CN114586747B - Method for improving adult period and survival rate of coco nula edodes and rodent - Google Patents
Method for improving adult period and survival rate of coco nula edodes and rodent Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 22
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- 244000060011 Cocos nucifera Species 0.000 claims abstract description 67
- 229960004329 metformin hydrochloride Drugs 0.000 claims abstract description 49
- OETHQSJEHLVLGH-UHFFFAOYSA-N metformin hydrochloride Chemical compound Cl.CN(C)C(=N)N=C(N)N OETHQSJEHLVLGH-UHFFFAOYSA-N 0.000 claims abstract description 49
- XZWYZXLIPXDOLR-UHFFFAOYSA-N metformin hydrochloride Natural products CN(C)C(=N)NC(N)=N XZWYZXLIPXDOLR-UHFFFAOYSA-N 0.000 claims abstract description 49
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention belongs to the field of biological control of agricultural and forestry pests, and relates to a method for simultaneously improving the adult period and survival rate of the coco-heart dinosaur, which uses a melissa aqueous solution containing metformin hydrochloride as supplementary nutrition to feed the adult population of the coco-heart dinosaur, uses coco-heart dinosaur pupa impregnated by the metformin hydrochloride solution as a host, provides impregnated coco-heart dinosaur pupa according to the population number of the coco-heart dinosaur, and can prolong the adult period and improve the survival rate of the coco-heart dinosaur. The invention selects the coconut leaf and the first pupa as parasites, provides the honey water solution containing the metformin hydrochloride with specific concentration for the adult coconut leaf and the first rodent as the supplementary nutrient solution, improves the survival rate of the coconut leaf and the first rodent, prolongs the adult coconut leaf and the first rodent period, improves the application effect of biological prevention and control of palm pests in tropical areas of China, and plays a positive role in protecting the planting safety of palm plant industry.
Description
Technical Field
The invention belongs to the field of biological control of agricultural and forestry pests, relates to a method for improving survival rate of a coco russet at an adult stage and prolonging the adult stage of the coco russet by an artificial method, and in particular relates to a method for feeding the coco russet at the adult stage by using a metformin hydrochloride-containing nutrient solution, so as to improve the survival rate of the coco russet at the adult stage and prolong the adult stage of the coco russet.
Background
Palm plants such as coconut, betel nut and oil palm are important components of the tropical natural ecological system in China, can maintain the stability of natural ecological environment, and provide important supporting effects for improving the yield and income increase of farmers and future flexible employment. Coconut leaf beetles are typical invasive palmaceae plant pests in tropical zone, and are the most important pests in the palmaceae plant industry in China. The adults and the larvae take undeployed pericardial epidermis tissues of coconut and other plants to cause shrinkage and curling of leaves and necrosis of heart leaves, so that the growth of the palm plants such as coconut and betel nut is hindered, the yield is reduced by 60-80%, and most plants in the forest die when serious.
Because the trunk of the palmaceae plant is straight and slender, the fruit tree is up to more than ten meters, the manual application operation is difficult, the cost is high, in addition, the coconut leaf beetle is hidden in the heart leaf to be harmful, the concealment is strong, the chemical pesticide control method for spraying and leaching the plant is adopted, the chemical is difficult to directly contact with the insect body, and the effective control effect is difficult to form. In addition, the long-term use of chemical pesticides can also cause the pests to generate drug resistance, kill natural enemies of the pests, pollute the environment and the like while killing the pests, and also influence the natural ecological environment and the edible safety of planted fruits. Therefore, according to the core theory of biological control, biological control measures of controlling insects by insects have become common knowledge.
The Indian honeybee is a pupa parasitic wasp which is widely used for preventing and controlling the Indian honeybee. After the safety evaluation is carried out, the method is introduced, popularized and applied in tropical regions of China. After the emergence and mating of the bees, the behavior of seeking the host to lay eggs is found, eggs are often laid in the coconut leaf beetles, the eggs develop and hatch in the coconut leaf beetles, the larvae and the pupae take the substances in the coconut leaf beetles as nutrition sources, after the emergence of the adult bees, the wall of the coconut leaf beetles is snapped to fly out, the generation development of the coconut leaf beetles is completed, and meanwhile, the control effect on the coconut leaf beetles is achieved.
