CN104920308A - Method for artificial breeding of larch sclerodermus sp. and application of larch sclerodermus sp. for prevention of massicus raddei - Google Patents
Method for artificial breeding of larch sclerodermus sp. and application of larch sclerodermus sp. for prevention of massicus raddei Download PDFInfo
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- CN104920308A CN104920308A CN201510358583.XA CN201510358583A CN104920308A CN 104920308 A CN104920308 A CN 104920308A CN 201510358583 A CN201510358583 A CN 201510358583A CN 104920308 A CN104920308 A CN 104920308A
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- larch
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M99/00—Subject matter not provided for in other groups of this subclass
Abstract
The invention discloses a method for artificial breeding of larch sclerodermus sp. and application of larch sclerodermus sp. for prevention of massicus raddei. Drone pupae of Bombus hypocrita are selected as substitute hosts of the larch sclerodermus sp., meanwhile, the breeding conditions are regulated and controlled, and thus the parasitic ability of the bred larch sclerodermus sp. is obviously improved. The larch sclerodermus sp. is applied to prevention of the massicus raddei in the Northeast China region, and the parasitic effect of the indoor larch sclerodermus sp. in the massicus raddei larvae of 1-3 years old is good.
Description
Technical field
The present invention relates to biological control of insect pests technical field, particularly relate to the swell artificial breeding method of leg honeybee (Sclerodermus sp.) and the lucky fourth of larch of the lucky fourth of a kind of larch and to swell the application of leg honeybee in control mountain longicorn.
Background technology
Mountain longicorn (Massicus raddei) belongs to coleoptera (Coleoptera) Cerambycidae (Cerambycidae) mallambyx raddei and belongs to (Massicus).Break out in China Northeast Forest Areas the great borer pest caused disaster in recent years, main harm Quercus liaotungensis (Quercus liaotungensis) and Mongolian oak (Q.mongolica).Mountain longicorn is domestic is distributed in more than 20 provinces comprising Taiwan, is distributed in Japan, Korea, Russia abroad.In China, especially with in Jilin, Liaoning Province and Inner Mongol (Chifeng City) harm the most very.Mountain longicorn bores the harm of moth host xylem with larva, the lighter causes a large amount of timber to lose technique and is worth, cause economic loss very heavy, killed heavier standing forest, host's tree crown branch major part is withered, and trunk is riddled with gaping wounds, tree vigo(u)r is weak, windbreak wood is more, and serious killed wood is withered, can only as firewood carbon material inferior.According to investigations, the insect population that individual plant is maximum reaches nearly 200, nearly 500 of maximum burrow.According to Liaoning Province's measuring and calculating of 1999, the timber that mountain longicorn harm loses value reaches 2,000,000 m
3, direct economic loss reaches 1,200,000,000 yuan.And its harm has the trend increased the weight of year by year.Within 2005, Jilin Province's mountain longicorn distribution area is for being about 300,000 hm
2, occurring area 130,000 hectares, Disaster Area 80,000 hm
2, and have the trend spreading further and increase the weight of to endanger.According to measuring and calculating, reach more than 10 hundred million yuan because mountain longicorn endangers the direct economic loss caused.Mountain longicorn has become the No.1 insect of the natural forest area of China northeast based on oak.Have a strong impact on the performance of the ecological and economic benefit of natural forest based on robur, and then have influence on the enforcement of China's Northeast Forest Areas wildwood protected project.An effective approach of economic security is found to become problem demanding prompt solution to the harm controlling mountain longicorn.
Utilize natural enemy insect biological control trunk borer to be considered to one of the most effective and continuable prophylactico-therapeutic measures, mountain longicorn is no exception.At present, application mallambyx raddei biotype dastarcus helophoroides (Dastarcushelophoroides) and swollen leg honeybee (Sclerodermus) prevent and treat mountain longicorn and achieve noticeable achievement, and wherein swollen leg honeybee class natural enemy mainly comprises swell leg honeybee (Sclerodermus pupariae) and larch Ji fourth of the lucky fourth of Chinese wax and to swell leg honeybee.
The lucky fourth of larch leg honeybee and Chinese wax Ji fourth leg honeybee of swelling of swelling belongs to Hymenoptera (Hymenoptera) Bethylidae (Bethylidae) sclerderm Bethylid (Sclerodermus) together, it is the parasitic wasp (being defined as novel species through molecular biology and Morphological Identification) parasitizing the lucky fourth of larch of discovered in recent years, the female cold resistance of this honeybee is high, search host and attacking ability all very strong, be a kind of very excellent trunk borer natural enemy.
