CN102113471A - Artificial rearing method of larvae of beet armyworm - Google Patents
Artificial rearing method of larvae of beet armyworm Download PDFInfo
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- CN102113471A CN102113471A CN 201110044785 CN201110044785A CN102113471A CN 102113471 A CN102113471 A CN 102113471A CN 201110044785 CN201110044785 CN 201110044785 CN 201110044785 A CN201110044785 A CN 201110044785A CN 102113471 A CN102113471 A CN 102113471A
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Abstract
The invention discloses an artificial rearing method of larvae of beet armyworm, and the method comprises the following steps: (1) placing eggs of the beet armyworm in a ground glass hatching jar, placing fresh young cabbage leaves after surface disinfection by 75% of alcohol in the jar and sealing by using three-layer gauze till hatching; (2) selecting the freshly hatched larvae of the beet armyworm into a 24-hole insect rearing plate loaded with artificial feed, and rearing till 3 ages; and (3) transferring the 3-age larvae of the beet armyworm into flat bottom glass finger-shaped pipes loaded with the artificial feed, enabling each pipe to contain two larvae, and rearing till pupation. The method is simple, the rearing process occupies small space, the survival rate of the larvae is high, and a large number of standard test insects can be obtained.
Description
(1) technical field
The present invention relates to the insect breeding technology, be specifically related to the rearing method of beet exigua larvae.
(2) technical background
Beet armyworm (Spodoptera exigua) belongs to Lepidoptera, Noctuidae, is a kind of universal pest, and the plant that can cause harm is kind more than 170 nearly.Since the nineties in 20th century, all heavierly occurred in southern and northern each provinces and regions of China, the brassicaceous vegetable of mainly causing harm is also caused serious harm to shallot, asparagus, cotton, soybean, peanut etc.Beet armyworm happiness high temperature, it is mainly caused harm and focuses mostly on period megathermal period in various places.In China, with the rising of latitude, the year of beet armyworm algebraically takes place and reduce gradually.As northern areas 7～September (2～4 generation) such as Hebei, Henan, Beijing, Shandong, Anhui, Jiangsu; Shanghai, Hunan 6～October (3～5 generation) are caused harm heavier, and Guangdong and Fujian can take place in 1 year 8 generations, contain the phase of sending out in 5～September; Hainan, the torrid zone such as Shenzhen and part subtropical zone all can be bred the whole year, do not have the phenomenon of surviving the winter.Part area, China south, beet armyworm endangers throughout the year, does not survive the winter.Regional and the most of area, the north of China's south another part, the mixing worm source that enters to migrate from south is to replenish with the worm source of surviving the winter seldom, locality.Beet armyworm has produced the resistance of height to organic phosphates, carbamates and pyrethroid insecticides at present, add favourable pattern of farming and good growing weather, caused beet armyworm in recent years in the southern and northern generation of China situation in rising trend.Therefore beet armyworm being prevented and treated method and drug-fast research has become the problem of domestic and international concern, and raise by indoors artificial, obtain a large amount of subculture beet armyworm worms source, is the prerequisite of carrying out every research.
For effectively preventing and treating causing harm of beet armyworm, expansion such as biology, ecological property, chemical control technology and pesticide resistance research to beet armyworm is a basic work, and researchs such as the mechanism of action of the particularly medicament of beet armyworm screening, control medicament and pesticide resistance must have raised growth to grow consistent examination worm.Artificial feed and raising method are two key factors that insect is raised link.The research of beet armyworm artificial feed and rearing method is one of focus of research both at home and abroad always.The research and utilization artificial feed are sought method simple and easy, a large amount of, that subculture is raised beet armyworm, and well kind property is significant with the worm source that secures good health to population in the long-term holding chamber.The research of the beet armyworm artificial feed of open report at present is ripe, but in the research of beet armyworm rearing method at present, the raising method of the different worm attitudes of beet armyworm has nothing in common with each other, and also lacks a kind of easy raising method that a large amount of worms source can be provided.Li Guanghong (1998), Xu Jinhan (1998), Wu Xiaotian (1992), Mu Wei (2002), Goh (1991) and Wakanura (1988) etc. carried out research to indoors artificial feed and the raising method of beet armyworm, raising beet armyworm through indoor subculture found after 3～11 generations, survival rate of larvae, pupation rate, adult life-span, every female egg laying amount has obvious downward trend, can't raise by subculture.Li Tengwu (2001), Li Guanghong (1998) uses the plastic casing group support to the raising of larva, and packing is raised when growing to three ages.Mu Wei (2002), the flat glass tube single head of Song Yueqin uses such as (2009) raise beet exigua larvae to pupating, or the bull larva raising is in charge of raising after certain length of time and is treated that it pupates.Use 12 holes to support worm plate single head in the research such as Zhao Guoying (2010) and raise beet exigua larvae.
