CN107821339A - A kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application - Google Patents
A kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application Download PDFInfo
- Publication number
- CN107821339A CN107821339A CN201711317735.7A CN201711317735A CN107821339A CN 107821339 A CN107821339 A CN 107821339A CN 201711317735 A CN201711317735 A CN 201711317735A CN 107821339 A CN107821339 A CN 107821339A
- Authority
- CN
- China
- Prior art keywords
- zanjon
- monochamus alternatus
- alternatus hope
- cocoon honeybee
- cocoon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001442207 Monochamus alternatus Species 0.000 title claims abstract description 61
- 241000256844 Apis mellifera Species 0.000 title claims abstract description 54
- 238000009395 breeding Methods 0.000 title claims abstract description 15
- 241001481710 Cerambycidae Species 0.000 claims abstract description 18
- 239000000835 fiber Substances 0.000 claims abstract description 14
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 13
- 235000011613 Pinus brutia Nutrition 0.000 claims description 13
- 241000018646 Pinus brutia Species 0.000 claims description 13
- 238000005520 cutting process Methods 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 230000008901 benefit Effects 0.000 claims description 3
- 235000012907 honey Nutrition 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 235000010204 pine bark Nutrition 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 2
- 241000027558 Thyestilla gebleri Species 0.000 abstract description 2
- 230000003071 parasitic effect Effects 0.000 description 11
- 241000164724 Dastarcus helophoroides Species 0.000 description 3
- 241000885647 Paranthrene tabaniformis Species 0.000 description 3
- 241000219000 Populus Species 0.000 description 3
- 241000254173 Coleoptera Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 206010039710 Scleroderma Diseases 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 241000256837 Apidae Species 0.000 description 1
- 241000243771 Bursaphelenchus xylophilus Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 206010062701 Nematodiasis Diseases 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000467911 Sclerodermus guani Species 0.000 description 1
- 241000281311 Sclerodermus sichuanensis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Present invention is disclosed a kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application, by select the perpendicular hair longicorn of fiber crops (Thyestilla gebleri)Hosts of the larva as Monochamus alternatus Hope zanjon cocoon honeybee, while condition is bred in regulation and control, and selection suitably breeds condition, successfully realizes artificial propagation in scale Monochamus alternatus Hope zanjon cocoon honeybee, and applied to preventing and treating Monochamus alternatus Hope.
Description
Technical field
The present invention relates to a kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application, belong to biological control of insect pests
Technical field, more particularly to a kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee (Bracomorpha sp.) and pine it is brown
Application of the longicorn zanjon cocoon honeybee in Monochamus alternatus Hope is prevented and treated.
Background technology
The pine nematode propagated by Monochamus alternatus Hope (Monochamus alternatus Hope)
(Bursaphelenchus xylophilus Steiner et Buhrer) is China's pest and disease damage the most serious, at present
Henan, Shaanxi and the Shandong Province of other 13 provinces and the north of the south China in addition to Hainan Province are traveled to, it is annual lethal a large amount of
Pine tree, cause serious economy and Ecological Loss.It is to continue prevention and control pine using natural enemy insect biological control Monochamus alternatus Hope
One of effective measures of nematodiasis.At present, the mainly swollen leg honeybee of the natural enemy of widely used Monochamus alternatus Hope and dastarcus helophoroides
(Dastarcus helophoroides Fairmaire), wherein swollen leg honeybee has Scleroderma Xiao et Wu (Sclerodermus guani
Xiao et Wu), Scleroderma sichuanensis Xiao (S.sichuanensis Xiao) and Monochamus alternatus Hope swell three kinds of leg honeybee (S.sp.) should
Use in production.In woodland, the 1-3 age low instar larvaes for the leg honeybee main parasitic Monochamus alternatus Hope that swells, and dastarcus helophoroides main parasitic is old
Instar larvae, pupa and a small number of adults not yet to portal that just sprout wings.For the larva in 4-5 ages, the parasitic rate of both natural enemies is relatively low.
