CN104531803A - Production method of fine flour with high active hericium erinaceus polysaccharide content and comprehensive utilization method of byproduct of fine flour - Google Patents
Production method of fine flour with high active hericium erinaceus polysaccharide content and comprehensive utilization method of byproduct of fine flour Download PDFInfo
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- CN104531803A CN104531803A CN201410812492.4A CN201410812492A CN104531803A CN 104531803 A CN104531803 A CN 104531803A CN 201410812492 A CN201410812492 A CN 201410812492A CN 104531803 A CN104531803 A CN 104531803A
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Abstract
The invention discloses a production method of fine flour with high active hericium erinaceus polysaccharide content and a comprehensive utilization method of a byproduct of the fine flour, belonging to the technical field of bioengineering. The production method comprises the following steps of inoculating a hericium erinaceus strain onto a corn and corncob solid culture medium to prepare hericium erinaceus mycelia, dissolving and extracting the mycelia to obtain a solution containing active hericium erinaceus polysaccharide, carrying out vacuum concentration on the solution containing the active hericium erinaceus polysaccharide, then, carrying out alcohol precipitation to obtain an active hericium erinaceus polysaccharide sediment, and processing to prepare fine flour containing 85% of the active hericium erinaceus polysaccharide; and recovering separated filter residues and separated filtrate sequentially generated in the process of producing an active hericium erinaceus polysaccharide product, and processing to obtain a byproduct, namely a hericium-erinaceus-containing high-quality feed additive for the breeding industry, a hericium erinaceus drink or a hericium erinaceus wine. The production method has the advantages of stable process, high yield, high hericium erinaceus polysaccharide content (up to 85%), high product quality and no any wastes in the whole process.
Description
Technical field
The method of comprehensive utilization of high-content hericium erinaceum polysaccharide fine powder production of the present invention and byproduct thereof, belongs to technical field of bioengineering.
Background technology
Since ancient times, Hericium erinaceus (Bull. Ex Fr.) Pers. is as the jewellery of Traditional Chinese Medicine, be endowed inscrutable mystery flavour, along with the researchdevelopment of modern medicine, people have fully realized nucleus---the polysaccharide of Hericium erinaceus (Bull. Ex Fr.) Pers. as medicine, function of health food, and Hericium erinaceus (Bull. Ex Fr.) Pers. can be adopted to obtain them for starting material with modern biotechnology, the processing method obtaining polysaccharide in recent years emerges in an endless stream, but substantially can be divided into following two classes:
1, from hericium erinaceus fruiting body, Hericium erinaceus (Bull. Ex Fr.) Pers. fungi activity polysaccharide is produced by water extraction process.
Ordinary method is that hericium erinaceus fruiting body raw material crushing is become block, and add water, decoct under normal pressure and extract filtrate, its residue carries out second time again and decocts extraction, forms product by concentrated for filtrate.
At the beginning of the eighties, Taiwan Li Jin wood, Wang Zhijie separation and Extraction from hericium erinaceus fruiting body water-soluble Hericium erinaceus (Bull. Ex Fr.) Pers. fungi activity polysaccharide, in the product of extraction, polysaccharide content is 40%.
2, Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide is produced with biotechnology fermentation technique.
Prior art adopts liquid fermentation technology to produce Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide, adopt the substratum aqueous solution of conventional carbonaceous sources, nitrogenous source, inorganic salt and VITAMIN, hedgehog fungus bacterial is inoculated in liquid nutrient medium, again under certain condition, after liquid fermenting, from fermented liquid and mycelium, obtain Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide.
Summary of the invention
The object of this invention is to provide the method for comprehensive utilization of the production of a kind of high-content hericium erinaceum polysaccharide fine powder and byproduct thereof, to obtain high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide and byproduct thereof, and production whole process is without waste output.
