CN109076879A - A kind of selenium-rich gold needle mushroom and its production method - Google Patents
A kind of selenium-rich gold needle mushroom and its production method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention relates to a kind of selenium-rich gold needle mushroom and its production method, this method includes: step 1, preparing filler: preparing raw material in proportion, be stirred, then add water under agitation, continues wet-mixing；Raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive, weight ratio 550:145:130:50:880:165:63:500；Step 2, it bottles；Step 3, it sterilizes；Step 4, cooling；Step 5, it is inoculated with；Step 6, cultivate: incubation time is 19~21 days, is divided into first stage and second stage, and it also includes mycelium stimulation and inserted sheet in incubation that the environmental condition of room is given birth in control；Step 7, it harvests and packs.The present invention also provides the selenium-rich gold needle mushrooms produced by this method.The present invention in needle mushroom planting technology by adding selenium raw material and it being made to realize enrichment in needle mushroom, to solve the problems, such as the selenium deficiency in existing nutrition intake.
Description
Technical field
The present invention relates to a kind of needle mushroom and its production methods, and in particular, to a kind of selenium-rich gold needle mushroom and its producer
Method.
Background technique
The whole world has a countries and regions more than 40 to belong to selenium deficiency area.China is a selenium deficiency big country, according to " the Chinese people
Republic's localized disease and environmental factor atlas " it discloses, it is tiltedly worn to the Yunnan-Guizhou Plateau from three provinces in the northeast of China, accounts for Chinese national territorial area
There are a low selenium areas in 72% area, wherein 30% is serious selenium deficiency area, the natural foods Se content such as grain is lower.China
The big and medium-sized cities such as north, northeast, northwest belong to selenium deficiency area.Some areas of middle 12 provinces and cities of state-two, about 700,000,000 people life
In low selenium area.Most area Selenium In Foods content is in 0.02mg/kg hereinafter, southeastern coast is Chinese selenium-enriched area, also only
There is 0.10mg/kg, is minimum as defined in the United Nations's health organization.Everyone daily 40 micrograms are still not achieved in Chinese population food
Above standard selenium-supply is horizontal, especially selenium deficiency area crowd, it more difficult to achieve the purpose that selenium-supply from food.
Chinese Scientists in succession carry out the Se content of the pedotheque of Chinese 1094 counties and cities' (accounting for about national half)
Measurement, measurement result are shown: China is the country of a selenium deficiency.The county for reaching the normal critical value 0.1mg/kg of international publication only has
1/3, i.e. 2/3 area of China belongs to selenium deficiency area.Wherein content≤0.02mg/kg accounts for 29%, for serious selenium deficiency area.
Nutrition expert show that the content standard value of blood selenium is 0.10mg/kg in human body by experiment repeatedly, is lower than this value
Selenium deficiency disease will occur.And there is 29% regional selenium content per capita in China in 0.02mg/kg or less, it is determined as extreme selenium deficiency area has
43% regional human body value containing selenium is selenium deficiency area, in other words, there is 72% area in China between 0.03-0.04mg/kg
Threat of totally 15 provinces and regions by selenium deficiency.The UN organizations (FAO/WHO/IEA) have formulated answers in human body ordinary meal supply amount
There are 400 microgram selenium, and daily selenium intake is only 60 micrograms to Beijing residents per capita.The daily selenium intake per capita of northeast most area
Amount only has 10 micrograms, is the district occurred frequently of heart disease, Kaschin-Beck disease.
Most of China area per capita daily selenium intake only between 50-250 microgram.The population of China 2/3 seriously lacks
Selenium shows selenium intake wretched insufficiency.
Selenium is classified as 15 kinds daily by " the daily diet nutrient supply amount " of revision Chinese Soclety of Nutrition's in October, 1998
One of diet nutrient proposes that an adult is daily 50-250 microgram to the suitable intake of selenium.Selenium can not in human body
Synthesis, so to meet the needs of human body is to selenium, it is necessary to daily Selenium Supplement.By World Health Organization's requirement: in human body diet
Daily Minimum requirements amount is 40 microgram selenium, and nutritional supplementation is advisable in 50--250 microgram selenium.
