CN113249262A - Compound microbial agent for preventing and treating diseases and application thereof - Google Patents
Compound microbial agent for preventing and treating diseases and application thereof Download PDFInfo
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- CN113249262A CN113249262A CN202110597708.XA CN202110597708A CN113249262A CN 113249262 A CN113249262 A CN 113249262A CN 202110597708 A CN202110597708 A CN 202110597708A CN 113249262 A CN113249262 A CN 113249262A
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- 201000010099 disease Diseases 0.000 title claims abstract description 42
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 42
- 230000000813 microbial effect Effects 0.000 title claims abstract description 34
- 150000001875 compounds Chemical class 0.000 title claims abstract description 29
- 229920001661 Chitosan Polymers 0.000 claims abstract description 77
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical class O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims abstract description 57
- 239000003381 stabilizer Substances 0.000 claims abstract description 43
- 239000011573 trace mineral Substances 0.000 claims abstract description 43
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 43
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 31
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 31
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 31
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims abstract description 31
- 241000187747 Streptomyces Species 0.000 claims abstract description 31
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000011591 potassium Substances 0.000 claims abstract description 29
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 29
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims abstract description 27
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 27
- 239000007633 bacillus mucilaginosus Substances 0.000 claims abstract description 27
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 27
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 22
- 241000186660 Lactobacillus Species 0.000 claims abstract description 16
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 16
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 56
- 238000002156 mixing Methods 0.000 claims description 44
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 30
- 241000894006 Bacteria Species 0.000 claims description 28
- 239000004310 lactic acid Substances 0.000 claims description 28
- 235000014655 lactic acid Nutrition 0.000 claims description 28
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 22
- 239000000243 solution Substances 0.000 claims description 21
- 238000001035 drying Methods 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 13
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 239000002994 raw material Substances 0.000 claims description 12
- 238000005303 weighing Methods 0.000 claims description 12
- 229910021538 borax Inorganic materials 0.000 claims description 11
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 11
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 11
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 11
- 239000011790 ferrous sulphate Substances 0.000 claims description 11
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 11
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 11
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 11
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 11
- 229940099596 manganese sulfate Drugs 0.000 claims description 11
- 235000007079 manganese sulphate Nutrition 0.000 claims description 11
- 239000011702 manganese sulphate Substances 0.000 claims description 11
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 11
- 239000004328 sodium tetraborate Substances 0.000 claims description 11
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 11
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 11
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 11
- 229960001763 zinc sulfate Drugs 0.000 claims description 11
- 244000199866 Lactobacillus casei Species 0.000 claims description 10
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 239000000440 bentonite Substances 0.000 claims description 10
- 229910000278 bentonite Inorganic materials 0.000 claims description 10
- 238000004090 dissolution Methods 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 229940017800 lactobacillus casei Drugs 0.000 claims description 10
- 241000186016 Bifidobacterium bifidum Species 0.000 claims description 9
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 8
- 239000011975 tartaric acid Substances 0.000 claims description 8
- 235000002906 tartaric acid Nutrition 0.000 claims description 8
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 5
- 239000004334 sorbic acid Substances 0.000 claims description 5
- 235000010199 sorbic acid Nutrition 0.000 claims description 5
- 229940075582 sorbic acid Drugs 0.000 claims description 5
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 4
- 244000057717 Streptococcus lactis Species 0.000 claims description 4
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 4
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 4
- 235000015165 citric acid Nutrition 0.000 claims description 4
- 239000001630 malic acid Substances 0.000 claims description 4
