CN104692925A - Agricultural microbial ecological agent for soil conditioning and plant calcium supplement and production method thereof - Google Patents
Agricultural microbial ecological agent for soil conditioning and plant calcium supplement and production method thereof Download PDFInfo
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- CN104692925A CN104692925A CN201510057280.4A CN201510057280A CN104692925A CN 104692925 A CN104692925 A CN 104692925A CN 201510057280 A CN201510057280 A CN 201510057280A CN 104692925 A CN104692925 A CN 104692925A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/005—Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The invention relates to the field of an agricultural microbial ecological agent, and particularly relates to an agricultural microbial ecological agent for soil conditioning and plant calcium supplement and a production method thereof. The agent is prepared according to the following steps: with oyster shell powder as a basic raw material and molasses meal as a nutrient medium, at first, fermenting an oyster shell by using zymophyte, and then adding other useful formula bacteria according to a reasonable proportion to prepare the agricultural microbial ecological agent. The agent not only can recycle waste materials to solve environmental pollution, but also can achieve large-scale high-value development and utilization of oyster shells, greatly improve the yield of plants and provide an economic and efficient agricultural microbial ecological agent for the development of modern organic agriculture.
Description
Technical field
The present invention relates to microbiobacterial agent field, be specifically related to a kind of agriculture probiotics and production method thereof, particularly a kind of agriculture probiotics of replenishing the calcium for soil conditioning and plant and production method thereof.
Background technology
China is large agricultural country, and be also that chemical fertilizer maximum in the world uses state, fertilizer application amount accounts for 1/3rd of global consumption.Simple dependence chemical fertilizer realizes crop yield and has become more and more difficult at present.On the contrary, owing to excessively using chemical fertilizer to cause soil acidification, the soil salinization, soil compaction, soil microorganisms serious unbalance continuously, soil fertility declines, and quality of agricultural product declines, and soil-borne disease, and hunger sign in plant, Plant diseases is on the rise.Development like this is gone down, and prospect is troubling.Therefore, soil conditioning and soil in-situ repair imperative.
Main containing mineral substance calcium carbonate in oyster shells, also containing medium trace element and amino acid, organic matters etc. such as magnesium, copper, iron, zinc, manganese, strontiums.Because wherein calcium carbonate content is very high, itself is again in weakly alkaline, and on the one hand for improvement shortage calcium carbonate with in acid soil, effect is comparatively obvious.Another aspect is again that a kind of well calcium source supplements for the crop such as fruit tree, vegetables, thus the disease that prevention causes because of plant calcium deficiency is well lost.But oyster shellss a large amount of is at present abandoned by as rubbish, both wastes resource, causes environmental pollution again.More do not meet agricultural sustainable development strategy.Owing to being limited to the idea of people and current scientific and technological level, agriculturally also insufficient to the exploitation of oyster shells at present, just tip of the iceberg, be even in the starting stage, the product developed also is primary products.Such as: use as soil conditioner with after calcination method process oyster shells, method is comparatively simple, product is also more single.
Microbial bacteria has conditioning soil, in-situ immobilization, eliminate soil compaction, impel farm crop well developed root system, regulation and control plant growth, strengthen crop disease prevention resistance against diseases, improve crops quality and output, and the advantage such as free from environmental pollution, so, microbe application is significant in agricultural, but, China develops relatively slow in the agricultural application of microbial technique, and one is because scientific research strength is weak, in the bacterial screening of microorganism, domestication and fermentation technique etc., lack breakthrough.Two is that application disconnects especially with scientific research, lacks application data accurately, makes to apply job development delayed.
Therefore; be necessary to use microbial fermentation technology; oyster shells is carried out biological treatment processing; obtain a kind of product innovation; in soil conditioning and plant are replenished the calcium, all can reach good effect, turn waste into wealth, protection of the environment; the demand of modern agricultural production can be met again simultaneously, there is boundless development prospect.
Summary of the invention
For many weak points that prior art exists, the invention provides a kind of agriculture probiotics of replenishing the calcium for soil conditioning and plant and production method thereof, this probiotics take oyster shells as main component, obtain after mixing with formula bacterium after zymophyte fermentation, achieve the high-valued recycling of oyster shells, both soil can be nursed one's health after using, rehabilitating soil, plant root can be realized again replenish the calcium, plant is made to improve defending and fighting against diseases ability, substantially increase the output of plant simultaneously, there is cost low, effective, easy to use, safety, green, nuisanceless, the efficient of modern agricultural production, economical product.