2013 in zunyi, guizhou, it has been found that Important Natural Enemy --- the Monochamus alternatus Hope zanjon cocoon of mature larvae in parasitic Monochamus alternatus Hope
Honeybee (Bracomorpha sp.), it is up to 30% in some areas nature parasitic rate, in 1 year two generation of Zunyi Prefecture, generation weight
It is folded, have a good application prospect, and a Monochamus alternatus Hope larva at most can parasitic 11 Monochamus alternatus Hope zanjon cocoon honeybees.Separately
Outside, no matter being a parasitic Monochamus alternatus Hope zanjon cocoon honeybee on Monochamus alternatus Hope body, or bull, finally Monochamus alternatus Hope can be killed
Extremely.
Based on the above-mentioned advantage of Monochamus alternatus Hope zanjon cocoon honeybee (Bracomorpha sp.), our team combine conventional warp
Test, by being continually striving to, the larva for erecting hair longicorn (Thyestilla gebleri) by the use of fiber crops is covered as Hosts with bark
Lid method artificial breeding Monochamus alternatus Hope zanjon cocoon honeybee, and it is applied to Stand control Monochamus alternatus Hope.
The content of the invention
It is an object of the invention to provide a kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application, people is realized
Work is bred Monochamus alternatus Hope zanjon cocoon honeybee and stored, control and application.
To achieve the above object, the present invention proposes following technical scheme：
A kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee, it is characterised in that comprise the following steps：(1) selection is replaced
Master is sent, (two) artificial breeding, (three) low-temperature storage；
Preferably, described selection Hosts comprise the following steps:The larva of the perpendicular hair longicorn of selection fiber crops is posted as replacement
It is main；
Preferably, described artificial breeding comprises the following steps:
(1) first by pine juggle cutting, the size of the groove is long 3cm, wide 1cm, deep 0.3cm；By the perpendicular hair longicorn of fiber crops
In larva access pine cutting, and fixed after being covered with pine bark with sealed membrane, obtain juggle A；
(2) juggle A is inserted into the having in the plastic cylinder of bottom of long 20cm diameters 5cm, then access health and through hypergamasis
Monochamus alternatus Hope zanjon cocoon honeybee queen bee, is sealed plastic cylinder with gauze and rubber band, and an every 3 days honey of feeding and water, every 5 days more
Change a juggle A, the quantity ratio of the Monochamus alternatus Hope zanjon cocoon honeybee queen bee and the thyestillageblerifaclarvaann larvaann is 1:1；
(3) the juggle A changed in step (2) is placed on 21 DEG C -22 DEG C of temperature, the artificial climate of relative humidity 50%
In case, illumination condition is nature dim light, is cultivated 1-2 days；It it is again 27 DEG C -28 DEG C by the temperature adjustment in growth cabinet, relatively
Humidity 70%, cultivate 10-11 days, cocoond to Monochamus alternatus Hope zanjon cocoon honeybee；
Preferably, described low-temperature storage comprises the following steps:
The cocoon of the Monochamus alternatus Hope zanjon cocoon honeybee is taken out from pine cutting, is subsequently placed in long 5cm diameters 1cm glass
Guan Zhong, blocked up with tampon, be placed in temperature as 10 DEG C -12 DEG C, relative humidity is to be stored in 40-50% growth cabinet；
Present invention further teaches application of the Monochamus alternatus Hope zanjon cocoon honeybee in Monochamus alternatus Hope is prevented and treated, in the 3-5 of Monochamus alternatus Hope
The instar larvae phase, temperature will be stored in as 10 DEG C -12 DEG C, Monochamus alternatus Hope zanjon in the growth cabinet that relative humidity is 40-50%
The cocoon of cocoon honeybee takes out, and is transported to woodland, selects fine day of the temperature higher than 20 DEG C or cloudy day release, is placed in glass tube during release
On the trunk of aggrieved plant, the mouth of pipe is down；
Preferably, the Monochamus alternatus Hope zanjon cocoon honeybee does harm to ratio 3 in the burst size of woodland according to benefit:1 release.
The beneficial effects of the invention are as follows：
1. using Hosts of the larva of the perpendicular hair longicorn of fiber crops as Monochamus alternatus Hope zanjon cocoon honeybee, while bar is bred in regulation and control
Part, Monochamus alternatus Hope zanjon cocoon honeybee are obviously improved to the parasitic rate of host.
