CN104206362A - Phytotron for feeding prodenia litura and indoor artificial breeding method - Google Patents
Phytotron for feeding prodenia litura and indoor artificial breeding method Download PDFInfo
- Publication number
- CN104206362A CN104206362A CN201410480893.4A CN201410480893A CN104206362A CN 104206362 A CN104206362 A CN 104206362A CN 201410480893 A CN201410480893 A CN 201410480893A CN 104206362 A CN104206362 A CN 104206362A
- Authority
- CN
- China
- Prior art keywords
- prodenia litura
- phytotron
- day
- feeding
- litura
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Mushroom Cultivation (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a phytotron for feeding prodenia litura and an indoor artificial breeding method. The method comprises the following steps: putting prodenia litura larvae into the phytotron under a specific condition; selecting natural hosts (tobacco leaf, Chinese cabbage and the like) to feed the prodenia litura larvae; changing fresh hosts everyday and removing faeces and rotten leaves; after the prodenia litura larvae pupate, centrally putting the prodenia litura into an insect rearing box; after adult emergence of the prodenia litura, pairing the prodenia litura according to the female-male ratio of 1 to 1, laying eggs and breeding population. According to the feeding method, the prodenia litura are fed in the manmade phytotron, the feeding technique is simple and easy to operate, low in feeding cost, and high in reproductive rate, and standard health test insects can be obtained in an indoor continuous feeding manner.
Description
Technical field
The present invention relates to a kind of prodenia litura and raise phytotron and indoor artificial propagation method, belong to the breeding technology field of insect.
Background technology
Prodenia litura
spodoptera liturafabricius is subordinate to Lepidoptera, Noctuidae, another name lotus Autographa spp, and popular name tiger at night, five pink bollworms, numb worm etc., be one of global Important Agricultural insect.This worm host plant kind is 389 kinds of 109 sections (mutation) nearly, have a liking for and eat the wild cabbage of causing harm
brassica oleracea, Chinese cabbage
brassica campestrisl.spp.
pekinensis(Lour.) Olsson, capsicum
capsicum annuumdeng vegetables and wheat
triticum aestivum, paddy rice
oryza sativadeng cereal crops, the tobacco of also can causing harm
nicotiana tabacumdeng economic crops.With larva food leaf, bud, flower and fruit, when serious, full field crop can be eaten up, to agricultural production, caused huge loss.
Prodenia litura still be take chemical control at present as main, conventional dose is anti-heterodyne not only, its larva has also generally produced resistance, add that this worm larva is often hidden in soil seam, in weeds clump, during dispenser, be difficult for contact polypide, chemical control simultaneously also easily causes Chinese cabbage, the quality of the economic crops such as the edible safety of the vegetables such as wild cabbage and tobacco changes, cause chemical control more and more to be challenged, for effectively controlling causing harm of prodenia litura, population biology to prodenia litura, the basic research such as genecology and pesticide resistance and improvement is very necessary, Given this, indoor a large amount of, long-term lasting breeding Spodoptera litura Fabricius is an important step for scientific experiment.
At present, about prodenia litura artificial feeding technology, substantially be all devoted to the development and utilization of artificial feed, research comparative maturity, but artificial feeding is filled a prescription, easily collecting is not neat, store constant, some composition is expensive, Spodoptera litura larvae has gluttony habit in addition, artificial feeding is difficult to meet indoor feeding needs, Lv Baoqian etc. (2013) carry out prodenia litura single head raising on dactylethrae or Tissue Culture Plate, but single head is raised labor intensive, and waste feed, survival rate is low, so it is very necessary to realize a kind of raising method in the healthy prodenia litura worm of indoor colony amount reproduction source.
Summary of the invention
The object of the invention is: for the upper defect of prior art, provide a kind of prodenia litura to raise phytotron and indoor artificial propagation method, for realizing the extensive, long-term of the healthy worm of prodenia litura source, continue to raise, to overcome the deficiencies in the prior art.