Technical solution of the present invention:
The method of comprehensive utilization of high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder production of the present invention and byproduct thereof, the corn being rich in rare elements germanium and selenium and the corn cob solid shaped bodies substratum of selecting pollution-free mountain area soil and weather ambient growth, through inoculation hedgehog fungus bacterial, prepare hericium mycelium, by dissolving, lixiviate mycelium and extracting obtains containing Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide solution, this is analysed by alcohol containing after Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide solution for vacuum concentration, the Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide throw out obtained, the obtained polysaccharide content of processing up to 85% Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder; And reclaim the separation filter residue and separating filtrate that successively produce in preparation Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide product process, reclaim the byproduct be processed as containing Hericium erinaceus (Bull. Ex Fr.) Pers., its processing step is as follows:
One, solid full particulate media is prepared: with corn and corn cob for raw material, permeablely be dipped to moisture 50-60%, steam removes the pretreated loose corn of mould and corn cob granule base-material, with the inorganic salt containing K+, Ca++, Zn++, Mg++ that routine uses, Homogeneous phase mixing makes slightly acidic solid shaped bodies substratum;
Two, hericium mycelium is cultivated: be placed in sealed glass container by above-mentioned substratum, after autoclaving process, in gnotobasis access hedgehog fungus bacterial, in sealing bacteriumization culture environment, the surface growth of solid shaped bodies particulate media is made to go out plentiful hericium mycelium, take out mycelium, fresh goods namely with or oven dry to make dry product for subsequent use;
Three, lixiviate and preparation Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide product
1) to specify ratio 100 DEG C of above-mentioned hericium myceliums of weakly alkaline deionized water dissolving, extracted many times Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide, each lixiviate 50-60 minute, filter residue after filtering separation lixiviate Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide, obtain containing Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide solution;
2) Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide filtrate is contained by above-mentioned, vacuum concentration, obtain Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide concentrated solution, in this concentrated solution, add 3-4 times of high purity grain wine to weight of material again carry out alcohol and analyse, filtrate analysed by filtering separation alcohol, the Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide throw out obtained, after drying, the obtained high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder of processing.
Described hericium mycelium sealing bacteriumization culture environment, its temperature about 25 DEG C, relative humidity 75-85%.
The corn of the full particulate media of described preparation solid and the raw material of corn cob come from without dirty ecotope, are naturally rich in rare elements germanium and selenium, and its corn and corn cob granule mass ratio are 1:1.
The number of times at least twice of described dissolving hericium mycelium lixiviate Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide.
The described vacuum concentration condition containing Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide filtrate is temperature 55 DEG C-70 DEG C, pressure 0.07-0.09Mpa.
The density that described vacuum concentration obtains Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide concentrated solution is 1.1-1.3.
Described lixiviate Hericium erinaceus (Bull. Ex Fr.) Pers. deionized water and mycelium fresh goods ratio are (W/W) 3-3.5:1.
Described lixiviate Hericium erinaceus (Bull. Ex Fr.) Pers. deionized water and mycelium dry product ratio are (W/W) 6-6.5:1.
Described lixiviate is separated filter residue and separating filtrate with successively generation in preparation Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide product process, reclaims and is processed as byproduct, as the high-quality feed additive of aquaculture, and Hericium erinaceus (Bull. Ex Fr.) Pers. or Hericium erinaceus (Bull. Ex Fr.) Pers. Wine drink.
The present invention has significant technological innovation:
1, comprehensive utilization process of the present invention, adopt solid shaped bodies particle to make substratum zymotechnique, process stabilizing, productive rate is high, and Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide content is high, and at least polysaccharide reaches 85%, and product quality is excellent, has significant economic benefit.
2, comprehensive utilization process of the present invention is the ecological process pattern of enclosed raw material-product-raw material-byproduct, except major product Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide, reclaim relevant output object in its technological process: reclaim filter residue and be processed into poultry and livestock feed additive simultaneously; The deionized water reclaiming concentration process evaporation is prepared high purity Hericium erinaceus (Bull. Ex Fr.) Pers. dew protective foods drink and makes face nourishing skin care products raw material; The grain wine liquid material after precipitation analysed by recovery alcohol, prepares the various Hericium erinaceus (Bull. Ex Fr.) Pers. Wine drink containing Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide effective ingredient.Whole technological process does not produce any waste, does not produce any pollution to soil, water body and air, has extremely significantly social benefit.
