CN105830824A - Biological control method for bursaphelenchus xylophilus - Google Patents
Biological control method for bursaphelenchus xylophilus Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
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Abstract
The invention discloses a biological control method for bursaphelenchus xylophilus. The biological control method comprises the following steps: firstly, taking a manually set device for trapping dastarcus helophoroide eggs to collect eggs of dastarcus helophoroides so as to obtain egg cards; placing part of the egg cards in a culture box, and performing cultivation to obtain chrysalises; and after the chrysalises emerge into bursaphelenchus xylophilus imagoes in 20-30 days, collecting the imagoes into a container to obtain imagoes. Pine disease wood piles are arranged in each bursaphelenchus xylophilus affected area, the egg cards or imagoes are put into the pine disease wood piles in March and April every year and the pine disease wood piles are enwrapped by an isolation material for isolation. Compared with the prior art, according to the biological control method disclosed by the invention, epidemic situations are controlled on the spot, and an affected area is changed into a place for naturally breeding dastarcus helophoroides, so that the labor intensity of epidemic prevention is greatly reduced, the epidemic prevention efficiency is improved, the epidemic prevention effect can be ensured for many years after the residence of the dastarcus helophoroides, and the biological control method is suitable for controlling bursaphelenchus xylophilus in mountain woods and forests.
Description
Technical field
The present invention relates to field of biological control, particularly relate to be applicable to the biological control method of the pine line disease of Mountainous Area and forest.
Background technology
Forest is the biome based on arbor, be concentrate arbor and other plant, animal, microorganism and soil between mutual dependence for existence mutually restrict, and with environmental interaction and form the overall of an ecosystem.Forest has abundant species, and complicated structure and diversified function, the existence to the mankind is significant, and is described as " lung of the earth ".In the mountain forest in Subtropic of China area, pinaster is numerous, pine nematode is the crushing epidemic diseases of a kind of pinaster, and during generation, pine forest Chang Lianmian is injured, the most withered, not only the area that is injured can be caused serious economic loss, and the ecological balance in area can be caused serious destruction.Owing to pine forest is distributed in high and steep mountains more, Masson Pine tree height is big in addition, once pest and disease damage occurs, prevents and treats extremely difficult.Current existing many preventing and treating apparatuses all cannot use under the dangerously steep environmental condition in mountain region, also cannot drugs play a role to tree crown, and the most relatively costly with aircraft spraying medicine, and it is inaccurate to will also result in dispenser, kills the problem such as natural enemy and environmental pollution.In a word, Bursaphelenchus xylophilus preventing and treating has three big difficult points: one is that pine forest area is big, and condition is poor vegetatively, is often distributed in high and steep mountains, apprentice precipice cliff, and routine techniques is difficult to put in place；Two is that chemicals is difficult to penetrate Monochamus alternatus Hope and the Bursaphelenchus xylophilus killing deep plant timber depths；Three be pine nematode be international quarantine object, so preventing and treating time in addition to need to killing Monochamus alternatus Hope, also to have suitable quarantine measures.
In ecosystem, forming food chain between various biologies due to food, by food chain relation, utilizing natural enemy insect to prevent and treat insect is the research emphasis of pest and disease damage Biological control in current agriculture and forestry.The wound that in pine nematode, Bursaphelenchus xylophilus is supplemented the nutrients by Monochamus alternatus Hope enters xylem, colonize in resin canal, amount reproduction is the most mobile, gradually throughout Herb, and cause resin canal parenchyma cell and the destruction of epithelial cell and death, cause plant dehydration, transpiration reduces, resin secretion drastically reduces and stops, and the external symptom that plant shows is that needle becomes yellowish-brown or even bronzing, wilting successively, and last whole strain is withered.The cause of disease of pine nematode is Bursaphelenchus xylophilus (Bursaphelenchus xylophilus), is the great destructive disease of pinaster, it has also become first of forest disease and pest.At present, the common recognition of forestry circle and control strategy are: it is first necessary to effectively control the communication media Monochamus alternatus Hope of Bursaphelenchus xylophilus, could effectively contain pine nematode.The Monochamus alternatus Hope most of the time throughout one's life is larval stage, takes food in trunk, battalion's disguise life, in prior art, main combination by chemical prevention and other conventional control methods is prevented and treated, but this kind of method is difficult to effectively prevent and treat Monochamus alternatus Hope, and pollutes environment.The natural enemy insect utilizing Monochamus alternatus Hope is the most emerging a kind of biological control method to control Monochamus alternatus Hope thus to prevent and treat pine nematode, dastarcus helophoroides imago can prey on the larva of Monochamus alternatus Hope, prepupa and pupa, and its larva can colonize in larva and the prepupa of Monochamus alternatus Hope.