Technical scheme of the present invention
A prodenia litura indoor artificial propagation method, the method is interior raising in the controlled environment chamber, comprises the following steps:
A, phytotron are built: artificial climate chamber device is supported worm frame, ventilation fan, hen house lighting controller, LED fluorescent tube, electric heating installation using oil as medium and temperature and humidity controller, phytotron imposes a condition: periodicity of illumination L:D=14 h:10 h, temperature are that 23-33 ℃, RH are 65-85%;
B, larval phase raise: Spodoptera litura larvae is picked up from tobacco Experimental Base and as experimental population, be positioned over the larva insect box in phytotron, the maintenance humidity of regularly spraying water every day, humidity range is 65-85%, and feed with host, change fresh host (tobacco leaf, Chinese cabbage etc.) every day, the absorbent cotton moisturizing of host's petiole, removes ight soil and rotten blade every day;
C, pupa time: Spodoptera litura larvae is pupated after 2 d, concentrate on insect box soil layer surface by pupa, the moisturizing of regularly spraying water every day, and humidity range is 65-85%;
D, adult stage: after pupa is sprouted wings, adult is raised in plastic cup by 1:1 pairing, and the source that supplements the nutrients;
E, ovum phase: collect ovum grain every day and be placed in culture dish, use preservative film capping, to the blackening of ovum grain, remove preservative film, change host's blade into and collect newly hatched larvae.
In above-mentioned prodenia litura indoor artificial propagation method, described larva insect box length 30 cm, width 20 cm, height 30 cm; Insect box bottom needs to spread the about 3-5 cm of thick layer, through 10 orders sieve and ultraviolet disinfection after sandy soil layer, every box is supported 200 ~ 500 of worms, with 160 order gauzes, covers tightly.
In above-mentioned prodenia litura indoor artificial propagation method, be positioned over sandy soil top layer in insect box pupa time, spray deionized water moisturizing every day, every box 180-230 head.
In above-mentioned prodenia litura indoor artificial propagation method, described adult is that female-male proportion 1:1 pairing is placed in 700 mL disposal plastic cups, extra-nutrition source adopts in plastic cup bottom and puts into the rayon balls that is moistened with 10% hydromel, with 160 order gauze sealings, change rayon balls every day, prevent from rotting to become to turn sour affecting Adult supplement nutrient, adopt 160 order gauze cover mouths, 160 order gauzes are conveniently laid eggs and are collected ovum grain.
In above-mentioned prodenia litura indoor artificial propagation method, ovum grain is placed in the culture dish of diameter 10 cm that are covered with filter paper moisturizing, uses preservative film capping, pricks 10 ~ 20 apertures ventilated on preservative film, the ovum grain of the ovum of collecting for producing on the same day, each culture dish is placed 800-1200 grain ovum.
In above-mentioned prodenia litura indoor artificial propagation method, insect box and sandy soil must, more than ultraviolet disinfection 2 h, be convenient to pupate before using.
In above-mentioned prodenia litura indoor artificial propagation method, host needs with clear water, to clean and dry, petiole absorbent cotton moisturizing before nursing.
A kind of prodenia litura is raised phytotron, comprise chamber body, ventilation fan is arranged on the door top of chamber body, supporting worm frame is evenly distributed in the body of chamber, at every one deck of supporting worm frame, all install by insect box, LED fluorescent tube be arranged on support on worm frame and be positioned at corresponding insect box directly over, at the middle part of supporting worm frame, be all provided with temperature and humidity controller, electric heating installation using oil as medium and humidifier are arranged on the body bottom surface, chamber of supporting between worm frame, and LED fluorescent tube is connected with the hen house lighting controller that is arranged on foster worm frame bottom.
Above-mentioned prodenia litura is raised in phytotron, and described chamber body is square building type structure.
Above-mentioned prodenia litura is raised in phytotron, and described foster worm frame is at least 2 rows.
Owing to having adopted technique scheme, compared with prior art, the present invention raises phytotron by prodenia litura is set, and controls its illuminance, temperature and humidity simulating natural condition completely truly, guarantee that its natural conditions are suitable for prodenia litura growth, by above-mentioned procreation method, realize the quick survival and growth of prodenia litura, procreation method of the present invention is simple, feeding cost is low, survival rate is high, can continue to raise, and obtains and stablizes in a large number healthy prodenia litura worm source.
Accompanying drawing explanation
Accompanying drawing 1 is the front view that middle twill noctuid of the present invention is raised phytotron;
Accompanying drawing 2 is vertical views that middle twill noctuid of the present invention is raised phytotron.
Embodiment
Below in conjunction with accompanying drawing to the present invention with being described in further detail, but not as any limitation of the invention.
