CN109076879A - A kind of selenium-rich gold needle mushroom and its production method - Google Patents

A kind of selenium-rich gold needle mushroom and its production method Download PDF

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Publication number
CN109076879A
CN109076879A CN201811312557.3A CN201811312557A CN109076879A CN 109076879 A CN109076879 A CN 109076879A CN 201811312557 A CN201811312557 A CN 201811312557A CN 109076879 A CN109076879 A CN 109076879A
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selenium
needle mushroom
bottle
rich
raw material
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刘伟历
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Shanghai Pinrou Cultural Development Co Ltd
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Shanghai Pinrou Cultural Development Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost

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  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The present invention relates to a kind of selenium-rich gold needle mushroom and its production method, this method includes: step 1, preparing filler: preparing raw material in proportion, be stirred, then add water under agitation, continues wet-mixing;Raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive, weight ratio 550:145:130:50:880:165:63:500;Step 2, it bottles;Step 3, it sterilizes;Step 4, cooling;Step 5, it is inoculated with;Step 6, cultivate: incubation time is 19~21 days, is divided into first stage and second stage, and it also includes mycelium stimulation and inserted sheet in incubation that the environmental condition of room is given birth in control;Step 7, it harvests and packs.The present invention also provides the selenium-rich gold needle mushrooms produced by this method.The present invention in needle mushroom planting technology by adding selenium raw material and it being made to realize enrichment in needle mushroom, to solve the problems, such as the selenium deficiency in existing nutrition intake.

Description

A kind of selenium-rich gold needle mushroom and its production method
Technical field
The present invention relates to a kind of needle mushroom and its production methods, and in particular, to a kind of selenium-rich gold needle mushroom and its producer Method.
Background technique
The whole world has a countries and regions more than 40 to belong to selenium deficiency area.China is a selenium deficiency big country, according to " the Chinese people Republic's localized disease and environmental factor atlas " it discloses, it is tiltedly worn to the Yunnan-Guizhou Plateau from three provinces in the northeast of China, accounts for Chinese national territorial area There are a low selenium areas in 72% area, wherein 30% is serious selenium deficiency area, the natural foods Se content such as grain is lower.China The big and medium-sized cities such as north, northeast, northwest belong to selenium deficiency area.Some areas of middle 12 provinces and cities of state-two, about 700,000,000 people life In low selenium area.Most area Selenium In Foods content is in 0.02mg/kg hereinafter, southeastern coast is Chinese selenium-enriched area, also only There is 0.10mg/kg, is minimum as defined in the United Nations's health organization.Everyone daily 40 micrograms are still not achieved in Chinese population food Above standard selenium-supply is horizontal, especially selenium deficiency area crowd, it more difficult to achieve the purpose that selenium-supply from food.
Chinese Scientists in succession carry out the Se content of the pedotheque of Chinese 1094 counties and cities' (accounting for about national half) Measurement, measurement result are shown: China is the country of a selenium deficiency.The county for reaching the normal critical value 0.1mg/kg of international publication only has 1/3, i.e. 2/3 area of China belongs to selenium deficiency area.Wherein content≤0.02mg/kg accounts for 29%, for serious selenium deficiency area.
Nutrition expert show that the content standard value of blood selenium is 0.10mg/kg in human body by experiment repeatedly, is lower than this value Selenium deficiency disease will occur.And there is 29% regional selenium content per capita in China in 0.02mg/kg or less, it is determined as extreme selenium deficiency area has 43% regional human body value containing selenium is selenium deficiency area, in other words, there is 72% area in China between 0.03-0.04mg/kg Threat of totally 15 provinces and regions by selenium deficiency.The UN organizations (FAO/WHO/IEA) have formulated answers in human body ordinary meal supply amount There are 400 microgram selenium, and daily selenium intake is only 60 micrograms to Beijing residents per capita.The daily selenium intake per capita of northeast most area Amount only has 10 micrograms, is the district occurred frequently of heart disease, Kaschin-Beck disease.
Most of China area per capita daily selenium intake only between 50-250 microgram.The population of China 2/3 seriously lacks Selenium shows selenium intake wretched insufficiency.
Selenium is classified as 15 kinds daily by " the daily diet nutrient supply amount " of revision Chinese Soclety of Nutrition's in October, 1998 One of diet nutrient proposes that an adult is daily 50-250 microgram to the suitable intake of selenium.Selenium can not in human body Synthesis, so to meet the needs of human body is to selenium, it is necessary to daily Selenium Supplement.By World Health Organization's requirement: in human body diet Daily Minimum requirements amount is 40 microgram selenium, and nutritional supplementation is advisable in 50--250 microgram selenium.
