CN104996362B - A kind of swollen leg honeybee of artificial breeding Chinese wax Ji fourth and the method applied to preventing and treating trunk borer - Google Patents

A kind of swollen leg honeybee of artificial breeding Chinese wax Ji fourth and the method applied to preventing and treating trunk borer Download PDF

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Publication number
CN104996362B
CN104996362B CN201510359064.5A CN201510359064A CN104996362B CN 104996362 B CN104996362 B CN 104996362B CN 201510359064 A CN201510359064 A CN 201510359064A CN 104996362 B CN104996362 B CN 104996362B
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chinese wax
honeybee
leg honeybee
swollen leg
swollen
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CN104996362A (en
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唐艳龙
张彦龙
王小艺
杨忠岐
魏可
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Research Institute of Forest Ecology Environment and Protection of Chinese Academy of Forestry
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Research Institute of Forest Ecology Environment and Protection of Chinese Academy of Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M99/00Subject matter not provided for in other groups of this subclass

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Wood Science & Technology (AREA)
  • Insects & Arthropods (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Feed For Specific Animals (AREA)

Abstract

Present invention is disclosed a kind of artificial breeding method of the swollen leg honeybee of Chinese wax Ji fourth and the application in forest trunk borer is prevented and treated, by selecting small peak Xiong Feng (Bombus hypocrita) drone pupae to be used as the Hosts of the swollen leg honeybee of Chinese wax Ji fourth, regulate and control Reproduction Conditions simultaneously so that the parasitic ability for the swollen leg honeybee of Chinese wax Ji fourth for breeding to obtain is significantly improved;It is good to the parasitic effects of 13 age mountain longicorn larvas using the mountain longicorn of the swollen leg honeybee preventing and treating the Northeast of Chinese wax Ji fourth, the swollen leg honeybee of indoor Chinese wax Ji fourth.

