CN105110900A - Anti-insect pest foliar fertilizer prepared from fermentation liquor and preparation method for anti-insect pest foliar fertilizer - Google Patents

Anti-insect pest foliar fertilizer prepared from fermentation liquor and preparation method for anti-insect pest foliar fertilizer Download PDF

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Publication number
CN105110900A
CN105110900A CN201510470670.4A CN201510470670A CN105110900A CN 105110900 A CN105110900 A CN 105110900A CN 201510470670 A CN201510470670 A CN 201510470670A CN 105110900 A CN105110900 A CN 105110900A
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China
Prior art keywords
insect pest
anti
water
foliar fertilizer
fermented liquid
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CN201510470670.4A
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Chinese (zh)
Inventor
邢建生
王晓亭
吴德清
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马鞍山科邦生态肥有限公司
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Priority to CN201510470670.4A priority Critical patent/CN105110900A/en
Publication of CN105110900A publication Critical patent/CN105110900A/en

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Abstract

The invention discloses an anti-insect pest foliar fertilizer prepared from fermentation liquor and a preparation method for the anti-insect pest foliar fertilizer. The anti-insect pest foliar fertilizer is prepared from the following raw materials in parts by weight: rice bran, industrial corn flour, dry and yellow leaves, coal ash, soybean meal, an EM inoculant, saturated lime water, urea, monopotassium phosphate, melia azedarach extraction liquor, zinc sulfate, a precipitator and a proper amount of water. By adopting a liquid fermentation technology, fermented thalli are broken to release nutrients and some residual cytotoxin and antibacterial components by virtue of ultrasonic waves, so that plant diseases and insect pests in the extent can be effectively prevented at once and the occurrence rate of plant diseases and insect pests is reduced.

Description

Insect pest foliar nutrient prepared by a kind of fermented liquid and preparation method thereof

Technical field

The invention belongs to the Technology field of fertilizer, be specifically related to insect pest foliar nutrient prepared by a kind of fermented liquid and preparation method thereof.

Background technology

Liquid fermenting is one of modern biotechnology, it refers in biochemical reactor, natural imitation circle is by the oxygen of edible medicinal fungus in residue process required for necessary carbohydrate, organic and inorganic some trace elements of compound, inorganic salt etc. containing nitrogen and other thalline respiratory metabolism, and control suitable ambient conditions, carry out the prolific process of mycelia.The large-scale fermentation culture of industrialization is fermentative production, also known as deep drainpipe or reticent cultivation.Industrial fermentation is produced must adopt fermentor tank, and fermentation culture many employings triangular flask in laboratory.Contain thalline in the fermented liquid obtained, be decomposed and undecomposed nutritive ingredient, the meta-bolites that thalline produces.The direct hyoscine of fermented liquid or confession separation and Extraction, also can do liquid spawn.

The energy needed for the liquid of edible medicinal fungus can use cerelose, industrial starch and Rhizoma Dioscoreae powder etc.; Nitrogenous source can adopt soybean cake powder, dried silkworm chrysalis meal, bran powder etc.In order to reduce costs, usually also adopt part trade effluent to be surrogate, as soybean wastewater, raw material sources are extensive.The nutrition distribution pattern of liquid nutrient medium is even, is conducive to abundant contact and the absorption of mushroom nourishing body.Mycelia can grow, can discharge in time the metabolism waste gas of respiration generation inside and outside reactor under optimum temperuture, pH, oxygen and carbon-nitrogen ratio condition, therefore metabolic vigorous, mycelial growth division rapidly, the meta-bolites that a large amount of mycelium and polysaccharide, polypeptide etc. have physiologically active can be accumulated at short notice, cultivated by liquid state fermentation and obtain a large amount of mycelium and physiologically active substance and generally only need the time of 2 ~ 7 days and cell age is neat, and solid culture needs 30 ~ 50 days.Liquid strain can effectively reduce strain pollution rate, has flowing fast, easily disperses, a bacterium point is many, sprout the features such as fast, can effectively reduce the pollution of edible mushrooms in seeded process.

The product of microbial metabolism is broadly divided into two classes: primary metabolite and secondary metabolite, primary metabolite is that the breeding of microbe metabolism growth is necessary, can produce in whole life cycle more always, amino acid, Nucleotide, polysaccharide, lipid and VITAMIN etc. are produced in cell, and secondary metabolite microbiotic, alkaloid, bacteriotoxin and plant growth factor etc. that to be extracellular produce, of a great variety.

