CN115119805A - Efficient breeding method for dastarcus helophoroides - Google Patents
Efficient breeding method for dastarcus helophoroides Download PDFInfo
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- CN115119805A CN115119805A CN202210616811.9A CN202210616811A CN115119805A CN 115119805 A CN115119805 A CN 115119805A CN 202210616811 A CN202210616811 A CN 202210616811A CN 115119805 A CN115119805 A CN 115119805A
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- 241000164724 Dastarcus helophoroides Species 0.000 title claims abstract description 141
- 230000001488 breeding effect Effects 0.000 claims abstract description 191
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- 238000012258 culturing Methods 0.000 claims description 18
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- 241000382353 Pupa Species 0.000 claims description 10
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- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 8
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a breeding method of dastarcus helophoroides, which is characterized in that the activity of breeding hosts is limited by using a dastarcus helophoroides breeding tube, the breeding tube carrying the hosts and the primarily hatched larvae of dastarcus helophoroides are placed in a closed breeding box together, the characteristic of parasitic hosts is effectively and independently searched by using dastarcus helophoroides, the breeding efficiency of dastarcus helophoroides is remarkably improved by combining with the control of a method in the breeding process, the breeding cost is reduced, the operation is simple, convenient and rapid, and the method can be applied to large-scale breeding production of dastarcus helophoroides.
Description
Technical Field
The invention relates to the technical field of biological control, in particular to a breeding method of dastarcus helophoroides.
Background
The forest diseases and insect pests seriously restrict and threaten the sustainable development of the forestry in China, further influence the consolidation and development of the ecological civilization construction result in China, and must pay high attention. At present, the occurrence and harm of forest diseases and insect pests in China mean the first crop of trunk borers, the concealment of the trunk borers causes the difficulty of the conventional prevention and control means to play the role, the forest diseases and insect pests are effectively prevented and controlled on the premise of protecting the environment and not polluting the environment, the healthy growth of the forest is ensured, and the biological prevention and control is the first choice and the best choice. The comprehensive control technology mainly based on natural enemy insect dastarcus helophoroides is utilized to successfully solve the problem of serious diseases and insect pests seriously harming the forest health of China, wherein the serious diseases and insect pests comprise anoplophora glabripennis harming the 'three-north' protection forest, monochamus alternatus hope spreading the pine wood nematode disease, monochamus castaneus harming the natural broad-leaved forest mainly based on oak trees and the like.
At present, three methods for breeding dastarcus helophoroides are summarized. The method comprises the steps of collecting the egg cards of the dastarcus helophoroides for artificial inoculation, sequentially inoculating the larvae of the dastarcus helophoroides which are hatched at the abdominal nodes of breeding hosts by using a writing brush after the eggs are hatched, placing the breeding hosts with the larvae of the dastarcus helophoroides in a transparent finger-shaped tube, plugging the tube orifice by using a cotton ball, filling the breeding hosts in a plastic feeding box, and culturing under proper conditions. An artificial feed, which is specifically disclosed in Chinese patent applications with application numbers of CN201610321127.2 and CN201210474799.9, is used for feeding dastarcus helophoroides larvae according to nutritional ingredients required by the development of the dastarcus helophoroides larvae. One is equipment assistance, which is specifically referred to as Chinese patent application with application number of CN201410367163.3, the dastarcus helophoroides breeding box consists of a box body, an egg carrying plate and a box cover, and the color is black; the inner part of the breeding box is in a grid shape, and the egg carrying plate is arranged above the grid in the box; the egg carrying plate is provided with a small hole; the box cover is provided with air holes. Barley pest or yellow meal worm pupae are placed in the grid of the breeding box, the egg carrying plate with the dastarcus helophoroides egg cards is placed on the grid, and the lid of the breeding box is covered. The grid of the box body is suitable for the size of a single-head alternative host, and the egg carrying plate is provided with evenly distributed holes and can ensure that the larvae of the dastarcus helophoroides can pass through smoothly; the box cover is provided with air holes and does not allow the dastarcus helophoroides larvae to pass through.
