CN105111014A - Foliar fertilizer prepared from high-nutrition fermentation broth and preparation method thereof - Google Patents
Foliar fertilizer prepared from high-nutrition fermentation broth and preparation method thereof Download PDFInfo
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- CN105111014A CN105111014A CN201510471291.7A CN201510471291A CN105111014A CN 105111014 A CN105111014 A CN 105111014A CN 201510471291 A CN201510471291 A CN 201510471291A CN 105111014 A CN105111014 A CN 105111014A
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Abstract
The invention discloses foliar fertilizer prepared from a high-nutrition fermentation broth and a preparation method thereof. The foliar fertilizer is prepared from the following raw materials in parts by weight: grape residues, edible fungus residues, an EM microbial agent, saturated lime water, urea, monoammonium phosphate, earthworm soil, poultry feathers, chinaberry fruits, rapeseed residues, precipitant and a proper amount of water. By adopting a liquid state fermentation method, fermentation can be complete and thoroughly, a fermented product is rich, the foliar fertilizer can be used for giving crops high-nutritional supply, which is naturally fermented liquid-state nutrition, can be absorbed more easily and is healthier. By adopting the foliar fertilizer, waste recycling can be realized, resources can be saved, and the environment is protected.
Description
Technical field
The invention belongs to the Technology field of fertilizer, body relates to foliar nutrient that a kind of high nutritious fermentation liquor produces and preparation method thereof.
Background technology
Liquid fermenting is one of modern biotechnology, it refers in biochemical reactor, natural imitation circle is by the oxygen of edible medicinal fungus in residue process required for necessary carbohydrate, organic and inorganic some trace elements of compound, inorganic salt etc. containing nitrogen and other thalline respiratory metabolism, and control suitable ambient conditions, carry out the prolific process of mycelia.The large-scale fermentation culture of industrialization is fermentative production, also known as deep drainpipe or reticent cultivation.Industrial fermentation is produced must adopt fermentor tank, and fermentation culture many employings triangular flask in laboratory.Contain thalline in the fermented liquid obtained, be decomposed and undecomposed nutritive ingredient, the meta-bolites that thalline produces.The direct hyoscine of fermented liquid or confession separation and Extraction, also can do liquid spawn.
The energy needed for the liquid of edible medicinal fungus can use cerelose, industrial starch and Rhizoma Dioscoreae powder etc.; Nitrogenous source can adopt soybean cake powder, dried silkworm chrysalis meal, bran powder etc.In order to reduce costs, usually also adopt part trade effluent to be surrogate, as soybean wastewater, raw material sources are extensive.The nutrition distribution pattern of liquid nutrient medium is even, is conducive to abundant contact and the absorption of mushroom nourishing body.Mycelia can grow, can discharge in time the metabolism waste gas of respiration generation inside and outside reactor under optimum temperuture, pH, oxygen and carbon-nitrogen ratio condition, therefore metabolic vigorous, mycelial growth division rapidly, the meta-bolites that a large amount of mycelium and polysaccharide, polypeptide etc. have physiologically active can be accumulated at short notice, cultivated by liquid state fermentation and obtain a large amount of mycelium and physiologically active substance and generally only need the time of 2 ~ 7 days and cell age is neat, and solid culture needs 30 ~ 50 days.Liquid strain can effectively reduce strain pollution rate, has flowing fast, easily disperses, a bacterium point is many, sprout the features such as fast, can effectively reduce the pollution of edible mushrooms in seeded process.
The product of microbial metabolism is broadly divided into two classes: primary metabolite and secondary metabolite, primary metabolite is that the breeding of microbe metabolism growth is necessary, can produce in whole life cycle more always, amino acid, Nucleotide, polysaccharide, lipid and VITAMIN etc. are produced in cell, and secondary metabolite microbiotic, alkaloid, bacteriotoxin and plant growth factor etc. that to be extracellular produce, of a great variety.