Application No. 200810114181.5 (Authorization Notice No. is CN 101589697 B) Chinese invention patent " artificial propagation piebaldism dastarcus helophoroides and the method being applied to prevent and treat Monochamus alternatus Hope " discloses a kind of method for preventing and treating Monochamus alternatus Hope, first ambient temperature be 25 DEG C ± 2 DEG C, humidity be 60% ± 5% environment in the adult of piebaldism dastarcus helophoroides raised and induction makes it lay eggs in a large number, and hatch the larva of piebaldism dastarcus helophoroides；Then utilize Hosts to make its parasitic, growth, finally sprout wings into adult, the ovum of piebaldism dastarcus helophoroides or adult can be made ovum release card or adult release bag afterwards or dactylethrae is released to the epidemic-stricken area that the Monochamus alternatus Hope in pine nematode epidemic-stricken area endangers.This patent utilization natural enemy insect controls insect, can be on the premise of free from environmental pollution, maintaining ecological balance and holding bio-diversity, control Monochamus alternatus Hope and pine nematode, but relatively low to Monochamus alternatus Hope parasitic rate (56.3%), directly it is released to be injured on plant by ovum or the adult of dastarcus helophoroides during release, being injured and isolate between plant and healthy plant, prevention effect is poor.
Summary of the invention
The technical problem to be solved is to provide a kind of to be applicable to Mountainous Area and forest and the sick biological control method of the good pine line of prevention effect for prior art.
The present invention solves the technical scheme that above-mentioned technical problem used: the biological control method that a kind of pine line is sick, it is characterised in that comprise the following steps:
(1) take a bit of in have the Masson Pine of Monochamus alternatus Hope larva, carry out binding with multiwall kraft after wrapping up fixing, dastarcus helophoroides, in Corii Bovis seu Bubali laying eggs on paper, collects the ovum on kraft paper, obtains ovum card；
Take part ovum to be placed in cultivation box, it is placed in the transparent tube being loaded with Monochamus alternatus Hope maturation larva with the ovum on spicule picking ovum card, mouth of pipe defat tampon blocks up, after one week, when dastarcus helophoroides egg hatching is 1-instar larvae, 1-instar larvae is quickly familiar with the body odour of Monochamus alternatus Hope larva and to Monochamus alternatus Hope parasitism, now dastarcus helophoroides larva grows on Monochamus alternatus Hope body, cocooing until weaving silk, during pupa, obtaining cocoon pupa；
Cocoon pupa, through 20～30 days, turns into dastarcus helophoroides imago, is collected in container by adult, obtains adult.
(2) the loose epidemic disease wood felling in pine line disease epidemic-stricken area is classified as pine epidemic disease yard, every heap comprises 4～6 loose epidemic disease wood, this pine epidemic disease yard is distributed in pine line disease epidemic-stricken area, in between annual 3～April, described ovum card or adult it is invested in pine epidemic disease yard and isolates with isolated material parcel pine epidemic disease yard, there is on this pine epidemic disease wood Monochamus alternatus Hope larva moth hand-hole and there is no emergence hole, described isolated material has and can block Monochamus alternatus Hope and the freely through mesh of dastarcus helophoroides imago can be made.
Described isolated material is woven cloth or nylon wire or wire gauze, these isolated materials are conveniently easy to get, cost is relatively low, wherein woven cloth can be selected for the Polypropylence Sheet of polypropylene woven, nylon wire can be selected for sea fishing net, being made into 3 strands of a diameter of 0.08mm polyvinyl chloride wires, mesh is about 3 × 3mm (about 70 mesh).