The purpose of biologically preventing and controlling the coconut leaf beetles is achieved by propagating the coconut leaf beetles and releasing the coconut leaf beetles to the field, and the method is a feasible method for preventing and controlling the palm pests by using parasitic bees. However, how to effectively improve the survival rate of adult coconut leaf first-class rongalite and prolong the life of adult coconut leaf first-class rongalite is an important technical content of efficient propagation of coconut leaf first-class rongalite.
Disclosure of Invention
The invention aims to provide a method for simultaneously improving the adult period and survival rate of the coconut leaf first-class rongalite, which takes the coconut leaf first-class rongalite as a parasite and provides a nutrient solution containing metformin hydrochloride and honey for the coconut leaf first-class rongalite so as to achieve the effects of improving the survival rate of the coconut leaf first-class rongalite and prolonging the adult period of the coconut leaf first-class rongalite. The method has simple operation flow, is safe and reliable, provides a parasitic wasp source for promoting the quantitative, planning and centralized production of the expansion propagation of the coconut leaf beetle, and provides a large amount of released coconut leaf beetle for preventing and controlling the coconut leaf beetle.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a method for improving the adult period and survival rate of coco nuda rodent, comprising the following steps:
(1) Preparing an adult nutrient solution: preparing 5% -20% honey aqueous solution, weighing metformin hydrochloride powder, dissolving to obtain metformin hydrochloride with concentration of 2×10 -4 ～2.8×10 -4 mg/L of honey aqueous solution to obtain an adult nutrient solution for standby;
(2) Coconut leaf and methyl pupa treatment: according to the concentration of the metformin hydrochloride in the adult nutrient solution, preparing a metformin hydrochloride aqueous solution with the same concentration; taking coconut leaf first pupa, immersing in solution for 2-4 s, taking out, and airing to serve as a parasitic object of the coconut leaf first rodent;
(3) Raising adult coco heart leaf and dinotefuran bees: putting the adult coconut leaf first-class rodent bees to be eclosion into a bee-keeping cage, dipping the adult coconut leaf first-class rodent bees in an adult nutrient solution by using a water-absorbing bearing medium, and then placing the adult coconut leaf first-class rodent bees into the bee-keeping cage, and replacing the adult coconut leaf first-class rodent bees 1 time every 1-3 days; during the period, according to the number of female adults of the coconut leaf and first-class engaged bees in the bee-keeping cage, taking the soaked coconut leaf and first-class pupa, supplying the soaked coconut leaf and first-class pupa according to the number of female adults by 0.5-2 times per day, exposing the coconut leaf and first-class pupa for the parasitic of the coconut leaf and first-class engaged bees, replacing the coconut leaf and first-class pupa according to the number of female adults per day, and placing the replaced parasitic coconut leaf and first-class pupa in the room temperature environment until the parasitic bees emerge.
Further, the adult nutrient solution is stored in a refrigerating environment at the temperature of 1-5 ℃ and has the service life of 30 days.
Further, the coconut leaf pupa is within 2 days of age.
Further, the method comprises the steps of, the number of the replaced coconut palm leaf and the first pupa in the bee cage is three fourths of the number of adult bee colonies of the coconut palm leaf and the first rodent bee colony. Generally, the natural male-female ratio of the brothers ' first-class engaged bees is about 3:1, so that the number of the brothers ' first-class pupae replaced every day is provided by three fourths of the number of adult brothers ' first-class engaged bees. In the replacement process, the non-parasitic coconut leaf first pupa is found and can be delivered to the bee cage of the coconut leaf first rodent bee again.
Further, the water for preparing the aqueous solution is double distilled water or commercially available bottled purified water.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention selects the coconut leaf and the first pupa as hosts, provides the honey aqueous solution containing the metformin hydrochloride with specific concentration for the adult coconut leaf and the first rodent as the supplementary nutrient solution, improves the survival rate of the coconut leaf and the first rodent, prolongs the adult coconut leaf and the first rodent period, has simple operation flow, is safe and reliable, saves labor and labor cost, improves the application effect of biological prevention and control of palm pests in tropical areas of China, and plays a positive role in protecting the planting safety of palm plant industry.
2. The invention adopts double measures of immersing the coconut leaf and the first pupa, improving the nutrition of the adult period and the like, improves the survival rate and the adult period of the adult of the coconut leaf and the first rodent, is beneficial to quantification, standardization and intensification of the expanding propagation of the coconut leaf and the first rodent, and promotes ordered and measurable production.