Summary of the invention
The present invention, in order to improve the quality of foliar nutrient, improves the utilization ratio of liquid fertilizer, and after inventing a kind of liquid state fermentation, the effective constituent extracted in fermented liquid makes a kind of high efficiency composition liquid fertilizer, and specific practice is as follows:
The foliar nutrient that a kind of high nutritious fermentation liquor is produced, it is characterized in that, be made up of the raw material of following weight part: trester 15 ~ 20, edible fungi residue 45 ~ 60, EM microbial inoculum 2 ~ 3, saturated limewater 10 ~ 15, urea 7 ~ 10, monoammonium phosphate 6 ~ 10, earthworm soil 15 ~ 25, poultry feather 10 ~ 15, chinaberry fruit 20 ~ 30, vegetable seed slag 50 ~ 60, precipitation agent 8 ~ 10 and appropriate water;
Described sinking agent is made up of the raw material of following weight part: lime powder 15 ~ 20,10% alum liquor 12 ~ 16,10% acetic acid solution 4 ~ 6, primary isoamyl alcohol 0.6 ~ 1.0, polyvinyl alcohol 6 ~ 8, tween 80 1.5 ~ 3, industrial Viscotrol C 2 ~ 4, ethanol 4 ~ 7 and water 50; The mixing of second alcohol and water is poured in reactor by preparation method, then polyvinyl alcohol is added, be heated to 70 ~ 80 DEG C of stirring and dissolving, then add 10% alum liquor, 10% acetic acid solution, primary isoamyl alcohol and lime powder, keep temperature of reaction kettle, adjustment rotating speed 150 ~ 200r/min stirs 20 ~ 35min, finally add tween 80 and industrial Viscotrol C simultaneously, insulated and stirred 30min is emulsion uniformly, obtains residue by funnel suction filtration, pulverizes to obtain pulvis sinking agent after oven dry.
A preparation method for the foliar nutrient that high nutritious fermentation liquor is produced, is characterized in that, comprises following step:
(1) mix trester, edible fungi residue, earthworm soil, poultry feather, chinaberry fruit and vegetable seed slag, add the water of 10 ~ 15 times of its weight parts, ball milling 30 ~ 40min, to homogenate, pours in fermentor tank, carries out high-temperature sterilization process;
(2) EM microbial inoculum is added the water dilution of 5 times of its weight parts, be modulated into bacterium liquid, be then seeded in the fermentor tank of (1), adjustment pH5.5 ~ 7, temperature 25 ~ 28 DEG C, passes into sterile air simultaneously and stirs, and ferments 5 ~ 8 days;
(3) after (2) complete, fermented liquid is separately put in container, ultrasonic cell disruption instrument is utilized to carry out microbial disruption, with flowing water, container is lowered the temperature simultaneously, then precipitation agent mixing is added, add saturated limewater again, after stirring 8 ~ 10min, keep fermented liquid inertial motion, leave standstill 20 ~ 25min and carry out precipitation adsorption process voluntarily, supernatant liquor is obtained after completing;
(4) mixing urea, monoammonium phosphate and other remaining component, be added in the supernatant liquor described in (3), stirring at room temperature 45 ~ 60min adsorbs, after completing.
By alum, (main component is KAl (SO
4)
2gain enlightenment, its principle utilizes it in water, ionize out two metal ion species, and Al
3+be easy to hydrolysis, generate the aluminium hydroxide Al of colloid
3++ 3H
2o=Al (OH)
3+ 3H
+(reversible), the adsorptive power of alumine hydroxide colloid is strong, the impurity that can suspend in planar water, and form precipitation, make clarification of water, although successful, but the throw out after sedimentation is easily loose, be again diffused in water, the present invention not only will make the fermented liquid after fermentation clarify, also must possess make not allow composition granule composition to condense speed is fast, settling velocity fast, easily extract the features such as supernatant liquor after sedimentation, the present invention proposes sinking agent for this reason.
The operation instruction of sinking agent: adopt 10% alum liquor to be the main component of precipitation agent, the sedimentation effect reached is best, then 10% acetic acid is adopted to have certain promoter action as the separating effect of sedimentation medium to inorganic sinking agent, but the reaction of acetic acid and fermented liquid is comparatively slow, period, some fermented liquid was overflowed with the bubble of densification, so add primary isoamyl alcohol when preparing sinking agent as defoamer, adding the minimum lime powder of particle diameter is stablizer, secondly the weight of sorbent material is increased, avoid the sinking agent settling velocity of one-component slow, adding polyvinyl alcohol makes each ingredient adsorption of Tai-Ace S 150 and sedimentation promoter action on the surface of lime powder particle as binding agent, after all the components has adsorbed, finally adding a certain amount of vegetables oil can effectively prevent sinking agent particle to be mutually sticked together, become agglomerate, influence effect, particle aggregation agent after completing just can with an overall form storage, transport and use, successful.