Further, as preferably, described isolated material is the wire gauze of 70～75 mesh meshes, uses wire gauze, and the parasitic effects of dastarcus helophoroides is preferable, and isolation effect is preferable so that the pinaster Senescence rate around rickle is relatively low.
As preferably, a length of the 1～2m of described pine epidemic disease wood, a diameter of 4～12cm.
Pine epidemic disease wood state when entering heap can have various ways, as pine epidemic disease wood with whole form enter heap or from the lumbering face of pine epidemic disease wood length direction along pine epidemic disease pine epidemic disease wood is divided into multiple pine epidemic disease wooden unit, enter heap with the form of loose epidemic disease wooden unit.
As preferably, from the lumbering face of described pine epidemic disease wood, along ten word directions, pine epidemic disease is divided into 4 loose epidemic disease wooden units, four loose epidemic disease blocks will be processed into by every loose epidemic disease wood so that the plumage moth rate of Monochamus alternatus Hope is relatively low, and the parasitic rate of dastarcus helophoroides is higher, and prevention effect is preferable.
Described ovum is (10～40) piece with the quantitative proportion of pine epidemic disease wood moth hand-hole: 1, and described adult is (0.25～1) head with the quantitative proportion of pine epidemic disease wood moth hand-hole: 1.
Compared with prior art, it is an advantage of the current invention that: the present invention utilizes the parasite product of dastarcus helophoroides biological nature artificial breeding dastarcus helophoroides, cost is relatively low, and parasite quantity is guaranteed, epidemic prevention yard is then directly set in epidemic-stricken area and makes epidemic prevention yard be effectively isolated with the healthy pine strain of surrounding, to epidemic situation treatment in situ, epidemic-stricken area is become the place of a natural breed dastarcus helophoroides, greatly reduce the labor intensity of epidemic prevention, improve epidemic prevention efficiency, after dastarcus helophoroides is settled down, effect of preventing epidemic can be ensured for years, the preventing and treating that pine line is sick be applicable to Mountainous Area and forest needs.
Accompanying drawing explanation
Fig. 1 is ovum card acquisition process schematic diagram in the embodiment of the present invention；
Fig. 2 is dastarcus helophoroides inoculation and cocoon pupa adult output schematic diagram in the embodiment of the present invention；
Fig. 3 is that in the embodiment of the present invention, Monochamus alternatus Hope isolation escapes schematic diagram with dastarcus helophoroides；
Fig. 4 is that in the embodiment of the present invention, dastarcus helophoroides parasitism Monochamus alternatus Hope is affected by each test factor and test level；
Fig. 5 is that in the embodiment of the present invention, Monochamus alternatus Hope plumage moth rate and surrounding pinaster Senescence rate are affected by different closed materials.
Detailed description of the invention
The present invention is described in further detail by specific embodiment below in conjunction with accompanying drawing.
1, the acquisition of dastarcus helophoroides parasitism product
Select the natural enemy insect of a kind of day bovine trunk borer--dastarcus helophoroides (from forest-science academy of China), by learning to cultivate with Monochamus alternatus Hope larva for host, this dastarcus helophoroides is made to have height specialization (discernment is strong, and parasitic rate is high) to local Monochamus alternatus Hope.As shown in Figure 1, by arrow direction indication in Fig. 1, first take a bit of in have the Masson Pine section of Monochamus alternatus Hope larva, tie up fixing after wrapping up with multiwall kraft again with elastic, make a device luring collection dastarcus helophoroides to lay eggs, utilize dastarcus helophoroides to like the characteristic laid eggs around host's pine epidemic disease wood moth hand-hole, make dastarcus helophoroides in Corii Bovis seu Bubali laying eggs on paper, collect the ovum on kraft paper, obtain ovum card.
