CN101683069A - Artificial propagated batocera lineolata dastarcus helophoroides and technology for preventing and treating batocera lineolata - Google Patents
Artificial propagated batocera lineolata dastarcus helophoroides and technology for preventing and treating batocera lineolata Download PDFInfo
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- CN101683069A CN101683069A CN200810222846A CN200810222846A CN101683069A CN 101683069 A CN101683069 A CN 101683069A CN 200810222846 A CN200810222846 A CN 200810222846A CN 200810222846 A CN200810222846 A CN 200810222846A CN 101683069 A CN101683069 A CN 101683069A
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Abstract
The invention discloses artificial propagated batocera lineolata dastarcus helophoroides and technology for preventing and treating batocera lineolara. In an environment with temperature of between 24and 26 DEG C and humidity of between 60 and 70 DEG C, adult batocera lineolata dastarcus helophoroides is propagated and is induced to spawn; and spawned larva of the batocera lineolata dastarcus helophoroides is inoculated to a substituted host to ensure that larva parasitically grows and propagates the adult batocera lineolata dastarcus helophoroides. When the artificially propagated batocera lineolata dastarcus helophoroides is used for preventing and treating the batocera lineolara, eggs or adult batocera lineolata dastarcus helophoroides can be released. Release in the larval phase and pupal phase of the batocera lineolara can effectively prevent and treat the batocera lineolara, and the dropping rate of larval population of longicorn after one-year release can reach over 90 percentwithout environmental pollution. The eggs should be noticed to be clamped close to defecation holes of the larva when the eggs of the batocera lineolata dastarcus helophoroides are released, and release of the adult should be carried out at dusk.
Description
Technical field
The present invention relates to the biological control technology of a kind of insect, relate in particular to a kind of artificial propagation informal voucher longicorn dastarcus helophoroides and be used to prevent and treat clouding informal voucher longicorn technology.
Background technology
Clouding informal voucher longicorn (Batocera horsfieldi) belongs to the coleoptera Cerambycidae, is China Central China fast-growing and high-yield plantation, shelter forest and the most serious trunk borer of northern walnut woods, also is a kind of of bodily form maximum in China's Cerambycidae, prevents and treats very difficult.This longicorn is generally taken place in willow (Populus spp.) woods in area, the middle and lower reach of Yangtze River, and harm is serious.The willow and the economic forest seeds walnut of main harm black poplar group.Hazard approach is mainly eaten into food timber and is got food at cambium with larva in trunk, eaten riddled with gaping woundsly by moth in the trunk when serious, during one week, the nutrient delivery of trees is organized and is promptly blocked and destroy around trunk when Niu Gongtong harm on a little previous day, killed trees cambium ring, causing the tree that is injured dead.General be injured its height of tree of tree, the diameter of a cross-section of a tree trunk 1.3 meters above the ground and growth in volume are also had a strong impact on.The rate of being injured of willow that seriously is injured and walnut standing forest trees usually reaches 100%.
In recent years, willow since breeding easily, characteristics such as growth is fast, material is good, become main reproducting tree species in lakeside area, southern Plain and pulpwood seeds, also be the masters of these the regional shelter forests kind of planting trees.Along with the expansion day by day of willow cultivated area, the harm of clouding informal voucher longicorn also is on the rise.Larva is bored the harm of moth trunk, usually causes trees windbreak, death and causes dockage, has a strong impact on the growth of willow and the development of forestry industry, is the crushing insect of industrial timberland of willow short period.
In recent ten years, clouding informal voucher longicorn is taken place very serious in walnut producing region, Taihang Mountain (Henan, Shanxi, Hebei) and walnut producing region, the Qinling Mountains (Shaanxi, Henan), is the formidable enemy that walnut is produced.Trunk and the bough of clouding informal voucher longicorn main harm walnut, larva is bored the moth tunnel at cambium and xylem, destroy tree body nutrient delivery tissue, gently then cause a little less than the tree vigo(u)r sorrow walnut underproduction, even total crop failure, weight person's trunk is eaten hollow and complete stool is withered, causing the walnut mortality in the full bearing period, is the crushing insect of walnut, has had a strong impact on shaking off poverty and setting out on the road to prosperity of vast hill farmer.
Clouding informal voucher longicorn accounts for more than 90% with the time of larva movable harm in trunk in life, and its concealed life style makes control very difficult.The why big generation of this longicorn, the reason that causes the order of severity at present like this mainly is because artificial large tracts of land is built pure forests such as willow, walnut, make that the foodstuff of longicorn is very abundant, thereby its population quantity increases sharply, and the generation of natural enemy often lags behind, its population quantity does not reach and suppresses the degree that longicorn is caused disaster, causes the wildness of longicorn to take place.