Summary of the invention

The present invention, in order to improve the quality of foliar nutrient, improves the utilization ratio of liquid fertilizer, and after inventing a kind of liquid state fermentation, the effective constituent extracted in fermented liquid makes a kind of high efficiency composition liquid fertilizer, and specific practice is as follows:

Insect pest foliar nutrient prepared by a kind of fermented liquid, it is characterized in that, be made up of the raw material of following weight part: rice bran 30 ~ 40, industrial corn powder 45 ~ 60, dry yellow leaf 10 ~ 15, flyash 5 ~ 8, dregs of beans 30 ~ 40, EM microbial inoculum 3 ~ 4, saturated limewater 10 ~ 15, urea 10 ~ 15, potassium primary phosphate 6 ~ 10, chinaberry extracting solution 3 ~ 7, zinc sulfate 2 ~ 4, precipitation agent 8 ~ 10 and appropriate water;

Described sinking agent is made up of the raw material of following weight part: lime powder 15 ~ 20,10% alum liquor 12 ~ 16,10% acetic acid solution 4 ~ 6, primary isoamyl alcohol 0.6 ~ 1.0, polyvinyl alcohol 6 ~ 8, tween 80 1.5 ~ 3, industrial Viscotrol C 2 ~ 4, ethanol 4 ~ 7 and water 50; The mixing of second alcohol and water is poured in reactor by preparation method, then polyvinyl alcohol is added, be heated to 70 ~ 80 DEG C of stirring and dissolving, then add 10% alum liquor, 10% acetic acid solution, primary isoamyl alcohol and lime powder, keep temperature of reaction kettle, adjustment rotating speed 150 ~ 200r/min stirs 20 ~ 35min, finally add tween 80 and industrial Viscotrol C simultaneously, insulated and stirred 30min is emulsion uniformly, obtains residue by funnel suction filtration, pulverizes to obtain pulvis sinking agent after oven dry.

A preparation method for insect pest foliar nutrient prepared by fermented liquid, is characterized in that, comprise following step:

(1) mixing rice bran, industrial corn powder, dry yellow leaf, flyash and dregs of beans, add the water of 10 ~ 12 times of its weight parts, ball milling 30 ~ 40min, to homogenate, pours in fermentor tank, carries out high-temperature sterilization process;

(2) EM microbial inoculum is added the water dilution of 5 times of its weight parts, be modulated into bacterium liquid, be then seeded in the fermentor tank of (1), adjustment pH5.5 ~ 7, temperature 25 ~ 28 DEG C, passes into sterile air simultaneously and stirs, and ferments 5 ~ 8 days;

(3) after (2) complete, fermented liquid is separately put in container, ultrasonic cell disruption instrument is utilized to carry out microbial disruption, with flowing water, container is lowered the temperature simultaneously, then precipitation agent mixing is added, then add saturated limewater, after stirring 8 ~ 10min, keep fermented liquid inertial motion, leave standstill 20 ~ 25min and carry out precipitation adsorption process voluntarily, after completing, supernatant liquor got by wooden dipper;

(4) mixing urea, potassium primary phosphate, chinaberry extracting solution, zinc sulfate and other remaining component, is added in the supernatant liquor described in (3), after stirring at room temperature is dissolved.

By alum, (main component is KAl (SO 4) 2gain enlightenment, its principle utilizes it in water, ionize out two metal ion species, and Al 3+be easy to hydrolysis, generate the aluminium hydroxide Al of colloid 3++ 3H 2o=Al (OH) 3+ 3H +(reversible), the adsorptive power of alumine hydroxide colloid is strong, the impurity that can suspend in planar water, and form precipitation, make clarification of water, although successful, but the throw out after sedimentation is easily loose, be again diffused in water, the present invention not only will make the fermented liquid after fermentation clarify, also must possess make not allow composition granule composition to condense speed is fast, settling velocity fast, easily extract the features such as supernatant liquor after sedimentation, the present invention proposes sinking agent for this reason.

The operation instruction of sinking agent: adopt 10% alum liquor to be the main component of precipitation agent, the sedimentation effect reached is best, then 10% acetic acid is adopted to have certain promoter action as the separating effect of sedimentation medium to inorganic sinking agent, but the reaction of acetic acid and fermented liquid is comparatively slow, period, some fermented liquid was overflowed with the bubble of densification, so add primary isoamyl alcohol when preparing sinking agent as defoamer, adding the minimum lime powder of particle diameter is stablizer, secondly the weight of sorbent material is increased, avoid the sinking agent settling velocity of one-component slow, adding polyvinyl alcohol makes each ingredient adsorption of Tai-Ace S 150 and sedimentation promoter action on the surface of lime powder particle as binding agent, after all the components has adsorbed, finally adding a certain amount of vegetables oil can effectively prevent sinking agent particle to be mutually sticked together, become agglomerate, influence effect, particle aggregation agent after completing just can with an overall form storage, transport and use, successful.

