KR101922418B1 - Artificial breeding method of Meganoton scribae - Google Patents

Abstract

TECHNICAL FIELD The present invention relates to a method for artificial rearing of a rat. According to the present invention, as a method of artificial rearing at the time of rat shaking is provided, a standardized rearing technology system capable of easily raising at the farmhouse is established by establishing the annual rearing system at the time of rat shaking, It can contribute to improvement.

Description

{Artificial breeding method of Meganoton scribae}

The present invention relates to a method for artificial breeding of Meganoton scribae , and more particularly, to a method for artificial rearing of a rat in which breeding is possible through artificial rearing under a specific environmental condition .

Countries around the world are carrying out national management of biological resources through national key management institutions, and insects are one of the future alternative foods recommended by the United Nations. Insects are proteins that are more than 50% of body weight, and are rich in minerals and amino acids, and are used for food, livestock, poultry, fish, and pets. In recent years, various functionalities and nutrient sources have been continuously studied in order to utilize insects industrially (Choi, Jee-Young, 2015). Among these insects, Pakchigi is an insect belonging to a large species among the pteridophytes, and the wings are narrow compared to the size, and the trunk is thick. The shape and color of the wings varies. There are 58 known species in Korea. It can be seen in various places such as mountains and plains. It is a moth that comes after 1 or 2 hours after turning on a lamp.

Manduca sexta , which is one of the varieties of pakchigi in foreign countries, is widely used for experimental purposes and many scholars expect that it will be used not only for experiential learning but also as a future food substitute resource. However, Manduca sexta is an erectile moth that does not live in Korea, and it is often insect pest problem due to wide range of food plants such as tomatoes and branches. Therefore, Manduca sexta is not suitable for domestic introduction. Among the domestic species of Manduca sexta , there are Meganoton scribae . Although it is known that mice can eat sesame seeds, it is more suitable to feed as a pet, with less concern about insect pests because humans eat plants directly rather than fruit vegetables, and it is more likely to be used as food or edible insects. The rat is a insect of the lepidoptera, and has a body length of 45 mm and a wing length of 100 mm. The body is gray overall. A black frame surrounds the edge of the chest, and the color around the center and edge of the abdomen is dark brown. The front side of the fore wings is gray and there are black stripes everywhere. The hind wings are dark brown. It is very similar to that of a rat, but it is easy to distinguish it because the fore-wing's black pattern is thick. Adult, larvae, pupae, and adults are transformed into feathers. Adults are commonly seen in the mountains in summer and occur once a year. The ecological studies on the rat eruption in Korea, China, Japan, Taiwan and others are insufficient in comparison with the other eruptive moths with much ecological conditions. Accordingly, the present inventors completed the present invention by confirming the feed, temperature, humidity, etc. required for optimal breeding of mouse spp. While trying to develop an artificial breeding method capable of breeding rat spp.

Korean Patent Laid-Open No. 10-2000-0067105 (Name of invention: Feed composition for moths for moths and method for collecting moths using the moths, applicant: Korea, published on November 15, 2000) Korean Patent Laid-Open No. 10-2011-0078610 (Name of the invention: Artificial feed composition for breeding and method of indoor mass rearing of the moths in the deodorization by the same, applicant: Suh Ho Kim, Publication date: July 07, 2011)

 Choi, Jee-Young, A Study on the Specification and Specification of Insect Breeding, National Academy of Agricultural Science, 2015.  Establishment of indoor passaging method for moths, including ginseng, Kwangyu, Rural Development Administration, 1999.  Kim Jae-keun, Zhang Hongxing as a Feeder of Endangered Vegetable Wild Vegetables, Journal of Wetlands Research, 2015, 17 (2), 113-117.  A Study on the Life History of Green Mushik City, Yeo, Sang Duk, 1995, 34 (4), 300-307.

It is an object of the present invention to provide an artificial rearing method for rearing during the year of rat eruption.

The present invention relates to a method for artificial rearing of a rat, wherein the larva, pupa, or adult of the rat is cultivated under conditions of a temperature of 20 to 25 ° C, a light period of 13: 11h to 15: 9h, and a humidity of 50 to 60% to be.

The larvae of the rat larvae were 1 to 5 instar larvae. The mean width of the larvae in the rat larvae was 0.96 mm in the first instar larva, 1.34 mm in the second instar larva, 2.07 mm in the third instar larva, 3.64 mm in the fourth instar larva, Mm.