Description

A kind of swollen leg honeybee of artificial breeding Chinese wax Ji fourth and the method applied to preventing and treating trunk borer
Technical field
The present invention relates to biological control of insect pests technical field, more particularly to a kind of swollen leg honeybee of artificial breeding Chinese wax Ji fourth The artificial breeding method of (Sclerodermus pupariae Yang et Yao) and Chinese wax Ji fourth swell leg honeybee in preventing and treating forest Application in trunk borer.
Background technology
The preventing and treating of trunk borer is always global problem.Such as carry pine nematode (Bursaphelenchus Xylophilus Monochamus alternatus Hope (Monochamus alternatus)), causes a large amount of pine tree withered deaths in China.Endanger oak The mountain longicorn (Massicus raddei) of tree, cause a large amount of roburs in the Northeast of China dead.Endanger the apple little Ji of apple tree Tintinnid (Agrilus mali) carrys out destructive disaster to the wilding forest belt in Xinjiang of China area.And anoplophora glabripennis After (Anoplophora glabripennis) and Emerald ash borer (Agrilus planipennis) are passed to the U.S., turn into beautiful The forest pest of state's most serious, cause a large amount of tree deaths.Therefore, how to preserve the ecological environment and protect bio-diversity Under the premise of effective control trunk borer harm, the characteristics of turning into current research both at home and abroad one of problem.Given birth to using natural enemy insect Thing preventing and treating trunk borer is considered as one of most effective and sustainable prophylactico-therapeutic measures.At present, for preventing and treating longicorn class and Ji Ding Class trunk borer, most widely used natural enemy insect are dastarcus helophoroides (Dastarcus helophoroides) and swollen leg honeybee, its In swell leg honeybee class natural enemy mainly include Scleroderma Xiao et Wu (Sclerodermus guani) and Scleroderma sichuanensis Xiao (Sclerodermus sichuanensis)。
The swollen leg honeybee of Chinese wax Ji fourth belongs to Hymenoptera together with wide variety of Scleroderma Xiao et Wu and Scleroderma sichuanensis Xiao (Hymenoptera) Bethylidae (Bethylidae) sclerderm Bethylid (Sclerodermus), it is posting for discovered in recent years The parasitic wasp novel species of Emerald ash borer is born in, the honeybee female has higher wing rate, search host and attacking ability very strong, is a kind of Very excellent trunk borer natural enemy.Through research, it can also parasitic mountain longicorn, Monochamus alternatus Hope, anoplophora glabripennis, apple little Ji fourths A variety of forest trunk borers such as worm and the spot Ji fourth of poplar ten (Melanophila picta Pallas).
Can winter low temperature be to influence colonize successful key factor in release from south toward moving northward the natural enemy grown, therefore When natural enemy is grown in shifting, selection distribution is closer to the species for moving into ground as far as possible, to reduce injury of the winter low temperature to natural enemy as far as possible. The swollen leg honeybee of Chinese wax Ji fourth is found in Tianjin, (is found in than Scleroderma Xiao et Wu (being found in Shandong and Guangdong) and Scleroderma sichuanensis Xiao Sichuan) more by north, as little as -26.2 DEG C of its supercooling point, for cold districts such as preventing and treating mountain longicorn, agrilus malis Insect advantageously.At present, the apple of the mountain longicorn in northeast, the anoplophora glabripennis for preventing and treating Gansu and Xinjiang is prevented and treated using it Little Ji fourths achieve good effect, and Chinese wax Ji fourth swells leg honeybee in release area's safe overwintering, establishes stable population.
The content of the invention
It is an object of the invention to provide a kind of swollen leg honeybee (the Sclerodermus pupariae of artificial breeding Chinese wax Ji fourth Yang et Yao) method, and Chinese wax Ji fourth swells application of the leg honeybee in forest trunk borer is prevented and treated, with by using people The method that work is bred is bred the swollen leg bee imago of Chinese wax Ji fourth and stored, to be applied in preventing and treating forest trunk borer In.
To achieve the above object, the present invention proposes following technical scheme:A kind of artificial breeding side of the swollen leg honeybee of Chinese wax Ji fourth Method, it is characterised in that comprise the following steps:(1) Hosts are selected, (two) select honeybee kind, (three) artificial breeding;
Preferably, described selection Hosts comprise the following steps:Select small peak Xiong Feng (Bombus hypocrita) Drone pupae as Hosts;
Preferably, described selection honeybee kind comprises the following steps:Selection health and the swollen leg honeybee of Chinese wax Ji fourth through hypergamasis Queen bee is honeybee kind;
Preferably, described artificial breeding comprises the following steps:
(1) small peak Xiong Feng (Bombus hypocrita) drone pupae is put into dactylethrae, then access health and warp The Chinese wax Ji fourth of hypergamasis is swollen leg honeybee queen bee, and the finger-type mouth of pipe is stoppered with absorbent cotton, the Chinese wax Ji fourth swell leg honeybee queen bee with it is described The quantity ratio of small peak bear peak drone pupae is 1: 1-1: 2;
(2) dactylethrae is placed on 22.5 DEG C -23.5 DEG C, in the growth cabinet of relative humidity 55%, cultivated 12-13 days; It is again 27 DEG C by the temperature adjustment in growth cabinet, relative humidity 65%, cultivates 22-27 days, the swollen leg honeybee of Chinese wax Ji fourth starts Sprout wings, the swollen leg honeybee after emergence is taken out from growth cabinet, is then selected, is standby;
Preferably, it is 8-15 DEG C that the swollen leg bee imago of Chinese wax Ji fourth that the artificial breeding comes out, which is placed in temperature, humidity 30- Stored in 50% growth cabinet, and storage time is no more than 3 months;