Embodiments of the invention:
1, the construction of phytotron
A kind of prodenia litura is raised phytotron, as shown in drawings, comprise chamber body 1, this chamber body 1 is square building type structure, ventilation fan 2 is arranged on door 10 tops of chamber body 1, supporting worm frame 3 is evenly distributed in chamber body 1, this foster worm frame 3 is at least 2 rows, at every one deck of supporting worm frame 3, all install by insect box 4, LED fluorescent tube 6 be arranged on support on worm frame 3 and be positioned at corresponding insect box 4 directly over, at the middle part of supporting worm frame 3, be all provided with temperature and humidity controller 9, electric heating installation using oil as medium 7 and humidifier 8 are arranged on chamber body 1 bottom surface of supporting between worm frame 3, LED fluorescent tube 6 is connected with the hen house lighting controller 5 that is arranged on foster worm frame 3 bottoms.
Concrete construction step:
At window one jiao of the window in room (being chamber body) of (1.4 m * 1.4 m) of 4.2 m * 3.1, m * 3.6 m mono-sides, venetian blind type ventilation fan is installed, in room, by wall, place and raise frame (2 m * 1.8, m * 0.4 m) 3, raise three layers, frame, place insect box (30 cm * 30, cm * 20 cm) for every layer, 1 of the LED fluorescent tube of every layer of top suspension 12W of raising frame, tube power is controlled and (periodicity of illumination L:D=14 h:10 h is set by hen house illumination timing controller, daytime, 06:00 turned on light, night, 20:00 turned off the light), in room, place electric heating installation using oil as medium heating and temperature and humidity controller and control temperature in (28 ± 5) ℃, RH is (75 ± 10) %,
2, Spodoptera litura larvae is picked up from tobacco base, Lan Ba village, Majiang County, Guizhou Province, take back and indoorly set up experimental population in the controlled environment chamber, Spodoptera litura larvae is all raised in insect box (30 cm * 30, cm * 20 cm), be positioned in phytotron, insect box bottom paving thick layer approximately 3 cm, through 10 orders sieve and ultraviolet disinfection after sandy soil layer (be insect box and sandy soil layer all need sterilizing needs 2h more than), be convenient to pupate, every box is supported 200 ~ 500 of worms, with 160 order gauzes, cover tightly, the maintenance humidity of regularly spraying water every day, humidity is (75 ± 10) %, change fresh tobacco leaf every day, be host, tobacco leaf kind is river No. 3 (sample plot plantation), conventional variety south, remove every other day ight soil and rotten blade, host needs to clean and dry with clear water before nursing, petiole absorbent cotton moisturizing,
3, Spodoptera litura larvae pupates, after 2 d, pupa to be concentrated on to soil layer surface, sprays deionized water moisturizing every day, and humidity is (75 ± 10) %, every box 180-230 head;
4, after pupa is sprouted wings, adult is raised in 700 mL disposal plastic cups by 1:1 pairing, the rayon balls extra-nutrition that is moistened with 10% hydromel is put in plastic cup bottom, with 160 order gauze sealings, change cotton balls every day, change rayon balls every day, prevent from rotting to become to turn sour affecting Adult supplement nutrient, adopt 160 order gauze cover mouths, 160 order gauzes are conveniently laid eggs and are collected ovum grain;
5, collect the culture dish that ovum grain is placed in diameter 10 cm that are covered with filter paper moisturizing every day, with preservative film (pricking 10 ~ 20 apertures on preservative film) capping, to the blackening of ovum grain, remove preservative film, change host's blade into and collect newly hatched larvae;
Applicant uses the inventive method indoor lasting raising prodenia litura, and it on average goes through the phase in 33.33 about d, ovum phase wherein, larval phase, prepupal period, in pupa time, in the female adult stage, the male imago phase is on average respectively 3.14 d, 14.10 d, 1.43 d, 9.91 d, 4.74 d, 3.63 d.In 6 length of times of larval phase, on average go through each length of time is followed successively by 1.00 d phase, 1.33 d, and 1.40 d, 2.50 d, 3.27 d and 4.60 d, average survival rate is 95.32%; Pupa eclosion rate is 98.77%, the heavy 0.3041g of average pupa.Male and female are than average out to 0.92; 1353.20 of single female egg laying amount average out to.Compare existing artificial feed propagation technique (coroner is all auspicious etc., 2014; Lv Baoqian etc., 2013), cost of the present invention is lower, raises simply, raises polypide more healthy.
