CN106259221A - A kind of method raising beet armyworm - Google Patents

A kind of method raising beet armyworm Download PDF

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Publication number
CN106259221A
CN106259221A CN201610691767.2A CN201610691767A CN106259221A CN 106259221 A CN106259221 A CN 106259221A CN 201610691767 A CN201610691767 A CN 201610691767A CN 106259221 A CN106259221 A CN 106259221A
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powder
beet armyworm
feedstuff
conducive
radix angelicae
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CN106259221B (en
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华巧波
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention provides a kind of method raising beet armyworm, for the different phase of beet armyworm growth, set the different beet armyworm unit are numbers of animals raised, be conducive to its healthy, safety growth;Feedstuff includes following main component: Semen Maydis powder, yeast powder, citric acid, sodium benzoate, sucrose, vitamin C, Folium Mori, Radix Angelicae Sinensis powder, Semen Cassiae powder and water;On the basis of traditional beet armyworm feedstuff, with the addition of Radix Angelicae Sinensis powder and Semen Cassiae powder two Chinese medicine, combine with Folium Mori, beet armyworm immunity can be improved, thus reach to improve its larva survival rate, percentage of pupation and the effect of eclosion rate, after testing, using the beet armyworm of the diet of the present invention, reproductive efficiency can improve more than 10%.