Among the larvae of the rat, the first to second instar larvae are 50 to 70 mm in diameter and 10 to 20 mm in height; the third larva is a petri dish having a diameter of 90 to 110 mm and a height of 30 to 50 mm; Larvae are preferably raised in a petri dish having a diameter of 110 to 130 mm and a height of 60 to 80 mm or may be raised in a plastic or glass cage having the same height as the respective petri dishes. The larvae can be raised in a Petri dish or a cage. The larva of the mouse can be fed into a petridish or cage with a wood chip before it becomes a pupa.

The larvae of the mouse spp. Can be fed with pellets. A honey can be used as an adult food for the rat.

It is also preferable to cross at a ratio of 1: 2 at the time of crossing the adult in the rat eruption. The solubilization rate of the larvae of the rat at the time of solubilization by the pupa is 85 to 99%. The rate of emergence when the mouse spindle is alienated from adult to adult is 70-90%.

Therefore, in the present invention, more specifically,

(Step 1) Setting the cage of the mouse at 20 ~ 25 ° C, light period 13: 11h ~ 15: 9h and humidity 50 ~ 60%;

(Step 2) Male-adult crossing the adult on the breeding ground to obtain eggs;

(Step 3) hatching the eggs to obtain a larva immediately before becoming a pupa through the first to fifth larva stages; And

(Step 4) Filling the larva immediately before becoming the pupa to obtain a pupa, and cultivating the adult animal with the pupa, and breeding can be carried out.

Hereinafter, the present invention will be described in detail.

The larvae of the rat larvae were 1 to 5 instar larvae. The mean width of the larvae in the rat larvae was 0.96 mm in the first instar larva, 1.34 mm in the second instar larva, 2.07 mm in the third instar larva, 3.64 mm in the fourth instar larva, Mm.

In the present invention, it is preferable that the breeding temperature at the time of mouse eruption is 20 to 25 ° C. Below 20 ° C, it may enter a dormant state. In addition, when the temperature exceeds 25 ° C, the weight of the rat may be decreased or the growth state may become worse. In the 5th instar period, it may escape from the general ecology which is littered with the pupa, and may become a pupa through the sixth instar.

In the present invention, it is preferable that the breeding photoperiod at the time of mouse eruption is 13: 11h to 15: 9h, more preferably 14: 10h. Exposure to these photoperiods can lead to instability or dormancy in the mouse spindle.

In the present invention, it is preferable to maintain the breeding humidity at the time of mouse eruption at 50 to 60%. Humidity exceeding 60% may cause harmful pathogens to the mice, and the survival rate may be lowered. If the humidity is less than 40%, the skin of the larva may dry out and the growth may not be performed well or adults may not be well scattered.

Among the cages for breeding larvae of rat, the 1st to 2nd instar larvae were 50 ~ 70 ㎜ in diameter and 10 ~ 20 ㎜ in height. The 3rd instar larvae were 90 ~ 110 ㎜ in diameter and 30 ~ , 4 ~ 5 larvae are 110-130 mm in diameter and 60 ~ 80 mm in height is recommended to breed in petri dishes. More preferably, the first to second instar larvae have a diameter of 60 mm and a height of 15 mm, a third instar larva has a diameter of 100 mm and a height of 40 mm, and a 4-5 larva has a diameter of 120 mm and a height of 80 Mm in a petri dish, or in a plastic or glass cage having the same height as the bottom surface of each of the petri dishes. The larvae can be raised in a petri dish or a cage, or two birds or one bird, preferably one bird. In this case, in order to breed larvae of various individuals, it is preferable to breed larvae in a cage which has a larger breeding area in proportion to the number of larvae. The larva of the mouse can be fed into a petridish or cage with a wood chip before it becomes a pupa. The bedding absorbs the moisture of the mucous secretion produced by the pupa or regulates excessive humidity in the air.

The cage for the rearing of adult mice can be used for any ventilation cage.