The present invention adopts liquid state fermentation to ferment, first initial centrifugation, the oarse-grained particulate matter of filtering, ultrasonic cell disintegration instrument is utilized by remaining liq to carry out fragmentation to the microorganism in liquid, simultaneously in order to prevent heat production in process, cause temperature to raise and adopt flowing water stream to lower the temperature, by special sinking agent, adsorption precipitation is carried out to the residue in fermentation and unfermentable solid waste, obtain comparatively pure liquid extracting solution, some mineral substance that add that finally can be composite carry out function improvement and nutritional supplementation, it is reduce molecule to the impact of blade that pure extracting solution is used as foliar nutrient, the easy blocking blades pore of finely ground particles, affect respiration, next is unfavorable for prevention and elimination of disease and pests.

Intracellular organic matter can be discharged: the organic compositions such as amino acid, Nucleotide, nucleic acid, carbohydrate, make the nutritive ingredient of extracting solution abundanter while the meaning of microbial disruption is microorganism cells cracking.

Beneficial effect of the present invention: the invention has the advantages that it has excellent disease-resistant performance, leaf is the key of plant growth, be photosynthetic main substance, here by direct leaf dressing, directly effect absorbs to the blade of crop fast, and employing liquid fermentation technology, thalline after utilizing ultrasonic wave to make fermentation after completing breaks Releasing nutrient and the cytotoxin of some remnants, antimicrobial component, effectively can prevent once the disease and pest of that degree, less insect pest incidence.

Embodiment

Insect pest foliar nutrient prepared by a kind of fermented liquid, it is characterized in that, be made up of the raw material of following weight part (Kg): rice bran 35, industrial corn powder 50, dry yellow leaf 12, flyash 6, dregs of beans 35, EM microbial inoculum 3, saturated limewater 12, urea 12, potassium primary phosphate 8, chinaberry extracting solution 5, zinc sulfate 3, precipitation agent 9 and appropriate water;

Described sinking agent is made up of the raw material of following weight part: lime powder 18,10% alum liquor 14,10% acetic acid solution 5, primary isoamyl alcohol 0.8, polyvinyl alcohol 7, tween 80 2, industrial Viscotrol C 3, ethanol 6 and water 50; The mixing of second alcohol and water is poured in reactor by preparation method, then polyvinyl alcohol is added, be heated to 70 ~ 80 DEG C of stirring and dissolving, then add 10% alum liquor, 10% acetic acid solution, primary isoamyl alcohol and lime powder, keep temperature of reaction kettle, adjustment rotating speed 150 ~ 200r/min stirs 20 ~ 35min, finally add tween 80 and industrial Viscotrol C simultaneously, insulated and stirred 30min is emulsion uniformly, obtains residue by funnel suction filtration, pulverizes to obtain pulvis sinking agent after oven dry.

A preparation method for insect pest foliar nutrient prepared by fermented liquid, is characterized in that, comprise following step:

(1) mixing rice bran, industrial corn powder, dry yellow leaf, flyash and dregs of beans, add the water of 10 ~ 12 times of its weight parts, ball milling 30 ~ 40min, to homogenate, pours in fermentor tank, carries out high-temperature sterilization process;

(2) EM microbial inoculum is added the water dilution of 5 times of its weight parts, be modulated into bacterium liquid, be then seeded in the fermentor tank of (1), adjustment pH5.5 ~ 7, temperature 25 ~ 28 DEG C, passes into sterile air simultaneously and stirs, and ferments 5 ~ 8 days;

(3) after (2) complete, fermented liquid is separately put in container, ultrasonic cell disruption instrument is utilized to carry out microbial disruption, with flowing water, container is lowered the temperature simultaneously, then precipitation agent mixing is added, then add saturated limewater, after stirring 8 ~ 10min, keep fermented liquid inertial motion, leave standstill 20 ~ 25min and carry out precipitation adsorption process voluntarily, after completing, supernatant liquor got by wooden dipper;

(4) mixing urea, potassium primary phosphate, chinaberry extracting solution, zinc sulfate and other remaining component, is added in the supernatant liquor described in (3), after stirring at room temperature is dissolved.

Insect pest foliar nutrient prepared by a kind of fermented liquid of the present invention is used for planting fertilizer viny, select 5 mu of grapevine fields as experimental group, indirect spray foliar nutrient of the present invention, a whole season 2 times, 5 mu of grapevines of control group use clear water or are left intact, other curing conditions and water and fertilizer management are all identical, result shows the grapevine of experimental group, and blade is more fresh and tender sturdy, and the incidence of disease and pest reduces, improve single cropping output, year economy raising 23.3%.