- 235000011090 malic acid Nutrition 0.000 claims description 4
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 3
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims 6
- 239000002689 soil Substances 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 12
- 241000196324 Embryophyta Species 0.000 abstract description 7
- 230000002265 prevention Effects 0.000 abstract description 7
- 239000002068 microbial inoculum Substances 0.000 abstract description 6
- 208000035143 Bacterial infection Diseases 0.000 abstract description 4
- 208000031888 Mycoses Diseases 0.000 abstract description 4
- 238000012271 agricultural production Methods 0.000 abstract description 4
- 239000002131 composite material Substances 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 4
- 239000003895 organic fertilizer Substances 0.000 abstract description 4
- 230000035699 permeability Effects 0.000 abstract description 4
- 230000003612 virological effect Effects 0.000 abstract description 4
- 230000000052 comparative effect Effects 0.000 description 11
- 238000003973 irrigation Methods 0.000 description 8
- 230000002262 irrigation Effects 0.000 description 8
- 239000000575 pesticide Substances 0.000 description 7
- 235000002566 Capsicum Nutrition 0.000 description 3
- 235000016623 Fragaria vesca Nutrition 0.000 description 3
- 240000009088 Fragaria x ananassa Species 0.000 description 3
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 3
- 239000006002 Pepper Substances 0.000 description 3
- 241000722363 Piper Species 0.000 description 3
- 235000016761 Piper aduncum Nutrition 0.000 description 3
- 235000017804 Piper guineense Nutrition 0.000 description 3
- 235000008184 Piper nigrum Nutrition 0.000 description 3
- 239000013043 chemical agent Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a compound microbial agent for preventing and treating diseases and application thereof, relating to the technical field of microbial agents and comprising the following components: the compound microbial agent is prepared from bacillus subtilis, bacillus licheniformis, rhodobacter palustris, bacillus mucilaginosus, bifidobacterium, phanerochaete chrysosporium, lactobacillus, streptomyces jingyangensis, a stabilizer, chitosan modified bentonite, trace elements and potassium fulvate, can effectively prevent and treat plant root diseases, has a very outstanding prevention and treatment effect, and can be widely applied to agricultural production; the bio-organic fertilizer prepared by the composite microbial inoculum can condition soil, activate the activity rate of microorganisms in the soil, overcome soil hardening, increase air permeability of the soil, prevent and treat various fungal diseases and bacterial diseases and inhibit viral diseases.
Description
Technical Field
The invention relates to the technical field of microbial agents, in particular to a compound microbial agent for preventing and treating diseases and application thereof.
Background
Plant diseases are one of the important factors for restricting the high quality and high yield of crops. It is estimated that the average loss of major crops worldwide amounts to about 10-15% of the total yield, with direct economic losses of up to billions of dollars per year. Plant diseases not only directly cause the reduction of crop yield and quality, but also can secrete and generate a plurality of toxins and metabolites harmful to human and livestock in the process of infecting crops by partial pathogenic bacteria, thereby forming a great threat to the safety of agricultural products. Among various diseases, the root diseases of crops are always the headache which troubles farmers, and the occurrence of the root diseases can directly cause the problems of serious seedling shortage, seedling death, short seedling, uneven seedling emergence and the like, directly influences the crop yield and causes great economic loss.
At present, the prevention and the treatment of diseases are mainly based on chemical agents, and the chemical agents have the advantage of good quick-acting property. However, the long-term use of chemical pesticide has not only the killing and poisoning effect on pests, but also the large amount of chemical pesticide, which causes the pesticide to gradually accumulate in the environment, especially in the soil, and causes the problem of pesticide pollution to the environment. Prevention of pesticide contamination has become a concern in many countries of the world today. The use of pesticides is closely related to agriculture, forestry, animal husbandry and the like, and therefore, the pollution of the pesticides to soil is one of important environmental problems.
Disclosure of Invention
The invention aims to provide a compound microbial agent for preventing and treating diseases and application thereof, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a compound microbial agent for preventing and treating diseases comprises the following components in parts by weight:
4-12 parts of bacillus subtilis, 2-6 parts of bacillus licheniformis, 1-5 parts of rhodobacter palustris, 3-7 parts of bacillus mucilaginosus, 5-9 parts of bifidobacterium, 1-4 parts of phanerochaete chrysosporium, 6-11 parts of lactic acid bacteria, 1-4 parts of streptomyces jingyangensis, 2-4 parts of a stabilizer, 7-15 parts of chitosan modified bentonite, 0.5-2 parts of trace elements and 50-60 parts of potassium fulvate.
