KR101153456B1 - Rearing method of lycaena dispar in a room condition - Google Patents

Abstract

The present invention relates to a method of breeding indoors of the large scarlet beetle butterfly larvae overwintering, more specifically, hatched large scarlet beetle larva 17.5 ~ 30 ℃, long-term conditions (LD 14: 10h) It is possible to breed in, and dormant induction conditions for indoor passage breeding method that can induce dormancy under the condition of 20 ℃, LD 10: 14h or less for long-term storage.

Description

REARING METHOD OF LYCAENA DISPAR IN A ROOM CONDITION}

The present invention relates to a method of breeding indoors of the large scarlet beetle butterfly larvae overwintering, more specifically, hatched large scarlet beetle larva 17.5 ~ 30 ℃, long-term conditions (LD 14: 10h) It is possible to breed in, and dormant induction conditions for indoor passage breeding method that can induce dormancy under the condition of 20 ℃, LD 10: 14h or less for long-term storage.

In general, the Greater Scarlet Butterfly is a member of the Greater Butterflies and is one of the largest species among the Scarlet Butterflies and has been loved by many collectors for its beautiful insects. In Britain, in particular, it has been reported to have failed three times due to extinction due to habitat destruction and reckless collection. Adults are known to occur three times a year in Korea, but the exact ecology has not been determined. The female lays on the leaves of the grasshopper, a food plant, and overwinters as larvae. The larvae feed on plants such as the ridiculous, the true ridiculous, and the ridiculous.

Large scarlet beetle butterfly is known to occur three times a year in Korea. It is a dormant caterpillar from late November to February to March of the next year, and it starts feeding again in a certain warm spring. It is called conditional diapause.

Therefore, there is a need for a breeding method that takes into account the physiological and ecological characteristics of the scarlet beetle butterfly, which can be supplied at any time through the dormancy induction by the low temperature single condition and the dormant breakage by the low temperature treatment. It is emerging.

Accordingly, an object of the present invention is to find the optimal temperature for the development of larvae after hatching, to induce dormancy, and to evacuate the low-temperature treatment period and low-temperature treatment temperature for breeding indoor subculture of large scarlet butterfly By breeding dysfunctional butterfly larvae, it provides a breeding method for breeding indoors all year round.

The present invention, in order to achieve the above technical problem, to find a suitable food source of larvae of large scarlet beetle butterfly, characterized by subcultures of large scarlet beetle butterfly by finding the optimal conditions and dormancy induction, breakable conditions for development do.

 Therefore, the present invention provides a method of indoor breeding of scarlet beetle butterfly, the step of obtaining the hatching larvae of scarlet beetle butterfly; Breeding the hatching larvae; Dormant the larvae bred; Dormant breakdown of the dormant-induced larvae; Solubilizing and allegorizing the dormant larvae; It provides a method of indoor breeding of scarlet beetle butterfly comprising; and the step of naturally spawning the adult female.

The host plant of the large scarlet beetle butterfly may be at least one or more in the group consisting of the squirrel, true squirrel and stone squirrel.

In addition, the step of breeding the hatching larva may be made in 17.5 ~ 30 ℃, long day conditions (14L: 10D).

In addition, the step of breeding the hatching larva may be made at 20 ℃, light conditions 14L: 10D.

In addition, the dormant inducing step may be made at 15 ~ 20 ℃, light conditions 8L: 16D to 12L: 12D.

Alternatively, the dormant inducing step may be performed at 20 ° C. and light conditions of 10 L: 14D.

In addition, the dormant break step may be cold treatment 15 to 20 days after the start of the dormancy.

In addition, the dormant break step may be a low temperature treatment of 3 ~ 8 ℃.

Alternatively, the dormant breakage step may be a low temperature treatment for 60 days at 8 ℃.

In addition, the scattering may be performed at a male and female ratio of 3: 6.

In addition, the scattering step may be made at 23 ~ 27 ℃ and relative humidity 62.5 ~ 87.5%.