On June 12nd, 2014, Chinese Soclety of Nutrition have formally issued 2013 editions " Chinese residents diet nutrient ginsengs in Shanghai
Examine intake (DRIs) ", after 10 years, according to the change of Chinese residents dietary structure and the domestic and international newest scientific research of nutrition educational circles
Achievement has made the adjustment of dietary nutrition structure；The day nutrition intake minimum flow of selenium is wherein transferred to 60 micrograms from 50 micrograms.
The content of needle mushroom amino acid is very rich, is higher than general mushroom class, and the content of especially lysine is especially high, relies ammonia
Acid has the function of promoting children's intelligence development.Needle mushroom artificial cultivation technique is simultaneously uncomplicated, as long as can control environmental condition,
It is easy for obtaining reliable and stable yield.Artificial cultivation needle mushroom technology is combined with selenium-supply, by directly edible
Selenium intake wretched insufficiency is solved the problems, such as to a certain extent.
Summary of the invention
Needle mushroom the object of the present invention is to provide a kind of method simply easily realized to produce selenium-rich, in needle mushroom kind
Selenium raw material is added in planting technology and it is made to realize enrichment in needle mushroom, so that the selenium deficiency solved in existing nutrition intake is asked
Topic.
In order to achieve the above object, the present invention provides a kind of production methods of selenium-rich gold needle mushroom, wherein this method includes
Following steps: it step 1, prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, after
Continuous wet-mixing；The raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich addition
Material；Step 2, it bottles: uniform filling being added in the culture bottle of unified specification, be stirred, then the filler in bottle is beaten
Then hole will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary；Step 3, it sterilizes: by what is filled
It is put into sterilization stove after autoclave is closed, heat sterilization, data parameters of autoclave such as weight, temperature, pressure etc. can in outside
Depending on average every 30min carries out it to detect primary；Step 4, cooling: to open sterilization stove, buffer and steam is discharged, after buffering
It comes out of the stove, cooling chamber is put into after culture bottle is taken out, and detect filler temperature in bottle before inoculation；Step 5, it is inoculated with: in each training
Needle mushroom, which is uniformly carried out, in feeding bottle connects bacterium；Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, when culture
Between be 19~21 days, be divided into first stage and second stage, during the cultivation process the environmental condition of control fertility room, including temperature
Degree, humidity, illumination, ventilation etc., and primary shifting library is carried out within every 7 days, it also include mycelium stimulation and inserted sheet in incubation；Step 7, it harvests
And packaging.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein corncob, cotton seed hulls, beet in raw material described in step 1
Slag, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive weight ratio be 550:145:130:50:880:165:63:500.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein raw material is stirred using blender in the step 1
It mixes, first progress dry mixing 15min, then adds water while agitating, adding water process is 15min, i.e., stirs while adding water
15min adds water to complete, and carries out wet-mixing 60min again after adding water, makes the water content 67 ± 1.5% of raw material after stirring, pH value 6.2
~6.8.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein bottled, stirred using bottling machine in the step 2
And punching, every bottle of weight of loading are 880~930g, the mixing time after filler bottling is 4~6.5s, bottling charge level to bottleneck
Distance is 1.8~2.0cm.The stirring rod and secondary perforation rod that bottling machine is used to stir and punch need to be cleared up respectively.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein the sterilizing in the step 3 is kept the temperature at 100 DEG C
60min, then in 121 DEG C of heat preservation 70min, then boil in a covered pot over a slow fire and set 20min, the on-off ratio of exhaust valve is 30:10 in sterilization process；After sterilizing
The water content of raw material is 64.5~67.5%, and pH value is 5.8~6.2.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein the cooling in the step 4 first turns on heat-insulated buffering
Fresh air is flowed into, sterilization stove front door 10cm is then opened, notice draws furnace personnel to open fire door after autoclave immediately, fully opens and goes out
Fire door after bacterium pot, buffering row's steam, buffer time 15min close sterilization stove front door after buffering, start to come out of the stove, will train
It supports after bottle takes out and is put into cooling chamber, put neat, cooling, filler temperature in detection bottle before being inoculated with, in the range of 15~22 DEG C.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein every bottle of inoculum concentration is 30 ± 3ml in the step 5.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein the culture in the step 6, when the culture of first stage
Between be 1~7 day, fertility room space temperature be 14~16 DEG C, between bottle temperature be 14~18 DEG C, natural humidity, CO2Concentration is 2000
~3000ppm；The incubation time of second stage is 12~20 days, and fertility room space temperature is 12~14 DEG C, and temperature is 14 between bottle
~21 DEG C, humidity is 65~75%, CO2Concentration is 3000~4000ppm；It is carried out in incubation when mycelia reaches bottle 1~2 day
Mycelium stimulation, mycelia forbid mycelium stimulation when covering in bottle below 95% area, the control of mycelium stimulation height is the charge level and bottleneck distance after mycelium stimulation
For 2.7 ± 0.1cm, keep charge level smooth after mycelium stimulation, and charge level is rinsed well；Bottleneck height 1.5 is grown in needle mushroom thallus again
Inserted sheet is carried out when~2.5cm.