The three technologies have certain problems, specifically as follows: the artificial inoculation is time-consuming and labor-consuming, the labor cost is high, only a single head or a plurality of heads of the parasitic beetle larvae can be dipped by the writing brush each time, the single head of the host needs to be inoculated for a plurality of times, in addition, the host has a large moving space in the finger-shaped tube, and due to the resistance of the breeding host to the parasitic behavior of the parasitic beetle, the parasitic effect is not ideal due to the falling of the parasitic beetle larvae caused by twisting the body, and the breeding rate is low. The artificial feed has high requirements on professional technologies, partial feed components are difficult to obtain, and related feed formulas and feeding methods are not mature at present, the produced dastarcus helophoroides have good and irregular quality, the survival rate of the dastarcus helophoroides larvae eating on the artificial feed is low, and the egg laying amount of adults is not high. The method is characterized in that the hatched larvae of the dastarcus helophoroides and the breeding hosts are placed in the same closed space by utilizing the characteristic that the dastarcus helophoroides can independently search the hosts and complete parasitism. But also because the activity space of the breeding host is larger, the host is easy to resist the parasitism of the dastarcus helophoroides, and the larvae of the dastarcus helophoroides fall off by twisting the body, so that the parasitism rate is low, and the breeding effect is not ideal.
Disclosure of Invention
The invention provides a breeding method of dastarcus helophoroides, which is provided by the invention in view of the problems that the existing breeding method is low in parasitic rate and poor in breeding effect due to the fact that the host activity space is too large and the anti-parasitic behavior is easy to generate.
In order to achieve the above object, the present invention is achieved by the following means.
The invention provides a breeding method of a dastarcus helophoroides, which is characterized in that a dastarcus helophoroides breeding device is used for breeding the dastarcus helophoroides, the dastarcus helophoroides breeding device comprises a breeding box and a breeding pipe, wherein the breeding box is formed by a box top cover, a box body and a box bottom cover, and the breeding pipe is a bearing device of a dastarcus helophoroides breeding host; the breeding tube is provided with a hollowed-out structure while limiting the free movement of the host, the hollowed-out structure enables the early-hatched larvae of the dastarcus helophoroides to search the host through the hollowed-out part and complete parasitism, and meanwhile, the aged larvae of the dastarcus helophoroides can migrate from the breeding tube to the bottom cover of the box through the hollowed-out part to pupate; the breeding box body is provided with air holes, and the size of the breeding box body does not allow the dastarcus helophoroides larvae to pass through; the method comprises the following steps:
(1) placing the dastarcus helophoroides egg card to be hatched on a box bottom cover in the breeding box;
(2) selecting suitable host pupae;
(3) placing host pupa in breeding tube;
(4) inserting the breeding tube with the host pupa into a breeding box for culturing;
(5) collecting the eclosion dastarcus helophoroides imagoes.
During specific operation, the dastarcus helophoroides egg cards are uniformly laid on the bottom cover of the breeding box, and the quantity of the dastarcus helophoroides egg grains laid is determined according to the size of the breeding host type and the density of the breeding hosts in the breeding box.
Preferably, the dastarcus helophoroides egg card is a dastarcus helophoroides egg which is hatched within 24 hours; preferably, the host is a barley pest, a yellow mealworm, a horned beetle, a monochamus alternatus, a anoplophora glabripennis, a Liriomys granulatus, a celestial beetle, a white streak longicorn and a mountain longicorn; preferably, selecting breeding hosts pupated for 0-2 days and placing the breeding hosts in a breeding tube; preferably, host pupae are treated by adopting a disinfection solution soaking and clear water washing mode; preferably, the culture conditions in (5) are: culturing at 25-29 deg.C and humidity of 60-70% in dark; preferably, after culturing for 36-60 hours, for example 48 hours, the bottom cover of the case is opened, the unhatched dastarcus helophoroides eggs are removed, the top cover of the case is closed, and the case is cultured under the same conditions until the dastarcus helophoroides are eclosized.
In a specific embodiment, the host pupae are placed in a breeding tube after being disinfected, and disinfection adopts modes of disinfectant soaking and clear water washing.