Claims (10)
1. a prodenia litura indoor artificial propagation method, is characterized in that, the method is interior raising in the controlled environment chamber, comprises the following steps:
A, phytotron build: periodicity of illumination L:D=14 h:10 h, temperature are that 23-33 ℃, RH are 65-85%;
B, larval phase, raise: Spodoptera litura larvae is picked up from tobacco Experimental Base and as experimental population, is positioned over the larva insect box in phytotron, the maintenance humidity of regularly spraying water every day, and humidity range is 65-85%, and feeds with host;
C, pupa time: Spodoptera litura larvae is pupated after 2 d, concentrate on insect box soil layer surface by pupa, the moisturizing of regularly spraying water every day, and humidity range is 65-85%;
D, adult stage: after pupa is sprouted wings, adult is raised in plastic cup by 1:1 pairing, and the source that supplements the nutrients;
E, ovum phase: collect ovum grain every day and be placed in culture dish, use preservative film capping, to the blackening of ovum grain, remove preservative film, change host's blade into and collect newly hatched larvae.
2. prodenia litura indoor artificial propagation method according to claim 1, is characterized in that: described larva insect box length 30 cm, width 20 cm, height 30 cm; Insect box bottom needs to spread the about 3-5 cm of thick layer, through 10 orders sieve and ultraviolet disinfection after sandy soil layer, every box is supported 200 ~ 500 of worms, with 160 order gauzes, covers tightly.
3. prodenia litura indoor artificial propagation method according to claim 1, is characterized in that: be positioned over sandy soil top layer in insect box pupa time, spray deionized water moisturizing every day, every box 180-230 head.
4. prodenia litura indoor artificial propagation method according to claim 1, it is characterized in that: described adult is that female-male proportion 1:1 pairing is placed in 700 mL disposal plastic cups, extra-nutrition source adopts in plastic cup bottom and puts into the rayon balls that is moistened with 10% hydromel, with 160 order gauze sealings, change rayon balls every day, prevent from rotting to become to turn sour affecting Adult supplement nutrient, adopt 160 order gauze cover mouths, 160 order gauzes are conveniently laid eggs and are collected ovum grain.
5. prodenia litura indoor artificial propagation method according to claim 1, it is characterized in that: ovum grain is placed in the culture dish of diameter 10 cm that are covered with filter paper moisturizing, use preservative film capping, on preservative film, prick 10 ~ 20 apertures ventilated, the ovum grain of the ovum of collecting for producing on the same day, each culture dish is placed 800-1200 grain ovum.
6. according to the prodenia litura indoor artificial propagation method described in claim 3 or 4, it is characterized in that: insect box and sandy soil must be more than ultraviolet disinfection 2 h before using.
7. prodenia litura indoor artificial propagation method according to claim 1, is characterized in that: host needs with clear water, to clean and dry, petiole absorbent cotton moisturizing before nursing.
8. a prodenia litura is raised phytotron, comprise chamber body (1), it is characterized in that: ventilation fan (2) is arranged on door (10) top of chamber body (1), supporting worm frame (3) is evenly distributed in chamber body (1), at every one deck of supporting worm frame (3), all install by insect box (4), LED fluorescent tube (6) be arranged on support worm frame (3) go up and be positioned at corresponding insect box (4) directly over, at the middle part of supporting worm frame (3), be all provided with temperature and humidity controller (9), electric heating installation using oil as medium (7) and humidifier (8) are arranged on chamber body (1) bottom surface of supporting between worm frame (3), LED fluorescent tube (6) is connected with the hen house lighting controller (5) that is arranged on foster worm frame (3) bottom.
9. prodenia litura according to claim 8 is raised phytotron, it is characterized in that: described chamber body (1) is square building type structure.
