CN213663191U - In vitro insect culture device - Google Patents

In vitro insect culture device Download PDF

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CN213663191U
CN213663191U CN202022644776.0U CN202022644776U CN213663191U CN 213663191 U CN213663191 U CN 213663191U CN 202022644776 U CN202022644776 U CN 202022644776U CN 213663191 U CN213663191 U CN 213663191U
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chamber
inner cylinder
cylinder chamber
supporting frame
chamber cover
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张芳
巨翠红
闵祥芬
隋书婷
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Qingdao Tengrunxiang Testing And Evaluation Co ltd
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Qingdao Tengrunxiang Testing And Evaluation Co ltd
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Abstract

The utility model relates to an in vitro insect culture device, which comprises an insect breeding cylinder and a base, wherein the insect breeding cylinder comprises an inner cylinder chamber and an outer cylinder chamber, the outer cylinder chamber is sleeved on the inner cylinder chamber, and the inner cylinder chamber is also provided with a chamber cover matched with the inner cylinder chamber; the inner cylinder chamber comprises an inner cylinder wall, a placing frame and a bottom net, the placing frame is positioned in the middle of the inner cylinder chamber and comprises a placing disc, a supporting frame and a suspension rope, the supporting frame comprises a transverse supporting frame and a vertical supporting frame, the placing disc is positioned in the vertical center of the supporting frame, and the suspension rope is connected with the tail end of each supporting frame; the chamber cover comprises a chamber cover net and a chamber cover opening, the chamber cover net is positioned in the center of the chamber cover, and the chamber cover opening is positioned at the leftmost side; the outer cylinder chamber is connected with the base through a pillar. The device of the utility model has reasonable space design and is beneficial to the growth and development of insects; a plurality of ventilating devices are arranged, so that ventilation is facilitated, and the influence of overlarge humidity on the growth of insects is prevented; convenient observation, convenient cleaning and disinfection, repeated use for many times, and reduced test cost.