Beet exigua larvae is the main worm attitude of multinomial research, and therefore obtaining a large amount of healthy larvas is one of important process of beet armyworm raising.Above-mentioned work has solved in the research work difficult problem to beet armyworm standard worm source demand really to a certain extent, but simple and easy, a large amount of, subculture is raised beet armyworm and still existed one to be decided to be problem.(1) the larva survival rate is on the low side, and in the biologicall test of insecticide insect, the confidence level of result of study is not high.(2) pupation rate and pupa are heavy on the low side, influence the breeding of filial generation beet armyworm, and then influence quantity and the health degree of the examination worm of subculture raising.(3) raising method is loaded down with trivial details, and the effort of taking a lot of work is time-consuming.
(3) summary of the invention
The present invention at the weak point of above-mentioned existence, provides a kind of rearing method of beet exigua larvae based on existing research, thus realize simple and easy, obtain the purpose of beet exigua larvae in a large number.
The condition of artificial feeding beet armyworm is 26 ± 1 ℃ of temperature, and the photoperiod is 14h/10h (illumination/dark), and envionmental humidity is 60%～75%, and method step is as follows:
1. newly hatched larvae is raised: the beet armyworm ovum places glass hatching cylinder, places the tender cabbage leaves of the fresh children of a slice in the glass hatching cylinder, seals with 3 layers of gauze, treats its hatching; Ovum is used the clear water rinsing after 0.5% clorox soaks 10min, cabbage leaves is no pesticide pollution, and through mass fraction 75% ethanol surface sterilization.
2.1～3 instar larvaes are raised: newly hatched larvae is transferred to 24 holes with No. 0 writing brush supports in the worm plate (hole depth 20mm, aperture 15mm), the 1g artificial feed are equipped with in every hole, and every hole connects 4～6 of worms, and (body weight is about 10mg～12mg) to 3 ages in raising; Worm plate loam cake cushioning one deck blowing paper is supported in 24 holes, and (thick 2～3mm) to prevent the larva escape, raises to 3 ages; Worm plate and blowing paper are supported in 24 holes, and with mass fraction first bromine in 0.5% last of the ten Heavenly stems aqueous ammonium soaked overnight, flushing with clean water is dried the back and used.
3.3～5 instar larvaes are raised: 3 instar larvaes are changed in the flat glass dactylethrae (high 75mm, diameter 20mm), add the 3g artificial feed in the pipe, 2 of every pipes seal with aseptic tampon, raise until pupating; Flat glass dactylethrae with mass fraction first bromine in 0.5% last of the ten Heavenly stems aqueous ammonium soaked overnight, flushing with clean water, dries the back in 170 ℃ of hot air sterilizations 1 hour.
The invention has the beneficial effects as follows:
1. the present invention uses the tender cabbage leaves of fresh children to be put in the ovum hatching cylinder, has both kept humidity, can improve the incubation rate of ovum for newly hatched larvae provides foodstuff again, has overcome the high shortcoming of newly hatched larvae lethality.
2. beet exigua larvae raising method of the present invention, larva survival rate height, the examination of acquisition standard in a large number worm is realized the purpose of indoor a large amount of subculture raising beet armyworms.