In the present invention, a pellet can be used as a food for a rat. The pellet is characterized by providing a leaf attached to a branch. At this time, the mice are fed with leaves. The only thing that does not provide leaves to mice is because they can be easily damaged by larvae or adult worms when rats are eradicated when they try to remove the larvae or adults holding the cage by forcefully removing them when cleaning the cage. However, when you put the branches, they are hanging on the branches, so you can prevent them from being injured by moving the mouse sprigs while holding the branches. In the present invention, a pellet can be used as a feed for a larva of a rat. At this time, it is desirable to feed 1st to 3rd instar larvae every 2 to 3 days, and after 4th instar, it is better to feed every day since the amount of food is increased. When the adult mouse becomes an adult, the kind of food is changed to flower honey or honey. Therefore, in the artificial rearing condition, the honey can be supplied as food. Since the amount of food to be eaten is remarkably reduced, it is preferable to adjust it according to the consumption amount of food. It is desirable to remove the remaining food after 72 hours of feeding of the rat spp. Because it is not fresh, the fungus is formed or the pathogen can grow.

According to the present invention, when the larva of the mouse is solubilized by the pupa, the solubilization ratio may be 85 to 99%, preferably 90 to 97%. When the mouse spindle is alienated from the pupa to adult, the algebraic rate can be maintained at 70 to 90%.

Accordingly, the present invention provides a method of preparing a food product, the method comprising the steps of: (1) setting a kennel at 20 to 25 ° C, 13: 11h to 15: 9h, and 50 to 60% humidity; (Step 2) Male-adult crossing the adult on the breeding ground to obtain eggs; (Step 3) hatching the eggs to obtain a larva immediately before becoming a pupa through the first to fifth larva stages; And (4) a step of fusing the larva immediately before becoming the pupa to obtain a pupa, and cultivating the pupa with the adult pupa, to breed the mouse pupa. At this time, in the second step, adults are crossed at a ratio of 1: 2 for male adults. The mean values of the larvae of the 1st to 5th instar larvae obtained in the third step are 0.96 mm in the first instar larva, 1.34 mm in the second instar larva, 2.07 mm in the third instar larva, 3.64 mm in the fourth instar larva, and 5.89 mm in the fifth instar larva do. In the third step, among the larvae of the rat, the first to second instar larvae are petri dishes having a diameter of 50 to 70 mm and a height of 10 to 20 mm, the third larva is a petri dish having a diameter of 90 to 110 mm and a height of 30 to 50 mm The larvae of the 4th to 5th orders are raised in a petri dish having a diameter of 110 to 130 mm and a height of 60 to 80 mm or in a plastic or glass cage having the same height as the bottom surface of each petri dish . At this time, the larva can be raised in a petri dish or a cage. It is also possible to put a wood chip into the petri dish or cage before the larva of the mouse is pupae. In the third step, the larva of the rat may be fed with a pellet, and the animal may be fed with honey.

According to the present invention, as a method of artificial rearing at the time of rat shaking is provided, a standardized rearing technology system capable of easily raising at the farmhouse is established by establishing the annual rearing system at the time of rat shaking, It can contribute to improvement. Korean Patent Laid-Open Nos. 10-2000-0067105 and 10-2011-0078610 disclose breeding methods of moths during mosquito moth and off-moth, respectively, but the characteristics of breeding in rat mosquito are described Not only is it characteristic of the feed provided to each moth, but the mating ratios of adult mating adults are different. Although the ecological characteristics of moth moth, moth moth, and green moth have been confirmed in literature such as Sul Gwang Yeol (1999), Kim Jae-keun (2015) and Yeo Duck Duk (1995) On the other hand, the ecological characteristics of the moths of the moth species described in this prior art document are confirmed to be different for each moth, so it is necessary to clarify the ecological characteristics of the moth moth species through the present invention.

Fig. 1 is a photograph showing the ecology of the rat, and the upper right photograph shows the larva of the 5th instar and the lower right photograph shows the larva of the 5th instar immediately before becoming the pupa.
Fig. 2 shows the weight of the male and female pupae when the mice were eradicated according to the temperature.
FIG. 3 is a graph showing the width-to-height comparison of the larval stage of the rat eruption (1 to 5 orders) through the second-degree equation.
FIG. 4 is a graph showing the number of eggs scattered according to the ratio of male and female individuals for crossing of adult mice.
FIG. 5 is a graph showing the survival days of males according to the male to female ratio of adult rats.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

≪ Example 1 > Determination of survival rate by temperature at the time of mouse eruption >

Egg laying eggs were incubated at adult temperature (20, 25, 30 ℃) and grown to adult stage. The light period at each temperature condition was assumed to be LD 14: 10 h, which means that if the photoperiod was adjusted to less than 13 hours during the day, or if the larvae exceeded 15 hours, It is confirmed that it is 14 hours.

