KR20210016843A - Method for artificial hatching of Locusta migratoria - Google Patents

Abstract

The present invention relates to a method for artificial hatching of Locusta migratoria. The artificial hatching method of Locusta migratoria according to the present invention can significantly increase the hatching rate of Locusta migratoria eggs by hatching the eggs under optimum temperature, photoperiod, and illuminance conditions, and thus can mass-produce and ship Locusta migratoria in a planned manner.

Description

Method for artificial hatching of Locusta migratoria}

The present invention relates to an artificial incubation method of velumchi, and more particularly, to a method of artificial incubation of velumchi through adjustment of a specific temperature, photoperiod, and illuminance in order to increase the hatching rate of velumuchi in a short time.

One of the important roles in the natural ecosystem is that insects are used as food for animals, because many insect species are nutrient-rich. In Korea, it has been used as feed for pets about 10 years ago, and has begun breeding in insect lovers and zoos, and recently, due to various advantages as feed insects, there is a trend of mass-proliferating farms for the purpose of industrialization.

Among them, Locusta migratoria is a grasshopper family, usually about 4 to 6 cm in size, and is also a large insect in the grasshopper family, and is known as an edible insect resource that can be consumed by humans or used for animal feed as a source of protein.

In general, bellows hatch from eggs in April-June, and then become imago with wings appearing in July-October. After mating, the adult bellworm puts the pear at a depth of about 10cm in the ground and lays eggs. The spawning of bellows eggs is very sensitive to the environment, so they hatch only when the topography, temperature, and humidity conditions are met. The bellows don't hatch during periods they don't like and keep their eggs, which can last for several years.

Accordingly, the inventors of the present invention have been planning to develop a method for producing vellumchi year-round, while increasing the hatching rate in the state of vellumchi eggs, while confirming the optimum temperature, photoperiod, and illuminance that can be artificially hatched, thereby completing the present invention. I did.

Republic of Korea Patent Registration 10-1867944

The object of the present invention is (a) hatching temperature 30 to 38°C; (b) 55 to 80% humidity; (c) photoperiod 9L/15D to 12L/12D; (d) hatching the eggs of Pulmuchi under conditions of 3,000 to 6,000 Lux of illuminance; It is to provide an artificial hatching method of the velvet containing a.

In order to achieve the above object, the present invention (a) hatching temperature 30 to 38 ℃; (b) 55 to 80% humidity; (c) photoperiod 9L/15D to 12L/12D; (d) hatching the eggs of Pulmuchi under conditions of 3,000 to 6,000 Lux of illuminance; It provides an artificial hatching method of the velvet containing a.

The artificial incubation method of vellumchi according to the present invention can significantly increase the hatching rate of the vellumchi eggs by hatching them at the optimum temperature, photoperiod, and illuminance conditions, so that the vellumchi eggs can be mass produced and shipped intentionally.

1 is a diagram showing the hatching rate of bellows by temperature (meaning that there is a significant difference between the averages not sharing letters).
2 is a diagram showing the daily hatching rate of vellumchi according to temperature.
3 is a diagram showing the hatching rates of velvet velvet at 33° C. and 38° C. as a result of an additional experiment on the hatching rate of velvet velvet according to temperature (Chi-Square test).
FIG. 4 is a diagram showing the hatching rates of velvet velvet at 33° C. and 38° C. as a result of an additional experiment on the hatching rate of velvet chimney according to temperature (T-test).
5 is a diagram showing the daily hatching rates at 33° C. and 38° C. as a result of an additional experiment on the hatching rate of velvet fish according to temperature (Chi-Square test).
6 is a diagram showing the daily hatching rates at 33° C. and 38° C. as a result of an additional experiment on the hatching rate of velvet chimes according to temperature (T-test).
7 is a diagram showing the hatching rate of bellows by humidity.
8 is a diagram showing the daily hatching rate of velumchi according to humidity.
9 is a diagram showing the hatching rate of Pulmuchi by photoperiod.
10 is a diagram showing the daily hatching rate of Pulmuchi according to the photoperiod.
Fig. 11 is a diagram showing the hatching rate of vellumchi by illuminance (meaning that there is a significant difference between the averages not sharing letters).
12 is a diagram showing the daily hatching rate of vellumchi according to the illuminance.
FIG. 13 is a diagram showing the hatching rates of vellums at 3500Lux and 6000Lux as a result of an additional experiment of hatching rates of vellums according to illuminance.
14 is a diagram showing the daily hatching rates at 3500Lux and 6000Lux as a result of an additional experiment on the hatching rate of Pulmuchi according to the illuminance.
Fig. 15 is a diagram showing the hatching rate of velvet velvet in an optimal artificial hatching condition according to the invention.
FIG. 16 is a diagram showing the daily hatching rate under the optimal Pulmuchi artificial hatching condition according to the present invention (means not sharing letters mean significant differences).