On June 12nd, 2014, Chinese Soclety of Nutrition have formally issued 2013 editions " Chinese residents diet nutrient ginsengs in Shanghai Examine intake (DRIs) ", after 10 years, according to the change of Chinese residents dietary structure and the domestic and international newest scientific research of nutrition educational circles Achievement has made the adjustment of dietary nutrition structure;The day nutrition intake minimum flow of selenium is wherein transferred to 60 micrograms from 50 micrograms.
The content of needle mushroom amino acid is very rich, is higher than general mushroom class, and the content of especially lysine is especially high, relies ammonia Acid has the function of promoting children's intelligence development.Needle mushroom artificial cultivation technique is simultaneously uncomplicated, as long as can control environmental condition, It is easy for obtaining reliable and stable yield.Artificial cultivation needle mushroom technology is combined with selenium-supply, by directly edible Selenium intake wretched insufficiency is solved the problems, such as to a certain extent.
Summary of the invention
Needle mushroom the object of the present invention is to provide a kind of method simply easily realized to produce selenium-rich, in needle mushroom kind Selenium raw material is added in planting technology and it is made to realize enrichment in needle mushroom, so that the selenium deficiency solved in existing nutrition intake is asked Topic.
In order to achieve the above object, the present invention provides a kind of production methods of selenium-rich gold needle mushroom, wherein this method includes Following steps: it step 1, prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, after Continuous wet-mixing;The raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich addition Material;Step 2, it bottles: uniform filling being added in the culture bottle of unified specification, be stirred, then the filler in bottle is beaten Then hole will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary;Step 3, it sterilizes: by what is filled It is put into sterilization stove after autoclave is closed, heat sterilization, data parameters of autoclave such as weight, temperature, pressure etc. can in outside Depending on average every 30min carries out it to detect primary;Step 4, cooling: to open sterilization stove, buffer and steam is discharged, after buffering It comes out of the stove, cooling chamber is put into after culture bottle is taken out, and detect filler temperature in bottle before inoculation;Step 5, it is inoculated with: in each training Needle mushroom, which is uniformly carried out, in feeding bottle connects bacterium;Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, when culture Between be 19~21 days, be divided into first stage and second stage, during the cultivation process the environmental condition of control fertility room, including temperature Degree, humidity, illumination, ventilation etc., and primary shifting library is carried out within every 7 days, it also include mycelium stimulation and inserted sheet in incubation;Step 7, it harvests And packaging.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein corncob, cotton seed hulls, beet in raw material described in step 1 Slag, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive weight ratio be 550:145:130:50:880:165:63:500.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein raw material is stirred using blender in the step 1 It mixes, first progress dry mixing 15min, then adds water while agitating, adding water process is 15min, i.e., stirs while adding water 15min adds water to complete, and carries out wet-mixing 60min again after adding water, makes the water content 67 ± 1.5% of raw material after stirring, pH value 6.2 ~6.8.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein bottled, stirred using bottling machine in the step 2 And punching, every bottle of weight of loading are 880~930g, the mixing time after filler bottling is 4~6.5s, bottling charge level to bottleneck Distance is 1.8~2.0cm.The stirring rod and secondary perforation rod that bottling machine is used to stir and punch need to be cleared up respectively.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein the sterilizing in the step 3 is kept the temperature at 100 DEG C 60min, then in 121 DEG C of heat preservation 70min, then boil in a covered pot over a slow fire and set 20min, the on-off ratio of exhaust valve is 30:10 in sterilization process;After sterilizing The water content of raw material is 64.5~67.5%, and pH value is 5.8~6.2.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein the cooling in the step 4 first turns on heat-insulated buffering Fresh air is flowed into, sterilization stove front door 10cm is then opened, notice draws furnace personnel to open fire door after autoclave immediately, fully opens and goes out Fire door after bacterium pot, buffering row's steam, buffer time 15min close sterilization stove front door after buffering, start to come out of the stove, will train It supports after bottle takes out and is put into cooling chamber, put neat, cooling, filler temperature in detection bottle before being inoculated with, in the range of 15~22 DEG C.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein every bottle of inoculum concentration is 30 ± 3ml in the step 5.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein the culture in the step 6, when the culture of first stage Between be 1~7 day, fertility room space temperature be 14~16 DEG C, between bottle temperature be 14~18 DEG C, natural humidity, CO2Concentration is 2000 ~3000ppm;The incubation time of second stage is 12~20 days, and fertility room space temperature is 12~14 DEG C, and temperature is 14 between bottle ~21 DEG C, humidity is 65~75%, CO2Concentration is 3000~4000ppm;It is carried out in incubation when mycelia reaches bottle 1~2 day Mycelium stimulation, mycelia forbid mycelium stimulation when covering in bottle below 95% area, the control of mycelium stimulation height is the charge level and bottleneck distance after mycelium stimulation For 2.7 ± 0.1cm, keep charge level smooth after mycelium stimulation, and charge level is rinsed well;Bottleneck height 1.5 is grown in needle mushroom thallus again Inserted sheet is carried out when~2.5cm.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein the harvesting in the step 7 is the height in needle mushroom thallus Degree carries out when being 16 ± 1cm.