2. the present invention can be advised manually using Hosts of the larva of the perpendicular hair longicorn of fiber crops as Monochamus alternatus Hope zanjon cocoon honeybee
Modelling breeds Monochamus alternatus Hope zanjon cocoon honeybee, has excellent popularizing application prospect.
Embodiment
Clear, complete description is carried out to the technical scheme of the embodiment of the present invention below：
Embodiment 1
1st, test method：(experiment is in triplicate)
(1) first by pine juggle cutting, the size of the groove is long 3cm, wide 1cm, deep 0.3cm, prepares 15 sections of pines, often
The upper cuttings of Duan Songmu 3；The mature larva of the perpendicular hair longicorn of fiber crops is accessed in pine juggle cutting, and covered with pine bark, is used
Parafilm sealed membranes are fixed, and obtain juggle A；
(2) the having in the plastic cylinder of bottom of long 20cm diameters 5cm and then by juggle A is put into, then access health and by handing over
The Monochamus alternatus Hope zanjon cocoon honeybee queen bee matched somebody with somebody, sealed, an every 3 days honey of feeding and water, changed once within every 5 days with gauze and rubber band
Juggle A, the Monochamus alternatus Hope zanjon cocoon honeybee queen bee and the quantity ratio of the perpendicular hair longicorn mature larva of the fiber crops are 1:1；
(3) the juggle A changed is placed on 21 DEG C -22 DEG C of temperature, in the growth cabinet of relative humidity 50%, illumination
Condition is nature dim light, cultivate 1-2 days, then by the temperature adjustment in growth cabinet be 27 DEG C -28 DEG C, relative humidity 70%,
Culture 10 days；Cocoond to Monochamus alternatus Hope zanjon cocoon honeybee；Period, situation was bred in observation daily, counted parasitic rate；
(4) parallel control is set, and the mature larva of the perpendicular hair longicorn of fiber crops is substituted for into the spot buprestid beetle larva of poplar ten, Li Shan respectively
Roundheaded borer, paranthrene tabaniformis larva, then repeat the above steps (1), (2), (3) respectively, obtains 1 (the spot Ji fourth of poplar ten of control
Worm larva), 2 (mountain longicorn mature larvas) of control, 3 (paranthrene tabaniformis larvas) of control.
The effect that 1 different Hosts of table breed Monochamus alternatus Hope zanjon cocoon honeybee compares
| Host | Parasitic rate (%) |
| The present invention (the perpendicular hair longicorn mature larva of fiber crops) | 85.35 |
| Compare 1 (the spot buprestid beetle larva of poplar ten) | 12.27 |
| Compare 2 (mountain longicorn mature larvas) | 63.18 |
| Compare 3 (paranthrene tabaniformis larvas) | Breed unsuccessfully |
The present invention uses the perpendicular hair longicorn mature larva of fiber crops to breed Monochamus alternatus Hope zanjon cocoon honeybee, Monochamus alternatus Hope as Hosts
Parasitic rate of the zanjon cocoon honeybee on host, which is significantly higher than, to be compareed.The larva for showing the perpendicular hair longicorn of fiber crops is excellent Hosts.
Claims (6)
1. a kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee, it is characterised in that comprise the following steps：（One）Selection substitutes
Host,（Two）Artificial breeding,（Three）Low-temperature storage.
2. the artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee according to claim 1, it is characterised in that described selection
Hosts comprise the following steps:The larva of the perpendicular hair longicorn of selection fiber crops is as Hosts.
