CN101589697A - Artificial propagation piebald dastarcus helophoroides and the technology that is applied to prevent and treat Monochamus alternatus Hope - Google Patents
Artificial propagation piebald dastarcus helophoroides and the technology that is applied to prevent and treat Monochamus alternatus Hope Download PDFInfo
- Publication number
- CN101589697A CN101589697A CN 200810114181 CN200810114181A CN101589697A CN 101589697 A CN101589697 A CN 101589697A CN 200810114181 CN200810114181 CN 200810114181 CN 200810114181 A CN200810114181 A CN 200810114181A CN 101589697 A CN101589697 A CN 101589697A
- Authority
- CN
- China
- Prior art keywords
- piebald
- dastarcus helophoroides
- adult
- ovum
- wooden unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The technology that the invention discloses a kind of artificial propagation piebald dastarcus helophoroides and be applied to prevent and treat Monochamus alternatus Hope, be that 25 ℃ ± 2 ℃, humidity are in 60% ± 5% the environment adult of piebald dastarcus helophoroides to be raised and bring out it is laid eggs in a large number at first, and hatch the larva of piebald dastarcus helophoroides in environmental temperature; Utilize alternative host to make its parasitism, growth then, turn into adult at last; The ovum or the adult of piebald dastarcus helophoroides can be made the standing forest that ovum release card or adult release bag or dactylethrae are released to the Monochamus alternatus Hope harm in pine nematode epidemic-stricken area afterwards.Utilize the natural enemy insect Pest Control, can free from environmental pollution, safeguarded the ecological balance and kept under the prerequisite of bio-diversity, insect and disease are obtained effectively and the control that continues is the advanced technology of control Monochamus alternatus Hope and pine nematode.
Description
Technical field
The present invention relates to a kind of biological control technology, the technology that relates in particular to a kind of artificial propagation piebald dastarcus helophoroides and be applied to prevent and treat Monochamus alternatus Hope.
Background technology
Monochamus alternatus Hope (Monochamus alternatus Hope) has another name called Monochamus alternatus, ponderous borer, belonging to coleoptera (Coleoptera) ditch shin longicorn subfamily (Laminae), is the particularly important trunk borer of masson pine (P.massoniana) of harm pine tree (Pinus spp.).The adult of Monochamus alternatus Hope gnaws the tender skin of the tender tip of pine tree during supplementing the nutrients, larva then eats into harm cambium and xylem in the trunk, cuts off the conducting tissue of trees, causes the host trees tree vigo(u)r weak, and weight person causes trees death.Monochamus alternatus Hope the more important thing is that except directly endangering the trees it can carry and propagate pine nematode (claiming pine tree wilt disease again).
The cause of disease of pine nematode is pine wood nematode (Bursaphelenchus xylophilus), is the great War Torn venereal disease evil of pine tree, becomes first of the forest disease and pest.At present, the common recognition and the control strategy of forestry circle are: at first must effectively control communication media---the Monochamus alternatus Hope of pine wood nematode, could control pine nematode.
The Monochamus alternatus Hope most of the time throughout one's life is a larval stage, gets food in trunk, and the disguised life of battalion in the prior art, mainly prevents and treats by chemical control and other conventional control methods.
There is following shortcoming at least in above-mentioned prior art:
Chemical control and other conventional control methods are difficult to effectively control Monochamus alternatus Hope, and contaminated environment.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can effectively prevent and treat Monochamus alternatus Hope, and artificial propagation piebald dastarcus helophoroides free from environmental pollution and the technology that is applied to prevent and treat Monochamus alternatus Hope.
The objective of the invention is to be achieved through the following technical solutions:
The technology of artificial propagation piebald dastarcus helophoroides of the present invention comprises step:
At first, the raising and bringing out of carrying out the adult of described piebald dastarcus helophoroides is laid eggs;
Then, carry out the hatching of ovum, hatch the first-instar young of described piebald dastarcus helophoroides;
Afterwards, utilize alternative host to raise the first-instar young of described piebald dastarcus helophoroides, make described first-instar young parasitize described alternative host and growth, sprouting wings at last the adult of described piebald dastarcus helophoroides, and described alternative host is larva or a kind of pupa of intending ground beetle of a kind of longicorn;
Environmental temperature in the said process is that 25 ℃ ± 2 ℃, humidity are 60% ± 5%.
The technology of control Monochamus alternatus Hope of the present invention is used the piebald dastarcus helophoroides control Monochamus alternatus Hope of the technology breeding of above-mentioned artificial propagation piebald dastarcus helophoroides, and the ovum or the adult of described piebald dastarcus helophoroides is released on the tree in described Monochamus alternatus Hope harm district.
As seen from the above technical solution provided by the invention, artificial propagation piebald dastarcus helophoroides of the present invention and the technology that is applied to prevent and treat Monochamus alternatus Hope, owing to be that 25 ℃ ± 2 ℃, humidity are in 60% ± 5% the environment adult of piebald dastarcus helophoroides to be raised and bring out to lay eggs at first, and hatch the larva of piebald dastarcus helophoroides in environmental temperature; Utilize alternative host to make it turn into adult then; Use above-mentioned piebald dastarcus helophoroides control Monochamus alternatus Hope afterwards, the epidemic-stricken area that the ovum or the adult of piebald dastarcus helophoroides can be released to Monochamus alternatus Hope can effectively prevent and treat Monochamus alternatus Hope, and environmentally safe.
Embodiment
The technology of artificial propagation piebald dastarcus helophoroides of the present invention, its preferable embodiment is to comprise step:
At first, carry out the raising of piebald dastarcus helophoroides adult and bring out, it is laid eggs in a large number; Then, carry out the hatching of ovum, hatch the first-instar young of piebald dastarcus helophoroides; Afterwards, utilize alternative host to raise the first-instar young of piebald dastarcus helophoroides, first-instar young is parasitized substitute host and growth, sprouting wings at last the adult of piebald dastarcus helophoroides.