The production method of above-mentioned selenium-rich gold needle mushroom, wherein the harvesting in the step 7 is the height in needle mushroom thallus
Degree carries out when being 16 ± 1cm.
The present invention also provides the selenium-rich gold needle mushrooms produced by above-mentioned method.
Selenium-rich gold needle mushroom provided by the invention and its production method have the advantage that
Production technology economy of the invention, conveniently, carries out cultivation production using cheap raw material, not by selenium-rich soil
Territory restriction, addition selenium element can not only shorten the growth cycle of needle mushroom, and can increase its biomass, while its bacterium
The protein and polyoses content of silk and fructification are higher, and a large amount of enrichments of organic selenium may be implemented.Produced needle mushroom is rich in selenium
Element, after selenium-enriching cultivation, the presentation of its own active medicinal matter level is increased, and nutritive value enriches and yield height, mouthfeel
It is good, it is relatively beneficial to health, is suitble to long-term consumption, is able to solve the selenium deficiency situation of most people.Selenium in selenium-rich gold needle mushroom be with
Selenium polysaccharide, selenoprotein and seleno nucleic acid are main existence form, have anti-oxidant, anticancer, antiviral, enhancing animal immunizing power, drop
It the effects of blood glucose, reducing blood lipid, can improve the immunity of the human body and resistance, promote the proliferation and antibody and immune ball of lymphocyte
The synthesis of albumen, can anticancer, there are also anti-aging functions.
Specific embodiment
A specific embodiment of the invention is further described below.
The production method of selenium-rich gold needle mushroom provided by the invention comprising the steps of: step 1, prepare filler: quasi- in proportion
Standby raw material, is then stirred mixing, then adds water under agitation, continues wet-mixing；Raw material includes: corncob, cotton seed hulls, sweet tea
Dish slag, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive；Step 2, it bottles: uniform filling is added to the training of unified specification
It supports in bottle, is stirred, then the filler in bottle is punched, then the culture bottle equipped with filler is put into autoclave, often
Pot is weighed primary after filling；Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, autoclave
Data parameters such as weight, temperature, pressure etc. in exterior visual, average every 30min carries out it to detect primary；Step 4, cold
But: opening sterilization stove, buffering is discharged steam, comes out of the stove after buffering, cooling chamber is put into after culture bottle is taken out, and before inoculation
Detect filler temperature in bottle；Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium；Step 6, it cultivates: will connect
Culture bottle after kind is put into fertility room and is cultivated, and incubation time is 19~21 days, is divided into first stage and second stage, is training
The environmental condition, including temperature, humidity, illumination, ventilation etc. of control fertility room in the process are supported, and carries out within every 7 days primary shifting library, training
It also include mycelium stimulation and inserted sheet during supporting；Step 7, it harvests and packs.
Corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive in the raw material of step 1
Weight ratio be 550:145:130:50:880:165:63:500；Preferably corncob 550kg, cotton seed hulls 145kg, megasse
130kg, conch meal 50kg, rice bran 880kg, wheat bran 165kg, corn flour 63kg, selenium-rich additive 500kg.Selenium-rich additive is
Selenium-rich raw material or artificial selenium-rich material, selenium-rich raw material include weed tree sawdust, wheat bran, the stalk etc. of selenium-rich；Artificial selenium-rich material
Comprising organic selenium additive or inorganic selenium additive, inorganic selenium additive mainly contains sodium selenite and sodium selenate etc., organic selenium
Additive mainly contains Se-enriched yeast, selenide of carragheen (k-selenocarrageenan) selenoprotein etc..