Claims (2)

1. the insect pest foliar nutrient prepared of a fermented liquid, it is characterized in that, be made up of the raw material of following weight part: rice bran 30 ~ 40, industrial corn powder 45 ~ 60, dry yellow leaf 10 ~ 15, flyash 5 ~ 8, dregs of beans 30 ~ 40, EM microbial inoculum 3 ~ 4, saturated limewater 10 ~ 15, urea 10 ~ 15, potassium primary phosphate 6 ~ 10, chinaberry extracting solution 3 ~ 7, zinc sulfate 2 ~ 4, precipitation agent 8 ~ 10 and appropriate water;
Described sinking agent is made up of the raw material of following weight part: lime powder 15 ~ 20,10% alum liquor 12 ~ 16,10% acetic acid solution 4 ~ 6, primary isoamyl alcohol 0.6 ~ 1.0, polyvinyl alcohol 6 ~ 8, tween 80 1.5 ~ 3, industrial Viscotrol C 2 ~ 4, ethanol 4 ~ 7 and water 50; The mixing of second alcohol and water is poured in reactor by preparation method, then polyvinyl alcohol is added, be heated to 70 ~ 80 DEG C of stirring and dissolving, then add 10% alum liquor, 10% acetic acid solution, primary isoamyl alcohol and lime powder, keep temperature of reaction kettle, adjustment rotating speed 150 ~ 200r/min stirs 20 ~ 35min, finally add tween 80 and industrial Viscotrol C simultaneously, insulated and stirred 30min is emulsion uniformly, obtains residue by funnel suction filtration, pulverizes to obtain pulvis sinking agent after oven dry.
2. the preparation method of insect pest foliar nutrient for preparing of a kind of fermented liquid according to claim 1, is characterized in that, comprise following step:
(1) mixing rice bran, industrial corn powder, dry yellow leaf, flyash and dregs of beans, add the water of 10 ~ 12 times of its weight parts, ball milling 30 ~ 40min, to homogenate, pours in fermentor tank, carries out high-temperature sterilization process;
(2) EM microbial inoculum is added the water dilution of 5 times of its weight parts, be modulated into bacterium liquid, be then seeded in the fermentor tank of (1), adjustment pH5.5 ~ 7, temperature 25 ~ 28 DEG C, passes into sterile air simultaneously and stirs, and ferments 5 ~ 8 days;
(3) after (2) complete, fermented liquid is separately put in container, ultrasonic cell disruption instrument is utilized to carry out microbial disruption, with flowing water, container is lowered the temperature simultaneously, then precipitation agent mixing is added, then add saturated limewater, after stirring 8 ~ 10min, keep fermented liquid inertial motion, leave standstill 20 ~ 25min and carry out precipitation adsorption process voluntarily, after completing, supernatant liquor got by wooden dipper;
(4) mixing urea, potassium primary phosphate, chinaberry extracting solution, zinc sulfate and other remaining component, is added in the supernatant liquor described in (3), after stirring at room temperature is dissolved.
CN201510470670.4A 2015-08-05 2015-08-05 Anti-insect pest foliar fertilizer prepared from fermentation liquor and preparation method for anti-insect pest foliar fertilizer CN105110900A (en)

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105418273A (en) * 2015-12-25 2016-03-23 常州大学 Preparation and application of rice straw biomass carbon-base release fertilizer
CN105967897A (en) * 2016-06-22 2016-09-28 望江乐和生态农业有限公司 Effect promoting agent for improving foliar fertilizer effect and preparation method of effect promoting agent
CN105985193A (en) * 2016-07-01 2016-10-05 安徽万利生态园林景观有限公司 Additive for enhancing foliage fertilizer efficiency and preparation method thereof
CN105985194A (en) * 2016-07-01 2016-10-05 安徽万利生态园林景观有限公司 Ginkgo foliage spray fertilizer additive and preparation method thereof
CN106116928A (en) * 2016-07-01 2016-11-16 安徽万利生态园林景观有限公司 A kind of pear tree foliar fertilizer promotees effect additive and preparation method thereof
CN106116902A (en) * 2016-07-01 2016-11-16 安徽万利生态园林景观有限公司 A kind of additive of mandarin orange foliage fertilizer and preparation method thereof
CN106116901A (en) * 2016-07-01 2016-11-16 安徽万利生态园林景观有限公司 A kind of additive promoting Folium Pini leaven effect and preparation method thereof
CN106187437A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 A kind of Rhizoma Solani tuber osi insect-pest foliar fertilizer and preparation method thereof
CN106187434A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 A kind of foliage fertilizer additives for ornamental trees and shrubs and preparation method thereof
CN106187436A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 A kind of osmanthus fragrans foliage fertilizer additives and preparation method thereof
CN106187435A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 Liquid fertilizer additive prepared by a kind of plant garbage and preparation method thereof
CN106187556A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 A kind of view lagerstroemia indica tree foliar fertilizer additive and preparation method thereof
CN106495840A (en) * 2016-10-11 2017-03-15 常州市鼎升环保科技有限公司 A kind of preparation method of the lasting foliage fertilizer of fertilizer efficiency
CN106699397A (en) * 2017-01-04 2017-05-24 桂林桂开生物科技股份有限公司 Organic fertilizer with insecticidal and sterilizing efficacy, and preparation method thereof