Wherein, alternative host can be the larva of a kind of longicorn, can be the larva of a kind of overwinter generation longicorn, also can be the larva of a kind of pupa of intending ground beetle or other insect or pupa etc.
Environmental temperature in the said process can be 60% ± 5% for 25 ℃ ± 2 ℃, humidity, also can be the environment of 25 ℃ ± 1 ℃ of temperature, humidity 60% ± 2%.
The raising of the adult of above-mentioned piebald dastarcus helophoroides and bring out to lay eggs and comprise:
At first, (mesh is 2 * 2mm) to onesize white plastic window screening at the bottom of spreading one deck and box at the bottom of the foster worm plastic casing inner box after process is disinfected, put into then and support the worm wooden unit, support worm wooden unit top and put 2～4 layers littler brown paper than wooden unit surface area, a slide is covered in the top of brown paper, and slide, brown paper and wooden unit bundled, support in the worm plastic casing and also be placed with drinking bowl and feed ware.Support the kraft paper bag that also is placed with both ends open in the worm plastic casing, have a rest for the adult lucifuge;
Then, in foster worm plastic casing, insert 20～50 of piebald dastarcus helophoroides adults,, and cover lid, be drilled with a plurality of apertures on the lid, as bore 3～6 apertures as 30,40 first-class; In the piebald dastarcus helophoroides adult, the quantity of female worm can be more than or equal to the quantity of male worm, and the environmental temperature in this process is that 25 ℃ ± 1 ℃, humidity are 60% ± 2%.
Supporting the worm wooden unit is the masson pine wooden unit, an elongated slot has been dug at the middle part of wooden unit, outwards have a stria on the wooden unit surface of one end of elongated slot, brown paper and slide are placed on supports the one side that the worm wooden unit digs elongated slot, elongated slot is covered, and, can be placed with 1 alternative host in the elongated slot of foster worm wooden unit by stria and exterior.In bringing out the process of laying eggs, have the part ovum and hatch larva, and parasitic arriving substitutes on one's body the host.
Making first-instar young parasitize the method that substitutes the host comprises:
At first, get 1 described alternative host, putting into diameter is that 10～15mm, length are in the lucite pipe of 30～90mm; Then, be stained with a little distilled water with the fine, soft fur pen, and according to the size that substitutes the host, pick 2～5 piebald dastarcus helophoroides first-instar youngs of hatching just, be put on alternative host's health, and in described lucite pipe, put into the paper slips that are folded in half into many foldings, be convenient to adhere to when larva is cooked cocoon; Afterwards, with the absorbent cotton jam-pack plastics mouth of pipe.
The adult of piebald dastarcus helophoroides when sprouting wings after, the adult that can sprouting wings carries out routine and protect kind raising, and is conventional and protect kind of the environmental temperature of raising and can be 60% ± 5% for 20 ℃ ± 2 ℃, humidity; Afterwards, needs being brought out the adult that lays eggs repeats described raising and brings out to make its step of laying eggs in a large number; Discharge or refrigerate stand-by not needing to bring out the adult that lays eggs.
Alternative host can carry out fresh-keeping preservation, so that take when needing, specifically comprises:
In the sealing plastic casing, put into one or more wooden units, an elongated slot has been dug at the middle part of wooden unit, be placed with one or the described alternative host in two in the elongated slot, the top ox-hide scraps of paper and the slide of being placed with successively of elongated slot, and wooden unit, brown paper, slide fixed, insert 15～35 of the swollen leg honeybee queen bees of Scleroderma sichuanensis Xiao or Guan Shi in the sealing plastic casing, after 20～24 hours, whether detect alternative host is anaesthetized, take out dopey alternative host and put in the culture dish with cover, and put into 3～5 ℃ low temperature and preserve down standby.Put into new alternative host again, circulation repeatedly.Here used wooden unit can substitute with supporting the worm wooden unit.
The technology of control Monochamus alternatus Hope of the present invention, its preferred implementation is, use the piebald dastarcus helophoroides control Monochamus alternatus Hope of the technology breeding of above-mentioned artificial propagation piebald dastarcus helophoroides, the ovum or the adult of piebald dastarcus helophoroides is released on the tree in Monochamus alternatus Hope harm district.
The egg follicle that discharges the piebald dastarcus helophoroides is drawn together and at first the ovum of piebald dastarcus helophoroides is made ovum and discharged and stick into row and discharge or refrigerate stand-by.The preparation method that ovum discharges card comprises:
At first will have the brown paper that the piebald dastarcus helophoroides of raising laid eggs and cut off by each fan-shaped natural pieces of an egg, piecemeal adheres to the double leaf paper inner face after the doubling then, subsequently doubling double leaf paper is ordered with stapler at opening part, promptly makes ovum and discharges card; Ovum discharges the stand-by temperature of card refrigeration can be 6 ± 1 ℃.
During release, the ovum release card of dastarcus helophoroides is ordered the place, defecation hole of approaching described Monochamus alternatus Hope larva on trunk.
The adult that discharges the piebald dastarcus helophoroides comprises that the adult of piebald dastarcus helophoroides is put into adult release bag or dactylethrae to be discharged or refrigerate stand-by.Refrigerate stand-by temperature and can be 5 ℃ ± 1 ℃.
During release the adult release bag is torn, or after dactylethrae opened, adult directly is poured on butt, let alone freely to crawl into trunk top or diffusion and creep and lay eggs the larva of parasitic Monochamus alternatus Hope or pupa on other tree near on every side.