3. The invention quantitatively controls the supply quantity of the brotherus, adjusts the parasitic preference of the adult brotherus, promotes the gradual release of the parasitic capability, prolongs the adult period of the brotherus, is favorable for accumulating a certain scale and a certain number of physiological indexes under indoor conditions to be consistent into a bee population, expands the brotherus population of the target shelf life, is favorable for quantifying, standardizing and intensively expanding the brotherus, promotes the orderly and measurable production, provides a parasitic bee source for developing a large amount of released brotherus for preventing and controlling brous beetles, and integrally improves the expanding propagation process level of the brous beetles.
Drawings
Figure 1 effect of metformin hydrochloride on survival of adult female coco valvular at different concentrations.
Figure 2 effect of metformin hydrochloride on survival of male Cheng Feng of coco nuda.
Figure 3 effect of metformin hydrochloride on prolonging adult stages of female adult coir after a new wash of coir.
Figure 4 effect of metformin hydrochloride on prolongation of the adult stage of male Cheng Feng of coco nuda.
Detailed Description
The following describes the embodiments of the present invention in further detail with reference to examples. The following examples are illustrative of the invention and are not intended to limit the scope of the invention. The experimental methods, in which specific conditions are not noted in the following examples, are generally conducted under conventional conditions or under conditions recommended by the manufacturer.
The survival rate calculating method comprises the following steps: the ratio of the number of surviving insects on the day to the total number of insects tested was x 100%.
Coconut leaf first-class rodent adult survival standard: the bees cannot be overturned to death in 5s for touching by using a soft brush.
Embodiment one: effect of metformin hydrochloride on survival rate of adult Apis cerana Fabricius
Selecting coconut leaf and pupa within 2 days of age, adding each 7 groups of coconut leaf and pupa into a concentration of 1.25X10 -4 mg/L、2.5×10 -4 mg/L、5×10 -4 mg/L、1×10 -3 mg/L、1.5×10 -3 mg/L、2.5×10 -3 mg/L、3×10 -3 mg/L、4×10 - 3 mg/L and 5X 10 -3 Soaking in metformin hydrochloride solution of mg/L for 3s, taking out, air drying, transferring into plastic finger-shaped tube with diameter of 1cm and length of 10cm, and sealing with cotton ball. Collecting newly emerged adult coconut leaf and first rodent wasp, selecting 10 male and female wasp with complete appendages, putting into the plastic finger-shaped tube, and sealing with cotton balls. Each treatment was performed by dipping the pupae with a medical cotton swab containing 10% honey water (metformin hydrochloride concentration 1.25X10) -4 mg/L、2.5×10 -4 mg/L、5×10 -4 mg/L、1×10 -3 mg/L、1.5×10 -3 mg/L、2.5×10 -3 mg/L、3×10 - 3 mg/L、4×10 -3 mg/L and 5X 10 -3 mg/L) for supplementing nutrition to the adult of the Chouioia cunea Yang. Each treatment was set up with 3 to 8 replicates. The survival condition of male and female worms is observed twice a dayAnd recorded, the results are shown in fig. 1 and fig. 2.
As can be seen from fig. 1, the survival rate of females in the test population was continuously decreased as the age of females was increased under the condition that 10% of honey water (containing metformin hydrochloride at different concentrations) was used as a nutrient solution to feed the coir. Without any supplemental nutrition, all female adults die on day 5. The concentration of metformin hydrochloride in the honey water is 2.5X10 -4 The survival rate of female adults on day 14 of the mg/L treatment group was 76.3% which is far greater than that of other treatment groups (e.g. metformin hydrochloride concentration in honey water of 1.25X10) -4 mg/L treatment) or treatment with 10% honey water alone, the survival rates were 57.7% and 35.4%, respectively; on day 18, metformin hydrochloride concentration in honey water was 2.5X10 -4 The survival rate of female adults is 73.7% in mg/L treatment group, and is also larger than that in other treatment groups (such as melbine hydrochloride concentration of 1.25X10 in honey water - 4 mg/L treatment group, treatment group with 10% honey water alone, survival rates were 19.2% and 1.5%, respectively; on day 23, metformin hydrochloride concentration in honey water was 2.5X10 -4 The survival rate of the mg/L treated group was still 2.6%, while the survival rate of the other treated groups was 0. It is shown that metformin hydrochloride is mixed with 10% honey water as supplementary nutrition, and the concentration of metformin hydrochloride is 2.5X10 -4 When the mg/L is about, the survival rate of female adult bees of the coconut leaf and the first-class rodent bees of each day old can be obviously improved.