The present invention also provides the selenium-rich gold needle mushrooms produced by above-mentioned method.
Selenium-rich gold needle mushroom provided by the invention and its production method have the advantage that
Production technology economy of the invention, conveniently, carries out cultivation production using cheap raw material, not by selenium-rich soil Territory restriction, addition selenium element can not only shorten the growth cycle of needle mushroom, and can increase its biomass, while its bacterium The protein and polyoses content of silk and fructification are higher, and a large amount of enrichments of organic selenium may be implemented.Produced needle mushroom is rich in selenium Element, after selenium-enriching cultivation, the presentation of its own active medicinal matter level is increased, and nutritive value enriches and yield height, mouthfeel It is good, it is relatively beneficial to health, is suitble to long-term consumption, is able to solve the selenium deficiency situation of most people.Selenium in selenium-rich gold needle mushroom be with Selenium polysaccharide, selenoprotein and seleno nucleic acid are main existence form, have anti-oxidant, anticancer, antiviral, enhancing animal immunizing power, drop It the effects of blood glucose, reducing blood lipid, can improve the immunity of the human body and resistance, promote the proliferation and antibody and immune ball of lymphocyte The synthesis of albumen, can anticancer, there are also anti-aging functions.
Specific embodiment
A specific embodiment of the invention is further described below.
The production method of selenium-rich gold needle mushroom provided by the invention comprising the steps of: step 1, prepare filler: quasi- in proportion Standby raw material, is then stirred mixing, then adds water under agitation, continues wet-mixing;Raw material includes: corncob, cotton seed hulls, sweet tea Dish slag, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive;Step 2, it bottles: uniform filling is added to the training of unified specification It supports in bottle, is stirred, then the filler in bottle is punched, then the culture bottle equipped with filler is put into autoclave, often Pot is weighed primary after filling;Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, autoclave Data parameters such as weight, temperature, pressure etc. in exterior visual, average every 30min carries out it to detect primary;Step 4, cold But: opening sterilization stove, buffering is discharged steam, comes out of the stove after buffering, cooling chamber is put into after culture bottle is taken out, and before inoculation Detect filler temperature in bottle;Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium;Step 6, it cultivates: will connect Culture bottle after kind is put into fertility room and is cultivated, and incubation time is 19~21 days, is divided into first stage and second stage, is training The environmental condition, including temperature, humidity, illumination, ventilation etc. of control fertility room in the process are supported, and carries out within every 7 days primary shifting library, training It also include mycelium stimulation and inserted sheet during supporting;Step 7, it harvests and packs.
Corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive in the raw material of step 1 Weight ratio be 550:145:130:50:880:165:63:500;Preferably corncob 550kg, cotton seed hulls 145kg, megasse 130kg, conch meal 50kg, rice bran 880kg, wheat bran 165kg, corn flour 63kg, selenium-rich additive 500kg.Selenium-rich additive is Selenium-rich raw material or artificial selenium-rich material, selenium-rich raw material include weed tree sawdust, wheat bran, the stalk etc. of selenium-rich;Artificial selenium-rich material Comprising organic selenium additive or inorganic selenium additive, inorganic selenium additive mainly contains sodium selenite and sodium selenate etc., organic selenium Additive mainly contains Se-enriched yeast, selenide of carragheen (k-selenocarrageenan) selenoprotein etc..