Concrete technical scheme of the present invention is:
A kind of agriculture probiotics, its main active principle is counted by weight:
Oyster shell powder 90-95 part, molasses powder 2-4 part, water 2-4 part, zymophyte 0.5-1 part, formula bacterium 0.5-1 part;
Described zymophyte is the mixture of Lactobacterium acidophilum and bifidus bacillus, and its mass ratio is: 55-70:30-45;
Described formula bacterium comprises subtilis, plant lactobacillus, bifidus bacillus, lactobacillus rhamnosus, bacillus megaterium, Bacillus licheniformis; Its mass ratio is: 25-35:5-15:5-15:15-25:5-15:5-15;
Described oyster shell powder fineness more than 100 orders, to facilitate follow-up processing.
Contriver selects oyster shell powder to be basic raw material, major cause is the calcium carbonate having high content in oyster shells, also have medium trace element and amino acid, the organic matters etc. such as copper, iron, zinc, manganese, magnesium, these compositions can provide sufficient mineral substance and other nutrition for plant.The existence of these compositions simultaneously both can nurse one's health Soil structure, took full advantage of waste again; The calcium carbonate simultaneously wherein contained easily is fermented bacterium fermentation for organic calcium, the absorption of convenient plant; In order to reach better effect, the weight percent of the preferred major ingredient of the oyster shells selected by the present invention is: calcium carbonate 95%, and magnesium, copper, iron, zinc, manganese, strontium etc. 3%, the oyster shell powder of amino acid 2%, can play better effect like this.
Above-mentioned formula bacterium is selected to join in probiotics, can rehabilitating soil, elimination is hardened, and promotes that crop root is flourishing, and regulation and control plant growth, strengthens resistance against diseases, improves the effect of crop quality; Achieve efficient, safety, the green of this probiotics, environmental protection, pollution-free.
And molasses powder is the source of nutrition as bacterial classification in above-mentioned recipe ingredient, major cause is because it is with low cost and nutritious, at utmost can reduce production cost, conveniently be easy to get.
First difference of the present invention and prior art is: first adopt zymophyte that oyster shell powder is carried out biological fermentation, containing Lactobacterium acidophilum, bifidus bacillus in zymophyte.Wherein said Lactobacillus acidophilus species is numbered ACCC00155, bifidobacterium species is numbered ACCC11054.Above-mentioned bacterial classification all directly can be obtained by existing approach, and contriver does not repeat them here.Why select above-mentioned bacterial classification, major cause is as follows:
Described Lactobacterium acidophilum can secrete the lactic acid with extremely strong sterilizing ability, and by the physiological activity of self bacterial strain, the lactic acid of secretion makes raw material acidifying, and calcium carbonate has been changed into calcium lactate, calcium acetate, thus forms the form being easy to be utilized by soil and plant.
Described bifidus bacillus thalline secretion has lactic acid, acetic acid, the acetic acid of extremely strong sterilizing ability, by the physiological activity of self bacterial strain, calcium carbonate has been changed into calcium lactate, calcium acetate by lactic acid, the acetic acid of secretion, and amino acid calcium, calglucon form state that can be absorbed by crops.
Contriver is with Lactobacterium acidophilum and bifidus bacillus, with the nutritive substance of molasses powder as bacterium, oyster shell powder is carried out biological fermentation, time is 10-15 days, control pressure 0.2-0.5MPa during fermentation, temperature 28-30 DEG C, pH6-7, wherein the organic calciums (ionized calcium) such as calcium lactate, calcium acetate, amino acid calcium, calglucon will have been changed into by most of inorganic calcium (calcium carbonate) through above-mentioned fermentation, crop root directly can be absorbed, and move up along with the moisture transpiration effect done in object; Obviously can increase calcium ion Ca in soil on the other hand
2+content, effectively alleviates the acidity of soil, is conducive to recovering soil aggregate, keeps soil fertility and fertility; During fermentation, remaining thalline can continue secretion lactic acid etc. simultaneously, plays the effect of soil sterilization.
