CN111557210A

CN111557210A - Flammulina velutipes root culture medium used for cultivation of flammulina velutipes

Info

Publication number: CN111557210A
Application number: CN202010471944.2A
Authority: CN
Inventors: 冯占; 丁惠; 李秋月; 李贺文; 余养朝
Original assignee: Jiangsu Chinagreen Biological Technology Co ltd
Current assignee: Jiangsu Chinagreen Biological Technology Co ltd
Priority date: 2020-05-29
Filing date: 2020-05-29
Publication date: 2020-08-21

Abstract

The invention discloses a cultivation process of flammulina velutipes by using a flammulina velutipes root culture medium, which comprises the following raw material components: the invention relates to a needle mushroom, which comprises needle mushroom root, corncob, rice bran, dried bean dregs, cottonseed hull, brewer's grains, soybean hull, shell powder, light calcium carbonate and beet pulp, and has the beneficial effects that: the method can reduce the use amount of other raw materials, reduce the cost, reduce the waste generated in the processing process of the flammulina velutipes, realize the recyclable production of factories and promote the healthy and rapid development of the edible fungus industry.

Description

Flammulina velutipes root culture medium used for cultivation of flammulina velutipes

Technical Field

The invention relates to the technical field related to industrial cultivation of edible fungi, in particular to a cultivation process of a needle mushroom root culture medium for needle mushrooms.

Background

The flammulina velutipes is the edible fungus variety with the highest industrial degree, and the industrial cultivation of the flammulina velutipes has strong competition due to early start, advanced technology and mature management. In recent years, the prices of raw materials such as rice bran, wheat bran, and corncobs, which are main raw materials for edible fungus cultivation, have been increasing, and cottonseed hulls, which are widely used in edible fungus cultivation, run the risk of enriching pesticides and heavy metals. Under the background, the preparation of the low-cost culture medium meeting the food safety requirement, and the improvement of the product quality, the improvement of the production efficiency and the reduction of the production cost are the primary tasks to be solved in the industrial production of the edible fungi.

Disclosure of Invention

The invention aims to solve the existing problems and provides a cultivation process of a flammulina velutipes root culture medium for flammulina velutipes, which takes the waste flammulina velutipes roots as raw materials, adopts drying and physical crushing methods to ensure that the waste flammulina velutipes roots meet the requirements of the flammulina velutipes culture medium on water content and granularity for reutilization, can reduce the usage amount of other raw materials, reduce the cost, reduce the waste generated in the flammulina velutipes processing process, realize the recyclable production of factories and promote the healthy and rapid development of the edible fungi industry.

The technical scheme adopted by the invention for realizing the technical purpose is as follows: a needle mushroom root culture medium is used for a needle mushroom cultivation process, and is prepared from needle mushroom roots, corncobs, rice bran, dried bean dregs, cottonseed hulls, brewer's grains, soybean hulls, shell powder, light calcium carbonate and beet pulp, wherein the raw materials comprise the following components in parts by weight: 3-5 parts of needle mushroom roots, 30-40 parts of corncobs, 20-30 parts of rice bran, 10-20 parts of bran, 3-5 parts of dry bean dregs, 6-8 parts of cottonseed hulls, 3-4 parts of brewer's grains, 3-5 parts of soybean hulls, 1-3 parts of shell powder, 1-2 parts of light calcium carbonate and 3-6 parts of beet pulp, and the preparation process comprises six process steps: step 1: sterilization treatment, step 2: culturing in a culture room, and step 3: scratching and promoting buds, and step 4: light suppression and wind suppression processing, step 5: promoting elongation of stipe, and step 6: and (6) harvesting.

Further, the specific process steps of the sterilization treatment in the step 1 are as follows: and (3) filling the novel needle mushroom roots into a cultivation bottle, performing high-pressure steam sterilization treatment on the needle mushroom roots, and cooling to room temperature for later use after the sterilization treatment is finished.

Further, the specific process steps of the culture chamber culture in the step 2 are as follows: inoculating liquid strains into the culture bottles, culturing in a culture room at the culture temperature of 13-15 ℃ and the air humidity of 60-90% for 20-23 days, and inoculating the liquid strains in the step 2 in an amount of 30-35 mL for each culture bottle.

