CN112825817B - Method for indoor feeding and breeding Kallima inachus under full-artificial condition - Google Patents

Abstract

The invention provides a method for feeding and breeding Kallima inachus under indoor full-artificial conditions, thereby realizing large-scale breeding of the Kallima inachus and providing a stable source for feeding and breeding the Kallima inachus.

Description

Method for indoor feeding and breeding Kallima inachus under full-artificial condition

Technical Field

The invention belongs to the technical field of indoor insect feeding, and particularly belongs to the field of feeding and breeding methods of Kallima inachus.

Background

Kallima inachus (Kallima inachus) genus Kallima order Kallima general family Kallima order Kallima subfamily Kallima genus Kallima order Kallima, have extremely high ornamental and collection value, it is the special important ornamental butterfly of our country. In recent years, with the development and construction of insect ecological ornamental gardens, butterfly gardens have been developed, the demand of living butterflies is increasing, and wild butterfly populations are far from meeting the demand in terms of quantity and quality. For the Kallima inachus, all the existing feeding and breeding methods in the prior art can only realize open-air or semi-open-air feeding in the original producing area of the Kallima inachus, and are severely limited by factors of seasons (diapause in winter) and regions (limited by producing areas). In a scheme of partial indoor feeding, the fed butterfly cannot even complete a life history, and cannot realize reproduction and break of diapause in winter, which greatly restricts the industrial application of the Kallima inachus.

There is a pressing need in the art for a method that enables scale-up of the breeding of Kallima inachus to provide a stable source of Kallima inachus.

Disclosure of Invention

Through years of research, the inventor establishes a set of method for feeding and propagating passages of Kallima inachus indoors under the completely artificial condition, wherein the key technical problem of how to propagate Kallima inachus indoors effectively is solved, and the invention is completed.

Specifically, the present invention provides, in a first aspect, a method for raising and/or breeding Kallima inachus, all of which are carried out indoors, wherein the method comprises a step of assisting the mating of Kallima inachus by using an air current.

The indoor environment is relative to an open space, an open culture or a semi-open environment of a butterfly natural growth environment, and means that the butterfly is in a totally-enclosed space under a completely artificial condition and does not need to be placed in the natural environment at any stage of a complete life history. The breeding and/or reproduction are carried out indoors, namely the breeding of the complete life history of the butterflies is completed under the condition of full or complete manual work, and the normal reproduction close to the natural state can be realized.

The Kallima inachus undergoes complete metamorphosis development, and therefore the culturing steps which are conventional in the art have different culturing modes in terms of four stages of pupal stage, adult stage, egg stage and larval stage, and these culturing steps and conditions which are known in the art to be suitable for each stage are applicable to the method of the present invention. However, the inventors have unexpectedly found that, during mating in the adult stage of male and female butterflies, if mating is carried out without airflow assistance before and/or at the time of mating, effective mating is difficult to be accomplished by the butterflies, which results in that it is difficult to carry out continuous culture, particularly continuous large-scale culture, of multiple generations of Kallima inachus in an all-indoor or all-artificial condition, and further leads to failure in raising and/or breeding. The method of the invention adopts a means of assisting the mating of the Kallima inachus by airflow, solves the long-standing problem in the field and further completes the invention. The "mating of Kallima inachus by airflow assistance" means that wind generated by artificial airflow acts on a butterfly and/or its activity space before/during the mating period of a male butterfly and a female butterfly, so that air in the environment around the butterfly is in a flowing state. Therefore, the mating environment of the butterfly in the nature can be simulated, so that the butterfly can smoothly and efficiently complete the mating process.

In one embodiment, one or more (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10) male Kallima inachus and one or more (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10) female Kallima inachus are placed in a separate space, preferably, the size of the separate space is (40cm-60cm) (60cm-90cm), such as 40cm 60cm, 50cm 70cm, 60cm 90cm, etc., more preferably, the separate space is a cage, pre-mating or mating.

In another embodiment, wherein the host plant used for feeding said Kallima inachus is a plant of the genus Malva, such as Microtoena pusilla or Microtoena pusilla, preferably, said host plant is a potted plant; also preferably, the Kallima inachus and each host plant are raised in a separate space, preferably, the size of the separate space is (40cm-60cm) (60cm-90cm), such as 40cm 60cm, 50cm 70cm, 60cm 90cm, etc., more preferably, the separate space is a cage.

