KR20150062672A - Breeding method of natural enemy Chrysoperla nipponensis for scale insect pest control - Google Patents

Abstract

The present invention relates to a method for breeding lacewings which are a natural enemy of scale insects to repel the scale insects, wherein breeding lacewings which are a natural enemy of scale insects which can be found on fruit tree products and are hard to repel are mass-bred. The method comprises the steps of: discharging lacewing eggs onto a plant in a breeding cage in which feeding aphids are artificially proliferated; maintaining temperature and humidity in the breeding cage for 20-40 days in a regular condition to make about 50% or more of the discharged lacewing eggs eclose into imagoes; collecting eclosed lacewing imagoes in male and female pairs and transferring the same to an egg collecting container; periodically selecting and supplying any one of a sponge wetted with an imago artificial fodder or an alternative feed to the egg collecting container; and collecting eggs spawned from the lacewing imagoes from the egg collecting container and disposing the same in a large breeding container having a ventilation hole to cure the same for a particular period of time.

Description

{Breeding method of natural enemy Chrysoperla nipponensis for scale insect pest control}

The present invention relates to a breeding technique of an indigenous enemy used for biological control of a heating insect pest, and particularly relates to a method for breeding a nematode (C. nipponensis), an indigenous natural enemy of a pest insect, The present invention relates to a method for raising nematode butterfly for controlling insect pests.

In case of heating insect pests occurring in fruit crops, insecticidal effect is low, resistance to medicines is acquired, and new outpatient pests are introduced, etc., there is a problem that a pest insecticide must be sprayed on existing natural enemies even when heat insect pests are generated in environment- .

For example, among the 255 species of pests that are grown in Korea, five kinds of pest worms have been reported, including pest worms, and the pest is a major pest that shows a damage rate of about 5% Soot disease caused by incidence and excretion has been a cause of deterioration of fruit product value.

In addition, since most of the pears are grown in bags, the pod worms settled in the fruit of the bag have difficulty in controlling because they do not come into contact with the medicines. As the pod bugs grow, they secrete white waxy substances to protect the body. Since the drug efficiency is extremely low when the bandit (hatching larvae stage) is missed, development of biological control technology using natural enemies has been urgently required.

These indigenous natural enemies are biological species adapted to the domestic climate and are easy to settle as a biological control factor. They are also part of the technology for controlling insect pests. Especially, when the environment friendly agricultural products production and spraying are difficult, The development of technologies that can be used is urgent.

However, about 30 species have been developed as domestics for domestic pest control. However, commercialized and distributed species are limited to some species, and development of insect pest control agents such as insect pest insects has never been developed. Therefore, it depends on import from foreign countries.

Disclosure of the Invention The present invention has been made to overcome the above-mentioned problems, and an object of the present invention is to provide a method for controlling C. nipponensis, which is an indigenous natural enemy for controlling insect pests such as pears, vineyards, And to provide a breeding method for the breeding.

In order to accomplish the object of the present invention, there is provided a method for producing a food-containing aphid, comprising the steps of:

Maintaining the temperature and the humidity in the breeding cage at a constant condition for 20 to 40 days so that about 50% or more of the radiated nematode eggs are fished with adults;

Collecting a pair of nematocysts of the genus Nematoda with male adults, and transferring them to a packed vessel;

Periodically selectively supplying any one of a sponge or a substitute food impregnated with an adult artificial diet to the container vessel;

The method comprising the steps of: collecting nematode grasshopper eggs scattered by the nematocerospora larvae in a large rearing container having a ventilating port and curing the nematode nematode larvae for a predetermined period of time;

The present invention as described above has an effect of stabilizing the production of eco-friendly agricultural products by farmers and contributing to the increase of exports because it is possible to breed a large amount of nematode nematodes for insect pests such as insect pests.

Further, since the present invention enables biological control by mass rearing of indigenous enemies, the use of pesticides is excluded, and environmental pollution and accumulation of pesticide ingredients in the body can be prevented.

Fig. 1 is a graph showing insect pests and indigenous nematode occurrences of nine species such as insect pests, insects,
Fig. 2 is a graph showing the occurrence of the powdery mite worms in the Gyeonggi area (Anseong)
Fig. 3 is a graph showing seven species including native nematodes and nematode
Fig. 4 is a table showing the developmental characteristics and the predation power of the nematode
FIG. 5 is a graph showing the results of a functional response test
FIG. 6 is a graph showing the distribution of the nematode latent feeding spectra of the nematode flora (nematode)
The left side of FIG. 7 is a photograph of a powdery moth bug, and the right side is a photograph of a close-up photograph of a nymph and adult
Fig. 8 is a graph showing the predation rate of the larvae of the nematode
The left side of Fig. 9 is aphid photograph, the center is a photograph of a flower pear, and the right side is a photograph of a nematode larva
Fig. 10 is a graph showing the feeding response (2013) to cotton aphid of the nematode larvae (second instar larvae)
Fig. 11 is a graph showing the insecticide susceptibility
FIG. 12A is a graph comparing the density change of spring coat worms (spring)
FIG. 12B is a graph showing a comparison of the density change of pear flour worms
FIG. 12C is a graph showing the effect of control of the powdery mildew insect control by the nematode
The left side of Fig. 13 is a photograph with a normal image, and the right side is a photograph
Fig. 14 is a graph showing a comparison of the reported blastosity of each treatment
FIG. 15 is a graph showing a comparison of the quality of the report worm

Hereinafter, embodiments according to the present invention will be described in detail.