Description

A kind of method raising beet armyworm
Technical field
The present invention relates to insecticide and raise field, especially, relate to a kind of method raising beet armyworm.
Background technology
The lepidoptera pests such as beet armyworm (Spodoptera exigua H ü bner) are class primary pests on vegetable, all Having fulminant, binge, (department rises cloud etc. 2009 to be easily generated the features such as Drug resistance;Liu Xiangyang etc. 2007;Moulton et Al 2000), cause difficulty of prevention and cure to increase, cost accounting improves, and pesticide residues exceed standard, and directly threaten food safety, and it is right to hinder Outer Trade Development, destroys ecological balance (Lu Kai etc. 2005;Huang answers family 2013).
Set up beet armyworm strain, optimize beet armyworm artificial diet technique, it is provided that a large amount of normal developments, neat and consistent For examination worm source, it is insecticide Physiology and biochemistry, the element task of the multinomial research such as entomological genetics and insect toxicology.Insect growth week Phase is short, and population quantity is big, is by the best model organism material of biological study.Artificial from starting calliphora vomitoria in 1908 After feed formula, the research of insecticide artificial breeding progressively grows up.Insect artificial diet is carried out by lot of domestic and international scholar Research, especially most with lepidopteran insects.
Chinese invention patent CN201110386742.9 discloses the feed formula of a kind of beet armyworm, and this Feed Material is easy , low cost, the formula of artificial feed for beet armyworm of beet armyworm can be bred in a large number, but in order to improve beet armyworm further Reproductive efficiency, it is necessary to study a kind of new formula of artificial feed for beet armyworm.
Summary of the invention
The purpose of the present invention is to study a kind of method raising beet armyworm.
A kind of method raising beet armyworm, follows the steps below:
A, product has the ovum paper shears of Beet Armyworm Eggs be cut into the little scraps of paper be placed in sterile petri dish, after ovum blackening, put Enter a small amount of feedstuff, after larva hatches completely, abandon ovum paper;
B, 1-3 raise age in culture dish always, and in each culture dish, larva 250-300 head, puts into feedstuff;
C, after larva length to 3 ages, larva 8-10 head in each culture dish, with brush pen, larva is allocated to other culture dish, Putting into feedstuff, treat long to 4 age ends removing Excreta, interpolation feedstuff, when supporting prepupal period, just feedstuff is eaten up, and is allowed in cultivation Ware is pupated.
Preferably, the described condition raising beet armyworm is: temperature 26 ± 1 DEG C, photoperiod illumination 14h/ dark 10h, Relative humidity is 65-70%R.H..
Preferably, described feedstuff is the feedstuff beneficially improving beet armyworm reproductive efficiency.
Preferably, the described feedstuff being conducive to raising beet armyworm reproductive efficiency, including the one-tenth of following percentage by weight Point:
Semen Maydis powder 15-25%
Yeast powder 5-8%
Citric acid 0.1-0.3%
Sodium benzoate 2-5%
Sucrose 3-5%
Vitamin C 0.5-1%
Folium Mori 3-5%
Radix Angelicae Sinensis powder 1-2%
Semen Cassiae powder 0.3-0.6%
Water surplus.
Preferably, the described feedstuff being conducive to raising beet armyworm reproductive efficiency, including the one-tenth of following percentage by weight Point:
Semen Maydis powder 15-20%
Yeast powder 6-8%
Citric acid 0.2-0.3%
Sodium benzoate 3-4%
Sucrose 4-5%
Vitamin C 0.5-0.8%
Folium Mori 3-5%
Radix Angelicae Sinensis powder 1-1.5%
Semen Cassiae powder 0.3-0.5%
Water surplus.
Preferably, the described feedstuff being conducive to raising beet armyworm reproductive efficiency, including the one-tenth of following percentage by weight Point:
Semen Maydis powder 18%
Yeast powder 7%
Citric acid 0.25%
Sodium benzoate 3.5%
Sucrose 4.5%
Vitamin C 0.65%
Folium Mori 4%
Radix Angelicae Sinensis powder 1.25%
Semen Cassiae powder 0.45%
Water surplus.
Preferably, the described feedstuff being conducive to raising beet armyworm reproductive efficiency, including the one-tenth of following percentage by weight Point:
Semen Maydis powder 15%
Yeast powder 6%
Citric acid 0.3%
Sodium benzoate 3%
Sucrose 5%
Vitamin C 0.5%
Folium Mori 5%
Radix Angelicae Sinensis powder 1%
Semen Cassiae powder 0.5%
Water surplus.
Preferably, the described feedstuff being conducive to raising beet armyworm reproductive efficiency, including the one-tenth of following percentage by weight Point:
Semen Maydis powder 15%
Yeast powder 8%
Citric acid 0.2%
Sodium benzoate 4%
Sucrose 4%
Vitamin C 0.8%
Folium Mori 3%
Radix Angelicae Sinensis powder 1.5%
Semen Cassiae powder 0.3%
Water surplus.
Preferably, described yeast powder is feed grade yeast powder.
Feed grade yeast powder refers exclusively to carbohydrates such as starch, molasses and the high concentrated organic waste liquids such as monosodium glutamate, ethanol For primary raw material, through liquid aerlbic culture yeast, and from it ferments, separate the product prepared after yeast thalline drying, Belong to one of single-cell protein.
The method have the advantages that the method raising beet armyworm of the present invention, for beet armyworm growth Different phase, sets the different beet armyworm unit are numbers of animals raised, is conducive to its healthy, safety growth;Employing is conducive to carrying The feedstuff of high beet armyworm reproductive efficiency, on the basis of traditional beet armyworm feedstuff, with the addition of Radix Angelicae Sinensis powder and Semen Cassiae powder Two Chinese medicine, combines with Folium Mori, can improve beet armyworm immunity, thus reach to improve its larva survival rate, percentage of pupation Effect with eclosion rate.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages. The present invention is further detailed explanation below.