10. prodenia litura according to claim 8 is raised phytotron, it is characterized in that: described foster worm frame (3) is at least 2 rows.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410480893.4A CN104206362B (en) | 2014-09-19 | 2014-09-19 | Prodenia litura indoor artificial breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410480893.4A CN104206362B (en) | 2014-09-19 | 2014-09-19 | Prodenia litura indoor artificial breeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104206362A true CN104206362A (en) | 2014-12-17 |
| CN104206362B CN104206362B (en) | 2017-04-19 |
Family
ID=52088583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410480893.4A Expired - Fee Related CN104206362B (en) | 2014-09-19 | 2014-09-19 | Prodenia litura indoor artificial breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104206362B (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686473A (en) * | 2015-03-10 | 2015-06-10 | 中国农业科学院植物保护研究所 | Insect quantity survey method |
| CN104957398A (en) * | 2015-06-08 | 2015-10-07 | 云南大学 | Artificial feed for spodoptera litura larvae and raising method of spodoptera litura larvae |
| CN105028332A (en) * | 2015-06-10 | 2015-11-11 | 云南农业大学 | Method for shortening culture cycle of Argyrogramma agnata |
| CN105660539A (en) * | 2016-01-26 | 2016-06-15 | 贵阳学院 | Method for reproducing stegobium paniceum (L.) by utilizing semi-artificial feed |
| CN106857414A (en) * | 2017-04-06 | 2017-06-20 | 海南省农业科学院植物保护研究所 | The mass rearing method of prodenia litura |
| CN107242206A (en) * | 2017-07-17 | 2017-10-13 | 贵州大学 | A kind of ecological small-sized leafhopper breeding apparatus in imitative tea place |
| CN107410217A (en) * | 2017-05-16 | 2017-12-01 | 中国热带农业科学院环境与植物保护研究所 | The breeding apparatus and method of cassava monodactyle mite experimental population |
| CN107996525A (en) * | 2017-12-25 | 2018-05-08 | 贵州大学 | A kind of climatic chamber with air-flow regulatory function |
| CN108094331A (en) * | 2017-12-15 | 2018-06-01 | 广西壮族自治区农业科学院植物保护研究所 | A kind of narrow edge applies the indoor feeding method of noctuid |
| CN108684614A (en) * | 2018-04-27 | 2018-10-23 | 云南农业大学 | The device and application method for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process |
| CN110089642A (en) * | 2019-06-15 | 2019-08-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Spodopterafrugiperda man-made feeds |
| CN111133136A (en) * | 2017-09-27 | 2020-05-08 | 国立研究开发法人农业·食品产业技术综合研究机构 | Thread collecting device |
| CN112825817A (en) * | 2019-11-25 | 2021-05-25 | 北京大学 | Method for indoor feeding and breeding Kallima inachus under full-artificial condition |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09170A (en) * | 1995-04-20 | 1997-01-07 | Noyaku Bio Technol Kaihatsu Gijutsu Kenkyu Kumiai | Artificial feed for nami tentou |
| CN102113471A (en) * | 2011-02-22 | 2011-07-06 | 山东农业大学 | Artificial rearing method of larvae of beet armyworm |
| CN202276679U (en) * | 2011-10-12 | 2012-06-20 | 黄石市恒丰医疗器械有限公司 | Novel artificial climate chamber |
| CN103168753A (en) * | 2013-04-17 | 2013-06-26 | 香格里拉县宜康宝生物科技有限公司 | Method for culturing hepialus larvae indoors |
| CN203353474U (en) * | 2013-06-13 | 2013-12-25 | 天津市葱郁农业科技开发有限公司 | Breeding device suitable for black soldierflies |
| CN204180733U (en) * | 2014-09-19 | 2015-03-04 | 贵州大学 | A kind of insect raises phytotron |
-
2014
- 2014-09-19 CN CN201410480893.4A patent/CN104206362B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09170A (en) * | 1995-04-20 | 1997-01-07 | Noyaku Bio Technol Kaihatsu Gijutsu Kenkyu Kumiai | Artificial feed for nami tentou |
| CN102113471A (en) * | 2011-02-22 | 2011-07-06 | 山东农业大学 | Artificial rearing method of larvae of beet armyworm |
| CN202276679U (en) * | 2011-10-12 | 2012-06-20 | 黄石市恒丰医疗器械有限公司 | Novel artificial climate chamber |
| CN103168753A (en) * | 2013-04-17 | 2013-06-26 | 香格里拉县宜康宝生物科技有限公司 | Method for culturing hepialus larvae indoors |
| CN203353474U (en) * | 2013-06-13 | 2013-12-25 | 天津市葱郁农业科技开发有限公司 | Breeding device suitable for black soldierflies |
| CN204180733U (en) * | 2014-09-19 | 2015-03-04 | 贵州大学 | A kind of insect raises phytotron |
Non-Patent Citations (1)
| Title |
|---|
| 严珍 等: "取食番茄、辣椒和人工饲料对斜纹夜蛾生长发育和繁殖力的影响", 《热带作物学报》 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686473A (en) * | 2015-03-10 | 2015-06-10 | 中国农业科学院植物保护研究所 | Insect quantity survey method |
| CN104957398A (en) * | 2015-06-08 | 2015-10-07 | 云南大学 | Artificial feed for spodoptera litura larvae and raising method of spodoptera litura larvae |