Hereinafter, the present invention will be described in detail.

Terms not otherwise defined in the specification have the meanings commonly used in the art to which the present invention belongs.

The present invention (a) hatching temperature 30 to 38 ℃; (b) 55 to 80% humidity; (c) photoperiod 9L/15D to 12L/12D; (d) hatching the eggs of Pulmuchi under conditions of 3,000 to 6,000 Lux of illuminance; It provides an artificial hatching method of the velvet containing a.

All insects are significantly affected by conditions such as temperature, humidity, photoperiod, and illuminance applied during artificial rearing.

In the method of artificial hatching of vellum chi according to the present invention, the temperature condition of the storage environment of vellum egg masses may be 30 to 38°C, more preferably 30 to 33°C, and most preferably 33°C. When stored below 30° C., the hatching rate is somewhat low, and in particular, there is a problem that the velvet eggs are not hatched at 20° C. and 25° C. In addition, when the temperature exceeds 38°C, the temperature is too high, so that the hatching rate of the velvet eggs is lowered and the hatching period is lengthened.

In the method for artificial hatching of vellumchi according to the present invention, the humidity condition of the storage environment of vellum egg masses may be 55 to 80%, more preferably 60 to 70%, and most preferably 65%. When stored at less than 55%, there is a problem that the hatching rate is somewhat low and the hatching period is long. In addition, when the amount exceeds 80%, there is a disadvantage that the incubation time of eggs is delayed.

In the method of artificial hatching of vellumchi according to the present invention, the photoperiod condition of the storage environment of vellum egg masses may be 9L/15D to 12L/12D, and more preferably 9L/15D. When it exceeds 12L/12D, there is a problem that the light irradiation is excessive and the hatching rate is lowered. In addition, when the photoperiod is less than 9L/15D, light irradiation is insufficient, and it is difficult for Pulmuchi eggs to hatch.

In the artificial hatching method of Pulmuchi according to the present invention, the photoperiod condition of the storage environment for Pulmuchi egg masses may be 3,000 to 6,000Lux, more preferably 3,500 to 6,000Lux, and most preferably 3,500Lux. . In the case of irradiation with less than 3,000Lux, the incubation period is delayed, and in particular, in the case of 600Lux, the incubation rate is 28,6%, which significantly decreases the incubation rate. In addition, when the amount exceeds 6,000Lux, the incubation period is somewhat longer.

In one embodiment of the present invention, one of temperature, humidity, photoperiod and illuminance was adjusted and the other conditions were fixed to derive the optimum range for each condition.

In addition, from the above results, the optimum conditions of the artificial incubation method are temperature-33°C, humidity-65%, photoperiod -9L/15D, and illuminance of 3500lux, and incubating the bellows under the above conditions achieves a high hatching rate of 98.82%. In addition, it was confirmed that the hatching period was short from 5 to 7 days, indicating a high hatching rate for a short time.

Therefore, the artificial incubation method of vellumchi according to the present invention exhibits a high hatching rate in a short period of time, so that velumochi can be produced systematically throughout the year, and it is possible to supply materials smoothly according to the increase in the demand for useful insects, and it can be easily used by farmers. As efficiency is expected to increase, it is considered that the commercial use value will be very high.

Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrative purposes only, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not construed as being limited by these examples.

Example 1. Identification of temperature characteristics suitable for improving the hatching rate of velvet

1-1. Experiment method

1) Experimental Insects: 3G, CGN-6G, CHB-7G of Rural Development Administration, egg masses 3-6 days after spawning

2) Experiment temperature: 20℃, 25℃, 27℃, 30℃, 33℃/3 repetition (additional experiment: 33℃, 38℃)

3) Breeding conditions: photoperiod -9L/15D, humidity -65%, illuminance -1800lux

4) Survey period: Once a day, 15 days from the start

5) Survey method: Survey the total hatching rate (15 days) and daily hatching rate. Each egg mass at each temperature was placed in an incubator with a photoperiod -9L/15D, humidity -65%, and illuminance -1800lux, and the hatching rate of eggs separated from hatched larvae and non-hatched egg masses was investigated.

6) Statistical analysis: Oneway ANOVA test, F(2,5.563)=17.647, p=0.004 (total hatching rate); Chi-Square test, T-test (daily hatching rate, additional experiment)

1-2. Experiment result

Experimental results according to Example 1-1 are shown in FIGS. 1 to 6 and Tables 1 to 2 below.