Second difference of the present invention and prior art is after the oyster shells that fermented, more useful formula bacterium is fully mixed obtained finished product with it, namely have employed the formula bacterium of accurate proportioning as effective ingredient.Containing subtilis, plant lactobacillus, bifidus bacillus, lactobacillus rhamnosus, bacillus megaterium, Bacillus licheniformis in formula bacterium, wherein said Bacillus subtilis strain is numbered ACCC10242, plant lactobacillus bacterium numbering is ACCC10171, bifidobacterium species is numbered ACCC11054, lactobacillus rhamnosus bacterium numbering is ACCC10534, bacillus megaterium bacterium numbering is ACCC01667, and Bacillus licheniformis strain is numbered ACCC19372.
Above-mentioned bacterial classification all directly can be obtained by existing approach, and contriver does not repeat them here.Why select above-mentioned bacterial classification, major cause is as follows:
The subtilyne that can produce in described subtilis thalli growth process, polymyxin, nystatin, linear gramicidins isoreactivity material, the conditioned pathogen of these active substances to pathogenic bacterium or autogenous infection has obvious restraining effect; The free oxygen in environment can be consumed rapidly simultaneously, and promote the growth of milk-acid bacteria, produce the organic acids such as lactic acid, indirectly suppress the growth of other pathogenic bacterium; The enzymes such as thalline self synthesis α-amylase, proteolytic enzyme, lipase, cellulase, are conducive to the growth of plant;
Described plant lactobacillus can secrete lactic acid, has the effect promoting Calcium-ion absorption, also has the lactic acid of extremely strong sterilizing ability simultaneously, and harmful microbe can be suppressed to breed and activity, also can accelerate the decomposition of organic substance.Accelerate absorption and the conversion of calcium ion;
Described bifidus bacillus can secrete lactic acid, acetic acid, has the effect promoting and also have protective plant vascular bundle path while the effect of protection Calcium-ion absorption, accelerates dredging of the effective nutrition of plant;
Described lactobacillus rhamnosus can secrete lactic acid, promotes Calcium-ion absorption, reduces conditioning soil while fixing;
Plant lactobacillus, bifidus bacillus, lactobacillus rhamnosus acting in conjunction protection calcium ion are not solidified by other chemical ion, thus promote effective absorption of crop;
Described bacillus megaterium can improve fertilizer efficiency, by invalid phosphorus is converted into available phosphorus, effectively improves the specific absorption of Soil Phosphorus, alleviate disease while degraded medicine is residual, can control nutrition and other resources of rhizosphere, causes pathogenic bacteria to reduce living space and condition; Make that plant related organization cell walls thickens, fibrosis, degree of lignification improve, and form the two silicon layer of cutin outward at epidermal area, form the barrier stoping germ invasion and attack together;
The flora imbalance of described Bacillus licheniformis adjustable reaches therapeutic purpose, and body can be impelled to produce antibacterial substance, kill pathogenic bacterium; There is unique biology and take oxygen mechanism of action by force, suppress the growth and breeding of pathogenic bacterium; Go back decomposable asymmetric choice net hazardous and noxious substances, purification soil simultaneously; It has stronger proteolytic enzyme, lipase, diastatic activity simultaneously, and its proteolytic enzyme produced can regulate soil acidity or alkalinity, and can produce various plants growth hormone, promotes plant growth; There is the ability of very strong degraded plant and animal residues; Improve the soil organism, improvement soil; Consume oxygen simultaneously, promote the growth of the plant lactobacillus of anaerobism, bifidus bacillus and rhamnosyl milk-acid bacteria, while reaching conditioning soil, effectively promote the absorption of calcium ion.
Above-mentioned each bacterium fits together by contriver, determine its concrete species and application percentage, there is the good effect that has complementary functions to each other, and owing to have employed the nutritive substance of molasses powder as bacterium, advantageously in the Growth and reproduction of above-mentioned each bacterium, thus play better effect.
Contriver, through further research, determines that its main best active principle is counted by weight:
Oyster shell powder 93 parts, 2 parts, molasses powder, 3 parts, water, zymophyte 1 part, formula bacterium 1 part;
Wherein said zymophyte is the mixture of Lactobacterium acidophilum and bifidus bacillus, and its mass ratio is: 65:35;
Described formula bacterium comprises subtilis, plant lactobacillus, bifidus bacillus, lactobacillus rhamnosus, bacillus megaterium, Bacillus licheniformis; Its mass ratio is: 30:15:12:20:11:12.