3. beet exigua larvae raising method of the present invention is simple and easy, only need change feed one time in the whole feeding process of examination worm.
4. foster worm plate in 24 holes of the present invention's use and the equal economy of flat glass dactylethrae are easy to get, and it is little to take up room, reusable.
(4) embodiment
Below the specific embodiment of the present invention is further described.
Embodiment 1:2009 picks up from about 200 of the beet armyworm pieces of an egg in shallot field, Zhangqiu, Shandong, raises in indoor method of the present invention.
(1) ovum is placed in the hatching cylinder through sterilization, places fresh cabbage leaves in the cylinder.Final 153 of the newly hatched larvaes that obtain, incubation rate is 76.50%.
(2) get on the same day 96 of the newly hatched larvaes of hatching, insert 24 holes and support in the worm plate; 24 holes are supported in the worm plate artificial feed are housed, and 4 newly hatched larvaes in every hole are raised and obtained 92 of larvas to 3 ages, and 3 instar larvae survival rates are 95.83%.
(3) random choose beet armyworm 3 instar larvaes are 50, transfer in the flat glass dactylethrae, and artificial feed are housed in the dactylethrae, and 2 larvas of every pipe are raised to pupating, and the number of pupating is 47, and pupation rate is 94%, and on average pupa heavily is 112.5mg.
Raise the beet armyworm population that the field obtains with raising method of the present invention, can obtain desirable larva survival rate, the expansion that helps indoor population is numerous.
Embodiment 2: the beet armyworm ovum of getting subculture raising acquisition is an experiment material, raises by method of the present invention.If with reference to raising method: do not place the tender cabbage leaves of fresh children in the hatching process of ovum, newly hatched larvae is forwarded in the flat glass dactylethrae and raises to pupating the same the present invention of other details.Two kinds of raising method are all established 3 repetitions.
(1) raising method of the present invention: get on the same day pieces of an egg and be placed in the hatching cylinder through sterilization, place fresh cabbage leaves in the cylinder, statistics obtains the newly hatched larvae number, calculates incubation rate; Choose the newly hatched larvae of hatching on the same day, insert 24 holes and support in the worm plate, 24 holes are supported in the worm plate artificial feed are housed, and 4 newly hatched larvaes in every hole are added up 3 instar larvae numbers, calculate 3 instar larvae survival rates; Random choose beet armyworm 3 instar larvaes are transferred in the flat glass dactylethrae, and artificial feed are housed in the dactylethrae, and 2 larvas of every pipe are raised to pupating, adding up and pupate number with on average pupa is heavy, the calculating pupation rate.
The characteristic parameter that table 1 raising method of the present invention is raised beet armyworm ovum and larva
(2) with reference to raising method: get on the same day pieces of an egg and be placed in the hatching cylinder through sterilization, statistics obtains the newly hatched larvae number, calculates incubation rate; Choose the newly hatched larvae of hatching on the same day, insert in the flat glass dactylethrae, artificial feed are housed in the pipe, 1 newly hatched larvae in every hole is added up 3 instar larvae numbers, calculates 3 instar larvae survival rates; Beet armyworm 3 instar larvaes that the flat glass dactylethrae of random choose is raised are observed it and are pupated, and statistics is pupated number with on average pupa is heavy, calculates pupation rate.
Table 2 is raised the characteristic parameter of beet armyworm ovum and larva with reference to raising method
More than 2 contrasts that form is beet armyworm raising method of the present invention and a kind of raising method of having delivered, data as can be seen from table, the incubation rate of the ovum that raising method of the present invention obtains, larva survival rate, pupation rate and certain advantage is all arranged can be raised for the subculture of beet armyworm good basis is provided.And be compared to reference to raising method, it is little to take up room in raising method saving of work and time of the present invention and the feeding process, has improved the efficient that beet exigua larvae is raised.