Number of hatchings per day of vellumchi depending on temperature Temperature
(℃) Number of hatching days (days) Total hatching
(Marie) 8 days 9th 10 days 11th 12 days 13th 14 days 15th 20℃ 0 0 0 0 0 0 0 0 0/150 25℃ 0 0 0 0 0 0 0 0 0/150 27℃ 0 8 0 10 7 22 5 12 64/155 30℃ 0 9 0 21 39 18 0 One 88/149 33℃ 14 10 26 70 6 0 0 0 126/149

Daily hatching rate of bellows according to temperature Temperature
(℃) Number of hatching days (days) Total hatch rate
(%) 8 days 9th 10 days 11th 12 days 13th 14 days 15th 20℃ 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 25℃ 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 27℃ 0.0 5.2 0.0 6.5 4.5 14.2 3.2 7.7 41.3 30℃ 0.0 6.0 0.0 14.1 26.2 12.1 0.0 0.7 59.1 33℃ 9.4 6.7 17.4 47.0 4.0 0.0 0.0 0.0 84.6

As shown in Fig. 1, as a result of investigating the hatching rate of Pulmuchi at five temperatures of 20°C, 25°C, 27°C, 30°C and 33°C, 33°C was the highest at 85.6±10.0%, followed by 30°C (63.2) ±24.9%) and 27°C (42.5±9.56%), and since it did not hatch at 20°C and 25°C, it was confirmed that the hatching rate of Pulmuchi was significantly affected by temperature (p=0.004).

In addition, as a result of examining the daily hatching rate according to temperature, it was confirmed that as shown in Fig. 2 and Tables 1 to 2, the lower the temperature, the longer the number of hatching days. In other words, 27℃ hatched between 9-15 days (7 days), 30℃ between 9-13 days (5 days), 33℃ between 8-12 days (5 days) and 33℃ between 10-11 days It was confirmed that the hatching rate was 64.4%.

On the other hand, it was attempted to confirm the optimum temperature condition once again through an additional experiment in a temperature condition higher than 33°C. As a result of investigating the hatching rates at 33°C and 38°C, which have the highest hatching rates, 33°C is 75% (74.8±17.1%), which is 22% higher than 38°C 53% (55.2±23.0%) as shown in FIGS. It was confirmed high. In addition, as shown in FIGS. 5 to 6, in the case of the daily hatching rate, it was confirmed that hatching was performed between 6-8 days (3 days), but at 33°C, the hatching tended to be more concentrated.

From the above results, it is appropriate to store the velvet egg masses at 30 to 38°C, more preferably at 30 to 33°C, and most preferably 33 It was confirmed that it is suitable to store at ℃.

Example 2. Identification of humidity characteristics suitable for improving the hatching rate of Pulmuchi

2-1. Experiment method

1) Experimental Insects: Egg masses at 3-6 days of spawning of CH-9G, CGN-4G, and CHB-7G

2) Experimental humidity: 50% (185), 65% (235), 80% (174) / 4 repetitions

3) Breeding conditions: photoperiod -9L/15D, temperature -30℃, illuminance -1800lux

4) Survey period: Once a day, 15 days from the start

5) Survey method: Survey the total hatching rate (15 days) and daily hatching rate. The specific investigation method is the same as in Example 1-1.

6) Statistical analysis: Oneway ANOVA test, F(2,4.449)=1.730, p=0.278 (total hatch rate); Chi-Square test (daily hatching rate)

2-2. Experiment result

The experimental results according to Example 2-1 are shown in FIGS. 7, 8 and Tables 3 to 4 below.

Daily hatching number of vellumchi depending on humidity Humidity
(%) Number of hatching days (days) Total hatching
(Marie) 7 days 8 days 9th 10 days 11th 12 days 13th 14 days 50% 5 13 24 54 32 3 0 2 133/185 65% 2 17 11 59 69 13 32 0 203/235 80% 0 14 11 46 53 17 0 One 142/174

Daily hatching rate of bellows according to humidity Humidity
(%) Number of hatching days (days) Total hatch rate
(%) 7 days 8 days 9th 10 days 11th 12 days 13th 14 days 50% 2.7 7.0 13.0 29.2 17.3 1.6 0.0 1.1 71.9 65% 0.9 7.2 4.7 25.1 29.4 5.5 13.6 0.0 86.4 80% 0.0 8.0 6.3 26.4 30.5 9.8 0.0 0.6 81.6

As shown in FIG. 7, after fixing the temperature at 30° C., the hatching rate of Pulmuchi was investigated for three humidity levels such as 50%, 65%, and 80%. %(81.6±15.4%), 50%(71.9±14.9%) were found in the order (p=0.278).