In another embodiment, the step wherein the airflow assists mating of Kallima inachus comprises generating an airflow with an airflow generating device such that male and/or female Kallima inachus to be mated are in an airflow environment; preferably, the gas stream has a flow velocity of 0.1 m/s to 3m/s, more preferably a flow velocity of 0.5m/s to 2.5m/s, such as a flow velocity of 0.1 m/s, 0.2 m/s, 0.3 m/s, 0.4 m/s, 0.5m/s, 0.6 m/s, 0.7 m/s, 0.8 m/s, 0.9 m/s, 1 m/s, 1.1 m/s, 1.2 m/s, 1.3 m/s, 1.4 m/s, 1.5 m/s, 1.6 m/s, 1.7 m/s, 1.8 m/s, 1.9 m/s, 2 m/s, 2.1 m/s, 2.2 m/s, 2.3 m/s, 2.4 m/s, 2.5m/s, 3m/s, or any value therebetween.

The air flow generated by the air flow generating device may act directly on the Kallima inachus, or may act on the air environment outside the Kallima inachus, with the ultimate purpose of placing the Kallima inachus in an environment with air flowing through the air flow generating device.

The step of mating of the air flow assisted Kallima inachus is performed at a time period before, during and/or after mating of the butterflies, for example, the optimal mating period is preferably 15 to 30 days after emergence, for example, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 days, most preferably 20 days after emergence, and the mating duration is 3 to 24 hours, for example, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 hours.

In addition, the air flow generating device can be used for assisting the mating of the Kallima inachus, and can also be used for improving the air convection of the whole indoor space in other stages in butterfly breeding so as to maintain a uniform temperature and humidity environment.

When the air flow generating device is used, the air flow generating device operates for 10-24 hours per day, for example, any value between any two of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 hours.

The air flow generating device is any device capable of generating air flow, and may have fan blades or no fan blades, and may be a fan or a blower, for example.

In another embodiment, wherein the indoor illumination time is maintained for 8-16 hours/day, such as 8, 9, 10, 11, 12, 13, 14, 15 or 16 hours/day.

In another embodiment, wherein the room temperature is maintained at 20-30 ℃, such as 21 ℃, 22 ℃, 23 ℃, 24 ℃, 25 ℃, 26 ℃, 27 ℃, 28 ℃, 29 ℃, 30 ℃ or any temperature value between any two of the above; and/or

Maintaining a room humidity of 40-80%, preferably 45-75%, 50-70% or 55-65%, such as 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80% or any humidity value between any of the above two points;

in another embodiment, the feed in which Kallima inachus imagoes includes natural feed which is fruits such as grapes, peaches, pears, muskmelons, watermelons, longans, litchis, mangos, orange juice, oranges, and oranges; the artificial feed is honey water added with ethanol, wherein the content of honey in the honey water is 10-30%, such as 10%, 15%, 20%, 25% or 30%, preferably, the artificial feed is a mixture of rice wine and 20% honey water.

In another embodiment, wherein the host plant is grown by the following method:

(1) selecting a cultivation container (such as a flowerpot) without a drain hole or blocking the drain hole of the cultivation container to ensure that the cultivation container cannot permeate water, filling culture soil into the container, for example, filling the culture soil to a half of the height of the container, and digging a planting hole;

(2) taking out the seedlings from the seedling raising pot, and reserving soil around the root systems of the seedlings;

(3) planting the seedling taken out in the step (2) into a cultivation container with culture soil;

(4) filling culture soil into the flowerpot, and compacting; watering until the soil is thoroughly watered, wherein the thorough watering means that the watering is not too fast, and the pot soil can be moistened by water every inch until water seeps from the bottom of the flowerpot;

(5) and (4) rejuvenating the transplanted plants in the step (4) under the condition of avoiding direct strong light, transferring the rejuvenated plants to the sun for culture, keeping the culture temperature at 18-30 ℃, and thoroughly watering the plants after the soil is dried during culture to keep the moisture.