Detailed description of the invention
Hereinafter embodiments of the invention are described in detail, but the present invention can limit according to claim and cover Multitude of different ways implement.
Embodiment 1
A kind of method raising beet armyworm, follows the steps below:
A, product has the ovum paper shears of Beet Armyworm Eggs be cut into the little scraps of paper be placed in sterile petri dish, after ovum blackening, put Enter a small amount of feedstuff, after larva hatches completely, abandon ovum paper;
B, 1-3 raise age in culture dish always, and in each culture dish, larva 280, put into feedstuff;
C, after larva length to 3 ages, larva 9 in each culture dish, with brush pen, larva is allocated to other culture dish, puts into Feedstuff, treats long to 4 age ends removing Excreta, and interpolation feedstuff, when supporting prepupal period, just feedstuff is eaten up, and is allowed in culture dish Pupate.
Described feedstuff is the feedstuff beneficially improving beet armyworm reproductive efficiency.
Described is conducive to improving the feedstuff of beet armyworm reproductive efficiency, including the composition of following percentage by weight:
Semen Maydis powder 18%
Yeast powder 7%
Citric acid 0.25%
Sodium benzoate 3.5%
Sucrose 4.5%
Vitamin C 0.65%
Folium Mori 4%
Radix Angelicae Sinensis powder 1.25%
Semen Cassiae powder 0.45%
Water surplus.
Described yeast powder is feed grade yeast powder.
Embodiment 2
A kind of method raising beet armyworm, follows the steps below:
A, product has the ovum paper shears of Beet Armyworm Eggs be cut into the little scraps of paper be placed in sterile petri dish, after ovum blackening, put Enter a small amount of feedstuff, after larva hatches completely, abandon ovum paper;
B, 1-3 raise age in culture dish always, and in each culture dish, larva 300, put into feedstuff;
C, after larva length to 3 ages, larva 8 in each culture dish, with brush pen, larva is allocated to other culture dish, puts into Feedstuff, treats long to 4 age ends removing Excreta, and interpolation feedstuff, when supporting prepupal period, just feedstuff is eaten up, and is allowed in culture dish Pupate.
Described feedstuff is the feedstuff beneficially improving beet armyworm reproductive efficiency.
Described is conducive to improving the feedstuff of beet armyworm reproductive efficiency, including the composition of following percentage by weight:
Semen Maydis powder 15%
Yeast powder 6%
Citric acid 0.3%
Sodium benzoate 3%
Sucrose 5%
Vitamin C 0.5%
Folium Mori 5%
Radix Angelicae Sinensis powder 1%
Semen Cassiae powder 0.5%
Water surplus.
Described yeast powder is feed grade yeast powder.
Embodiment 3
A kind of method raising beet armyworm, follows the steps below:
A, product has the ovum paper shears of Beet Armyworm Eggs be cut into the little scraps of paper be placed in sterile petri dish, after ovum blackening, put Enter a small amount of feedstuff, after larva hatches completely, abandon ovum paper;
B, 1-3 raise age in culture dish always, and in each culture dish, larva 250, put into feedstuff;
C, after larva length to 3 ages, larva 10 in each culture dish, with brush pen, larva is allocated to other culture dish, puts Entering feedstuff, treat long to 4 age ends removing Excreta, interpolation feedstuff, when supporting prepupal period, just feedstuff is eaten up, and is allowed at culture dish In pupate.
Described feedstuff is the feedstuff beneficially improving beet armyworm reproductive efficiency.
Described is conducive to improving the feedstuff of beet armyworm reproductive efficiency, including the composition of following percentage by weight:
Semen Maydis powder 15%
Yeast powder 8%
Citric acid 0.2%
Sodium benzoate 4%
Sucrose 4%
Vitamin C 0.8%
Folium Mori 3%
Radix Angelicae Sinensis powder 1.5%
Semen Cassiae powder 0.3%
Water surplus.
Described yeast powder is feed grade yeast powder.
Comparative example 1
Radix Angelicae Sinensis powder in embodiment 1 and Semen Cassiae powder being removed, other condition is constant simultaneously.
Comparative example 2
Being removed by Radix Angelicae Sinensis powder in embodiment 1, other condition is constant.
Comparative example 3
Being removed by Semen Cassiae powder in embodiment 1, other condition is constant.
Under equal conditions, the feedstuff of embodiment 1-3 and comparative example 1-3 is carried out feeding experiment, is counted as follows According to:
Table 1: beet exigua larvae survival rate, percentage of pupation and eclosion rate data in feeding experiment:
By testing data above it is recognised that after the present invention adds Radix Angelicae Sinensis powder and Semen Cassiae powder, beet exigua larvae is become Motility rate, percentage of pupation and eclosion rate have lifting by a relatively large margin, and the reproductive efficiency general increase of beet armyworm reaches more than 10%.
By testing data above it is recognised that be individually added into Radix Angelicae Sinensis powder or Semen Cassiae powder, than be simultaneously introduced Radix Angelicae Sinensis powder and The effect of Semen Cassiae powder is far short of what is expected.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, that is made any repaiies Change, equivalent, improvement etc., should be included within the scope of the present invention.

