultivation method and sterilization process thereof |
| CN108293592A (en) * | 2017-10-17 | 2018-07-20 | 辽宁天赢生物科技股份有限公司 | A method of cultivating needle mushroom using sorghum flour mixture |
| CN110679395A (en) * | 2019-10-22 | 2020-01-14 | 江苏华绿生物科技股份有限公司 | Anti-pollution process in sterilization link |
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2020
- 2020-03-28 CN CN202010232746.0A patent/CN111296174A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1593086A (en) * | 2003-09-10 | 2005-03-16 | 上海浦东天厨菇业有限公司 | High temperature high pressure sterilization process for edible mushroom cultivation medium |
| CN101107911A (en) * | 2007-05-24 | 2008-01-23 | 镇江市丹徒区正东生态农业发展中心 | Culture material disinfecting method for factory culture of edible fungus |
| CN101502217A (en) * | 2009-03-13 | 2009-08-12 | 镇江市丹徒区正东生态农业发展中心 | Process sterilization method for culture medium of edible fungus |
| CN101904272A (en) * | 2010-08-17 | 2010-12-08 | 超大(上海)食用菌有限公司 | Sterilization method for producing culture material for hypsizigus marmoreus in factory |
| CN103053325A (en) * | 2012-12-06 | 2013-04-24 | 青岛丰科生物科技有限公司 | Edible fungus industrialized cultivation method and sterilization process thereof |
| CN103004473A (en) * | 2012-12-29 | 2013-04-03 | 沈阳恒生农业发展有限公司 | Cooling technique for increasing inoculating yield in industrial production of bottled golden needle mushrooms |
| CN103004472A (en) * | 2012-12-29 | 2013-04-03 | 沈阳恒生农业发展有限公司 | Sterilization process method for bottle-planted needle mushroom factory production |
| CN108293592A (en) * | 2017-10-17 | 2018-07-20 | 辽宁天赢生物科技股份有限公司 | A method of cultivating needle mushroom using sorghum flour mixture |
| CN110679395A (en) * | 2019-10-22 | 2020-01-14 | 江苏华绿生物科技股份有限公司 | Anti-pollution process in sterilization link |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112136604A (en) * | 2020-07-02 | 2020-12-29 | 江苏华骏生物科技有限公司 | Sterilization process for industrial cultivation of grifola frondosa |
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