Existing Prevention Technique mainly is to prevent and treat by chemical control and other conventional control methods, weak effect not only, cost height, and contaminated environment and fruit.And the natural enemy that utilizes special parasitic clouding informal voucher roundheaded borer of occurring in nature and pupa carries out biological control, and not only preventive effect is good, can also reach long-term Sustainable Control effect, and cost is low, to environment and fruit safety.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can effectively prevent and treat clouding informal voucher longicorn and environmentally safe biological control new technology, mainly comprise artificial propagation informal voucher longicorn dastarcus helophoroides and be used to prevent and treat clouding informal voucher longicorn technology.
The objective of the invention is to be achieved through the following technical solutions:
The technology of artificial propagation informal voucher longicorn dastarcus helophoroides of the present invention comprises step:
At first, carrying out the raising of described informal voucher longicorn dastarcus helophoroides adult and bringing out lays eggs;
Then, carry out the hatching of ovum, hatch the larva of described informal voucher longicorn dastarcus helophoroides;
Afterwards, utilize alternative host to raise the larva of described informal voucher longicorn dastarcus helophoroides, described larva is inoculated in makes its parasitic growth on the described alternative pin main body, weaving silk after larva is aging spins, and pupates wherein, turns into adult subsequently;
Environmental temperature in the said process is that 25 ℃ ± 1 ℃, humidity are 65% ± 5%.
The technology of control clouding informal voucher longicorn of the present invention; use the informal voucher longicorn dastarcus helophoroides control clouding informal voucher longicorn of the technology breeding of above-mentioned artificial propagation informal voucher longicorn dastarcus helophoroides, described informal voucher longicorn dastarcus helophoroides is released on the tree that is injured in described clouding informal voucher longicorn harm district.
As seen from the above technical solution provided by the invention, artificial propagation informal voucher longicorn dastarcus helophoroides of the present invention and be used to prevent and treat clouding informal voucher longicorn technology, because in temperature is that 25 ℃ ± 1 ℃, humidity are in 65% ± 5% the environment, the raising and bringing out of at first carrying out the adult of informal voucher longicorn dastarcus helophoroides is laid eggs; Carry out the hatching of ovum then, hatch the larva of described informal voucher longicorn dastarcus helophoroides; Utilize alternative host to raise the larva of described informal voucher longicorn dastarcus helophoroides afterwards, described larva is inoculated in makes its parasitic growth on the described alternative pin main body, breed out the adult of natural enemy at last.The harm district that the ovum and the adult of informal voucher longicorn dastarcus helophoroides can be released to clouding informal voucher longicorn can effectively prevent and treat clouding informal voucher longicorn, and non-environmental-pollution.
Embodiment
The technology of artificial propagation informal voucher longicorn dastarcus helophoroides of the present invention, its preferable embodiment is to comprise step:
At first, the raising and bringing out of carrying out the adult of informal voucher longicorn dastarcus helophoroides is laid eggs; Carry out the hatching of ovum then, hatch the larva of informal voucher longicorn dastarcus helophoroides; Afterwards, utilize to substitute the host and raise the larva of described informal voucher longicorn dastarcus helophoroides, make the natural enemy adult of its parasitism, bud into stalwartness, used alternative host is the prepupa of a kind of coleoptera beetle, also can be with the larva of other insect.Environmental temperature in the captive breeding process can be 65% ± 5% for 25 ℃ ± 1 ℃, humidity, also can select other temperature and humidity as required for use.
Carry out informal voucher longicorn dastarcus helophoroides adult raising and bring out to lay eggs and specifically can comprise:
At first, in supporting the worm plastic casing, (need before using to be stained with 75% alcohol with absorbent cotton, to the disinfection of box inner surface, and then be stained with distilled water with absorbent cotton, wiping box inner surface), pin one deck medical disinfecting gauze at the bottom of box, put into then and support the worm wooden unit, 3～4 layers littler than wooden unit surface area brown paper is put in described foster worm wooden unit top, described brown paper loam cake one slide, and described slide, brown paper and wooden unit bundled, also be placed with drinking bowl and feed dish in the described foster worm plastic casing, also be placed with the kraft paper bag of both ends open in the described foster worm plastic casing.
Used feed can be artificial feed prepared, and it is as follows to fill a prescription:
Comprise in the described feed prepared of every 50g: bloom first larva (comprising part batocera horsfieldi larva) powder 42.1g, dried yeast powder 5g, sucrose 1.65g, poplar bark powder 1.25g, micro-2g; It is standby that institute's feed prepared is placed on preservation below 0 ℃.
The preparation method of supporting the worm wooden unit can be to send the timber of seeds to make the wooden unit that the length and width height is 8.6 * 4.1 * 2.1cm with fresh black poplar, and wooden unit is wrapped with nonwoven.Also can adopt the wooden unit of other seeds.
Then, in foster worm plastic casing, put into 40 adults of informal voucher longicorn dastarcus helophoroides, can put into 35～45, and cover lid, be drilled with the aperture that a plurality of diameters are 0.5～1.5mm on the lid; In the informal voucher longicorn dastarcus helophoroides adult, the quantity of female worm is greater than the quantity of male worm in the box.