Claims (9)

1. the method raising beet armyworm, it is characterised in that comprise the following steps:
A, product has the ovum paper shears of Beet Armyworm Eggs be cut into the little scraps of paper be placed in sterile petri dish, after ovum blackening, put Enter a small amount of feedstuff, after larva hatches completely, abandon ovum paper;
B, 1-3 raise age in culture dish always, and in each culture dish, larva 250-300 head, puts into feedstuff;
C, after larva length to 3 ages, larva 8-10 head in each culture dish, with brush pen, larva is allocated to other culture dish, Putting into feedstuff, treat long to 4 age ends removing Excreta, interpolation feedstuff, when supporting prepupal period, just feedstuff is eaten up, and is allowed in cultivation Ware is pupated.
2. the method raising beet armyworm as claimed in claim 1, it is characterised in that the condition raising beet armyworm is: temperature Spending 26 ± 1 DEG C, photoperiod illumination 14h/ dark 10h, relative humidity is 65-70%R.H..
3. the as claimed in claim 1 method raising beet armyworm, it is characterised in that described feedstuff is sweet for being conducive to improving The feedstuff of dish noctuid reproductive efficiency.
4. the as claimed in claim 3 method raising beet armyworm, it is characterised in that described being conducive to improves beet armyworm The feedstuff of reproductive efficiency, including the composition of following percentage by weight:
Semen Maydis powder 15-25%
Yeast powder 5-8%
Citric acid 0.1-0.3%
Sodium benzoate 2-5%
Sucrose 3-5%
Vitamin C 0.5-1%
Folium Mori 3-5%
Radix Angelicae Sinensis powder 1-2%
Semen Cassiae powder 0.3-0.6%
Water surplus.
5. the as claimed in claim 3 method raising beet armyworm, it is characterised in that described being conducive to improves beet armyworm The feedstuff of reproductive efficiency, including the composition of following percentage by weight:
Semen Maydis powder 15-20%
Yeast powder 6-8%
Citric acid 0.2-0.3%
Sodium benzoate 3-4%
Sucrose 4-5%
Vitamin C 0.5-0.8%
Folium Mori 3-5%
Radix Angelicae Sinensis powder 1-1.5%
Semen Cassiae powder 0.3-0.5%
Water surplus.
6. the as claimed in claim 3 method raising beet armyworm, it is characterised in that described being conducive to improves beet armyworm The feedstuff of reproductive efficiency, including the composition of following percentage by weight:
Semen Maydis powder 18%
Yeast powder 7%
Citric acid 0.25%
Sodium benzoate 3.5%
Sucrose 4.5%
Vitamin C 0.65%
Folium Mori 4%
Radix Angelicae Sinensis powder 1.25%
Semen Cassiae powder 0.45%
Water surplus.
7. the as claimed in claim 3 method raising beet armyworm, it is characterised in that described being conducive to improves beet armyworm The feedstuff of reproductive efficiency, including the composition of following percentage by weight:
Semen Maydis powder 15%
Yeast powder 6%
Citric acid 0.3%
Sodium benzoate 3%
Sucrose 5%
Vitamin C 0.5%
Folium Mori 5%
Radix Angelicae Sinensis powder 1%
Semen Cassiae powder 0.5%
Water surplus.
8. the as claimed in claim 3 method raising beet armyworm, it is characterised in that described being conducive to improves beet armyworm The feedstuff of reproductive efficiency, including the composition of following percentage by weight:
Semen Maydis powder 15%
Yeast powder 8%
Citric acid 0.2%
Sodium benzoate 4%
Sucrose 4%
Vitamin C 0.8%
Folium Mori 3%
Radix Angelicae Sinensis powder 1.5%
Semen Cassiae powder 0.3%
Water surplus.
9. the method raising beet armyworm as claimed in claim 3, it is characterised in that described yeast powder is feed grade yeast Powder.
CN201610691767.2A 2016-08-19 2016-08-19 A method of raising beet armyworm Active CN106259221B (en)

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CN106259221B CN106259221B (en) 2019-08-27

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113455586A (en) * 2021-06-02 2021-10-01 江苏科技大学 Method for improving content of alpha-linolenic acid in prodenia litura

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CN102578432A (en) * 2012-03-27 2012-07-18 董志平 Artificial feed preparation and simplified feeding method for Athelis lepigone larva
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CN102578425A (en) * 2012-03-14 2012-07-18 中国农业科学院植物保护研究所 Artificial diet for Lepidopterous young larvae, preparation method for artificial diet and application of artificial diet
CN102578432A (en) * 2012-03-27 2012-07-18 董志平 Artificial feed preparation and simplified feeding method for Athelis lepigone larva
RU2013109031A (en) * 2013-02-28 2014-09-10 Елена Александровна Панявина DEVICE FOR KEEPING Worms FROM SPREAD
CN104757333A (en) * 2015-04-23 2015-07-08 中国农业科学院植物保护研究所 Artificial feed for asparagus caterpillar, preparation of artificial feed and feeding method

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113455586A (en) * 2021-06-02 2021-10-01 江苏科技大学 Method for improving content of alpha-linolenic acid in prodenia litura

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