Take fraction ovum to be placed in cultivation box, after one week, ovum starts hatching for 1-dastarcus helophoroides in age larva, with brush pen picking 1-instar larvae, as shown in Figure 2, it is inoculated in the glass dactylethrae being loaded with Monochamus alternatus Hope maturation larva, finger-type mouth of pipe defat tampon blocks up, and the most not only can maintain certain humidity but also can prevent Monochamus alternatus Hope from runing away.After about spending 20 days, Monochamus alternatus Hope is by parasitic dead, and dastarcus helophoroides larvae development is the pupa spun of weaving silk, and obtains cocoon pupa.Finally, cocoon pupa is just sprouted wings through 20～30 and dastarcus helophoroides imago, and adult is moved on in container, obtains adult.
2, the release of dastarcus helophoroides parasitism product
Loose epidemic disease wood in pine line disease epidemic-stricken area is cut down and is classified as rickle, every rickle comprises 4～6 loose epidemic disease wood, this rickle is distributed in pine line disease epidemic-stricken area, in between annual 3～April, ovum card or adult it is invested in rickle and isolates with isolated material parcel rickle, have on isolated material and can block Monochamus alternatus Hope and the freely through mesh of dastarcus helophoroides imago can be made, as shown in Figure 3.
The Masson Pine that pine nematode epidemic-stricken area is the most withered taken from by loose epidemic disease wood in the present embodiment, a length of 1～2m, a diameter of 4～12cm, there is the moth hand-hole of Monochamus alternatus Hope larva and there is no emergence hole, i.e. require Masson Pine takes containing sawing in morbidity forest land in the Monochamus alternatus Hope larva survived the winter, December or February next year.
In the present embodiment, isolated material is woven cloth or nylon wire or wire gauze, wherein woven cloth is the Polypropylence Sheet of polypropylene braiding, nylon wire is sea fishing net, it is made into 3 strands of diameter 0.08mm polyvinyl chloride lines, mesh is about 3 × 3mm (about 70 mesh), in wire gauze, iron wire diameter 0.04mm, mesh is 3 × 3mm (about 70 mesh).
In the present embodiment, the heap form that enters of pine epidemic disease wood includes: with whole form (hereinafter referred to as log), from the lumbering face of pine epidemic disease wood, pine epidemic disease is divided into two parts (hereinafter referred to as 2 open wood) by the length direction laterally along pine epidemic disease portion, along ten word directions, pine epidemic disease is divided into four parts (hereinafter referred to as 4 open wood) from the lumbering face that pine epidemic disease is wooden.
3, prevention effect factor of influence research
3.1 experimental plots are arranged
Will test epidemic-stricken area 40hm2Forest land is uniformly divided into 40 communities, 195 loose epidemic disease wood are divided into 13 rafts, numbered: A, B, C, D, E, F, G, H, I, J, K, L, M, 13 rickles, numbered: a, b, c, d, e, f, g, h, i, j, k, l, m, the yard of lower case numbering is the repetition of capitalization numbering yard, 10, raft every heap wood, 5, rickle every heap wood.24 heaps in 26 heaps are used for discharging dastarcus helophoroides imago or the test of ovum card, and 2 heaps (M and n) are with comparing, and every heap is placed in test forest land, epidemic-stricken area randomly, and between heap and the heap of pine epidemic disease wood, unsoundness pinaster is separated by.Measurement, for trying diameter and the length of pine epidemic disease wood, counts every and eats into into hole number for the loose epidemic disease wood on the loose epidemic disease wood of examination.After counting, then E, F, G, H, e, f, g, h, 8 heaps are longitudinally cut open, and i.e. every epidemic disease wood is longitudinally being divided into two, and i.e. 2 open；I, J, K, L, i, j, k, l, 8 heaps, every loose epidemic disease wood longitudinal one is divided into four, and i.e. 4 open.
3.2 test factors are arranged
Experimental evidence L12(21×35) orthogonal modes, arrange according to the 2 hydraulic test factors 1, i.e. the worm state factor: set ovum card and 2 levels of adult；Worm state Quantitative factor: by for examination pine epidemic disease wood eat into into hole number, basic, normal, high 3 test level are set, its worm's ovum be often eat into hand-hole 10 pieces, 20 pieces, 40 pieces, adult is 0.25,0.5,1；Discharge time factor: discharge respectively at March in year, April and May；Pine epidemic disease wood form factor: set log, half log, 4 open wood 3 levels；Discharge form factor: set single-point and put, put at random, uniformly put 3 levels；Put and post the first rear enclosed factor: set plastic woven cloth, fishing nylon net, 3 levels of wire gauze.These test factors and level, by L12(21×35) orthogonal modes configuration, obtain 12 field test combinations.
