KR20070106838A - Mass rearing method for staphylinidae and container comprising it - Google Patents

Abstract

A mass rearing method of Staphylinidae and a container of Staphylinidae are provided to enable mass rearing by reducing the fatalities and increasing the breeding rate of Staphylinidae that is a predator of fly. A mass rearing method of Staphylinidae includes: an egg collecting step of putting grown up Staphylinidae into an egg collecting box, and collecting the eggs of the grown up Staphylinidae; a larva breeding step of moving the eggs of the grown up Staphylinidae to a larva breeding box to be hatched and breeding the larva of Staphylinidae until it becomes 2nd grade larva; a last instar larva breeding step; a chrysalis breeding step; a grown up Staphylinidae copulating step; and a step of moving copulating grown up Staphylinidae to an egg collecting box.

Description

Mass rearing method for Staphylinidae and container comprising it}

1 is a schematic view of the egg box or breeding larval breeding box.

2 is a schematic view showing an example of a larval breeding box.

3 is a schematic diagram of the pupae pupae breeding box.

4 is a schematic diagram of a mating box.

5 is a schematic view of a product container.

6 is a photograph of a half-winged adult half-winged family.

Figure 7 is a photograph of the half-winged species larvae half-wings.

<Description of the symbols for the main parts of the drawings>

12, 25, 32, 46, 52: vent 14, 24, 34, 44, 54: lid

16, 26, 36, 42, 56: main body 18, 28, 38, 48: sterile medium

30: handle 50: inner container

58: filler

The present invention relates to a half-wing and mass rearing method and a half-wing family merchandise container, and more particularly, to a method of mass rearing the half-wing family by lowering the mortality rate and increasing the proliferation rate of the fly predatory half-wing family. It relates to a commodity container to contain.

Flies are pests that transmit harmful elements to people and livestock. Such flies cause unsanitary conditions, especially in farms such as pigs and barns, and cause problems such as decreased worker's desire to work, decreased productivity, and transmission of infectious diseases.

Control of flies is achieved by chemical control methods (insecticides), physical control methods (flytraps, sticky traps), biological control (natural).

Conventionally, chemical insecticides were used for the control of flies, and organophosphorus insecticides were used. However, this can remove the adult fly, but it does not work on fly eggs, larva and chrysalis in particular, especially when the fly eggs, etc. present in the animal meal because there is no effect at all should be spraying pesticides There was this. In addition, the insecticide may be harmful to humans and livestock, in addition to flies, there is a problem that causes environmental pollution, such as destruction of the ecosystem. In the case of using the insecticide for a long time, the resistance of the fly becomes stronger, so there is a problem of increasing the amount of use or developing a new highly toxic drug.

In addition to insecticides, flytraps are known as a tool for attracting flies and capturing flies, and short-necked glass bottles allow the flies attracted to the inside through the neck to be trapped in the bottle so that they cannot be recaptured. It is difficult.

In addition, there is a simple method of extinguishing flies using a sticky trap, but in order to use the sticky traps, they have to be hanged on the ceiling. When using the problem was that the range is limited by the influence of the wind.

Among various control methods, there is a method of using natural enemies as a biological control method, which is an eco-friendly control method that can maintain the food chain by restoring the ecosystem. There are two methods of using natural enemies: parasitic natural enemies and predatory natural enemies.

One of the above control methods can not completely control the fly pests, it is effective to use a variety of methods at the same time. In particular, when the biological control method is used as the main method, if the parasitic and predatory natural enemies are used at the same time, the insecticidal effect is increased and the desired control effect can be obtained without using the pesticide at all.

In Korea, mass paralysis and commercialization of parasitic flies was carried out, but there was no successful case of mass propagation, and commercialization was not successful.