A step of inoculating an insect parasitic plant in which a feeding aphid has been proliferated, a step of inoculating a nematode grasshopper egg into a parasite plant, A step of periodically supplying a sponge impregnated with an adult artificial diet; and a step of collecting the nematode grasshopper eggs scattered by the nematocysts of the genus Nematoda, in a large breeding container having a ventilation hole, and curing the nematodes for a predetermined period of time.

In this case, if the aphid which is prey of the nematode larvae is a bivalve aphid, a bivalve aphid, etc., the parasitic parasitic plant which is proliferated can use plants and plants such as barley and wheat, and when the aphid is aphid, aphid, cotton aphid, , And branches, but is not limited to these.

An artificial diet containing 30 weight percent sugar, 3 weight percent yeast, 3 weight percent honey, and 3 weight percent pollen may be used as the composition of the adult artificial diet.

The eggs can be recovered conveniently by collecting the hereditary spots and collecting the nematode grasshopper eggs after the adult eggs are scattered by covering the eggs above the vessel.

For example, a cylindrical container having a diameter of about 10 cm and a height of about 5 to 10 cm is suitable, and a container having a mesh or the like attached to the wall of the container or the top of the container for ventilation is used.

Meanwhile, when the insect parasitic plant is barley, the step 200 of seeding the barley 200 lips into a flower pot having a diameter of 20 cm, and then germinating the seedlings at a rate of 1 to 2 grains per week And on the second day after the inoculation, a pollen inoculated with aphids is placed in a square cage of about 50 cm × 50 cm × 50 cm (width × length × height), and inoculated with about 100 genus grasshopper eggs.

In addition, the number of nematode grasshopper eggs to be inoculated is calculated on the basis of the number of feeding aphids in the breeding cage, and suitably between 10: 1 and 5: 1 for the number of nematode eggs for feeding aphids.

After 7 days, water is supplied to the barley, and the water supply is stopped from the 7th day to inhibit the growth of parasitic aphids. The cage is kept in the incubation period for about 20 days to 40 days, 33 ℃) or humidity (60 ~ 90% relative humidity), about 50% or more of the inoculated individuals become adults. When the adult begins to grow, the adult can be recovered for about 7 days, packed in the egg state after curing according to the product standard, or stored for about a week in a dark room at about 4 ° C, and reused for breeding.

Breeding containers (21 × 15 cm, height 10 cm suitable) have at least air circulation with ventilation holes with a mesh-like net so that they have fine grooves for ventilation or that insects do not come out. Use the container and put about 150 pieces of nematode grasshopper eggs in the breeding container, put 1g of eggs in the moth, and wrap them in the product container for about 2 days after the hatching for about 3 days.

Hereafter, the process of biological control of insect pests (including insect worms) by indigenous natural enemies (including nematode) and the process of detecting the optimal breeding conditions by examining the development conditions of indigenous enemies are shown in the attached tables and drawings The following will be described in detail.

<Test 1> Ecological investigation of pest and native nematode

FIG. 1 is a table for examining 7 kinds of indigenous nematode occurrences including 9 insect pests and nematode nematodes, such as powdery moth pest insects, occurring in the domestic game area. Fig. 2 is a graph And FIG. 3 is a chart showing seven species including domestic nemesis and nematode rootstock in the domestic game area.

end. Targeted enemy (insect): predatory grasshopper (interspecific insect)

I. Time of survey: Late March - Late October

All. Surveyed area: Mars, Anseong, Gapyeong, etc.

la. Survey method: direct survey (adult water / 3 weeks) and collection

hemp. Survey content: pest species and damage degree, food species spectrum and industrial potential

In the "Test 1" as shown in FIGS. 1 to 3, broad-nosed nematodes with a feeding spectrum for insect pests such as aphids and insects were examined as promising industrial insects.

&Lt; Test 2 >

FIG. 4 is a functional test chart for measuring changes in food intake according to the food density of the larvae of the nematode Nematoda, and FIG. 5 is a functional test chart for measuring the developmental characteristics and predatory power of the nematode, The optimal growth temperature was 20 ℃ and the light condition was 16 hours. It was found that the larvae predationed more than 440 insect pests during larval period (about 25 days).

end. Processing temperature: 10 ° C, 15 ° C, 20 ° C, 25 ° C, 30 ° C, 35 ° C

I. Content of research: Developmental characteristics, feeding ability, etc.

<Test 3> Spectral survey

FIG. 6 is a chart of the nematode fauna feeding spectrophotometer measuring the feeding ability of the nematode (nematode) of the nematode nematode. Fig. 7 shows the photograph of the nematode of the nematode, and the right side shows the nematode of the nematode It is a photograph taken.