3. the artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee according to claim 2, it is characterised in that described is artificial
Breed and comprise the following steps:
（1）First by pine juggle cutting, the size of the groove is long 3cm, wide 1cm, deep 0.3cm；By the larva of the perpendicular hair longicorn of fiber crops
Access in pine cutting, and fixed after being covered with pine bark with sealed membrane, obtain juggle A；
（2）Juggle A is inserted into the having in the plastic cylinder of bottom of long 20cm diameters 5cm, then access health and loose brown through hypergamasis
Longicorn zanjon cocoon honeybee queen bee, is sealed plastic cylinder with gauze and rubber band, an every 3 days honey of feeding and water, changes one within every 5 days
The quantity ratio of secondary juggle A, the Monochamus alternatus Hope zanjon cocoon honeybee queen bee and the thyestillageblerifaclarvaann larvaann is 1:1,
（3）By step（2）In the juggle A that changes be placed on 21 DEG C -22 DEG C of temperature, in the growth cabinet of relative humidity 50%,
Illumination condition is nature dim light, is cultivated 1-2 days；It is again 27 DEG C -28 DEG C by the temperature adjustment in growth cabinet, relative humidity
70%, cultivate 10-11 days, cocoond to Monochamus alternatus Hope zanjon cocoon honeybee.
4. the artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee according to claim 3, it is characterised in that described low temperature
Storage comprises the following steps:The cocoon of the Monochamus alternatus Hope zanjon cocoon honeybee is taken out from pine cutting, it is straight to be subsequently placed in long 5cm
In footpath 1cm glass tube, blocked up with tampon, be placed in temperature as 10 DEG C -12 DEG C, relative humidity is in 40-50% growth cabinet
Stored.
5. application of the Monochamus alternatus Hope zanjon cocoon honeybee in Monochamus alternatus Hope is prevented and treated described in claim 1-4 any one, its feature
It is, in the 3-5 instar larvae phases of Monochamus alternatus Hope, temperature will be stored in as 10 DEG C -12 DEG C, relative humidity is 40-50% artificial gas
The cocoon for waiting Monochamus alternatus Hope zanjon cocoon honeybee in case takes out, and is transported to woodland, selects fine day of the temperature higher than 20 DEG C or cloudy day release,
Glass tube is placed on the trunk of aggrieved plant during release, the mouth of pipe is down.
6. application of the Monochamus alternatus Hope zanjon cocoon honeybee according to claim 5 in Monochamus alternatus Hope is prevented and treated, it is characterised in that institute
Monochamus alternatus Hope zanjon cocoon honeybee is stated in the burst size of woodland according to benefit evil ratio 3:1 release.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711317735.7A CN107821339A (en) | 2017-12-12 | 2017-12-12 | A kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711317735.7A CN107821339A (en) | 2017-12-12 | 2017-12-12 | A kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107821339A true CN107821339A (en) | 2018-03-23 |
Family
ID=61643038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711317735.7A Pending CN107821339A (en) | 2017-12-12 | 2017-12-12 | A kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107821339A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771819A (en) * | 2020-07-09 | 2020-10-16 | 武汉市园林科学研究院 | Application of galleria mellonella larva as intermediate host in artificial breeding of dastarcus helophoroides |
| CN112385611A (en) * | 2020-11-20 | 2021-02-23 | 中国林业科学研究院森林生态环境与保护研究所 | Breeding method of Chouioia cunea Yang and application of breeding method in preventing and treating Chouioia cunea Yang |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04202104A (en) * | 1990-11-29 | 1992-07-22 | Norin Suisansyo Sanshi Konchiyuu Nogyo Gijutsu Kenkyusho | Method for controlling long-horned beetles using culture of natural enemy mold |
| CN103355261A (en) * | 2013-07-30 | 2013-10-23 | 天津拓华生物科技有限责任公司 | Application of thyestillageblerifacldermann larva as host to artificial propagation of scleroderma guani |
| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
-
2017
- 2017-12-12 CN CN201711317735.7A patent/CN107821339A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04202104A (en) * | 1990-11-29 | 1992-07-22 | Norin Suisansyo Sanshi Konchiyuu Nogyo Gijutsu Kenkyusho | Method for controlling long-horned beetles using culture of natural enemy mold |