Description

In vitro insect culture device
Technical Field
The utility model belongs to the technical field of indoor artificial feeding insect, concretely relates to separation insect culture apparatus.
Background
Flood, insect and drought disasters have been listed as 3 natural disasters since ancient times, and the grain loss caused by insect damage in China is 5-10% every year. The traditional chemical pesticide spraying is a main means for preventing and controlling insect pests, but also causes a series of problems of increase of pesticide resistance of pests, pesticide residue, environmental pollution and the like. The research aiming at pest control and high-efficiency drug screening is gradually developed, the artificial feeding of various pests is continuously developed, and a large amount of research materials with consistent development are provided for the pest control and high-efficiency drug screening research.
The indoor insect feeding can not only study the growth and development conditions of insects and determine various life indexes of the insects such as the life cycle, feeding outbreak period and the like, but also screen out efficient prevention and control agents through an indoor formula screening test; monitoring the resistance of insects to various agents, etc.
The artificial feeding is greatly influenced by the environment, and the growth and development of insects can be influenced by the conditions of the feeding room environment, the feeding temperature, the feeding humidity and the like; in addition, the survival rate of the insects is greatly influenced by selecting the host plants or feeds with different formulas. The temperature is too high or too low, which can affect the growth and development of insects. Too high temperature easily causes the rapid loss of food; too low a temperature affects the enzymatic reactions in the insect body and affects the normal growth of the insect. The humidity is the same as the temperature, the specific activity of the enzymes in the insect body can be directly influenced, and the specific activity of the enzymes in the insect body can be influenced when the humidity is too high or too low.
In the prior art, the conventional feeding method comprises an artificial or semi-synthetic artificial feed method, a living plant method, an isolated plant method and the like. The reported device for raising insects has the problems of complex structure, difficult operation, higher cost, incomplete disinfection, inconvenience for observation and the like, and can not be used for raising insects normally for the requirement of scientific research. Therefore, research and development of the feeding device which is simple in structure, convenient to operate, convenient to observe and sterilize and suitable for insect growth has important significance for researching the life habits of insects, efficient medicament screening and the like.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the defects of complicated structure, troublesome operation, incomplete disinfection, difficult observation and the like of the insect culture device in the prior art, the utility model provides an insect culture device in vitro can realize single-end or quantitative feeding of insects, and simple operation, disinfection are thorough, convenient observation and the like.
The utility model discloses a realize above-mentioned technical purpose, provide following technical scheme: an isolated insect culture device comprises an insect breeding cylinder and a base, wherein the insect breeding cylinder comprises an inner cylinder chamber and an outer cylinder chamber, the outer cylinder chamber is sleeved on the inner cylinder chamber, and the inner cylinder chamber is also provided with a chamber cover matched with the inner cylinder chamber;
the inner cylinder chamber comprises an inner cylinder wall, a placing frame and a bottom net, the placing frame is positioned in the middle of the inner cylinder chamber and comprises a placing disc, a supporting frame and a suspension rope, the supporting frame comprises a transverse supporting frame and a vertical supporting frame, the placing disc is positioned in the vertical center of the supporting frame, and the suspension rope is connected with the tail end of each supporting frame;
the chamber cover comprises a chamber cover net and a chamber cover opening, the chamber cover net is positioned in the center of the chamber cover, and the chamber cover opening is positioned at the leftmost side;
the base is a cube, 100 pillars with 10 x 10 are arranged at the upper part of the base, and the outer cylinder chamber is connected with the base through the pillars;
further, the placing disc is disc-shaped;
furthermore, the inner cylinder chamber is in an open state, and the chamber cover is connected with the inner cylinder chamber through a connecting belt;
furthermore, the connecting belt is divided into an upper connecting belt and a lower connecting belt, and a marking area is arranged on the lower connecting belt;
further, the bottom net and the chamber cover net are 200-mesh gauze nets;
furthermore, a plurality of air holes are formed in the inner cylinder wall;
furthermore, four rope outlets are formed in the uppermost end of the inner cylinder wall;
furthermore, blocking balls with the diameter larger than that of the rope outlet are arranged on the outer sides of the rope outlet respectively and connected with the suspension ropes;
further, the support is a rhomboid column;
further, the lowest end of the outer cylinder chamber is provided with a support post hole.
Compared with the prior art, the utility model discloses following beneficial effect has:
1. the space design is reasonable, and the growth and development of insects are facilitated;
2. a plurality of ventilating devices are arranged, so that ventilation is facilitated, and the influence of overlarge humidity on the growth of insects is prevented;
3. the bottom net is arranged to effectively separate insects from excrement of the insects, so that the feeding space is clean, observation and cleaning and disinfection are convenient;
4. the device has the advantages of simple overall structure, convenience in operation, capability of being assembled at will, reusability and reduction of test cost.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of a partial structure of the insect-raising pot of the present invention;
fig. 3 is a schematic view of a partial structure of the rack of the present invention;