The present invention adopts liquid state fermentation to ferment, first initial centrifugation, the oarse-grained particulate matter of filtering, ultrasonic cell disintegration instrument is utilized by remaining liq to carry out fragmentation to the microorganism in liquid, simultaneously in order to prevent heat production in process, cause temperature to raise and adopt flowing water stream to lower the temperature, by special sinking agent, adsorption precipitation is carried out to the residue in fermentation and unfermentable solid waste, obtain comparatively pure liquid extracting solution, some mineral substance that add that finally can be composite carry out function improvement and nutritional supplementation, it is reduce molecule to the impact of blade that pure extracting solution is used as foliar nutrient, the easy blocking blades pore of finely ground particles, affect respiration, next is unfavorable for prevention and elimination of disease and pests.
Intracellular organic matter can be discharged: the organic compositions such as amino acid, Nucleotide, nucleic acid, carbohydrate, make the nutritive ingredient of extracting solution abundanter while the meaning of microbial disruption is microorganism cells cracking.
Beneficial effect of the present invention: adopt the method for liquid state fermentation, make to ferment more completely, more thorough, the more horn of plenty of the product after fermentation; here raw material is made up of the most dregs of fat and bacterium slag; nutritious in raw material, the quality of product, also relative to more various and comprehensive, does the supply of object height nutrition for the preparation of foliar nutrient; from natural fermented liquid nutritional; relatively be more prone to absorb, more healthy, the huge profit realizing refuse is used; saving resource, protection of the environment.
Embodiment
Embodiment 1:
The foliar nutrient that a kind of high nutritious fermentation liquor is produced, it is characterized in that, be made up of the raw material of following weight part (Kg): trester 20, edible fungi residue 45, EM microbial inoculum 2, saturated limewater 15, urea 7, monoammonium phosphate 8, earthworm soil 20, poultry feather 12, chinaberry fruit 22, vegetable seed slag 60, precipitation agent 9 and appropriate water;
Described sinking agent is made up of the raw material of following weight part: lime powder 16,10% alum liquor 12,10% acetic acid solution 5, primary isoamyl alcohol 0.8, polyvinyl alcohol 7, tween 80 2.2, industrial Viscotrol C 3, ethanol 5 and water 50; The mixing of second alcohol and water is poured in reactor by preparation method, then polyvinyl alcohol is added, be heated to 70 ~ 80 DEG C of stirring and dissolving, then add 10% alum liquor, 10% acetic acid solution, primary isoamyl alcohol and lime powder, keep temperature of reaction kettle, adjustment rotating speed 150 ~ 200r/min stirs 20 ~ 35min, finally add tween 80 and industrial Viscotrol C simultaneously, insulated and stirred 30min is emulsion uniformly, obtains residue by funnel suction filtration, pulverizes to obtain pulvis sinking agent after oven dry.
A preparation method for the foliar nutrient that high nutritious fermentation liquor is produced, is characterized in that, comprises following step:
(1) mix trester, edible fungi residue, earthworm soil, poultry feather, chinaberry fruit and vegetable seed slag, add the water of 10 ~ 15 times of its weight parts, ball milling 30 ~ 40min, to homogenate, pours in fermentor tank, carries out high-temperature sterilization process;
(2) EM microbial inoculum is added the water dilution of 5 times of its weight parts, be modulated into bacterium liquid, be then seeded in the fermentor tank of (1), adjustment pH5.5 ~ 7, temperature 25 ~ 28 DEG C, passes into sterile air simultaneously and stirs, and ferments 5 ~ 8 days;
(3) after (2) complete, fermented liquid is separately put in container, ultrasonic cell disruption instrument is utilized to carry out microbial disruption, with flowing water, container is lowered the temperature simultaneously, then precipitation agent mixing is added, add saturated limewater again, after stirring 8 ~ 10min, keep fermented liquid inertial motion, leave standstill 20 ~ 25min and carry out precipitation adsorption process voluntarily, supernatant liquor is obtained after completing;
(4) mixing urea, monoammonium phosphate and other remaining component, be added in the supernatant liquor described in (3), stirring at room temperature 45 ~ 60min adsorbs, after completing.
Select 5 mu of apple fields, the foliar nutrient that a kind of high nutritious fermentation liquor of the present invention is produced is sprayed on the blade of apple tree with every mu of usage quantity 80 ~ 120Kg, spray 4 ~ 6 times every year, the control of other curing conditions and disease and pest is all identical, used the apple tree that the apple of foliar nutrient of the present invention is not used relatively, growing way is better, and output promotes to some extent, and disease resistance improves simultaneously.
