CN107787926A - A kind of artificial breeding method of the swollen leg honeybee of apple little Ji fourths and the application in the small buprestid beetle of apple is prevented and treated - Google Patents
A kind of artificial breeding method of the swollen leg honeybee of apple little Ji fourths and the application in the small buprestid beetle of apple is prevented and treated Download PDFInfo
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- CN107787926A CN107787926A CN201711317761.XA CN201711317761A CN107787926A CN 107787926 A CN107787926 A CN 107787926A CN 201711317761 A CN201711317761 A CN 201711317761A CN 107787926 A CN107787926 A CN 107787926A
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- 241000256844 Apis mellifera Species 0.000 title claims abstract description 76
- 241000254173 Coleoptera Species 0.000 title claims abstract description 33
- 238000009395 breeding Methods 0.000 title claims abstract description 18
- 230000003071 parasitic effect Effects 0.000 claims description 11
- 241000382353 Pupa Species 0.000 claims description 9
- 230000001488 breeding effect Effects 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 4
- 241000238631 Hexapoda Species 0.000 claims description 4
- 238000002224 dissection Methods 0.000 claims description 2
- 230000024241 parasitism Effects 0.000 claims description 2
- 238000011835 investigation Methods 0.000 claims 1
- 230000001418 larval effect Effects 0.000 claims 1
- 241001042463 Paraglenea fortunei Species 0.000 abstract description 2
- 244000070406 Malus silvestris Species 0.000 description 67
- 235000011430 Malus pumila Nutrition 0.000 description 66
- 235000015103 Malus silvestris Nutrition 0.000 description 66
- 241000256837 Apidae Species 0.000 description 6
- 241000317929 Chalcididae Species 0.000 description 5
- 241001415867 Tragopan caboti Species 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 4
- 241001070941 Castanea Species 0.000 description 3
- 235000014036 Castanea Nutrition 0.000 description 3
- 241000027461 Massicus raddei Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 241001124657 Bethylidae Species 0.000 description 2
- 241000501044 Buprestidae Species 0.000 description 2
- 241001481710 Cerambycidae Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000257303 Hymenoptera Species 0.000 description 2
- 206010039710 Scleroderma Diseases 0.000 description 2
- 241000529651 Sclerodermus Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 241001470783 Agrilus Species 0.000 description 1
- 241000977910 Agrilus mali Species 0.000 description 1
- 241001620722 Atanycolus sp. Species 0.000 description 1
- 241000880700 Bombus hypocrita Species 0.000 description 1
- 235000005087 Malus prunifolia Nutrition 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- 241000287509 Piciformes Species 0.000 description 1
- 241001546457 Tetrastichus Species 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012876 topography Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fodder In General (AREA)
Abstract
Present invention is disclosed a kind of artificial breeding method of the swollen leg honeybee of apple little Ji fourths and the application in the small buprestid beetle of apple is prevented and treated, by selecting paralglenes fortunei（Paraglenea fortunei）Larva swollen as apple little Ji fourths the Hosts of leg honeybee, selection suitably breeds condition, successfully realizes the swollen leg honeybee of artificial propagation in scale apple little Ji fourths.
Description
Technical field
The present invention relates to biological control of insect pests technical field, more particularly to a kind of artificial breeding side of the swollen leg honeybee of apple little Ji fourths
Method and the application in the small buprestid beetle of apple is prevented and treated.