In a preferred embodiment, a partition plate with a partition grid is arranged in the box body and movably arranged in the box body, and the breeding pipe is movably arranged and fixed on the partition grid; breed the pipe by pipe body, support column and base and constitute, the pipe main part and the support ring is located breed the upper portion of pipe, the support column and the base is located breed the lower part of pipe, between the support column and/or base central authorities all are hollow out construction for the first larva of hatching of dastarcus helophoroides can pass through the fretwork position is searched the host and is accomplished parasitizing, and the old and mature larva of dastarcus helophoroides accessible of while the fretwork position is followed and is bred the pipe and moved to pupation in the box bottom cover.
Further, the division space is determined according to the size of the breeding box, the size of the breeding box is determined by the size of the host body, and the device is provided with more than one partition plates with the division spaces.
In a specific embodiment, the number of the support columns is two to eight; the support column, the pipe body and the base are manufactured integrally; preferably, the number of the support columns is four, and an inverted flat rectangular pyramid structure is formed.
Preferably, the cross section of the tube body is oval, the size of the oval is determined according to the host body type, and the host can be placed in the breeding tube and cannot move freely without damage.
In a preferable mode, the air holes are uniformly distributed on the peripheral side wall of the box body, the shape of the air holes is circular, and the size of the circular hole is not allowed to allow the dastarcus helophoroides larvae to pass through; the air holes are uniformly formed around the box body.
In a more preferable mode, the box top cover, the box bottom cover and the box body are detachably connected with the partition plate, so that the adult dastarcus helophoroides can be conveniently collected and the box body can be conveniently cleaned, and the box top cover and the box bottom cover are the same in size and can be replaced mutually.
In another specific embodiment, the upper end of the breeding tube is provided with a detachable or integrally formed fixing ring, the breeding tube is clamped above a grid formed by the partition plate through the fixing ring, and the breeding tube is limited to move relatively in the breeding box through the matching of the fixing ring and the grid; preferably, the fixed ring is a raised structure which is partially or completely outward in the periphery of the upper end of the breeding tube, a concave platform structure matched with the grid is arranged on the grid, and the breeding tube is clamped and fixed by matching the concave platform structure and the fixed ring so as to limit relative movement.
The high-efficiency breeding method of the dastarcus helophoroides is provided by matching the breeding tube with the breeding box, and the parasitic capacity of the dastarcus helophoroides on the breeding host is enhanced by limiting the activity space of the host. Compared with the prior art, the method has the following advantages: firstly, shortening the breeding period: the breeding tube limits the free movement of the host, ensures that the dastarcus helophoroides larvae can be successfully parasitized in a short time when the hosts are searched, effectively avoids the action of the hosts against parasitization, shortens the time for the dastarcus helophoroides to successfully parasitize the hosts, and further shortens the whole breeding period; secondly, the quality requirement of the breeding hosts is reduced: the prior inoculation method can achieve higher parasitic effect only by breeding the host within 24h after pupation, the requirements of the dastarcus helophoroides larva on the state of the host are reduced after the host activity is limited by the breeding tube, the high parasitic success rate can be still kept for the host within 48h of pupation, and the host within one week of pupation still has better parasitic effect; thirdly, the breeding efficiency is improved: in the prior art, hatched dastarcus helophoroides larvae are manually inoculated to a host by a writing brush, the invention utilizes the characteristic that the dastarcus helophoroides autonomously search parasitic hosts, and can autonomously search for the hosts and complete the parasitic after the dastarcus helophoroides eggs are hatched, thereby greatly saving the labor cost and time and improving the breeding efficiency; in addition, before manual inoculation, single-head hosts are placed in a finger-shaped tube, the invention can realize that 100 hosts can be independently parasitized by the dastarcus helophoroides larvae at most in one box body, thereby obviously improving the inoculation operation efficiency; the invention can ensure that the dastarcus helophoroides in the box body can be actively transferred to the bottom of the box body to carry out cocoon eclosion after the development of the dastarcus helophoroides is finished, is convenient to collect, saves time and labor, and effectively improves the efficiency of collecting the dastarcus helophoroides adults.