Claims (4)
1. the rearing method of a beet exigua larvae is characterized in that: may further comprise the steps:
(1) with the ovum of beet armyworm, place glass hatching cylinder, place a slice cabbage leaves in the hatching cylinder, treat its hatching;
(2) the beet armyworm newly hatched larvae is inserted 24 holes that artificial feed are housed and support in the worm plate, every hole is inserted 4～6, supports cushioning one deck blowing paper in the worm plate lid, raises to 3 ages;
(3) beet armyworm 3 instar larvaes are transferred to the flat glass dactylethrae that artificial feed are housed, 2 of every pipes are raised to pupating.
2. according to claim 1, it is characterized in that: the described cabbage leaves of method step (1) wherein, do not use agricultural chemicals and behind mass fraction 75% ethanol disinfection, used.
3. according to claim 1, it is characterized in that: wherein worm plate and blowing paper are supported in described 24 holes of method step (2), and after mass fraction first bromine in 0.5% last of the ten Heavenly stems aqueous ammonium soaked overnight, flushing with clean water is dried the back and used.
4. according to claim 1, it is characterized in that: the described flat glass dactylethrae of method step (3) wherein, after mass fraction first bromine in 0.5% last of the ten Heavenly stems aqueous ammonium soaked overnight, flushing with clean water is dried the back in 170 ℃ of hot air sterilizations 1 hour.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104206362A (en) * | 2014-09-19 | 2014-12-17 | 贵州大学 | Phytotron for feeding prodenia litura and indoor artificial breeding method |
| CN104757333A (en) * | 2015-04-23 | 2015-07-08 | 中国农业科学院植物保护研究所 | Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method |
| CN105028332A (en) * | 2015-06-10 | 2015-11-11 | 云南农业大学 | Method for shortening culture cycle of Argyrogramma agnata |
| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN104186430B (en) * | 2014-08-14 | 2016-06-29 | 中国热带农业科学院椰子研究所 | The method of the laboratory test population mass rearing of moth knitted by Cortex cocois radicis |
| CN106259221A (en) * | 2016-08-19 | 2017-01-04 | 华巧波 | A kind of method raising beet armyworm |
| CN106306389A (en) * | 2016-08-19 | 2017-01-11 | 华巧波 | Fodder for improving breeding efficiency of beet armyworm |
| CN111328935A (en) * | 2020-04-08 | 2020-06-26 | 四川省农业科学院植物保护研究所 | Feed for beet armyworm and preparation method and application thereof |
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2011
- 2011-02-22 CN CN2011100447859A patent/CN102113471B/en not_active Expired - Fee Related
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN104186430B (en) * | 2014-08-14 | 2016-06-29 | 中国热带农业科学院椰子研究所 | The method of the laboratory test population mass rearing of moth knitted by Cortex cocois radicis |
| CN104206362A (en) * | 2014-09-19 | 2014-12-17 | 贵州大学 | Phytotron for feeding prodenia litura and indoor artificial breeding method |
| CN104757333A (en) * | 2015-04-23 | 2015-07-08 | 中国农业科学院植物保护研究所 | Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method |
| CN105028332A (en) * | 2015-06-10 | 2015-11-11 | 云南农业大学 | Method for shortening culture cycle of Argyrogramma agnata |
| CN106259221A (en) * | 2016-08-19 | 2017-01-04 | 华巧波 | A kind of method raising beet armyworm |
| CN106306389A (en) * | 2016-08-19 | 2017-01-11 | 华巧波 | Fodder for improving breeding efficiency of beet armyworm |
| CN106259221B (en) * | 2016-08-19 | 2019-08-27 | 华巧波 | A method of raising beet armyworm |
| CN111328935A (en) * | 2020-04-08 | 2020-06-26 | 四川省农业科学院植物保护研究所 | Feed for beet armyworm and preparation method and application thereof |
| CN111328935B (en) * | 2020-04-08 | 2022-12-23 | 四川省农业科学院植物保护研究所 | Feed for beet armyworm and preparation method and application thereof |
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