The hatching of carrying out ovum specifically can comprise:
The brown paper of resting above the worm wooden unit that has pieces of an egg takes out, and is put in the plastic casing, covers lid, is drilled with a plurality of apertures on the lid, checked in a plastic casing whether the first-instar young that has hatched is arranged, and the first-instar young that hatches is collected in per 2 days.
The larva that utilizes alternative host to raise informal voucher longicorn dastarcus helophoroides specifically can comprise:
Get 1 alternative host (prepupa of a kind of beetle),, in a kind of antimicrobial fluid short of money, soak earlier, dry, be put into then in the transparent plastic dactylethrae of long 50mm, diameter 15mm in order to prevent courses of infection; Be stained with a distilled water with the fine, soft fur pen again,, pick the informal voucher longicorn dastarcus helophoroides larva (being generally 8) of some (can be 7～9), be put into gently on the alternative pin main body according to the size that substitutes the host; Pipe is put in the 70g kraft paper strip doubling that can be 30 * 15mm then with length and width, near alternative host; Use the absorbent cotton jam-pack mouth of pipe again.Plastic tube does not come off and is advisable the degree that absorbent cotton stops up to pinch absorbent cotton with hand.Cultivate at last, breed out healthy and strong informal voucher longicorn dastarcus helophoroides adult.
The technology of control clouding informal voucher longicorn of the present invention; its preferable embodiment is; use the informal voucher longicorn dastarcus helophoroides control clouding informal voucher longicorn of the captive breeding method breeding of above-mentioned informal voucher longicorn dastarcus helophoroides, informal voucher longicorn dastarcus helophoroides is released to the harm district of described clouding informal voucher longicorn.Can discharge the adult or the ovum of informal voucher longicorn dastarcus helophoroides, concrete grammar is:
With cultivate and the adult of the informal voucher longicorn dastarcus helophoroides of sprouting wings packs and refrigerates:
Adult after at first will sprouting wings is that 16 ℃～23 ℃, humidity are to raise in 65% ± 5% the environment in temperature, raising temperature can be 18 ± 2 ℃ or 21 ± 2 ℃ etc., raise after 4～6 days male worm and 15 mixing of female worm are put into the adult release bag, the bad border refrigeration of then the adult release bag being put into 5 ℃ ± 1 ℃ is standby.
The ovum of informal voucher longicorn dastarcus helophoroides is made the ovum card and is refrigerated standby:
At first product there are the ox-hide scraps of paper of informal voucher longicorn dastarcus helophoroides ovum to cut off by the fan-shaped natural piece piecemeal of pieces of an egg, the pieces of an egg paper that will shear then adheres to the double leaf shape paper inner face after the doubling, and described double leaf shape paper ordered with stapler altogether and at opening part, make described ovum card; The environment that described ovum card is put into 6 ± 1 ℃ is stored.
Discharge ovum and prevent and treat constantly, the ovum that the ovum of the informal voucher longicorn dastarcus helophoroides of above-mentioned storage is made sticks into row and discharges, and the ovum card can be followed closely the tight top, place, defecation hole of clouding informal voucher roundheaded borer on trunk with stapler, and avoid sunlight direct projection ovum card.
When discharging adult, behind the adult release bag tearing notch with above-mentioned storage,, adult directly is poured on butt, lets alone freely to crawl into trunk top or diffusion and creep near on every side on other tree according to burst size.Can be during release in 5～October, 18:00 carries out later in the afternoon of sunny weather.
The specific embodiment of the captive breeding method of informal voucher longicorn dastarcus helophoroides of the present invention comprises:
Step 1, carry out the artificial feeding of informal voucher longicorn dastarcus helophoroides, make this natural enemy can normal development and protect kind:
Concrete grammar is with plastic casing with cover, at the bottom of box, spread a rectangle medical disinfecting gauze earlier, being beneficial to adult creeps and stands up activity, and put into two foster worm wooden units and each one of paper bag, drinking bowl and the feed box of perching for natural enemy adult lucifuge, put into 40 of informal voucher longicorn dastarcus helophoroides adults then, bonnet lid is preserved (being equipped with the ventilation hole of 5 aperture 1～2mm on the plastic box cover); Insect box of cleaning in two days changes feed, and adding distil water places 18 ℃ ± 2 ℃, the environment of humidity 60% ± 5% to raise.If desired to the raising of adult with bring out and lay eggs, carry out informal voucher longicorn dastarcus helophoroides large scale artificial breeding, then carry out step 2.
Step 2, to the raising of adult with bring out and lay eggs, carry out informal voucher longicorn dastarcus helophoroides large scale artificial breeding, specifically comprise:
Step 2-1, support designing and producing of worm frame: informal voucher longicorn dastarcus helophoroides first incubates first-instar young, the power of creeping and to bore seam very capable, even lid is got very tight, they also can find the space and climb out of outside the box; Thereby, need to make particular design to supporting the worm frame, so that the larva that climbs on the frame is high-visible, be convenient to collect inoculation reproduction.For this reason, the blue tinted glass that need select suitable thickness (3～5mm is thick) for use is easy to find on the stronger glass plate of this colour contrast so that health is flaxen larva as the dividing plate of supporting on the worm frame.