The method can realize the feeding and propagation passage of the Kallima inachus under the completely artificial condition, thereby effectively breaking the limitation of seasons (diapause in winter) and regions (the Kallima inachus can be usually cultivated only in the original place). In the method, the average hatching rate of the eggs is 90%, the average survival rate of the larvae is 80%, the average duration of the larvae is about 35 days, the average pupation rate of the larvae is 80%, the eclosion rate is 80%, the average single-female egg yield is 200, the egg hatching rate is 75%, and the method has the effect similar to the effect of scale feeding of Kallima Kallissima in the open air or the semi-open air. The invention realizes the feeding of the Kallima inachus under the condition of complete manual control, has the characteristics of space saving, convenient and fast management, wide application in non-producing areas and the like, and is suitable for large-scale, long-term and subculture feeding of standard test insects. Because the Kallima inachus is an important ornamental butterfly with characteristics in our country, the method has great significance on the biology, ecology, genetic evolution research, popular science display and commercialization of the Kallima inachus.

Detailed Description

The invention may be further understood by the examples, however, it is to be understood that these examples are not limiting of the invention. Variations of the invention, now known or further developed, are considered to fall within the scope of the invention as described herein and claimed below.

Example full-Artificial indoor feeding of Kallima inachus

Insect sources: the first generation of Kallima inachus comes from a butterfly pandemics domesticating and breeding garden in the Bay of Leshan city, and the subsequent insect source is obtained by self breeding.

A rearing cage: the cage specifications include 40cm x 60cm, 50cm x 70cm and 60cm x 90cm using a memory metal scaffold with 100 mesh face stock.

The illumination providing method comprises the following steps: the lamp tube adopts T5 × 150 warm white; the color temperature is 2939 k; dominant wavelength is 582.1 nm; the rated power is 28 w.

Host plant: potted macrophyll or lobular kale, the source: kaemhan in the kawan area of le shan city, Sichuan province, flowerpot: the No. 6 double-color flowerpot has the caliber of 18cm, the total soil containing capacity of 5 liters and the planting soil containing capacity of 3 liters; the amount of watering was 250 mL.

The host plant culture method is as follows:

in order to prevent the soil from flowing out of the drain hole at the bottom of the flowerpot, small stones, window screens or tiles are needed to cover the drain hole; filling culture soil into a pot to a half position, digging out a planting hole, and digging up soil around the seedling by using a flower shovel to loose, wherein a certain distance is kept between the culture soil and a root system; one hand holds the base part of the seedling plant, and the other hand pushes the bottom of the seedling raising pot upwards to take the seedling out of the seedling raising pot; after the seedling is taken out, the seedling is directly planted into a flowerpot with the cultivated soil without removing the soil around the root system; filling culture soil in the flowerpot to a position 2-3 cm away from the edge of the flowerpot, and compacting; watering thoroughly with a watering can, and paying attention to prevent the seedling from lodging; and (4) placing the transplanted plants in a shade place to avoid direct irradiation of strong light, and moving the plants to the sun for maintenance after the seedlings are revived.

The kalimeris microphylla and the kalimeris macrophylla are warm and humid environments which like half-yin and half-light, direct strong light and accumulated water are forbidden, the growth temperature is suitable for 18-30 ℃, the soil humidity is only required to be kept dry and the soil humidity is only required to be fully poured, and the air humidity is preferably kept to be more than 70%. The lamp tube adopts T5 × 150 warm white; the color temperature is 2939 k; the dominant wavelength was 582.1nm and the illumination was 13 hours. The Mallotus philippinensis host germinates for about 15-20 days and recovers for about 45 days. The germination days of the large-leaf kalimeris indica are the same as the germination days of the small-leaf kalimeris indica, and the recovery period is about 15 days later than that of the small-leaf kalimeris indica.

When the soil is dry, the water is irrigated, when the soil is dry, the soil does not absorb water, the water flows out of the pot bottom quickly, the flowerpot can be placed in a water tray, and the water can permeate the whole flowerpot automatically. Watering is carried out as early as possible in the morning and evening to avoid root rot of the plants. Following the principle of dry and wet, the waist water can only provide a part of water source, so as to delay the watering time.

Feeding space

In the embodiment, a small insect breeding room with a closed space is selected, the length is 3.9 meters, the width is 3.2 meters, and the height is 3.5 meters. In order to realize the dense culture, a self-made bracket is placed in the culture room, and the total height of the bracket is about 180-200 cm; the shelf is divided into an upper layer, a middle layer and a lower layer, wherein the upper layer is about 28-40 cm, the middle layer is about 76-80 cm, and the lower layer is about 76-80 cm; one rack is divided into three layers, a middle layer and a lower layer are used, 2-3 small-size cages (with the size of 40 x 60cm) can be placed on each layer of rack, and butterflies are fed in the cages. Controlling and detecting the temperature and humidity of the culture room, wherein the temperature is kept at 22-30 ℃ and the humidity is kept between 50-70%. The light is irradiated for about 13 h.

