CN104206362B - Prodenia litura indoor artificial breeding method - Google Patents
Prodenia litura indoor artificial breeding method Download PDFInfo
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- CN104206362B CN104206362B CN201410480893.4A CN201410480893A CN104206362B CN 104206362 B CN104206362 B CN 104206362B CN 201410480893 A CN201410480893 A CN 201410480893A CN 104206362 B CN104206362 B CN 104206362B
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- prodenia litura
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Abstract
The invention discloses a phytotron for feeding prodenia litura and an indoor artificial breeding method. The method comprises the following steps: putting prodenia litura larvae into the phytotron under a specific condition; selecting natural hosts (tobacco leaf, Chinese cabbage and the like) to feed the prodenia litura larvae; changing fresh hosts everyday and removing faeces and rotten leaves; after the prodenia litura larvae pupate, centrally putting the prodenia litura into an insect rearing box; after adult emergence of the prodenia litura, pairing the prodenia litura according to the female-male ratio of 1 to 1, laying eggs and breeding population. According to the feeding method, the prodenia litura are fed in the manmade phytotron, the feeding technique is simple and easy to operate, low in feeding cost, and high in reproductive rate, and standard health test insects can be obtained in an indoor continuous feeding manner.
Description
Technical field
The present invention relates to a kind of prodenia litura raises phjytotron and indoor artificial propagation method, belong to the raising of insect
Technical field.
Background technology
Prodenia lituraSpodoptera lituraFabricius is subordinate to Lepidoptera, Noctuidae, calls lotus Autographa spp, custom
Name night tiger, five pink bollworms, fiber crops fiber crops worm etc., are one of global important agricultural insects.Worm host plant species up to 109 sections
389 kinds（Mutation）, addiction causes harm wild cabbageBrassica oleracea, Chinese cabbageBrassica campestris
L.spp.Pekinensis(Lour.) Olsson, capsicumCapsicum annuumDeng vegetables and wheatTriticum aestivum, paddy riceOryza sativaDeng cereal crops, can also cause harm tobaccoNicotiana tabacumDeng industrial crops.
With larva food leaf, bud, flower and fruit, full field crop can be eaten up when serious, to agricultural production huge loss is caused.
At present still based on chemical control, not only preventive effect is poor for conventional dose, and its larva also generally generates anti-for prodenia litura
Property, adding the worm larva is often hidden in soil seam, weeds clump, is difficult to contact polypide during dispenser, while chemical control is also easily caused
The quality comparison of the industrial crops such as the edible safety of the vegetables such as Chinese cabbage, wild cabbage and tobacco, causes chemical control increasingly to be chosen
War, is causing harm for effective control prodenia litura, population biology, genecology and the resistance to the action of a drug and improvement to prodenia litura etc.
Basic research is very necessary, in consideration of it, a large amount of indoors, long-term continued propagation Spodoptera litura Fabricius are a weights for scientific experiment
Want link.
At present, with regard to prodenia litura artificial diet technique, the development and utilization of man-made feeds, research ratio are devoted to substantially
It is more ripe, but artificial feeding formula easily collecting is neat, store constant, some compositions are expensive, in addition Spodoptera litura larvae
With binge habit, artificial feeding is difficult to meet indoor feeding needs, Lv Baoqian etc.（2013）By prodenia litura in dactylethrae
Or single head raising is carried out on Tissue Culture Plate, but single head raises labor intensive, and waste feed, and survival rate is low, it is achieved that one
The method for breeding for planting colony's amount reproduction health prodenia litura worm sources indoors is very necessary.
The content of the invention
The purpose of the present invention is：For the upper defect of prior art, there is provided a kind of prodenia litura raises phjytotron
And indoor artificial propagation method, for realize prodenia litura healthy worm source it is extensive, persistently raise for a long time, to overcome existing skill
The deficiency of art.