Raw material is stirred using blender in step 1, first progress dry mixing 15min, is then added while agitating
Water, adding water process is 15min, i.e., stirs 15min while adding water and water is added to complete, carry out wet-mixing 60min again after adding water, make to stir
The water content for mixing rear raw material is 67 ± 1.5%, and pH value is 6.2~6.8.Specific operation process is successively are as follows: and confirmation raw material solves bag,
Batch turning starts blender, and dry mixing adds water (stirring), wet-mixing, the discharging of feel qualification.
It bottled, stirred and is punched using bottling machine in step 2, every bottle of weight of loading is 880~930g, filler bottling
Mixing time afterwards is 4~6.5s, and the distance of bottling charge level to bottleneck is 1.8~2.0cm.What bottling machine was used to stir and punch
Stirring rod and secondary perforation rod need to be cleared up respectively.
Sterilizing in step 3 is then in 121 DEG C of heat preservation 70min, then to boil in a covered pot over a slow fire in 100 DEG C of heat preservation 60min and set 20min, sterilize
The on-off ratio of exhaust valve is 30:10 in the process；The water content of raw material is 64.5~67.5% after sterilizing, and pH value is 5.8~6.2.
Cooling in step 4, first turning on heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, immediately
Notice draws furnace personnel to open fire door after autoclave, fully opens fire door after autoclave, the row's of buffering steam, buffer time 15min,
Sterilization stove front door is closed after buffering, starts to come out of the stove, is put into cooling chamber after culture bottle is taken out, and puts neat, cooling, inoculation
Filler temperature in preceding detection bottle, in the range of 15~22 DEG C.Specific operation process is followed successively by confirmation cooler environment, confirmation sterilizing journey
Sequence, blow-on door confirm temperature, come out of the stove, and close fire door, then draw each cooling chamber, put neatly, survey material temperature before being inoculated with.
Every bottle of inoculum concentration is 30 ± 3ml in step 5.
Culture in step 6, the incubation time of first stage are 1~7 day, and fertility room space temperature is 14~16 DEG C, bottle
Between temperature be 14~18 DEG C, natural humidity, CO2Concentration is 2000~3000ppm；The incubation time of second stage is 12~20
It, fertility room space temperature is 12~14 DEG C, and temperature is 14~21 DEG C between bottle, and humidity is 65~75%, CO2Concentration be 3000~
4000ppm；Mycelium stimulation is carried out in incubation when mycelia reaches bottle 1~2 day, mycelia is forbidden scratching when covering in bottle below 95% area
Bacterium, the control of mycelium stimulation height be that the charge level and bottleneck distance after mycelium stimulation are 2.7 ± 0.1cm, keep charge level smooth after mycelium stimulation, and by charge level
It rinses well；Inserted sheet is carried out when needle mushroom thallus grows bottleneck 1.5~2.5cm of height again.Mycelium stimulation is the bacterium filler surface
Kind block is dug up, and occurs that fructification simultaneously from media surface, if filler surface is very dry, can be scratched using scraping, i.e., old bacterium
Kind of mycoderma and 5~10mm filler is blocking together wipes off, mechanical stimulus and low temperature stimulation of the mycelia by mycelium stimulation can form former base, have
Conducive to flower bud fruiting.For acupuncture needle massee fruiting bodies under conditions of complete darkness, cap growth is slow and small, and multiform is faint at misshapen mushroom
Scattering light cap can be stimulated to grow, too strong light can then make stem growth be suppressed, and due to acupuncture needle massee fruiting bodies have
There is very strong phototropism, the inserted sheet shading since when mushroom body is grown, and retain a degree of scattering light, then control illumination condition
It can induce stem to extend to light.
Harvesting in step 7 is carried out when the height of needle mushroom thallus is 16 ± 1cm.
The present invention also provides the selenium-rich gold needle mushrooms produced by this method.