Specifically can carry out according to the following steps:
Kind of a raising is raised and protected to the routine of step 1, piebald dastarcus helophoroides:
Make the normal development of piebald dastarcus helophoroides and protect the raising of planting.Manufacture insect box earlier, promptly in a plastic casing with cover, (mesh is 2 * 2mm) to onesize white plastic window screening at the bottom of spreading one deck and box at the bottom of the box, put into a foster worm wooden unit then, adult rest paper bag, drinking bowl and a feed ware etc., make an insect box, put into 40 of piebald dastarcus helophoroides adults then, cover plastic box cover (above 5 apertures are arranged be the ventilation hole of 1～2mm); Health in insect box of cleaning in per two days, change feed, adding distil water etc., be placed on 20 ℃ ± 2 ℃, raise in the environment of humidity 60% ± 5%.Afterwards, can carry out step 2 respectively as required breeds; Or step 4 discharges or refrigerates.
The breeding of step 2, piebald dastarcus helophoroides:
The raising of step 2.1, piebald dastarcus helophoroides adult and bringing out is laid eggs:
(mesh is 2 * 2mm) to white plastic window screening onesize at the bottom of shop one deck and box at the bottom of the insect box inner box, put into a foster worm wooden unit that brings out to lay eggs then, replace above-mentioned foster worm wooden unit, put into 30 of piebald dastarcus helophoroides adults (wherein female male worm respectively is about 15) again, cover plastic box cover (above 5 apertures are arranged be the ventilation hole of 1～2mm), be placed in 25 ℃ ± 1 ℃, the environment of humidity 60% ± 2% and raise; After 2 days, bringing out of originally putting into laid eggs support the worm wooden unit and take out, put into newly then, and check whether a small grub in age (health is very little, and only long 1mm is so need pay special attention to observation and searching) of having hatched is arranged in the insect box.
If finding has larva, should manually connect worm immediately and go up artificial propagation to substituting host's (a kind of roundheaded borer or a kind of plan ground beetle pupa), transfer in the feeding process of step 3 larva, and the health in the cleaning insect box, change feed, adding distil water is supported.
The hatching of step 2.2, piebald dastarcus helophoroides ovum:
Bringing out of to taking out from the step 2.1 foster worm wooden unit of being laid eggs in the back of laying eggs generally is put in the plastic casing together with 4, covers plastic box cover (above 5 apertures are arranged be the ventilation hole of 1～2mm); Be placed in 25 ℃ ± 1 ℃, the environment of the constant temperature and humidity of humidity 60% ± 2% and impel the ovum hatching, checked in a plastic casing whether the piebald dastarcus helophoroides first-instar young that has hatched is arranged in per 2 days,, just transfer in the feeding process of larva if find that larva is arranged.If there is not to find then to proceed to make the program of ovum hatching.
After the step 2.1, also can not carry out the hatching of step 2.2 ovum, discharge card and discharge immediately or refrigerate stand-by and ovum is made ovum.
Foster worm wooden unit in the foster worm wooden unit that bringing out of mentioning in the step 2 laid eggs and the step 1 can be with identical wooden unit, supports in the elongated slot at worm wooden unit middle part and put into an alternative host, promptly can be made into and brings out the foster worm wooden unit of laying eggs.
The raising of step 3, piebald dastarcus helophoroides larva:
To have the foster worm wooden unit of the piebald dastarcus helophoroides first-instar young of firm hatching to open in the insect box of having found, the piebald dastarcus helophoroides larva in the insect box manually connects worm immediately.At first an alternative host is put into the transparent plastic dactylethrae of clean diameter 12mm, the long 80mm that has also sterilized, every pipe 1 worm, it is 1 bundle that per 4 dactylethraes are pricked with rubber band, be put in another plastic casing and raise, lid not, connect piebald dastarcus helophoroides first-instar young then, tampon beyond the Great Wall places 25 ℃ ± 2 ℃, the environment of humidity 60% ± 5% to raise.Check after general 40 days, see if there is piebald dastarcus helophoroides adult eclosion of new generation and go out.The piebald dastarcus helophoroides adult that newly sprouts wings is carried out instant-free or enter step 4 packing and refrigeration; Replacing of not sprouting wings continues to raise.
And will bring out in the foster worm wooden unit of laying eggs and be taken out by the alternative host of piebald dastarcus helophoroides larva parasitism, be put in the culture dish, change the alternative host larva of 1 work again, be put into to support to make in the worm wooden unit and bring out the foster worm wooden unit of laying eggs, it is put back in this insect box, continues to enter into the hatching process of ovum;
Packing and the refrigeration of step 4, piebald dastarcus helophoroides adult: the adult that will newly sprout wings 1 program of raising is set by step raised, and adult is supplemented the nutrients.Raise and divide male and female after 5 days, each 5 of female male worms are put in the adult release bag, seal the endways plastic casing of putting into.Put about 140 adult release bags in each plastic casing, again plastic casing is put into 4 ℃～6 ℃ low temperature refrigeration down.During perhaps piebald dastarcus helophoroides adult is packed the dactylethrae with cover of long 50mm, aperture 12mm into, cover the ventilation hole that bores an aperture 1mm, every pipe is adorned 10 adults.Instant-free if desired if temporarily do not discharge, then refrigerates stand-by.The temperature of refrigeration is 6 ± 1 ℃.