3, the culture medium raw material that adopts of comprehensive utilization process of the present invention and bacterial classification all come from natural green crop without the growth of dye ecotope and bacterial strain, and whole production process does not add any chemical pigment and additive, ensure that the natural, green of product and high-quality.
Embodiment
Now further illustrate the present invention how to implement in conjunction with the embodiments:
Embodiment 1
Select west place in Hubei to bestow favour corn, impurity elimination, be dipped to corn moisture 40%, for subsequent use after steam sterilizing process.Get above-mentioned corn and corn cob granule 2500 grams, add 30 grams, edible plaster powder, add A, R level magnesium sulfate, zinc sulfate and each 10 grams of potassium primary phosphate, abundant uniform mixing, PH is adjusted to be 6.5, being respectively charged into 5 volumes is in the vial of 500ml, sealing is placed in pressure kettle, 0.157MPa sterilized under pressure 1.5 hours, pressurize 3.5 hours, treat Pressure Drop to zero, temperature is down to 25 DEG C, in disinfecting action district, by the preprepared wild hedgehog fungus bacterial of edible fungi inoculation method access, temperature 25 DEG C is given in sealing, humidity 75-85%, without the cultivation indoor cultivation 30 days of direct sunlight, the mycelium taken out, dry in the air dry oven of 60 DEG C ± 1 DEG C, obtain mycelium dry product 1056 grams.
Extract in bucket at stainless steel and add deionized water 6 kilograms, PH is adjusted to be 7.8, be heated to 100 DEG C take 1 kilogram of hericium mycelium dry product and be placed in the stainless steel well-bucket of mesh, well-bucket is slowly immersed in above-mentioned deionized water, after lixiviate twice, mention well-bucket, filter, reclaim filter residue, the filtrate of merging being placed in concentration tank under 0.09MPa pressure, being concentrated into proportion is 1.2, solution to be concentrated is cooled to room temperature and proceeds in stainless steel cask again, 75 degree are added in bucket, pure grain wine 6 kilograms, limit edged stirs, Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide is fully precipitated, leave standstill, precipitation separation, it is dried in 60 DEG C of air dry ovens and weighs, obtain Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide 32.6 grams, pulverize precision work again and obtain Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder product.And obtain the grain wine liquid material about 8 kilograms of Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide effective ingredient.The output object that corresponding each processing step reclaims by the corn produced respectively containing Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide effective ingredient and corn cob fodder additives, high purity Hericium erinaceus (Bull. Ex Fr.) Pers. juice drink, and the Hericium erinaceus (Bull. Ex Fr.) Pers. Wine drink of preparation containing Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide effective ingredient.
Embodiment 2
Select corn and the corn cob decon of pollution-free ecological ambient growth, soak to moisture content 60%, for subsequent use with steam sterilizing 20 minutes.