As a further scheme of the invention: the compound microbial agent for preventing and treating diseases comprises the following components in parts by weight:
6-10 parts of bacillus subtilis, 3-5 parts of bacillus licheniformis, 2-4 parts of rhodobacter palustris, 4-6 parts of bacillus mucilaginosus, 6-8 parts of bifidobacterium, 2-3 parts of phanerochaete chrysosporium, 8-10 parts of lactic acid bacteria, 2-3 parts of streptomyces jingyangensis, 3-4 parts of a stabilizer, 9-12 parts of chitosan modified bentonite, 1-1.5 parts of trace elements and 53-57 parts of potassium fulvate.
As a still further scheme of the invention: the compound microbial agent for preventing and treating diseases comprises the following components in parts by weight:
8 parts of bacillus subtilis, 4 parts of bacillus licheniformis, 3 parts of rhodobacter palustris, 5 parts of bacillus mucilaginosus, 7 parts of bifidobacterium, 3 parts of phanerochaete chrysosporium, 9 parts of lactic acid bacteria, 3 parts of streptomyces jingyang, 4 parts of a stabilizer, 10 parts of chitosan modified bentonite, 1 part of trace element and 55 parts of potassium fulvate.
As a still further scheme of the invention: the lactobacillus is one or more of lactobacillus plantarum, streptococcus lactis and lactobacillus casei.
As a still further scheme of the invention: the stabilizer is one or more of malic acid, citric acid, tartaric acid and sorbic acid.
As a still further scheme of the invention: the trace elements mainly comprise the following raw materials, by weight, 1-2 parts of zinc sulfate, 2-3 parts of borax, 0.2-0.4 part of manganese sulfate, 0.3-0.5 part of copper sulfate, 0.02-0.06 part of magnesium sulfate and 0.05-0.1 part of ferrous sulfate.
As a still further scheme of the invention: the preparation method of the chitosan modified bentonite comprises the following steps: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution and 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished.
A preparation method of a compound microbial agent for preventing and treating diseases comprises the following steps:
1) weighing 4-12 parts of bacillus subtilis, 2-6 parts of bacillus licheniformis, 1-5 parts of rhodobacter palustris, 3-7 parts of bacillus mucilaginosus, 5-9 parts of bifidobacterium, 1-4 parts of phanerochaete chrysosporium, 6-11 parts of lactic acid bacteria, 1-4 parts of streptomyces jingyangensis, 2-4 parts of a stabilizer, 7-15 parts of chitosan modified bentonite and 0.5-2 parts of trace elements in sequence according to the weight part ratio;
2) mixing chitosan modified bentonite with Bacillus subtilis, Bacillus licheniformis, Rhodobacillus palustris, Bacillus mucilaginosus, Bacillus bifidus, Phanerochaete chrysosporium, lactobacillus and Streptomyces jingyangensis uniformly;
3) adding a stabilizer, trace elements and potassium fulvate into the mixture obtained in the step 2), and mixing again to obtain the compound microbial agent for preventing and treating diseases.
The compound microbial agent for preventing and treating diseases is applied to preparing fertilizers.
Compared with the prior art, the invention has the beneficial effects that:
1) the microbial agent prepared by the invention can effectively prevent and treat plant root diseases, has very outstanding prevention and treatment effects, and can be widely applied to agricultural production;
2) the bio-organic fertilizer prepared by the composite microbial inoculum can condition soil, activate the activity rate of microorganisms in the soil, overcome soil hardening, increase air permeability of the soil, prevent and treat various fungal diseases and bacterial diseases and inhibit viral diseases;
3) pesticide residues and macromolecular organic matters can be effectively degraded; the disease resistance and immunity of crops are enhanced; the yield of crops is improved; the quality of the product is effectively ensured.