More preferably, the breeding method takes the put dastarcus helophoroides egg cards after culturing for 36-60h, preferably 48h, so that the dastarcus helophoroides successfully parasitized in 48h in the breeding devices are ensured, the dastarcus helophoroides in one breeding device can relatively keep consistent in development, and the emergence period is more regular. Therefore, the development of the dastarcus helophoroides can be ensured to be more orderly, and the commercial production is facilitated (for eggs which are not hatched for 48 hours, the eggs can still be continuously utilized, and the breeding cost is saved). Furthermore, the egg cards are evenly and flatly laid on the box bottom cover of the breeding box, the probability that each head of the dastarcus helophoroides larva searches for the host is the same, and the number of the dastarcus helophoroides produced by a single host on average can be determined by controlling the number of the dastarcus helophoroides eggs placed in the breeding device.
In a word, the method can obviously improve the parasitic rate of the dastarcus helophoroides to the breeding hosts and the breeding quantity of the dastarcus helophoroides on a single host, obviously improve the breeding efficiency of the dastarcus helophoroides, reduce the breeding cost, is simple, convenient and quick to operate, and can be applied to large-scale breeding production of the dastarcus helophoroides.
Drawings
FIG. 1 is a schematic diagram of the structure of a breeding tube of the present invention.
FIG. 2 is a schematic diagram of the structure of the breeding box of the present invention.
FIG. 3 is a schematic diagram of the structure of the breeding tube inserted into the breeding box of the present invention.
FIG. 4 is a flow chart of the breeding of dastarcus helophoroides according to the present invention.
Description of reference numerals: 1-tube body, 2-fixing ring, 3-support column, 4-base, 5-box top cover, 6-box body, 7-partition plate, 8-air hole and 9-box bottom cover.
Detailed Description
The invention is described in further detail below with reference to the drawings and the detailed description.
The invention relates to a dastarcus helophoroides breeding device and a method for breeding dastarcus helophoroides by using the device. The main ideas of the present invention are described by examples, wherein example 1 mainly describes a dastarcus helophoroides breeding device specifically used in the present invention, example 2 mainly describes a dastarcus helophoroides breeding method, and experiments were performed by selecting different hosts in examples 1 to 3 to show that the breeding method using the device is feasible.
Example 1:
the device for breeding the dastarcus helophoroides specifically used in the invention is shown in fig. 1-3. Wherein, the breeding tube (figure 1) comprises a tube body 1, and a fixing ring 2 is arranged on the outer side of the top of the tube body; the upper part of the tube body is in an oblate column shape, and other suitable shapes can be selected at the upper part; the lower part of the tube body is in an inverted quadrangular pyramid shape, the hollow part is connected with the tube body and the base 4 through four support columns 3, of course, the lower part of the tube body can be in other support shapes such as circular, and the number of the support can be 3-8 according to the distance between the support bodies. The breeding tube is designed and manufactured according to the shape and the size of the breeding host, so that the breeding host cannot move freely in the tube body, and meanwhile, the host cannot be extruded and damaged; the top of the tube body is fixed to keep the tube body stable in the breeding box; the hollowed-out part between the lower part of the breeding pipe and the base is convenient for the larvae of dastarcus helophoroides to directly search the hosts and complete parasitism through the hollowed-out part, and meanwhile, the aged larvae of dastarcus helophoroides can also migrate to the bottom cover of the box from the hollowed-out part at the lower part of the breeding pipe to pupate, so that the imagoes of dastarcus helophoroides can be conveniently collected.
According to the structural design of the body shape of the breeding host, the activity space of the breeding host can be well limited when the breeding host is arranged in the breeding tube, and the phenomenon that the parasitic rate is low because the larvae of the dastarcus helophoroides fall off from the host due to the resistance of the host to the parasitic behavior (twisting of the polypide) is avoided; on the premise of ensuring that the movement of the host can be limited, the hollow structure is added on the breeding pipe device to increase the searching probability of the dastarcus helophoroides to the host. The grids in the box body of the breeding box are suitable for the size of a single breeding pipe, so that the larvae of the dastarcus helophoroides can independently search hosts in a closed space and complete parasitism.