It is high that the agriculture probiotics that final the present invention obtains has organic calcium (ionized calcium) content, the gemma rate of microbial bacteria is high, it is fast, effective to bring back to life, conditioning Soil structure, improve soil productivity, remove fertilizer damage poisoning, promote that crop takes root, promote that crop is replenished the calcium, improve resistance against diseases, improve crop quality, the advantage such as increase crop yield.
Another inventive point of the present invention then there is provided the production method of this agriculture probiotics, concrete steps are as follows: be first broken into by oyster shell powder Powdered, fineness is more than 100 orders, then in proportion the molasses powder of zymophyte, half, water are sprayed equably on oyster shell powder, and be uniformly mixed, sealing secondary fermentation 10 ~ 15 days, control pressure 0.2-0.5MPa during fermentation, temperature 28-30 DEG C, pH6-7; After having fermented, mix equably with formula bacterium and remaining molasses powder more in proportion, get final product packaging final prod.
The invention has the beneficial effects as follows:
1, agriculture probiotics of the present invention adopts oyster shells to be basic raw material, applying biological fermentation technique carries out modification to it, and the principle superposed according to function with microbial bacteria reasonably combined obtained by, the effect such as there is outstanding soil conditioning, in-situ immobilization, crop is replenished the calcium, diseases prevention is degeneration-resistant; Pollution-free, nuisanceless, environment-friendly and green, safe and efficient, easy to use, mix fertile bottom application, ditch spread, spread manuer in holes, achieve the resource circulation utilization of discarded oyster shells, and oyster shells cost is low, its medium trace element and aminoacids content enrich, and can meet the requirement of the Sustainable development of modern agriculture.
2, promote the formation of soil aggregate, improve soil physical property
Inorganic calcium in oyster shells changes into organic calcium after biological fermentation, and wherein electronegative soil particle coagulation can well get up by the organic active calcium ion of positively charged, forms good crumb structure, makes nutrient not easily leaching loss;
Multiple-microorganism bacterium wherein be also formed soil aggregate enliven factor.One is because thread fungus can be wound around grogs with mycelia form coacervate; Two is because the polysaccharide material of microbial bacteria metabolism in vital movement and some organic polymer have good cementation to grogs; Three is that microorganism passes through to promote the formation of soil ulmin and participates in being formed soil aggregation thing;
Experiment proves: after using probiotics of the present invention, define good soil aggregate, soil becomes more loose, ventilation, more be conducive to that beneficial microorganism in the growth of root system of plant and soil is movable, existence, breeding, and microorganism amount reproduction does not cause the original microbial ecosystem of soil to change yet.
3, soil acidity or alkalinity is regulated
Probiotics of the present invention contains abundant organic active calcium substance, medium trace element and microorganism, and itself is in weakly alkaline, and the acid in energy sweetening of the soil, makes soil pH value raise, effectively alleviate the acidity of soil, is conducive to recovering soil productivity.
4, soil water retention property is improved
Oyster shells is after biological fermentation, itself have nano level multi-cellular structure, can adsorb moisture and the nutrient of some amount, calcium ion is with positive charge, can by the Water Molecular Adsorption of polarity on oneself surface, after being manured into soil, if soil moisture is on the high side, probiotics will absorb excessive moisture, by its temporary storage, when crop needs moisture, then moisture is discharged, play a kind of effect of intelligent control.
5, the ability that crop is replenished the calcium is improved
Calcium is a kind of component of crop cell wall, and during calcium deficiency, the formation of cell walls is hindered, and plant growth is suppressed, and time serious, crop organ can be festered necrosis, has at least more than 20 kind of physiological disease to be caused by calcium deficiency in crop; Probiotics of the present invention is the organic calcium produced after biological fermentation, at soil and do in object, bacterium moves with water, calcium moves with bacterium, interior raw symbiosis bacterium arrives each position of crop making to carry in the growth in object, breeding, moving process calcium ion, thus realize replenishing the calcium to crop, the effectively generation of prevention crop multiple diseases.
6, crop disease prevention anti-adversity ability is improved
The genus bacillus that probiotics of the present invention adopts have gemma rate high, bring back to life fast, breed the advantages such as fast, dominant microflora can be formed fast after using, thus effectively suppress the breeding of harmful levels of pathogens, reach with the antibacterial object of bacterium, prevention crop root disease effect is very remarkable; In addition, beneficial microorganism bacterium can promote crop root growth, and main root is healthy and strong, and side root is flourishing, and capillary root enriches, and Crop nutrition fast comprehensively and continually, plant growth is healthy and strong, and diseases prevention anti-adversity ability all can strengthen.