Step 2-2, at first, at foster worm plastic casing (in length and width height=20 * 15 * 6cm), shop one deck medical disinfecting gauze at the bottom of box, being beneficial to adult creeps and stands up activity, (length and width thickness=8.6 * 4.1 * 2.1cm) are done and are brought out the usefulness of laying eggs, and the wooden unit top is put 3 layers and is cut into the common brown paper slightly littler than wooden unit surface area, lays eggs thereon for adult to put into two foster worm wooden units then, paper loam cake one slide is reined in slide, brown paper and juggle with rubber band subsequently and is fixed; Put into then for the little dactylethrae of adult drinking-water and make drinking bowl, interior Sheng distilled water, the mouth of pipe with sponge beyond the Great Wall, in case water flows out; Put into the small container of Sheng adult artificial feed again, informal voucher longicorn dastarcus helophoroides adult is used for feeding; The kraft paper bag that to put into length and width subsequently be 8.5 * 4.5cm (both ends open of bag is concealed rest daytime for adult); Insert 40 of informal voucher longicorn dastarcus helophoroides adults at last, each 20 of wherein female male worms cover plastic box cover (be drilled with 5 apertures on the lid be 1～2mm ventilation hole); So just made a small-sized informal voucher longicorn dastarcus helophoroides " receptacle ".Placing temperature then is that 25 ℃ ± 1 ℃, the environment of the constant temperature and humidity of humidity 65% ± 5% are raised.Add water and artificial feed every day, drink and get food for adult.Checked in two days once, take out the foster worm wooden unit that ovum is arranged, change to new foster worm wooden unit, and check whether to have in the on glass and insect box of supporting the worm frame and hatched the larva that gets out.If find to have larva, collect immediately and manually connect worm, and transfer to step 3, carry out the raising of larva.If do not need to carry out immediately the raising of larva, then carry out step 2-3.
The making of step 2-3, informal voucher longicorn dastarcus helophoroides ovum card, packing and refrigeration:
The first step; the brown paper that has pieces of an egg above the foster worm wooden unit that will take out from step 2-2 takes off; the ovum that has the female worm of informal voucher longicorn dastarcus helophoroides to be produced on it; the ovum grain number that is once produced as the female worm of this natural enemy of raising with the method one is first is 56～350 not to be waited, and the ovum grain is very little, and length is only about 1mm; be long oyster shape; single ovum naked eyes are difficult to see clearly, and the ovum grain is the block arrangement of fan-shaped individual layer, and the interconnective phenomenon of a plurality of pieces of an egg is arranged.
Second step, the pieces of an egg on the ox-hide scraps of paper of band ovum to be cut off by fan-shaped natural piece piecemeal, little being cut into contains the pieces of an egg paper of pieces of an egg, and big being cut into contains the pieces of an egg paper of 2～3 pieces of an egg, makes each pieces of an egg paper approximately comprise 150 ovum.
The 3rd step, earlier fabrication is square (4 * 4cm) with the doubling of rectangle brown paper, the pieces of an egg paper back side that will shear then is coated with an amount of glue and (notes not being coated with glue too much, in order to avoid glue is penetrated into the ovum grain from the pieces of an egg paper back side, or escape into from the ovum paper back side on the pieces of an egg and cause ovum not hatch), adhere to leaf central part in the square double leaf paper after the doubling; Then double leaf paper is followed closely a pin with stapler altogether and at opening part, promptly made ovum and discharged card.
In the 4th step, the ovum that makes is discharged card put into ovum card packing box (the length and width height is respectively 27,14, the carton box of 18cm, and 8 little compartments of interior branch are wherein respectively adorned 60 ovum cards in 6 compartments, respectively adorn 70 ovum cards in 2 compartments.Like this, every box can be adorned 500 ovum cards altogether), will fill the packing box of ovum release card then and put into 6 ± 1 ℃ refrigerator cryopreservation.
The breeding of the raising of step 3 informal voucher longicorn dastarcus helophoroides larva, adult, packing and technique of refrigeration:
The hatching of step 3-1 ovum: the brown paper that has pieces of an egg above the foster worm wooden unit that will take out from step 2-2 takes out, or with the taking-up of the release of the ovum among step 2-3 card, concentrate to be put in the plastic casing, (covering and be drilled with 2～5 apertures the ventilation hole that is 1～2mm) closes the lid; Be placed on 25 ℃ ± 1 ℃, in the environment of the constant temperature and humidity of humidity 65% ± 5%, checked in a plastic casing whether the larva that has hatched is arranged, and, collected in the larva raising process in per 2 days the first-instar young that hatches.
The raising of step 3-2 larva: the first-instar young to occurring in the on glass and insect box of supporting the worm frame manually connects worm immediately.Method is will substitute one of host (prepupa of a kind of coleoptera beetle) earlier to put into transparent plastic dactylethrae (finger-type pipe range 50mm, diameter 15mm), gently be stained with the informal voucher longicorn dastarcus helophoroides larva of hatching just with little writing brush then, be put into this prepupa body surface gently, connect 8 informal voucher longicorn dastarcus helophoroides larvas (can breed 6 dastarcus helophoroides adults at last) on the general host; Pipe is put in the 70g kraft paper strip doubling that with length and width is 30 * 15mm then, near alternative host, at last with the absorbent cotton mouth of pipe beyond the Great Wall.The dactylethrae of worm will be connected; per 4 is 1 bundle with the rubber band bundle, puts into plastic casing, need not add a cover; be put in the environment of 25 ℃ ± 2 ℃ of temperature, humidity 60% ± 5% and raise, generally after 35～40 days, can see informal voucher longicorn dastarcus helophoroides adult eclosion of new generation.
The packing of step 3-3 adult and cold a surname: the adult after will the sprouting wings raising program in 1 is set by step raised, and adult is fed artificial feed to supplement the nutrients.Raise and divide male and female after 5 days, with 7 male worms, 8 female worms are put into the adult release bag, nail on stapler then and seal, put into adult packing box (for the carton box of length and width height=27 * 14 * 5cm) at last sidewards, 5 little compartments of interior branch are wherein respectively adorned 40 adult release bags in section, every box is adorned 200 altogether), then the adult packing box is put into 5 ℃ ± 1 ℃ refrigerator and cooled and hidden standby.
The present invention uses the concrete enforcement of the method for informal voucher longicorn dastarcus helophoroides control clouding informal voucher longicorn, comprises two kinds of methods:
Method one discharges informal voucher longicorn dastarcus helophoroides ovum control clouding informal voucher longicorn.
The tree that is injured of finding the harm of clouding informal voucher longicorn earlier and having fresh worm excrement to discharge, determine clouding informal voucher roundheaded borer quantity (a general clouding informal voucher roundheaded borer has only a defecation hole) according to the quantity in defecation hole, determine the burst size of dastarcus helophoroides ovum according to longicorn quantity.Adopt inoculation formula method for releasing.Can in mid-May in every year to October, after roundheaded borer enters active stage, discharge the dastarcus helophoroides ovum by the burst size of each gallery ovum card of nail (about 50～100 ovum).Ovum discharges card should be near place, defecation hole nail, and the dastarcus helophoroides larva of being convenient to newly to hatch like this can be easy to enter the longicorn tunnel, finds the roundheaded borer parasitism.During release, find clouding informal voucher roundheaded borer defecation hole earlier, then the ovum card is followed closely on the bark above the defecation hole with stapler, attention is close to the bark nail with the ovum card-edge as far as possible, so that the immature dastarcus helophoroides larva that hatches can climb on the bark down smoothly, enter in the roundheaded borer tunnel by the defecation hole.
Method two discharges informal voucher longicorn dastarcus helophoroides adult control clouding informal voucher longicorn.
When discharging the dastarcus helophoroides adult, also adopt inoculation formula method for releasing, per 3 trees that are injured that the harm of clouding informal voucher roundheaded borer arranged discharge 15 adults (release bag, in 7 male worms are arranged, 8 female worms).Specific practice is: to discharging for the first time the still bigger standing forest of insect density behind the ovum card, and can be after discharging for the first time one month, discharge the burst sizes of 6～20 adults by every strain tree, after afternoon is during 18:00, concentrate to discharge the dastarcus helophoroides adult and replenish control.To tear mouth in the adult release bag during release, adult is directly got final product in the butt of the tree that is injured.Attention bans use of chemical insecticide after natural enemy discharges.Discharging adult must carry out to darkness between the lights, because the adult of dastarcus helophoroides is hiding by day and coming out at night property, stops daytime and have a rest in the sheltered situation, and in the dusk and the activity of coming out evening, mate and oviposit, very active.Therefore, discharge daytime and suffer the injury of other natural enemy easily behind the adult and reduce control efficiency, and should after at dusk, discharge according to the active behavior of natural enemy.
Below some operation and predicate thereof and instrument in the above steps are elaborated:
Insect box: promptly the length and width height is the transparent plastic box with cover of 20 * 15 * 6cm, needs to dip in 75% alcohol with absorbent cotton before using, and its box inner surface is carried out wiping, disinfection.
Support the worm wooden unit: promptly send willow timber to make () wooden unit that the length and width height is 8.6 * 4.1 * 2.1cm, and wooden unit is wrapped with nonwoven with fresh black poplar.Concrete grammar is: with paper knife nonwoven is cut into the haik sheet of 11 * 26cm, foster worm wooden unit of every cloth sheet bag.The one side that glue will be arranged is promptly smeared with hand and is felt coarse one facing to wooden unit, rolls, and is transferred to proper temperature with electric iron then and presses, with two flatiron of wooden unit, nonwoven firmly is bonded on the wooden unit again.Support worm wooden unit top and put 3 greatly for a short time ox-hide scraps of paper that are respectively length and width 90 * 45mm, 80 * 40mm and 78 * 32mm (maximum below, minimum in the above), lay eggs for the dastarcus helophoroides adult, (length and width thickness=76 * 25 * 2mm) are bundled into one with rubber band with slide, brown paper and wooden unit to bonnet 1 slide then on the paper.