The larvae of 1st to 2nd instar were 60 mm in diameter and 15 mm in height. Petridishes of 100 ㎜ in diameter and 40 ㎜ in height were used for the third instar larvae. Petri dishes of 4 ~ 6 larvae were 120 ㎜ in diameter and 80 ㎜ in height And were raised in Petri dishes. For the larvae, pelleted leaves were fed as a food source. The larvae of the 1st to 3rd instar were fed once every 2-3 days, and after 4th instar, the amount of food was increased, so they were fed every day. When the adult rat becomes an adult, it feeds on honey, but the amount of food is significantly reduced, so it is adjusted according to the amount of food consumed. For each petri dish, 1 larva was put into each, and 3 ~ 4 animals were put in the cage.

On the other hand, the food that did not decrease even after 72 hours was removed, in order to prevent old food from getting too dry and not eating well or becoming diseased with mold or germs.

The survival rate of the rat at each time of day was confirmed under these conditions and is shown in Table 1. The adult cage was a circular cage (diameter 30 cm, height 30 cm). The relative humidity in the kennel was maintained at 50 to 60%. Just before he became a pupa, he put wood chips in the bedding and made a pupa space. In this case, when the bedding is fed with masato or soil, it is preferable to put wood chips because the mold is often pierced and the rat spots are dying frequently. In the adult cage, a raspberry leaf with branches for controlling moisture was inserted into the horticultural foam.

Temperature 1st generation Second generation Third generation Fourth 5th 6th pupa imago 20 ℃ 100.0 95.0 95.0 95.0 95.0 - 95.0 85.0 25 ℃ 100.0 100.0 100.0 100.0 96.7 - 96.7 70.0 30 ℃ 100.0 100.0 100.0 100.0 100.0 90.0 90.0 85.0

As shown in Table 1, the mice were able to breed at 20, 25, and 30 ° C temperature, and the kinetics rate (the probability that the larvae ripped out to become pupae) was over 95% ) Was more than 70%. However, at 30 ℃, development of larvae is unstable and development is up to 6th instar. Therefore, 20 ~ 25 ℃ is more preferable for breeding mouse eruption.

≪ Example 2: Comparison of developmental characteristics by temperature at the time of mouse eruption >

Egg laying eggs were grown under the conditions of Example 1 until pupa period.

Temperature 1 day Second generation
(day) Third generation
(day) Fourth
(day) 5th
(day) 6th
(day) larva
Before
(1 to 6 years old
Time sum)
(day) pupa
(day) 20 ℃ 6.3 ± 0.8 5.6 ± 1.0 5.4 ± 0.8 6.8 ± 0.7 14.3 ± 1.1 - 38.3 ± 1.9 26.4 ± 1.2 25 ℃ 5.2 ± 0.6 4.5 ± 0.9 4.0 ± 0.6 4.8 ± 1.2 10.0 ± 1.1 - 28.4 ± 1.5 15.6 ± 0.9 30 ℃ 4.6 ± 0.9 2.9 ± 0.6 3.2 ± 0.7 2.7 ± 0.8 3.8 ± 0.8 7.9 ± 2.5 25.2 ± 3.4 11.5 ± 0.7

As shown in the above Table 2, the period required for larval development in each rat was 38.3 days at 20 ° C, 28.4 days at 25 ° C, and 25.2 days at 30 ° C. As the temperature increased, the larval development period was shortened Is confirmed. On the other hand, at 20 and 25 ℃, there is no 6th instar phase but only 6th instar phase occurs at 30 ℃. This is because the more the 1st instar phase is not suitable for the normal development process. Above 30 ℃, the temperature is high and development is fast, but it is unstable to become a developmental state that can become a pupa. When mice are incubated at a temperature lower than 30 ° C, it takes time to grow, but the weight tends to increase. Therefore, it is concluded that 20 ~ 25 ℃ is suitable for breeding temperature of rat. On the other hand, when the mice are reared below 20 ° C, they enter dormancy and no longer grow. It is important to measure the breeding temperature of mouse spikes because these sleeping temperatures are different for each moth. In addition, the dormant mouse spikes show a tendency to wake up and re-grow when the temperature is increased after refrigeration, but they are not suitable for the persistent mouse spikelet breeding.