Selenium-rich gold needle mushroom provided by the invention and its production method are further described below with reference to embodiment.
Embodiment 1
A kind of production method of selenium-rich gold needle mushroom comprising the steps of:
Step 1, it prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, after
Continuous wet-mixing
Carry out dry mixing 15min first, then add water while agitating, add water process for 15min, i.e.. while adding water
Stirring 15min adds water to complete, and carries out wet-mixing 60min again after adding water, makes the water content 67% of raw material after stirring, pH value 6.5.
Preferably, raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich addition
Material, weight ratio 550:145:130:50:880:165:63:500；Preferably corncob 550kg, cotton seed hulls 145kg, beet
Slag 130kg, conch meal 50kg, rice bran 880kg, wheat bran 165kg, corn flour 63kg, selenium-rich additive 500kg.Selenium-rich additive
For selenium-rich raw material, weed tree sawdust, wheat bran, stalk comprising selenium-rich etc..
Step 2, bottle: by uniform filling be added unified specification culture bottle in, be stirred, then to the filler in bottle into
Then row punching will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary.
Preferably, it bottled, stirred and is punched using bottling machine, every bottle of weight of loading is 900g, after filler bottling
Mixing time is 5s, and the distance of bottling charge level to bottleneck is 1.9cm.Bottling machine is used for the stirring rod that stirs and punch and secondary beats
Hole stick needs to be cleared up respectively.
Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, the data parameters of autoclave
In exterior visual, average every 30min carries out it to detect primary for such as weight, temperature, pressure.
Preferably, it in 100 DEG C of heat preservation 60min, then in 121 DEG C of heat preservation 70min, then boils in a covered pot over a slow fire and sets 20min, arranged in sterilization process
The on-off ratio of air valve is 30:10；The water content of raw material is 66% after sterilizing, pH value 6.
Step 4, cooling: to open sterilization stove, buffering is discharged steam, comes out of the stove after buffering, be put into after culture bottle is taken out
Cooling chamber, and filler temperature in bottle is detected before inoculation.
Preferably, first turning on heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, notice draws furnace immediately
Personnel open fire door after autoclave, fully open fire door after autoclave, and buffering arranges steam, buffer time 15min, and buffering terminates
Sterilization stove front door is closed afterwards, starts to come out of the stove, is put into cooling chamber after culture bottle is taken out, puts neat, cooling, detects bottle before being inoculated with
Middle filler temperature is 20 DEG C.
Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium.
Preferably, every bottle of inoculum concentration is 30ml.
Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, incubation time is 20 days, is divided into first
Stage and second stage, the environmental condition, including temperature, humidity, illumination, ventilation etc. of room are given birth in control during the cultivation process, and
Primary shifting library is carried out within every 7 days, also includes mycelium stimulation and inserted sheet in incubation.
Preferably, the incubation time of first stage is 5 days, and fertility room space temperature is 15 DEG C, and temperature is 16 DEG C between bottle, from
Right humidity, CO2Concentration is 2500ppm；The incubation time of second stage is 15 days, and fertility room space temperature is 13 DEG C, warm between bottle
Degree is 17 DEG C, humidity 70%, CO2Concentration is 3500ppm；Mycelium stimulation, mycelia are carried out when mycelia reaches bottle 1.5 days in incubation
Forbid mycelium stimulation when covering in bottle below 95% area, the control of mycelium stimulation height be that the charge level and bottleneck distance after mycelium stimulation are 2.7cm, is scratched
Make charge level smooth after bacterium, and charge level is rinsed well；Inserted sheet is carried out when needle mushroom thallus grows bottleneck height 2cm again.
Step 7, it harvests and packs.
Preferably, it is harvested when the height of needle mushroom thallus is 16cm.
The present embodiment additionally provides the selenium-rich gold needle mushroom produced by this method.