The release of step 5, piebald dastarcus helophoroides:
Select to discharge adult before at 12 o'clock in dusk to evening of sunny weather.Per 3 trees that are injured are put 1 an adult release bag or a dactylethrae (i.e. 10 adults).During release, will pour into the usage tree root of being injured behind the release bag tearing notch, or the dactylethrae mouth of pipe opened piebald dastarcus helophoroides adult is poured on butt.Can see that subsequently piebald dastarcus helophoroides adult climbs on the trunk very soon, seek Monochamus alternatus Hope larva defecation hole.Female worm generally lays eggs under bark crack on every side, longicorn defecation hole, can easily enter into the parasitic host in Monochamus alternatus Hope larva tunnel after making things convenient for small grub to hatch.Discharging suitable time is 3 ages of Monochamus alternatus Hope and later larval phase.
Below to some operation, the specific embodiment of apparatus and the detailed description of predicate thereof in the above steps:
Plastic casing: can be the transparent plastic box with cover of length * wide * height=17.5 * 11.5 * 6cm, need to dip in alcohol (75%) before using, the surface in the box be carried out disinfection, and then be stained with distilled water, clean the box surfaces externally and internally with absorbent cotton with absorbent cotton.
Support the worm wooden unit: the masson pine trees section that can be length * wide * height=8.4 * 4.5 * 3.5cm, the groove of cutter one long * wide * dark=50 * 15 * 15mm in the middle of above the juggle, the place ahead of groove is to tiny groove of external-open, on groove, put 3～4 on the ox-hide scraps of paper of 80 * 30mm, putting a length and width height on brown paper again is the slide of 75 * 25 * 2mm, rests worm wooden unit, brown paper and slide with rubber band at last and is locked.
Adult rest paper bag: with long * wide be the brown paper of 4.5 * 8.5cm, peg outer rim with stapler after the doubling, allow piebald dastarcus helophoroides lucifuge and " rest " therein.
Drinking bowl: can be respectively the transparent plastic dactylethrae of 40mm, 13mm with long and aperture, interior Sheng distilled water (account for manage internal volume 2/3) is filled in the mouth of pipe with sponge then.
Feed ware: diameter and highly be respectively the vinyl cover of 13mm, 5mm.The interior artificial feed of containing are got food for the hard first of piebald flower suede.
Artificial feed: with the oven dry earlier of roundheaded borer, yellow meal worm and masson pine bark cambium, pulverizing then, add multiple trace element again, in 1: 1: 0.4: 0.2 ratio mixed, and each preparation is too much unsuitable, be placed on after configuring preserve below 0 ℃ standby.
Bring out the foster worm wooden unit that piebald dastarcus helophoroides adult lays eggs in a large number: get a foster worm wooden unit made from pinus massoniana, in the sulculus of centre, put into the chips of some horse hair pine trees, put the real absorbent cotton of also using stifled real, again on the absorbent cotton a little distilled water to the tiny groove of external-open.Get 1 alternative host's larva and put in the groove, cover groove, push down brown paper with slide again, with rubber band slide, brown paper and foster worm juggle are put with fixing then with the ox-hide scraps of paper; Used alternative host is a kind of roundheaded borer of overwinter generation, and collection and storage are all easier, and cost is low.
Manually connect worm: get 1 alternative host earlier, put into diameter and be respectively in the transparent plastic dactylethrae of 13mm and 40mm, dip in the fine, soft fur pen earlier and get a little distilled water, pick firm hatching piebald dastarcus helophoroides first-instar young soon with high, be put into gently on the health that substitutes the host, promptly finish connecing worm.Connect the worm amount and decide, generally connect the hard first larva of 2～3 first piebald flower suedes in age according to the individual size that substitutes the host.The body weight that substitutes the host connects 2 headdress flower spot dastarcus helophoroides larvas and is advisable between 0.15～0.30g the time; The body weight that substitutes the host connects 3 headdress flower spot dastarcus helophoroides larvas and is advisable between 0.30～0.45g the time.After connecing worm, the 70g rectangle brown paper that length and width are respectively 30mm and 15mm is converted into 4 foldings and puts into pipe, near alternative host, weaves silk after aging in order to piebald dastarcus helophoroides larva and to adhere to when spinning, and uses the absorbent cotton jam-pack mouth of pipe at last.The degree that absorbent cotton stops up is the plastic tube degree of being that do not come off to pinch absorbent cotton with hand.
Substitute host's preparation: 2 alternative hosts can be put into and support in the middle groove of worm wooden unit, the brown paper of wide 80 * 30mm lengthens on groove, the scantling that lengthens again is the slide of 75 * 25 * 2mm, puts with fixing with rubber band then, puts in the plastics insect box.Put into 4 of so foster worm wooden units in the plastic casing, insert 25 of the swollen leg honeybee queen bees of Scleroderma sichuanensis Xiao or Guan Shi then, cover lid (cover and do not have ventilation hole), after 20～24 hours, check whether the alternative host who supports in the worm wooden unit anaesthetizes to be stung by swollen leg sting.The alternative host of anesthesia taken out put into diameter and highly be respectively in the culture dish with cover of 65mm, 25mm, change to new alternative host again, so circulation obtains a large amount of alternative hosts that anaesthetized, to be equipped with again and anaesthetize the culture dish that substitutes the host, and put into 3～5 ℃ low temperature and preserve standby down.
Adult release bag: can be the brown paper of 85 * 60mm with length and width, be folded into the rectangle pouch of 65 * 35mm, then an opening is pegged earlier with stapler from distance opening 5mm is folding, behind 10 of the dastarcus helophoroides adults of packing into, another opening is folded from distance opening 5mm peg again.