Technical scheme
A kind of prodenia litura indoor artificial propagation method, the method interior raising in the controlled environment chamber, comprises the following steps：
A, phjytotron are built：Artificial climate chamber device supports worm frame, ventilation fan, hen house lighting controller, LED daylight lamp
Pipe, electric heating installation using oil as medium and temperature and humidity controller, phjytotron imposes a condition：Periodicity of illumination L:D=14 h:10 h, temperature are 23-
33 DEG C, RH be 65-85%；
B, larval phase, raise：Spodoptera litura larvae picks up from tobacco Experimental Base and as experimental population, is positioned over artificial gas
The larva insect box in room is waited, daily timing water spray keeps humidity, and humidity range is 65-85%, and is fed with host, daily more
Renew fresh host（Tobacco leaf, Chinese cabbage etc.）, the absorbent cotton moisturizing of host's petiole removes daily excrement and rotten blade；
C, pupa time：Spodoptera litura larvae is pupated after 2 d, and pupa is concentrated on into insect box soil layer surface, and daily timing water spray is protected
Wet, humidity range is 65-85%；
D, adult stage：After pupa is sprouted wings, adult presses 1:1 pairing is raised in plastic cup, and the source that supplements the nutrients；
E, ovum phase：Ovum grain is collected daily to be placed in culture dish, is covered with preservative film, to ovum grain blackening, remove preservative film, change
Newly hatched larvae is collected into host's blade.
In above-mentioned prodenia litura indoor artificial propagation method, the described cm of larva insect box length 30, the cm of width 20,
Highly 30 cm；Insect box bottom need to spread thick layer about 3-5 cm, sieve and sandy soil layer after ultraviolet disinfection Jing 10 mesh, per box
Foster worm 200 ~ 500, is covered tightly with 160 mesh gauzes.
In above-mentioned prodenia litura indoor artificial propagation method, pupa time is positioned over sandy soil top layer in insect box, and spray daily is gone
Ionized water moisturizing, per box 180-230 heads.
In above-mentioned prodenia litura indoor artificial propagation method, the adult is female-male proportion 1:1 with being opposite to 700 mL
In disposal plastic cup, extra-nutrition source is put into the rayon balls that is moistened with 10% hydromel using in plastic cup bottom, uses 160 mesh
Gauze is sealed, and rayon balls is changed daily, prevents from rotting to become to turn sour affecting Adult supplement nutrient, using 160 mesh gauze cover mouths, 160
The convenient spawning of mesh gauze and collection ovum grain.
In above-mentioned prodenia litura indoor artificial propagation method, ovum grain is placed in the training of the cm of diameter 10 for being covered with filter paper moisturizing
Foster ware, is covered with preservative film, and 10 ~ 20 apertures of bundle are ventilated on preservative film, and the ovum of collection is the ovum grain for being produced on the same day, each
Culture dish places 800-1200 grain ovum.
In above-mentioned prodenia litura indoor artificial propagation method, insect box and sandy soil use the front h of palpus Jing ultraviolet disinfections 2
More than, it is easy to pupate.
In above-mentioned prodenia litura indoor artificial propagation method, host needs to be cleaned with clear water before nursing and dries, and petiole is used
Absorbent cotton moisturizing.
A kind of prodenia litura raises phjytotron, including chamber body, and ventilation fan is arranged on the door top of chamber body, supports worm frame equal
It is even to be distributed in chamber body, all to install by insect box in each layer for supporting worm frame, LED fluorescent lamp tube is arranged on to be supported on worm frame and is located at
The surface of correspondence insect box, is designed with temperature and humidity controller in the middle part of foster worm frame, and electric heating installation using oil as medium and humidifier are arranged on supports
On chamber body bottom surface between worm frame, LED fluorescent lamp tube is connected with the hen house lighting controller for being arranged on foster worm frame bottom.
Above-mentioned prodenia litura is raised in phjytotron, and the chamber body is square building type structure.
Above-mentioned prodenia litura is raised in phjytotron, and the foster worm frame is at least 2 rows.
As a result of above-mentioned technical proposal, compared with prior art, the present invention raises artificial by arranging prodenia litura
Climatic chamber, and control its illuminance, temperature and humidity completely can truly simulating natural condition, it is ensured that its natural conditions is suitable to
Prodenia litura grows, and by above-mentioned procreation method, is realizing the quick survival and growth of prodenia litura, the procreation method letter of the present invention
Single, feeding cost is low, and survival rate is high, can persistently raise, and obtains the prodenia litura worm sources of a large amount of stable health.
Description of the drawings
Accompanying drawing 1 is the front view that middle twill noctuid of the present invention raises phjytotron；
Accompanying drawing 2 is the top view that middle twill noctuid of the present invention raises phjytotron.
Specific embodiment
Below in conjunction with the accompanying drawings to the present invention with being described in further detail, but not as any limitation of the invention.