Winter low temperature is that can impact grow successful key factor in release surely from south toward moving northward the natural enemy grown, therefore when moving and growing natural enemy, selects distribution closer to the kind moving into ground, to reduce winter low temperature to the injury of natural enemy as far as possible as far as possible.The lucky fourth of larch leg honeybee of swelling is found in In Kuandian, Liaoning Province, is the most by the kind in north in the swollen leg honeybee found at present, for cold district in winter such as control mountain longicorn, agrilus mali etc. insect advantageously.At present, apply its control mountain longicorn in northeast and the agrilus mali in Xinjiang all achieves good effect, the lucky fourth of larch swells leg honeybee at release district safe overwintering, establishes stable population.
Summary of the invention
The lucky fourth of a kind of larch is the object of the present invention is to provide to swell the artificial breeding method of leg honeybee and the application at control mountain longicorn, to swell leg bee imago storing, to be applied in the control of mountain longicorn by adopting the method for artificial breeding to breed the lucky fourth of larch.
For achieving the above object, the present invention proposes following technical scheme: the lucky fourth of a kind of larch swells the artificial breeding method of leg honeybee, it is characterized in that, comprises the following steps: (one) selects Hosts, (2) honeybee kind is selected, (three) artificial breeding;
Preferably, described selection Hosts comprises the following steps: the drone pupae host as an alternative selecting Bombus hypocrita (Bombushypocrita);
Preferably, described selection honeybee kind comprises the following steps: select healthy and be honeybee kind through the lucky fourth of larch of hypergamasis leg honeybee queen bee of swelling;
Preferably, described artificial breeding comprises the following steps:
(1) drone pupae of Bombus hypocrita (Bombus hypocrita) is put into dactylethrae, then access is healthy and to swell leg honeybee queen bee through the lucky fourth of larch of hypergamasis, with the absorbent cotton jam-pack finger-type mouth of pipe, the swell number ratio of leg honeybee queen bee and described Bombus hypocrita drone pupae of the lucky fourth of described larch is 1: 2-1: 3;
(2) dactylethrae is placed on 21 DEG C-22 DEG C, in the climatic cabinate of relative moisture 50%, illumination condition is the nature low light level, cultivates 7-10 days, then the temperature in climatic cabinate is adjusted to 27 DEG C-28 DEG C, relative moisture 70%, cultivates 24-28 days; The lucky fourth of larch leg honeybee of swelling starts to sprout wings, and is taken out by the swollen leg honeybee after sprouting wings, then select from climatic cabinate, for subsequent use;
Preferably, the lucky fourth of described artificial breeding larch out swells, and to be placed in temperature be 10-12 DEG C to leg bee imago, and humidity is store in the climatic cabinate of 40-50%, and the storage time is no more than 3 months;
Present invention further teaches the lucky fourth of larch to swell the application of leg honeybee in control forest trunk borer, in the low instar larvae phase of forest trunk borer, the leg bee imago that lucky for the larch of storage fourth swollen takes out from low temperature climatic cabinate, be transported to woodland, selective temperature higher than 20 DEG C fine day or cloudy day release, during release, glass tube is placed in be injured plant bottoms or base portion, the mouth of pipe down;
Preferably, described forest trunk borer is mountain longicorn;
Preferably, the lucky fourth of described larch swells the burst size of leg bee imago at woodland for discharging than 1: 1 according to benefit evil.
The invention has the beneficial effects as follows:
1. adopt the drone pupae of Bombus hypocrita (Bombus hypocrita) to swell as the lucky fourth of larch the Hosts of leg honeybee, regulate and control Reproduction Conditions simultaneously, the swell parasitic ability of leg honeybee of the lucky fourth of the larch that breeding is obtained is significantly improved; The lucky fourth of application larch swells the mountain longicorn of leg honeybee control the Northeast, and the lucky fourth of the indoor larch parasitic effects of leg honeybee to 1-3 mountain longicorn in age larva that swell is good.