Laboratory studies of mass propagation in the half-wing family published overseas have not been commercialized due to the high mortality and low proliferation rates of eggs, larvae, pupa and adults. In the above study, the problem was that it was difficult to achieve the purpose of commercialization due to the reduction in production efficiency due to the high mortality rate. The high mortality rate in the process of mass growth is mainly due to the strong homologous nature of the king's wing and the contamination of larvae and eggs. Therefore, the problem of allograft and contamination of the half-wing family was a problem to be solved in the mass growth of the half-wing family.

In addition, the problems of alleged predation and larvae and eggs of the half-wing family were to be solved not only at the growth stage of the half-wing family, but also at the commercialization stage to stably supply the half-wing family as a product for controlling flies.

Accordingly, the first technical problem to be achieved by the present invention is to provide a method for mass breeding by lowering the mortality rate and increasing the proliferation rate of the flies predatory half-wing family.

The second technical problem to be achieved by the present invention is to provide a commodity container including a half-wing section of a time appropriate for commercialization.

The present invention to achieve the first technical problem,

(a) breeding a half-winged Staphylinidae nymph of a coleoptera predatory flies and fly larvae in a egg box, and eggs that are spawned by the half-winged nymph;

(b) the larval rearing step of breeding the half-winged eggs and eggs from the egg box to the larval rearing box and hatching them through the half-wing and the first-larva larvae until the half-wing and the second-larvae larvae;

(c) breeding stages of the larval larvae rearing part or all of the half-wing and second-larva larvae until the transformation from the larval rearing box to the half-wing and pupa;

(d) chrysalis individual breeding step of breeding the half-wings and chrysalis from the chrysalis breeding box to half-wings and adult;

(e) putting the half-wings and the adult transformed in the pupa object breeding box into a mating box so that the female and male half-wings and the adult are mated; And

(f) It provides a half-wing and mass breeding method comprising the step of transferring the female and male wing and adult in the mating box to the egg box.

According to one embodiment of the present invention, the egg box includes a vent 12, a sterile medium 18 and a sterilizing solution therein, and the egg box may be put into the egg box by putting a pair of vanes and male and female. .

According to another embodiment of the present invention, the egg and the egg collected every two days from the egg box can be collected.

According to another embodiment of the present invention, the larval breeding box includes a vent 25, a sterile medium 28 and a sterile liquid therein, and can be put half wings and 50 to 100 larvae.

According to another embodiment of the present invention, the seed larval breeding box is a barrel containing a vent 12, a sterile medium 18 and a sterile liquid, it can be put half wing and two larvae.

According to another embodiment of the present invention, the pupa individual breeding box is a transparent container containing sand, sterile wood chips, sterile liquid, the vent 32 can be covered with a paper lid 34 in the center portion, One chrysalis can be put in.

According to another embodiment of the present invention, the mating box is a transparent container having the vent 46, it may further include an inner container 50 containing a sterile medium and a sterilizing solution 48 therein.

The present invention provides an opaque half-wing and a product container including a half-wing and second-larva larva produced therein by the half-wing and the mass breeding method to achieve the second technical problem therein and including an air vent 52 at the top. do.

According to an embodiment of the present invention, the vane and the inside of the product container may further include a sterile wood chip, sand, sterile liquid, anti-corruption liquid.

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings and examples, but the present invention is not limited thereto.

Half wing and mass breeding method according to the present invention comprises the steps of (a) breeding a half-wing (Staphylinidae) adults of the coleoptera predatory to the fly eggs and fly larvae in the egg box and breeding the eggs spawned by the half-wings and adults; (b) the larval rearing step of breeding the half-winged eggs and eggs from the egg box to the larval rearing box and hatching them through the half-wing and the first-larva larvae until the half-wing and the second-larvae larvae; (c) breeding stages of the larval larvae rearing part or all of the half-wing and second-larva larvae until the transformation from the larval rearing box to the half-wing and pupa; (d) chrysalis individual breeding step of breeding the half-wings and chrysalis from the chrysalis breeding box to half-wings and adult; (e) putting the half-winged and adult adult in the pupa object breeding box into a mating box so that the female and male half-winged and adult are mated; And (f) transferring the female and male wings and adult insects mating in the mating box to the egg box, and the step of breeding the half-wing family for each fetus, such as adult, larva, species larva and pupa. It features. By the management of such fetuses, the likelihood of allografts is lowered and mortality is lowered. As a result, the rate of proliferation of the half-wing family can be increased. In addition, it is possible to increase the growth rate by using a suitable breeding box for each step and using a disinfectant solution for the purpose of water retention and disinfection.