Embodiments of the invention：
1st, the construction of phjytotron
A kind of prodenia litura raises phjytotron, as shown in drawings, including chamber body 1, the chamber body 1 is that square building type is tied
Structure, ventilation fan 2 is arranged on the top of door 10 of chamber body 1, supports worm frame 3 and is evenly distributed in chamber body 1, and the foster worm frame 3 is at least 2 rows,
All install by insect box 4 in each layer for supporting worm frame 3, LED fluorescent lamp tube 6 is arranged on supports on worm frame 3 and be located at correspondence insect box 4
Surface, be designed with temperature and humidity controller 9 at the middle part for supporting worm frame 3, electric heating installation using oil as medium 7 and humidifier 8 be arranged on support worm frame 3 it
Between the bottom surface of chamber body 1 on, LED fluorescent lamp tube 6 is connected with the hen house lighting controller 5 for being arranged on the foster bottom of worm frame 3.
Concrete construction step：
4.2 m × 3.6 m × 3.1 m sides the windowing between one（1.4 m×1.4 m）Room（That is chamber body）Window one
Venetian blind type ventilation fan is installed at angle, is placed by wall in room and is raised frame（2 m×0.4 m×1.8 m）3, three layers of frame is raised, often
Layer places insect box（30 cm×20 cm×30 cm）, raise the LED lamp tube 1 that per layer of top of frame hangs 12W, tube power
By hen house illumination timing controller control（Periodicity of illumination L is set:D=14 h:10 h, daytime 06:00 turns on light, night 20:00 puts out
Lamp）, electric heating installation using oil as medium heating is placed in room and temperature and humidity controller control temperature exists（28±5）DEG C, RH is（75±10）%；
2nd, Spodoptera litura larvae picks up from Guizhou Province Majiang County Lan Ba villages tobacco base, builds in taking back interior in the controlled environment chamber
Vertical experimental population, Spodoptera litura larvae is raised in insect box（30 cm × 20 cm × 30 cm) in, it is positioned over artificial climate
In room, the insect box bottom paving cm of thick layer about 3 sieves and sandy soil layer after ultraviolet disinfection Jing 10 mesh（That is insect box and sandy soil
Layer all needs sterilizing to need more than 2h）, it is easy to pupate, worm 200 ~ 500 is supported per box, covered tightly with 160 mesh gauzes, daily timing water spray is protected
Humidity is held, humidity is（75±10）%, changes daily fresh tobacco leaf, i.e. host, and tobacco variety is conventional variety south river 3（It is real
Plant with testing）, excrement and rotten blade are removed every other day, host needs to be cleaned with clear water before nursing and dries, petiole absorbent cotton
Moisturizing；
3rd, after Spodoptera litura larvae pupates 2 d, pupa is concentrated on into soil layer surface, daily spray deionized water moisturizing, humidity is
（75±10）%, per box 180-230 heads；
4th, after pupa is sprouted wings, adult presses 1:1 pairing is raised in 700 mL disposal plastic cups, and plastic cup bottom is put into and is moistened with
The rayon balls of 10% hydromel supplements the nutrients, and is sealed with 160 mesh gauzes, and cotton balls is changed daily, and rayon balls is changed daily, prevents
Only rotten change is turned sour and affects Adult supplement nutrient, and using 160 mesh gauze cover mouths, 160 mesh gauzes are convenient to lay eggs and collection ovum grain；
5th, ovum grain is collected daily to be placed in the culture dish of the cm of diameter 10 for being covered with filter paper moisturizing, use preservative film（On preservative film
Prick 10 ~ 20 apertures）Capping, to ovum grain blackening, removes preservative film, changes host's blade into and collects newly hatched larvae；
Applicant persistently raises indoors prodenia litura using the inventive method, and it averagely goes through the phase in 33.33 d or so, its
Middle ovum phase, larval phase, prepupal period, pupa time, female adult pest phase, the male imago phase is averagely respectively 3.14 d, 14.10 d, 1.43 d,
9.91 d, 4.74 d, 3.63 d.6 ages of larval phase, each age averagely goes through the phase and is followed successively by 1.00 d, 1.33 d, 1.40 d,
2.50 d, 3.27 d and 4.60 d, average viability is 95.32%；Pupa eclosion rate is 98.77%, average pupa weight 0.3041g.It is female
Hero is than average out to 0.92；Single female egg laying amount average out to 1353.20.Compare existing man-made feeds reproduction technique（Coroner is auspicious etc.,
2014；Lv Baoqian etc., 2013）, cost of the present invention is relatively low, raises simple, raises polypide more healthy.