7, improve utilization rate of fertilizer, improve crop yield and quality
Probiotics of the present invention can significantly improve crop after application and absorb the decomposition of organic matter, the absorption rate of inorganic fertilizer is made to improve about 10%, crop is except can increasing production, disease-resistant degeneration-resistant, cold-resistant drought-resistant ability all can improve, and dry matter content and the SOD content of crop can be improved, extend collection period, storage period, extend quality guarantee period and the shelf-lives of crop.
8, in probiotics of the present invention living bacteria count can reach 2-7 hundred million/gram.
9, the raw material sources of probiotics of the present invention is extensive, with low cost, and can produce on a large scale.Product cost is low, effective, security is high, fermentation period is short, preparation technology is simple and convenient, suitable universal and popularization.
Embodiment
Further illustrate the present invention below in conjunction with embodiment, those skilled in the art can be made more fully to understand the present invention, but do not limit the present invention in any way.
Embodiment 1
For the agriculture probiotics that soil conditioning and plant are replenished the calcium, its main active principle is counted by weight: oyster shell powder 93 parts, 2 parts, molasses powder, 3 parts, water, zymophyte 1 part, formula bacterium 1 part.
In described oyster shell powder, the weight percent of major ingredient is: calcium carbonate 95%, magnesium, copper, iron, zinc, manganese, strontium etc. 3%, amino acid 2%.Being crushed to fineness is that 100 order left and right are for subsequent use.
Described zymophyte is Lactobacterium acidophilum and bifidus bacillus, and its mass ratio is: 60:40, and wherein said Lactobacillus acidophilus species is numbered ACCC00155, bifidobacterium species is numbered ACCC11054;
Described formula bacterium comprises subtilis, plant lactobacillus, bifidus bacillus, lactobacillus rhamnosus, bacillus megaterium, Bacillus licheniformis, its mass ratio is: 30:10:10:20:9:9, described Bacillus subtilis strain is numbered ACCC10242, plant lactobacillus bacterium numbering is ACCC10171, bifidobacterium species is numbered ACCC11054, lactobacillus rhamnosus bacterium numbering is ACCC10534, bacillus megaterium bacterium numbering is ACCC01667, and Bacillus licheniformis strain is numbered ACCC19372.
The production method of above-mentioned agriculture probiotics, concrete steps are as follows: be first broken into by oyster shell powder Powdered, fineness is more than 100 orders, then in proportion zymophyte and half molasses powder and water are sprayed equably on oyster shell powder, and be uniformly mixed, sealing secondary fermentation 10 ~ 15 days, control pressure 0.2-0.5MPa during fermentation, temperature 28-30 DEG C, pH6-7.After having fermented, mix equably with formula bacterium and half molasses powder more in proportion, get final product packaging final prod.
Embodiment 2
For the agriculture probiotics that soil conditioning and plant are replenished the calcium, its main active principle is counted by weight: oyster shell powder 91 parts, 4 parts, molasses powder, 4 parts, water, zymophyte 0.5 part, formula bacterium 0.5 part.
In described oyster shell powder, the weight percent of major ingredient is: calcium carbonate 94.5%, and magnesium, copper, iron, zinc, manganese, strontium etc. 3.6%, amino acid/11 .9%, being crushed to fineness is that 100 order left and right are for subsequent use.
Described zymophyte is Lactobacterium acidophilum and bifidus bacillus, and its mass ratio is: 55:45, and wherein said Lactobacillus acidophilus species is numbered ACCC00155, and bifidobacterium species is numbered ACCC11054;
Described formula bacterium comprises subtilis, plant lactobacillus, bifidus bacillus, lactobacillus rhamnosus, bacillus megaterium, Bacillus licheniformis, its mass ratio is: 27:7:7:17:6:6, described Bacillus subtilis strain is numbered ACCC10242, plant lactobacillus bacterium numbering is ACCC10171, bifidobacterium species is numbered ACCC11054, lactobacillus rhamnosus bacterium numbering is ACCC10534, bacillus megaterium bacterium numbering is ACCC01667, and Bacillus licheniformis strain is numbered ACCC19372.