Embodiment
Clear, complete description is carried out to the technical scheme of the embodiment of the present invention below:
Embodiment 1
1, test method: (test in triplicate)
(1) drone pupae of Bombus hypocrita (Bombus hypocrita) is put into dactylethrae, then access is healthy and to swell leg honeybee queen bee through the lucky fourth of larch of hypergamasis, with the absorbent cotton jam-pack finger-type mouth of pipe, the swell number ratio of leg honeybee queen bee and described Bombus hypocrita drone pupae of the lucky fourth of described larch is 1: 2;
(2) dactylethrae is placed on 21 DEG C, in the climatic cabinate of relative moisture 50%, illumination condition is the nature low light level, cultivates 10 days, then the temperature in climatic cabinate is adjusted to 27 DEG C, relative moisture 70%, cultivates 26 days; The lucky fourth of larch leg honeybee of swelling starts to sprout wings, and is taken out by the swollen leg honeybee after sprouting wings, then select from climatic cabinate, for subsequent use;
(3) the white plastic box of 15 30cm × 30cm × 20cm is selected, the robur juggle that 5 sections are connected to mountain longicorn larva (1-3 age) is all put in every box, put into plastic casing by breeding the lucky fourth of the larch obtained leg honeybee of swelling in step (2), the swell number ratio of leg honeybee and mountain longicorn larva of the lucky fourth of larch is 1: 1;
(4) parallel control is arranged, the drone pupae of Bombus hypocrita is replaced to respectively poplar ten spot flatheaded borer larva, paranthrene tabaniformis larva, then above-mentioned steps (1), (2), (3) are repeated respectively, obtain contrast 1 (poplar ten spot flatheaded borer larva), contrast 2 (paranthrene tabaniformis larvas);
(5) putting into 25 DEG C by connecing the plastic casing after honeybee, in the artificial incubator of relative moisture 75%, cutting the lucky fourth of larch that tree skin layer adds up different host breeding after 7-10 days open and to swell the sneak case of leg honeybee.
The lucky fourth of larch of table 1 different host breeding swells the parasitic rate of leg honeybee to 1-3 mountain longicorn in age larva
The selection of host egg of the present invention makes to breed the lucky fourth of the larch the obtained parasitic effects of leg honeybee to mountain longicorn larva that swell and significantly strengthens.
2, test method: (test in triplicate)
(1) drone pupae of Bombus hypocrita (Bombus hypocrita) is put into dactylethrae, then access is healthy and to swell leg honeybee queen bee through the lucky fourth of larch of hypergamasis, with the absorbent cotton jam-pack finger-type mouth of pipe, the swell number ratio of leg honeybee queen bee and described Bombus hypocrita drone pupae of the lucky fourth of described larch is 1: 2;
(2) dactylethrae is placed on 21 DEG C, in the climatic cabinate of relative moisture 50%, illumination condition is the nature low light level, cultivates 9 days, then the temperature in climatic cabinate is adjusted to 28 DEG C, relative moisture 70%, cultivates 27 days; The lucky fourth of larch leg honeybee of swelling starts to sprout wings, and is taken out by the swollen leg honeybee after sprouting wings, then select from climatic cabinate, for subsequent use;
(3) the white plastic box of 15 30cm × 30cm × 20cm is selected, the robur juggle that 5 sections are connected to mountain longicorn larva (1-3 age) is all put in every box, put into plastic casing by breeding the lucky fourth of the larch obtained leg honeybee of swelling in step (2), the swell number ratio of leg honeybee and mountain longicorn larva of the lucky fourth of larch is 1: 1;
(4) parallel control is arranged, and the Reproduction Conditions in set-up procedure (2), is adjusted to 26 DEG C by temperature, relative moisture 60%, and constant temperature and humidity is bred, and other conditions are constant, obtains contrast 3;
Reproduction Conditions in set-up procedure (2), is adjusted to 25 DEG C by temperature, relative moisture 70%, and constant temperature and humidity is bred, and other conditions are constant, obtains contrast 4;
(5) putting into 25 DEG C by connecing the plastic casing after honeybee, in the artificial incubator of relative moisture 75%, cutting the lucky fourth of larch that tree skin layer adds up different host breeding after 7-10 days open and to swell the sneak case of leg honeybee.
The lucky fourth of the larch of table 2 different reproductive condition swells the parasitic rate of leg honeybee to 1-3 mountain longicorn in age larva
The selection of Reproduction Conditions of the present invention can significantly improve breeds the lucky fourth of the larch obtained and to swell the parasitic effects of leg honeybee to mountain longicorn larva.
Claims (8)
1. the lucky fourth of larch swells an artificial breeding method for leg honeybee, it is characterized in that, comprises the following steps: (one) selects Hosts, (two) selection honeybee kind, (three) artificial breeding.
2. the lucky fourth of larch according to claim 1 swells the artificial breeding method of leg honeybee, and it is characterized in that, described selection Hosts comprises the following steps: the drone pupae host as an alternative selecting Bombus hypocrita (Bombushypocrita).
3. the lucky fourth of larch according to claim 2 swells the artificial breeding method of leg honeybee, and it is characterized in that, described selection honeybee kind comprises the following steps: select healthy and be honeybee kind through the lucky fourth of larch of hypergamasis leg honeybee queen bee of swelling.