Embodiment 2
A kind of production method of selenium-rich gold needle mushroom comprising the steps of:
Step 1, it prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, after
Continuous wet-mixing
Carry out dry mixing 15min first, then add water while agitating, add water process for 15min, i.e.. while adding water
Stirring 15min adds water to complete, and carries out wet-mixing 60min again after adding water, makes the water content 65.5% of raw material after stirring, and pH value is
6.2。
Preferably, raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich addition
Material, weight ratio 550:145:130:50:880:165:63:500；Preferably corncob 550kg, cotton seed hulls 145kg, beet
Slag 130kg, conch meal 50kg, rice bran 880kg, wheat bran 165kg, corn flour 63kg, selenium-rich additive 500kg.Selenium-rich additive
For artificial selenium-rich material, preferably inorganic selenium additive, sodium selenite and sodium selenate etc. are mainly contained.
Step 2, bottle: by uniform filling be added unified specification culture bottle in, be stirred, then to the filler in bottle into
Then row punching will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary.
Preferably, it bottled, stirred and is punched using bottling machine, every bottle of weight of loading is 880g, after filler bottling
Mixing time is 4s, and the distance of bottling charge level to bottleneck is 1.8cm.Bottling machine is used for the stirring rod that stirs and punch and secondary beats
Hole stick needs to be cleared up respectively.
Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, the data parameters of autoclave
In exterior visual, average every 30min carries out it to detect primary for such as weight, temperature, pressure.
Preferably, it in 100 DEG C of heat preservation 60min, then in 121 DEG C of heat preservation 70min, then boils in a covered pot over a slow fire and sets 20min, arranged in sterilization process
The on-off ratio of air valve is 30:10；The water content of raw material is 64.5% after sterilizing, pH value 5.8.
Step 4, cooling: to open sterilization stove, buffering is discharged steam, comes out of the stove after buffering, be put into after culture bottle is taken out
Cooling chamber, and filler temperature in bottle is detected before inoculation.
Preferably, first turning on heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, notice draws furnace immediately
Personnel open fire door after autoclave, fully open fire door after autoclave, and buffering arranges steam, buffer time 15min, and buffering terminates
Sterilization stove front door is closed afterwards, starts to come out of the stove, is put into cooling chamber after culture bottle is taken out, puts neat, cooling, detects bottle before being inoculated with
Middle filler temperature is 15 DEG C.
Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium.
Preferably, every bottle of inoculum concentration is 27ml.
Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, incubation time is 19 days, is divided into first
Stage and second stage, the environmental condition, including temperature, humidity, illumination, ventilation etc. of room are given birth in control during the cultivation process, and
Primary shifting library is carried out within every 7 days, also includes mycelium stimulation and inserted sheet in incubation.
Preferably, the incubation time of first stage is 1 day, and fertility room space temperature is 14 DEG C, and temperature is 14 DEG C between bottle, from
Right humidity, CO2Concentration is 2000ppm；The incubation time of second stage is 18 days, and fertility room space temperature is 12 DEG C, warm between bottle
Degree is 14 DEG C, humidity 65%, CO2Concentration is 3000ppm；Mycelium stimulation is carried out when mycelia reaches bottle 1 day in incubation, mycelia is long
Forbid mycelium stimulation when in full bottle below 95% area, the control of mycelium stimulation height be that the charge level and bottleneck distance after mycelium stimulation are 2.6cm, mycelium stimulation
After keep charge level smooth, and charge level is rinsed well；Inserted sheet is carried out when needle mushroom thallus grows bottleneck height 1.5cm again.
Step 7, it harvests and packs.
Preferably, it is harvested when the height of needle mushroom thallus is 15cm.
The present embodiment additionally provides the selenium-rich gold needle mushroom produced by this method.
Embodiment 3
A kind of production method of selenium-rich gold needle mushroom comprising the steps of:
Step 1, it prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, after
Continuous wet-mixing
Carry out dry mixing 15min first, then add water while agitating, add water process for 15min, i.e.. while adding water
Stirring 15min adds water to complete, and carries out wet-mixing 60min again after adding water, makes the water content 68.5% of raw material after stirring, and pH value is
6.8。
Preferably, raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich addition
Material, weight ratio 550:145:130:50:880:165:63:500；Preferably corncob 550kg, cotton seed hulls 145kg, beet
Slag 130kg, conch meal 50kg, rice bran 880kg, wheat bran 165kg, corn flour 63kg, selenium-rich additive 500kg.Selenium-rich additive
For artificial selenium-rich material, preferably organic selenium additive, Se-enriched yeast, selenide of carragheen (k-selenocarrageenan) selenium egg are mainly contained
It is white etc..