The breeding box (figure 2) is composed of a box top cover 5, a box body 6, a partition plate 7 and a box bottom cover 9, air holes 8 are uniformly distributed on the periphery of the box body, the aperture size of each air hole does not allow the dastarcus helophoroides larvae to pass through, the air holes 8 are uniformly formed on the periphery of the box body 6, and the whole box body is opaque and white in color. The box top cover 5 is convenient for placing the breeding tube in the box top cover, and the air holes on the box body 6 ensure the air permeability of the device; the grid formed by the partition plate 7 can be replaced according to the size of the breeding pipe, in addition, the breeding pipe can be kept stable in the grid, and the space in the box body can be utilized to the maximum extent; the box bottom cover 9 provides convenience for collecting the dastarcus helophoroides adults and cleaning the box body; the box top cover 5 and the box bottom cover 9 are the same in size and can be mutually used, and the use efficiency is improved. The breeding tube is used independently, and certain effect of the invention can be achieved; as long as the free movement of the host can be limited, the breeding efficiency of the dastarcus helophoroides can be improved to a certain degree. The invention limits the idea of host activity and can be used for breeding various natural enemy insects.
The combination of the breeding tube and the box body (figure 3) can obviously improve the parasitic rate and the breeding rate of the dastarcus helophoroides to the host, realize the autonomous search and the parasitic of the dastarcus helophoroides larva in the box body and the host in the breeding tube, and simultaneously facilitate the collection of the dastarcus helophoroides imagoes. Breed the pipe in figure 3 and place the cutting grid in division board 7, through breeding 2 joints of solid fixed ring of pipe in the grid top that division board 7 formed, the length that solid fixed ring stretches out the pipe body is unsuitable too long, avoids the interference between the adjacent solid fixed ring. After the breeding is completed, the user can extract the whole breeding tube from the separation grid by extracting the fixing ring 2. Can set up on division board 7 with solid fixed ring 2 matched with concave station structure, realize breeding the pipe joint fixedly through this concave station structure and solid fixed ring 2 cooperation. The device effectively solves the problems of low parasitic rate, poor breeding effect, difficult collection of imagoes, high breeding cost and the like of the dastarcus helophoroides, and is suitable for mass production and application of the dastarcus helophoroides.
Example 2:
the efficient breeding method of dastarcus helophoroides (the flow is shown in figure 4) comprises the following steps: a. and laying the dastarcus helophoroides egg cards to be hatched on the bottom cover of the breeding box. The number of the eggs of the dastarcus helophoroides can be determined according to the size of the breeding host body and the density of the breeding hosts in the breeding box, more eggs of the dastarcus helophoroides can be laid when the breeding hosts with larger body types or the density is higher, and less eggs of the dastarcus helophoroides can be laid when the breeding hosts with smaller body types or the density is lower. b. Selecting suitable host pupa. Selecting breeding host pupae which are healthy and active within 2 days and have no wound on body surface, wherein the breeding host can be barley pest, yellow mealworm, horny beetle, monochamus alternatus hope, anoplophora glabripennis, longicorn beetle, anoplophora chinensis, white longicorn beetle and longicorn beetle. c. The selected hosts are processed. And soaking and disinfecting the selected host pupae by using a disinfectant and washing the host pupae by using clean water. d. And placing the processed host in a breeding tube. The disinfected hosts are respectively put into breeding according to the mode that the heads of the hosts face upwards and the abdomens of the hosts face downwards, and the abdomens of the hosts can be ensured to be exposed from the hollow parts of the breeding tubes. e. Inserting the breeding tube with the host into a breeding box for culturing. Placing breeding tubes containing hosts in batches or one by one in grids in a breeding box, fixing the breeding tubes in gaps of the grids through fixing rings 2, and culturing the breeding box in a dark environment with the temperature of 25-29 ℃ and the humidity of 60-70%, preferably 27 ℃, the humidity of 65% and the total darkness; f. and removing the dastarcus helophoroides egg cards after 48 hours of culture. After culturing for 48h, opening the bottom cover of the box and removing the unhatched dastarcus helophoroides egg cards; g. collecting the eclosion dastarcus helophoroides imagoes. After the dastarcus helophoroides are eclosized, the box bottom cover is opened to collect the adult dastarcus helophoroides.