The invention solves the topmost artificial mass rearing of natural enemy insect piebald dastarcus helophoroides and the breeding problem of Monochamus alternatus Hope, prevent and treat Monochamus alternatus Hope for using and promote the piebald dastarcus helophoroides aborning, finally reaching the control pine nematode provides a kind of important natural enemy resource; And worked out artificial mass rearing and discharged the technology and the method for this natural enemy, especially discharge the Prevention Technique (all being to discharge adult in the past basically in the prior art) of ovum when utilizing the natural enemy pest control, greatly reduce the reproductive-cost of natural enemy, improved the breeding amount of natural enemy, and had and the identical control efficiency of release adult.The present invention system utilizes the natural enemy insect Pest Control, accomplish under the prerequisite of free from environmental pollution, maintaining ecological balance and maintenance bio-diversity, insect and disease have been obtained effectively and the control that continues, be the advanced technology of control Monochamus alternatus Hope and pine nematode, at present at home and abroad also beyond example.
Specific embodiment, artificial mass rearing, breeding and the release of piebald dastarcus helophoroides:
1. raise:
Refer to conventional and the raising of protecting kind.Manufacture insect box earlier, promptly (long * wide * high=17.5 * 11.5 * 6cm) in a plastic casing with cover, (mesh is 2 * 2mm) to first white plastic window screening onesize at the bottom of bottom shop one deck and box, it is (long * wide * high=8.4 * 4.5 * 3.5cm), adult rest paper bags, drinking bowl and feed wares to put into a foster worm wooden unit then, make an insect box, put into 30 of piebald dastarcus helophoroides adults again, cover plastic box cover (above 5 apertures are arranged be 1～2mm ventilation hole); Health in insect box of cleaning in two days is changed feed, and adding distil water is placed on temp. and humidity and is respectively in 20 ℃ ± 2 ℃ and 60% ± 5% the environment and raises.
2. breeding:
1) raising of adult and bringing out is laid eggs: in an insect box, put into a foster worm wooden unit that brings out to lay eggs, put into 30 of piebald dastarcus helophoroides adults (each 15 of male and female) again, cover plastic box cover (above 5 apertures are arranged be the ventilation hole of 1～2mm); Health in the cleaning insect box is changed feed, adding distil water; Be placed in the constant-temperature constant-humidity environment of 25 ℃ ± 1 ℃ of temperature, humidity 60% ± 2% and raise.After 2 days, take out the foster worm wooden unit laid eggs, change to newly, and check in the insect box whether the dastarcus helophoroides small grub in one age of having hatched is arranged.If finding has larva, manually connect worm immediately, and transfer in the process of larva raising
2) hatching of ovum: with the foster worm wooden unit that ovum is arranged of above-mentioned taking-up, per 4 are put in the new plastic box together, and (above 2～5 1～2mm ventilation holes are arranged) closes the lid; Be placed in the constant-temperature constant-humidity environment of 25 ℃ ± 1 ℃ of temperature, humidity 60% ± 2% and raise, checked in a plastic casing whether the larva that has hatched is arranged in per 4 days,, just transfer in the feeding process of larva if find that larva is arranged.If not finding has larva, proceed the hatching of ovum.
3) raising of larva: will find to have in the plastic casing the foster worm wooden unit of ovum that lures of larva to open, to be taken out by the alternative host of piebald dastarcus helophoroides larva parasitism, being put into a diameter clean and that sterilized is respectively in the culture dish of 80mm and 12mm with high, change the alternative host larva of 1 work again, be put into to support to make in the worm wooden unit and bring out the foster worm wooden unit of laying eggs, this foster worm wooden unit is put back in the new plastic casing, carries out the hatching of ovum; To in the plastic casing and alternative host's health on larva, manually connect worm immediately, the lucite pipe of worm will be connected, per 4 is 1 bundle with the rubber band bundle, puts in the new plastic casing, not lid, be placed on again in the constant-temperature constant-humidity environment of 25 ℃ ± 2 ℃ of temperature, humidity 60% ± 5% and raise, check after 40 days whether adult eclosion is arranged, and the adult that sprouts wings packed and refrigerates that replacing of not sprouting wings continues to raise.
4) packing of adult and refrigeration: the adult that will newly sprout wings is raised with the raising program that the guarantor plants routinely, and adult is supplemented the nutrients.Raise and divide male and female after 5 days, with 5 male worms, 5 female worms are put in the release bag, seal, and endwaysly put into new plastic casing, put 140 left and right sides release bags in each plastic casing, plastic casing are put into 4 ℃～6 ℃ low temperature refrigeration down again.
3. discharge:
1) discharges adult: discharge adult when selecting dusk of sunny weather.Per 3 trees that are injured are put 1 adult release bag (i.e. 10 adults).During release, pour into the usage tree root of being injured after release bag torn a mouth, can see that at this moment piebald dastarcus helophoroides adult crawls on the trunk very soon, seek Monochamus alternatus Hope larva defecation hole.Female worm generally lays eggs under the bark crack around longicorn defecation hole, can make things convenient for after small grub hatches from ovum and easily enters into the parasitic host in Monochamus alternatus Hope larva tunnel.Discharging suitable time is 3 ages of Monochamus alternatus Hope and later larval phase.
2) discharging piebald dastarcus helophoroides ovum that ovum obtains artificial feeding earlier makes ovum and discharges card (every card has 50～350 on ovum not wait, and depends on the quantity according to ovum on the once actual pieces of an egg that produced of a female worm).During release ovum release is blocked with stapler nail around the place, defecation hole of Monochamus alternatus Hope larva on the trunk that is subjected to longicorn harm.Ovum can climb in the Monochamus alternatus Hope tunnel after hatching into first-instar young voluntarily, and is parasitic on its larva and pupa.
After the piebald dastarcus helophoroides discharged, control efficiency was remarkable: discharge then, in the test standing forest parasitic rate of Monochamus alternatus Hope larva is reached the control efficiency that reached basic controlling in 56.3%, the second year.