In other words, hatched large scarlet butterfly can be bred under the conditions of 17.5 ~ 30 ℃ (light condition LD 14: 10h), and under 15 ℃, even if it is long-term condition, it enters dormancy. It is possible to breed at the above temperature. In addition, in order to make dormant larvae under the conditions of 20 ℃ or less under the conditions of LD 10: 14h, the larvae dormant rate is 92% or more and the dormancy induction period is short, which can induce the most efficient dormancy.

In addition, in order to store dormant larvae at low temperature, it was confirmed that it was best to develop the dormant break treatment at 8 ° C. for 60 days by putting together the conception so that the scarlet larvae larvae could hide their bodies.

Figure 6 illustrates a year-round indoor breeding system of the large scarlet beetle butterfly of the present invention. With reference to Figure 6 looks at the breeding method of large scarlet beetle butterfly.

Eggs of large scarlet butterfly usually hatched 3 to 5 days after spawning.

Larvae of the Great Scarlet Butterflies were about 10-30 days. Using a breeding container with a diameter of 10 cm and a height of 4 cm, 1 to 3 ages were kept at a breeding density of 5 birds / container, and when 4 years old, they were expanded to 1 / container. Kimwipes were soaked in order to retain moisture in the container during the larval period, so as not to dry. After incubation, the host plants were replaced every 2 to 3 days until the 3rd age. At this time, the host plants were cleaned and supplied to reduce the damage caused by parasites. In order to induce dormancy, the 4th larvae of large scarlet insufficiency butterfly were placed at 15 ~ 20 ℃ and light conditions of 8L: 16D to 12L: 12D. The large scarlet bowel butterfly showed a dormancy rate of over 92% at 20 ℃ and below LD 10: 14h, and a short induction period of 16 days. The survival rate was the highest after low temperature treatment (8 ℃, 60 days) after 15-20 days of larvae's dormancy.

The pupal stage of the large scarlet beetle butterfly was about 6 ~ 16 days, and the conditions of breeding container and breeding density were the same as the larval period.

The chrysalis of the great scarlet beetle butterfly became an adult, and it took 1 hour for the scarlet beetle butterfly to spread its wings and dry out completely. A large scarlet butterfly was provided with a male and female ratio of 3: 6 to 100 cm × 100 cm × 200 cm of greenhouse thread with host plants having a height of 50 cm. The large scarlet beetle was spawned 4-5 days after the allegory.

The present invention reveals the physiological and ecological characteristics of large scarlet butterfly butterfly by staging the proper host plant, growth characteristics, dormancy induction and breakage conditions of the large scarlet butterfly butterfly can be stably grown indoors.

Hereinafter, the year-round indoor passage breeding method of the large scarlet beetle butterfly according to the present invention with reference to the embodiment will be described in more detail.

Example 1 Identification of Appropriate Food Sources for Larva Butterfly

Proper food sources were investigated for indoor breeding of large scarlet-flying butterfly larvae. The developmental characteristics of three host plants, Soris, Chamsori, and Solsori, were investigated.

Results for the proper food source of the large scarlet beetle butterfly is shown in Figure 1 and Table 1 below.

[Table 1] Survival rate of large scarlet tortoise butterfly by host plants

U.S. survival rate (%) Dissolution rate
(%) Allegory
(%) 1st age 2nd 3rd 4th A bullshit 100.0 100.0 100.0 93.8 93.8 87.5 Sounder 100.0 100.0 100.0 86.7 86.7 86.7 Stoner 100.0 100.0 100.0 94.9 94.9 76.9

As shown in Figure 1 and Table 1, all three host plants did not show a significant difference in the development period, pupae weight of the larva and pupa, and did not show a significant difference in the survival rate. Therefore, all three host plants were able to breed large scarlet beetle butterfly.

Example 2 Developmental Titration Temperature of Large Scarlet Failure Larvae

The proper temperature for larval rearing was examined for the indoor rearing of the large scarlet-flying larvae. The hatched larvae were treated at intervals of 2.5 ° C. up to 17.5-30 ° C., respectively.

Growth characteristics according to the temperature of the large scarlet butterfly are shown in Figure 2 and Table 2.

[Table 2] Survival rate and dormancy rate of large scarlet butterfly

Temperature (℃) Dissolution Rate (%) Fable Rate (%) Sleep rate (%) 15 - - 72 17.5 100.0 72.7 20 98.1 94.2 - 22.5 94.6 82.1 - 25 92.3 84.6 - 27.5 98.3 81.4 - 30 97.5 85.0 -

As shown in FIG. 2 and Table 2, the larvae of the scarlet beetle butterfly showed a tendency to grow faster as the temperature increases. The larval period was about 29 days at 17.5 ° C, 11 days at 30 ° C, pupa was 16.3 days at 17.5 ° C, and 6.3 days at 30 ° C.

Example 3 Determination of Dormant Induction Condition

In order to induce the dormancy of the large scarlet-flying butterfly larvae, the larvality of the larvae was investigated under light conditions (14, 12, 10, 8L) and temperature conditions (25, 20, 15 ℃).

The dormancy rate and dormancy induction period of the large scarlet failure butterfly are shown in FIGS. 3A and 3B.

As a result of the test at each temperature condition, as shown in FIG. 3a, the dormancy rate was higher than 92% under the condition of LD 10: 14h at 20 ° C, and as shown in FIG. 3b, the dormancy induction period was also about 16 days. It tends to be short. At 15 ℃, the dormancy rate was as high as 72% even in the long day condition of LD 14: 10h.However, in order to artificially induce sleep, the light condition at 20 ℃ is advantageous under LD 10: 14h. It was confirmed that.

Example 4 Determination of Low Temperature Treatment for Dormant Breakage of Larvae of Scarlet Swallowtail Butterfly

5, 10, 15 and 20 days from the time when the larvae hatched into hatched larvae at 20 ° C and LD 8: 16h were incubated to investigate the low-temperature treatment time After 30 days, low temperature treatment (8 ° C., 60 days) was performed, and survival rates were examined.

Survival after large scarlet failure butterfly dormancy was as shown in FIG.

Survival of large scarlet-fly butterfly larvae was taken out after 60 days after low temperature treatment at 8 ° C after 5-30 days in low temperature single conditions (20 ℃, LD 8: 16h) after dormancy. As shown in Figure 4, the highest survival rate was 15 to 20 days. Therefore, it was confirmed that the optimal time for the low-temperature treatment of scarlet beetle butterfly was 15-20 days after dormancy.

Example 5 Determination of Low-Temperature Treatment Temperature of Large Scarlet Butterfly

Survival and development period, solubilization rate, and allegory rate were investigated after 60 days of low temperature treatment at 3 ℃, 5 ℃, and 8 ℃, respectively, in order to identify the optimal temperature for dormant breakdown of large scarlet butterfly.

Table 3 shows the developmental characteristics according to the cold storage temperature of large scarlet butterfly.

[Table 3] Developmental characteristics according to cold storage temperature

process
Temperature % Survival after dormancy Larval Development Period (Days) pupa
Development period (days) Dissolution rate
(%) Weight
(g) Allegory
(%) 4th 5th age Non-dormant - 3.3 4.9 7.2 86.7 0.196 86.7 3 ℃ 75 4.0 5.5 8.3 50.0 0.269 33.33 5 ℃ 66.7 4.6 5.0 9.6 41.7 0.272 41.7 8 ℃ 91.7 3.3 4.6 9.4 50.0 0.259 50.0

As shown in Table 3, as a result of the investigation, the survival rate of the larvae was 91.7%, confirming that 8 ° C was the highest, and the allegory rate also confirmed that 8 ° C was the highest. Therefore, it could be confirmed that the most suitable cold treatment temperature condition for growing larvae was 8 ° C. In addition, in the case of non-dormant individuals, the size of the pupa was about 1.4 times larger than that of the dormant entity.

Example 6 Investigation of Scattering Effects of Host Scarlet Butterfly on Host Plants

In the greenhouse 100cm × 100cm × 200cm (height), the spawning plants were placed in the pots of 50cm in height for the spawning plants and 50s in height. Male and female ratio was performed at 3: 6.

Scattering patterns of the scarlet beetle butterfly are shown in Figures 5a and 5b.

As shown in FIGS. 5A and 5B, the results of the scattering patterns of the scarlet beetle butterfly showed a similar tendency in all of the pterosaurs and the pterosaurs. Showed a tendency to take.

As described above, the present invention has been described based on the preferred embodiments, but these embodiments are intended to illustrate the present invention, not to limit the present invention, so that those skilled in the art to which the present invention pertains can perform the above without departing from the technical spirit of the present invention. Various changes, modifications or adjustments to the example will be possible. Therefore, the protection scope of the present invention should be interpreted as including all such variations, modifications or adjustments.

1 is a graph showing the growth characteristics of large scarlet beetle butterfly according to the host plant.

Figure 2 is a graph showing the developmental characteristics of large scarlet-fly butterfly larvae by temperature.

Figure 3a is a graph showing the dormancy rate of large scarlet butterfly by photoperiod and temperature.

Figure 3b is a graph showing the dormant induction period of the large scarlet dysfunction butterfly by photoperiod and temperature.

Figure 4 shows the post-dormant survival rate of the large scarlet torsion butterfly according to the low temperature treatment on the basis of the start of the dormancy.

5A is a graph showing the number of scattering of scarlet beetle butterfly against the oleander.

Figure 5b is a graph showing the number of scattered large scarlet butterfly for the stone wool.

6 is a year-round indoor breeding system of the large scarlet beetle butterfly.

Claims (11)

As the indoor breeding method of the large scarlet beetle butterfly, Obtaining hatching larvae of large scarlet butterfly; Breeding the hatching larvae; Dormant the larvae bred; Dormant breakdown of the dormant-induced larvae; Solubilizing and allegorizing the dormant larvae; And Indoor breeding method of large scarlet beetle butterfly; comprising the step of naturally spawning the fable adult. The method of claim 1, The host plants of the scarlet beetle butterfly is at least one or more in the group consisting of the squirrel, squirrel and stone squirrel. The method of claim 1, The breeding step of the hatching larva is 17.5 ~ 30 ℃ and long-term condition of 14L: 10D large breeding scarlet butterfly made indoor. The method of claim 3, The step of breeding the hatching larvae indoor breeding method of large scarlet beetle butterfly made in 20 ℃ and light conditions 14L: 10D. The method of claim 1, The dormant induction step is a large scarlet beetle indoor breeding method made in 15 ~ 20 ℃ and light conditions 8L: 16D to 12L: 12D. The method of claim 5, The dormancy inducing step is indoor breeding method of scarlet beetle butterfly made in 20 ℃ and light conditions 10L: 14D. The method of claim 1, The dormant break step is indoor breeding method of large scarlet beetle butterfly low temperature treatment 15 to 20 days after the start of dormancy. The method according to claim 1 or 7, The dormant break step is the indoor breeding method of large scarlet beetle butterfly low temperature treatment of 3 ~ 8 ℃. 9. The method of claim 8, The dormant break step indoor breeding method of large scarlet beetle butterfly low temperature treatment at 8 ℃ 60 days. The method of claim 1, The spawning step is indoor breeding method of scarlet beetle butterfly made of male and female ratio of 3: 6. The method according to claim 1 or 10, The spawning step is indoor breeding method of scarlet beetle butterfly made at 23 ~ 27 ℃ and relative humidity 62.5 ~ 87.5%.

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