4. the lucky fourth of larch according to claim 3 swells the artificial breeding method of leg honeybee, and it is characterized in that, described artificial breeding comprises the following steps:
(1) drone pupae of Bombus hypocrita (Bombus hypocrita) is put into dactylethrae, then access is healthy and to swell leg honeybee queen bee through the lucky fourth of larch of hypergamasis, with the absorbent cotton jam-pack finger-type mouth of pipe, the swell number ratio of leg honeybee queen bee and described Bombus hypocrita drone pupae of the lucky fourth of described larch is 1: 2-1: 3;
(2) dactylethrae is placed on 21 DEG C-22 DEG C, in the climatic cabinate of relative moisture 50%, illumination condition is the nature low light level, cultivates 7-10 days, then the temperature in climatic cabinate is adjusted to 27 DEG C-28 DEG C, relative moisture 70%, cultivates 24-28 days; The lucky fourth of larch leg honeybee of swelling starts to sprout wings, and is taken out by the swollen leg honeybee after sprouting wings, then select from climatic cabinate, for subsequent use.
5. the lucky fourth of larch according to claim 4 swells the artificial breeding method of leg honeybee, it is characterized in that, the lucky fourth of described artificial breeding larch out swells, and to be placed in temperature be 10-12 DEG C to leg bee imago, and humidity is store in the climatic cabinate of 40-50%, and the storage time is no more than 3 months.
6. the lucky fourth of larch described in claim 1-5 any one swells the application of leg honeybee in control forest trunk borer, it is characterized in that, in the low instar larvae phase of forest trunk borer, the leg bee imago that lucky for the larch of storage fourth swollen takes out from low temperature climatic cabinate, be transported to woodland, selective temperature higher than 20 DEG C fine day or cloudy day release, during release, glass tube is placed in be injured plant bottoms or base portion, the mouth of pipe is down.
7. the lucky fourth of larch according to claim 6 swells the application of leg honeybee in control forest trunk borer, and it is characterized in that, described forest trunk borer is mountain longicorn.
8. the lucky fourth of larch according to claim 7 swells leg honeybee preventing and treating the application in forest trunk borer, it is characterized in that, the lucky fourth of described larch swells the burst size of leg bee imago at woodland for discharging than 1: 1 according to benefit evil.
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Cited By (8)
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| CN105918257A (en) * | 2016-04-14 | 2016-09-07 | 伊犁哈萨克自治州林业科学研究院 | Method for artificially breeding Sclerodermus pupariae by using local alternate hosts |
| CN106718395A (en) * | 2016-12-01 | 2017-05-31 | 何振贤 | It is a kind of to discharge the method that the swollen leg honeybee of Kazakhstan prevents and treats Chinese scholar tree control of Apriona swainsoni |
| CN106962298A (en) * | 2017-05-26 | 2017-07-21 | 何振贤 | A kind of method of the grizzled bar longicorn in utilization Kazakhstan Zhong Tuifengfang Zhiduo County |
| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
| CN108450418A (en) * | 2018-02-27 | 2018-08-28 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of method of biological control fall webworms |
| CN109315195A (en) * | 2018-09-25 | 2019-02-12 | 常州市绿化管理指导站 | The biological synthesis control method of three kinds of longicorns and other pests on a kind of plant |
| CN109874750A (en) * | 2019-03-22 | 2019-06-14 | 遵义市林业科学研究所 | A kind of artificial breeding method of tiny golden wasp |
| CN117136913A (en) * | 2023-08-31 | 2023-12-01 | 浙江大学 | Indoor propagation method for grassland caterpillar wasp |
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| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
| CN107114326B (en) * | 2017-05-17 | 2020-07-10 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial breeding method of Monochamus alternatus Hope and application of method in prevention and treatment of trunk-boring pests in forest trees |
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| CN108450418A (en) * | 2018-02-27 | 2018-08-28 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of method of biological control fall webworms |
| CN109315195A (en) * | 2018-09-25 | 2019-02-12 | 常州市绿化管理指导站 | The biological synthesis control method of three kinds of longicorns and other pests on a kind of plant |
| CN109874750A (en) * | 2019-03-22 | 2019-06-14 | 遵义市林业科学研究所 | A kind of artificial breeding method of tiny golden wasp |
| CN109874750B (en) * | 2019-03-22 | 2021-09-03 | 遵义市林业科学研究所 | Artificial breeding method of Cereus |
| CN117136913A (en) * | 2023-08-31 | 2023-12-01 | 浙江大学 | Indoor propagation method for grassland caterpillar wasp |
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