Background technology
The small buprestid beetle of apple (Agrilus mali Matsumura) belongs to coleoptera (Coleoptera) Buprestidae
(Buprestidae) narrow lucky fourth category (Agrilus) is widely distributed in China.The nineteen ninety-fives such as Wang Chunxiao report apple little Ji first
Tintinnid invades Xinjiang, and is considered that the apple nursery stock introduced by Shandong in 1993 is brought into.Preventing and treating for the small buprestid beetle of apple, early in
The sixties in last century, Zhang Xuemin, Yuan Fujin etc. successively made the research of Prevention Technique, afterwards about having report during its Prevention Technique
Road, summary are got up, and prophylactico-therapeutic measures has mainly wiped out worm branch bar, smeared decoction, punching injection, chemical spray etc..Xinjiang wild fruit
Woods is distributed in river valley abrupt slope both sides more, and with a varied topography, area is big, and distribution is wide, and the small buprestid beetle harm of apple in addition has disguise, fruit
The prevention and controls commonly used in garden are difficult to carry out and operated in crabapple woods.Since 2000, Yi Li states governments at all levels tissue
Carry out the small buprestid beetle of apple preventing and controlling, prophylactico-therapeutic measures mainly clear up brood tree, wiped out worm branch bar, trunk injection,
Adult stage chemical agent spraying etc. measure, reduce to a certain extent preventing and treating area insect density, but the small buprestid beetle of apple by
The trend that year sprawling and harm aggravate not change.Biological control about the small buprestid beetle of apple, Sun Yi is seen earliest and knows 1979
One kind of year report nibble chalcid fly (Tetrastichus sp.) be up to 36.8% in the parasitic rate on fruit tree that do not spray typically~
53.8%, but this is not attracted much attention.It is though even afterwards it has been reported that woodpecker can be attracted or discharge parasitic wasp to come
The small buprestid beetle of apple is prevented and treated, but is not all put into practice.In the small buprestid beetle of apple after Xinjiang Wild Fruit Forest breaks out 15 years, Liu Aihua etc. (2010,
2011) natural enemy insect of the small buprestid beetle of apple is reported first, it was recently reported that the small buprestid beetle of apple carves handle cocoon honeybee (Atanycolus sp.)
To the Natural control action and its Spatial distribution of the small buprestid beetle of apple.There has been no small using the swollen leg honeybee preventing and treating apple of apple little Ji fourths at present
The report of buprestid beetle.
The swollen leg honeybee (Sclerodermus sp.) of apple little Ji fourths and wide variety of Scleroderma Xiao et Wu and Scleroderma sichuanensis Xiao
Belong to Hymenoptera (Hymenoptera) Bethylidae (Bethylidae) sclerderm Bethylid (Sclerodermus) together, be in recent years
Come the parasitic wasp novel species for parasitizing the small buprestid beetle of apple found, the honeybee female has higher wing rate, search host and attacking ability equal
It is very strong, it is a kind of very excellent trunk borer natural enemy.Our team are directed to the research of forest trunk borer natural enemy always, it
The preceding patent with regard to part achievement in research application, such as CN 201510359085.7, CN 201510358583.X etc..It is small in apple
In the artificial breeding research of the swollen leg honeybees of Ji Ding in the past, it has been found that Hosts Bombus hypocrita bee pupa, Cabot's Tragopan pupa, the chestnut used
Mallambyx raddei larva etc. breed apple little Ji fourths swell leg honeybee when effect it is not ideal enough, this is unfavorable for vast propagation.Based on this, we
Team continually looks for new Hosts paralglenes fortunei (Paraglenea fortunei), and studies it and suitably breed condition,
By numerous studies, and then complete the present invention.
The content of the invention
It is an object of the invention to provide a kind of artificial breeding method of the swollen leg honeybee of apple little Ji fourths, and in preventing and treating apple little Ji fourths
Application in worm, realize the swollen leg honeybee of artificial breeding apple little Ji fourths and stored, control and application.