Often the combination of group test factor is as follows:
10 pieces of log single-points in May of Aa: ovum put woven bag envelope heap；40 pieces of Bb: ovum log puts nylon wire envelope heap April at random；Ee: ovum is opened for 20 piece 2 and is uniformly put woven bag envelope heap wooden April；Ff: ovum open for 40 piece 2 wood March single-point put wire gauze envelope heap；Ii: ovum is opened for 20 piece 4 and is put nylon wire envelope heap wooden May at random；Jj: ovum is opened for 10 piece 4 and is uniformly put wire gauze envelope heap wooden March.
Cc: adult 1 log uniformly puts nylon wire envelope heap March；0.5 log single-point in April of Dd: adult puts wire gauze envelope heap；Gg: adult 0.5 2 opens puts woven bag envelope heap wooden March at random；Hh: adult 0.25 2 opens uniformly puts nylon wire envelope heap wooden May；Kk: adult 14 opens wood single-point in May puts woven bag envelope heap；Ll: adult 0.25 4 opens puts wire gauze envelope heap wooden April at random.
Mm: do not put ovum card not adult not splitting and do not seal heap, as comparison.Mid or late August, counting, for Monochamus alternatus Hope emergence hole quantity on examination pine epidemic disease wood, calculates eclosion rate.
The 3.3 different isolated material isolation pine epidemic disease wood containment effect to pine nematode
As it is shown on figure 3, in above-mentioned experimental plot, to woven cloth, nylon wire and 3 kinds of isolated materials envelope heaps of wire gauze, the four limit soil compactions in aforementioned of the loose epidemic disease yard for trying.Meanwhile, in every heap surrounding markings health pinaster 15-20 strain, to by the end of October, the pinaster of labelling is checked withered quantity, calculates mortality rate.Make comparisons with the withered pinaster quantity around examination epidemic disease yard that supplies not making to seal heap, calculate prevention effect.
3.4 result of the test
Test is final, checks 24 heaps test pine epidemic diseases wood and the loose epidemic disease carpentery workshop of 2 heaps comparison and dissects (splitting checks each pine epidemic disease wood moth hand-hole), it is thus achieved that statistical data.As shown in table 1, discharging dastarcus helophoroides group average plumage moth rate is 20.48 ± 16.69%, minimum LL group, is 3.31 ± 1.62%, is up to AA group, reach 55.57 ± 10.21%, but ratio comparison 81.27 ± 10.65%, also reduces by 25.7%, after showing to discharge dastarcus helophoroides, Monochamus alternatus Hope major part in pine epidemic disease wood is by parasitic, and eclosion rate is decreased obviously.Corresponding with Monochamus alternatus Hope plumage moth rate is correction parasitic rate, the dastarcus helophoroides correction parasitic rate average out to 74.81 ± 20.05 to Monochamus alternatus Hope, and the highest LL group reaches 96.14 ± 2.44%, and minimum is AA group, is 31.62 ± 12.56%.After result above shows pine epidemic disease wood is discharged dastarcus helophoroides imago or ovum card, the Monochamus alternatus Adults sprouted wings in pine epidemic disease wood significantly reduces, plumage moth rate is the lowest, show that parasitic rate is the highest, prevention effect is the best, the result of the test of this test has exceeded the test expection that general natural enemy insect is parasitic to host, shows that dastarcus helophoroides preventing and treating Monochamus alternatus Hope has good prospect.