Application example 1:
selecting 2000 eggs of dastarcus helophoroides to be hatched, and uniformly spreading egg paper with the dastarcus helophoroides eggs on a bottom cover of a breeding box; selecting 100 barley pupae within two days of pupation, sterilizing the pupae (100 times of new benzalkonium chloride disinfectant soaking for 30s, washing for 3 times by clear water and drying in the shade), respectively putting the sterilized barley pupae into breeding tubes, putting the breeding tubes filled with the barley pupae into a breeding box (capable of accommodating 100 breeding tubes simultaneously), covering a box cover, and culturing under the conditions of 27 ℃, 65% humidity and full darkness. And opening the bottom cover of the breeding box after 48 hours, taking away the unhatched dastarcus helophoroides egg paper, covering the box cover, and culturing under the conditions of 27 ℃ and 65% humidity in full darkness. After 40 days, the imagoes of the dastarcus helophoroides are sequentially eclosized, the box bottom cover is opened to collect the imagoes in time, and 610 cocoons are formed to obtain 596 surviving imagoes of the dastarcus helophoroides, wherein the parasitizing rate of the dastarcus helophoroides to barley pupas is 92%.
Application example 2:
selecting 2000 eggs to be hatched, and evenly spreading the egg paper with the eggs on the bottom cover of the breeding box; selecting 100 monochamus alternatus pupae within two days of pupation, sterilizing pupa bodies (100 times of new benzalkonium bromide disinfectant liquid is soaked for 30s, washing for 3 times and drying in the shade), respectively putting the sterilized monochamus alternatus pupae into breeding tubes, putting the breeding tubes filled with the monochamus alternatus pupae into a breeding box (capable of containing 100 breeding tubes simultaneously), covering a box cover, and culturing under the condition of 27 ℃, humidity of 65% and full darkness. Opening the bottom cover of the breeding box after 48h, taking the unhatched dastarcus helophoroides egg paper, covering the box cover, and culturing under the conditions of 27 ℃ and 65% humidity in full darkness. After 40 days, the imagoes of the dastarcus helophoroides are sequentially eclosized, the box bottom cover is opened to collect the imagoes in time, and the imagoes are cocooned to 752, so that 745 live imagoes of the dastarcus helophoroides are obtained, and the parasitic rate of the dastarcus helophoroides to the pupas of the monochamus alternatus hope is 97%.
Application example 3:
selecting 2000 eggs to be hatched, and evenly spreading the egg paper with the eggs on the bottom cover of the breeding box; selecting 100 anoplophora glabripennis pupae within two days of pupation, sterilizing pupa bodies (100 times of new benzalkonium chloride disinfectant is soaked for 30s, washing for 3 times, and drying in the shade), respectively putting the sterilized anoplophora glabripennis pupae into breeding tubes, putting the breeding tubes filled with the anoplophora glabripennis pupae into breeding boxes (capable of containing 100 breeding tubes simultaneously), covering the breeding boxes, and culturing under the conditions of 27 ℃, 65% humidity and full darkness. Opening the bottom cover of the breeding box after 48h, taking the unhatched dastarcus helophoroides egg paper, covering the box cover, and culturing under the conditions of 27 ℃ and 65% humidity in full darkness. After 40 days, the imagoes of the dastarcus helophoroides are eclosized in sequence, the box bottom cover is opened, the imagoes are collected in time, 834 cocoons are formed, 817 surviving dastarcus helophoroides imagoes are obtained, and the parasitic rate of the dastarcus helophoroides to the anoplophora glabripennis pupa is 95%.
The above-mentioned embodiments are provided for illustration only, and therefore should not be used to limit the scope of the invention, i.e. equivalent variations and modifications made according to the present invention and the content of the specification should be covered by the present invention.
Claims (10)
1. The breeding method of the dastarcus helophoroides is characterized in that a dastarcus helophoroides breeding device is used for breeding dastarcus helophoroides, and comprises a breeding box and a breeding pipe, wherein the breeding box consists of a box top cover (5), a box body (6) and a box bottom cover (9), and the breeding pipe is used for carrying a host of dastarcus helophoroides; the breeding tube is provided with a hollowed-out structure while limiting the free movement of the host, the hollowed-out structure enables the early-hatched larvae of the dastarcus helophoroides to search the host through the hollowed-out part and complete parasitism, and meanwhile, the aged larvae of the dastarcus helophoroides can migrate from the breeding tube to the bottom cover (9) of the box through the hollowed-out part to pupate; the box body (6) is provided with air holes (8) with the size not allowing the dastarcus helophoroides larvae to pass through; the breeding method comprises the following steps:
(1) placing the dastarcus helophoroides egg cards to be hatched on a box bottom cover (9) in the breeding box;
(2) selecting suitable host pupae;
(3) placing host pupa in breeding tube;
(4) placing the breeding tube with the host pupa into a breeding box for culturing;
(5) collecting the eclosion dastarcus helophoroides imagoes.