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JP2009167177A (en) * 2007-12-18 2009-07-30 Yoshimasa Kojima Plant protecting and activating agent
CN103787758A (en) * 2014-01-21 2014-05-14 枞阳县白云生态园林有限责任公司 Microbial organic compound fertilizer and preparation method for same
CN104045436A (en) * 2014-05-30 2014-09-17 青岛辰青信息技术有限公司 Foliar fertilizer preventing insect damage
CN104177195A (en) * 2014-09-11 2014-12-03 标优美生态工程股份有限公司 Special fertilizer for foliage spraying type flowers and preparation method thereof
CN104446701A (en) * 2014-11-19 2015-03-25 青岛高哲思服饰有限公司 Special organic fertilizer for tea trees and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009167177A (en) * 2007-12-18 2009-07-30 Yoshimasa Kojima Plant protecting and activating agent
CN103787758A (en) * 2014-01-21 2014-05-14 枞阳县白云生态园林有限责任公司 Microbial organic compound fertilizer and preparation method for same
CN104045436A (en) * 2014-05-30 2014-09-17 青岛辰青信息技术有限公司 Foliar fertilizer preventing insect damage
CN104177195A (en) * 2014-09-11 2014-12-03 标优美生态工程股份有限公司 Special fertilizer for foliage spraying type flowers and preparation method thereof
CN104446701A (en) * 2014-11-19 2015-03-25 青岛高哲思服饰有限公司 Special organic fertilizer for tea trees and preparation method thereof

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105418273A (en) * 2015-12-25 2016-03-23 常州大学 Preparation and application of rice straw biomass carbon-base release fertilizer
CN105967897A (en) * 2016-06-22 2016-09-28 望江乐和生态农业有限公司 Effect promoting agent for improving foliar fertilizer effect and preparation method of effect promoting agent
CN106187437A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 A kind of Rhizoma Solani tuber osi insect-pest foliar fertilizer and preparation method thereof
CN105985194A (en) * 2016-07-01 2016-10-05 安徽万利生态园林景观有限公司 Ginkgo foliage spray fertilizer additive and preparation method thereof
CN106116928A (en) * 2016-07-01 2016-11-16 安徽万利生态园林景观有限公司 A kind of pear tree foliar fertilizer promotees effect additive and preparation method thereof
CN106116902A (en) * 2016-07-01 2016-11-16 安徽万利生态园林景观有限公司 A kind of additive of mandarin orange foliage fertilizer and preparation method thereof
CN106116901A (en) * 2016-07-01 2016-11-16 安徽万利生态园林景观有限公司 A kind of additive promoting Folium Pini leaven effect and preparation method thereof
CN105985193A (en) * 2016-07-01 2016-10-05 安徽万利生态园林景观有限公司 Additive for enhancing foliage fertilizer efficiency and preparation method thereof
CN106187434A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 A kind of foliage fertilizer additives for ornamental trees and shrubs and preparation method thereof
CN106187436A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 A kind of osmanthus fragrans foliage fertilizer additives and preparation method thereof
CN106187435A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 Liquid fertilizer additive prepared by a kind of plant garbage and preparation method thereof
CN106187556A (en) * 2016-07-01 2016-12-07 安徽万利生态园林景观有限公司 A kind of view lagerstroemia indica tree foliar fertilizer additive and preparation method thereof
CN106495840A (en) * 2016-10-11 2017-03-15 常州市鼎升环保科技有限公司 A kind of preparation method of the lasting foliage fertilizer of fertilizer efficiency
CN106699397A (en) * 2017-01-04 2017-05-24 桂林桂开生物科技股份有限公司 Organic fertilizer with insecticidal and sterilizing efficacy, and preparation method thereof

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