Main natural enemy---the piebald dastarcus helophoroides (Dastarcus sp.) (coleoptera: post the Bothrideridae of first section) of parasitic Monochamus alternatus Hope larva and pupa.It is in the masson pine standing forest of pine nematode serious harm, the general nature parasitic rate is lower, be about 3%, but the natural parasitic rate on the Monochamus alternatus Hope of indivedual masson pine plant illustrate that up to 79.1% the piebald dastarcus helophoroides is the important natural enemy of nature control Monochamus alternatus Hope.And, be the unique a kind of topmost natural enemy that to control Monochamus alternatus Hope through our investigation for many years.As a kind of natural enemy resource, can be used in and produce control, most critical be can breed a large amount of individualities to discharge, just can reach the effect of Pest Control.Therefore, how to solve artificial mass rearing and propagation technique and just become key.The invention solves a large amount of propagation techniques of this natural enemy, and discharge a Prevention Technique difficult problem, provide pollution-free, an environmentally safe biological control new technology for controlling Monochamus alternatus Hope effectively constantly and then controlling pine nematode.
Use the present invention, generally in most cases at woodland, 2 parasitic 1 Monochamus alternatus Hope larva or pupas eliminated of dastarcus helophoroides, the life-span of the hard first adult of piebald flower suede is long, reach 3～5 years, and reproduction speed is fast, utilizes piebald dastarcus helophoroides control Monochamus alternatus Hope to have very wide prospect.
The above; only for the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, and anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (10)
1, a kind of technology of artificial propagation piebald dastarcus helophoroides is characterized in that, comprises step:
At first, the raising and bringing out of carrying out the adult of described piebald dastarcus helophoroides is laid eggs;
Then, carry out the hatching of ovum, hatch the first-instar young of described piebald dastarcus helophoroides;
Afterwards, utilize alternative host to raise the first-instar young of described piebald dastarcus helophoroides, make described first-instar young parasitize described alternative host and growth, sprouting wings at last the adult of described piebald dastarcus helophoroides, and described alternative host is larva or a kind of pupa of intending ground beetle of a kind of longicorn;
Environmental temperature in the said process is that 25 ℃ ± 2 ℃, humidity are 60% ± 5%.
2, the technology of artificial propagation piebald dastarcus helophoroides according to claim 1 is characterized in that, the raising of the adult of described piebald dastarcus helophoroides and bring out to lay eggs and comprise:
At first, in through the foster worm plastic casing after disinfecting, put into and support the worm wooden unit, 2～4 layers littler than wooden unit surface area brown paper is put in described foster worm wooden unit top, a slide is covered in the top of described brown paper, and described slide, brown paper and wooden unit bundled, also be placed with drinking bowl and feed ware in the described foster worm plastic casing, also be placed with the kraft paper bag for the dastarcus helophoroides rest of both ends open in the described foster worm plastic casing;
Then, in described foster worm plastic casing, insert 20～50 of described piebald dastarcus helophoroides adults, and cover lid, be drilled with 3～6 apertures on the described lid; In the described piebald dastarcus helophoroides adult, the quantity of female worm is more than or equal to the quantity of male worm;
The raising of described adult and bringing out is laid eggs and carried out environmental temperature in the process of hatching of ovum is that 25 ℃ ± 1 ℃, humidity are 60% ± 2%.
3, the technology of artificial propagation piebald dastarcus helophoroides according to claim 2, it is characterized in that, described foster worm wooden unit is the masson pine wooden unit, an elongated slot has been dug at the middle part of described masson pine wooden unit, outwards have a stria on the wooden unit surface of one end of described elongated slot, described brown paper is placed on the one side that described foster worm wooden unit digs elongated slot, is placed with 1 described alternative host in the elongated slot of described foster worm wooden unit.
4, the technology of artificial propagation piebald dastarcus helophoroides according to claim 1 is characterized in that, the method that makes described first-instar young parasitize described alternative host comprises:
At first, get 1 described alternative host, putting into diameter is that 10～15mm, length are in the lucite pipe of 30～90mm;
Then, be stained with a little distilled water with the fine, soft fur pen, and, pick 2～5 described piebald dastarcus helophoroides first-instar youngs of hatching just according to described alternative host's size, be put on described alternative host's health, and in described lucite pipe, put into the brown paper paper slip that is folded in half into many foldings;
Afterwards, with the stifled tight described transparent plastic mouth of pipe of absorbent cotton.
5, the technology of artificial propagation piebald dastarcus helophoroides according to claim 1, it is characterized in that, after the adult of described piebald dastarcus helophoroides when sprouting wings, also the adult that sprouting wings carries out routine and protects kind raising, described routine and to protect kind of an environmental temperature of raising be that 20 ℃ ± 2 ℃, humidity are 60% ± 5%;
Afterwards, needs being brought out the adult that lays eggs repeats described raising and brings out the step of laying eggs; Discharge or refrigerate stand-by not needing to bring out the adult that lays eggs.
6, the technology of artificial propagation piebald dastarcus helophoroides according to claim 1 is characterized in that, also comprises described alternative host's fresh-keeping preservation, specifically comprises:
In the sealing plastic casing, put at least one wooden unit, an elongated slot has been dug at the middle part of described wooden unit, be placed with one or the described alternative host in two in the elongated slot, the top ox-hide scraps of paper and the slide of being placed with successively of described elongated slot, and bundle described wooden unit, brown paper, slide fixing, insert 15～35 of the swollen leg honeybee queen bees of Scleroderma sichuanensis Xiao or Guan Shi in the described sealing plastic casing, after 20～24 hours, take out dopey alternative host and put in the culture dish with cover, and put into 3～5 ℃ low temperature and preserve down standby.