Table 1 discharges after dastarcus helophoroides imago and ovum card Monochamus alternatus Hope eclosion rate table in pine epidemic disease wood
Affecting dastarcus helophoroides to Monochamus alternatus Hope parasitic effects factor for inquiring into, each test factor together with the change curve superposition of test level, result is as shown in Figure 4, it can be seen that each factor and the influence degree of level and trend.In 2 test level of the parasite factor, adult has bigger impact to result: release ovum card group Monochamus alternatus Hope average correction parasitic rate is 66.4%, and interval is between 25.5%-92.2%；Discharging adult group, average correction parasitic rate is 83.2%, and interval is 69.6%-95.9%.3 levels of parasite quantity are less on result of the test correction parasitic rate impact: the amount of discharging is low: in the amount of discharging: the amount of discharging height=75.38%:74.1%:74.81%, without significant difference, shows as a horizontal line；Discharging the time, March: April: May=81.61%:72.53%:73.78%, March, dispensing effect was the best；Epidemic disease wood state, log: 2 open: 4 open=63.02%:78.59%:82.67%, and log group parasitic rate is slightly lower；Dispensing mode, single-point: random: uniformly=69.29%:75.58%:79.44%, single-point group parasitic rate is slightly lower；Isolated material, woven cloth: nylon wire: wire gauze=57.47%:79.01%:87.81%, wire gauze group parasitic rate is the highest.As seen from Figure 4, the result of 3 levels of the amount of discharging is in the average bit of 24 heap result of the test 20% plumage moth rates, i.e. parasitic rate reaches 80%, show that the dastarcus helophoroides burst size in this interval range has reached Monochamus alternatus Hope height parasitic rate effect, with the dastarcus helophoroides amount of discharging horizontal line, each level of other each test factor is divided into upper and lower two intervals, and upper district is less than the impact on result of the test of the lower district to result of the test impact；On result impact maximum is closure, discharge day enemy's rear area and add woven cloth and the extreme difference adding wire gauze reaches 25%, i.e. the parasitic rate of the latter increases by 25% than the former, and nylon wire is placed in the middle, show that the such chemical materials of woven cloth and nylon wire is unfriendly to dastarcus helophoroides, affect parasitic effects；Result is also had a significant effect by epidemic disease wood state, and log state dastarcus helophoroides parasitism Monochamus alternatus Hope difficulty, 4 open state are the easiest, and both are about 16% by extreme difference；Other 2 factors, dispensing mode and time of discharging are respectively less than worm state factor to the impact of result, and the extreme difference caused is less than 10%.Can show that optimal experimental factor is combined as from this suite line: dastarcus helophoroides imago in the 3-4 month and with wire gauze closing, the parasitic effects higher than 90% can be reached.
After woven cloth, nylon wire and wire gauze close pine epidemic disease wood, the pinaster Senescence rate all making surrounding the most significantly declines, wire gauze: nylon wire: woven bag: comparison=1:2.25:4:32, shows that the comparison woods pinaster Senescence rate being not closed is 32 times of surrounding's pinaster Senescence rate that wire gauze is closed.Statistical analysis, wire gauze, nylon wire, woven bag are u with the test value compareed respectivelyFerrum - Right=10.993, uBuddhist nun - Right=9.864, uCompile - Right=8.631 ＞ u0.00000001=5.731 all have pole significant difference, fully show just to efficiently control pine nematode by pine epidemic disease wood isolation；Wire gauze and the test value u of nylon wireFerrum - Buddhist nun=1.414 ＜ u0.09=1.695 without significant difference, it is u with the test value of woven clothFerrum - Compile=2.768 ＞ u0.01=2.576, there were significant differences, nylon wire and the test value u of woven clothBuddhist nun - Buddhist nun=1.459 ＜ u0.09=1.695 without significant difference, shows that the effect of 3 kinds of spacer wire gauze isolation Monochamus alternatus Hopes is the best, but this effect is not all and is determined by closed material, and Monochamus alternatus Hope plumage moth rate also functions to effect.As seen from Figure 5, the Monochamus alternatus Hope plumage moth rate of matched group is close with the pinaster Senescence rate of surrounding, by this standard, the Monochamus alternatus Hope plumage moth rate of ferrum net group is minimum, pinaster Senescence rate about also ought to be minimum, but why ferrum net group Monochamus alternatus Hope plumage moth rate is minimum, it is to have reached the highest parasitic rate to Monochamus alternatus Hope owing to discharging dastarcus helophoroides.In this point, discharge in woods dastarcus helophoroides and suitable isolation just can efficiently control pine nematode popular with endanger
Tests prove that, the method is extremely effective to preventing and controlling pine wilt disease, and oil firing not electricity consumption is not without medicine, easy to operate ecological, environmental protective, controls worm with worm, cures the disease with worm, has fabulous application.