The above-mentioned egg box may serve as an adult breeding box for breeding half-wings and adults in addition to the purpose of egg harvesting. As shown in Figure 1, the chaeran box is not limited to the material and size, but the lid 14 including the vent 12 in the center of the size of 25 to 30cm in width, 30 to 40cm in height, 15 to 20cm in height The body 16 may include a sterilization medium 18, a sterilization liquid, and the like.

In the egg box, the half- wing ( Creophilus) maxillosus ), pretty little wing ( Philonthus didciodeus ), Golden Black Wings ( Ocypus) gloriosus ), Black-eared Beard Halfwing ( Aleochara) curtula ) and half- wings and adults can be raised.

Of the half-winged and adult, the half-winged adult can be collected outdoors, and then purified by individual breeding or by hen breeding.

The Half- wing, Creophilus maxillosus (Coleoptera: Staphylinidae) is a widely distributed species in the world, as shown in Fig. 6, insects of about 15mm in size to feed the eggs and larvae of the barn and compost. The wangwings feed on most larvae of fly species, and female adults lay 10 to 20 eggs per day to spawn 500 to 1,000 eggs for life.

The hwangwing is (1) distributed throughout most of the country's livestock farms, so the coverage area is wide, (2) the size of the individual is relatively large and the number of spawning is larger than other species, (3) the predation is large, flies eggs, larvae, (4) All the fetuses exist every season, and it is easy to settle wintering insects.

The introduction of the outdoor object group for indoor cage breeding was carried out in the period of June to August, and the outdoor object group was purified for one week. The collection of the king's wings was carried out four times in each survey area between May and August. The number and collection of the king's wings are shown in Table 1. From Table 1, it can be seen that in case of collecting the hwangwing in a humid place, and to use the hwangwing as a fly predator, it is advantageous to radiate the radiation place in a humid place such as a conventional pig manure.

Survey area Money processing method King's wing collection number (adults / larva) Gathering conditions (humidity) Picking place  Pig manure 1  Conventional 5/20 2/12 1/5 0/8  Humidity  Money Buns in Money Buns  Pig manure 2  Sawdust Donsa 0/0 0/0 0/0 0/0  dry  -  Pig manure 3  Scraper 0/0 1/0 0/0 0/0  dry  Swine flour, in a sawdust mixture

In order to prevent contamination of wild populations, the breeding medium can be sterilized and sterilized using a disinfectant solution (Hansol, 1-5% dilution of "Harasol" for animal drinking water). The disinfectant solution may be used for the addition of moisture in the breeding container in addition to the purpose of disinfection. This is because the inside of the breeding vessel can be multiplied by the king half wing if a certain humidity or more.

Fly larvae or fly eggs can be used as food for the larva wing in the egg box, and 20 flies can be supplied per adult larva.