Raw material is stirred using blender in step 1, first progress dry mixing 15min, is then added while agitating Water, adding water process is 15min, i.e., stirs 15min while adding water and water is added to complete, carry out wet-mixing 60min again after adding water, make to stir The water content for mixing rear raw material is 67 ± 1.5%, and pH value is 6.2~6.8.Specific operation process is successively are as follows: and confirmation raw material solves bag, Batch turning starts blender, and dry mixing adds water (stirring), wet-mixing, the discharging of feel qualification.
It bottled, stirred and is punched using bottling machine in step 2, every bottle of weight of loading is 880~930g, filler bottling Mixing time afterwards is 4~6.5s, and the distance of bottling charge level to bottleneck is 1.8~2.0cm.What bottling machine was used to stir and punch Stirring rod and secondary perforation rod need to be cleared up respectively.
Sterilizing in step 3 is then in 121 DEG C of heat preservation 70min, then to boil in a covered pot over a slow fire in 100 DEG C of heat preservation 60min and set 20min, sterilize The on-off ratio of exhaust valve is 30:10 in the process;The water content of raw material is 64.5~67.5% after sterilizing, and pH value is 5.8~6.2.
Cooling in step 4, first turning on heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, immediately Notice draws furnace personnel to open fire door after autoclave, fully opens fire door after autoclave, the row's of buffering steam, buffer time 15min, Sterilization stove front door is closed after buffering, starts to come out of the stove, is put into cooling chamber after culture bottle is taken out, and puts neat, cooling, inoculation Filler temperature in preceding detection bottle, in the range of 15~22 DEG C.Specific operation process is followed successively by confirmation cooler environment, confirmation sterilizing journey Sequence, blow-on door confirm temperature, come out of the stove, and close fire door, then draw each cooling chamber, put neatly, survey material temperature before being inoculated with.
Every bottle of inoculum concentration is 30 ± 3ml in step 5.
Culture in step 6, the incubation time of first stage are 1~7 day, and fertility room space temperature is 14~16 DEG C, bottle Between temperature be 14~18 DEG C, natural humidity, CO2Concentration is 2000~3000ppm;The incubation time of second stage is 12~20 It, fertility room space temperature is 12~14 DEG C, and temperature is 14~21 DEG C between bottle, and humidity is 65~75%, CO2Concentration be 3000~ 4000ppm;Mycelium stimulation is carried out in incubation when mycelia reaches bottle 1~2 day, mycelia is forbidden scratching when covering in bottle below 95% area Bacterium, the control of mycelium stimulation height be that the charge level and bottleneck distance after mycelium stimulation are 2.7 ± 0.1cm, keep charge level smooth after mycelium stimulation, and by charge level It rinses well;Inserted sheet is carried out when needle mushroom thallus grows bottleneck 1.5~2.5cm of height again.Mycelium stimulation is the bacterium filler surface Kind block is dug up, and occurs that fructification simultaneously from media surface, if filler surface is very dry, can be scratched using scraping, i.e., old bacterium Kind of mycoderma and 5~10mm filler is blocking together wipes off, mechanical stimulus and low temperature stimulation of the mycelia by mycelium stimulation can form former base, have Conducive to flower bud fruiting.For acupuncture needle massee fruiting bodies under conditions of complete darkness, cap growth is slow and small, and multiform is faint at misshapen mushroom Scattering light cap can be stimulated to grow, too strong light can then make stem growth be suppressed, and due to acupuncture needle massee fruiting bodies have There is very strong phototropism, the inserted sheet shading since when mushroom body is grown, and retain a degree of scattering light, then control illumination condition It can induce stem to extend to light.
Harvesting in step 7 is carried out when the height of needle mushroom thallus is 16 ± 1cm.
The present invention also provides the selenium-rich gold needle mushrooms produced by this method.
Selenium-rich gold needle mushroom provided by the invention and its production method are further described below with reference to embodiment.
Embodiment 1
A kind of production method of selenium-rich gold needle mushroom comprising the steps of:
Step 1, it prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, after Continuous wet-mixing
Carry out dry mixing 15min first, then add water while agitating, add water process for 15min, i.e.. while adding water Stirring 15min adds water to complete, and carries out wet-mixing 60min again after adding water, makes the water content 67% of raw material after stirring, pH value 6.5.