Informal voucher longicorn dastarcus helophoroides adult rest paper bag: because its adult active behavior is hiding by day and coming out at night property; therefore, with the brown paper of length and width 85 * 45cm size, peg outer rim with stapler after the doubling; both ends open allows lucifuge " rest " therein on informal voucher longicorn dastarcus helophoroides adult daytime.
Drinking bowl: with 2 long 40mm, aperture is that the transparent plastic dactylethrae of 13mm is made, and contains distilled water in the pipe, and the mouth of pipe with sponge or absorbent cotton beyond the Great Wall, and is but can not Sai Detai tight, so that water is permeable to sponge or absorbent cotton, is convenient to adult and drinks.At last two effective rubber bands are lashed, put into insect box.
The feed ware of feeding informal voucher longicorn dastarcus helophoroides adult: the vinyl disc of diameter 30mm, high 4mm.
Change feed: after informal voucher longicorn dastarcus helophoroides is eaten up artificial feed, need to add new feed; Do not eat up, normal as the feed color and luster, and do not have contaminatedly, just do not need to add again new feedstuff; Do not eat up, but the feed bad colour is normal, shows contaminatedly, just must swap out, put into new feed box and add new feed together with the feed ware.
The artificial feed of adult: i.e. the artificial feed of our development, be placed on after preparing below 0 ℃ preserve standby.
Manually connect worm: during artificial propagation informal voucher longicorn dastarcus helophoroides adult, the informal voucher longicorn dastarcus helophoroides first-instar young that has just hatched be received on the alternative pin main body,, be grown to adult then to help its parasitism.Method is: get 1 alternative host prepupa; in a kind of antimicrobial fluid short of money, dip in earlier; blot bacterium liquid with soft paper then; put into the transparent plastic dactylethrae of long 50mm, diameter 15mm; according to the individual size of alternative host; dip in a little distilled water with the fine, soft fur pen, pick 8 of the informal voucher longicorn dastarcus helophoroides larvas of firm hatching more gently, be put into gently and substitute on host's health.After connecing worm, be that pipe is put in the 70g brown paper doubling of 30 * 15mm, near alternative host, so that informal voucher longicorn dastarcus helophoroides mature larva is got behind parasitism and eaten the host, weaves silk and adhere on it when doing cocoon with length and width; Use the absorbent cotton jam-pack mouth of pipe again, put into and raise its parasitic growth of chien shih.The dastarcus helophoroides larva just will substitute the host and get food totally after general 7 days, and pupate other the cocooing of its corpse.A common alternative host can breed out 6～8 informal voucher longicorn dastarcus helophoroides adults.
Substitute host's preparation: will substitute the transparent plastic dactylethrae (every pipe is put 1) that the host puts into long 50mm, diameter 15mm, will concentrate in the plastic casing of packing into the plastics dactylethrae of worm then, it is standby to place 0～5 ℃ of refrigerator to preserve.
Adult release bag: with the brown paper of long 85mm wide 60mm, be folded into the pouch of 65mm * 35mm, opening 5mm place is folded the one side of pegging opening earlier with stapler, behind 15 of the mallambyx raddei dastarcus helophoroides adults of packing into, the 5mm place of another side opening is folded peg again.
The present invention provides the new technology of a cover biological control clouding informal voucher longicorn for the main trunk borer of producing control clouding this timber forest of informal voucher longicorn and economic forest; comprise and discharge parasite in larval phase and pupa time---informal voucher longicorn dastarcus helophoroides; and the method for artificial mass rearing of this parasitic enemy insect and breeding, and discharge the fourth of the twelve Earthly Branches and the good release Prevention Technique that discharges adult.Design and produce out ovum and discharge card and adult release bag; Having worked out long-term (5～6 months) preserves and the hatching and the adult normal spawning propagation technique that do not influence ovum; Worked out the suitable time and the concrete release tech that discharge natural enemy, provide a cover effective technical measures for utilizing this natural enemy to carry out large area control clouding informal voucher longicorn.Control effect in control back is remarkable, has accomplished simultaneously free from environmental pollution, under the prerequisite of maintaining ecological balance and protection bio-diversity, realizes effectively and the constantly target of Pest Control.
The present invention can also be in adult stage of clouding informal voucher longicorn (Batocera horsfieldi) host plant that swallows at its extra-nutrition---on the multiflora rose (Rosa multiflora), the sprinkling environmentally safe apholates such as young urea (for a kind of insect growth regulator, IGR) that go out, behind the longicorn imago feeding, cause female adult worm ovary childrenization, or make the ovum can not normal development, thereby reduce its follow-on insect population quantity greatly.Because genital system prematurity did not still possess the ability that mating is laid eggs when clouding informal voucher longicorn adult had just sprouted wings, must get food through supplementing the nutrients, its genital system and ovum just can be reached maturity behind the accumulation nutriment, last output.Extra-nutrition is grown the normal growth of clouding informal voucher longicorn and is bred very important.Utilizing on apholate control clouding informal voucher longicorn adult, the significantly reduced basis of its population quantity, discharge parasite---informal voucher longicorn dastarcus helophoroides (ovum and adult) larval phase and the pupa time of longicorn, carry out biological control.Control back control effect highly significant, and free from environmental pollution, protect the ecological balance and bio-diversity, and can realize the prevention target of effective and Sustainable Control insect.