Get the above-mentioned corn for preparing and corn cob 1000 grams, add usual amounts calcium carbonate, potassium primary phosphate, zinc sulfate, magnesium sulfate and micro-organic germanium and selenium, fully mix evenly, PH is adjusted to be 6.5, being respectively charged into 5 volumes is in 500ml vial, being tamping bottleneck to be placed in pressure kettle under 0.157 MPa, sterilizing 1.5 is little is ground, pressurize 3.5 hours, treat Pressure Drop to zero, temperature is down to 25 DEG C, take out, in disinfecting action district, by the preprepared wild Hericium erinaceus (Bull. Ex Fr.) Pers. fungi strain of edible fungi inoculation method access, temperature 25 DEG C is given in sealing, relative humidity 70%, the nothing that lucifuge is ventilated cultivates indoor cultivation 30 days, the mycelium taken out, this fresh goods mycelium is placed with in the stainless steel well-bucket of mesh, extract in bucket at stainless steel, add 3 kilograms of deionized waters, PH is adjusted to be 7.8, be heated to 100 DEG C, mycelial well-bucket will be housed be placed in one, lixiviate is filtered for 45 minutes, repeat lixiviate more once, filter, reclaim filter residue, by merge filtrate in concentration tank under 0.07MPa pressure, be concentrated into proportion 1.3, solution to be concentrated is cooled to 20 DEG C, proceed in stainless steel cask, add 3 kilograms, 75 DEG C of pure grain wine, limit edged stirs, abundant precipitation, precipitation separation, dry, obtain Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide 20 grams.Be processed into fine powder product, the filter residue of recovery is processed into fodder additives, the deionized water of the vacuum concentration evaporation of recovery, and preparation high purity hericium erinaceum drink, the grain wine liquid material after precipitation analysed by recovery alcohol prepares various Hericium erinaceus (Bull. Ex Fr.) Pers. wine.
Claims (9)
1. the method for comprehensive utilization of the production of high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder and byproduct thereof, it is characterized in that, select the corn being rich in rare elements germanium and selenium and the corn cob solid shaped bodies substratum of pollution-free mountain area soil and weather ambient growth, through inoculation hedgehog fungus bacterial, prepare hericium mycelium, by dissolving, lixiviate mycelium and extracting obtains containing Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide solution, this is analysed by alcohol containing after Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide solution for vacuum concentration, the Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide throw out obtained, the obtained polysaccharide content of processing up to 85% Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder, and reclaim the separation filter residue and separating filtrate that successively produce in preparation Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide product process, reclaim the byproduct be processed as containing Hericium erinaceus (Bull. Ex Fr.) Pers., its processing step is as follows:
One, solid full particulate media is prepared: with corn and corn cob for raw material, permeablely be dipped to moisture 50-60%, steam removes the pretreated loose corn of mould and corn cob granule base-material, with the inorganic salt containing K+, Ca++, Zn++, Mg++ that routine uses, Homogeneous phase mixing makes slightly acidic solid shaped bodies substratum;
Two, mycelium is cultivated: be placed in sealed glass container by above-mentioned substratum, after autoclaving process, in gnotobasis access hedgehog fungus bacterial, in sealing bacteriumization culture environment, the surface growth of solid shaped bodies particulate media is made to go out plentiful hericium mycelium, take out mycelium, fresh goods namely with or oven dry to make dry product for subsequent use;
Three, lixiviate and preparation Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide product
1) to specify ratio 100 DEG C of weakly alkaline deionized water dissolving mycelium, extracted many times Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide, each lixiviate 50-60 minute, filter residue after filtering separation lixiviate Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide, obtain containing Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide solution;
2) Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide filtrate is contained by above-mentioned, vacuum concentration, obtain Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide concentrated solution, in this concentrated solution, add 3-4 times of high purity grain wine to weight of material again carry out alcohol and analyse, filtrate analysed by filtering separation alcohol, the Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide throw out obtained, after drying, the obtained high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder of processing.
2. the method for comprehensive utilization of high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder production according to claim 1 and byproduct thereof, is characterized in that, hericium mycelium sealing bacteriumization culture environment, its temperature about 25 DEG C, relative humidity 75-85%.
3. the method for comprehensive utilization of high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder production according to claim 1 and byproduct thereof, it is characterized in that, the corn of the full particulate media of preparation solid and the raw material of corn cob come from without dirty ecotope, naturally be rich in rare elements germanium and selenium, its corn and corn cob granule mass ratio are 1:1.
4. the method for comprehensive utilization of high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder production according to claim 1 and byproduct thereof, is characterized in that, dissolves the number of times at least twice of hericium mycelium lixiviate Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide.
5. the method for comprehensive utilization of high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder production according to claim 1 and byproduct thereof, it is characterized in that, the vacuum concentration condition containing Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide filtrate is temperature 55 DEG C-70 DEG C, pressure 0.07-0.09Mpa.