2. The method for breeding dastarcus helophoroides according to claim 1, wherein: the dastarcus helophoroides egg cards are uniformly laid on the bottom cover (9) of the box, and the quantity of the laid dastarcus helophoroides egg grains is determined according to the size of the breeding host type and the density of the breeding hosts in the breeding box.
3. The method for breeding dastarcus helophoroides according to claim 1, wherein: the eggs of dastarcus helophoroides used will hatch within 24 h.
4. The method for breeding dastarcus helophoroides according to claim 1, wherein: the hosts are barley pests, yellow mealworms, spotted-brown longicorn, monochamus alternatus, anoplophora glabripennis, longicorn, anoplophora chinensis, longicorn and longicorn; preferably, selecting breeding hosts pupated for 0-2 days and placing the breeding hosts in a breeding tube; preferably, host pupae are treated by adopting a disinfection solution soaking and clear water washing mode; preferably, the culture conditions in (4) are: culturing at 25-29 deg.C under 60-70% humidity in dark; preferably, after culturing for 36-60 hours in the step (4), opening the bottom cover of the breeding box, removing the unhatched dastarcus helophoroides eggs, covering the top cover (5) of the breeding box, and culturing under the same conditions until the dastarcus helophoroides are eclosized;
preferably, the host pupae are treated and then placed into a breeding tube, wherein the treatment is to disinfect the host pupae by adopting a disinfection solution soaking mode and a clean water washing mode.
5. The method for breeding dastarcus helophoroides according to any one of claims 1 to 4, wherein: a partition plate (7) with a partition grid is arranged in the box body and can be movably arranged in the box body (6), and the breeding pipe can be movably arranged and fixed on the partition grid; breed the pipe by managing body (1), support column (3) and base (4) and constitute, manage main part (1) and support ring (2) are located breed the upper portion of pipe, support column (3) and base (4) are located breed the lower part of pipe, all be hollow out construction between support column (3) and/or base (4) central authorities for the first larva of hatching of dastarcus helophoroides can pass through the fretwork position host is searched and parasitism is accomplished, and the old and mature larva accessible of dastarcus helophoroides simultaneously the fretwork position is migrated to from breeding the pipe the pupation on box bottom (9).
6. The method for breeding dastarcus helophoroides according to claim 5, wherein: the number of the support columns (3) is two to eight; the supporting column (3), the pipe body (1) and the base (4) are manufactured integrally; preferably, the number of the supporting columns (3) is four, and an inverted flat rectangular pyramid structure is formed.
7. The method for breeding dastarcus helophoroides according to claim 5, wherein: the cross section of the tube body (1) is oval, the size of the oval is determined according to the host body type, and the situation that the host is placed in the breeding tube, cannot move freely and cannot be damaged is guaranteed.
8. The method for breeding dastarcus helophoroides according to claim 5, wherein: the side walls of the periphery of the box body (6) are uniformly distributed with the air holes (8), the air holes (8) are circular, and the pore size of the air holes does not allow the dastarcus helophoroides larvae to pass through.
9. The method for breeding dastarcus helophoroides according to claim 5, wherein: the box top cover (5), the box bottom cover (9), the box body (2) and the partition plate (7) can be detachably connected, so that adult dastarcus helophoroides can be conveniently collected and the box body can be conveniently cleaned, and the box top cover (5) and the box bottom cover (9) have the same size and can be replaced mutually.
10. The method for breeding dastarcus helophoroides according to any one of claims 5 to 9, wherein: the upper end of the breeding pipe is provided with a detachable or integrally formed fixing ring (2), the breeding pipe is clamped above a grid formed by the partition plate (7) through the fixing ring (2), and the relative movement of the breeding pipe in the breeding box body is limited through the matching of the fixing ring (2) and the grid; preferably, the fixing ring (2) is a raised structure which is arranged on the periphery of the upper end of the breeding tube partially or completely outwards, a concave platform structure matched with the grid is arranged on the grid, and the breeding tube is clamped and fixed by matching the concave platform structure with the fixing ring (2).