Feeding and breeding of Kallima inachus

The Kallima inachus undergoes complete metamorphic development, and thus the culturing step includes four stages of a pupal stage, an adult stage, an egg stage and a larva stage.

(1) Pupal stage

Collecting and checking whether the pupae to be eclosized have damage, cracks or necrosis; preparing a net cage, wherein the net cage adopts a 100-mesh gauze surface material, a net dragon frame is a memory metal support, a fine cotton thread rope with the length of about 10cm and a towel required for hanging pupae are erected in the net cage, pupae handles at the root parts of the pupaes are tied by the rope and transferred into the net cage for hanging, the distance between the pupaes is kept to be not less than 3cm, the temperature is kept to be 23-27 ℃, the humidity is 80%, and the net cage is placed in a ventilation position. The eclosion period is about 10 to 15 days.

After the adult eclosion, the wings are spread for 3-4 hours, and then the adult with the completely spread wings is taken out.

(2) Adult stage

Male and female individuals are identified, adults are then numbered and marked on the wings. Recording butterfly numbers, birth dates and sexes on a recording book, and respectively putting adults in separate cages by sex; when mating, the male and female animals are taken out and put into a special mating cage for mating, and the number, the sex and the emergence date are recorded on the mating cage.

Placing host plant herba Kalimeridis (LAYEMALAN or XIAOYANLAN) and natural or artificial feed in adult rearing cage and mating cage, wherein the natural feed comprises fruits with high water content and sugar content such as fructus Vitis Viniferae, fructus Persicae, fructus Pyri, fructus melo, pulp Citrulli, fructus Lycopersici Esculenti, and fructus Citri Tangerinae; the artificial feed is rice wine and 20% honey water.

In the adult feeding and breeding process, water is regularly supplemented to the adult every day, and the water supplementing mode comprises two modes of spraying and water supplementing by placing a water box. Spraying, namely spraying water on the surface of the cage by using a water spraying kettle three times a day in the morning, at noon and at night; if the water box is adopted for water supplement, the water box only needs to be placed into the cage. The feed was changed every three days.

Whether adults can successfully breed is one of the decisive factors for determining whether butterfly indoor feeding can succeed, and the inventor unexpectedly finds that when the adults are mated, if indoor air flow is insufficient, the adults are difficult to effectively chase and mate, and propagation failure is inevitably caused. After many times of attempts, the inventor creatively discovers that the adults can be promoted to fly and chase after air supply by the fan, so that the adults can be successfully bred. Specifically, two butterflies are placed in two rooms with the same conditions, each cage comprises three adult males and females, and the cages are all 100-mesh gauze cages of 40cm by 60 cm. One room is supplied with air at a wide angle of 180 degrees by a beautiful floor fan, the air supply time is 10 hours, and the other room is kept in a windless state. The experiments were independently repeated 5 times, each time using a new cage of butterflies, and each batch of experiments was run for 15 days during which time it was continuously observed whether the mating was successful. Results as shown in table 1, in the windless room, successful mating was not achieved once, whereas in the windy room, successful mating was achieved and fertilized eggs were produced 4 out of 5 experiments. The results prove that the success rate of mating is greatly improved by the aid of the fan to assist mating.

TABLE 1

In addition, the inventor further researches the wind speed range suitable for butterfly mating, and the specific steps are as follows:

6 cages of butterflies are placed in each room, each cage comprises 3 male and female adults, and the cages are combined by using 100-mesh gauze cages of 40cm by 60 cm. The method comprises the steps of utilizing a American floor fan to supply air at a wide angle of 180 degrees in a mode that the air supply time is 10 hours, detecting the air speed nearby each cage by using a velocimeter so as to accurately measure the airflow environment around each cage butterfly.

Because different cages are different with the floor fan distance far and near, thus formed different wind speed districts, it is different according to the wind speed scope, divide into five wind speed districts with the butterfly feeding district: 0.1-0.5m/s, 0.5-1.0m/s, 1.0-2.5m/s, 2.5m/s-3.0m/s and more than 3.0 m/s. 2-3 cages of butterflies are placed in each group of wind speed areas, the experiment is independently repeated for 3 times, each experiment is replaced by a new butterfly, and after 15 days of each experiment, whether the mating is successful or not is continuously observed in the period.