The vent 12 of the chaeran box may have one vent on the lid 14 of the chaeran box. The 1 mm mesh fabric net can be installed in the vent 12, thereby preventing rodents from invading from the outside and facilitating ventilation and humidity control. The sterilization medium 18 may be composed of sterilized sawdust and the like. By using the sterilization medium 18 and the sterilization liquid, it is possible to prevent contamination of adults and eggs. The thickness of the sterilization medium 18 may be 5 ~ 10cm. If the thickness is less than 5cm, the medium dries quickly, making it difficult to maintain the humidity of the egg box, and if it exceeds 10cm, it may be difficult to observe and collect the king's wings or eggs. In addition, the sterilization solution can not only prevent contamination, but also maintain the humidity of the egg box at 50 to 60%. If the moisture content is less than 50%, there is a concern that the egg is not spawned or the number of spawning is small. If the water content exceeds 60%, there is a fear of creating an environment in which spawning is insufficient. When breeding, the lower part increases the moisture content, and the upper part decreases the moisture content so that it can maintain a suitable state for spawning. At the same time harvesting eggs can be harvested by introducing fresh spawning medium. And after collecting the predatory adults, the remaining egg spawn medium can be transferred to the larva rearing box to breed 50 to 100 individuals at the same time.

In the egg box, one pair of male and female adult half wing wings may be inserted to eggs. Wang half wing is highly homologous, but adults and females do not attack or homologize each other, so they can be bred in the same egg box. In addition, the half-winged adults are mated from time to time, so if you raise them in the same box, you can increase the amount of eggs. Badges and eggs harvested every two days from the egg box can be collected. An average of 10 eggs can be collected at one time and a total of 40 or more can be collected. The reason why eggs are collected every two days is because the half-winged eggs spawn 10 eggs for two days and the half-winged eggs feed on the scattered eggs. This is because the eggs need to be collected.

Eggs harvested from the egg box may be transferred to the larval breeding box of FIG. 2, which is the next step, to collectively breed up to 2 larva larvae by 50 for each breeding box. Because of the low likelihood of homophagy until the second larval stage, breeding of the hwangwing does not decrease. The larval breeding box can be bred for about 10 days. The second larvae reared in this way can be packed for the purpose of commercialization of 100-200 individuals. This is because all of the predation and deactivation are the least during the second larval stage, so the fly control effect can be increased by utilizing the larvae during this period. In addition, the cold storage test also reflects the high survival rate of the second larvae to maintain 80% even after 30 days.

The larval breeding box is a container including a lid 24, the main body 26, the vent 25, the sterilization medium 28 and the sterilization liquid, etc., but the size or material is not limited 25 to 30 cm, 30 vertical It can be as small as ~ 40 cm and a height of 15 to 20 cm. In the larval breeding box can be put 50 ~ 100 individuals of Wang half wing larvae. The larva rearing box may use the same structure as the egg box. However, as shown in FIG. 2, the vent 25 may be made to have a better ventilation by making a total of four, two each on both sides of the larva breeding box. The larval breeding box can be put in the sterilization medium 28 and the sterilization liquid to maintain the water content of 20 to 50%. By installing the vent 25, it is possible to smoothly vent and maintain the humidity at a constant level. 1 mm mesh netting can be installed in the vent 25 to prevent the escape of natural enemies, to prevent invasion of pests from the outside, and to facilitate ventilation and humidity control. The sterilization medium 28 may be composed of sterile wood chips and sand. By using a sterile medium 28 and a sterile liquid in the larva rearing box to prevent contamination of eggs and larvae can increase the survival rate of the larvae. The thickness of the sterilization medium 28 may be 5 ~ 10cm. If it is less than 5cm, the medium may dry quickly and there may be difficulty in maintaining humidity. If it exceeds 10cm, it may be difficult to observe the larvae or eggs and collect the larvae. In addition, it is possible to prevent contamination with the sterilization liquid, and to maintain the humidity of the egg phase at 50 to 60%. When the water content is less than 50%, there is a fear that the larvae will not develop enough, and when the water content exceeds 60%, the larvae may fall into the water and die.

Fly larvae or fly eggs can be used as prey of the larval wing larvae in the larva rearing box, and 5 to 10 of the larva per larva wing larvae can be supplied.