Present invention further teaches swollen application of the leg honeybee in forest trunk borer is prevented and treated of Chinese wax Ji fourth, in forest trunk borer The low instar larvae phase, the Chinese wax Ji fourth of the storage leg bee imago that swells is taken out from microthermal climate case, woodland is transported to, selects temperature Fine day higher than 20 DEG C or cloudy day release, glass tube is placed in aggrieved plant bottoms or base portion during release, the mouth of pipe is down;
Preferably, Chinese wax Ji fourth swells, and the release of leg bee imago is preceding to investigate insect insect density, and randomly selects 30 pipe Chinese wax Ji The counting of the swollen leg honeybee of fourth, draws average;
Preferably, the Chinese wax Ji fourth swells burst size of the leg bee imago in woodland as according to beneficial harmful than 1: 1 release;
Preferably, in 30 days after Chinese wax Ji fourth swells the release of leg bee imago, by randomly selecting the aggrieved plant in part, solve Investigation is cutd open by the number of pest of parasitism to investigate parasitic rate;
Preferably, the forest trunk borer is mountain longicorn.
The beneficial effects of the invention are as follows:
1. using small peak Xiong Feng (Apis cerana cerana Fabricius) drone pupae as the swollen leg of Chinese wax Ji fourth The Hosts of honeybee, while regulate and control Reproduction Conditions so that the parasitic ability for the swollen leg honeybee of Chinese wax Ji fourth for breeding to obtain obtains significantly Lifting;Using the mountain longicorn of the swollen leg honeybee preventing and treating the Northeast of Chinese wax Ji fourth, indoor Chinese wax Ji fourth swells leg honeybee to Li Shan days 1-3 ages The parasitic effects of ox larva are good.
Embodiment
Clear, complete description is carried out to the technical scheme of the embodiment of the present invention below:
Embodiment 1
1st, test method:(experiment is in triplicate)
(1) small peak Xiong Feng (Bombus hypocrita) drone pupae is put into dactylethrae, then access health and warp The Chinese wax Ji fourth of hypergamasis is swollen leg honeybee queen bee, and the finger-type mouth of pipe is stoppered with absorbent cotton, the Chinese wax Ji fourth swell leg honeybee queen bee with it is described The quantity ratio of small peak bear peak drone pupae is 1: 2;
(2) dactylethrae is placed on 23 DEG C, in the growth cabinet of relative humidity 55%, cultivated 12 days;Again by artificial climate Temperature adjustment in case is 27 DEG C, relative humidity 65%, is cultivated 25 days, and the swollen leg honeybee of Chinese wax Ji fourth starts to sprout wings, after emergence Swollen leg honeybee is taken out from growth cabinet, is then selected, be standby.
(3) 15 30cm × 30cm × 20cm white plastic box is selected, 5 sections are put into every box and is connected to mountain longicorn children The robur juggle of worm (1-3 ages), the swollen leg honeybee of Chinese wax Ji fourth that breeding obtains in step (2) is put into plastic casing, Chinese wax Ji fourth swells The quantity ratio of leg honeybee and mountain longicorn larva is 1: 1;
(4) parallel control is set, and small peak Xiong Feng (Bombus hypocrita) drone pupae is substituted for into the spot of poplar ten respectively Buprestid beetle larva, paranthrene tabaniformis larva, then repeat the above steps (1), (2), (3) respectively, and obtaining control 1, (spot of poplar ten is lucky Tintinnid larva), compare 2 (paranthrene tabaniformis larvas);
(5) plastic casing after honeybee will be connect and be put into 25 DEG C, in the artificial incubator of relative humidity 75%, tree is splitted after 7-10 days Cortex counts the sneak case of the swollen leg honeybee of Chinese wax Ji fourth of different host's breedings.
Parasitic rate of the swollen leg honeybee of Chinese wax Ji fourth of 1 different hosts of table breeding to 1-3 age mountain longicorn larvas
The selection of host egg of the present invention to breed parasitism of the swollen leg honeybee of obtained Chinese wax Ji fourth to mountain longicorn larva Significant effect strengthens.
2nd, test method:(experiment is in triplicate)
(1) small peak Xiong Feng (Bombus hypocrita) drone pupae is put into dactylethrae, then access health and warp The Chinese wax Ji fourth of hypergamasis is swollen leg honeybee queen bee, and the finger-type mouth of pipe is stoppered with absorbent cotton, the Chinese wax Ji fourth swell leg honeybee queen bee with it is described The quantity ratio of small peak bear peak drone pupae is 1: 2;
(2) dactylethrae is placed on 23 DEG C, in the growth cabinet of relative humidity 55%, cultivated 12 days;Again by artificial climate Temperature adjustment in case is 27 DEG C, relative humidity 65%, is cultivated 25 days, and the swollen leg honeybee of Chinese wax Ji fourth starts to sprout wings, after emergence Swollen leg honeybee is taken out from growth cabinet, is then selected, be standby.
(3) 15 30cm × 30cm × 20cm white plastic box is selected, 5 sections are put into every box and is connected to mountain longicorn children The robur juggle of worm (1-3 ages), the swollen leg honeybee of Chinese wax Ji fourth that breeding obtains in step (2) is put into plastic casing, Chinese wax Ji fourth swells The quantity ratio of leg honeybee and mountain longicorn larva is 1: 1;
(4) parallel control is set, and the Reproduction Conditions in set-up procedure (2), is adjusted to 26 DEG C, relative humidity 60%, constant temperature and humidity breeding, other conditions are constant, obtain control 3;
Reproduction Conditions in set-up procedure (2), 25 DEG C being adjusted to, relative humidity 70%, constant temperature and humidity is bred, its His condition is constant, obtains control 4;
(5) plastic casing after honeybee will be connect and be put into 25 DEG C, in the artificial incubator of relative humidity 75%, tree is splitted after 7-10 days Cortex counts the sneak case of the swollen leg honeybee of Chinese wax Ji fourth of different host's breedings.
Parasitic rate of the swollen leg honeybee of the Chinese wax Ji fourth of the different reproductive condition of table 2 to 1-3 age mountain longicorn larvas
The selection of Reproduction Conditions of the present invention can significantly improve the Chinese wax Ji fourth that breeding obtains and swell leg honeybee to mountain longicorn children The parasitic effects of worm.

Claims (2)

  1. The artificial breeding method of leg honeybee 1. a kind of Chinese wax Ji fourth swells, it is characterised in that comprise the steps of:(1) selection substitutes Host, (two) select honeybee kind, (three) artificial breeding;Described selection Hosts comprise the following steps:Select Bombus hypocrita The drone pupae of (Bombus hypocrita) is as Hosts;Described selection honeybee kind comprises the following steps:Selection health and The swollen leg honeybee queen bee of Chinese wax Ji fourth through hypergamasis is honeybee kind;Described artificial breeding comprises the following steps:
    (1) drone pupae of Bombus hypocrita (Bombus hypocrita) is put into dactylethrae, then access is healthy and by handing over The swollen leg honeybee queen bee of Chinese wax Ji fourth matched somebody with somebody, the finger-type mouth of pipe, the swollen leg honeybee queen bee of the Chinese wax Ji fourth and the small peak are stoppered with absorbent cotton The quantity ratio of bumblebee drone pupae is 1:2;
    (2) dactylethrae is placed on 23 DEG C, in the growth cabinet of relative humidity 55%, cultivated 12-13 days;Again by growth cabinet In temperature adjustment be 27 DEG C, relative humidity 65%, cultivate 22-27 days, Chinese wax Ji fourth swell leg honeybee start to sprout wings, after emergence Swollen leg honeybee is taken out from growth cabinet, is then selected, be standby.
  2. The artificial breeding method of leg honeybee 2. Chinese wax Ji fourth according to claim 1 swells, it is characterised in that the artificial breeding The swollen leg bee imago of Chinese wax Ji fourth out is placed in temperature as 8-15 DEG C, and humidity is to be stored in 30-50% growth cabinet, And storage time is no more than 3 months.
CN201510359064.5A 2015-06-25 2015-06-25 A kind of swollen leg honeybee of artificial breeding Chinese wax Ji fourth and the method applied to preventing and treating trunk borer Expired - Fee Related CN104996362B (en)

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CN105918257A (en) * 2016-04-14 2016-09-07 伊犁哈萨克自治州林业科学研究院 Method for artificially breeding Sclerodermus pupariae by using local alternate hosts
CN106718395A (en) * 2016-12-01 2017-05-31 何振贤 It is a kind of to discharge the method that the swollen leg honeybee of Kazakhstan prevents and treats Chinese scholar tree control of Apriona swainsoni
CN106973866B (en) * 2017-05-17 2020-11-17 中国林业科学研究院森林生态环境与保护研究所 Artificial breeding method of eastern verruca plana and application of eastern verruca plana in prevention and control of forest pests
CN106962298A (en) * 2017-05-26 2017-07-21 何振贤 A kind of method of the grizzled bar longicorn in utilization Kazakhstan Zhong Tuifengfang Zhiduo County
CN112335613B (en) * 2020-11-20 2022-07-12 中国林业科学研究院森林生态环境与自然保护研究所 Breeding method of cerasus albolabris egg jumping wasps and application of breeding method in prevention and control of cerasus albolabris
CN112385611B (en) * 2020-11-20 2022-08-09 中国林业科学研究院森林生态环境与自然保护研究所 Breeding method of Chouioia cunea Yang and application of breeding method in preventing and treating Chouioia cunea Yang

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