As can be seen from fig. 2, the survival rate of the male insects of the test population was continuously decreased as the age of the male adults was increased under the condition that 10% of honey water (containing metformin hydrochloride at different concentrations) was used as a nutrient solution to feed the coir. Without any supplemental nutrition, all male adults die on day 5. The concentration of metformin hydrochloride in the honey water is 2.5X10 -4 The survival rate of male adults on day 13 was 76.5% in the mg/L treatment group, which was far greater than that of other treatment groups (e.g., 1.25X10 metformin hydrochloride concentration in honey water) -4 mg/L treatment) or treatment with 10% honey water alone, the survival rates were 44.4% and 27.9%, respectively; hydrochloric acid in Honey water on day 17Metformin concentration of 2.5X10 -4 The survival rate of male adults in mg/L treatment group was 23.5% and also greater than that in other treatment groups (e.g., metformin hydrochloride concentration in honey water was 1.25X10) - 4 mg/L treatment group, treatment group with 10% honey water alone, survival rates were 9.3% and 3.7%, respectively; on day 23, metformin hydrochloride concentration in honey water was 2.5X10 -4 The survival rate of the mg/L treated group was still 5.9%, while the survival rate of the other treated groups was 0. It is shown that metformin hydrochloride is mixed with 10% honey water as supplementary nutrition, and the concentration of metformin hydrochloride is 2.5X10 -4 When the mg/L is about, the survival rate of male adult bees of the coconut leaf and the first rodent bees of each day old can be obviously improved.
Embodiment two: effect of metformin hydrochloride on prolonging adult period of Cocois latifolia Royal
Selecting coconut leaf and pupa within 2 days of age, adding each 7 groups of coconut leaf and pupa into a concentration of 1.25X10 -4 mg/L、2.5×10 -4 mg/L、5×10 -4 mg/L、1×10 -3 mg/L、1.5×10 -3 mg/L、2.5×10 -3 mg/L、3×10 -3 mg/L、4×10 - 3 mg/L and 5X 10 -3 Soaking in metformin hydrochloride solution of mg/L for 3s, taking out, air drying, transferring into plastic finger-shaped tube with diameter of 1cm and length of 10cm, and sealing with cotton ball. Collecting newly emerged adult coconut leaf and first rodent wasp, selecting 10 male and female wasp with complete appendages, putting into the plastic finger-shaped tube, and sealing with cotton balls. Each treatment was performed by dipping the pupae with 15% honey water containing metformin hydrochloride at the corresponding concentration (metformin hydrochloride is 1.25X10 respectively) using a medical cotton swab -4 mg/L、2.5×10 -4 mg/L、5×10 -4 mg/L、1×10 -3 mg/L、1.5×10 -3 mg/L、2.5×10 -3 mg/L、3×10 -3 mg/L、4×10 -3 mg/L and 5X 10 -3 mg/L) for supplementing nutrition to the adult of the Chouioia cunea Yang. Each treatment group was set with 3 to 10 replicates. The survival time of the male and female worms is observed and recorded twice a day, and the results are shown in fig. 3 and 4.
As can be seen from fig. 3, without supplementation,the adult period of female adult wasps of the coconut leaf first-class wasp is only 1.98 days. After using 15% honey water (containing 2.5X10) -4 mg/L metformin hydrochloride) as nutrient solution for supplementing nutrition, the adult period of female adult of Succinum nata can be prolonged to 18.08 days, and then 15% of honey water (containing 5×10) - 4 mg/L metformin hydrochloride) is used as nutrient solution to feed the supplementary nutrition, the adult period of female adult bees of the coco valonia is 13 days, which is higher than that of a treatment group which only uses 15% of honey water to feed the supplementary nutrition, and the adult period of female adult bees is 11.42 days. It is shown that metformin hydrochloride is mixed with 15% honey water as supplementary nutrition, and the concentration of metformin hydrochloride is 2.5X10 - 4 mg/L or 5×10 -4 When the mg/L is about, the adult period of female adult bee of the coconut leaf first-class rodent bee can be prolonged.