Detailed Description
The microbial agent prepared by the invention can effectively prevent and treat plant root diseases, has very outstanding prevention and treatment effects, and can be widely applied to agricultural production; the bio-organic fertilizer prepared by the composite microbial inoculum can condition soil, activate the activity rate of microorganisms in the soil, overcome soil hardening, increase air permeability of the soil, prevent and treat various fungal diseases and bacterial diseases and inhibit viral diseases.
The technical solution of the present invention will be described in further detail with reference to specific embodiments.
Example 1
Weighing 4 parts of bacillus subtilis, 2 parts of bacillus licheniformis, 1 part of rhodobacter palustris, 3 parts of bacillus mucilaginosus, 5 parts of bifidobacterium, 1 part of phanerochaete chrysosporium, 6 parts of lactic acid bacteria, 1 part of streptomyces jingyang, 2 parts of stabilizing agent, 7 parts of chitosan modified bentonite, 0.5 part of trace elements and 50 parts of potassium fulvate in sequence according to the parts by weight, wherein the lactic acid bacteria are plant lactobacillus, the stabilizing agent comprises malic acid, the trace elements mainly comprise the following raw materials in parts by weight, zinc sulfate 1 part, borax 2 parts, manganese sulfate 0.2 part, copper sulfate 0.3 part, magnesium sulfate 0.02 part and ferrous sulfate 0.05 part, and the preparation method of the chitosan modified bentonite comprises the following steps: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution with 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished; mixing chitosan modified bentonite with Bacillus subtilis, Bacillus licheniformis, Rhodobacillus palustris, Bacillus mucilaginosus, Bacillus bifidus, Phanerochaete chrysosporium, lactobacillus and Streptomyces jingyangensis uniformly; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the compound microbial agent for preventing and treating diseases.
Example 2
Weighing 6 parts of bacillus subtilis, 3 parts of bacillus licheniformis, 2 parts of rhodobacter palustris, 4 parts of bacillus mucilaginosus, 6 parts of bifidobacterium, 2 parts of phanerochaete chrysosporium, 7 parts of lactic acid bacteria, 2 parts of streptomyces jingyang, 3 parts of stabilizing agent, 8 parts of chitosan modified bentonite, 0.8 part of trace elements and 52 parts of potassium fulvate in sequence according to the parts by weight, wherein the lactic acid bacteria are streptococcus lactis, the stabilizing agent comprises citric acid, the trace elements mainly comprise the following raw materials in parts by weight, zinc sulfate is 1.5 parts, borax is 2.5 parts, manganese sulfate is 0.3 part, copper sulfate is 0.4 part, magnesium sulfate is 0.03 part, and ferrous sulfate is 0.06 part, and the preparation method of the chitosan modified bentonite comprises the following steps: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution with 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished; mixing chitosan modified bentonite with Bacillus subtilis, Bacillus licheniformis, Rhodobacillus palustris, Bacillus mucilaginosus, Bacillus bifidus, Phanerochaete chrysosporium, lactobacillus and Streptomyces jingyangensis uniformly; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the compound microbial agent for preventing and treating diseases.
Example 3
Weighing 8 parts of bacillus subtilis, 4 parts of bacillus licheniformis, 3 parts of rhodobacter palustris, 5 parts of bacillus mucilaginosus, 7 parts of bifidobacterium, 3 parts of phanerochaete chrysosporium, 8 parts of lactic acid bacteria, 3 parts of streptomyces jingyang, 4 parts of stabilizing agent, 10 parts of chitosan modified bentonite, 1 part of trace elements and 53 parts of potassium fulvate in sequence according to the parts by weight, wherein the lactic acid bacteria is lactobacillus casei, the stabilizing agent comprises tartaric acid, the trace elements mainly comprise the following raw materials in parts by weight, and the preparation method of the chitosan modified bentonite comprises the following steps of: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution with 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished; mixing chitosan modified bentonite with Bacillus subtilis, Bacillus licheniformis, Rhodobacillus palustris, Bacillus mucilaginosus, Bacillus bifidus, Phanerochaete chrysosporium, lactobacillus and Streptomyces jingyangensis uniformly; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the compound microbial agent for preventing and treating diseases.