In addition, as shown in FIG. 8, as a result of examining the daily hatching rate according to humidity, 65% was 7-13 days (7 days), 80% was 8-14 days (7 days) 1 day later, and 50% was 7 It was confirmed that it hatched for 8 days at -14 days.

Through the above results, it was confirmed that the suitable humidity for the hatching rate of the velvet is 55 to 80%, more preferably 60 to 70%, and most preferably 65%.

Example 3. Identification of photoperiod characteristics suitable for improving hatching rate of Pulmuchi

3-1. Experiment method

1) Experimental Insects: Egg masses 3-6 days after velvety spawning of CGN-6G and CHB-7G

2) Experimental light cycle: 9L/15D (255), 12L/12D (225), 15L/9D (234) / 4 repetitions

3) Breeding conditions: Humidity -65%, temperature -30℃, illuminance -1800lux

4) Survey period: Once a day, 15 days from the start

5) Survey method: Survey the total hatching rate (15 days) and daily hatching rate. The specific investigation method is the same as in Example 1-1.

6) Statistical analysis: Oneway ANOVA test, F(2,9)=0.144, p=0.868 (total hatching rate); Chi-Square test (daily hatching rate)

3-2. Experiment result

The experimental results according to Example 3-1 are shown in FIGS. 9 and 10 and Tables 5 to 6 below.

Number of hatchings of Pulmuchi per day Gwangju period Number of hatching days (days) Total hatching
(Marie) 7 days 8 days 9th 10 days 11th 12 days 13th 14 days 9L/15D 0 30 80 29 59 9 0 0 207/255 12L/12D 0 36 83 37 18 5 One 0 180/225 15L/9D 0 65 45 28 24 8 0 0 170/234

Daily hatching rate of Pulmuchi according to the photoperiod Gwangju period Number of hatching days (days) Total hatch rate
(%) 7 days 8 days 9th 10 days 11th 12 days 13th 14 days 9L/15D 0.0 11.8 31.4 11.4 23.1 3.5 0.0 0.0 81.2 12L/12D 0.0 16.0 36.9 16.4 8.0 2.2 0.4 0.0 80.0 15L/9D 0.0 27.8 19.2 12.0 10.3 3.4 0.0 0.0 72.6

As shown in Fig. 9, after the temperature was fixed at 30°C, the hatching rates of Pulmuchi were investigated for 3 photoperiods such as 9L/15D, 12L/12D, and 15L/9D. , 80.0%, there was little difference, but 15L/9D showed a low hatching rate of 72.6% (p=0.868).

In addition, as shown in Fig. 10, in the case of the daily hatching rate according to the photoperiod, it was confirmed that hatching was performed for 5 days of 8 to 12 days in most of the three photoperiods.

Through the above results, it was confirmed that the photoperiod of 9L/15D to 12L/12D is suitable for artificial hatching of Pulmuchi, and more suitably, storing the Pulmuchi egg mass under 9L/15D conditions.

Example 4. Identification of roughness characteristics suitable for improvement of hatching rate of velvet

4-1. Experiment method

1) Experimental Insects: Egg mass on day 3-6 of vellum scattering of CGN-6G and CHB-7G (Additional experiment: egg mass on day 6 of vellum spawning of GJ-1G, JS-1G, CH-9G)

2) Experimental illuminance: 600lux (80ea), 1800lux (118ea), 3500lux (92ea) / 3 repetitions (Additional experiment: 3500lux (262ea), 6000lux (281ea) / 6 repetitions)

3) Breeding conditions: Humidity -65%, Temperature -30℃, Photoperiod -9L/15D

4) Survey period: Once a day, 15 days from the start

5) Survey method: Survey the total hatching rate (15 days) and daily hatching rate. The specific investigation method is the same as in Example 1-1.

6) Statistical analysis: Oneway ANOVA test, F(2,6)=7.274, p=0.025 (total hatching rate); Chi-Square test, T-test (daily hatching rate, additional experiment)

4-2. Experiment result

The experimental results according to Example 4-1 are shown in Tables 7 and 8, and FIGS. 11 to 14.