Informal voucher longicorn dastarcus helophoroides (Dastarcus sp.) belongs to coleoptera and posts first section (Bothrideridae), is the important parasite of clouding informal voucher longicorn (Batocera horsfieldi).The present invention is by protecting and utilize the natural enemy of clouding informal voucher longicorn; help natural enemy to increase and the recovery population quantity; reach suppressing fully and effectively to clouding informal voucher longicorn population; implementation is to utilize the biological control of natural enemy; can cooperate simultaneously measures such as spraying environmentally safe apholate, will reach effectively and the control efficiency of Sustainable Control clouding informal voucher longicorn.Discharge at woodland; control efficiency is remarkable; in Hubei, the result of study of Hunan willow pilot forest shows; discharging then; the parasitic rate of test region informal voucher longicorn dastarcus helophoroides just reaches 65%; the insect population rate of going down reached more than 95% in 1 year, had effectively controlled the harm of this great insect, many standing forests control be difficult in 1 year found the defecation hole of longicorn and roundheaded borer.The control efficiency of the clouding informal voucher longicorn of harm walnut being carried out in the Shanxi Province shows that also dastarcus helophoroides discharges the back 1 year insect population rate of going down and reaches more than 85%.
The above; only for the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, and anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (10)
1, a kind of technology of artificial propagation informal voucher longicorn dastarcus helophoroides is characterized in that, comprises step:
At first, carrying out the raising of described informal voucher longicorn dastarcus helophoroides adult and bringing out lays eggs;
Then, carry out the hatching of ovum, hatch the larva of described informal voucher longicorn dastarcus helophoroides;
Afterwards, utilize alternative host to raise the larva of described informal voucher longicorn dastarcus helophoroides, described larva is inoculated in makes its parasitic growth on the described alternative pin main body, weaving silk after larva is aging spins, and pupates wherein, turns into adult subsequently;
Environmental temperature in the said process is that 25 ℃ ± 1 ℃, humidity are 65% ± 5%.
2, according to the technology of claim 1 described artificial propagation informal voucher longicorn dastarcus helophoroides, it is characterized in that the raising of the adult of described dastarcus helophoroides and bring out to lay eggs and comprise:
Earlier dip in 75% alcohol with absorbent cotton, to supporting the inside and outside surperficial wiping of worm plastic casing, disinfection is dipped in distilled water with absorbent cotton again, scrub box inner surface, and at the bottom of box, spread one deck medical disinfecting gauze, put into then and support the worm wooden unit;
3 layers littler than wooden unit surface area brown paper is put in described foster worm wooden unit top, described brown paper loam cake one slide, and described slide, brown paper and foster worm wooden unit bundled, also be placed with drinking bowl and feed dish in the described foster worm plastic casing, be placed with the informal voucher longicorn dastarcus helophoroides artificial feed of configuration in the described feed dish, the kraft paper bag that also is placed with both ends open in the described foster worm plastic casing is perched for adult;
Then; in described foster worm plastic casing, insert 35～45 of the adults of described informal voucher longicorn dastarcus helophoroides, and cover lid, be drilled with the aperture that a plurality of diameters are 0.5～1.5mm on the described lid; in the adult of described informal voucher longicorn dastarcus helophoroides, the quantity of female worm is greater than the quantity of male worm.
3, according to the technology of claim 2 described artificial propagation informal voucher longicorn dastarcus helophoroides, it is characterized in that: described informal voucher longicorn dastarcus helophoroides artificial feed, it is as follows to fill a prescription:
Comprise in the described feed prepared of every 50g: bloom first larva dry powder 42.1g, dried yeast powder 5g, sucrose 1.65g, poplar bark powder 1.25g, micro-2g;
It is standby that described feed prepared is placed on preservation below 0 ℃.
4, according to the technology of claim 2 described artificial propagation informal voucher longicorn dastarcus helophoroides; it is characterized in that: the preparation method of described foster worm wooden unit is; send the seasoned wood of seeds to make the wooden unit that the length and width height is 8.6 * 4.1 * 2.1cm black poplar; and described wooden unit wrapped with nonwoven, in case dastarcus helophoroides produces ovum in the timber conduit.
5, according to the technology of claim 1 described artificial propagation informal voucher longicorn dastarcus helophoroides, it is characterized in that: described alternative host is the prepupa of a kind of coleoptera beetle.