7, a kind of technology of preventing and treating Monochamus alternatus Hope, it is characterized in that, application rights requires the piebald dastarcus helophoroides control Monochamus alternatus Hope of the technology breeding of 1 to 6 each described artificial propagation piebald dastarcus helophoroides, and the ovum or the adult of described piebald dastarcus helophoroides is released on the tree in described Monochamus alternatus Hope harm district.
8, the technology of control Monochamus alternatus Hope according to claim 7 is characterized in that, the egg follicle that discharges described piebald dastarcus helophoroides is drawn together and the ovum of described piebald dastarcus helophoroides is made ovum discharged and stick into row and discharge or refrigerate stand-byly, and the making that described ovum discharges card comprises:
The brown paper that at first will have the ovum that the piebald dastarcus helophoroides of raising produced is cut off by each fan-shaped natural pieces of an egg piecemeal, adheres to the double leaf paper inner face after the doubling then, subsequently described doubling double leaf paper is ordered with stapler at opening part, promptly makes described ovum and discharges card;
It is 6 ± 1 ℃ that described ovum discharges the stand-by temperature of card refrigeration.
9, the technology of control Monochamus alternatus Hope according to claim 8 is characterized in that, the ovum of described dastarcus helophoroides is discharged stick into row and discharge, and comprises that described ovum is discharged card orders on trunk place, defecation hole near described Monochamus alternatus Hope larva.
10, the technology of control Monochamus alternatus Hope according to claim 7, it is characterized in that, the adult that discharges described piebald dastarcus helophoroides comprises, the adult of described piebald dastarcus helophoroides is put into adult release bag or dactylethrae discharge or refrigerate stand-byly, the method for releasing of described adult release bag or dactylethrae comprises:
After described adult release bag being torn or dactylethrae being opened, adult directly is poured on the butt of Monochamus alternatus Hope larva harm, let alone freely to crawl into trunk top or diffusion and creep that other tree goes up egg reproduction, the larva of parasitic Monochamus alternatus Hope or pupa near on every side;
The stand-by temperature of described adult release bag refrigeration is 5 ℃ ± 1 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101141815A CN101589697B (en) | 2008-05-30 | 2008-05-30 | Captive-breed piebald dastarcus helophoroides and method applied to prevent and control monochamus alternatus hope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101141815A CN101589697B (en) | 2008-05-30 | 2008-05-30 | Captive-breed piebald dastarcus helophoroides and method applied to prevent and control monochamus alternatus hope |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101589697A true CN101589697A (en) | 2009-12-02 |
| CN101589697B CN101589697B (en) | 2013-05-01 |
Family
ID=41404727
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101141815A Expired - Fee Related CN101589697B (en) | 2008-05-30 | 2008-05-30 | Captive-breed piebald dastarcus helophoroides and method applied to prevent and control monochamus alternatus hope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101589697B (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524184A (en) * | 2011-12-22 | 2012-07-04 | 段东红 | Preparation method of composite natural enemy spray |
| CN103109712A (en) * | 2013-03-20 | 2013-05-22 | 遵义市林业科学研究所 | Method for reducing monochamus alternatus hope density and increasing dastarcus helophoroides density in woods |
| CN103271022A (en) * | 2013-06-05 | 2013-09-04 | 新疆林科院森林生态研究所 | Method for controlling melanophila picta pallas larvae through fusarium proliferatum carried on sclerodermus pupariae |
| CN103355260A (en) * | 2013-07-30 | 2013-10-23 | 天津拓华生物科技有限责任公司 | Application of thyestillageblerifacldermann larva as host to artificial propagation of dastarcushelophoroides |
| CN103828791A (en) * | 2014-01-17 | 2014-06-04 | 徐华潮 | Method for preventing and treating pecan batocera horsfieldi with dastarcus helophoroides |
| WO2015017946A1 (en) * | 2013-08-07 | 2015-02-12 | Li Jianqing | Dastarcus helophoroides imago releasing box |
| CN104871886A (en) * | 2015-06-25 | 2015-09-02 | 中国林业科学研究院森林生态环境与保护研究所 | Method for effectively controlling bursaphelenchus xylophilus by biologically controlling monochamus alternatus |
| CN104920308A (en) * | 2015-06-25 | 2015-09-23 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificial breeding of larch sclerodermus sp. and application of larch sclerodermus sp. for prevention of massicus raddei |
| CN104996362A (en) * | 2015-06-25 | 2015-10-28 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificially breeding sclerodermus pupariae yang et yao and applying sclerodermus pupariae yang et yao to control trunk-boring insects |
| CN105830824A (en) * | 2016-03-23 | 2016-08-10 | 来燕学 | Biological control method for bursaphelenchus xylophilus |
| CN108575915A (en) * | 2018-04-18 | 2018-09-28 | 广州威佰昆生物科技有限公司 | Cold storage anesthesia transportation release method for mosquitoes |
| CN112400811A (en) * | 2020-11-25 | 2021-02-26 | 江苏省林业科学研究院 | Method for breeding dastarcus helophoroides on large scale by utilizing thyestillageblerifolius |
| CN112616778A (en) * | 2020-12-12 | 2021-04-09 | 四川农业大学 | Method for keeping parasitic capacity of artificially fed Szechwan scleroderma and application of method |
-
2008
- 2008-05-30 CN CN2008101141815A patent/CN101589697B/en not_active Expired - Fee Related
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524184A (en) * | 2011-12-22 | 2012-07-04 | 段东红 | Preparation method of composite natural enemy spray |