Preferably, raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich addition Material, weight ratio 550:145:130:50:880:165:63:500;Preferably corncob 550kg, cotton seed hulls 145kg, beet Slag 130kg, conch meal 50kg, rice bran 880kg, wheat bran 165kg, corn flour 63kg, selenium-rich additive 500kg.Selenium-rich additive For selenium-rich raw material, weed tree sawdust, wheat bran, stalk comprising selenium-rich etc..
Step 2, bottle: by uniform filling be added unified specification culture bottle in, be stirred, then to the filler in bottle into Then row punching will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary.
Preferably, it bottled, stirred and is punched using bottling machine, every bottle of weight of loading is 900g, after filler bottling Mixing time is 5s, and the distance of bottling charge level to bottleneck is 1.9cm.Bottling machine is used for the stirring rod that stirs and punch and secondary beats Hole stick needs to be cleared up respectively.
Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, the data parameters of autoclave In exterior visual, average every 30min carries out it to detect primary for such as weight, temperature, pressure.
Preferably, it in 100 DEG C of heat preservation 60min, then in 121 DEG C of heat preservation 70min, then boils in a covered pot over a slow fire and sets 20min, arranged in sterilization process The on-off ratio of air valve is 30:10;The water content of raw material is 66% after sterilizing, pH value 6.
Step 4, cooling: to open sterilization stove, buffering is discharged steam, comes out of the stove after buffering, be put into after culture bottle is taken out Cooling chamber, and filler temperature in bottle is detected before inoculation.
Preferably, first turning on heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, notice draws furnace immediately Personnel open fire door after autoclave, fully open fire door after autoclave, and buffering arranges steam, buffer time 15min, and buffering terminates Sterilization stove front door is closed afterwards, starts to come out of the stove, is put into cooling chamber after culture bottle is taken out, puts neat, cooling, detects bottle before being inoculated with Middle filler temperature is 20 DEG C.
Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium.
Preferably, every bottle of inoculum concentration is 30ml.
Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, incubation time is 20 days, is divided into first Stage and second stage, the environmental condition, including temperature, humidity, illumination, ventilation etc. of room are given birth in control during the cultivation process, and Primary shifting library is carried out within every 7 days, also includes mycelium stimulation and inserted sheet in incubation.
Preferably, the incubation time of first stage is 5 days, and fertility room space temperature is 15 DEG C, and temperature is 16 DEG C between bottle, from Right humidity, CO2Concentration is 2500ppm;The incubation time of second stage is 15 days, and fertility room space temperature is 13 DEG C, warm between bottle Degree is 17 DEG C, humidity 70%, CO2Concentration is 3500ppm;Mycelium stimulation, mycelia are carried out when mycelia reaches bottle 1.5 days in incubation Forbid mycelium stimulation when covering in bottle below 95% area, the control of mycelium stimulation height be that the charge level and bottleneck distance after mycelium stimulation are 2.7cm, is scratched Make charge level smooth after bacterium, and charge level is rinsed well;Inserted sheet is carried out when needle mushroom thallus grows bottleneck height 2cm again.
Step 7, it harvests and packs.
Preferably, it is harvested when the height of needle mushroom thallus is 16cm.
The present embodiment additionally provides the selenium-rich gold needle mushroom produced by this method.
Embodiment 2
A kind of production method of selenium-rich gold needle mushroom comprising the steps of:
Step 1, it prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, after Continuous wet-mixing
Carry out dry mixing 15min first, then add water while agitating, add water process for 15min, i.e.. while adding water Stirring 15min adds water to complete, and carries out wet-mixing 60min again after adding water, makes the water content 65.5% of raw material after stirring, and pH value is 6.2。
Preferably, raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich addition Material, weight ratio 550:145:130:50:880:165:63:500;Preferably corncob 550kg, cotton seed hulls 145kg, beet Slag 130kg, conch meal 50kg, rice bran 880kg, wheat bran 165kg, corn flour 63kg, selenium-rich additive 500kg.Selenium-rich additive For artificial selenium-rich material, preferably inorganic selenium additive, sodium selenite and sodium selenate etc. are mainly contained.
Step 2, bottle: by uniform filling be added unified specification culture bottle in, be stirred, then to the filler in bottle into Then row punching will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary.
Preferably, it bottled, stirred and is punched using bottling machine, every bottle of weight of loading is 880g, after filler bottling Mixing time is 4s, and the distance of bottling charge level to bottleneck is 1.8cm.Bottling machine is used for the stirring rod that stirs and punch and secondary beats Hole stick needs to be cleared up respectively.
Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, the data parameters of autoclave In exterior visual, average every 30min carries out it to detect primary for such as weight, temperature, pressure.