To achieve the above object, the present invention proposes following technical scheme：
The artificial breeding method of the swollen leg honeybee of a kind of apple little Ji fourths, it is characterised in that comprise the following steps：(1) selection substitutes
Host, (two) artificial breeding, (three) low-temperature storage；
Preferably, described selection Hosts comprise the following steps:The 2-3 instar larvaes of paralglenes fortunei are selected as replacement
Host；
Preferably, described artificial breeding comprises the following steps:
(1) larva of paralglenes fortunei is put into dactylethrae, then access health and the swollen leg of apple little Ji fourths through hypergamasis
Honeybee queen bee, the finger-type mouth of pipe, the quantity ratio of the swollen leg honeybee queen bee of the apple little Ji fourths and the paralglenes fortunei larva are stoppered with absorbent cotton
For 2:1-3:1；
(2) dactylethrae is placed on 24 DEG C -25 DEG C, in relative humidity 65-80% growth cabinet, cultivated 27-29 days, apple
The swollen leg honeybee of little Ji fourths starts to sprout wings, and the swollen leg honeybee of apple little Ji fourths after emergence is taken out from growth cabinet, then selected,
It is standby；
Preferably, described low-temperature storage comprises the following steps:The swollen leg honeybee of apple little Ji fourths after emergence is placed in into temperature is
Stored in 5-8 DEG C of growth cabinet, and storage time is no more than 3 months；
Present invention further teaches swollen application of the leg honeybee in the small buprestid beetle of apple is prevented and treated of apple little Ji fourths, in the children of the small buprestid beetle of apple
Worm phase or pupa time, the swollen leg honeybee of the apple little Ji fourths being stored in 5-8 DEG C of growth cabinet is taken out, be transported to woodland, selection temperature
Fine day of the degree higher than 20 DEG C discharges, and dactylethrae is placed in into aggrieved plant bottoms or base portion during release fixes, and the mouth of pipe is down；
Preferably, apple little Ji fourths swell, and the release of leg honeybee is preceding to investigate the small buprestid beetle insect density of apple, and it is small to randomly select 30 pipe apples
The counting of the swollen leg honeybees of Ji Ding, draws average；
Preferably, the apple little Ji fourths swell burst size of the leg honeybee in woodland according to benefit evil ratio 2:1-3:1 release；
Preferably, in 30 days after apple little Ji fourths swell the release of leg honeybee, by randomly selecting the aggrieved plant in part, dissection is adjusted
Look into by the number of pest of parasitism to investigate parasitic rate.
The beneficial effects of the invention are as follows：
1. using Hosts of the larva of paralglenes fortunei as the swollen leg honeybee of apple little Ji fourths, while condition, apple are bred in regulation and control
The swollen leg honeybee of little Ji fourths is obviously improved to the parasitic rate of host, the honeybee quantity that goes out of single head host.
2. the apple little Ji fourths for breed to obtain swell, the parasitic ability of leg honeybee is significantly improved, and the swollen leg honeybee of apple little Ji fourths prevents
Control the significant effect of the small buprestid beetle of apple.
Embodiment
Clear, complete description is carried out to the technical scheme of the embodiment of the present invention below：
Embodiment 1
1st, test method：(experiment is in triplicate)
(1) 3 instar larvaes of paralglenes fortunei are put into dactylethrae, then access is healthy and the apple little Ji fourths through hypergamasis swell
Leg honeybee queen bee, the finger-type mouth of pipe, the quantity of the swollen leg honeybee queen bee of the apple little Ji fourths and the paralglenes fortunei larva are stoppered with absorbent cotton
Than for 2:1；
(2) dactylethrae is placed on 25 DEG C, in the growth cabinet of relative humidity 75%, cultivated 27 days, apple little Ji fourths swell leg
Honeybee starts to sprout wings, and the swollen leg honeybee of apple little Ji fourths after emergence is taken out from growth cabinet, is then selected, is standby；Period
Daily observation queen bee spawning, hatching, larva, emergence situation, until all sprouting wings, count parasitic rate and the quantity into honeybee；
(3) parallel control is set, and the larva of paralglenes fortunei is substituted for into chalcid fly bumblebee drone pupae, Cabot's Tragopan pupa, chestnut respectively
Mallambyx raddei larva, then repeat the above steps (1), (2) respectively, obtains 1 (chalcid fly bumblebee drone pupae) of control, compares 2 (Cabot's Tragopans
Pupa), compare 3 (mountain longicorn larvas).