| CN105028332A (en) * | 2015-06-10 | 2015-11-11 | 云南农业大学 | Method for shortening culture cycle of Argyrogramma agnata |
| CN105660539B (en) * | 2016-01-26 | 2018-06-15 | 贵阳学院 | Utilize the method for meridic diet procreation Stegobium paniceum |
| CN105660539A (en) * | 2016-01-26 | 2016-06-15 | 贵阳学院 | Method for reproducing stegobium paniceum (L.) by utilizing semi-artificial feed |
| CN106857414A (en) * | 2017-04-06 | 2017-06-20 | 海南省农业科学院植物保护研究所 | The mass rearing method of prodenia litura |
| CN107410217A (en) * | 2017-05-16 | 2017-12-01 | 中国热带农业科学院环境与植物保护研究所 | The breeding apparatus and method of cassava monodactyle mite experimental population |
| CN107242206A (en) * | 2017-07-17 | 2017-10-13 | 贵州大学 | A kind of ecological small-sized leafhopper breeding apparatus in imitative tea place |
| CN111133136A (en) * | 2017-09-27 | 2020-05-08 | 国立研究开发法人农业·食品产业技术综合研究机构 | Thread collecting device |
| CN108094331A (en) * | 2017-12-15 | 2018-06-01 | 广西壮族自治区农业科学院植物保护研究所 | A kind of narrow edge applies the indoor feeding method of noctuid |
| CN107996525A (en) * | 2017-12-25 | 2018-05-08 | 贵州大学 | A kind of climatic chamber with air-flow regulatory function |
| CN107996525B (en) * | 2017-12-25 | 2023-07-21 | 贵州大学 | Climate chamber with air flow adjusting function |
| CN108684614A (en) * | 2018-04-27 | 2018-10-23 | 云南农业大学 | The device and application method for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process |
| CN108684614B (en) * | 2018-04-27 | 2023-12-12 | 云南农业大学 | Device for preventing feed from mildew in frankliniella occidentalis feeding process and using method |
| CN110089642A (en) * | 2019-06-15 | 2019-08-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Spodopterafrugiperda man-made feeds |
| CN112825817A (en) * | 2019-11-25 | 2021-05-25 | 北京大学 | Method for indoor feeding and breeding Kallima inachus under full-artificial condition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104206362B (en) | 2017-04-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104206362B (en) | Prodenia litura indoor artificial breeding method | |
| CN102125002B (en) | Method for artificially raising plant bug parasitic wasps | |
| CN203897070U (en) | Indoor prodenia litura larva raising device | |
| CN102812923A (en) | Method for stocking high-quality broiler chickens in understorey purse net manner | |
| KR102013799B1 (en) | Cultivation method of mushrooms by supplying nutrient and controlling growth environment to need mushroom growth | |
| CN103535328B (en) | Method for industrially breeding phyoseiulus persimilis | |
| CN102870742A (en) | Large-scale artificial feeding method for eretmocerus hayati | |
| CN106900656B (en) | A kind of cigarette fleahopper industrial production method | |
| CN107372362A (en) | A kind of vermiculture bed for being easy to the later stage to separate | |
| CN106172244B (en) | Application of hermetia illucens adults as indicator organisms without pesticide in organic agriculture and breeding method thereof | |
| CN103907511A (en) | Radish seedling high-yield cultivation technology | |
| CN101803588B (en) | Method for breeding encarsia formosa all year round | |
| CN113519468A (en) | Method for feeding phyllotreta striolata in indoor generation | |
| CN106172248A (en) | A kind of expanding propagation method of Japan food a red-spotted lizard aphid chalcid fly | |
| WO2013082871A1 (en) | Three-dimensional combined-type culture bed having temperature and light controls | |
| CN104886009B (en) | A kind of moth class low instar larvae indoor culture method | |
| CN105379682B (en) | The extensive artificial fecundation method of aspongopus | |
| CN204180733U (en) | A kind of insect raises phytotron | |
| CN100548117C (en) | The scale artificial culture technology of white butterfly | |
| CN110024753A (en) | A kind of facility and method of anniversary Steady breed Encarsia formosa | |
| CN103907513A (en) | Alfalfa sprouting vegetable high-yield cultivation technology | |
| CN2242573Y (en) | Device for industrialized production of edible peanut sprouts | |
| CN110050762A (en) | A kind of method of hydroponics plant propagation aphid and food aphid cecidomyiia | |
| CN213663191U (en) | In vitro insect culture device | |
| CN211482530U (en) | Improve food replacement device of chilo suppressalis larva survival rate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170419 Termination date: 20200919 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |