CN1415196A - Method for breeding bethyloids by using yellow flour beetle as interhost - Google Patents

Abstract

A method for reproducing the Bethylidae, which can be used to prevent and elimiante the natural energy of tree (such as longican), features that the another insect "Huangfengjia" is used as its intermediate host.

Description

Utilize the bloom first to breed the method for swollen leg honeybee for vector

Technical field

The present invention relates to the biological control technical field, relate in particular to a kind of method of breeding swollen leg honeybee.

Background technology

At present, forest disease and pest has the trend of aggravation development in the world wide, and the forest loss that annual due to illness insect pest causes is very huge.Simultaneously, trunk borer such as longicorn is also very serious to the harm of gardens, trade trees in city trees and shrubs and scenic area.Utilize the natural enemy insect of these trunk borers---the trunk borer of economic crops such as swollen leg honeybee control forest, fruit tree, gardening and medicinal plant, because it has remarkable, the free of contamination characteristics of control efficiency, the more important thing is and can not produce because of using chemical pesticide to bring the problem of residue of pesticide for environment and agricultural product, and swollen leg honeybee controlling object is many, can discharge the area at it after the release and be perfunctory to diffusion, its prevention and treatment range is constantly enlarged, control efficiency heals and becomes obviously, thereby has obtained using comparatively widely.The large area control trunk borer needs the swollen leg honeybee of a large amount of commodity, therefore breeds the primary key that the swollen leg honeybee of commodity just becomes this control of insect technology in force.

Though swollen leg honeybee can utilize their natural host breeding, shortcoming is to obtain by artificial planing wood collection after natural host need be provided with trap-tree, and the collection difficulty is big.Therefore, if utilize the numerous honeybee of natural host, quantity of its numerous honeybee and time that can the numerous honeybee natural host situation occurred in the open air that places one's entire reliance upon can not realize that the numerous honeybee of batch production is to satisfy the needs of large area control.

Summary of the invention

The object of the present invention is to provide that a kind of to utilize cheap, the bloom first that conveniently is easy to get be the method that vector is in enormous quantities, swollen leg honeybee is bred in batch production.

To achieve these goals, the present invention has adopted following steps:

1. raise vector bloom first, (1) lays eggs and receive ovum: 1 hard slightly paper on the pad of the bottom of the case of laying eggs, put into artificial feed and bloom first adult then, add a cover raising, the density of adult is about 70～80/100cm2, when adult gave birth to the ovum of the overwhelming majority, the paper of will laying eggs earlier took out, and sifts out the worm excrement with mesh screen again, sift out the wheat bran of worm's ovum then, paper that is stained with ovum and wheat bran are put in the Breeding container, sprinkle the very thin feed of one deck, received ovum 1 time every 1～3 day, received behind the ovum 10～15 days, ovum is all hatched, and the paper that is stained with ovum is taken out, and newly hatched larvae is shaken off gently in Breeding container; (2) larva raising: every 2～3 days, every basin should add 250～500g feed after 8 ages, and the density of larva is controlled at 8～100,000/m2, during larvae development to 12～14 ages, with sieve feed, the worm excrement of larva with remnants separated, stay an amount of larva, allowing it continue to grow is adult; (3) collect after larval molting is pupated, under 4 ℃ of environment, deposited 24 hours;

2. breed swollen leg honeybee, 2～3 the bloom first pupas of at first in dactylethrae, packing into, insert 4～6 female swollen leg honeybees (worm honeybee ratio is 1: 2) of handing over tail with the writing brush brush then, with tampon mouth of pipe jam-pack is put into plastic crate again, every basket 250～300 pipe, labelled on basket, indicate and connect the honeybee date, worm honeybee ratio, bee hatching rate etc., on numerous honeybee frame, numerous honeybee indoor temperature is controlled at 28～30 ℃ with the basket pendulum, humidity 60～70%, make it lay eggs and grow to becoming honeybee, ovum is 13.60 ℃ to the threshold of development of sprouting wings, and effective accumulated temperature is 320.07 ℃, sooner or later open sterilamp half an hour every day, and the swollen leg honeybee parasitism of running check, spin, the emergence situation, the test tube that grows mould is in time chosen, treat that the filial generation honeybee all sprouted 3～5 days after, refrigerating chamber storage (temperature is controlled at 5～10 ℃) is put in the dactylethrae taking-up, and the commodity honeybee stores as ready then.

The larva of described vector bloom first generally makes feed with wheat bran, can also mix according to a certain percentage with wheat bran with manioc waste, soya bean slag, vinasse slag etc. and make feed.

Test shows, on average connects honeybee rate 70%～98%, and eclosion rate is more than 90%, and every vector bloom first larva can breed 10～18 of sub-honeybees.

The present invention can realize that industrialization breeds swollen leg honeybee and be used to prevent and treat trunk borers such as longicorn; help promoting the application of bio-control method in forest protection work; control spreading of time natural disposition insect, thereby reduce the use of chemical pesticide in a large number, to save human and material resources.Be applied in the insect aspect of economic crops such as control flowers, traditional Chinese medicine, fruit tree; will reduce operating cost; reduce the pollution of agricultural chemicals and residual, to the healthy of the production of " pollution-free food ", the protection people and safeguard that ecotope all has very positive meaning.

Embodiment

Below the present invention is further specified: 1. raise vector bloom first Tenebrio molitor L.

A. lay eggs and receive ovum: 1 hard slightly paper on the pad of the bottom of the case of laying eggs, put into artificial feed and bloom first adult then, add a cover raising, generally make feed, can also mix according to a certain percentage with wheat bran with manioc waste, soya bean slag, vinasse slag etc. and make feed with wheat bran.The density of adult is about 70～80/100cm2, and when adult gave birth to the ovum of the overwhelming majority, the paper of will laying eggs earlier took out, and sifts out the worm excrement with mesh screen again, sifts out the wheat bran of worm's ovum then, and paper that is stained with ovum and wheat bran are put in the Breeding container, sprinkles the very thin feed of one deck.Because adult is got the habit of the own ovum of food again, so received ovum 1 time, to receive behind the ovum 10～15 days every 1～3 day, ovum is all hatched, and the paper that is stained with ovum is taken out, and newly hatched larvae is shaken off gently in Breeding container;

B. larva raising: after 8 ages the growth of larva rapider, appetite increases severely.Every 2～3 days, every basin should add 250～500g feed, and the density of larva is controlled at 8～100,000/m2, during larvae development to 12～14 ages, with sieve larva and remaining feed, worm excrement separate, stayed an amount of larva, and allowing it continue growth is adult;

C. collect after larval molting being pupated, under 4 ℃ of environment, deposited 24 hours.2. breed the swollen leg honeybee Scleroderma guani Xiao et Wu of Guan Shi,

A. (2～3 the bloom first pupas of packing in 50 * 10mm) insert 4～6 female swollen leg honeybees (worm honeybee ratio is 1: 2) of handing over tail with the writing brush brush then at dactylethrae;

B. with tampon mouth of pipe jam-pack is put into plastic crate, every basket 250～300 pipe, labelled on basket, indicate and connect honeybee date, worm honeybee ratio, bee hatching rate etc.;

C. with basket pendulum on numerous honeybee frame, numerous honeybee indoor temperature is controlled at 28～30 ℃ (± 0.5 ℃), humidity 60～70% makes it lay eggs and grows to becoming honeybee, ovum is 13.60 ℃ to the threshold of development of emergence, effective accumulated temperature is 320.07 ℃;

D. open sterilamp half an hour every day sooner or later, and the swollen leg honeybee parasitism of running check, spin, the emergence situation, the test tube that grows mould is in time chosen;

E. after treating that the filial generation honeybee all sprouted 3～5 days, refrigerating chamber storage (temperature is controlled at 5～10 ℃) is put in the dactylethrae taking-up, the commodity honeybee stores as ready then.

Claims (2)

1. the method for utilizing the bloom first to breed swollen leg honeybee for vector is characterized in that and can realize by following steps:

(1) raises vector bloom first

A. lay eggs and receive ovum: 1 hard slightly paper on the pad of the bottom of the case of laying eggs, put into artificial feed and bloom first adult then, add a cover raising, the density of adult is about 70～80/100cm2, when adult gives birth to the ovum of the overwhelming majority, the paper of will laying eggs earlier takes out, sift out the worm excrement with mesh screen again, sift out the wheat bran of worm's ovum then, paper that is stained with ovum and wheat bran are put in the Breeding container, sprinkle the very thin feed of one deck, received ovum 1 time every 1～3 day, received behind the ovum 10～15 days, ovum is all hatched, the paper that is stained with ovum is taken out, newly hatched larvae is shaken off gently in Breeding container;

B. larva raising: every 2～3 days, every basin should add 250～500g feed after 8 ages, and the density of larva is controlled at 8～100,000/m2, during larvae development to 12～14 ages, with sieve feed, the worm excrement of larva with remnants separated, stay an amount of larva, allowing it continue to grow is adult;

C. collect after larval molting being pupated, under 4 ℃ of environment, deposited 24 hours;

(2) breed swollen leg honeybee

A. 2～3 the bloom first pupas of packing in dactylethrae insert 4～6 female swollen leg honeybees (worm honeybee ratio is 1: 2) of handing over tail with the writing brush brush then;

B. with tampon mouth of pipe jam-pack is put into plastic crate, every basket 250～300 pipe, labelled on basket, indicate and connect honeybee date, worm honeybee ratio, bee hatching rate etc.;

C. with basket pendulum on numerous honeybee frame, numerous honeybee indoor temperature is controlled at 28～30 ℃, humidity 60～70% makes it lay eggs and grows to becoming honeybee, ovum is 13.60 ℃ to the threshold of development of emergence, effective accumulated temperature is 320.07 ℃;

D. open sterilamp half an hour every day sooner or later, and the swollen leg honeybee parasitism of running check, spin, the emergence situation, the test tube that grows mould is in time chosen;

E. after treating that the filial generation honeybee all sprouted 3～5 days, refrigerating chamber storage (temperature is controlled at 5～10 ℃) is put in the dactylethrae taking-up, the commodity honeybee stores as ready then.

2. method of breeding swollen leg honeybee according to claim 1, it is characterized in that: the larva of described vector bloom first generally makes feed with wheat bran, can also mix according to a certain percentage with wheat bran with manioc waste, soya bean slag, vinasse slag etc. and make feed.