The effect that 1 different Hosts of table breed the swollen leg honeybee of apple little Ji fourths compares
The present invention uses the larva of paralglenes fortunei to breed the swollen leg honeybee of apple little Ji fourths, the swollen leg honeybee of apple little Ji fourths as Hosts
Parasitic rate on host, the honeybee quantity that goes out on host are all higher than control.The larva for showing paralglenes fortunei is excellent replacement
Host.
2nd, test method：(experiment is in triplicate)
(1) 3 instar larvaes of paralglenes fortunei are put into dactylethrae, then access is healthy and the apple little Ji fourths through hypergamasis swell
Leg honeybee queen bee, the finger-type mouth of pipe, the quantity of the swollen leg honeybee queen bee of the apple little Ji fourths and the paralglenes fortunei larva are stoppered with absorbent cotton
Than for 2:1；
(2) dactylethrae is placed on 25 DEG C, in the growth cabinet of relative humidity 75%, cultivated 27 days, apple little Ji fourths swell leg
Honeybee starts to sprout wings, and the swollen leg honeybee of apple little Ji fourths after emergence is taken out from growth cabinet, is then selected, is standby；
(3) parallel control is set, and the larva of paralglenes fortunei is substituted for into chalcid fly bumblebee drone pupae, Cabot's Tragopan pupa, chestnut respectively
Mallambyx raddei larva, then repeat the above steps (1), (2) respectively, obtains 4 (chalcid fly bumblebee drone pupaes) of control, compares 5 (Cabot's Tragopans
Pupa), compare 6 (mountain longicorn larvas)；
(4) 15 30cm × 30cm × 20cm white plastic box is selected, being put into 5 sections in every box is connected to the small buprestid beetle of apple
The apple tree juggle of larva (1-3 ages), the swollen leg honeybee of apple little Ji fourths that breeding obtains in step (2), (3) is put into plastic casing, apple
The quantity ratio of the swollen leg honeybee of little Ji fourths and the small buprestid beetle larva of apple is 1:1；
(5) plastic casing after honeybee will be connect and be put into 25 DEG C, in the artificial incubator of relative humidity 75%, tree is splitted after 7-10 days
Cortex counts the sneak case for the swollen leg honeybee of apple little Ji fourths that different hosts breed.
Parasitic rate of the swollen leg honeybee of apple little Ji fourths that 2 different hosts of table breed to the small buprestid beetle larva of apple
The selection that the present invention breeds condition can significantly improve the obtained apple little Ji fourths of breeding and swell leg honeybee to the small buprestid beetle of apple
The parasitic effects of larva.
Claims (8)
- The artificial breeding method of leg honeybee 1. a kind of apple little Ji fourths swell, it is characterised in that comprise the following steps：（One）Selection replacement is posted It is main,（Two）Artificial breeding,（Three）Low-temperature storage.
- The artificial breeding method of leg honeybee 2. apple little Ji fourths according to claim 1 swell, it is characterised in that described selection Hosts comprise the following steps:The 2-3 instar larvaes of paralglenes fortunei are selected as Hosts.
- The artificial breeding method of leg honeybee 3. apple little Ji fourths according to claim 2 swell, it is characterised in that described artificial breeding Educate and comprise the following steps:（1）The larva of paralglenes fortunei is put into dactylethrae, it is female then to access the swollen leg honeybee of healthy and through hypergamasis apple little Ji fourths Honeybee, the finger-type mouth of pipe is stoppered with absorbent cotton, the quantity ratio of the swollen leg honeybee queen bee of the apple little Ji fourths and the paralglenes fortunei larva is 2: 1-3:1；（2）Dactylethrae is placed on 24 DEG C -25 DEG C, in relative humidity 65-80% growth cabinet, cultivated 27-29 days, apple little Ji The swollen leg honeybee of fourth starts to sprout wings, and the swollen leg honeybee of apple little Ji fourths after emergence is taken out from growth cabinet, is then selected, is standby With.