As can be seen from fig. 4, the male Cheng Feng adult stage of the brothers, of the dinoflagellates without nutritional supplementation is only 1.85 days. After using 15% honey water (containing 2.5X10) -4 mg/L metformin hydrochloride) is used as nutrient solution to feed the supplementary nutrition, the male Cheng Feng adult period of the coco green shell of the calyx seu fructus physalis is longest, can be prolonged to 14.24 days, and is higher than that of a treatment group which only uses 15% honey water to feed the supplementary nutrition, and the adult period of the male adult bee is 10.89 days. While others are equal to or higher than 5 x 10 -4 The metformin hydrochloride and 10% honey water treatment group with the concentration of mg/L can shorten the adult period of the male Cheng Feng of the Amomum brothers. The results showed that metformin hydrochloride was mixed with 15% honey water as a supplementary nutrition and the concentration of metformin hydrochloride was 2.5X10 -4 When the mg/L is about, the adult period of male adult wasps of the coconut leaf first-class rodent wasps can be prolonged.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the scope of the invention.
Claims (5)
1. A method for improving the adult period and survival rate of a coco nuda rodent, comprising the following steps:
(1) Preparing an adult nutrient solution: preparing 5% -20% honey aqueous solution, weighing metformin hydrochloride powder, dissolving to obtain metformin hydrochloride with concentration of 2×10 -4 ～2.8×10 -4 mg/L of honey aqueous solution to obtain an adult nutrient solution for standby;
(2) Coconut leaf and methyl pupa treatment: according to the concentration of the metformin hydrochloride in the adult nutrient solution, preparing a metformin hydrochloride aqueous solution with the same concentration; taking coconut leaf and methyl pupa, putting into metformin hydrochloride aqueous solution for soaking treatment for 2-4 s, taking out, and taking out after airing as a parasitic object of the coconut leaf and methyl rodent;
(3) Raising adult coco heart leaf and dinotefuran bees: putting the adult coconut leaf first-class rodent bees to be eclosion into a bee cage, dipping the adult coconut leaf first-class rodent bees in an adult bee cage by a water-absorbing bearing medium, and then placing the adult coconut leaf first-class rodent bees in the bee cage, and replacing the adult coconut leaf first-class rodent bees 1 time every 1-3 days; during the period, according to the female adult number of the coconut leaf and first-class oophaga in the bee-keeping cage, taking the soaked coconut leaf and first-class oophaga, supplying the soaked coconut leaf and first-class oophaga according to the female adult number by 0.5-2 times per day, exposing the coconut leaf and first-class oophaga for parasitism of the coconut leaf and first-class oophaga, replacing the coconut leaf and first-class oophaga according to the female adult number per day, and placing the replaced parasitic coconut leaf and first-class oophaga in the room temperature environment until the parasitic bee eclosion.
2. A method of increasing the adult stage and survival rate of a brothers first-class rodent bee as defined in claim 1, wherein: the adult nutrient solution is stored in a refrigerating environment at the temperature of 1-5 ℃.
3. A method of increasing the adult stage and survival rate of a brothers first-class rodent bee as defined in claim 1, wherein: the coconut leaf pupa is coconut leaf pupa within 2 days of age.
4. A method of increasing the adult stage and survival rate of a brothers first-class rodent bee as defined in claim 1, wherein: the number of the replaced coconut palm leaf pupas in the bee-keeping cage is three fourths of the number of female adult bees of the coconut palm leaf first-class rodent bee population.
5. A method of increasing the adult stage and survival rate of a brothers first-class rodent bee as defined in claim 1, wherein: the water for preparing the aqueous honey solution and the aqueous metformin hydrochloride solution is double distilled water or commercially available bottled purified water.
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| JPH05103563A (en) * | 1991-10-11 | 1993-04-27 | Sumitomo Chem Co Ltd | Method for rearing parasitic bee |
| FR2835701A1 (en) * | 2002-02-14 | 2003-08-15 | Univ Rennes | Rearing the parasitic wasp Trybliographa rapae, for use in biological control of Delia flies, especially cabbage flies, by producing fly larvae in cruciferous vegetables then infesting with wasp larvae |
| CN101584420B (en) * | 2009-06-10 | 2011-11-23 | 中国热带农业科学院环境与植物保护研究所 | Brontispa longissima larva artificial feed and preparation method |
| CN102771450A (en) * | 2012-08-14 | 2012-11-14 | 中国热带农业科学院环境与植物保护研究所 | Brontispa longissima mass propagation method |
| CN103109781B (en) * | 2013-03-14 | 2015-06-17 | 吉训聪 | Artificial breeding method of Taiwan abdominal cocoon bee |
| CN107711722B (en) * | 2017-11-02 | 2018-11-06 | 中国热带农业科学院环境与植物保护研究所 | A method of cultivating the Tetrastichus brontispae population with drug resistance |
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