The production method of above-mentioned agriculture probiotics, concrete steps are as follows: be first broken into by oyster shell powder Powdered, fineness is more than 100 orders, then in proportion zymophyte and half molasses powder and water are sprayed equably on oyster shell powder, and be uniformly mixed, sealing secondary fermentation 10 ~ 15 days, control pressure 0.2-0.5MPa during fermentation, temperature 28-30 DEG C, pH6-7.After having fermented, mix equably with formula bacterium and half molasses powder more in proportion, get final product packaging final prod.
Embodiment 3
For the agriculture probiotics that soil conditioning and plant are replenished the calcium, its main active principle is counted by weight: oyster shell powder 94.5 parts, 2 parts, molasses powder, 2 parts, water, zymophyte 0.75 part, formula bacterium 0.75 part.
In described oyster shell powder, the weight percent of major ingredient is: calcium carbonate 94.7%, magnesium, copper, iron, zinc, manganese, strontium etc. 3.5%, amino acid/11 .8%.Being crushed to fineness is that 100 order left and right are for subsequent use.
Described zymophyte is Lactobacterium acidophilum and bifidus bacillus, and its mass ratio is: 70:30, and wherein said Lactobacillus acidophilus species is numbered ACCC00155, and bifidobacterium species is numbered ACCC11054;
Described formula bacterium comprises subtilis, plant lactobacillus, bifidus bacillus, lactobacillus rhamnosus, bacillus megaterium, Bacillus licheniformis, its mass ratio is: 32:12:11:21:10:10, described Bacillus subtilis strain is numbered ACCC10242, plant lactobacillus bacterium numbering is ACCC10171, bifidobacterium species is numbered ACCC11054, lactobacillus rhamnosus bacterium numbering is ACCC10534, bacillus megaterium bacterium numbering is ACCC01667, and Bacillus licheniformis strain is numbered ACCC19372.
The production method of above-mentioned agriculture probiotics, concrete steps are as follows: be first broken into by oyster shell powder Powdered, fineness is more than 100 orders, then in proportion zymophyte and half molasses powder and water are sprayed equably on oyster shell powder, and be uniformly mixed, sealing secondary fermentation 10 ~ 15 days, control pressure 0.2-0.5MPa during fermentation, temperature 28-30 DEG C, pH6-7.After having fermented, mix equably with formula bacterium and half molasses powder more in proportion, get final product packaging final prod.
Experimental example
Experimental example 1
Experiment crop: garlic
Experimental products: the probiotics described in embodiment 1
Experimental measuring: 10 kgs/acre
Using method: bottom application, mixes together with other fertilizer (fertilizer, chemical fertilizer).
Experimental result: compare with blank group, experimental group is emerged early 1 day, and Miao Qimiao strengthens, and root system is flourishing especially.Have obvious improving effect (acidity alleviates) to soil, not there is the diseases such as anthrax in growing period, and growing way is vigorous, and every mu is increased production 265 jin, volume increase 11.2%.
Experimental example 2
Experiment crop: apple
Experimental products: the probiotics described in embodiment 1
Experimental measuring: 500 grams of/trees
Using method: bottom application, when spring topdresses and other fertilizer mix together.
Experimental result: compare with blank group, experimental group is comparatively strong to " cold spell in later spring " defensive ability/resistance ability occurred spring, and florescence and blade show good stress resistance.Vegetative period, apple disease obviously alleviated, and loosing soil situation is good, and capillary root increases, and limb is vigorous, leaf dark green, and vein is clear, fruit light, and size is impartial, and the apple proportion of 90mm is high by 10.5%, and pol increases, and mouthfeel improves, and volume increase reaches 12%.
Experimental example 3
Experiment crop: tomato
Experimental products: the probiotics described in embodiment 2
Experimental measuring: 20 kgs/acre
Using method: bottom application, mixes together with other fertilizer.
Experimental result: compare with blank group, the neat seedling of experimental group seedling is strengthened, and root system is flourishing especially, and capillary root is many, and stem spacing is shorter, leaf dark green, and vein is clear, blooms early, and early, the collecting period in advance, and extends the collecting period to setting, fruit light, and storage tolerance is good, not easily sends out wrinkle and rots.All have good preventive effect to blossom-end rot, root rot, verticillium, early late blight, stimulation ratio reaches 14.7%.Obvious to the moss removal effect on native top layer, loosing soil is good, and soil acidity alleviates.
Experimental example 4
Experiment crop: cherry
Experimental products: the probiotics described in embodiment 2
Experimental measuring: 500 grams of/trees
Using method: bottom application, mixes together with other fertilizer.