Claims (2)
1. the foliar nutrient produced of a high nutritious fermentation liquor, it is characterized in that, be made up of the raw material of following weight part: trester 15 ~ 20, edible fungi residue 45 ~ 60, EM microbial inoculum 2 ~ 3, saturated limewater 10 ~ 15, urea 7 ~ 10, monoammonium phosphate 6 ~ 10, earthworm soil 15 ~ 25, poultry feather 10 ~ 15, chinaberry fruit 20 ~ 30, vegetable seed slag 50 ~ 60, precipitation agent 8 ~ 10 and appropriate water;
Described sinking agent is made up of the raw material of following weight part: lime powder 15 ~ 20,10% alum liquor 12 ~ 16,10% acetic acid solution 4 ~ 6, primary isoamyl alcohol 0.6 ~ 1.0, polyvinyl alcohol 6 ~ 8, tween 80 1.5 ~ 3, industrial Viscotrol C 2 ~ 4, ethanol 4 ~ 7 and water 50; The mixing of second alcohol and water is poured in reactor by preparation method, then polyvinyl alcohol is added, be heated to 70 ~ 80 DEG C of stirring and dissolving, then add 10% alum liquor, 10% acetic acid solution, primary isoamyl alcohol and lime powder, keep temperature of reaction kettle, adjustment rotating speed 150 ~ 200r/min stirs 20 ~ 35min, finally add tween 80 and industrial Viscotrol C simultaneously, insulated and stirred 30min is emulsion uniformly, obtains residue by funnel suction filtration, pulverizes to obtain pulvis sinking agent after oven dry.
2. the preparation method of foliar nutrient that produces of a kind of high nutritious fermentation liquor according to claim 1, is characterized in that, comprise following step:
(1) mix trester, edible fungi residue, earthworm soil, poultry feather, chinaberry fruit and vegetable seed slag, add the water of 10 ~ 15 times of its weight parts, ball milling 30 ~ 40min, to homogenate, pours in fermentor tank, carries out high-temperature sterilization process;
(2) EM microbial inoculum is added the water dilution of 5 times of its weight parts, be modulated into bacterium liquid, be then seeded in the fermentor tank of (1), adjustment pH5.5 ~ 7, temperature 25 ~ 28 DEG C, passes into sterile air simultaneously and stirs, and ferments 5 ~ 8 days;
(3) after (2) complete, fermented liquid is separately put in container, ultrasonic cell disruption instrument is utilized to carry out microbial disruption, with flowing water, container is lowered the temperature simultaneously, then precipitation agent mixing is added, add saturated limewater again, after stirring 8 ~ 10min, keep fermented liquid inertial motion, leave standstill 20 ~ 25min and carry out precipitation adsorption process voluntarily, supernatant liquor is obtained after completing;
(4) mixing urea, monoammonium phosphate and other remaining component, be added in the supernatant liquor described in (3), stirring at room temperature 45 ~ 60min adsorbs, after completing.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107892617A (en) * | 2017-11-29 | 2018-04-10 | 佛山科学技术学院 | A kind of composite foliage fertilizer and preparation method thereof |
US11076601B2 (en) | 2015-12-23 | 2021-08-03 | Bioboon Limited | Phytosanitary composition |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104177195A (en) * | 2014-09-11 | 2014-12-03 | 标优美生态工程股份有限公司 | Special fertilizer for foliage spraying type flowers and preparation method thereof |
CN104803792A (en) * | 2015-04-28 | 2015-07-29 | 云南省农业科学院热带亚热带经济作物研究所 | Bagasse bio-organic fertilizer and preparation method thereof |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104177195A (en) * | 2014-09-11 | 2014-12-03 | 标优美生态工程股份有限公司 | Special fertilizer for foliage spraying type flowers and preparation method thereof |
CN104803792A (en) * | 2015-04-28 | 2015-07-29 | 云南省农业科学院热带亚热带经济作物研究所 | Bagasse bio-organic fertilizer and preparation method thereof |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11076601B2 (en) | 2015-12-23 | 2021-08-03 | Bioboon Limited | Phytosanitary composition |
CN107892617A (en) * | 2017-11-29 | 2018-04-10 | 佛山科学技术学院 | A kind of composite foliage fertilizer and preparation method thereof |
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