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2580762Y (en) * | 2002-12-06 | 2003-10-22 | 陈红印 | Storing device of parasitic insect |
| JP2006230275A (en) * | 2005-02-24 | 2006-09-07 | Yashima Sangyo Kk | Material for controlling boring noxious insect and method for controlling the same |
| CN101053321A (en) * | 2007-05-30 | 2007-10-17 | 盛茂领 | Trunk insect device |
| CN101589696A (en) * | 2008-05-28 | 2009-12-02 | 中国林业科学研究院森林生态环境与保护研究所 | The artificial propagation grain is takeed on the longicorn dastarcus helophoroides and is prevented and treated rust grain shoulder longicorn technology |
| CN101683069A (en) * | 2008-09-22 | 2010-03-31 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial propagated batocera lineolata dastarcus helophoroides and technology for preventing and treating batocera lineolata |
| CN102960559A (en) * | 2012-11-21 | 2013-03-13 | 南京林业大学 | Feed for artificially raising Dastarcus helophoroides in large scale and matched breeding technology thereof |
| CN103110029A (en) * | 2013-03-20 | 2013-05-22 | 遵义市林业科学研究所 | Feed formulation for increasing egg laying amount of Dastarcus helophoroides adult |
| CN104145882A (en) * | 2014-07-30 | 2014-11-19 | 北京市园林科学研究院 | Breeding box and method for high-efficiency production of dastarcus helophoroides |
| CN107306897A (en) * | 2017-08-17 | 2017-11-03 | 上海交通大学 | A kind of device for limiting aphid scope of activities and the method for pinpointing captive breeding aphid |
| CN214385633U (en) * | 2020-12-30 | 2021-10-15 | 广东省科学院动物研究所 | Parasitic wasp feeding and collecting device |
-
2022
- 2022-06-01 CN CN202210616811.9A patent/CN115119805B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2580762Y (en) * | 2002-12-06 | 2003-10-22 | 陈红印 | Storing device of parasitic insect |
| JP2006230275A (en) * | 2005-02-24 | 2006-09-07 | Yashima Sangyo Kk | Material for controlling boring noxious insect and method for controlling the same |
| CN101053321A (en) * | 2007-05-30 | 2007-10-17 | 盛茂领 | Trunk insect device |
| CN101589696A (en) * | 2008-05-28 | 2009-12-02 | 中国林业科学研究院森林生态环境与保护研究所 | The artificial propagation grain is takeed on the longicorn dastarcus helophoroides and is prevented and treated rust grain shoulder longicorn technology |
| CN101683069A (en) * | 2008-09-22 | 2010-03-31 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial propagated batocera lineolata dastarcus helophoroides and technology for preventing and treating batocera lineolata |
| CN102960559A (en) * | 2012-11-21 | 2013-03-13 | 南京林业大学 | Feed for artificially raising Dastarcus helophoroides in large scale and matched breeding technology thereof |
| CN103110029A (en) * | 2013-03-20 | 2013-05-22 | 遵义市林业科学研究所 | Feed formulation for increasing egg laying amount of Dastarcus helophoroides adult |
| CN104145882A (en) * | 2014-07-30 | 2014-11-19 | 北京市园林科学研究院 | Breeding box and method for high-efficiency production of dastarcus helophoroides |
| CN107306897A (en) * | 2017-08-17 | 2017-11-03 | 上海交通大学 | A kind of device for limiting aphid scope of activities and the method for pinpointing captive breeding aphid |
| CN214385633U (en) * | 2020-12-30 | 2021-10-15 | 广东省科学院动物研究所 | Parasitic wasp feeding and collecting device |
Non-Patent Citations (3)
| Title |
|---|
| 崔永三;刘云鹏;宋玉双;徐福元;徐克勤;: "应用天敌昆虫防治松墨天牛试点试验" * |
| 张彦龙等: "松褐天牛肿腿蜂对寄主松褐天牛三龄幼虫的功能反应", 《昆虫学报》 * |
| 林克剑等: "B型烟粉虱成虫对五种寄主植物的取食和产卵行为", 《植物保护学报》 * |
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