Number of hatchings per day of vellum Gwangju period Number of hatching days (days) Total hatching
(Marie) 7 days 8 days 9th 10 days 11th 12 days 13th 600 lux 0 One 14 7 11 4 One 38/80 1800 lux One 27 20 4 20 One 0 73/118 3500 lux 10 39 15 0 6 0 0 70/92

Daily hatching rate of Pulmuchi according to illuminance Gwangju period Number of hatching days (days) Total hatch rate
(%) 7 days 8 days 9th 10 days 11th 12 days 13th 600 lux 0.0 1.3 17.5 8.8 13.8 5.0 1.3 47.5 1800 lux 0.8 22.9 16.9 3.4 16.9 0.8 0.0 61.9 3500 lux 10.9 42.4 16.3 0.0 6.5 0.0 0.0 76.1

As shown in FIG. 11, after fixing the temperature at 30°C, the hatching rate of Pulmuchi was investigated for three illuminances of 600 Lux, 1,800 Lux, and 3,500 Lux. As a result, 3,500 Lux was the highest at 76.1%, followed by 1,800 Lux (61.9 %), 600 Lux (47.5%) was the lowest (p=0.025).

In addition, as a result of examining the daily hatching rate according to the illuminance, as shown in FIG. 12, in the case of 1,800 Lux and 3,500 Lux, it was confirmed that hatching started from the 7th day, and most hatched within 5 days. On the other hand, it was confirmed that 600 Lux started hatching from the 8th day late on the 1st day, and the hatching rate was 28.6% lower than that of 3,500 Lux (Tables 7 to 8).

On the other hand, it was attempted to confirm the optimal illumination condition once again through an additional experiment under illumination conditions higher than 3,500 Lux. As a result of investigating the hatching rate at 3,500 Lux, which has the highest hatching rate and 6,000 Lux, which is the higher illuminance, it was confirmed that as shown in FIG. . In addition, as shown in FIG. 14, in the case of the daily hatching rate, it was confirmed that hatching was performed between 6-8 days (3 days), but 3,500 Lux showed a tendency to hatch more intensively.

Through the above results, in the artificial hatching of Pulmuchi, the illuminance of 3,000 to 6,000 Lux is suitable, more preferably 3,500 to 6,000 Lux is suitable, and most preferably, in case of storing the pelvis egg mass in 3,500 Lux, hatching is performed at once. It was confirmed that a high hatching rate could be achieved while proceeding.

Example 5. Confirmation of the hatching rate of velvet in accordance with the optimal hatching conditions

5-1. Experiment method

The hatching rate was confirmed by hatching Pulmuchi eggs under the optimum temperature, humidity, photoperiod, and illuminance conditions derived in Examples 1 to 4. The specific hatching conditions are as follows.

1) Experimental insects: GJ-1G, egg mass on the 6th day of spawning, 3 repetitions (82, 81, 92 total 255)

2) Breeding conditions: temperature-33℃, humidity-65%, photoperiod-9L/15D, illumination 3500lux

3) Survey period: once a day, 15 days from the start

4) Survey method: Survey the total hatching rate (15 days) and the daily hatching rate. The specific investigation method is the same as in Example 1-1.

5) Statistical Analysis: Oneway ANOVA test, F(2,5.563)=17.647, p=0.004

5-2. Experiment result

The experimental results according to Example 5-1 are shown in FIGS. 15 to 16.

As shown in FIG. 15, as a result of examining the hatching rate and the daily hatching rate at temperature-33°C, humidity-65%, photoperiod-9L/15D, and illuminance 3,500 lux, which were determined as optimal conditions in this experiment, the hatching rate was 98.8% It was confirmed high. In addition, as shown in FIG. 16, it was confirmed that the daily hatching rate was intensively hatched from the 5th day, and all hatched after 3 days.

Through the above results, it was confirmed that temperature -33°C, humidity -65%, photoperiod -9L/15D, and illuminance 3,500 lux can be optimal conditions for artificial incubation of Pulmuchi.

As the specific parts of the present invention have been described in detail above, it is obvious that these specific techniques are only preferred embodiments for those of ordinary skill in the art, and thus the scope of the present invention is not limited thereto. Therefore, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (7)

(a) hatching temperature 30 to 38°C;
(b) 55 to 80% humidity;
(c) photoperiod 9L/15D to 12L/12D;
(d) hatching eggs of Pulmuchi under conditions of 3,000 to 6,000Lux of illuminance; Containing, artificial hatching method of vellum.
The method of claim 1, wherein the hatching temperature is 30 to 33°C.
The method of claim 1, wherein the humidity is 60 to 70%.
The method of claim 1, wherein the photoperiod is 9L/15D.
The method of claim 1, wherein the illuminance is 3,500 to 6,000Lux.
The method of claim 1, wherein the hatching temperature is 33°C, the humidity is 65%, the photoperiod is 9L/15D, and the illuminance is 3,000Lux.
[7] The method of claim 1, wherein the eggs of the velvet have a hatching rate of 80% or more for 5 to 7 days.

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