Example 4
Weighing 9 parts of bacillus subtilis, 5 parts of bacillus licheniformis, 4 parts of rhodobacter palustris, 6 parts of bacillus mucilaginosus, 8 parts of bifidobacterium, 4 parts of phanerochaete chrysosporium, 9 parts of lactic acid bacteria, 4 parts of streptomyces jingyang, 2 parts of stabilizing agent, 12 parts of chitosan modified bentonite, 1.2 parts of trace elements and 55 parts of potassium fulvate in sequence according to the parts by weight, wherein the lactic acid bacteria are lactobacillus casei, the stabilizing agent comprises tartaric acid, the trace elements mainly comprise the following raw materials in parts by weight, zinc sulfate 1.5 parts, borax 2 parts, manganese sulfate 0.3 part, copper sulfate 0.4 part, magnesium sulfate 0.05 part and ferrous sulfate 0.08 part, and the preparation method of the chitosan modified bentonite comprises the following steps: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution with 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished; mixing chitosan modified bentonite with Bacillus subtilis, Bacillus licheniformis, Rhodobacillus palustris, Bacillus mucilaginosus, Bacillus bifidus, Phanerochaete chrysosporium, lactobacillus and Streptomyces jingyangensis uniformly; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the compound microbial agent for preventing and treating diseases.
Example 5
Weighing 10 parts of bacillus subtilis, 6 parts of bacillus licheniformis, 5 parts of rhodobacter palustris, 7 parts of bacillus mucilaginosus, 9 parts of bifidobacterium, 2 parts of phanerochaete chrysosporium, 10 parts of lactic acid bacteria, 2 parts of streptomyces jingyang, 3 parts of stabilizing agent, 13 parts of chitosan modified bentonite, 1.5 parts of trace elements and 57 parts of potassium fulvate in sequence according to the parts by weight, wherein the lactic acid bacteria are lactobacillus casei, the stabilizing agent comprises sorbic acid, the trace elements mainly comprise the following raw materials in parts by weight, zinc sulfate 1 part, borax 3 parts, manganese sulfate 0.2 part, copper sulfate 0.5 part, magnesium sulfate 0.03 part and ferrous sulfate 0.1 part, and the preparation method of the chitosan modified bentonite comprises the following steps: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution with 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished; mixing chitosan modified bentonite with Bacillus subtilis, Bacillus licheniformis, Rhodobacillus palustris, Bacillus mucilaginosus, Bacillus bifidus, Phanerochaete chrysosporium, lactobacillus and Streptomyces jingyangensis uniformly; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the compound microbial agent for preventing and treating diseases.
Example 6
Weighing 11 parts of bacillus subtilis, 4 parts of bacillus licheniformis, 3 parts of rhodobacter palustris, 4 parts of bacillus mucilaginosus, 6 parts of bifidobacterium, 3 parts of phanerochaete chrysosporium, 8 parts of lactic acid bacteria, 3 parts of streptomyces jingyang, 4 parts of stabilizing agent, 14 parts of chitosan modified bentonite, 1 part of trace elements and 60 parts of potassium fulvate in sequence according to the parts by weight, wherein the lactic acid bacteria is lactobacillus casei, the stabilizing agent comprises sorbic acid, the trace elements mainly comprise the following raw materials in parts by weight, zinc sulfate is 1.5 parts, borax is 2 parts, manganese sulfate is 0.4 part, copper sulfate is 0.4 part, magnesium sulfate is 0.06 part, and ferrous sulfate is 0.08 part, and the preparation method of the chitosan modified bentonite comprises the following steps: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution with 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished; mixing chitosan modified bentonite with Bacillus subtilis, Bacillus licheniformis, Rhodobacillus palustris, Bacillus mucilaginosus, Bacillus bifidus, Phanerochaete chrysosporium, lactobacillus and Streptomyces jingyangensis uniformly; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the compound microbial agent for preventing and treating diseases.