Fig. 8 is a chart of the predation ability of the nematodes of the genus Apertivorus (second instar). Fig. 9 shows photographs of the aphid photographs on the left side, photographs of the flower pear trees on the center and photographs of the nematode larvae FIG. 10 is a chart of the feeding response (2013) of cotton aphids of the genus Aesculapia larvae (second instar larvae) in 2013, wherein the total number of birds per day for the second- , And it was increased with more food.

end. Target pests: little pear, aphids, etc.

I. Survey content: Pest insect species, insect pest eating power

<Test 4> Selection of low-toxicity drugs against enemies

Fig. 11 is a chart for examining the insecticide susceptibility of nematode nematodes, and three species such as acetaminopyride wettable powder as a low-toxicity insecticide for the nematodes have been selected.

end. Test materials: pears Registered pesticides and disinfectants

I. Survey content: Insecticide rate of natural enemies

<Test 5> Verification test of the genetic nematode (2013)

Table 12a is a chart comparing the change in the density of the insecticidal insect pests of the genus Nematoda, and Table 12b is a chart comparing the changes in the insecticidal density of the insecticidal insects of the genus Nematoda (summer) FIG. 13 is a photograph showing the effect of controlling the insect control of starchy mildew, FIG. 13 is a photograph of the top and the photograph of the left side, and FIG. 14 is a photograph of the mosquito bug worm, 15 is a chart comparing the report quality of the nematode,

As a result of the inoculation test, the effect of controlling the insecticide resistance was 43.0 ~ 64.5% after the 2nd inoculation of the nematode rootstock, and 82.8 ~ 89.0% , Respectively.

In addition, the weight of pear blight due to the nematode treatment was increased to 614 ~ 620g compared to the untreated 449g. Especially, the sugar content (.Bx) decreased significantly compared to the untreated 11.2fh treatment (12.6 ~ 12.8) Respectively.

end. Test site (varieties): Anseong-si Miyang-myeon Singi-ri 45 Kim Sang-eul farmhouse

I. Target insect: Pear insect insect

All. Tested enemies:

Claims (11)

A step of emitting nematode grasshopper eggs to a plant in a breeding cage in which food aphids are artificially proliferated;
Maintaining the temperature and the humidity in the breeding cage at a constant condition for 20 to 40 days so that about 50% or more of the radiated nematode eggs are fished with adults;
Collecting a pair of nematocysts of the genus Nematoda with male adults, and transferring them to a packed vessel;
Periodically selectively supplying any one of a sponge or a substitute food impregnated with an adult artificial diet to the container vessel;
The method according to claim 1, wherein the nematode budworm eggs scattered by the nematode budgerigar are collected from a container and placed in a large rearing container having a ventilation hole for curing for a predetermined period of time. 2. The method according to claim 1, wherein the ratio of the nematode budworm eggs is 10: 1 to 5: 1 according to the number of the feeding aphids that are artificially proliferated in the plants in the breeding cage. . The method according to claim 1, wherein the temperature in the breeding cage is maintained at 18 to 33 캜 and the humidity is maintained at 60 to 90%. [2] The method according to claim 1, wherein the packed vessel has a height of 5 to 10 cm and a diameter of 10 cm, and a net is formed on the wall or upper part so as to allow ventilation. The method of claim 1, wherein the composition of the adult artificial diet comprises 30 weight percent sugar, 3 weight percent yeast, 3 weight percent honey, and 3 weight percent pollen based on the total weight. How to breed the nemesis. The method according to claim 1, wherein the adult moth larva is an adult moth larva. [2] The method according to claim 1, wherein the cap is covered with an oil field above the container, and after the adult is scattered, the herbarium is collected so as to simultaneously recover the nematode grasshopper eggs. [3] The method according to claim 1, wherein the large breeding container has a width of 21 cm, a length of 15 cm, and a height of 20 cm. The method according to claim 1, wherein the step of inoculating the insect parasitic plant with the nematode grasshopper is performed by inoculating 1 to 2 aphids per week when the barley is sown in the pot, Wherein the inoculated flowerpot is inoculated with a nematode grasshopper egg. The method according to claim 9, further comprising the step of supplying moisture to the barley for a predetermined period of time after the seedling seedling is inoculated. The method according to claim 10, further comprising the step of stopping the supply of water for a predetermined period of time before collecting the emergent adult nematode and transferring the nematodes to a picking container to inhibit the growth of parasitic aphids A method of nematode budgerigar rearing for controlling insect pests.

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