In order to use some or all of the second-larvae larvae for breeding can be reared to breeding larvae breeding box two by two to breed about 20 days until pupa. When breeding the wangwing wing larvae of Fig. 7 in the breeding box larvae can be reared separately because the propagation rate of the wangwing wing is reduced due to strong allogenic predation at the time of the larval larvae. The seed larvae breeding box may utilize the chaeran box of Figure 1, as shown in Figure 1 will include a body 16, a lid 14, a vent 12, sterile medium 18 and sterilization liquid, etc. Can be. There is no restriction on the size or material of the breeding larva, but the size of 25-30cm wide, 30-40cm high, 15-20cm high, can use a sealed plastic container having a vent 12 on the top At the bottom of the container can be a mixture of sterile sand and sterile solution. In this way, the water content can be maintained at 20 to 50%. If the moisture content is less than 20%, there is a concern that the growth of the larva is not sufficient, and if it exceeds 50%, there is a fear that the environment for the growth of the larva. In addition, it is possible to install a 1mm mesh cloth net in the vent 12, to prevent invasion of rodents and the like from the outside, it is possible to facilitate the ventilation and humidity control.

Fly larvae or fly eggs can be used as prey of the larval wing larvae in the larval breeding box, and 20 flies can be supplied per adult larvae.

After 20 days in the breeding larvae breeding pupae transformed into pupae, that is, if the pupae breeding individual dogs can be reared about 15 days.

As shown in FIG. 3, the pupa individual breeding box may include a main body 36, a lid 34, a vent 32, a handle 30, and a sterilization medium 38.

The pupa individual breeding box is not limited in size or material, but can be used in a 20ml capacity of a transparent plastic material. The transparent plastic material makes it easy to observe from the outside and easy to handle. The lid 34 of the pupa individual breeding box may be provided with a vent 32 by cutting out only a portion of the central portion with a paper material and attaching a mesh network. The 1 mm mesh cloth net can be installed in the vent 32 to prevent external rodents from invading, and to facilitate ventilation and humidity control. Some of the cut off the role of the handle 30 can be easily opened and closed the lid so that you can easily put the pupae soluble pupa adult can easily pull out. At the bottom of the pupa object breeding box, a sterile medium 38 containing a mixture of sand, sterile wood chips, sterile solution, etc. may be laid to provide a suitable environment for the pupae.

The adult allegory from the pupa individual breeding box can be transferred to the mating box of FIG. 4 to induce daily mating immediately after the allegory. The mating rate can be increased by moving the alleged half-wing family from the pupa individual breeding box to the mating box to allow 10 to 20 individuals to mate in the mating box.

As shown in FIG. 4, the mating box may include a lid 44, a body 42, a vent 46, an inner container 50, a sterile medium 48, and the like. An air vent 96 may be installed on the cap 44 of the mating box to protect the internal fly predators from invasion of the aeration and external rodents. The mating box is not limited in size or material, but may include an inner container 50 inside the sterilized medium 48 and the upper portion of the sealed box made of a transparent acrylic resin. If you put an adult in the mating box, there is a problem of climbing up the wall, so that the sterile medium 48 is installed inside the mating box and the upper box 50 can be installed and the adult can be put therein. . In this case, the thickness of the medium may be 0.1 to 1 cm. The reason is that if the size is less than 0.1cm there is a fear that the role of the medium and in the case of more than 1cm the adult adults may breathe with the medium. The reason for using a transparent material as the mating box is for ease of observation. In addition, if the number of adult mating in the mating box is less than 10 individuals, the adult sex is difficult to discriminate, so the possibility of male and female evenly enters, there is a possibility that the mating rate is lowered, if more than 20 individuals, it is difficult to observe, homologous predation Because there is concern. In the mating box, a pair of females and males mating in the mating box may be transferred to the egg box, and the egg may be subjected to the following stages such as larval rearing. When transferring the adult to the chaeran box can be carried using a cup or tweezers.