Preferably, it in 100 DEG C of heat preservation 60min, then in 121 DEG C of heat preservation 70min, then boils in a covered pot over a slow fire and sets 20min, arranged in sterilization process The on-off ratio of air valve is 30:10;The water content of raw material is 64.5% after sterilizing, pH value 5.8.
Step 4, cooling: to open sterilization stove, buffering is discharged steam, comes out of the stove after buffering, be put into after culture bottle is taken out Cooling chamber, and filler temperature in bottle is detected before inoculation.
Preferably, first turning on heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, notice draws furnace immediately Personnel open fire door after autoclave, fully open fire door after autoclave, and buffering arranges steam, buffer time 15min, and buffering terminates Sterilization stove front door is closed afterwards, starts to come out of the stove, is put into cooling chamber after culture bottle is taken out, puts neat, cooling, detects bottle before being inoculated with Middle filler temperature is 15 DEG C.
Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium.
Preferably, every bottle of inoculum concentration is 27ml.
Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, incubation time is 19 days, is divided into first Stage and second stage, the environmental condition, including temperature, humidity, illumination, ventilation etc. of room are given birth in control during the cultivation process, and Primary shifting library is carried out within every 7 days, also includes mycelium stimulation and inserted sheet in incubation.
Preferably, the incubation time of first stage is 1 day, and fertility room space temperature is 14 DEG C, and temperature is 14 DEG C between bottle, from Right humidity, CO2Concentration is 2000ppm;The incubation time of second stage is 18 days, and fertility room space temperature is 12 DEG C, warm between bottle Degree is 14 DEG C, humidity 65%, CO2Concentration is 3000ppm;Mycelium stimulation is carried out when mycelia reaches bottle 1 day in incubation, mycelia is long Forbid mycelium stimulation when in full bottle below 95% area, the control of mycelium stimulation height be that the charge level and bottleneck distance after mycelium stimulation are 2.6cm, mycelium stimulation After keep charge level smooth, and charge level is rinsed well;Inserted sheet is carried out when needle mushroom thallus grows bottleneck height 1.5cm again.
Step 7, it harvests and packs.
Preferably, it is harvested when the height of needle mushroom thallus is 15cm.
The present embodiment additionally provides the selenium-rich gold needle mushroom produced by this method.
Embodiment 3
A kind of production method of selenium-rich gold needle mushroom comprising the steps of:
Step 1, it prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, after Continuous wet-mixing
Carry out dry mixing 15min first, then add water while agitating, add water process for 15min, i.e.. while adding water Stirring 15min adds water to complete, and carries out wet-mixing 60min again after adding water, makes the water content 68.5% of raw material after stirring, and pH value is 6.8。
Preferably, raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich addition Material, weight ratio 550:145:130:50:880:165:63:500;Preferably corncob 550kg, cotton seed hulls 145kg, beet Slag 130kg, conch meal 50kg, rice bran 880kg, wheat bran 165kg, corn flour 63kg, selenium-rich additive 500kg.Selenium-rich additive For artificial selenium-rich material, preferably organic selenium additive, Se-enriched yeast, selenide of carragheen (k-selenocarrageenan) selenium egg are mainly contained It is white etc..
Step 2, bottle: by uniform filling be added unified specification culture bottle in, be stirred, then to the filler in bottle into Then row punching will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary.
Preferably, it bottled, stirred and is punched using bottling machine, every bottle of weight of loading is 930g, after filler bottling Mixing time is 6.5s, and the distance of bottling charge level to bottleneck is 2.0cm.Bottling machine is used for the stirring rod that stirs and punch and secondary Perforation rod needs to be cleared up respectively.
Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, the data parameters of autoclave In exterior visual, average every 30min carries out it to detect primary for such as weight, temperature, pressure.
Preferably, it in 100 DEG C of heat preservation 60min, then in 121 DEG C of heat preservation 70min, then boils in a covered pot over a slow fire and sets 20min, arranged in sterilization process The on-off ratio of air valve is 30:10;The water content of raw material is 67.5% after sterilizing, pH value 6.2.
Step 4, cooling: to open sterilization stove, buffering is discharged steam, comes out of the stove after buffering, be put into after culture bottle is taken out Cooling chamber, and filler temperature in bottle is detected before inoculation.