≪ Example 3 >

The weight of the pupa was measured according to the rearing temperature at the time when the mice were raised under the conditions of Example 1 and is shown in Fig. Referring to FIG. 2, it was confirmed that at 4.degree. C. and 20.degree. C., 4.8g for females and over 4g for males were exceeded, which proved to be relatively large and stable. However, at 30 ° C, it is confirmed that the growth state is poor due to the small weight of the pupa.

≪ Example 4: Thickness comparison according to the breeding temperature at the time of rat erection >

The larval width of the larvae bred at 25 ° C under the conditions of Example 1 was measured. The larvae of each of the 1st to 5th instar larvae were collected daily to confirm whether or not the larvae were molted. The larval angles of each larvae were fixed by using a transparent paste, fixed with a fixative, Respectively. The measurement results are shown in Fig.

Referring to FIG. 3, the average value of the width is 0.96 mm at the 1st, 1.34 mm at the 2nd, 2.07 mm at the 3rd, 3.64 mm at the 3rd, and 5.89 mm at the 5th. By measuring the width at 25 ° C, insect breeders can easily gauge the larval stage through the size of the width.

≪ Example 5: Scattering characteristics and adult survival time according to the ratio of male and female adults when crossing with adult rats at the time of rat erection>

The mice were divided into three groups: 1: 2 (total 3), 1: 1 (total 4), and 2: 1 (total 3) in a cage (diameter 30 cm, height 30 cm) Mating and scattering characteristics were analyzed according to the ratio of male and female population. At this time, the condition of the cage containing the cage was controlled at a temperature of 25 ° C and a humidity of 50 to 60%, and the food was not supplied separately, but the leaves of the cage were added to control the moisture. At this time, the branches were kept in a floral foam with a moisture content. The daily living and adult survival were investigated and shown in FIGS. 4 and 5.

Referring to FIG. 4, it was confirmed that eggs were scattered at an average of 257.6 eggs at a ratio of 1: 2 in the ratio of male and female individuals, and the egg production rate was significantly decreased at the other ratios.

On the other hand, referring to FIG. 5, when the ratio of male and female individuals is 2: 1, it can be seen that the survival days of males are the shortest as 5.6 days.

Despite the similarity of males and females at 1: 1 and 1: 2 ratios, the 1: 1 egg production rate drops. The higher egg production ratio at the ratio of 1: 2 in male and female ratios seems to be due to the better scattering ability of the rat during the multiple mating.

Claims (11)

(Step 1) Setting the cage of the mouse at 20 ~ 25 ° C, light period 13: 11h ~ 15: 9h and humidity 50 ~ 60%;
(Step 2) In the breeding cage, eggs are placed in a circular cage having a diameter of 30 cm and a height of 30 cm with three birds at an adult ratio of 1: 2, and mated to obtain eggs;
(Step 3) hatching the eggs to obtain a larva immediately before becoming a pupa through the first to fifth larva stages; And
(Step 4) A step of raising the pupa by cultivating the adult pupa before the pupa is completed, obtaining a pupa, and cultivating the adult animal with the pupa,
In the third step,
Feeding larvae with pine needles with twigs,
Among the larvae, the first to second instar larvae are petri dishes having a diameter of 50 to 70 mm and a height of 10 to 20 mm, a third larva is a petri dish having a diameter of 90 to 110 mm and a height of 30 to 50 mm, A petri dish having a diameter of 110 to 130 mm and a height of 60 to 80 mm or a plastic or glass cage having the same bottom surface and the same height as the petri dishes,
In the fourth step,
Before the larvae became pupae, the wood chips were put into bedding in petri dishes or cages,
In the fourth step, the rate of solubilization of the larvae of the rat at the time of solubilization by the pupa is maintained at 80 to 95%, and the rate of algebra when the mouse is alienated from the pupa to adult is maintained at 70 to 90% Artificial Breeding Method of Poultry. The method according to claim 1,
The larvae of the rat larvae were 1 to 5 instar larvae. The mean width of the larvae in the rat larvae was 0.96 mm in the first instar larva, 1.34 mm in the second instar larva, 2.07 mm in the third instar larva, 3.64 mm in the fourth instar larva, Mm. ≪ / RTI > delete delete delete delete The method according to claim 1,
The method of artificial breeding according to the present invention is characterized in that the rodent is fed to the adult of the rat. delete delete delete delete

Images