6, according to the technology of claim 1 described artificial propagation informal voucher longicorn dastarcus helophoroides, it is characterized in that: described larva is inoculated on the described alternative pin main body, and method comprises:
Get 1 described alternative host, in antimicrobial fluid short of money, soak, dry then; be put in the transparent plastic dactylethrae of long 50mm, diameter 15mm; pick 7～9 described informal voucher longicorn dastarcus helophoroides larva of hatching just with the fine, soft fur pen, be put into gently on the alternative pin main body, use the absorbent cotton jam-pack mouth of pipe at last.
7, a kind of technology of preventing and treating clouding informal voucher longicorn; it is characterized in that; application rights requires the informal voucher longicorn dastarcus helophoroides control clouding informal voucher longicorn of the technology breeding of 1 to 6 each described artificial propagation informal voucher longicorn dastarcus helophoroides, described informal voucher longicorn dastarcus helophoroides is released on the tree that is injured in described clouding informal voucher longicorn harm district.
8, according to the technology of claim 7 described control clouding informal voucher longicorns; it is characterized in that; comprise that the ovum of described informal voucher longicorn dastarcus helophoroides is made ovum release sticks into row release; concrete grammar is that described ovum is discharged the tight top that card is ordered the defecation hole of roundheaded borer on the trunk of clouding informal voucher longicorn harm, and avoids sunlight direct projection ovum to discharge card.
9, the technical measures of control clouding informal voucher longicorn according to claim 8 is characterized in that, the preparation method that described ovum discharges card comprises:
At first the fan-shaped natural piece piecemeal of the pieces of an egg that will be produced by described informal voucher longicorn dastarcus helophoroides with the ox-hide scraps of paper of ovum is cut off, then described pieces of an egg are adhered to the double leaf shape paper inner face after the doubling, and described double leaf shape paper closed at opening part order with stapler, promptly make described ovum and discharge card;
Described ovum release is blocked the environment of putting into 6 ± 1 ℃ to be refrigerated standby.
10, the technology of control clouding informal voucher longicorn according to claim 7, it is characterized in that, comprising packs the adult of described informal voucher longicorn dastarcus helophoroides into stores in the adult release bag, discharge when to be prevented and treated, after during concrete operations described adult release bag being torn mouth, adult in the bag is poured on the butt of the tree that is injured, letting alone freely to crawl into trunk top or diffusion creeps near on every side on other tree, seek roundheaded borer defecation hole, lay eggs in its vicinity, dastarcus helophoroides larva after the hatching promptly pierces the roundheaded borer tunnel, the parasitic roundheaded borer of eliminating;
The time that discharges adult is in 5～October, selects the 18:00 in afternoon of sunny weather to carry out later on.
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| CN200810222846A CN101683069A (en) | 2008-09-22 | 2008-09-22 | Artificial propagated batocera lineolata dastarcus helophoroides and technology for preventing and treating batocera lineolata |
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| CN103828791A (en) * | 2014-01-17 | 2014-06-04 | 徐华潮 | Method for preventing and treating pecan batocera horsfieldi with dastarcus helophoroides |
| CN104026084A (en) * | 2014-06-30 | 2014-09-10 | 江苏省林业科学研究院 | Technology for promoting oviposition of dastarcus helophoroides imagoes |
| WO2015027659A1 (en) * | 2013-08-25 | 2015-03-05 | Li Jianqing | Device for observing feeding selection of batocera lineolata larvae in different host populations |
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| CN105918713A (en) * | 2016-05-16 | 2016-09-07 | 遵义市林业科学研究所 | Dastarcus helophoroides feed and preparation method thereof |
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| WO2015027659A1 (en) * | 2013-08-25 | 2015-03-05 | Li Jianqing | Device for observing feeding selection of batocera lineolata larvae in different host populations |
| CN103828791A (en) * | 2014-01-17 | 2014-06-04 | 徐华潮 | Method for preventing and treating pecan batocera horsfieldi with dastarcus helophoroides |
| CN105191864A (en) * | 2014-06-27 | 2015-12-30 | 夏振强 | Method for breeding clanis bilineata tsingtauica mell locust trees |
| CN105191864B (en) * | 2014-06-27 | 2018-05-18 | 淮海工学院 | A kind of method of acacia tree cultivation beans pellet |
| CN104026084A (en) * | 2014-06-30 | 2014-09-10 | 江苏省林业科学研究院 | Technology for promoting oviposition of dastarcus helophoroides imagoes |
| CN104026084B (en) * | 2014-06-30 | 2017-02-15 | 江苏省林业科学研究院 | Technology for promoting oviposition of dastarcus helophoroides imagoes |
| CN105918713A (en) * | 2016-05-16 | 2016-09-07 | 遵义市林业科学研究所 | Dastarcus helophoroides feed and preparation method thereof |
| CN112931412A (en) * | 2019-12-11 | 2021-06-11 | 福州虫林野趣文化创意有限公司 | Ornamental beetle breeding method |
| CN115119805A (en) * | 2022-06-01 | 2022-09-30 | 中国林业科学研究院森林生态环境与自然保护研究所 | Efficient breeding method for dastarcus helophoroides |
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