in the figure: in the figure: 1-inner cylinder chamber, 11-placing frame, 111-placing disc, 112-transverse supporting frame, 113-vertical supporting frame, 114-suspension rope, 115-separation ball, 12-inner cylinder wall, 121-ventilation hole, 122-rope outlet, 13-bottom net, 2-outer cylinder chamber, 21-pillar hole, 3-chamber cover, 31-chamber cover net, 32-chamber cover opening, 33-upper connecting belt, 34-lower connecting belt, 35-marking area, 4-base and 41-pillar.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
Example 1
As shown in figure 1, the isolated insect culture device comprises an insect culturing cylinder and a base 4, wherein the insect culturing cylinder comprises an inner cylinder chamber 1 and an outer cylinder chamber 2, the outer cylinder chamber 2 is sleeved on the inner cylinder chamber 1, and the inner cylinder chamber 1 is also provided with a chamber cover 3 matched with the inner cylinder chamber 1.
The chamber lid 3 can be closed or opened as shown in fig. 2, and in the closed state, the chamber lid 3 semi-encloses the entire apparatus; in an open state, the chamber cover 3 is connected with the inner cylinder chamber 1 through a connecting belt, and the connecting belt connects the whole device into a whole. The chamber cover 3 is integrated with the inner barrel chamber 1, so that the problems that the chamber cover 3 is not matched with the inner barrel chamber 1, and the insect escapes or is not covered tightly due to the cover are avoided. In addition, the connecting belt is divided into an upper connecting belt 33 and a lower connecting belt 34, and a marking area 35 is arranged on the lower connecting belt 34, so that the chamber cover 3 can be conveniently opened or closed, the upper connecting belt 33 and the lower connecting belt 34 can place the marking area 35 in the middle, and the numbers in the marking area are not easy to wipe by the outside, so that disorder of the insect-raising cylinders is caused.
The inner chamber 1 comprises an inner chamber wall 12, a placing frame 11 and a bottom net 13, wherein a plurality of air holes 121 are formed in the inner chamber wall 12, and the phenomenon that the inner chamber wall generates water drops due to heat and moisture generated in the insect breeding process and influences the growth and development of insects is avoided. The uppermost end of the inner cylinder wall 12 is provided with four rope outlets 122, the outside of the rope outlets 122 is respectively provided with a separation ball 115 with a diameter larger than that of the rope outlets, one end of the suspension rope 114 is connected with the tail end of each support frame, and the other end of the suspension rope is connected with the separation ball 115 through the rope outlets.
Rack 11 is located 1 middle part of inner tube room, rack 11 is including placing dish 111, support frame, suspension rope 114, the support frame includes horizontal support frame 112 and vertical support frame 113, places dish 111 and is located the support frame vertical center, places dish 111 and is discoid, can prevent to put host plant tuber, blade, artificial feed etc. effectively separates the insect rather than the excrement, carries out reasonable division with the space, guarantees to raise the space cleanness. The bottom net 13 and the chamber cover net 31 are 200 meshes of gauze, so that ventilation is facilitated, and the influence on the growth of insects caused by overlarge humidity is prevented.
The chamber cover 3 comprises a chamber cover net 31 and a chamber cover opening 32, wherein the chamber cover net 31 is positioned in the center of the chamber cover 3, and the chamber cover opening 32 is positioned at the leftmost side; the base 4 is a cube, 100 pillars 41 with 10 x 10 are arranged at the upper part, and the pillars 41 are rhomboid pillars, so that the structure is a rhomboid pillar type, on one hand, the base can be in contact with the pillars 41 to ensure the stability of the whole insect-breeding cylinder, and the outer cylinder chamber 2 is ensured to be in contact with the pillars 41; on the other hand can guarantee through the breach that air current from top to bottom prevents that the inner wall from producing the water droplet. The outer cylindrical chamber 2 and the base 4 are connected by a strut 41. The lowest end of the outer cylinder chamber 2 is provided with a support post hole 21, and the circle of the support post hole 21 is a circumscribed circle of a triangular rhombus cross section.
Example 2
Thrips Life cycle Table determination
Preparation of the test: picking 100 pupas of two indoor and outdoor populations respectively, adding fresh kidney beans when the pupas grow into adults, placing the adult pupas in a glass bottle, allowing the adult pupas to lay eggs for 24 hours and replacing the adult pupas once until all adults die, placing the fresh kidney beans replaced every day in an independent glass dish, observing the kidney beans in each dish twice every day, and observing whether hatching exists on the kidney beans for later use.
The first device comprises: 100 nymphs of 1 year old which are just hatched are respectively picked and independently raised in the insect rearing cylinder in the embodiment, and the specific operation is as follows: placing kidney bean blocks of 1mm × 1mm × 0.2cm into a placing tray 111, picking up newly hatched 1-year-old nymphs onto the kidney bean blocks, putting down a suspension rope 114, fixing the position of the placing rack in an inner cylinder chamber 1 by using a blocking ball 115, recording specific marks in a marking area, covering a chamber cover 3 on the back, buckling an outer cylinder chamber 2, and fixing the outer cylinder chamber on a base 4 through a support column 41.
A second device: the method comprises the following steps of respectively picking 100 nymphs of 1 year old nymphs which are just hatched out and independently feeding the nymphs in a 1.5 ml plastic tube, putting kidney beans cut into 1mm multiplied by 0.2cm into the tube in advance, specifically placing the kidney beans with a brush pen on the thrips (the kidney beans are easy to shake at the bottom of the plastic tube and are not easy to place the kidney beans at a fixed position), completing placement, and placing the kidney beans in a tray (the plastic tube lies horizontally and in the tray) after covering a plastic cover.
The food material is 1mm × 1mm × 0.2cm kidney bean blocks, fresh kidney bean blocks are replaced every two days, when the edge part of kidney bean is cut, the gap is not needed, the length is about 3cm, thrips are prevented from entering the gap once, and the kidney bean blocks are placed in a constant temperature box (the temperature is 25 +/-0.5 ℃, the relative humidity is 55% -60%, the light-dark ratio is 16L:8D), and observed at 9 am and 3 pm every day.
The development and death were recorded.
Figure BDA0002780413050000031
Figure BDA0002780413050000041
The above-mentioned, only be the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims (10)