- The artificial breeding method of leg honeybee 4. apple little Ji fourths according to claim 3 swell, it is characterised in that described low temperature storage Deposit and comprise the following steps:The swollen leg honeybee of apple little Ji fourths after emergence is placed in the growth cabinet that temperature is 5-8 DEG C and stored, And storage time is no more than 3 months.
- Application of the leg honeybee in the small buprestid beetle of apple is prevented and treated 5. apple little Ji fourths swell, it is characterised in that in the larval phase of the small buprestid beetle of apple Or pupa time, the swollen leg honeybee of apple little Ji fourths described in claim 4 is taken out from growth cabinet, woodland is transported to, selects temperature Fine day release higher than 20 DEG C, is placed in aggrieved plant bottoms or base portion by dactylethrae during release and fixes, the mouth of pipe is down.
- Application of the leg honeybee in the small buprestid beetle of apple is prevented and treated 6. apple little Ji fourths according to claim 5 swell, it is characterised in that apple Little Ji fourths swell, and the release of leg honeybee is preceding to investigate the small buprestid beetle insect density of apple, and randomly selects the counting of the swollen leg honeybee of 30 pipe apple little Ji fourths, Draw average.
- Application of the leg honeybee in the small buprestid beetle of apple is prevented and treated 7. apple little Ji fourths according to claim 6 swell, it is characterised in that institute State apple little Ji fourths and swell burst size of the leg honeybee in woodland according to benefit evil ratio 2:1-3:1 release.
- Application of the leg honeybee in the small buprestid beetle of apple is prevented and treated 8. apple little Ji fourths according to claim 7 swell, it is characterised in that in In 30 days after the swollen leg honeybee release of apple little Ji fourths, by randomly selecting the aggrieved plant in part, dissection investigation is by the insect number of parasitism Measure to investigate parasitic rate.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711317761.XA CN107787926A (en) | 2017-12-12 | 2017-12-12 | A kind of artificial breeding method of the swollen leg honeybee of apple little Ji fourths and the application in the small buprestid beetle of apple is prevented and treated |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711317761.XA CN107787926A (en) | 2017-12-12 | 2017-12-12 | A kind of artificial breeding method of the swollen leg honeybee of apple little Ji fourths and the application in the small buprestid beetle of apple is prevented and treated |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111084159A (en) * | 2020-01-16 | 2020-05-01 | 逄丽艳 | Method for breeding scleroderma guani by utilizing longhornia cuneata |
| CN112568191A (en) * | 2020-12-09 | 2021-03-30 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificially breeding Chinese wax-narrow Jiding as breeding material of multiple natural enemies |
| CN115024283A (en) * | 2022-06-29 | 2022-09-09 | 贵州师范大学 | Method for preventing and treating dendrobe cyrtotrachelossis larva and pupa by using scleroderma guani and application of method |
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| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
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| JP2007297293A (en) * | 2006-04-28 | 2007-11-15 | Arysta Lifescience Corp | Biological pesticide and insect pest controlling method using the same |
| CN107114326A (en) * | 2017-05-17 | 2017-09-01 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of artificial breeding method of the swollen leg honeybee of Monochamus alternatus Hope and the application in preventing and treating forest trunk borer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111084159A (en) * | 2020-01-16 | 2020-05-01 | 逄丽艳 | Method for breeding scleroderma guani by utilizing longhornia cuneata |
| CN112568191A (en) * | 2020-12-09 | 2021-03-30 | 中国林业科学研究院森林生态环境与保护研究所 | Method for artificially breeding Chinese wax-narrow Jiding as breeding material of multiple natural enemies |
| CN115024283A (en) * | 2022-06-29 | 2022-09-09 | 贵州师范大学 | Method for preventing and treating dendrobe cyrtotrachelossis larva and pupa by using scleroderma guani and application of method |
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