Further, the mushroom scratching and bud forcing process in the step 3 comprises the following specific steps: after the hyphae cultured in the step 2 overgrows the cultivation bottle, performing mycelium stimulation treatment, removing old hyphae on the material surface, forming mechanical stimulation, then moving the cultivation bottle to a growing room for bud promotion, and after 4-6 days, covering mushroom buds on the material surface, wherein the bud promotion conditions are as follows: the room temperature is 15-16 ℃, the air humidity is 85-95%, and the concentration of CO2 is 3000-4000 ppm.

Further, the specific process steps of the light inhibition and wind inhibition treatment in the step 4 are as follows: after the bud forcing is finished, performing photoinhibition and photoinhibition on mushroom buds, controlling the concentration of CO2 to be 3000-20000 ppm, performing gradient cooling to be 4-5 ℃, and enabling the mushroom buds to grow into mushroom buds, wherein the gradient cooling is performed at a cooling rate of 2-4 ℃ every day, the photoinhibition illumination intensity is 100Lux, the photoinhibition blowing rate is 1-3 m/s, and the air volume is 150m 3/h.

Further, the specific process steps for promoting the elongation of the stipe in the step 5 are as follows: and (4) when the mushroom buds grow out of the bottle mouth of 1-2 cm in the step 4, increasing the concentration of CO2 to be more than 10000ppm, and promoting the elongation of the mushroom stalks of the needle mushrooms.

Further, the specific process steps of harvesting in the step 6 are as follows: when the needle mushrooms grow to 15-16 cm, the needle mushrooms reach the harvesting height, and harvesting is carried out.

Experiments show that compared with the prior art, the invention has the following advantages:

(1) the needle mushroom roots are used as byproducts of needle mushroom industrial cultivation, and are applied to a culture medium through drying and physical crushing methods, so that the treatment of wastes by a factory is reduced, the recyclable production of the factory is realized, and the environment is protected and the cost is saved.

(2) The needle mushroom roots are used as drying products, which are not only beneficial to storage, but also can be automatically crushed according to the required granularity during production and use, and are convenient to use.

(3) The protein content of needle mushroom root is close to that of rice bran protein required by production, and the needle mushroom root protein can provide nutrition for needle mushroom production.

(4) The yield per unit and the quality of the produced needle mushroom by taking the needle mushroom roots as the raw materials to reduce the use amount of other raw materials are not obviously different from those of the needle mushroom produced by a culture medium without the needle mushroom roots.

Detailed Description

As further illustrated below, the starting materials used in the following steps are all conventional in the art or commercially available.

S1, filling novel needle mushroom roots into a cultivation bottle, performing high-pressure steam sterilization treatment on the needle mushroom roots, and cooling to room temperature for later use after the sterilization treatment is finished;

s2, inoculating liquid strains into culture bottles, culturing in a culture room at the culture temperature of 13-15 ℃ and the air humidity of 60-90% for 20-23 days, wherein the inoculation amount of the liquid strains in the step 2 is 30-35 mL for each culture bottle;

s3, after the hyphae cultured in the step 2 overgrow a culture bottle, performing mycelium stimulation treatment, removing old hyphae on the material surface, forming mechanical stimulation, then moving the culture bottle to a growing room for bud promotion, and after 4-6 days, covering mushroom buds on the material surface, wherein the bud promotion conditions are as follows: the room temperature is 15-16 ℃, the air humidity is 85-95%, and the concentration of CO2 is 3000-4000 ppm.

And S4, after bud forcing is finished, carrying out light inhibition and wind inhibition on mushroom buds, controlling the concentration of CO2 to be 3000-20000 ppm, carrying out gradient cooling to be 4-5 ℃, and enabling the mushroom buds to grow into mushroom buds, wherein the gradient cooling is carried out at a cooling rate of 2-4 ℃ every day, the light intensity of the light inhibition is 100Lux, the blowing rate of the wind inhibition is 1-3 m/s, and the air volume is 150m 3/h.

S5, when mushroom buds grow out of the bottle mouth in the step 4 and are 1-2 cm in length, the concentration of CO2 is increased to be more than 10000ppm, and the elongation of needle mushroom stipes is promoted.