6. the method for comprehensive utilization of high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder production according to claim 1 and byproduct thereof, it is characterized in that, the density that vacuum concentration obtains Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide concentrated solution is 1.1-1.3.
7. the method for comprehensive utilization of high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder production according to claim 1 and byproduct thereof, it is characterized in that, lixiviate Hericium erinaceus (Bull. Ex Fr.) Pers. deionized water and mycelium fresh goods ratio are (W/W) 3-3.5:1.
8. high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder according to claim 1 is produced and the method for comprehensive utilization of byproduct, and it is characterized in that, carrying Hericium erinaceus (Bull. Ex Fr.) Pers. deionized water with mycelium dry product ratio is (W/W) 6-6.5:1.
9. the method for comprehensive utilization of high-content Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide fine powder production according to claim 1 and byproduct thereof, it is characterized in that, lixiviate with successively produce in preparation Hericium erinaceus (Bull. Ex Fr.) Pers. active polysaccharide product process be separated filter residue and separating filtrate, reclaim the byproduct be processed as the high-quality feed additive of aquaculture and Hericium erinaceus (Bull. Ex Fr.) Pers., Hericium erinaceus (Bull. Ex Fr.) Pers. Wine drink.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103435713A (en) * | 2013-08-29 | 2013-12-11 | 傅玉 | Comprehensive utilization production process of high-content cordyceps polysaccharide fine powder and by-products thereof |
| CN108676647A (en) * | 2018-05-22 | 2018-10-19 | 福鼎市西坑孔家茶业有限公司 | A kind of peach blossom Spirit manufacture craft |
| CN112125979A (en) * | 2020-08-25 | 2020-12-25 | 福州康来生物科技有限公司 | Extraction method of hericium erinaceus polysaccharide |
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| CN1537947A (en) * | 2003-10-23 | 2004-10-20 | 武汉绿十字生物工程有限公司 | Comprehensive utilization production technology of high content of glossg ganoderma polysaccharide refined powder and its by product |
| US20060013825A1 (en) * | 2004-07-16 | 2006-01-19 | Medimush Aps | Immune modulating compounds from fungi |
| CN103435713A (en) * | 2013-08-29 | 2013-12-11 | 傅玉 | Comprehensive utilization production process of high-content cordyceps polysaccharide fine powder and by-products thereof |
| CN103725730A (en) * | 2013-12-27 | 2014-04-16 | 内蒙古科技大学 | Refining technology of mycelia polysaccharides of selenium-enriched hericium erinaceus |
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- 2014-12-24 CN CN201410812492.4A patent/CN104531803A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1537947A (en) * | 2003-10-23 | 2004-10-20 | 武汉绿十字生物工程有限公司 | Comprehensive utilization production technology of high content of glossg ganoderma polysaccharide refined powder and its by product |
| US20060013825A1 (en) * | 2004-07-16 | 2006-01-19 | Medimush Aps | Immune modulating compounds from fungi |
| CN103435713A (en) * | 2013-08-29 | 2013-12-11 | 傅玉 | Comprehensive utilization production process of high-content cordyceps polysaccharide fine powder and by-products thereof |
| CN103725730A (en) * | 2013-12-27 | 2014-04-16 | 内蒙古科技大学 | Refining technology of mycelia polysaccharides of selenium-enriched hericium erinaceus |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103435713A (en) * | 2013-08-29 | 2013-12-11 | 傅玉 | Comprehensive utilization production process of high-content cordyceps polysaccharide fine powder and by-products thereof |
| CN108676647A (en) * | 2018-05-22 | 2018-10-19 | 福鼎市西坑孔家茶业有限公司 | A kind of peach blossom Spirit manufacture craft |
| CN112125979A (en) * | 2020-08-25 | 2020-12-25 | 福州康来生物科技有限公司 | Extraction method of hericium erinaceus polysaccharide |
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