KR102113852B1 - Method for artificial incubation of Zophobas atratus Fabricius - Google Patents

Abstract

The present invention relates to a method of artificial hatching of the American king.
The artificial incubation method of the American king meal of the present invention includes (a) hatching temperature of 29 ° C to 30 ° C, (b) daily conditions of 12L: 12D to 15L: 9D, (C) roughness of 600 to 1,800 or less under the conditions of Lux. It is characterized by hatching the eggs of the king and the king.
According to the present invention, mass production is possible as the optimum hatching conditions of the American kingfish are identified, and it is possible to stably plan production, thereby enabling the insect industry to increase the income and increase income, and at the same time, it can be easily used in farms. Therefore, it is expected to increase labor efficiency.

Description

Method for artificial incubation of Zophobas atratus Fabricius}

The present invention relates to a method for artificial hatching of the American king's giraffe, and more particularly, to a method for artificial hatching of the American king's giraffe through adjustment of a specific temperature, photoperiod, and illuminance to increase the hatching rate in a short time. .

Insects are one of the important roles in the natural ecosystem that are used as animal feed, because many insect species are nutritionally rich. In Korea, it has been used as a feed for pets for more than 10 years, and has started breeding in insect lovers and zoos. Recently, as a feed insect, a number of advantages have led to the growth of farms that multiply for industrial purposes.

In particular, the insect used for animal feed, which is widely used in the past, is the insect of the family Pegasus ( Tenebrionidae ). Several studies have been conducted on the growth characteristics, artificial breeding methods, and artificial feed of these insects.

Related prior arts include Korean Registered Patent No. 10-1660379 (How to shorten the development period of brown larvae), Korean Patent Publication No. 10-2017-0052128 (Feed composition for breeding larvae and larvae), Korean Registered Patent No. 10-1508742 (Jelly-like composition for supplying water to larvae and larvae and a method for manufacturing the same) are known.

Although several studies have been conducted on the beetle and insects, most of them mention the developmental characteristics of the larva or adult of the brown beetle. However, an accurate study has not yet been conducted on the optimum incubation conditions to increase the hatching rate.

An object of the present invention is to provide an artificial incubation method of an American king jerk that can be mass-produced while easily using it at a farm by identifying optimal hatching conditions of an American king jerk.

In order to achieve the above object, the artificial incubation method of the American king meal is (a) hatching temperature of 29 ° C to 30 ° C, (b) daily conditions of 12L: 12D to 15L: 9D, (C) roughness exceeding 600 ~ Characterized in that the hatched eggs of the American king feathers are hatched under the conditions of 1,800 or less, more preferably, the eggs of the American king feathers are hatched at an incubation temperature of 30 ° C., or hatched under the daily conditions of 12L: 12D, or 1,800 Lux It is good to hatch under the illumination condition of.

When the incubation conditions are applied, the eggs of the American king-eater exhibit an incubation rate of 80-90% for 5-7 days, and are characterized by a high incubation rate in a short period of time.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above.

According to the present invention, mass production is possible as the optimum hatching conditions of the American kingfish are identified, and it is possible to stably plan production, thereby enabling the insect industry to increase the income and increase income, and at the same time, it can be easily used in farms. Therefore, it is expected to increase labor efficiency.

1 is a graph showing the hatching rate of American kings by hatching temperature.
Figure 2 is a graph showing the hatching rate per day of the American king meal according to the hatching temperature.
3 is a graph showing the hatching rate of the American king by Gwangju period.
Figure 4 is a graph showing the hatching rate per day of the American king meal according to the Gwangju period.
Figure 5 is a graph showing the hatching rate of the American king by illuminance.
Figure 6 is a graph showing the hatching rate per day of the American king meal according to the roughness.

Hereinafter, the present invention will be described in detail, and detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

In order to achieve the above technical problem, the present invention has been studied several times in order to find the hatching conditions of the American kingfish that can increase the hatching rate in a short period of time, (a) hatching temperature of 29 ℃ to 30 ℃, (b) long-term conditions 12L : 12D to 15L: 9D, (C) It was confirmed that the eggs of the American king larvae exhibited a high hatching rate in a short period of time in an environment of 600 to 1,800 or less.

Hereinafter, an artificial incubation method of the American king meal of the present invention will be described in detail as follows.

All insects are significantly affected by the developmental state or hatching state depending on conditions such as temperature, photoperiod, and illuminance applied during artificial breeding, and the effects on each stage of development are different.

Thus, in the present invention, the hatching temperature of 29 ° C to 30 ° C, (b) daily conditions of 12L: 12D to 15L: 9D, and (C) illuminance exceeding 600 to 1,800 or less incubated in an environment of Lux when hatching eggs of an American king meal Characterized in that, as a specific example, it was confirmed that the eggs of the American king-eater showed high hatching rates in a short period of time while the hatching condition was good under a hatching temperature of 30 ° C or a daily condition of 12L: 12D or an illuminance condition of 1,800 Lux. If the hatching rate falls rather than the above conditions, it has been confirmed that the hatching conditions are indispensable conditions that must be applied when hatching the eggs of the American king.

As a result of confirming the total hatching period and hatching rate of the hatched Native American Pedigree, it shows a hatching rate of 80 to 90% for 5 to 7 days, and it shows a high hatching rate for a short period of time. With this in mind, it is possible to supply materials smoothly as demand increases, and it can be easily used at farms, so it is expected to increase labor efficiency.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

<Example 1> Characterization of temperature suitable for improvement of hatching rate of American king meal

1. Experimental method

1) Experimental insects and numbers: 13G eggs (Eggs spawned for 1 day at 30 ℃, 65% R.H.) of the American King's Wing, Rural Development Administration.

2) Experiment temperature: 25 ℃, 27 ℃, 30 ℃, 33 ℃

3) Survey method and period: 1 survey per day, 10 days from the start

4) Breeding condition: Humidity: 65 ± 5%, Illuminance: 1800lux, Photoperiod: 9L / 15 (this is a general insect breeding condition)

5) Statistical analysis: Chi-Square test, X ² = 51.417, df = 3, p = 0.0001

                    Oneway ANOVA test, F (3,8) = 8.020, p = 0.009

2. Experimental results

As a result of the experiment, it was shown in FIGS. 1 and 2 and Tables 1 and 2 below.

<The number of hatcheries per day according to temperature> Temperature
(℃) Hatching days (days) Total hatching number
(Mari) 4 days 5 days 6 days 7 days 8 days 9 days 10 days 25 - - - 13 88 20 8 129/200 27 - - 26 116 20 - - 162/200 30 - 54 119 9 - - - 182/200 33 8 115 9 One - - - 133/200

<Daily hatching rate per day according to temperature> Temperature
(℃) Hatching days (days) Total hatching rate
(%) 4 days 5 days 6 days 7 days 8 days 9 days 10 days 25 - - - 6.5 44.0 10.0 4.0 64.5 27 - - 13.0 58.0 10.0 - - 81.0 30 - 27.0 59.5 4.5 - - - 91.0 33 4.0 57.5 4.5 0.5 - - - 66.5

As shown in Figures 1 and 2 and Tables 1 and 2, as a result of investigating the hatching rate of the American king meal at four temperatures such as 25 ° C, 27 ° C, 30 ° C, and 33 ° C, 30 ° C was the highest at 91.0%, followed by It was confirmed that the hatching rate in the order of 27 ° C 81.0%, 33 ° C 66.5%, and 25 ° C 64.5% was significantly affected by temperature.

In addition, as a result of examining the daily hatching rate according to the temperature, the lower the temperature, the longer the hatching days tended to be. That is, 25 ° C hatched between 7-10 days, 27 ° C 6-8 days, 30 ° C 5-7 days, and 33 ° C 4-7 days, and 30 ° C showed a hatching rate of 86.5% between 5-6 days. Was confirmed. In conclusion, it is appropriate to store the eggs of the American king at 29 ~ 30 ℃, and more preferably, at 30 ℃ to best hatch the incubation of the American king and the hatchery.

<Example 2> Characterization of the Gwangju period suitable for improving the hatching rate of the American king meal

1. Experimental method

1) Experimental insects and numbers: 14G eggs (American eggs spawned at 30 ° C, 65% R.H for 1 day) at the Rural Development Administration, on the second day of spawning, 100 per test section, 3 repetitions

2) Experiment photoperiod: 9L / 15D, 12L / 12D, 15L / 9D

3) Survey method and period: 1 survey per day, 7 days from the start

4) Breeding condition: Temperature: 30 ℃, Humidity: 65 ± 5%, Illuminance: 1,800lux

5) Statistical analysis: Chi-Square test, X ² (2) = 1.655, p = 0.437

                    Oneway ANOVA test, F (2,6) = 0.535, p = 0.611

2. Experimental results

As a result of the experiment, it was shown in FIGS. 3 and 4 and Tables 3 and 4 below.