| CN103109712A (en) * | 2013-03-20 | 2013-05-22 | 遵义市林业科学研究所 | Method for reducing monochamus alternatus hope density and increasing dastarcus helophoroides density in woods |
| CN103271022A (en) * | 2013-06-05 | 2013-09-04 | 新疆林科院森林生态研究所 | Method for controlling melanophila picta pallas larvae through fusarium proliferatum carried on sclerodermus pupariae |
| CN103271022B (en) * | 2013-06-05 | 2015-01-21 | 新疆林科院森林生态研究所 | Method for controlling melanophila picta pallas larvae through fusarium proliferatum carried on sclerodermus pupariae |
| CN103355260A (en) * | 2013-07-30 | 2013-10-23 | 天津拓华生物科技有限责任公司 | Application of thyestillageblerifacldermann larva as host to artificial propagation of dastarcushelophoroides |
| CN103355260B (en) * | 2013-07-30 | 2016-04-13 | 天津拓华生物科技有限责任公司 | The perpendicular hair roundheaded borer of fiber crops is as the application of host in dastarcus helophoroides mass rearing |
| WO2015017946A1 (en) * | 2013-08-07 | 2015-02-12 | Li Jianqing | Dastarcus helophoroides imago releasing box |
| CN103828791A (en) * | 2014-01-17 | 2014-06-04 | 徐华潮 | Method for preventing and treating pecan batocera horsfieldi with dastarcus helophoroides |
| CN104920308A (en) * | 2015-06-25 | 2015-09-23 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificial breeding of larch sclerodermus sp. and application of larch sclerodermus sp. for prevention of massicus raddei |
| CN104996362A (en) * | 2015-06-25 | 2015-10-28 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificially breeding sclerodermus pupariae yang et yao and applying sclerodermus pupariae yang et yao to control trunk-boring insects |
| CN104871886A (en) * | 2015-06-25 | 2015-09-02 | 中国林业科学研究院森林生态环境与保护研究所 | Method for effectively controlling bursaphelenchus xylophilus by biologically controlling monochamus alternatus |
| CN104871886B (en) * | 2015-06-25 | 2017-03-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of method of Biological control Monochamus alternatus Hope effective control pine nematode |
| CN104996362B (en) * | 2015-06-25 | 2018-01-26 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of swollen leg honeybee of artificial breeding Chinese wax Ji fourth and the method applied to preventing and treating trunk borer |
| CN105830824A (en) * | 2016-03-23 | 2016-08-10 | 来燕学 | Biological control method for bursaphelenchus xylophilus |
| CN108575915A (en) * | 2018-04-18 | 2018-09-28 | 广州威佰昆生物科技有限公司 | Cold storage anesthesia transportation release method for mosquitoes |
| CN112400811A (en) * | 2020-11-25 | 2021-02-26 | 江苏省林业科学研究院 | Method for breeding dastarcus helophoroides on large scale by utilizing thyestillageblerifolius |
| CN112616778A (en) * | 2020-12-12 | 2021-04-09 | 四川农业大学 | Method for keeping parasitic capacity of artificially fed Szechwan scleroderma and application of method |
| CN112616778B (en) * | 2020-12-12 | 2022-05-03 | 四川农业大学 | Method for keeping parasitic capacity of artificially fed Szechwan scleroderma and application of method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101589697B (en) | 2013-05-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101589697B (en) | Captive-breed piebald dastarcus helophoroides and method applied to prevent and control monochamus alternatus hope | |
| Tubbs | Manager's handbook for northern hardwoods in the North Central States | |
| Baker | Eastern forest insects | |
| CN104067989B (en) | Wasp cultural method | |
| CN101574075A (en) | Artificial propagation technology of mallambyx raddei dastarcus helophoroides and technology for applying dastarcus helophoroides for controlling mallambyx raddei | |
| Arnett et al. | Simon and Schuster's guide to insects | |
| CN101622971A (en) | Technology for artificially raising and breeding Dastarcus helophoroides of Anoplophora malasiaca and preventing and controlling Anoplophora glabripennis | |
| CN103478117B (en) | The diffusion breeding method for releasing of a kind of harmonia axyridia control cigarette aphid | |
| CN101589696B (en) | Technique for artificial propagation of apriona dastarcus helophoroides and preventive treatment of apriona swainsoni hope | |
| CN103299956A (en) | Technology for artificial domestication and breeding of sclerodermus and applied to prevention and treatment of pine sawyer beetles | |
| CN102499188B (en) | Agasicles hygrophila experimental population raising method | |
| CN101683069A (en) | Artificial propagated batocera lineolata dastarcus helophoroides and technology for preventing and treating batocera lineolata | |
| Blackman | I. Notes on insects bred from the bark and wood of the American larch | |
| CN107581159A (en) | A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee | |
| CN103858827A (en) | Method for carrying out indoor large-scale diadegma semiclausum hellen breeding | |
| CN105830824A (en) | Biological control method for bursaphelenchus xylophilus | |
| Perera et al. | Conservation attempt of critically endangered mangrove Lumnitzera littorea (Jack) Voigt in Madu Ganga Ramsar Site of Sri Lanka; stand composition and seed germination | |
| Anderson | Rose canker and its control | |
| Burakowski | Laboratory methods for rearing soil beetles (Coleoptera) | |
| Paine et al. | Integrated program protects trees from eucalyptus longhorned borer | |
| Thu et al. | Healthy plantations. A field guide to pests and pathogens of Acacia, Eucalyptus and Pinus in Vietnam. | |
| CN112400811A (en) | Method for breeding dastarcus helophoroides on large scale by utilizing thyestillageblerifolius | |
| van der Burg et al. | Seed germination methods for native Caribbean trees and shrubs: with emphasis on species relevant for Bonaire | |
| Mauchline et al. | Life history and abundance of the eucalyptus leafroller (Strepsicrates macropetana) | |
| Spencer et al. | Series Entomologica |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130501 Termination date: 20160530 |