Preferably, first turning on heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, notice draws furnace immediately Personnel open fire door after autoclave, fully open fire door after autoclave, and buffering arranges steam, buffer time 15min, and buffering terminates Sterilization stove front door is closed afterwards, starts to come out of the stove, is put into cooling chamber after culture bottle is taken out, puts neat, cooling, detects bottle before being inoculated with Middle filler temperature is 22 DEG C.
Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium.
Preferably, every bottle of inoculum concentration is 33ml.
Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, incubation time is 21 days, is divided into first Stage and second stage, the environmental condition, including temperature, humidity, illumination, ventilation etc. of room are given birth in control during the cultivation process, and Primary shifting library is carried out within every 7 days, also includes mycelium stimulation and inserted sheet in incubation.
Preferably, the incubation time of first stage is 7 days, and fertility room space temperature is 16 DEG C, and temperature is 18 DEG C between bottle, from Right humidity, CO2Concentration is 3000ppm;The incubation time of second stage is 14 days, and fertility room space temperature is 14 DEG C, warm between bottle Degree is 21 DEG C, humidity 75%, CO2Concentration is 4000ppm;Mycelium stimulation is carried out when mycelia reaches bottle 2 days in incubation, mycelia is long Forbid mycelium stimulation when in full bottle below 95% area, the control of mycelium stimulation height be that the charge level and bottleneck distance after mycelium stimulation are 2.8cm, mycelium stimulation After keep charge level smooth, and charge level is rinsed well;Inserted sheet is carried out when needle mushroom thallus grows bottleneck height 2.5cm again.
Step 7, it harvests and packs.
Preferably, it is harvested when the height of needle mushroom thallus is 17cm.
The present embodiment additionally provides the selenium-rich gold needle mushroom produced by this method.
The selenium-rich gold needle mushroom for taking the embodiment of the present invention to produce, nearly over thousands of object has been randomly choosed in crowd and has been carried out centainly Stage foretastes, and satisfaction reaches 97% or more, and often edible rear selenium deficiency state is improved.
Selenium-rich gold needle mushroom provided by the invention and its production method pass through the selenium element amount added in culture medium for golden mushroom And needle mushroom planting process, selenium and needle mushroom are combined, the needle mushroom of selenium-rich is produced, it is excellent with selenium to have both itself Effectiveness, so as to achieve the effect that it is directly edible can selenium-supply, become the efficient agricultural product that can improve human health.
It is discussed in detail although the contents of the present invention have passed through above preferred embodiment, but it should be appreciated that above-mentioned Description is not considered as limitation of the present invention.After those skilled in the art have read above content, for of the invention A variety of modifications and substitutions all will be apparent.Therefore, protection scope of the present invention should be limited to the appended claims.

Claims (10)

1. a kind of production method of selenium-rich gold needle mushroom, which is characterized in that the method includes the steps of:
Step 1, it prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, continue wet It mixes;
The raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive;
Step 2, it bottles: uniform filling being added in the culture bottle of unified specification, be stirred, then the filler in bottle is beaten Then hole will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary;
Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, average every 30min detection is primary;
Step 4, cooling: to open sterilization stove, buffering is discharged steam, comes out of the stove after buffering, be put into cooling after culture bottle is taken out Room, and filler temperature in bottle is detected before inoculation;
Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium;
Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, incubation time is 19~21 days, is divided into first Stage and second stage, the during the cultivation process environmental condition of control fertility room, and the primary shifting library of progress in every 7 days, incubation In also include mycelium stimulation and inserted sheet;
Step 7, it harvests and packs.
2. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that corn in raw material described in step 1 Core, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive weight ratio be 550:145:130:50: 880:165:63:500;The selenium-rich additive is selenium-rich raw material or artificial selenium-rich material, and artificial selenium-rich material includes organic Selenium additive or inorganic selenium additive.
3. the production method of selenium-rich gold needle mushroom as claimed in claim 2, which is characterized in that use stirring in the step 1 Machine is stirred raw material, first progress dry mixing 15min, then adds water while agitating, and adding water process is 15min, then into Row wet-mixing 60min, makes the water content 67 ± 1.5% of raw material after stirring, and pH value is 6.2~6.8.
4. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that use bottling in the step 2 Machine is bottled, stirred and is punched, and every bottle of weight of loading is 880~930g, and the mixing time after filler bottling is 4~6.5s, The distance of bottling charge level to bottleneck is 1.8~2.0cm.
5. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that the sterilizing in the step 3 is In 100 DEG C of heat preservation 60min, then in 121 DEG C of heat preservation 70min, then boils in a covered pot over a slow fire and set 20min, the on-off ratio of exhaust valve is in sterilization process 30:10;The water content of raw material is 64.5~67.5% after sterilizing, and pH value is 5.8~6.2.
6. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that the cooling in the step 4, it is first First opening heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, opens fire door after autoclave immediately, and buffering row steams Vapour, buffer time 15min close sterilization stove front door after buffering, start to come out of the stove, be put into cooling after culture bottle is taken out Neat, cooling is put in room, filler temperature in detection bottle before being inoculated with, in the range of 15~22 DEG C.
7. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that connect for every bottle in the step 5 Kind amount is 30 ± 3ml.
8. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that the culture in the step 6, the The incubation time in one stage is 1~7 day, and fertility room space temperature is 14~16 DEG C, and temperature is 14~18 DEG C between bottle, naturally wet Degree, CO2Concentration is 2000~3000ppm;The incubation time of second stage is 12~20 days, and fertility room space temperature is 12~14 DEG C, temperature is 14~21 DEG C between bottle, and humidity is 65~75%, CO2Concentration is 3000~4000ppm;In mycelia in incubation Mycelium stimulation is carried out at full bottle 1~2 day, mycelia forbids mycelium stimulation when covering in bottle below 95% area, mycelium stimulation height is with bottleneck distance 2.7 ± 0.1cm keeps charge level smooth after mycelium stimulation, and charge level is rinsed well;Again needle mushroom thallus grow bottleneck height 1.5~ Inserted sheet is carried out when 2.5cm.
9. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that the harvesting in the step 7 is It is carried out when the height of needle mushroom thallus is 16 ± 1cm.
10. a kind of selenium-rich gold needle mushroom that the method by as described in any one of claim 1~9 produces.
CN201811312557.3A 2018-11-03 2018-11-03 A kind of selenium-rich gold needle mushroom and its production method Pending CN109076879A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115413531A (en) * 2022-10-09 2022-12-02 福建万辰生物科技股份有限公司 Culture material for culturing needle mushrooms and needle mushroom culturing method

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101743843A (en) * 2008-12-18 2010-06-23 上海丰科生物科技股份有限公司 Method for cultivating brown crab taste mushroom
CN104557261A (en) * 2014-12-13 2015-04-29 绿雅(江苏)食用菌有限公司 Pleurotus eryngii culture medium and industrial culture method
CN105557297A (en) * 2015-08-31 2016-05-11 福建省裕兴农业科技有限公司 Basic process flow for industrialized cultivation of flammulina velutipes
CN106962015A (en) * 2016-01-14 2017-07-21 东莞市永俊生物科技股份有限公司 A kind of culture process of asparagus
CN107056387A (en) * 2017-05-20 2017-08-18 天津市宝坻区和泰丰食用菌有限公司 A kind of selenium-rich gold needle mushroom implantation methods
CN108293592A (en) * 2017-10-17 2018-07-20 辽宁天赢生物科技股份有限公司 A method of cultivating needle mushroom using sorghum flour mixture

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101743843A (en) * 2008-12-18 2010-06-23 上海丰科生物科技股份有限公司 Method for cultivating brown crab taste mushroom
CN104557261A (en) * 2014-12-13 2015-04-29 绿雅(江苏)食用菌有限公司 Pleurotus eryngii culture medium and industrial culture method
CN105557297A (en) * 2015-08-31 2016-05-11 福建省裕兴农业科技有限公司 Basic process flow for industrialized cultivation of flammulina velutipes
CN106962015A (en) * 2016-01-14 2017-07-21 东莞市永俊生物科技股份有限公司 A kind of culture process of asparagus
CN107056387A (en) * 2017-05-20 2017-08-18 天津市宝坻区和泰丰食用菌有限公司 A kind of selenium-rich gold needle mushroom implantation methods
CN108293592A (en) * 2017-10-17 2018-07-20 辽宁天赢生物科技股份有限公司 A method of cultivating needle mushroom using sorghum flour mixture

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
周会明: "《食用菌栽培技术》", 31 May 2017, 中国农业大学出版社 *
黄良水: "《现代食用菌生产新技术》", 30 September 2011, 浙江科学技术出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115413531A (en) * 2022-10-09 2022-12-02 福建万辰生物科技股份有限公司 Culture material for culturing needle mushrooms and needle mushroom culturing method

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Application publication date: 20181225