Step 2, bottle: by uniform filling be added unified specification culture bottle in, be stirred, then to the filler in bottle into
Then row punching will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary.
Preferably, it bottled, stirred and is punched using bottling machine, every bottle of weight of loading is 930g, after filler bottling
Mixing time is 6.5s, and the distance of bottling charge level to bottleneck is 2.0cm.Bottling machine is used for the stirring rod that stirs and punch and secondary
Perforation rod needs to be cleared up respectively.
Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, the data parameters of autoclave
In exterior visual, average every 30min carries out it to detect primary for such as weight, temperature, pressure.
Preferably, it in 100 DEG C of heat preservation 60min, then in 121 DEG C of heat preservation 70min, then boils in a covered pot over a slow fire and sets 20min, arranged in sterilization process
The on-off ratio of air valve is 30:10；The water content of raw material is 67.5% after sterilizing, pH value 6.2.
Step 4, cooling: to open sterilization stove, buffering is discharged steam, comes out of the stove after buffering, be put into after culture bottle is taken out
Cooling chamber, and filler temperature in bottle is detected before inoculation.
Preferably, first turning on heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, notice draws furnace immediately
Personnel open fire door after autoclave, fully open fire door after autoclave, and buffering arranges steam, buffer time 15min, and buffering terminates
Sterilization stove front door is closed afterwards, starts to come out of the stove, is put into cooling chamber after culture bottle is taken out, puts neat, cooling, detects bottle before being inoculated with
Middle filler temperature is 22 DEG C.
Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium.
Preferably, every bottle of inoculum concentration is 33ml.
Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, incubation time is 21 days, is divided into first
Stage and second stage, the environmental condition, including temperature, humidity, illumination, ventilation etc. of room are given birth in control during the cultivation process, and
Primary shifting library is carried out within every 7 days, also includes mycelium stimulation and inserted sheet in incubation.
Preferably, the incubation time of first stage is 7 days, and fertility room space temperature is 16 DEG C, and temperature is 18 DEG C between bottle, from
Right humidity, CO2Concentration is 3000ppm；The incubation time of second stage is 14 days, and fertility room space temperature is 14 DEG C, warm between bottle
Degree is 21 DEG C, humidity 75%, CO2Concentration is 4000ppm；Mycelium stimulation is carried out when mycelia reaches bottle 2 days in incubation, mycelia is long
Forbid mycelium stimulation when in full bottle below 95% area, the control of mycelium stimulation height be that the charge level and bottleneck distance after mycelium stimulation are 2.8cm, mycelium stimulation
After keep charge level smooth, and charge level is rinsed well；Inserted sheet is carried out when needle mushroom thallus grows bottleneck height 2.5cm again.
Step 7, it harvests and packs.
Preferably, it is harvested when the height of needle mushroom thallus is 17cm.
The present embodiment additionally provides the selenium-rich gold needle mushroom produced by this method.
The selenium-rich gold needle mushroom for taking the embodiment of the present invention to produce, nearly over thousands of object has been randomly choosed in crowd and has been carried out centainly
Stage foretastes, and satisfaction reaches 97% or more, and often edible rear selenium deficiency state is improved.
Selenium-rich gold needle mushroom provided by the invention and its production method pass through the selenium element amount added in culture medium for golden mushroom
And needle mushroom planting process, selenium and needle mushroom are combined, the needle mushroom of selenium-rich is produced, it is excellent with selenium to have both itself
Effectiveness, so as to achieve the effect that it is directly edible can selenium-supply, become the efficient agricultural product that can improve human health.
It is discussed in detail although the contents of the present invention have passed through above preferred embodiment, but it should be appreciated that above-mentioned
Description is not considered as limitation of the present invention.After those skilled in the art have read above content, for of the invention
A variety of modifications and substitutions all will be apparent.Therefore, protection scope of the present invention should be limited to the appended claims.