Example 7
Weighing 12 parts of bacillus subtilis, 6 parts of bacillus licheniformis, 5 parts of rhodobacter palustris, 7 parts of bacillus mucilaginosus, 9 parts of bifidobacterium, 4 parts of phanerochaete chrysosporium, 11 parts of lactic acid bacteria, 4 parts of streptomyces jingyang, 4 parts of stabilizing agent, 15 parts of chitosan modified bentonite, 2 parts of trace elements and 60 parts of potassium fulvate in sequence according to the weight parts, the lactobacillus is a composition of lactobacillus plantarum, streptococcus lactis and lactobacillus casei, the combination ratio is 1:1:1, the stabilizer is a composition of malic acid, citric acid, tartaric acid and sorbic acid, the combination ratio is 1:1:1:1, the trace elements mainly comprise the following raw materials in parts by weight, 1.5 parts of zinc sulfate, 2 parts of borax, 0.4 part of manganese sulfate, 0.4 part of copper sulfate, 0.06 part of magnesium sulfate and 0.08 part of ferrous sulfate, and the preparation method of the chitosan modified bentonite comprises the following steps: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution with 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished; mixing chitosan modified bentonite with Bacillus subtilis, Bacillus licheniformis, Rhodobacillus palustris, Bacillus mucilaginosus, Bacillus bifidus, Phanerochaete chrysosporium, lactobacillus and Streptomyces jingyangensis uniformly; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the compound microbial agent for preventing and treating diseases.
Comparative example 1
Weighing 9 parts of bacillus subtilis, 5 parts of bacillus licheniformis, 4 parts of rhodobacter palustris, 6 parts of bacillus mucilaginosus, 8 parts of bifidobacterium, 4 parts of phanerochaete chrysosporium, 9 parts of lactic acid bacteria, 4 parts of streptomyces jingyang, 2 parts of a stabilizer, 1.2 parts of trace elements and 55 parts of potassium fulvate in sequence according to the weight part ratio, wherein the lactic acid bacteria is lactobacillus casei, the stabilizer comprises tartaric acid, and the trace elements mainly comprise the following raw materials in parts by weight, namely 1.5 parts of zinc sulfate, 2 parts of borax, 0.3 part of manganese sulfate, 0.4 part of copper sulfate, 0.05 part of magnesium sulfate and 0.08 part of ferrous sulfate; uniformly mixing bacillus subtilis, bacillus licheniformis, rhodobacter palustris, bacillus mucilaginosus, bifidobacterium, phanerochaete chrysosporium, lactobacillus and streptomyces jingyangensis; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the finished product.
Comparative example 2
The preparation method comprises the following steps of weighing 9 parts of bacillus subtilis, 5 parts of bacillus licheniformis, 8 parts of bifidobacterium, 4 parts of phanerochaete chrysosporium, 9 parts of lactic acid bacteria, 4 parts of streptomyces jingyangensis, 2 parts of a stabilizer, 12 parts of chitosan modified bentonite, 1.2 parts of trace elements and 55 parts of potassium fulvate in sequence according to the parts by weight, wherein the lactic acid bacteria are lactobacillus casei, the stabilizer comprises tartaric acid, the trace elements mainly comprise the following raw materials in parts by weight, 1.5 parts of zinc sulfate, 2 parts of borax, 0.3 part of manganese sulfate, 0.4 part of copper sulfate, 0.05 part of magnesium sulfate and 0.08 part of ferrous sulfate, and the preparation method of the chitosan modified bentonite comprises the following steps: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution with 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished; uniformly mixing the chitosan modified bentonite with bacillus subtilis, bacillus licheniformis, bifidobacterium, phanerochaete chrysosporium, lactobacillus and streptomyces jingyangensis; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the finished product.