Claims (6)
1. a kind of prodenia litura indoor artificial propagation method, it is characterised in that the method interior raising in the controlled environment chamber, including under
Row step：
A, phjytotron are built：Periodicity of illumination L:D=14 h:10 h, temperature are 23-33 DEG C, RH is 65-85%；
B, larval phase, raise：Spodoptera litura larvae picks up from tobacco Experimental Base and as experimental population, is positioned over phjytotron
In larva insect box, daily timing water spray keep humidity, humidity range is 65-85%, and is fed with host, described larva
The cm of insect box length 30, the cm of width 20,30 cm of height；Insect box bottom need to spread thick layer for 3-5 cm, the mesh of Jing 10 sieve and
Sandy soil layer after ultraviolet disinfection, worm 200 ~ 500 is supported per box, is covered tightly with 160 mesh gauzes；
C, pupa time：Spodoptera litura larvae is pupated after 2 d, and pupa is concentrated on into insect box soil layer surface, daily the moisturizing of timing water spray,
Humidity range is 65-85%；
D, adult stage：After pupa is sprouted wings, adult presses 1:1 pairing is raised in plastic cup, and the source that supplements the nutrients；
E, ovum phase：Ovum grain is collected daily to be placed in culture dish, is covered with preservative film, to ovum grain blackening, remove preservative film, change into and post
Primary blades collect newly hatched larvae.
2. prodenia litura indoor artificial propagation method according to claim 1, it is characterised in that：Pupa time is positioned over insect box
Interior sandy soil top layer, spray deionized water moisturizing daily, per box 180-230 heads.
3. prodenia litura indoor artificial propagation method according to claim 1, it is characterised in that：The adult is male and female
Ratio 1:1 with being opposite in 700 mL disposal plastic cups, and extra-nutrition source adopts to be put in plastic cup bottom and is moistened with 10% honey
The rayon balls of water, is sealed with 160 mesh gauzes, and rayon balls is changed daily, prevents from rotting to become to turn sour affecting Adult supplement nutrient, is adopted
With 160 mesh gauze cover mouths, the convenient spawning of 160 mesh gauzes and collection ovum grain.
4. prodenia litura indoor artificial propagation method according to claim 1, it is characterised in that：Ovum grain is placed in and is covered with filter
The culture dish of the cm of diameter 10 of paper moisturizing, is covered with preservative film, and 10 ~ 20 apertures of bundle are ventilated on preservative film, the ovum of collection
For the ovum grain for being produced on the same day, each culture dish placement 800-1200 grain ovum.
5. prodenia litura indoor artificial propagation method according to claim 2, it is characterised in that：Insect box and sandy soil make
With front more than the h of palpus Jing ultraviolet disinfections 2.
6. prodenia litura indoor artificial propagation method according to claim 1, it is characterised in that：Host needs to use before nursing
Clear water is cleaned and dried, petiole absorbent cotton moisturizing.
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| CN201410480893.4A CN104206362B (en) | 2014-09-19 | 2014-09-19 | Prodenia litura indoor artificial breeding method |
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| CN201410480893.4A CN104206362B (en) | 2014-09-19 | 2014-09-19 | Prodenia litura indoor artificial breeding method |
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| CN104206362B true CN104206362B (en) | 2017-04-19 |
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| CN106857414A (en) * | 2017-04-06 | 2017-06-20 | 海南省农业科学院植物保护研究所 | The mass rearing method of prodenia litura |
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| CN112825817B (en) * | 2019-11-25 | 2022-03-08 | 北京大学 | Method for indoor feeding and breeding Kallima inachus under full-artificial condition |
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| CN202276679U (en) * | 2011-10-12 | 2012-06-20 | 黄石市恒丰医疗器械有限公司 | Novel artificial climate chamber |
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| JPH09170A (en) * | 1995-04-20 | 1997-01-07 | Noyaku Bio Technol Kaihatsu Gijutsu Kenkyu Kumiai | Artificial feed for nami tentou |
| CN102113471B (en) * | 2011-02-22 | 2012-07-04 | 山东农业大学 | Artificial rearing method of larvae of beet armyworm |
| CN103168753B (en) * | 2013-04-17 | 2014-07-09 | 香格里拉县宜康宝生物科技有限公司 | Method for culturing hepialus larvae indoors |
| CN203353474U (en) * | 2013-06-13 | 2013-12-25 | 天津市葱郁农业科技开发有限公司 | Breeding device suitable for black soldierflies |
| CN204180733U (en) * | 2014-09-19 | 2015-03-04 | 贵州大学 | A kind of insect raises phytotron |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN202276679U (en) * | 2011-10-12 | 2012-06-20 | 黄石市恒丰医疗器械有限公司 | Novel artificial climate chamber |
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