TABLE 2

Note: the numerical meanings in the tables are: the left side of "/" is the number of cages for successful mating and the right side of "/" is the total number of cages for that wind speed range.

As shown in Table 2, in each repeated experiment, the butterfly in the range of 0.1-3m/s has successfully mated events, wherein the mating success rate is very high in the range of 0.5-2.5m/s, reaching nearly 90%. When the wind speed further exceeded 3.0m/s, the mating event was not successful.

The above results show that there is a suitable range of wind speeds for the mating of Kallima inachus, i.e., 0.1 to 3.0m/s, with an optimum range of 0.5 to 2.5 m/s.

Taking the group which successfully copulates and spawns in the experimental group, taking out the host plant, and putting the host plant into a new host plant. The host plant with fertilized eggs is placed into a separate cage, and the adult number and the egg laying date of mating are marked.

(3) In the egg phase

Maintaining the temperature at 23-27 ℃ and the humidity at 80%, and placing the container in a ventilation place. The incubation period is about 4 to 6 days.

(4) Larval stage

After the larvae are hatched, the larvae are put into host plants for free-ranging or taken out and put into a separate box for separate breeding. Preparing a host plant according to the number of larvae, wherein when the cervus lobus is taken as the host plant, 10-15 pots are needed for each larva from 1-5 years old, and more than 3-4 pieces of cervus lobus are planted in each pot for two years.

The 1 st to 2 nd larvae are small, mainly live at the top of the plant, feed or rest on the back of the leaves, and are easy to fall off. At regular daily inspection (e.g. during 8 o 'clock and half-10 o' clock in the morning), if an individual is dropped, the individual needs to be picked up and put back gently with a tip brush pen, so that the larva can climb up the leaf.

The development period of 2 years is 3-5 days, and when a single branch of the host is gnawed by larvae and two or three leaves are left, the host plant needs to be replaced. When the host plant is replaced, the larva is picked up by a brush pen and slowly transferred to a new blade.

When the larvae reach 3-4 years old, the food intake is gradually increased, and the larvae need to observe twice a day in the morning and afternoon every day to prevent leaves from gnawing and starving. The development period at this stage is 3-5 days.

The development period of the 4 th larva is 4-5 days.

The 5 th instar larvae are exposed to a sudden increase in food intake, taking care to provide enough host plants.

When the larvae move slowly and the food intake is reduced in the end-age period, the dry branches are obliquely inserted into the flowerpot to facilitate the larvae to hang upside down and form pupae.

Through the method, the inventor realizes indoor culture and breeding of the Kallima inachus under the completely artificial condition for the first time in the field, the average hatching rate of eggs is 90%, the average survival rate of larvae is 80%, the average calendar period of the larvae is about 35 days, the average pupation rate of the larvae is 80%, the eclosion rate is 80%, the average single-female egg laying amount is 200 eggs, the egg hatching rate is 75%, and the data are similar to the effect of culturing Kallima inachus in the open air or semi-open air in large scale.

The foregoing embodiments are illustrative of the principles and implementations of the present invention, and are presented solely to aid in the understanding of the principles and concepts underlying the invention. It should be noted that it would be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the principles of the invention, and these modifications and variations also fall within the scope of the invention.

Claims (25)

1. A method for feeding and breeding Kallima inachus indoors under fully artificial conditions, the feeding and breeding both being performed indoors, wherein the method comprises a step of assisting the mating of Kallima inachus by using an air flow; the step of air-flow-assisting the mating of Kallima inachus includes bringing male and female Kallima inachus to be mated in an environment with an air flow using an air flow generating device, and the flow velocity of the air flow is 0.1 m/sec to 3 m/sec.

2. The method of claim 1, wherein the gas stream has a flow velocity of 0.5m/s to 2.5 m/s.

3. The method of claim 1, wherein the gas stream has a flow velocity of 0.1 m/s, 0.2 m/s, 0.3 m/s, 0.4 m/s, 0.5m/s, 0.6 m/s, 0.7 m/s, 0.8 m/s, 0.9 m/s, 1 m/s, 1.1 m/s, 1.2 m/s, 1.3 m/s, 1.4 m/s, 1.5 m/s, 1.6 m/s, 1.7 m/s, 1.8 m/s, 1.9 m/s, 2 m/s, 2.1 m/s, 2.2 m/s, 2.3 m/s, 2.4 m/s, 2.5m/s, 3m/s, or any flow velocity value between the two or more.