| CN103355261A (en) * | 2013-07-30 | 2013-10-23 | 天津拓华生物科技有限责任公司 | Application of thyestillageblerifacldermann larva as host to artificial propagation of scleroderma guani |
| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
Non-Patent Citations (2)
| Title |
|---|
| 杨远亮: ""利用天敌昆虫防治松褐天牛技术研究"", 《中国优秀博士学位论文全文数据库农业科技辑》 * |
| 高尚坤: ""利用生命表技术评价生物防治松褐天牛控制松材线虫病的效果研究"", 《中国优秀博士学位论文全文数据库农业科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771819A (en) * | 2020-07-09 | 2020-10-16 | 武汉市园林科学研究院 | Application of galleria mellonella larva as intermediate host in artificial breeding of dastarcus helophoroides |
| CN112385611A (en) * | 2020-11-20 | 2021-02-23 | 中国林业科学研究院森林生态环境与保护研究所 | Breeding method of Chouioia cunea Yang and application of breeding method in preventing and treating Chouioia cunea Yang |
| CN112385611B (en) * | 2020-11-20 | 2022-08-09 | 中国林业科学研究院森林生态环境与自然保护研究所 | Breeding method of Chouioia cunea Yang and application of breeding method in preventing and treating Chouioia cunea Yang |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101589697B (en) | Captive-breed piebald dastarcus helophoroides and method applied to prevent and control monochamus alternatus hope | |
| CN104996362B (en) | A kind of swollen leg honeybee of artificial breeding Chinese wax Ji fourth and the method applied to preventing and treating trunk borer | |
| CN103262827A (en) | Method for feeding tea green leafhoppers indoors | |
| CN101574075A (en) | Artificial propagation technology of mallambyx raddei dastarcus helophoroides and technology for applying dastarcus helophoroides for controlling mallambyx raddei | |
| CN103004704A (en) | Method for breeding Scleroderma guani Xiao et Wu by using barley pests as intermediate hosts | |
| CN105028333A (en) | Method for artificially and largely raising Dioryctria pryeri indoors | |
| CN107980729A (en) | A kind of interior breeding mealybug and the method for Ban Shi encyrtids | |
| Mattson Jr | Cone resources and the ecology of the red pine cone beetle, Conophthorus resinosae (Coleoptera: Scolytidae) | |
| CN107821339A (en) | A kind of artificial breeding method of Monochamus alternatus Hope zanjon cocoon honeybee and its application | |
| CN103355261A (en) | Application of thyestillageblerifacldermann larva as host to artificial propagation of scleroderma guani | |
| CN103766324A (en) | Method for biologically preventing green plant bugs by peristenus spretus in winter jujube orchard | |
| Gur et al. | The Influence of Root Temperature on Apple Trees II. Clonal Differences in Susceptibility to Damage Caused by Supraoptimal Root Temperature | |
| CN107787926A (en) | A kind of artificial breeding method of the swollen leg honeybee of apple little Ji fourths and the application in the small buprestid beetle of apple is prevented and treated | |
| CN107114326B (en) | Artificial breeding method of Monochamus alternatus Hope and application of method in prevention and treatment of trunk-boring pests in forest trees | |
| CN100998291B (en) | Method for breeding tomato of preventing root-knot nematode disease | |
| Fitriana et al. | Effect of combination explant difference leaf part and concentration of active charcoal on callus initiation mangrove (Rhizophora apiculata BI) by in-vitro | |
| CN101803586B (en) | Scale artificial culture technology of papilio xuthus Linnaeus in northern areas | |
| Adamec | Dark respiration of leaves and traps of terrestrial carnivorous plants: are there greater energetic costs in traps? | |
| CN114651793A (en) | Artificial breeding of trichogramma obscura and method for preventing and treating spodoptera frugiperda by using trichogramma obscura | |
| KR101153456B1 (en) | Rearing method of lycaena dispar in a room condition | |
| CN103999680B (en) | Plasma treatment willow branch produces the method for bud mutation | |
| CN102389066A (en) | Preparation method of chilo suppressalis artificial feed | |
| EP4003021A1 (en) | Feed stuff for beneficial organisms that can be used in integrated pest management | |
| Hsiao et al. | Effects of flooding on rooting and sprouting of isolated stem segments and on plant growth of Paspalum distichum L. | |
| Vidyasagaran et al. | Germination and emergence of four rattan Calamus species of Western ghats in response to different pre-sowing seed treatments |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180323 |
|
| RJ01 | Rejection of invention patent application after publication |