Comparative example 3
Weighing 9 parts of bacillus subtilis, 5 parts of bacillus licheniformis, 8 parts of bifidobacterium, 4 parts of phanerochaete chrysosporium, 9 parts of lactic acid bacteria, 4 parts of streptomyces jingyangensis, 2 parts of a stabilizer, 1.2 parts of trace elements and 55 parts of potassium fulvate in sequence according to the weight part ratio, wherein the lactic acid bacteria is lactobacillus casei, the stabilizer comprises tartaric acid, and the trace elements mainly comprise the following raw materials in parts by weight, namely 1.5 parts of zinc sulfate, 2 parts of borax, 0.3 part of manganese sulfate, 0.4 part of copper sulfate, 0.05 part of magnesium sulfate and 0.08 part of ferrous sulfate; uniformly mixing bacillus subtilis, bacillus licheniformis, bifidobacterium, phanerochaete chrysosporium, lactobacillus and streptomyces jingyangensis; and adding a stabilizer, trace elements and potassium fulvate into the obtained mixture, and mixing again to obtain the finished product.
Experimental example 1
The crop variety is pepper, the microbial inoculum prepared in examples 1-7 and comparative examples 1-3 is used for root irrigation of pepper at the early stage of the onset of pepper bacterial wilt, the using amount is 15 g/mu, the root irrigation is performed for 1 time every 4d, the root irrigation is performed for 3 times totally, after the root irrigation is performed for 5d for the last time, the control effect is calculated, no treatment is performed on a control group, and the result is shown in the following table 1:
| group of | Control effect (%) |
| Example 1 | 86.3 |
| Example 2 | 84.9 |
| Example 3 | 85.2 |
| Example 4 | 87.5 |
| Example 5 | 84.1 |
| Example 6 | 86.7 |
| Example 7 | 85.4 |
| Comparative example 1 | 42.3 |
| Comparative example 2 | 48.6 |
| Comparative example 3 | 32.1 |
| Control group | 5.9 |
Experimental example 2
The crop variety is strawberry, the microbial inoculum prepared in examples 1-7 and comparative examples 1-3 is used for root irrigation of the strawberry at the early stage of the root rot of the strawberry, the using amount is 20 g/mu, the root irrigation is carried out for 1 time every 3d, the root irrigation is carried out for 3 times totally, after the root irrigation is carried out for the last 1 time and 5d, the prevention and control effect is calculated, no treatment is carried out on a control group, and the structure is shown in table 2:
| group of | Control effect (%) |
| Example 1 | 86.8 |
| Example 2 | 84.6 |
| Example 3 | 86.4 |
| Example 4 | 87.1 |
| Example 5 | 85.2 |
| Example 6 | 86.1 |
| Example 7 | 87.3 |
| Comparative example 1 | 39.4 |
| Comparative example 2 | 42.1 |
| Comparative example 3 | 21.4 |
| Control group | 2.6 |
In conclusion, the microbial agent prepared by the invention can effectively prevent and treat plant root diseases, has very outstanding prevention and treatment effects, and can be widely applied to agricultural production; the bio-organic fertilizer prepared by the composite microbial inoculum can condition soil, activate the activity rate of microorganisms in the soil, overcome soil hardening, increase air permeability of the soil, prevent and treat various fungal diseases and bacterial diseases and inhibit viral diseases.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (9)
1. The compound microbial agent for preventing and treating diseases is characterized by comprising the following components in parts by weight:
4-12 parts of bacillus subtilis, 2-6 parts of bacillus licheniformis, 1-5 parts of rhodobacter palustris, 3-7 parts of bacillus mucilaginosus, 5-9 parts of bifidobacterium, 1-4 parts of phanerochaete chrysosporium, 6-11 parts of lactic acid bacteria, 1-4 parts of streptomyces jingyangensis, 2-4 parts of a stabilizer, 7-15 parts of chitosan modified bentonite, 0.5-2 parts of trace elements and 50-60 parts of potassium fulvate.