Claims (7)
1. the biological control method that a pine line is sick, it is characterised in that comprise the following steps:
(1) take a bit of in have the Masson Pine of Monochamus alternatus Hope larva, carry out binding with multiwall kraft after wrapping up fixing, flower
First ovum in Corii Bovis seu Bubali laying eggs on paper, collection kraft paper posted by floss, obtains ovum card；
Take part ovum to be placed in cultivation box, be placed in the ovum on spicule picking ovum card and be loaded with Monochamus alternatus Hope maturation larva
In transparent tube, mouth of pipe defat tampon blocks up, and after one week, when dastarcus helophoroides egg hatching is 1-instar larvae, 1-instar larvae is quickly
Being familiar with the body odour of Monochamus alternatus Hope larva and to Monochamus alternatus Hope parasitism, now dastarcus helophoroides larva grows on Monochamus alternatus Hope body,
Cocooing until weaving silk, during pupa, obtaining cocoon pupa；
Cocoon pupa, through 20～30 days, turns into dastarcus helophoroides imago, is collected in container by adult, obtains adult.
(2) the loose epidemic disease wood felling in pine line disease epidemic-stricken area is classified as pine epidemic disease yard, every heap comprises 4～10 loose epidemic diseases wood,
This pine epidemic disease yard is distributed in pine line disease epidemic-stricken area, between annual 3～April, described ovum card or adult are invested in pine epidemic disease
Isolating in yard and with isolated material parcel pine epidemic disease yard, it is the most withered that pine line disease epidemic-stricken area taken from by described pine epidemic disease wood
Masson Pine, this pine epidemic disease wood has Monochamus alternatus Hope larva moth hand-hole and does not has emergence hole, described isolated material has and can block
Monochamus alternatus Hope and the freely through mesh of dastarcus helophoroides imago can be made.
2. the biological control method that pine line as claimed in claim 1 is sick, it is characterised in that: described isolated material is
Woven cloth or nylon wire or wire gauze.
3. the biological control method that pine line as claimed in claim 2 is sick, it is characterised in that: described isolated material is
The wire gauze of 70～75 mesh meshes.
4. the biological control method that pine line as claimed in claim 1 or 2 is sick, it is characterised in that: described pine epidemic disease wood
A length of 1～2m, a diameter of 4～12cm.
5. the biological control method that pine line as claimed in claim 4 is sick, it is characterised in that: described pine epidemic disease wood is with whole
Root form enter heap or from the lumbering face of pine epidemic disease wood length direction along pine epidemic disease pine epidemic disease wood is divided into multiple loose epidemic disease wooden unit,
Heap is entered with the form of pine epidemic disease wooden unit.
6. the biological control method that pine line as claimed in claim 5 is sick, it is characterised in that: from described pine epidemic disease wood
Along ten word directions, pine epidemic disease is divided into 4 loose epidemic disease wooden units on lumbering face.
7. the biological control method that pine line as claimed in claim 1 or 2 is sick, it is characterised in that: described ovum and pine
The quantitative proportion of brown longicorn beetle larva moth hand-hole is (10～40) piece: 1, described adult eats into the quantity of hand-hole with Monochamus alternatus Hope larva
Ratio is (0.25～1) head: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610168757.0A CN105830824A (en) | 2016-03-23 | 2016-03-23 | Biological control method for bursaphelenchus xylophilus |
Applications Claiming Priority (1)
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| CN110637661A (en) * | 2019-09-26 | 2020-01-03 | 深圳市名高科技有限公司 | Nematode epidemic wood protective net and protection method |
| CN110956096A (en) * | 2019-11-13 | 2020-04-03 | 北京农业智能装备技术研究中心 | Forestry infection epidemic wood clearing management method and system |
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