Experimental result: compare with blank group, experimental group resists " cold spell in later spring " successful occurred spring, fully can promote normal development and the elongation of pollen bud and pollen tube, significantly improve fruit-setting rate, reduces flowers and fruits and comes off.Vegetative period, disease obviously alleviated, and loosing soil situation is good, and capillary root increases, and limb is vigorous, leaf dark green, and vein is clear, fruit light, and size is impartial, and pol increases, and mouthfeel improves, and storage tolerance is good, and volume increase reaches 20%.
Experimental example 5
Experiment crop: grape
Experimental products: the probiotics described in embodiment 3
Experimental measuring: 20 kgs/acre
Using method: bottom application, mixes together with other fertilizer.
Experimental result: compare with blank group, experimental group is at new slightly growth and florescence, and development of floral organs is abundant, obviously reduces incomplete flower, reduces coming off of flower, improves fruit-setting rate.Leaf dark green, growing way is good, and photosynthetic rate is high, facilitate fruit grain early expand, early painted, early ripe, pol improves, and mouthfeel is good, and volume increase reaches 15%, and the shelf lives can extend 3-4 days.Whole vegetative period, disease obviously alleviated, and loosing soil situation improves, and root growth is good, and capillary root is many, and has delayed leaf senile, postponed fallen leaves.
As can be seen here, agriculture probiotics provided by the invention achieves the high-valued recycling of oyster shells, both soil, rehabilitating soil can be nursed one's health after using, plant root can be realized again replenish the calcium, plant is made to improve defending and fighting against diseases ability, substantially increasing the output of plant simultaneously, have cost low, effective, easy to use, safe, green, nuisanceless, is efficient, the economical product of modern agricultural production.
Claims (7)
1. for the agriculture probiotics that soil conditioning and plant are replenished the calcium, it is characterized in that: its main active principle is counted by weight:
Oyster shell powder 90-95 part, molasses powder 2-4 part, water 2-4 part, zymophyte 0.5-1 part, formula bacterium 0.5-1 part.
2. agriculture probiotics according to claim 1, is characterized in that: described zymophyte is the mixture of Lactobacterium acidophilum and bifidus bacillus, and its mass ratio is: 55-70:30-45.
3. agriculture probiotics according to claim 1, is characterized in that: described formula bacterium comprises subtilis, plant lactobacillus, bifidus bacillus, lactobacillus rhamnosus, bacillus megaterium, Bacillus licheniformis; Its mass ratio is: 25-35:5-15:5-15:15-25:5-15:5-15.
4. agriculture probiotics according to claim 1, is characterized in that: described oyster shell powder fineness is more than 100 orders.
5. the agriculture probiotics according to claim 1 or 2 or 3, is characterized in that: its main active principle is counted by weight: oyster shell powder 93 parts, 2 parts, molasses powder, 3 parts, water, zymophyte 1 part, formula bacterium 1 part;
Wherein said zymophyte is the mixture of Lactobacterium acidophilum and bifidus bacillus, and its mass ratio is: 65:35;
Described formula bacterium comprises subtilis, plant lactobacillus, bifidus bacillus, lactobacillus rhamnosus, bacillus megaterium, Bacillus licheniformis; Its mass ratio is: 30:15:12:20:11:12.
6. agriculture probiotics according to claim 5, is characterized in that:
In described zymophyte, Lactobacillus acidophilus species is numbered ACCC00155, and bifidobacterium species is numbered ACCC11054;
In described formula bacterium, Bacillus subtilis strain is numbered ACCC10242, plant lactobacillus bacterium numbering is ACCC10171, bifidobacterium species is numbered ACCC11054, lactobacillus rhamnosus bacterium numbering is ACCC10534, bacillus megaterium bacterium numbering is ACCC01667, and Bacillus licheniformis strain is numbered ACCC19372.
7. the production method of agriculture probiotics described in claim 1, it is characterized in that: concrete steps are as follows: first oyster shell powder is broken into Powdered, fineness is more than 100 orders, then in proportion zymophyte and half molasses powder and whole water are sprayed equably on oyster shell powder, and be uniformly mixed, sealing secondary fermentation 10 ~ 15 days, control pressure 0.2-0.5MPa during fermentation, temperature 28-30 DEG C, pH6-7;
After having fermented, mix equably with formula bacterium and remaining molasses powder more in proportion, get final product packaging final prod.
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