2. The compound microbial inoculant for controlling diseases according to claim 1, which comprises the following components in parts by weight:
6-10 parts of bacillus subtilis, 3-5 parts of bacillus licheniformis, 2-4 parts of rhodobacter palustris, 4-6 parts of bacillus mucilaginosus, 6-8 parts of bifidobacterium, 2-3 parts of phanerochaete chrysosporium, 8-10 parts of lactic acid bacteria, 2-3 parts of streptomyces jingyangensis, 3-4 parts of a stabilizer, 9-12 parts of chitosan modified bentonite, 1-1.5 parts of trace elements and 53-57 parts of potassium fulvate.
3. The compound microbial inoculant for preventing and treating diseases as claimed in claim 1 or 2, which comprises the following components in parts by weight:
8 parts of bacillus subtilis, 4 parts of bacillus licheniformis, 3 parts of rhodobacter palustris, 5 parts of bacillus mucilaginosus, 7 parts of bifidobacterium, 3 parts of phanerochaete chrysosporium, 9 parts of lactic acid bacteria, 3 parts of streptomyces jingyang, 4 parts of a stabilizer, 10 parts of chitosan modified bentonite, 1 part of trace element and 55 parts of potassium fulvate.
4. The compound microbial agent for controlling diseases according to claim 3, wherein the lactic acid bacteria is one or more of Lactobacillus plantarum, Streptococcus lactis and Lactobacillus casei.
5. The compound microbial inoculant for controlling diseases according to claim 4, wherein the stabilizer is one or more of malic acid, citric acid, tartaric acid and sorbic acid.
6. The compound microbial inoculant for preventing and treating diseases as claimed in claim 5, wherein the trace elements mainly comprise raw materials in parts by weight of 1-2 parts of zinc sulfate, 2-3 parts of borax, 0.2-0.4 part of manganese sulfate, 0.3-0.5 part of copper sulfate, 0.02-0.06 part of magnesium sulfate and 0.05-0.1 part of ferrous sulfate.
7. The compound microbial inoculant for controlling diseases according to claim 6, wherein the preparation method of the chitosan modified bentonite comprises the following steps: dissolving 5 parts of chitosan by using 100 parts of acetic acid aqueous solution with volume fraction of 5%, obtaining a chitosan solution after the dissolution is finished, mixing the chitosan solution and 60 parts of bentonite, blending into uniform paste, placing the paste in a microwave oven with power of 700W for heating and drying, and obtaining the chitosan modified bentonite after the drying is finished.
8. A method for preparing a compound microbial agent for controlling diseases according to any one of claims 1 to 7, which comprises the following steps:
1) weighing 4-12 parts of bacillus subtilis, 2-6 parts of bacillus licheniformis, 1-5 parts of rhodobacter palustris, 3-7 parts of bacillus mucilaginosus, 5-9 parts of bifidobacterium, 1-4 parts of phanerochaete chrysosporium, 6-11 parts of lactic acid bacteria, 1-4 parts of streptomyces jingyangensis, 2-4 parts of a stabilizer, 7-15 parts of chitosan modified bentonite and 0.5-2 parts of trace elements in sequence according to the weight part ratio;
2) mixing chitosan modified bentonite with Bacillus subtilis, Bacillus licheniformis, Rhodobacillus palustris, Bacillus mucilaginosus, Bacillus bifidus, Phanerochaete chrysosporium, lactobacillus and Streptomyces jingyangensis uniformly;
3) adding a stabilizer, trace elements and potassium fulvate into the mixture obtained in the step 2), and mixing again to obtain the compound microbial agent for preventing and treating diseases.
9. The use of a compound microbial inoculant for controlling diseases as claimed in any one of claims 1 to 7 in the preparation of a fertilizer.
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