S6, when the needle mushrooms grow to 15-16 cm, the needle mushrooms reach the harvesting height, and harvesting is conducted.

Various other changes and modifications to the above-described embodiments and concepts may occur to those skilled in the art, and all such changes and modifications are intended to be included within the scope of the present invention as defined in the appended claims.

Claims

1. A cultivation process of flammulina velutipes by using a flammulina velutipes root culture medium is characterized by comprising the following steps: the preparation raw materials comprise needle mushroom roots, corncobs, rice bran, dried bean dregs, cottonseed hulls, brewer's grains, soybean hulls, shell powder, light calcium carbonate and beet pulp, and the raw materials comprise the following components in parts by weight: 3-5 parts of needle mushroom roots, 30-40 parts of corncobs, 20-30 parts of rice bran, 10-20 parts of bran, 3-5 parts of dry bean dregs, 6-8 parts of cottonseed hulls, 3-4 parts of brewer's grains, 3-5 parts of soybean hulls, 1-3 parts of shell powder, 1-2 parts of light calcium carbonate and 3-6 parts of beet pulp, and the preparation process comprises six process steps:

step 1: sterilizing treatment

Step 2: culturing in a culture room

And step 3: scratching of fungi and promotion of budding

And 4, step 4: light and wind suppression treatment

And 5: promotion of stipe elongation

Step 6: and (6) harvesting.

2. The needle mushroom root culture medium used for the needle mushroom cultivation process according to claim 1, characterized in that: the specific process steps of the sterilization treatment in the step 1 are as follows: and (3) filling the novel needle mushroom roots into a cultivation bottle, performing high-pressure steam sterilization treatment on the needle mushroom roots, and cooling to room temperature for later use after the sterilization treatment is finished.

3. The needle mushroom root culture medium used for the needle mushroom cultivation process according to claim 1, characterized in that: the specific process steps of the culture room culture in the step 2 are as follows: inoculating liquid strains into the culture bottles, culturing in a culture room at the culture temperature of 13-15 ℃ and the air humidity of 60-90% for 20-23 days, and inoculating the liquid strains in the step 2 in an amount of 30-35 mL for each culture bottle.

4. The needle mushroom root culture medium used for the needle mushroom cultivation process according to claim 1, characterized in that: the specific process steps of scratching and promoting buds in the step 3 are as follows: after the hyphae cultured in the step 2 overgrows the cultivation bottle, performing mycelium stimulation treatment, removing old hyphae on the material surface, forming mechanical stimulation, then moving the cultivation bottle to a growing room for bud promotion, and after 4-6 days, covering mushroom buds on the material surface, wherein the bud promotion conditions are as follows: the room temperature is 15-16 ℃, the air humidity is 85-95%, and the concentration of CO2 is 3000-4000 ppm.

5. The needle mushroom root culture medium used for the needle mushroom cultivation process according to claim 1, characterized in that: the specific process steps of the light inhibition and wind inhibition treatment in the step 4 are as follows: after the bud forcing is finished, performing photoinhibition and photoinhibition on mushroom buds, controlling the concentration of CO2 to be 3000-20000 ppm, performing gradient cooling to be 4-5 ℃, and enabling the mushroom buds to grow into mushroom buds, wherein the gradient cooling is performed at a cooling rate of 2-4 ℃ every day, the photoinhibition illumination intensity is 100Lux, the photoinhibition blowing rate is 1-3 m/s, and the air volume is 150m 3/h.

6. The needle mushroom root culture medium used for the needle mushroom cultivation process according to claim 1, characterized in that: the specific process steps for promoting the elongation of the stipe in the step 5 are as follows: and (4) when the mushroom buds grow out of the bottle mouth of 1-2 cm in the step 4, increasing the concentration of CO2 to be more than 10000ppm, and promoting the elongation of the mushroom stalks of the needle mushrooms.

7. The needle mushroom root culture medium used for the needle mushroom cultivation process according to claim 1, characterized in that: the specific process steps of harvesting in the step 6 are as follows: when the needle mushrooms grow to 15-16 cm, the needle mushrooms reach the harvesting height, and harvesting is carried out.