4. The method of claim 1, wherein the airflow generating device is a fan or blower.

5. The method of claim 1, wherein the retention time of the gas flow is 10 to 24 hours per day.

6. The method of claim 1 wherein one or more male Kallima inachus and one or more female Kallima inachus are placed in a separate space prior to or at the time of mating.

7. The method of claim 6, wherein the size of the isolated space is (40cm-60cm) x (60cm-90 cm).

8. The method according to claim 1, wherein the host plant used for raising the Kallima inachus is ceruleus merzda or ceruleus merzda.

9. The method of claim 1, wherein the indoor illumination time is maintained at 8-16 hours/day.

10. The method of claim 1, wherein the indoor temperature is maintained at 20-30 ℃.

11. The method of claim 1, wherein the indoor humidity is maintained at 40-80%.

12. The method of claim 1 wherein the feed for adult Kallima inachus includes natural feed and artificial feed.

13. The method of claim 12, wherein the natural feed is grape, peach, pear, melon, watermelon, longan, lychee, mango, orange juice, tangerine, or orange.

14. The method of claim 12, wherein the artificial feed is hydromel supplemented with ethanol.

15. A method according to claim 14, wherein the honey water has a honey content of between 10-30%.

16. The method of claim 15, wherein the artificial feed is a mixture of sake and 20% honey water.

17. The method of claim 8, wherein,

the method for planting the host plant comprises the following steps:

(1) selecting a cultivation container without a drain hole or blocking the drain hole of the cultivation container to prevent the cultivation container from permeating water, filling culture soil into the container, and digging out a planting hole;

(2) taking out the seedlings from the seedling raising pot, and reserving soil around the root systems of the seedlings;

(3) planting the seedling taken out in the step (2) into a cultivation container with culture soil;

(4) filling culture soil into the flowerpot, and compacting; watering until soil is thoroughly watered;

(5) and (4) rejuvenating the transplanted plants in the step (4) under the condition of avoiding direct strong light, transferring the rejuvenated plants to the sun for culture, keeping the culture temperature at 18-30 ℃, and thoroughly watering the plants after the soil is dried during culture to keep the moisture.

18. The method of claim 1, comprising the steps of:

1) placing pupae in a cage for suspension culture, and performing eclosion to obtain imagoes;

2) adopting a kale as a host plant, and feeding adults in a cage;

3) mating the adults by using airflow assistance, and collecting eggs laid after mating; wherein male and female Kallima inachus to be mated are brought into an environment with an air flow having a flow velocity of 0.1 to 3 m/sec by an air flow generating device;

4) incubating under conditions suitable for egg incubation to obtain larvae, and feeding the larvae with a kale as host plant.

19. The method of claim 18, wherein the gas stream has a flow velocity of 0.5m/s to 2.5 m/s.

20. The method of claim 18, wherein the gas stream has a flow velocity of 0.1 m/s, 0.2 m/s, 0.3 m/s, 0.4 m/s, 0.5m/s, 0.6 m/s, 0.7 m/s, 0.8 m/s, 0.9 m/s, 1 m/s, 1.1 m/s, 1.2 m/s, 1.3 m/s, 1.4 m/s, 1.5 m/s, 1.6 m/s, 1.7 m/s, 1.8 m/s, 1.9 m/s, 2 m/s, 2.1 m/s, 2.2 m/s, 2.3 m/s, 2.4 m/s, 2.5m/s, 3m/s, or any flow velocity value between the two or more.

21. The method according to claim 18, wherein the feed in the adult feeding of step 2) comprises a natural feed or an artificial feed.

22. The method of claim 21, wherein the natural feed is grape, peach, pear, melon, watermelon, tomato, or orange.

23. The method of claim 21, wherein the artificial feed is rice wine and 20% honey water.

24. The method of claim 18, wherein in step 2) the adults are replenished daily by spraying water or placing containers containing water in cages.

25. The method according to claim 18, wherein in step 4), the conditions suitable for egg hatching are 23-27 ℃ and 80% humidity.