As described above, the second larvae reared in the larval rearing box may be packaged as a commodity. The packaging may be a half wing product container and the half wing product container may include a lid 54, a main body 56, and a filler 58 having an air vent 52 thereon as shown in FIG. 5. . The half-winged product container may use an opaque plastic container as a whole. The material is not limited to plastic, but plastic can be cheap and easy to handle. In addition, the use of an opaque material can protect the internal half-wing larvae from sunlight and the like. The vent 52 may be provided with a mesh having a mesh of 1 mm or less to prevent the larvae from escaping. The container can be filled with more than 100 pieces of filling material and Wang half wing larvae. By commercializing in the stage of larvae of the second half with low predation and low vitality, it is possible to minimize the loss of the half wing and at the same time increase the predatory power of the half wing to effectively control flies. The filler 58 may be made by mixing sterile wood chips, sand, sterile liquid and the like, and may be added to the anti-corruption liquid. The sterilization liquid may be used 1 ~ 5% chlorine disinfectant solution and can not only sterilize the medium by mixing the sterilization liquid in the medium, but also maintain the water content in the container at 30 to 50%. The anti-corruption liquid may include a small amount of penicillin or streptomycin and may prevent the occurrence of mold and the like during distribution.

Cold storage may be used to extend the shelf life of the halves. In the case of low-temperature storage, the shelf life can be extended by 30 days if it is carried out under the condition of 65 ± 5% humidity and 0 ~ 4 ℃. King-winged larvae and second-larvae larvae had a survival rate of more than 80% until 35 days.

As described above, according to the present invention can reduce the mortality rate and increase the proliferation rate of flies predatory half-wing family can be used to breed the half-wing family in large quantities can be used for the control of flies, half-wing family allograft It is possible to effectively control flies by providing a commodity container including a half-wing section in which the vitality is kept at the same time and the vitality is maintained.

Claims (9)

(a) breeding a half-winged Staphylinidae nymph of a coleoptera predatory flies and fly larvae in a egg box, and eggs that are spawned by the half-winged nymph; (b) the larval rearing step of breeding the half-winged eggs and eggs from the egg box to the larval rearing box and hatching them through the half-wing and the first-larva larvae until the half-wing and the second-larvae larvae; (c) breeding stages of the larval larvae rearing part or all of the half-wing and second-larva larvae until the transformation from the larval rearing box to the half-wing and pupa; (d) chrysalis individual breeding step of breeding the half-wings and chrysalis from the chrysalis breeding box to half-wings and adult; (e) putting the half-wings and the adult transformed in the pupa object breeding box into a mating box so that the female and male half-wings and the adult are mated; And (f) a half-wing and mass breeding method comprising the step of transferring the female and male half-wings and adults from the mating box to the egg box. The method according to claim 1, wherein the egg box includes an air vent, a sterile medium, and a sterilizing solution therein, and the half wing and the mass breeding method characterized in that the egg box is put into the egg box and the male and female pairs are fed. The half-wing and mass rearing method according to claim 1, wherein the medium and eggs collected from the egg box are collected every two days. The method according to claim 1, wherein the larval breeding box includes a vent, a sterile medium and a sterilizing solution therein, and the half wing and mass breeding method, characterized in that 50 to 100 larvae are added. The method according to claim 1, wherein the larval rearing box includes a vent, a sterile medium and a sterile liquid therein, and half wing and two larvae are introduced. The method according to claim 1, wherein the pupa individual breeding box is a transparent container containing sand, sterile wood chips, sterilizing solution therein, the vents and the half wing and mass breeding method, characterized in that the cover can be covered with a paper lid in the center portion . The method of claim 1, wherein the mating box is a transparent container having a vent, half vanes and mass breeding method further comprising a container including a sterilization medium and a sterilizing liquid therein. An opaque half-wing and commodity container comprising a half-wing and a second-larvae larva produced therein by the half-wing and mass rearing method of claim 1 and including an air vent at the top thereof. The half wing and the product container according to claim 8, further comprising sterile wood chips, sand, sterile liquid, and anti-corruption liquid therein.

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