Claims (10)
1. a kind of production method of selenium-rich gold needle mushroom, which is characterized in that the method includes the steps of:
Step 1, it prepares filler: preparing raw material in proportion, be then stirred mixing, then add water under agitation, continue wet
It mixes；
The raw material includes: corncob, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive；
Step 2, it bottles: uniform filling being added in the culture bottle of unified specification, be stirred, then the filler in bottle is beaten
Then hole will be put into autoclave equipped with the culture bottle of filler, every pot fill after weigh it is primary；
Step 3, sterilize: by the autoclave filled it is closed after be put into sterilization stove, heat sterilization, average every 30min detection is primary；
Step 4, cooling: to open sterilization stove, buffering is discharged steam, comes out of the stove after buffering, be put into cooling after culture bottle is taken out
Room, and filler temperature in bottle is detected before inoculation；
Step 5, it is inoculated with: uniformly carrying out needle mushroom in each culture bottle and connect bacterium；
Step 6, it cultivates: the culture bottle after inoculation being put into fertility room and is cultivated, incubation time is 19~21 days, is divided into first
Stage and second stage, the during the cultivation process environmental condition of control fertility room, and the primary shifting library of progress in every 7 days, incubation
In also include mycelium stimulation and inserted sheet；
Step 7, it harvests and packs.
2. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that corn in raw material described in step 1
Core, cotton seed hulls, megasse, conch meal, rice bran, wheat bran, corn flour, selenium-rich additive weight ratio be 550:145:130:50:
880:165:63:500；The selenium-rich additive is selenium-rich raw material or artificial selenium-rich material, and artificial selenium-rich material includes organic
Selenium additive or inorganic selenium additive.
3. the production method of selenium-rich gold needle mushroom as claimed in claim 2, which is characterized in that use stirring in the step 1
Machine is stirred raw material, first progress dry mixing 15min, then adds water while agitating, and adding water process is 15min, then into
Row wet-mixing 60min, makes the water content 67 ± 1.5% of raw material after stirring, and pH value is 6.2~6.8.
4. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that use bottling in the step 2
Machine is bottled, stirred and is punched, and every bottle of weight of loading is 880~930g, and the mixing time after filler bottling is 4~6.5s,
The distance of bottling charge level to bottleneck is 1.8~2.0cm.
5. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that the sterilizing in the step 3 is
In 100 DEG C of heat preservation 60min, then in 121 DEG C of heat preservation 70min, then boils in a covered pot over a slow fire and set 20min, the on-off ratio of exhaust valve is in sterilization process
30:10；The water content of raw material is 64.5~67.5% after sterilizing, and pH value is 5.8~6.2.
6. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that the cooling in the step 4, it is first
First opening heat-insulated buffering flows into fresh air, then opens sterilization stove front door 10cm, opens fire door after autoclave immediately, and buffering row steams
Vapour, buffer time 15min close sterilization stove front door after buffering, start to come out of the stove, be put into cooling after culture bottle is taken out
Neat, cooling is put in room, filler temperature in detection bottle before being inoculated with, in the range of 15~22 DEG C.
7. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that connect for every bottle in the step 5
Kind amount is 30 ± 3ml.
8. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that the culture in the step 6, the
The incubation time in one stage is 1~7 day, and fertility room space temperature is 14~16 DEG C, and temperature is 14~18 DEG C between bottle, naturally wet
Degree, CO2Concentration is 2000~3000ppm；The incubation time of second stage is 12~20 days, and fertility room space temperature is 12~14
DEG C, temperature is 14~21 DEG C between bottle, and humidity is 65~75%, CO2Concentration is 3000~4000ppm；In mycelia in incubation
Mycelium stimulation is carried out at full bottle 1~2 day, mycelia forbids mycelium stimulation when covering in bottle below 95% area, mycelium stimulation height is with bottleneck distance
2.7 ± 0.1cm keeps charge level smooth after mycelium stimulation, and charge level is rinsed well；Again needle mushroom thallus grow bottleneck height 1.5~
Inserted sheet is carried out when 2.5cm.
9. the production method of selenium-rich gold needle mushroom as described in claim 1, which is characterized in that the harvesting in the step 7 is
It is carried out when the height of needle mushroom thallus is 16 ± 1cm.
10. a kind of selenium-rich gold needle mushroom that the method by as described in any one of claim 1~9 produces.
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