<The number of hatcheries per day according to the light cycle> Gwangju Period Hatching days (days) Total hatching number
(Mari) 4 days 5 days 6 days 7 days 9L / 15D 3 171 63 - 237/300 12L / 12D 20 210 22 - 252/300 15L / 9D - 135 110 - 245/300

<Daily hatching rate per day according to the light cycle> Gwangju Period Hatching days (days) Total hatching rate
(%) 4 days 5 days 6 days 7 days 9L / 15D 1.0 57.0 21.0 - 79.0 12L / 12D 6.7 70.0 7.3 - 84.0 15L / 9D - 45.0 36.7 - 81.7

As shown in Figures 3 and 4 and Tables 3 and 4, the temperature was fixed at 30 ° C, and then, as a result of examining the hatching rate of the American kingfish against three photoperiods such as 9L / 15D, 12L / 12D, and 15L / 9D, 12L / 12D was the highest at 84.0%, followed by 15L / 9D, 81.7%, 9L / 15D, and 79.0%.

In addition, as a result of examining the daily hatching rate according to the Gwangju period, in the case of the 12L / 12D sphere, hatching began on the 4th day and 70% of the hatching occurred on the 5th day. 9L / 15D showed a similar trend, but it was confirmed that the hatching rate was about 5% lower. In conclusion, 12L / 12D ~ 15L / 9D are suitable for the Gwangju group, which is suitable for raising the hatching rate of 80% or more, and more preferably 12L / 12D is the most suitable.

<Example 3> Characterization of roughness suitable for improvement of hatching rate of American king meal

1. Experimental method

1) Experimental insects and numbers: 14G eggs (American eggs spawned for 1 day at 30 ℃, 65% R.H) of the American king's nest on the 2nd day of spawning, 70, 100, 50, 3 repeats per test section

2) Experimental illuminance: 600 Lux, 1,000 Lux, 1,800 Lux, 3,000 Lux

3) Survey method and period: 1 survey per day, 7 days from the start

4) Breeding conditions: Temperature: 30 ℃, Humidity: 65 ± 5%, Photoperiod: 9L / 15D

2. Experimental results

As a result of the experiment, it was shown in FIGS. 5 and 6 and Tables 5 and 6 below.

<Daily hatched number of American kings according to illumination> Roughness
(Lux) Hatching days (days) Total hatching number
(Mari) 4 days 5 days 6 days 7 days 600 19 104 52 - 175/220 1,000 17 103 59 - 179/220 1,800 16 103 62 - 181/220 3,000 - 47 105 - 152/220

<Daily hatching rate per day according to roughness> Roughness
(Lux) Hatching days (days) Total hatching rate
(%) 4 days 5 days 6 days 7 days 600 8.6 47.3 23.6 - 79.5 1,800 7.3 46.8 28.2 - 82.3 3,600 - 21.4 47.7 - 69.1

As shown in Figures 5,6 and Tables 5 and 6, after fixing the temperature to 30 ℃, 600 Lux, 1,800 Lux, 1,000 Lux, 3,000 Lux The result of investigating the total hatching number of the King of the United States for 4, 1,800 Lux It was found to be the highest, followed by 1,000 Lux, then 600 Lux, and then 3,000 Lux.

In addition, there is no difference between 1,800 Lux and 1,000 Lux in the total hatching number. As a result of examining the daily hatching rate according to the three illuminances of 600 Lux, 1,800 Lux, and 3,000 Lux, in the case of 600 Lux and 1,800 Lux, hatching starts from the 4th day. It started to hatch and hatched within 3 days. On the other hand, 3,000 Lux hatched from the 5th day of the 1st day and hatched in 2 days.

In conclusion, the roughness suitable for increasing the hatching rate of the American Great Dipper is more than 600 to 1,800 Lux or less, and more preferably 1,800 Lux.

Summarizing the developmental characteristics of the American king-fowl that were investigated in this way, it was confirmed that the eggs of the American king-fed showed 80-90% hatching rate for 5-7 days through the specific hatching conditions, indicating a rapid hatching rate in a short time. Could.

Based on the above results, mass production is possible in a short time as the optimum incubation conditions of the American kingfish are identified, and it is possible to stably plan production, thus enabling the insect industry to activate and increase income, and at the same time, it can be easily used by farms. It can be expected to increase labor efficiency.

The present invention has been described with reference to preferred embodiments, and those of ordinary skill in the art to which the present invention pertains may implement embodiments in different forms from the detailed description of the present invention within the essential technical scope of the present invention. Will be able to. Here, the essential technical scope of the present invention is indicated in the claims, and all differences within the equivalent range should be interpreted as being included in the present invention.

Claims (5)

(a) hatching temperature 30 ° C;
(b) daily conditions 12L: 12D; And
(C) hatching the eggs of the American Great Dipper under conditions of roughness 1,800 Lux; Including,
Characterized by the eggs of the American king-eater, which show an incubation rate of 80-90% for 5-7 days,
The method of artificial hatching of the American king and giraffe.

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