1. An isolated insect culture device is characterized by comprising an insect culturing cylinder and a base, wherein the insect culturing cylinder comprises an inner cylinder chamber and an outer cylinder chamber, the outer cylinder chamber is sleeved on the inner cylinder chamber, and the inner cylinder chamber is also provided with a chamber cover matched with the inner cylinder chamber;
the inner cylinder chamber comprises an inner cylinder wall, a placing frame and a bottom net, the placing frame is positioned in the middle of the inner cylinder chamber and comprises a placing disc, a supporting frame and a suspension rope, the supporting frame comprises a transverse supporting frame and a vertical supporting frame, the placing disc is positioned in the vertical center of the supporting frame, and the suspension rope is connected with the tail end of each supporting frame;
the chamber cover comprises a chamber cover net and a chamber cover opening, the chamber cover net is positioned in the center of the chamber cover, and the chamber cover opening is positioned at the leftmost side;
the base is the square, and upper portion is equipped with 100 pillars totally of 10, outer cylinder room is connected through the pillar with the base.
2. The isolated insect culture device of claim 1, wherein the tray is in the shape of a disk.
3. The isolated insect culture apparatus according to claim 1, wherein the inner cylindrical chamber is opened, and the chamber cover and the inner cylindrical chamber are connected by a connecting band.
4. The isolated insect culture apparatus according to claim 3, wherein the connecting band is divided into an upper connecting band and a lower connecting band, and the lower connecting band is provided with a marking area.
5. The isolated insect culture apparatus of claim 1, wherein the bottom mesh and the cover mesh are 200 mesh screens.
6. The isolated insect culture apparatus according to claim 1, wherein the inner cylindrical wall has a plurality of air holes.
7. The isolated insect culture apparatus according to claim 1, wherein four rope outlets are provided at the uppermost end of the inner cylindrical wall.
8. The isolated insect culture device according to claim 7, wherein blocking balls with a diameter larger than that of the rope outlet are respectively arranged outside the rope outlet, and the blocking balls are connected with the suspension rope.
9. The isolated insect culture apparatus of claim 1, wherein the support is a rhomboid.
10. The isolated insect culture apparatus of claim 1, wherein the outer cylindrical chamber is provided with a support hole at the lowest end.
CN202022644776.0U 2020-11-16 2020-11-16 In vitro insect culture device Active CN213663191U (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2610195A (en) * 2021-08-24 2023-03-01 The Worm House Ltd Insect enclosure

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2610195A (en) * 2021-08-24 2023-03-01 The Worm House Ltd Insect enclosure
GB2610195B (en) * 2021-08-24 2024-04-03 The Worm House Ltd Beetle enclosure

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